Digital Commons Network^™

Detection Of Neuron Derived Cfdna In Blood Plasma: A New Diagnostic Approach For Neurodegenerative Conditions, Chad Alan Pollard

Theses and Dissertations

Neurodegenerative diseases such as Alzheimer’s disease (AD), Parkinson’s disease (PD), and amyotrophic lateral sclerosis (ALS) are characterized by the progressive loss of distinct neuronal subtypes, yet current diagnostic tools are either invasive, limited in resolution, or poorly suited for early detection and longitudinal monitoring. This dissertation began with a proof-of- concept study using bisulfite-PCR at a neuron-enriched locus to detect cortical neuron-derived cell-free DNA (cfDNA) in AD plasma samples, establishing the potential of cfDNA as a marker of neuronal injury. Building on these preliminary findings, a novel framework was developed utilizing Oxford Nanopore Technologies (ONT) to profile genome-wide native DNA …

The Construction Of Ph- And Temperature-Responsive Drug Delivery Systems Based On Metal Organic Frameworks And Phase Change Materials, Wanying Wei

Theses and Dissertations

In this study, core-shell microparticles were synthesized using coaxial electrospray techniques, with Zeolitic Imidazolate Framework-8 encapsulating either Rhodamine B or Doxorubicin in the core. ZIF-8 is a material well regarded for its effectiveness in drug delivery systems. Phase change materials are composed of a 4:1 mixture of lauric acid and stearic acid in the shell. The PCM chosen has a melting point close to physiological body temperature. The structural morphology and thermal stability of the microparticles were confirmed using Scanning Electron Microscopy, X-ray Diffraction, Differential Scanning Calorimetry, Thermogravimetric Analysis, and Infrared Spectroscopy. The release of the drug was influenced by …

Non-Invasive Near-Field Microwave Sensors For Industrial Internet Of Things Applications, Abirami K

Theses and Dissertations

The industrial sector has been revolutionized by Industrial Revolution 4.0 with the advent of the Internet of Things (IoT) which integrates the physical environment using smart sensors and Artificial Intelligence (AI) mechanisms. This demands the design of smart sensors and actuators to improve the quality of services in Industrial IoT (IIoT). The sensor design using the microwave resonating principle has attracted the research and industrial community for portable, non-invasive, non-destructive and hygienic means to evaluate the quality of substances in the food and farming industry. The microwave planar sensor has advantages in terms of low footprint, low cost, and ease …

Investigating The Ecology Of The Homeless Court: The Promise Of Quasi-Experimental And Statistical Learning Methods, Dylan Wong

Theses and Dissertations

Interactions with the legal system are a crucial factor in maintaining homelessness. Difficulties in addressing legal issues are barriers to accessing services and finding employment and housing. Across the United States, the homeless court is an emerging legal program that has been developed to break this cycle, but they appear to face numerous capacity and coordination problems. Few empirical and systematic investigations have been conducted on homeless court programs. Further, previous studies of homeless courts focus their process and outcome measures on the priorities of the legal system. An investigation into the ecology, or environmental context, of homeless persons would …

Cultural Competency Of Teachers And Its Effect On Their Instructional Practices, Martin Sims

Theses and Dissertations

Core content teachers at S Middle School participate in a study that reveals how increased cultural competency levels among 7^th-grade core content teachers positively influences their instructional practices. The increased cultural competency led to a more inclusive and effective learning environment across all core content areas. Throughout the study, teachers reflected on their own cultural identities while also considering the diverse cultural backgrounds of their students in each class period. As a result, teachers gained a deeper understanding of how cultural factors shape students' learning experiences and needs. The participants adjusted their teaching strategies to better connect with …

Novel Approaches To Combat Neuro-Degradation: Investigations Of Two Supplemental Strategies Aimed At Improving Warfighter Performance, Blaine Lints

Theses and Dissertations

This dissertation examines the efficacy of two distinct nutritional supplementation strategies for improving physical and/or cognitive performance under stress. The first study investigated the effects of 150 mg caffeine plus 150 mg theacrine ingestion in tactically trained individuals. Results indicated that the combination of caffeine and theacrine was associated with significant improvements in reaction time, accuracy, and measures of ocular control following high-intensity interval (HII) exercise. The HII exercise protocol consisted of ten repetitions of 60s high-intensity intervals (≥90% maximal oxygen uptake [VO_2max]) followed by 120s low-intensity intervals (~40% VO_2max) on a treadmill. These benefits were …

