Digital Commons Network^™

Island Nation Duress: Simulating Passive Peer-To-Peer Bluetooth Communication During Disaster Relief, Jason K. Medeiros

Theses and Dissertations

Pacific Islands under U.S. jurisdiction are highly vulnerable to natural disasters, yet many lack the infrastructure to effectively respond and recover. Clear communication during and after such events is critical for evacuation, hazard awareness, and first responders’ coordination. This research explores a simulation-based approach using Bluetooth communication to relay messages across Guam, assessing its efficiency through statistical analysis. By examining regional differences and geographic impacts on Bluetooth messaging, the study aims to identify key factors that enhance peer-to-peer communication for timely and effective disaster response.

Integrating Planetary Health Into Public Health Education For Sustainable Healthcare Practices, Eman Aboualy

Theses and Dissertations

Introduction: Integrating planetary health in public health education became an urgent global call for action since the WHO announced that the climate change and environmental degradation are the most threat facing the humanity in the recent decades (1). According to that, many countries are developing strategies for counteracting and mitigating the impact of climate change and restore the planetary health. These strategies are including policy drafting and preparing their public health professionals to recognize and address the health effects of environmental change, promote practices for sustainable healthcare. Although Egypt is formulating different policies to cope with and respond to the …

Identification Of Novel Argonaute Proteins Using A Metagenomic Mining Approach, Lobna Abdallah Ghonaim

Theses and Dissertations

Gene editing is one of the most promising tools in science. It enables precise modifications of an organism's genetic material. Metagenomics is considered a powerful tool that unlocks the broad genetic potential found in uncultured microbial communities. Exploring the genetic diversity of uncultured microbial communities helps identify novel functional proteins with unique properties and make the best use of these diverse microbial ecosystems.

We developed and employed a metagenomic-based approach to mine more than 1000 metagenomes for prokaryotic argonaute proteins (pAgos), a potential gene editing machinery encoded in bacterial and archaeal genomes. Our workflow involved strict quality control, sequence assembly, …

The Role Of Gsk-3Α Inhibition In Attenuating Lps-Induced Inflammation In Raw 264.7 Cells, Oluwaseyi Oyewole

Theses and Dissertations

Glycogen synthase kinase-3 (GSK-3), an enzyme that modulates glucose metabolism, has been recognized as an essential target in major inflammatory diseases based on its great specificity in substrate recognition. GSK-3β has been connected to cancer, obesity, liver diseases, and neurological diseases. Nonetheless, the other GSK-3 isoform, GSK-3α, is much less studied. Previous studies from our laboratory showed that inhibiting GSK-3α potentially confers an anti-inflammatory response when tested on microglia. However, little has been documented regarding the exact mechanism by which GSK-3α exerts its effect. One of the potential mechanisms involving its anti-inflammatory effect could be partially attributed to activating the …

Functional Dissection Of The Periplasmic Domain Of Rcsd, The Phosphotransfer Protein Of Escherichia Coli Rcs System, Abdelraouf Dapour

Theses and Dissertations

Microbial resistance to antibiotics poses a major health threat worldwide, prompting the scientific community to dedicate considerable efforts toward understanding the molecular mechanisms underlying this resistance. The Gram-negative cell envelope serves as a formidable barrier to environmental stressors and numerous antimicrobials. In E. coli and other enterobacteria, the Rcs system (Regulator of capsule synthesis) is a crucial two-component signalling pathway that plays a critical role in maintaining the integrity of the bacterial envelope. One unique aspect of the Rcs system is the involvement of several auxiliary proteins in its signalling cascade. Among them, RcsD, an inner membrane 100 kDa protein, …

The Role Of Biofilm Matrix On The Growth, Survival, And Diagnosis In Enterobacteriaceae, Kavi Bharathi R

Theses and Dissertations

Bacteria in nature live together encased in a matrix creating community ecosystems called biofilms, this structured space protects them from hostile environments. This study aimed to understand the role of the biofilm matrix in the growth and survival of Enterobacteriaceae. Our hypothesis proposes that subgroups of individuals within a biofilm can specialize in the production of different matrix components.

