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Single Nucleotide Polymorphisms In Dna Repair And Oxidative Stress Genes, And Their Interaction With Antioxidants On Prostate Cancer Aggressiveness, Samuel Antwi

Theses and Dissertations

Prostate cancer is the leading invasive malignancy and the second most common cause of cancer death among American men. Despite compelling evidence that oxidative stress, ineffective DNA damage repair, and habitually low antioxidants intake may act in tandem to influence prostate carcinogenesis, few studies have examined gene-diet interactions involving these risk factors. Even fewer studies have examined such interactions in relation to prostate cancer aggressiveness. This study investigated whether single nucleotide polymorphisms (SNPs) in DNA repair- and oxidative stress-related genes modulated associations between antioxidant intake and prostate cancer aggressiveness. We utilized data from the North Carolina-Louisiana Prostate Cancer Project (PCaP) …

Development And Optimization Of A Rapid Assay Kit For The Detection Of Vibrio Cholerae In Bivalves, Demarcus Rashad Carter

Theses and Dissertations

A rapid assay kit for Vibrio cholerae (Vc) was developed to detect and quantify Vc cells in oyster samples within 24 h. The kit, formulated within a two -phase (liquid and solid) 96-well plate, can detect biomarker expression of Vc when the enrichment broth and incubation temperature are optimized. The kit showed 91 % selectivity and 92 % specificity when tested with 23 inclusive Vc and 106 exclusive non-Vc strains. The kit was further optimized using 47 samples of oysters, clams, and soil. There was no significant difference in most probable number between the kit, conventional PCR and BAX PCR …

The Use Of Tonometry As A Diagnostic Tool To Evaluate Intraocular Pressures In Normal And Abnormal California Sea Lion Eyes, Johanna Del Carmen Mejia-Fava

Theses and Dissertations

Ocular disease is one of the most common problems encountered in sea lions at various zoos and aquariums around the world.1 The California sea lion (Zalophus californianus) is one of the most common marine mammals maintained in zoos and is also the most commonly afflicted with ocular disease. Studies have shown that pinnipeds housed in captivity manifest an array of ocular lesions.2 Eye disease can range from a pinpoint corneal opacity to loss of vision due to keratopathy, cataracts and secondary glaucoma. Glaucoma is a disease that has not been extensively studied in the sea lion.3 Observation of clinical signs …

Characterization Of Sediment Microbial Communities And Analysis Of Biogeochemical Responses To Eutrophication In Southeastern Estuaries, Gary Alon Blakeney

Theses and Dissertations

Estuaries are valuable economic resources to humans. However, changes in these ecosystems, such as alteration in nutrient availability, can impose eutrophic pressures. Traditionally, estuaries have been monitored through chemical analyses that measure factors such as sediment oxygen demand (SOD) and reduced forms of Fe(II), Mn(II), and H2S. These methods are time consuming and have been proven to be unreliable. This study was conducted to determine if using T-RFLP analysis could be used as a proxy for some of the current methods used to monitor eutrophication. Using SPSS to perform a principle component analysis, it was determined that connections can be …

Ecosystem Services Of Moist-Soil Wetlands: Water Quality Benefits And Crayfish Harvest, Economics And Consumer Acceptance, Amy Brooke Alford

Theses and Dissertations

I estimated yield, population metrics, production costs, and evaluated consumer acceptability and nutritive content of crayfish (Procambarus clarkii, P. zonangulus) harvested from moist-soil wetlands in the Lower Mississippi Alluvial Valley (MAV) during springs 2009-2011. I also compared nutrient and sediment concentrations and loads exported from moist-soil wetlands and nearest agriculture fields in the MAV during winters 2010-2012. Daily yield of crayfish from moist-soil wetlands was 2.4 kg (wet) ha-1 (SE = 0.50; 95% CI = 1.3-3.4). When graded by size, yield of large crayfish (> 30 g) from wetlands dominated by P. clarkii was four times greater (P < 0.05) than yield of large crayfish from wetlands dominated by P. zonangulus. Crayfish harvesting costs (2013 US dollars [$]) ranged from $405.69 ha-1 to $917.88 ha-1 and breakeven selling prices ranged from $3.74 kg-1 to $8.49 kg-1. Consumer acceptability, proximate composition, and total fatty acid content of P. clarkii did not differ (0.73 > P > 0.11) …

Efficacy Of An Experiential, In-School Educational Program For Improving Elementary School Students' Attitudes And Knowledge About The Environment, Leslie M. Burger