Examining The Impact Of Teacher Participation In Pedagogical Content Knowledge Development Intervention On The Academic Achievement Of Culturally And Socioeconomically Diverse Students, Emelyne Viteve Baucicaut

Theses and Dissertations

This action research study examines the impact of a Pedagogical Content Knowledge (PCK) intervention on elementary teachers' instructional practices and student outcomes in Math and English Language Arts (ELA). The goal was to address student underperformance by enhancing teachers' PCK and exploring how improved content knowledge and pedagogical strategies influence classroom teaching and student achievement. Using a concurrent mixed-methods design, baseline data were collected through surveys, classroom observations, and student assessments. Over five weeks, teachers participated in professional development focused on lesson planning, student exemplars, and success criteria. Due to scheduling constraints, computer-based assessments were replaced by analyzing student work …

Camp In The Kitchen: Feminism And Craft In Liza Lou's Beaded Worlds, Aurora X. Bell

Theses and Dissertations

Kitchen (1991–96) was the first major work of art by American artist Liza Lou. It is an installation of a life-size kitchen in which every surface and object is covered in glass beads. Kitchen, which Lou refers to as a monument to women’s unsung domestic labor, received considerable critical attention and drew the artist into ongoing conversations around the relationship between “fine art” and “craft” and about the role of feminism in the art of the 1990s. In contemporary interviews, however, Lou expressed discomfort with the label of “craft” as well as with feminist art that she considered “didactic.” …

Multi-Dimensional Strategies Impacting Beginning Readers’ Word Solving And Engagement, Brooke Clayton

Theses and Dissertations

This dissertation studied the impact of reading instruction on beginner-level readers’ decoding skills and engagement among four first-grade students in Lexington, South Carolina. The following research question directed the study:

Will an instructional strategy foregrounded on direct, systematic phonics instruction in conjunction with multi-dimensional reading strategies improve beginner readers’ decoding skills and engagement?

The study is grounded in several theoretical frameworks, including Scarborough’s Reading Rope (Appendix A), the Simple View of Reading, the Active View of Reading (Appendix C), Onset-Rime phonics instruction, and Self-Determination Theory. Interventions included the LETRS Science of Reading Phonics Small Group Plan (Moats & Tolman, 2019) …

Deprivation And Threat As Predictors Of Executive Functioning And Emotion Regulation In Early Adolescence: The Protective Role Of School Support, Abigail Best De Arellano

Theses and Dissertations

Childhood adversity (CA) impacts a large proportion of US youth with far reaching consequences for development and subsequent health outcomes (McLaughlin et al., 2012). Given the widespread impact of CA, it is imperative to understand how these exposures impact development, as well as identify opportunities for intervention. McLaughlin and Sheridan (2016) proposed the Dimensional Model of Adversity of Psychopathology (DMAP), providing a framework for understanding the heterogeneity of outcomes related to exposure to CA. DMAP emphasizes that deprivation (i.e., lack of expected cognitive or social stimulation) and environmental threat (i.e., exposure to harm or the threat of harm) impacts youth …

Explainable Process Recommendation Through Multi-Contextual Grounding Of Dynamic Multimodal Process Knowledge Graphs, Revathy Venkataramanan

Theses and Dissertations

Can I eat this food or not? Is this food suitable for diabetes and why? Which AI pipeline is best suited for a given task and dataset? How should an end-to-end pipeline be constructed? These questions differ from factual question-answering tasks. Recipes and AI pipelines are processes consisting of several entities interacting with each other. A recipe consists of ingredients, cooking methods, and their interactions, while an AI pipeline includes datasets, preprocessing techniques, models, hyperparameters, tasks, and results. Each entity must be analyzed individually, and collective inferencing is performed to derive the final decision. This decision-making process, known as compositional …

I Belong Here And Am Thriving: Understanding Full-Time Graduate Student Well-Being And Belonging In An Msw Program, Lana Hart Elkins

Theses and Dissertations

Graduate-level social work students face high levels of stress that can impact their academic success and well-being. According to a national college health assessment, fulltime Master of Social Work (MSW) students at a southeastern university report significantly more academic, career-related, and financial stress than peers in other graduate programs. This study addressed the need to better understand full-time MSW student experiences and identify ways the program can mitigate stress, promote wellbeing, and foster a sense of belonging. Using a two-phase qualitative action research design, the study first analyzed responses to 12 open-ended questions from four student feedback surveys. These findings …