Matrix expression is metabolically costly, despite its unicellular simplicity, microbes can coordinate complex behaviour like sharing of matrix between producers and non-producers as an adept evolutionary strategy. Their resilience to stress during biofilm formation was studied to assess their abilities to …

Assessment Of Risk Factor Prediction Using Machine Learning Techniques And Hybrid Approach Based On Soft Sets, Menaga A

Theses and Dissertations

Cardiovascular diseases (CVDs) are the leading cause of mortality worldwide, and India reports a significantly high death rate due to its large population base and the increasing prevalence of non-communicable diseases. National statistics indicate that 20–27% of deaths in India are attributed to CVDs, with the proportion steadily rising over the years. Recognizing the urgency of early detection and risk prevention, the World Health Organization (WHO) introduced “The Global Action Plan for the Prevention and Control of Non-Communicable Diseases (2013–2020),” emphasizing early identification, risk reduction, and timely treatment. In this context, decision-making applications have gained importance across domains especially healthcare …

Customized Feminine Hygiene Wash Containing Postbiotics From Lactobacillus Spp. To Treat Urinary Tract Infections (Uti), Veena G. Nair

Theses and Dissertations

Probiotics, which support host health by maintaining normal microbial flora, offer a promising approach for preventing recurrent infections. To evaluate the prophylactic potential of vaginal probiotics against urinary tract infections (UTIs), we conducted a study on 54 healthy South Indian women aged 18–40 who visited the Obstetrics and Gynaecology outpatient clinic at Trichy SRM Medical College and Research Centre between June and July 2022. Vaginal swabs were collected following ethical guidelines, and commensal microbiota were isolated for probiotic characterization, with the broader objective of developing a customized feminine hygiene wash enriched with vaginal postbiotics.

Lactobacilli cell-free supernatant (CFS) demonstrated potent …

Optimizing Biofilm Assay Reproducibility And Characterizing The 12-Hydroxystearic Acid On Escherichia Coli, Khilola Khusanova

Theses and Dissertations

Biofilm formation in Escherichia coli plays a critical role in microbial chemical communication and surface-associated persistence. This study investigates the antibiofilm activity of 12-hydroxystearic acid (12-HSA), evaluating its R and S enantiomers as well as a racemic mixture. E. coli BL21, biofilms were grown under standardized laboratory conditions and quantified using the Crystal Violet staining assay. Treatment with 12-HSA resulted in a measurable reduction in biofilm biomass, with differences observed between the individual enantiomers and the mixture. These findings suggest that 12-HSA influences early adhesion and biofilm development, supporting its potential as a small-molecule modulator of bacterial surface behavior. This …

School Mental Health Partnerships: A Case Study, Rebecca Bilski

Theses and Dissertations

Education in the United States continues to evolve in response to technological advancements, such as the widespread use of the iPhone, and societal changes in a post-COVID-19 world. Youth mental health challenges are at an all-time high, impacting students’ ability to succeed academically and socially (CDC, 2023). Schools are in a unique position to address these challenges, yet untreated or unidentified mental health issues can hinder students’ progress (Sanchez et al., 2018). This study examines the potential of school mental health partnerships to provide educators with the tools, training, and resources necessary to support students with diverse and complex mental …

Hispanic Paradox In Covid-19: Protective Effects Of Ethnic Density, Gregory Alan Urena

Theses and Dissertations

The COVID-19 pandemic exposed racial and ethnic health disparities in the U.S., with Hispanic populations experiencing higher infection and population-mortality rates than non-Hispanic Whites (NHW). Case fatality rates are closely tied to medical comorbidities such as hypertension, diabetes, and chronic kidney disease, which are more prevalent among Hispanic individuals. Case-fatality rates showed mixed results. Some studies found no differences, while others reported better outcomes for Hispanics, aligning with the Hispanic Paradox, which suggests that despite socioeconomic disadvantages, Hispanics experience equal or better health outcomes. These effects may be tied to the immigrant health advantage, which suggests immigrants tend to be …

Mctr3 Mitigates Cigarette Smoke-Induced Pulmonary Inflammation And Damage, Yu Par Aung Myo

Theses and Dissertations

Smoking is deleterious to our lungs and can precipitate in a multitude of diseases, including chronic obstructive pulmonary disease (COPD). COPD is primarily caused by smoking cigarettes and is a major public health issue. Current therapies only treat symptoms without halting or reversing disease progression, thus highlighting a large unmet need to develop new therapies. We address this gap by investigating maresin conjugate in tissue regeneration 3 (MCTR3) which is a newly discovered specialized pro-resoling mediator (SPM) that has the novel property of promoting tissue repair and regeneration in addition to being anti-inflammatory and pro-resolving. For the first time, we …

Functional Analysis Of Ligand Binding Domain 2 Of Outer Surface Protein C Of Borreliella Burgdorferi, Gavin Z. Chambers

Theses and Dissertations

Lyme disease, caused by the spirochete Borreliella burgdorferi, is the most common vector-borne infection in North America. A critical determinant of early mammalian infection is outer surface protein C (OspC), a highly variable lipoprotein that is indispensable for spirochete survival but whose precise function remains unresolved. OspC has been proposed to mediate diverse roles, including complement evasion, adhesion, tissue dissemination, and antiphagocytic activity, supporting the view that it is a multifunctional virulence factor. Structural studies have identified two putative ligand-binding domains (LBD1 and LBD2), with LBD2 forming a solvent-exposed crown at the membrane-distal end of the OspC dimer.

