Digital Commons Network^™

Adaptations In Orthodontics For Current And Future Covid-19 Best Practices, Jordan Lamb

Theses and Dissertations

Purpose: To determine what adaptations orthodontists made in their practices during the COVID-19 pandemic in response to safety recommendations and to determine which of these adaptations orthodontists plan to continue to implement after resolution of the COVID-19 pandemic.

Methods: An original 34-question survey was sent by mail to a randomized and geographically proportionate selection of actively practicing orthodontic specialists (N = 1000). Questions asked orthodontists about the changes they made during the pandemic in four overarching categories (infection control, social distancing, appliance use, and tele-orthodontics) and whether they anticipated keeping those changes post-pandemic. The data were compared between categorical responses …

The Effect Of Er:Yag, Nd:Yag And Co2 Laser Combined With Hydrogen Peroxide, Sodium Hypochlorite, Chlorohexidine Or Fluoride On Reducing Oral Bacterial Count Implicated In Root Caries, Nitya Reddy

Theses and Dissertations

Purpose: Lasers have been used for treatment of dentinal hypersensitivity and for various bacterial reduction indications in periodontology. Their effectiveness in killing oral bacteria is not well known. The compounding effect of the combination of a laser treatment and adjunct antimicrobial agent use on bacterial viability is still evolving. The purpose of this in vitro study was to evaluate the effect of three lasers commonly used in dentistry in conjunction with chlorhexidine (CHX), hydrogen peroxide (H₂O₂), sodium hypochlorite (NaOCl), or sodium fluoride (NaF) on viability of oral bacteria associated with root caries.

Methods: Three …

Progress Towards Next Generation Modulators Of Ctbp's Oncogenic Effects, Jacqueline L. West

Theses and Dissertations

Human C-terminal binding proteins (CtBP1 and CtBP2) are transcriptional coregulators of multiple genes in the human genome, including tumor suppressor genes (e.g., Bik, PTEN, BRCA1, and E-cadherin) as well as oncogenes (e.g. MDR1 and Tiam1). Both homologues of CtBP are overexpressed in many types of cancer, including breast cancer (92%), ovarian cancer (83%), colorectal cancer (64%), hepatocellular carcinoma (60%), gastric cancer, prostate cancer, and pancreatic adenocarcinoma. Further, expression levels of CtBP correlate with worse prognostic outcomes and more aggressive tumor features because it promotes proliferation, epithelial-mesenchymal transition, and cancer stem cell self-renewal activity.

Our laboratory has identified a lead inhibitor …

The Architecture And Dynamics Of Gene Regulatory Networks Directing Cell-Fate Choice During Murine Hematopoiesis, Joanna Handzlik

Theses and Dissertations

Mammals produce hundreds of billions of new blood cells every day througha process known as hematopoiesis. Hematopoiesis starts with stem cells that develop into all the different types of cells found in blood by changing their genome-wide gene expression. The remodeling of genome-wide gene expression can be primarily attributed to a special class of proteins called transcription factors (TFs) that can activate or repress other genes, including genes encoding TFs. TFs and their targets therefore form recurrent networks called gene regulatory networks (GRNs). GRNs are crucial during physiological developmental processes, such as hematopoiesis, while abnormalities in the regulatory interactions of …

Multinomial Logistic And Negative Binomial Regressions Of Campus Instructional Modes, Institutional Characteristics, And Covid-19 Case Counts In Fall 2020 In The Midwest, Katherine Marian Tyler

Theses and Dissertations

In spring of 2020, a global pandemic shifted American institutions of higher education into a crisis with unprecedented unknown information, guidelines that changed continuously, and impacted the personal and professional lives of students, faculty and staff. This study examined the relationships (1) between campus size, geographic setting, locus of control in Midwest and Mountain American higher educational institutions and their instructional mode in fall 2020, and (2) between those institutional characteristics and the number of reported campus COVID-19 cases in the fall of 2020. Using a multinomial logistic regression and a negative binomial regression with an estimated parameter dispersion, the …

Translocation Of Yops By Yersinia Pestis Into Canine Cell Lines, Betsy Louise Young