Academic Wellness In Health Professional Students: The Intersection Between Dietary Attitudes And Behaviors, Sleep Quality, And Mental Health, Kenya Shereece Moore

Theses and Dissertations

Academic success is strongly influenced by student well-being, with nutrition, sleep, and mental health emerging as critical and interconnected components. Health profession students often face significant mental and physical demands, as they navigate their educational training, which can challenge their ability to maintain healthy behaviors and access necessary support. Although these students are often trained to recognize, treat, and promote wellness in others, they may struggle to prioritize or seek care for themselves. This work aims to explore the health behaviors and care-seeking patterns of health profession students, with a focus on dietary habits, sleep, and barriers to mental health …

The Impact Of Adverse Childhood Experiences And Living With Hiv On Cardiovascular Health And Cardiometabolic Disorders In An African Population: An Analysis Of The Indepth Community In Rural South Africa, Samuel Gavi

Theses and Dissertations

Background: Adverse Childhood Experiences (ACEs) have been shown to be associated with cardiometabolic disorders (CMDs) and poor CVH; however, in Africa, this has not been well studied. Cardiovascular health (CVH) is an important measure of risk for cardiovascular diseases (CVD) developed by the American Heart Association (AHA). With the increasing prevalence of CVD in Africa, CVH is a potential upstream prevention approach. Africa also has the highest prevalence of HIV, and an increasing prevalence of comorbidities associated with it. Various mechanisms have been investigated to explain the relationship between CVD and chronic HIV infection. This study explores the potential relationship …

The Intersection Of Rurality And Wellness: A Multi-Manuscript Investigation Into Rural Well-Being, Lucas Perez

Theses and Dissertations

Rural communities face unique mental health and wellness challenges, including geographic isolation, provider shortages, and stigma surrounding help-seeking. This dissertation explores rural wellness through three interrelated studies, utilizing both qualitative and quantitative methods to examine disparities, perceptions of rurality, and demographic influences on well-being. The first study utilized the Five Factor Wellness Inventory (5F-WEL) to compare wellness scores between rural and non-rural individuals. The second study employed Interpretative Phenomenological Analysis (IPA) to explore rural definitions of wellness, as well as barriers and strengths related to mental health. The third study examined how perceptions of rurality and demographic factors predict wellness …

Exploring The Holistic Wellness Of Black Women Counselors, Shanta A. Jerideau

Theses and Dissertations

Counselor wellness is crucial for clinician longevity and ethical practice. However, there is limited literature on the holistic wellness of Black women counselors. This manuscript probes into the lived experiences of Black women counselors, particularly in relation to the sociopolitical climate and racial injustices in the United States. Utilizing Consensual Qualitative Research, we examine the experiences of 15 Black women counselors working in counselor education, private practice, and community agencies within the Southeast Region of the United States. We anticipate this approach will enhance counselor education literature and foster increased peer support.

Investigating Mechanisms And Day-Level Factors That Influence Children’S 24-Hour Movement Behaviors, Hannah Parker

Theses and Dissertations

Twenty-four hour movement behaviors (i.e., physical activity (PA), screen time (ST), and sleep) are associated with health outcomes, chronic disease (obesity, type 2 diabetes), and mortality. Most literature evaluating children’s 24-hour movement behaviors has focused on static person-level demographic information such as age or biological sex. Relatively less work has examined the variable factors that change day to day, such as environment or context. The Structured Days Hypothesis (SDH) suggests that when children are in structured environments, they have healthier movement behaviors compared to less structured environments where children have more discretionary time to engage in obesogenic behaviors. Besides school/childcare, …

Integration Of Mechanism-Driven Computational Modeling And Public Data Resources For Chemical Toxicity Assessment, Elena Chung

Theses and Dissertations

Regulatory toxicology depends primarily on animal testing, which limits throughput, mechanistic interpretability, and applicability to human-relevant endpoints. This dissertation presents computational approaches that integrate publicly available bioactivity data with machine learning to support chemical safety evaluation. Oral carcinogenicity was modeled using curated chemicals from the US Environmental Protection Agency’s Integrated Risk Information System (IRIS) and activity data from over 1,900 PubChem assays. Quantitative structure-activity relationship (QSAR) models trained on statistically prioritized assays demonstrated strong predictive performance and were applied to rank the carcinogenic potential of more than 16,000 compounds. Hepatotoxicity was evaluated using a hybrid modeling approach that combined structural …

Predicting Single-Leg Eccentric Hamstrings Strength In Aging Athletes Using Diagnostic Ultrasound- And Survey-Derived Parameters, Lee J. Hinkle