To …

Improving Telehealth Education And Training: A Needs Assessment Of Healthcare Providers And Trainees, Conor Duffy

Theses and Dissertations

Telehealth utilization has increased dramatically since the start of the COVID-19 pandemic. There are many positive impacts of this shift to telehealth, including increasing access to care and reducing patient burden. However, healthcare providers report several consistent challenges that limit their ability to deliver telehealth, such as effective communication, technological difficulties, and difficulty performing physical examinations. To address these concerns, it is necessary to integrate telehealth education and training into healthcare professional training programs and healthcare systems. While many institutions have implemented telehealth education and training since the start of the pandemic, there is little consistency in the content, duration, …

Birthing While Black: The Color Of Care And Black Women’S Lived Experiences Giving Birth In The United States, Portia Cowlings

Theses and Dissertations

Black women in the United States face disproportionate maternal health outcomes during pregnancy, childbirth, and postpartum compared to other racial and ethnic groups. Despite advancements in healthcare, the United States continues to report the highest maternal mortality rates among high-income developed nations, with Black women experiencing rates up to three times higher than their White counterparts. This phenomenological study explores the lived experiences of Black women in the United States to understand how they navigate the continuum of maternal healthcare, from preconception through postpartum. Guided by the social determinants of health (SDOH) theoretical framework, childbirth was examined through four interconnected …

Larval Environments Affect The Implementation Of The Sterile Insect Technique On Aedes Mosquitoes, Katherine G. Evans

Theses and Dissertations

Sterile Insect Technique (SIT) is an effective method for controlling pests and has been increasing in popularity to control Aedes mosquitoes. SIT involves inundating an area with sterilized male mosquitoes to reduce egg hatch and ultimately the number of wild adults. Previous research indicates that enacting mortality early in the larval life of Aedes mosquitoes can result in counter-productive effects, due to reduced competition among developing larvae. I hypothesize that the effectiveness of SIT at reducing adult populations is modified by densitydependent mortality and density-dependent growth among larvae. I performed two field-to-lab studies and developed an individual-based model to test …

A Novel Silver-Ruthenium-Based Antimicrobial Kills Gram-Negative Bacteria Through Oxidative Stress-Induced Macromolecular Damage, Patrick O. Tawiah

Theses and Dissertations

The rise in drug-resistant bacteria, together with the decline in antibiotic development, requires new strategies for infectious disease control. Gram-negative pathogens are particularly challenging to combat due to their outer membrane. This study highlights the effectiveness of the silver-containing antimicrobial AGXX® against the Gram-negative bacterium Escherichia coli. AGXX® is a promising silver coating that presumably kills bacteria through the generation of reactive oxygen species (ROS). However, neither AGXX®’s mode of action is fully understood, nor have its effects on Gram-negative bacteria or bacterial response and defense mechanisms towards AGXX® been studied in detail. Here, I report that the bactericidal …

Flavorful Foes: Assessing Students’ Perceptions Of Processed And Fast Food And Its Health Implications., Soumi Roy Banerjee

Theses and Dissertations

High fast-food consumption is a common public health concern due to its link to a number of non-communicable diseases and mental health, but increased consumption of processed food, rapid urbanization, and changing lifestyles have led to a shift in dietary patterns. People are consuming more foods high in energy fats and free sugars. This mixed methods study was conducted at a college campus in Georgia, to assess knowledge and understanding of the students between the ages of 18 and 22 years about highly processed Fast Food, and its effects on health. Forty student participants were chosen through convenience sampling and …

Virulence Of Emerging Tick-Borne Pathogens, Borrelia Miyamotoi And Borrelia Mayonii, Dawn Cleveland