Theses and Dissertations

Stagnant science achievement by students, greater demands and stresses on natural resources and environmental systems, and societal disengagement from nature highlight the need for education programs to ameliorate subsequent consequences. One attempt to address science performance and environmental apathy is Youth Environmental Science (YES), an environmental education program initiated in 2011 in a rural, minority-dominated, upper elementary school in Mississippi. The program provides five consecutive days (30 hours) of experiential learning in natural sciences. During 2011-2013, I studied cognitive and affective responses of fourth and fifth grade students to YES participation using a pretest-posttest-delayed posttest design. The influence of demographic …

Evaluation Of Effectiveness, Reproducibility, And Repeatability Of Using Dentition For Estimating Cattle Age, Timothy F. Best

Theses and Dissertations

Study objectives were to determine the effectiveness, reproducibility, and repeatability of dental evaluation for estimating cattle age. Cattle (n = 400) aged 1.5 to 20 yr were evaluated by 3 technicians for estimated age via dentition. A subset (n = 383) was aged again 4 wk later by the same observers. Age estimations were most accurate in YOUNG (< 5 yr old) cattle with at least 95.7% rate of accuracy within 2 yr across all observers and observation events. For MIDDLE (6 to 10 yr old) and OLD (> 10 yr old) cattle, these accuracies were 81.5 and 62.1%, respectively. Reproducibility proved high, with all observers consistently assigning age estimates within 1 yr of one another for more than 9 out of 10 YOUNG animals; all observers agreed on at …

Use Of Natural Antimicrobials To Control Spoilage In Marinara-Type Sauce, Austin R. Abessinio

Theses and Dissertations

Marinara-type sauces were created using three natural antimicrobials, as well as two combination treatments (natamycin, propionic acid, cultured dextrose, natamycin-propionic acid, and natamycin-cultured dextrose) and two controls (sodium benzoate-potassium sorbate, no preservatives). Samples were subjected to a shelf-life study at 20 C with both non-inoculated sauce and sauces that were either inoculated with Zygosaccharomyces bailii or a cocktail of thermophilic fermentative organisms. Natamycin and Natamycin-propionic acid treatments had fewer log colony counts (CFU/g) of yeast and lactic acid bacteria than the negative control after 42 days of storage and performed as well or better than the positive control throughout the …

Study Of The Mechanisms Of Heat Tolerance In Ivy Geraniums, Mingshu Zhang

Theses and Dissertations

Ivy geranium (Pelargonium peltatum) is a heat susceptible species with its heat tolerance varying among varieties. Reactive oxygen species (ROS) and in-vivo defense systems are related to plant heat damage and heat tolerance. Application of chelated-iron has also been reported to enhance ivy geranium heat tolerance; however, the correlation of ROS, relative enzyme stability, and iron content to differences in heat tolerance in ivy geraniums is unknown. Here we show that the H2O2 content and ROS scavenging enzyme stability in ivy geranium varies with varieties and active iron is not related to heat tolerance in ivy geranium. H2O2 content in …

Investigating Knowledge And Behavior Intention Among Ghanaian Smallholder Farmers, Alyssa Marie Barrett

Theses and Dissertations

Rural farmers in developing countries lack knowledge, access to educational resources, and capacity to stay informed of and implement current farming and health practices. The purpose of this research was to determine the effect of an educational program on the utilization of native plants in farming and health practices among rural farmers in Ghana. The research objectives were to describe the population, assess farmers’ knowledge of farming and health practices, describe participants’ current and planned behavior, and observe the implementation of the practices taught. Results indicated participants’ knowledge of farming and health practices increased after the workshops. Results also indicated …

Investigating Melatonin Supplementation On Maternal Hemodynamics And Offspring Programming, Katelyn E. Brockus

Theses and Dissertations

The objective was to examine effects of melatonin supplementation during late gestation on uterine artery hemodynamics, offspring growth, and endocrine profiles. Prior to day 170 of gestation, heifers were trained to the Calan feeding system. On day 190 of gestation, heifers (n = 20) were blocked by BW and then randomly assigned to one of two dietary treatments: 1) 20 mg of dietary melatonin per day (MEL) or 2) no melatonin supplementation (CON). Supplementation ceased on day 262 of gestation. A main effect (P < 0.01) of treatment was observed for total uterine artery blood flow with it being increased in MEL vs. CON. An interaction (P = 0.008) was observed in calf body weight increasing at weeks 8 and 9 in MEL vs. CON. Dietary melatonin could be used to potentially increase uterine blood flow and calf body weight.