Theses and Dissertations

Yersinia pestis, the causative agent of plague, secretes a set of virulence proteins called Yops when in contact with eukaryotic cells. A subset of these Yops is translocated directly into the cytosol of the host eukaryotic cells by the type III secretion system. Using a protein tag-based reporter system, this study is focused on the translocation of these Yops into the cytosol of canine derived cell lines: MDCK and DH82. The MDCK cell line is a canine derived epithelial cell line, and the DH82 cell line is a canine derived monocyte cell line. The reporter system uses a phosphorylatable peptide …

Examining Cultural Identification And Substance Use Among American Indian And Caucasian Adults, Lynn Ruth Martell

Theses and Dissertations

Previous research suggests American Indians (AIs) demonstrate higher levels of substance use and related consequences. AIs have experienced historical trauma through colonization which has created shifts in the way AIs conceptualize their identity. However, the literature on cultural identification as an influencing factor of substance use among AIs is inconsistent. The current study examined alcohol and drug use/related consequences, collective self-esteem, and cultural identification among 213 AI (n = 44) and Caucasian (CA; n = 169) adults via online surveys. Results indicated higher scores on the American Indian Cultural Identification (AICI) scale was a predictor for increased alcohol and drug …

Preparing For Professional Programs: Strategies And Practices For Pre-Medical Education, April Elayne Curry

Theses and Dissertations

This study examines the preparation process for students in professional programs and highlights strategies and practices for pre-medical education. The COVID-19 pandemic has highlighted elements of the healthcare system that explore how critical physicians are that can lead with both a global perspective and with compassionate patient care. In order to understand the competencies required for this leadership process, it is critical to consider the medical school lifecycle from the undergraduate education to residency. Working from the admissions process, we consider what competencies are required for medical school success and physician preparedness and we use that to shape a solid …

Understanding The Underserved Student: Barriers Experienced By At-Risk Students During The Covid-19 Pandemic, Melissa Rosado

Theses and Dissertations

The forced expeditious transition online induced by the COVID-19 pandemic caused a massive disruption to education. COVID-19 exacerbated the inequities that existed in education for years. As technology advances and integrates with education, it can both enhance inclusive instructional practices, as well as exacerbate issues of equity. Achievement gaps will persist unless colleges create equitable educational environments for all students, including “at-risk” populations such as those enrolled in the Extended Opportunity Program and Services (EOPS) program—a state funded equity program.

Through the lens of digital equity and inclusion theoretical frameworks, the purpose of this embedded mixed methods study was to …

Parental Attitudes Regarding The Primary Dentition, Dental Decay, And Trauma, Kathryn Dundervill

Theses and Dissertations

Purpose: The purpose of this study is to identify the ability of parents in the VCU Pediatric Dental Clinic to recognize signs of decay and trauma and to determine their knowledge regarding the primary dentition.

Methods: This is a cross-sectional study that determined parental attitude and knowledge toward the function of primary teeth, childhood dental decay and dental trauma. Study data was collected through REDCap, a secure web-based survey application. Parents who brought their child to the VCU Pediatric Dental Clinic for a new patient examination or a recall examination were included in the study. The survey was only formatted …

Mathematical Models Of Infection Prevention Programs In Hospital Settings, Kelly A. Reagan

Theses and Dissertations

Hospitals play a vital role in providing for the healthcare needs of a community. Patients can develop hospital-acquired infections (HAIs) during their hospitalization due to exposure to foreign bacteria, viruses, and fungi. Infection prevention programs target and reduce HAIs, but implementing the infection prevention programs often comes with a cost. The goal of my research is to use mathematical models to quantify the impact of infection prevention programs on cases of HAIs and total healthcare costs. First, I use a Markov chain model to quantify how one infection prevention program reduces general HAIs in the hospital. Then, I calculate the …

Family Relationships And Academic Performance Via Belongingness Among Cuban Medical Students: Examining Family Legacy And Sex As Moderators, Maria J. Cisneros-Elias

Theses and Dissertations

Medical diplomacy is a foundational part of Cuban domestic and foreign policy (Feinsilver, 2010). Cuba has an abundance of doctors, encouraged by the country’s free medical education program (Hand et al., 2020), and has made a significant impact with its well-established healthcare system, provision of healthcare for all of its citizens, and healthcare support internationally. The current study aims to focus on processes underlying Cuban medical students’ academic performance, as they are a critical component of this successful system, and a population that has received limited empirical attention. Thus, the current study used path analyses to examine the relations between …