Theses and Dissertations

As climate change leads to global expansion of tick habitats, tick borne diseases are becoming more prevalent and widespread, and emerging tickborne pathogens Borrelia miyamotoi and Borrelia mayonii warrant increased study. Both B. miyamotoi and B. mayonii are vectored by the same tick species as B. burgdorferi, the causative agent of Lyme disease, with B. miyamotoi causing a relapsing fever-like disease known as Borrelia miyamotoi disease (BMD), and B. mayonii causing a Lyme disease-like illness with atypical clinical presentations. This dissertation has two aims. The first aim encompasses B. miyamotoi infection and growth, isolation of clonal populations, and characterization of …

Asymmetric Synthesis Of The Hiv Protease Inhibitor Tmc-126 And An Anti-Malarial Agent Via A Titanium Tetrachloride Mediated Asymmetric Glycolate Aldol Addition Reaction. Redefining The Curtius Rearrangement Reaction Via Dehomologation Of Carboxylic Acids Bearing Alpha-Leaving Groups, Kweku Amaning Affram

Theses and Dissertations

Acquired Immune Deficiency Syndrome (AIDS) and malaria are two devastating infectious diseases caused by HIV and Plasmodium parasites. AIDS is caused by HIV-1 and HIV-2, transmitted through sexual contact, blood transfusions, needle sharing, and perinatal routes. Malaria, caused by plasmodium parasites through mosquito bites. Today, millions of people around the world have been infected with HIV and malaria and much research has been done to treat it. However, despite advancements in therapeutic options, these diseases remain a significant global health threat, particularly in developing countries due to the emergence of drug-resistant strains for both HIV and malaria complicating eradication efforts. …

Genetic Epidemiology Of Chronic Wasting Disease And Epizootic Hemorrhagic Disease In North Dakota Deer, Ethan S. Baker

Theses and Dissertations

Wildlife diseases have become a critical factor in the management of animal populations across the globe. Such diseases can compromise individual health, reduce population sizes, and disrupt ecosystem biodiversity. As a result, there can be a loss in recreational opportunities which, in turn, can cause economic losses for communities relying on these populations. Effective wildlife disease management not only requires the ability to respond to outbreaks but also the capacity to predict how diseases interact with populations and ecosystems. Due to the complexity of disease dynamics, a multidisciplinary approach is essential for gaining a comprehensive understanding of these systems. In …

Caspases Protect Against Hyperglycemia Induced Necroptosis-Like Cell Death In Brain Endothelial Cells, Aleena S. Mathew

Theses and Dissertations

Acute diabetes characterized by high spikes in blood glucose levels in association with severe hyperglycemia can lead to an increased risk of ischemic stroke in the brain. To better understand high blood glucose levels, hyperglycemia, in the brain we explored its effects on endothelial cells. Endothelial cells line the inner walls of blood vessels and are directly impacted in hyperglycemia. Brain endothelial cells are a major component of the blood brain barrier (BBB) which tightly regulates the brain’s microenvironment. The type of cell death such as apoptosis, caspase dependent, or necroptosis, caspase independent, can exacerbate diabetes associated complications. There is …

Taking It To The Streets: A Comparative Case Study Of Urban Street Medicine Programs For The Unhoused Population, Dolly Dimple Harris

Theses and Dissertations

“Taking it to the Streets” originated from the 1960s social and political activism (Doohan & Mishori, 2019). The term reflects the 1990s movement when a Philadelphia physician decided that caring for the unhoused population was necessary and started street medicine. Street medicine is an alternative medical approach to formal healthcare intended to address unhoused individuals in their lived environment. In the street medicine model, healthcare providers offer medical support for the unhoused in alleyways, parks, under bridges, and encampments. The purpose of this comparative case study was to examine two street medicine programs’ administrative processes and how they provide healthcare …

Mathematical Modeling Of Cyber-Physical Attacks In Iot Systems: An Epidemiological Approach, Tunde Alesinloye

Theses and Dissertations

The Internet of Things (IoT) has made electronic devices more interconnected, which has increased cybersecurity threats while also bringing previously unimaginable convenience. In this study, we explore the dynamics of malware transmission in IoT systems using a mathematical modeling methodology that draws inspiration from epidemiological approaches. To better characterize contemporary cyber-defense tactics like honeypots and proactive device hardening, we specifically suggest the SEIR-DX model, which expands the traditional SEIR framework by adding two extra compartments: Deceptive (D) and Secured (X).