Investigation Of The Beneficial Effect Of Enterobacter Cloacae Strain Jd6301 On Mice Challenged With Escherichia Coli O157:H7, Jessica Grissett Wilson

Theses and Dissertations

The environment of the gastrointestinal (GI) tract is an extremely complex system made up of not only host cells, but also many beneficial microbes. Disruption of this environment can often lead to disorders and health issues. To help balance this system, probiotics have often been administered to both humans and animals, such as livestock. This study aimed to determine what beneficial effects a novel strain of Enterobacter cloacae, strain JD6301, could offer to a host in the presence of an enteric infection with E. coli O157:H7. Upon administration of JD6301, supplemented animals had overall less E. coli present in the …

Growth Implant Timing And Effectiveness In Beef Cattle, Herschel Brad Jones

Theses and Dissertations

One hundred crossbred beef steers were used in two experiments to evaluate effects of growth implants on performance. Steers were implanted either at 3.5 months and weaning (CALF); at weaning (WEAN); or not implanted (NONE). In Experiment 1 implanted steers weighed more at weaning (P = 0.01), and had a higher ADG for the entire trial compared to non-implanted steers (P = 0.03). In Experiment 2, at 80 d post wean, implanted steers outweighed NONE (P = 0.09). Overall ADG (P = 0.01) was greater for cattle that were implanted. In the feedlot the CALF group had the lowest ADG …

Impact Of Early Childhood Classroom Resources On Process Quality Beyond Technical Assistance, Julie Anna Sims

Theses and Dissertations

This study sought to determine whether phase 1 early childhood classrooms receiving classroom resources and technical assistance for early childhood teachers through the Allies for Quality Care project would have greater improvements in process quality than phase 2 classrooms receiving only technical assistance. Process quality refers to what children experience in the early childhood classroom that directly impacts their development and was assessed through the Environment Rating Scales (ERS). Classroom resources were provided to improve the quality of the environment that children experience. The technical assistance was one-on-one to help early childhood teachers understand developmentally appropriate practices. The study examined …

Examining Hepatic Steroid Inactivation And Luteal Function Throughout Bovine Pregnancy, Caitlin G. Hart

Theses and Dissertations

The objective of this study was to examine hepatic steroid inactivation and luteal function throughout bovine gestation. In pregnant beef cows, cytochrome P450 3A activity decreased from mid- to late-gestation, while progesterone concentrations tended to increase from mid- to late-gestation. Uridine diphosphate-glucuronosyltransferase activity per kg of body weight was increased in pregnant vs non-pregnant dairy cows. Total corpus luteum (CL) blood perfusion tended to be increased in pregnant vs non-pregnant dairy cows. Hepatic portal blood flow per kg of body weight was increased in pregnant vs non-pregnant dairy cows. Hepatic steroid inactivating enzyme activity, CL blood perfusion, and portal blood …

The Bioluminescence Heterozygous Genome Assembler, Jared Calvin Price

Theses and Dissertations

High-throughput DNA sequencing technologies are currently revolutionizing the fields of biology and medicine by elucidating the structure and function of the components of life. Modern DNA sequencing machines typically produce relatively short reads of DNA which are then assembled by software in an attempt to produce a representation of the entire genome. Due to the complex structure of all but the smallest genomes, especially the abundant presence of exact or almost exact repeats, all genome assemblers introduce errors into the final sequence and output a relatively large set of contigs instead of full-length chromosomes (a contig is a DNA sequence …

The Effects Of Worksite Health Promotion Programs On Employee Biometric Data, Jamie M. Pratt

Theses and Dissertations

INTRODUCTION: Worksite health promotion programs (WHPP) promote maintenance and changes of health-related behaviors of employees. Some companies opt to contract with a third party provider to implement a WHPP. PURPOSE: This study evaluated the participation rates, availability and use of health coaching, and changes in biometric data over a 2-year time period of employees in 13 companies for whom the WHPP was implemented by Wellness Corporate Solutions (WCS). METHODS: We had 2 years of biometric, health risk appraisal (HRA), or health coaching data on 4,473 employees. The statistical analysis included biometric screening data (percent body fat, body mass index (BMI), …

Microvascular Function In Metabolically Healthy Groups Differing In Bmi And Waist Circumference, Nathan R. Earl