Theses and Dissertations

Introduction: Eccentric hamstring strength declines with age and contributes to mobility limitations. Although Nordbord testing quantifies eccentric strength, few studies have examined it in older adults, and none have predicted strength performance on this test using ultrasound or survey- based measures. Methods: We recruited 122 senior athletes (60 female) at the 2024 Huntsman World Senior Games. Ultrasound-derived cross-sectional area (CSA), echogenicity, and subcutaneous fat thickness, along with demographic and activity data, were used to predict single-leg peak force using Bayesian regression. Models were sex-stratified, and predictors were selected using leave-one-out cross-validation (LOO IC). Results: In males, CSA, age, leg side, …

The Dubr/Mir-210-5p/Retsat Axis In Cholesterol-Associated Steatohepatitis: Clinical Signatures And Molecular Mechanisms, Hana Mahmoud Abdelzaher

Theses and Dissertations

Metabolic-associated fatty liver disease (MAFLD) represents an increasing global health challenge, with cholesterol-associated steatohepatitis (CASH) emerging as a distinct pathological entity warranting investigation. While CASH demonstrates unique features compared to traditional triglyceride-driven steatohepatitis, its underlying molecular mechanisms remain poorly understood. This study investigates a novel regulatory axis involving DPPA2 Upstream Binding RNA (DUBR), miR-210-5p, and retinol saturase (RETSAT). 170 patient samples revealed progressive axis dysregulation in disease states, with RETSAT emerging as a highly accurate diagnostic marker (AUC=1.000). Using novel in vitro models of MASH and CASH, we demonstrated that miR-210-5p modulation significantly affects lipid accumulation and cellular phenotype, with …

Building Brains And Relationships Beginning From Birth: Exploring Multilingual Parents' Experiences With A Babies Initiative Family-School Partnership, Diane D. Joyce

Theses and Dissertations

This study examined multilingual families’ experiences in a family-school early literacy program during the critical period of brain development, the Phlournoy Babies Initiative (PBI). Participants included five multilingual (ML) and immigrant families who have participated in this program for multiple years. In addition, this study probed how families were applying the strategies shared during PBI sessions within the context of their culture and home environment. Sociocultural, ecological systems, and culturally responsive teaching theories formed the framework of this study. A narrative inquiry approach was applied, which provided the researcher the opportunity to share the stories and experiences of the participants. …

University Counseling Center Therapists' Perspectives Of Counseling About Nutrition, Ana Lucia Pacheco

Theses and Dissertations

This study examined university counseling center therapists' perspectives on discussing nutrition in therapy. Despite the established link between nutrition and mental health, minimal research has explored how therapists approach these discussions. This study used interpretative phenomenological analysis (IPA) to analyze semi-structured interviews with 10 university counseling center therapists from the Mountain West region. Four overarching themes emerged: a deficit view of nutrition, perceived utility, ethical practice, and holistic care. Therapists unanimously recognized the connection between nutrition and mental health and primarily discussed nutrition with clients presenting with eating disorders or disordered eating, those who express interest, or as part of …

Assessing Illegal Take Of Sea Turtle In Bocas Del Toro, Panamanian Caribbean, Daniela Carolina Rojas-Cañizales

Theses and Dissertations

In Bocas del Toro, Panama, sea turtles were exploited for generations to supply local, national, and international markets. Today, sea turtle species are globally protected by different laws, and Panama is no exception. However, the use of sea turtles in Bocas del Toro and local perceptions regarding the consumption of these reptiles are unknown. Accordingly, during May of 2024, I conducted twenty-five semi-structured interviews with key informants selected by targeted and snowball sampling. Sea turtle consumption persists, and there is a clandestine network to sell turtle meat and derived products in Bocas del Toro. This study provides the first assessment …

The Role Of Cephalic Mechanosensory Bristles In Flight Initiation Of The Oleander Hawkmoth, Daphnis Nerii, Maitri Manjunath

Theses and Dissertations

During flight, insects sense air movement past their bodies via sensory bristles distributed all over their body. These bristles provide mechanosensory feedback to their nervous system, thereby modulating wing motion to cope with ambient wind conditions. In many insect systems including locusts, moths, etc., flight can be rapidly initiated by stimulating a set of bristles called cephalic bristles on their head. Rapid initiation of flight requires diverse, distributed flight-related reflexes to be simultaneously activated.