Academic Health Science Centers And Health Disparities: A Qualitative Review Of The Intervening Role Of The Electronic Health Record And Social Determinants Of Health, Wies Rafi

Theses and Dissertations

Literature on the magnitude of negative health outcomes from health disparities is voluminous. Defined as the health effects of racism, environmental injustice, forms of discrimination, biases in science, and sociological or socioeconomic predictors across populations, health disparities are part of an ongoing and complicated national problem that health equity programs are specifically designed to address. Academic Health Science Centers (AHC) institutions are a complex and unique educational-healthcare ecosystem that often serves as a safety net for patients in vulnerable and lower-income communities. These institutions are often viewed as one of the most uniquely positioned entities in the U.S. with an …

An Analysis Of The Special Focus Facility Program And Nursing Home Quality, Annie S. Rhodes

Theses and Dissertations

The Special Focus Facility Program is an intensive program meant to rapidly support 88 of the lowest quality nursing homes in the United States, as determined by the Five-Star Quality Ranking system. There are also 435 facility candidates which are similarly low performing but not enrolled in the program. Academic literature has largely ignored this program, and the existing grey literature is more than a decade old and does not include COVID-19 data. Applying a political economy of aging framework and using a case matching methodology, Special Focus Facilities (SFF), Special Focus Facility Candidate (SFFc) nursing homes, and 5-star nursing …

Modulation Of Effector Gene Expression In The Blood-Feeding Arthropods Cimex Lectularius Linnaeus, (Hemiptera: Cimicidae) And Aedes Albopictus Skuse, (Diptera: Culicidae), Travis C. Van Warmerdam

Theses and Dissertations

Hematophagy, or blood-feeding behavior, has independently evolved multiple times in the class Insecta and results in the transmission of vector-borne parasites, bacteria and viruses causing 17% of all infectious diseases and over 700,000 human deaths annually. One of the major challenges in studying the discrete mechanisms involved in host-response to hematophagy or the host seeking behavior of hematophagous insects, is the vast amount of signaling processes and genes that are inextricably tied to these interactions. These experiments used the clustered regularly interspersed short palindromic repeat (CRISPR)/CRISPR-associated (Cas) and dsRNA knock-down technologies to examine mechanisms of immunological reactivity and host-seeking behavior. …

Urban-Rural Differences In Lifespan Variation In The United States, Benjamin H. Walker

Theses and Dissertations

Since the mid-1980s, mortality rate improvements in urban areas have outpaced those in rural areas, leading to substantial urban-rural disparities in mortality. Research on urban-rural mortality disparities has focused exclusively on differences in mortality between urban and rural areas and has not examined differences in the amount of inequality in the length of life within these areas. Lifespan variation is an important dimension of health inequality that complements traditional metrics of mortality (i.e., mortality rates and life expectancy) by indicating the amount of inequality of lifespans within a population. This dissertation provides several contributions to our understanding of urban-rural differences …

Implementation Of Teach-Back For Discharge Teaching In A Critical Access Hospital: A Quality Improvement Project, Katie Beth Notch

Theses and Dissertations

A lack of comprehension of discharge instructions may cause high readmission and emergency room revisit rates for organizations. At the project site, there was no current evidence-based practice to ensure patient comprehension of discharge instructions. The purpose of this quantitative, quasi-experimental quality improvement project was to determine if the implementation of the Institute for Healthcare Improvement (IHI) Always Use Teach-Back Toolkit would impact emergency room revisit rates among adult medical-surgical patients in a critical access hospital in rural Minnesota over four weeks. Afaf Meleis’ transitions theory and the Iowa model for evidence-based practice were the scientific underpinnings of the project. …

A Statistical Comparison Of Covid-19 In The United States Across Political Affiliations And Census Regions, Margarito Torres

Theses and Dissertations

In mid-January 2020, the United States reported their first cases of the coronavirus disease (COVID-19) from a passenger returning from Wuhan, China. Initially, the situation wasn’t very alarming as in China and European countries, but the situation began to worsen in March 2020 when the number of cases began to multiply. Then, in a matter of a few months, the United States became the number one country in terms of total cases and total deaths from COVID-19. We have been closely observing the United States and the world since July 2020. Our study aims to compare the political affiliations and …