Theses and Dissertations

BACKGROUND: Microvascular dysfunction (MD: impaired performance of blood flow, tissue perfusion, blood pressure, etc.) is one of the earliest stages in the progression of various chronic diseases. OBJECTIVE: The aim of this study was to determine if a difference in microvascular function existed between two metabolically healthy groups that differed in BMI and waist circumference. DESIGN: This study employed a causal comparative design, with two groups: I) normal weight (n =14, BMI 28 kg/m2). METHODS: Microvascular function was assessed by measuring skin blood flow (SkBF) using laser Doppler flowmetry during postocclusive reactive hyperemia (PORH). The area under the SkBF time …

A Randomized Controlled Trial To Study The Effects Of Breakfast On Energy Intake, Physical Activity, And Body Fat In Women, Gabrielle Marie Lecheminant

Theses and Dissertations

PURPOSE: The purpose of this study was to determine the effects of eating breakfast on energy intake, physical activity, body weight, and body fat in women who were nonhabitual breakfast eaters over a one-month period. METHODS: We tested 49 premenopausal, nonhabitual breakfast-eating women to compare the effects of eating breakfast versus not eating breakfast. Each participant was randomized to one of two conditions: breakfast or no breakfast. Breakfast eaters were required to eat within an hour and a half of awakening and had to be finished eating their breakfast meal by 8:30 A.M. Non-breakfast eaters were defined as not consuming …

Role Of Epistasis In Alzheimer's Disease Genetics, Mark T. Ebbert

Theses and Dissertations

Alzheimer's disease is a complex neurodegenerative disease whose basic etiology and genetic structure remains elusive, despite decades of intensive investigation. To date, the significant genetic markers identified have no obvious functional effects, and are unlikely to play a role in Alzheimer's disease etiology, themselves. These markers are likely linked to other genetic variations, rare or common. Regardless of what causal mutations are found, research has demonstrated that no single gene determines Alzheimer's disease development and progression. It is clear that Alzheimer's disease development and progression are based on a set of interactions between genes and environmental variables. This dissertation focuses …

Targeted Sequencing Of Plant Genomes , Mark D. Huynh

Theses and Dissertations

Next-generation sequencing (NGS) has revolutionized the field of genetics by providing a means for fast and relatively affordable sequencing. With the advancement of NGS, whole- genome sequencing (WGS) has become more commonplace. However, sequencing an entire genome is still not cost effective or even beneficial in all cases. In studies that do not require a whole-genome survey, WGS yields lower sequencing depth and sequencing of uninformative loci. Targeted sequencing utilizes the speed and low cost of NGS while providing deeper coverage for desired loci. This thesis applies targeted sequencing to the genomes of two different, non-model plants, Artemisia tridentate (sagebrush) …

Preventing Sleep Deprivation In Shift Nurses: What Is The Best Evidence?, Rhonda D. Wilkins

Theses and Dissertations

Insufficient sleep can lead to a variety of cognitive problems such as impaired learning ability, slow thought processes, slower reaction time, poor mood, risky decision-making, inattention, diminished communication skills, and the danger of falling asleep during critical times. Sleep deprived shift nurses are a risk for making erTors and developing health problems. The purpose of this independent study is to detennine the best evidence to prevent sleep deprivation in shift nurses.

A comprehensive review of the literature was perfonned to detennine best practices of preventing sleep deprivation in shift nurses. Literature searches using CINAHL, Medline, Medline Plus, PubMed, Cochrane Library, …

Effect Of Food Aid Delivery Mode On Food Production In Recipient Nation, Steven Marc Wolf

Theses and Dissertations

The purpose of this study was to provide quantitative evidence linking food aid delivery mode and its correlation with food production in the recipient nation. Debate over the best way to provide food aid to developing countries inspired this study. The three delivery modes used for this study were direct transfer, locally purchase, and triangular purchase. Food aid data was available from The World Food Programme and food production indices were available from The World Bank. Correlations between the independent variable (food aid quantity by delivery mode) and dependent variable (food production index) were examined using the coefficient of determination, …

Effects Of Genetic Modification Of Phosphoinositol Pathway On Light Signaling And Secondary Metabolism In Tomato Plants, Mohammad Alimohammadi

Theses and Dissertations

Inositol- (1,4,5) triphosphate (InsP3) is known as an important signaling factor and a regulator of plant responses to environmental and stress conditions. Overexpression of the InsP 5-ptase gene is found to enhance tolerance of transgenic tomato plants to abiotic stress and the induction of lycopene synthesis in InsP 5-ptase transgenic plants. Here, we demonstrated that the genetic reduction of InsP3 affected the overall phosphoinositol pathway, flavonoid metabolism, and light signaling in InsP 5-ptase transgenic plants. Transgenic plants that overexpress the InsP 5-ptase gene demonstrate an increase in the expression of major light signaling transcription factors (LeHY5, SIMYB12, LeELIP). We found …