Thus, we hypothesized that cephalic bristles directly and simultaneously trigger diverse flight-related reflexes. We tested this hypothesis using the Oleander hawkmoth, Daphnis nerii, as our study …

Perceptions Of Faculty On Trauma-Informed Practices At The American University In Cairo, Marwa M. Emara

Theses and Dissertations

Abstract

Trauma-informed education is gaining momentum in higher education as a framework that seeks to create learning environments that are safe, inclusive, and responsive to the emotional and psychological needs of students—particularly those who have experienced trauma. This qualitative study, situated at the American University in Cairo (AUC), explores how faculty perceive, understand, and implement trauma-informed practices in a diverse university context that includes refugee and displaced students. Data were collected through semi-structured interviews with seventeen faculty members from various disciplines and incorporating an autoethnographic reflection. An expert interview was conducted with Dr. Mays Imad, a leading scholar in trauma-informed …

Evaluating Simulated Threecornered Alfalfa Hopper (Hemiptera: Membracidae) Damage In Cotton (Malvales: Malvaceae) And Identifying Conducive Environments, Reece Starnes Butler

Theses and Dissertations

This research evaluated the effects of simulated threecornered alfalfa hopper (TCAH), Spissistilus festinus (Say), damage in cotton and aimed to identify environments that may contribute to an increase in TCAH injury. During the 2023 and 2024 growing season, simulated TCAH damage was imposed at 0%, 10%, 20%, 30%, 40%, and 50% at the three and six leaf growth stage. Agronomic data included plant heights, node counts, and plant mapping, which was collected throughout the growing season, while lint yield was recorded at harvest. Damaged plants exhibited reduced plant heights and node counts, with an increase in nodes above cracked boll …

Evaluating The Efficacy Of Functional Feed Additives In Broilers With Or Without A Live Coccidiosis Vaccine Challenge, Dalton Gerard Dennehy

Theses and Dissertations

Functional feed additives, such as probiotics, prebiotics, and phytogenics, have seen increased usage in commercial poultry production due to their ability to improve growth performance, meat yield, and overall health status. Therefore, the objective of this research was to evaluate the efficacy of functional feed additives in broilers with or without an enteric challenge induced via a live coccidiosis vaccine (LCV). In the first study (Chapter II), broilers were raised on used litter without an LCV challenge and fed varying levels of a multi-strain Bacillus-based probiotic in the starter phase (1x or 2x dose). Reduced performance was observed in early …

Estimation Of Soybean Root Traits Using Computer Vision And Deep Learning, Muhammad Usman

Theses and Dissertations

Root phenotyping provides insights into root structure, function, and response to environmental stimuli, aiding researchers in improving crop yields, developing new plant varieties, and enhancing plant health. Traditionally, measuring root traits, such as length and area, is manual, time-consuming, and resource intensive. This study introduces a deep learning-based computer vision method for soybean root segmentation and traits estimation using RGB (red, green, blue) images captured under controlled illumination. A separate web-based application was also developed that can extract the root length and area using RGB images. A diverse set of 3,363 soybean root images was collected using an in-house root …

Combined Effect Of Chitosan And Lemon Essential Oil-Infused Slush Ice On The Shelf Life Of Channel Catfish, Dianna Wilson

Theses and Dissertations

The objective of this research was to investigate the biopreservative effects of chitosan and lemon essential oil in slush ice treatments on the microbial shelf life of refrigerated catfish fillets. Catfish fillets were stored in slush ice treatments containing chitosan (CH), lemon essential oil (LO), a combination (CH+LO), water (CTRL), and no slush (NS) for three d at 4 C, then stored at 4C for twelve d post-slush. The growth of background microflora in catfish fillets treated with CH and CH+LO was reduced (P<0.05), maintaining total aerobic counts below 4.5 log CFU/g, while the control reached spoilage levels (>7 log CFU/g) by Day 6. Chitosan effectively extended the microbial shelf life but lowered pH, …

Effects Of Different Forms Of Dietary Zinc On Weaned Steers When Fed Prior To Weaning, Christopher Lane Addison

Theses and Dissertations

Zinc (Zn) is crucial for many life sustaining processes within the body. Additional Zn is known to be beneficial during certain periods of increased stress. Weaning is one of the most stressful periods of beef calves’ lives. Due to this it was hypothesized that additional zinc in an organic form would increase weight gain and immune response compared to additional zinc in an inorganic form or no added zinc. No differences were found for ADG, Th score, IgG concentration, or mineral concentration among treatments. However, there was a novel and unexpected finding when there was no difference found in preweaning …