Data Analytics For Google Trend Search Result Of Illness Symptoms, Md Jakir Hossain

Theses and Dissertations

The outbreak of COVID-19 has escalated from March 2020. Since then, people from all over the world are curiously searching different types of illness symptoms including corona virus. Before the outbreak, people were also searching different types of symptoms at different times. Both diseases share same symptoms, but in the flu season. If certain types of symptoms are visible at summer season, then these symptoms are for corona virus. The main purpose of our study is to find out this discriminative information from these search result. We will discuss some mathematical concepts and then develop an algorithm based on those …

Factors Influencing Intent To Take A Covid-19 Test In The United States, Sheila Rutto

Theses and Dissertations

In 2020, COVID-19 became the first pandemic in the world’s history that brought the entire world to an abrupt and unexpected halt. Since the first reported case of the disease to date, the novel coronavirus has been able to wreak havoc in literary every corner of the globe and left an ever-growing number of unprecedented fatalities. The normal way of life has been disrupted, and the level of uncertainty about the end of this pandemic continues to manifest to many. Due to the urgency to bring this pandemic under control, medical officers have been able to recommend actions that people …

A Comparative Study On Feature Extraction And Classification/Clustering Of Fake News And Conspiracy Theories From Twitter Data, Deb Shana

Theses and Dissertations

Fake news and conspiracy theories have become largely abundant in the expanding world of social media. They predominantly affect the beliefs and thoughts of the public, resulting in chaos. They have always existed throughout the last few decades. They have been linked to prejudice, revolutions and genocide across history. They have also been known to have propelled people to reject mainstream medicines to an extent where some diseases are recurring in some parts of the world. They impose a serious impact since they are capable of spreading very fast Thus, it is very important to find suitable ways to detect …

Study Of The Effect Of B-Cell-Intrinsic Mhcii Antigen Presentation On Germinal Center B Cell Evolution Using The Brainbow Mouse Model, Nia Hall

Theses and Dissertations

Major histocompatibility complex class II (MHCII) are molecules that are essential for B cell activation, as well as clonal competition among activated B cell clones during germinal center (GC) reactions. However, it is unknown to what extent MHCII drives the selection of some clones over others. This is of particular importance since MHCII is highly polymorphic with the ability to indirectly alter gutmicrobiome composition by altering the immunoglobulin A (IgA) repertoire. To study this, we utilized a novel triple-transgenic mouse model to assess how B-cellintrinsic MHCII influences GC responses and GC clonal diversity, with an eyeforward to understanding the relevance …

The Immune Modulatory Role Of Endocannabinoid Anandamide To Suppress Inflammation Through Regulation Of Microrna And Microbiome, Muthanna Ali Sultan

Theses and Dissertations

Cannabinoids are group of compounds that exerts their anti-inflammatory action through activation of CB1 and CB2 receptors and other minor receptors. In the current study, we investigated the effect of Anandamide (AEA) on Acute Respiratory Distress Syndrome (ARDS) induced by exposure to Staphylococcus Enterotoxin B (SEB) intranasally. We found that C57BL/6 mice treated with AEA showed improvement in the clinical functions of the lungs, accompanied by a decrease in the infiltration of inflammatory cells in the lung tissue, and a decrease in the secretion of pro-inflammatory cytokines. Furthermore, AEA regulated the miRNA profile of the mononuclear cells in the lungs …

Food Choice Decision-Making Among School-Going Adolescents Amidst The Nutrition Transition In Urban Accra, Ghana, Krystal Krsna Rampalli

Theses and Dissertations

Like many countries of the world, Ghana is experiencing a nutrition transition and rising non-communicable diseases. Adolescents are susceptible to diet-related health risks as they experience significant physical and psychological changes, which are happening in tandem with food environment changes, including widespread proliferation of large portion and package sizes of energy-dense, nutrient-poor foods. Both local and multinational food and beverage companies have encouraged consumption of their products through various marketing tactics targeted directly to adolescents. Some of these tactics include the use of characters or celebrity endorsement, promotional discounts, and appeal to sociocultural values, including messages about body size preferences. …