The Service Learning Experiences Of Hispanic High School Students On The U.S.-Mexico Border, Andrea Joy Garza

Theses and Dissertations

This phenomenological qualitative study explored the service learning experiences of Hispanic high school students on the U.S.-Mexico border. This study took place in a high school located in south Texas along the U.S.-Mexico border; population approximately 248,142 and ethnically 95.6 percent Hispanic (U.S. Census, 2013). The participants were selected via purposive sampling, thus it was restricted to participants involved in the service learning opportunity with the Volunteer Income Tax Assessor (VITA) program while taking a high school level financial analysis class. Service learning education is defined as the integration of academic material, service activities, and critical reflection based on reciprocal …

The Influence Of Ishraq Program As Non-Formal Education On Girls’ Education In Upper Egypt, Heba Galal Mohamed

Theses and Dissertations

Despite national gains in school enrollment, health, and economic development, adolescent girls in Upper Egypt are facing critical gender gaps in schooling (Isharq Program, 2013). The present research deals with how the Ishraq program, as non- formal education, has succeeded in advocating the education of adolescent girls in Upper Egypt and making their transition into formal schooling. Ishraq is a program running under an international NGO that is designed to address the educational problems in rural areas in Egypt where there are problems with schooling for young girls. Ishraq is a second chance for education that is providing necessary skills, …

Repayment Determinants For Egyptian Microfinance Institutions, Reem Nabil Hashad

Theses and Dissertations

Finance is one of the core development axes and is the basis for job creation, income, poverty reduction and economic growth. Financial resources in Egypt are both idle and misallocated. Access to finance is a major constraint especially to the poor stratum of the Egyptian society. Microfinance is one of the means through which the poor can gain access to finance. However, Egyptian microfinance institutions (MFIs) are inefficient and are unable to supply 90% of the demand for microcredit. This research focuses on how the supply-demand gap of microcredit in Egypt can be reduced. Hence this paper examines what are …

Expression Of Facultative Den Behavior In Ursidae, Nicholas Lee Fowler

Theses and Dissertations

Many ecological and evolutionary processes have been described underlying natural selection but supporting evidence in wild settings is often lacking and our understanding remains incomplete. Using a multi-scale approach, I investigated expression of facultative den behavior (denning) among and within ursid species. Among species, lack of denning behavior was supported by relaxation of selection pressures of seasonal food availability due to realization of two sources of ecological opportunity; colonization of new habitat and adaptation of key innovations. Of denning species, I found evidence of adaptive ecological plasticity in den chronology among and within populations consistent with relaxed selective pressures influencing …

Effect Of Supplementing Heat Stressed Dairy Cows With Electrolytes On Milk Yield, Composition, And Blood Metabolites, Carlos J. Cabrera

Theses and Dissertations

This study was done to examine the effects of supplementing electrolytes to heat stressed transitioning cows on dry matter intake (DMI), milk yield (MY), and blood metabolites. Overall, 104 Holstein and Jersey cows were utilized from August to September, 2012, and from August to November, 2013. Control (CON) cows were fed standard TMR and E+ cows received the same TMR plus 170 g of Bovine BlueLite. The DMI, MY and composition, rectal temperature, and respiration rate were monitored daily; while blood metabolites, body weight, condition score and frame size (withers height, hip height, and heart girth) were measured weekly. The …

Seediness And Sensory Differences Between Highbush, Southern Highbush, And Rabbiteye Blueberries, Amanda Renee Pegg

Theses and Dissertations

Seediness and other differences between rabbiteye-RB (Vaccinium ashei), southern highbush-SHB (Vaccinium darrowii), and commercial market equivalent, highbush-HB (Vaccinium corymbosum) varieties/lines were determined. Forty six selections of rabbiteye and southern highbush blueberries, and three highbush varieties, were analyzed for seediness (seeds per berry volume, SPV). There were no differences (p > 0.05) in SPV between SHB and RB selections, but differences (p < 0.05) existed within species. Berries were assigned to 8 groups based on SPV to conduct sensory panels and chemical analysis. Consumers perceived RB more different than SHB from HB blueberries. In the descriptive panel, differences (p < 0.05) were found in shriveling, skin intactness, grittiness, seediness, and tartness. The soluble solids to acid ratio of HB, SHB, and RB averaged 16.05, 9.60, and 11.05%, respectively. Some consumers and trained panelists can discern differences due to seediness and other traits between blueberry species.