The transitions between the six compartments Susceptible, Exposed, Infected, Recovered, Deceptive, and Secured are described by a system of nonlinear …

The Influence Of The Covid-19 Pandemic On Student Engagement At A Historically Black University, John C. Travis

Theses and Dissertations

This study examined student engagement across three NSSE administrations at an HBCU—pre-COVID (2018), during COVID (2019), and post-COVID (2022). Engagement was analyzed across four themes: Academic Challenge, Learning with Peers, Experiences with Faculty, and Campus Environment. Results showed that Learning with Peers declined significantly over time, with the largest drop between 2018 and 2022, reflecting pandemic-related disruptions to peer interaction. No significant year effects emerged for the other themes. Two-way MANOVAs indicated that gender, first-generation status, and major did not significantly predict engagement, nor did they interact with year. In contrast, classification emerged as the most consistent predictor, with upper-division …

The Roles Of Readily Releasable Stores Of Endolysosome Iron In The Pathogenesis And Treatment Of Alzheimer's Disease, Darius N.K Quansah

Theses and Dissertations

Alzheimer’s disease (AD) is a neurodegenerative disorder characterized clinically as progressive decline in cognition and dementia. Pathologically, AD is characterized by the extracellular presence of senile plaques composed of accumulated amyloid beta (Aβ) proteins and intraneuronal accumulation of neurofibrillary tangles composed of hyperphosphorylated tau proteins. In addition to these pathological hallmarks, AD pathogenesis is characterized by increases in intracellular iron and reactive oxygen species (ROS) and the link between the two have been well described by Fenton’s chemistry. Endolysosomes appear to be affected very early in the onset of AD. Endolysosomes contain labile stores of iron which can be readily …

Ecological Dynamics In Beaver-Engineered Boreal Wetlands: Amphibian Phenology, Occupancy, And Aquatic Macroinvertebrate Communities, Talon Stammen

Theses and Dissertations

Boreal wetlands are ecologically rich yet vulnerable ecosystems shaped by hydrological dynamics, cold climate regimes, and landscape-scale disturbances. Among the most influential agents of change in these systems are beavers (Castor canadensis), whose engineering activities create a mosaic of lentic habitats at varying stages of ecological succession. This dissertation investigates the structure and dynamics of amphibian and aquatic macroinvertebrate communities across a beaver-influenced wetland landscape in Voyageurs National Park, Minnesota, USA.The first study (Chapter II) examines breeding phenology and detection patterns of boreal anurans using a combination of passive acoustic monitoring and visual encounter surveys at 25 wetlands over four …

Zaasijiwan: An Indigenized Health Communication Framework For American Indian And Alaska Native Communities, Jennifer Wolf

Theses and Dissertations

The Zaasijiwan Framework represents an Indigenized health communication model developed to enhance the cultural relevance, trust, and effectiveness of public health messaging for American Indian and Alaska Native (AI/AN) communities. This qualitative study integrated Indigenous and Western public health knowledge to inform the development of the framework. A comprehensive literature review and two rounds of key informant interviews with health communication professionals informed the design of the framework. Findings emphasized the importance of community co-creation and strengths-based narratives, along with other framework elements, in overcoming medical mistrust and improving health outcomes. The final framework offers public health professionals, Tribal, and …

Ai: A Path Forward In Education - Teachers’ Insights On Implementation, Challenges, And Future Innovations, Mercedes Cordoba Meeks

Theses and Dissertations

Artificial intelligence (AI) is a global phenomenon permeating the fabric of society and transforming the course of culture and commerce (Kishor et al., 2023; Moore, 2024). A review of the extant literature indicates that while many industries have seen significant innovations due to the infusion of AI, the field of education has witnessed a technological evolution inspired by AI-based tools and strategies (Bessen, 2018; W. M. Lim et al., 2023). Artificial Intelligence in Education (AIED) has a history that parallels the development of AI. AIED has shown steady growth and influence in educational programs around the world, with the growth …

Between "Hippies In Suits" And "Money Is The Name Of The Game": Farm Animal Advocates' Analyses Of Political Power In The Farm Bill, Jeff Thomas

Theses and Dissertations

The animal agriculture industry annually slaughters roughly ten billion land animals in the United States. What can be done to improve their welfare and lessen their suffering?

Over the past decade, the problem of farm animal welfare and suffering has drawn increasing interest from the effective altruism community, a loose movement of utilitarian advocates seeking technocratic solutions to social problems (Singer, 2015). As effective altruism has become a more potent driver of the animal welfare movement, an increasing number of nonprofits have begun engaging in political advocacy for farm animal welfare. Facebook co-founder Dustin Moskovitz’ Open Philanthropy is the largest …