Fate And Transport Of The Microbiome In Built Environments, Dahae Seong

Theses and Dissertations

Buildings are complex ecosystems evolving continuously. Indoor components such as occupants, ventilation system, and building structures influence the microbiome. To shed light on how the microbial population in a building will respond to such a fluid system, the fundamental interactions between microbes and the indoor ecosystem must be understood. Buildings are being designed as air-tight structures to save energy; however, this could lead to degradation of indoor air quality through indoor sources of contaminants and/or containment of pollutants in the room or introduce outdoor pollutants indoors depending on the ventilation conditions. In addition, transmission of infectious microbes in indoors threaten …

Utilization Of Remote Sensing To Analyze Aedes Mosquito Egg Abundance With Combined Weather Variables In Two Southeastern Coastal Cities, Danielle M. Johnson

Theses and Dissertations

Several vector-borne diseases have recently resurfaced, and many have suddenly spread to new areas in the United States (U.S.) due to climate change and tropicalization. Two vectors of public health importance are Aedes aegyptiand Aedes albopictus. The Southeastern region of the U.S. appears to be a preferential ecological niche for both Aedes species. Aedes aegyptiand Aedes albopictus are highly susceptible to environmental conditions, such as humidity, precipitation, temperature. Remote sensing technology has proven to be useful for estimating vector populations. Miami-Dade County is an established hotspot for disease transmission. Charleston County is newly vulnerable due to similar …

The Effect Of Low Dose Penicillin On Tumor Development In ApcMin/+ Mice, Kinsey Ann Sierra Meggett

Theses and Dissertations

Antibiotics have been our most effective weapon against bacterial infections since their discovery in the early 1900s. Their use has been critical in reducing mortality rate from infectious diseases. However, in the last few decades, the overuse of antibiotics, beginning at an early age and into adulthood, has become a growing concern globally. Penicillin is one of many extensively used antibiotics in early childhood that has been used to treat childhood infections. Recent studies showed that exposure to low dose penicillin can have adverse effects leading to chronic illness such as diabetes, allergies, inflammation, and susceptibility to obesity, with the …

Medication Assisted Treatment: Examining Enrollment And Demographic Factors Of Treatment, Antwana Letoya Drayton

Theses and Dissertations

Opioid Use Disorder (OUD) has been a long-standing epidemic concern related to the continuous growth of variations of the substance and its’ potency. Due to the versatility of the substance to be both an illicit drug and also a prescription medication, the interest in controlling and monitoring its’ use creates a greater public health concern. Treatment overtime has been revitalized to directly address the social, biological, and physiological difficulties of patients with OUDs. Medication Assisted Treatment (MAT) has been explored and has become one of the most widely used plans for treatment of Substance Use Disorders (SUDs) related to pain …

The Exploration Of Novel Synthetic Methods Of Biologically Relevant Nitrogen And Halogen Containing Molecules, Rebekah Erin Strong

Theses and Dissertations

Visible light has become a highly useful, and regarded, tool to the organic chemist. The additions and transformations of an abundance of molecules can be achieved with the use of visible light and photocatalysts, when appropriate. More specifically compounds that contain nitrogen, halogens, or both are highly useful structures in nearly every chemical industry. They are elements found in naturally occurring molecules and have the capacity to mimic biologically active and relevant structures. This makes them useful targets for pharmaceutical compounds. Bromine and chlorine can act as leaving groups; this property makes molecules with these elements reactive and thus able …

Designing Cell-Free Protein Synthesis Systems For Improved Biocatalysis And On-Demand, Cost-Effective Biosensors, Mehran Soltani Najafabadi

Theses and Dissertations

The open nature of Cell-Free Protein Synthesis (CFPS) systems has enabled flexible design, easy manipulation, and novel applications of protein engineering in therapeutic production, biocatalysis, and biosensors. This dissertation reports on three advances in the application of CFPS systems for 1) improving biocatalysis performance in industrial applications by site-specific covalent enzyme immobilization, 2) expressing and optimizing a difficult to express a mammalian protein in bacterial-based CFPS systems and its application for cost-effective, on-demand biosensors compatible with human body fluids, and 3) streamlining the procedure of an E. coli extract with built-in compatibility with human body fluid biosensors. Site-specific covalent immobilization …