Digital Commons Network^™

The Effects Of Ketones On Brain Metabolism And Cognition, Erin Reiko Saito

Theses and Dissertations

The brain is one of the most energetically demanding organs within the human body and is cognitively susceptible to energetic deficits such that the rise in obesity, insulin resistance, and Alzheimer’s disease in recent decades pose a substantial threat to cognitive longevity. The therapeutic efficacy of ketones are well-established in epilepsy and are currently being applied to other disease states. Alzheimer’s disease is characterized by impairments in brain glucose uptake and metabolism in regions relevant to learning, memory, and cognition that progress with the disease. While brain glucose uptake is impaired, ketone uptake is unaltered, potentially enabling ketones to fuel …

By All Memes, I Want To Learn Arabic: Teachers’ And Students’ Perceptions Of Using Memes In Afl Classrooms, Amani Batran

Theses and Dissertations

Abstract

As educational technology advances, teachers nowadays tend to incorporate technology into their classroom practices to enhance students’ learning and achieve better outcomes. Memes, which are pictures with texts and sometimes movements circulated mostly among social media users, are a powerful tool to reach learning goals in a fun and engaging way. Researchers explored memes’ effectiveness in various disciplines, yet the field of teaching Arabic as a foreign language (AFL) lacks investigation in this area. Therefore, this study aims to examine teachers’ and students’ perspectives on the use of memes in the classroom. This research also explores the disparities in …

A Comparison Of The Vascular Response To Acute Sauna Heating In Young And Middle-Aged Adults, Olivia Kathryn Leach

Theses and Dissertations

BACKGROUND: Age-related declines in endothelial function have been well documented with larger declines observed in middle-aged. Passive heat exposure has been shown to be a promising method to improve vascular endothelial health, with sauna specifically being linked to reduced risk of cardiovascular disease. Increases in blood flow and shear rates associated with heat exposure are often considered to have a major influence on the observed improved endothelial function following heat exposure. The magnitude of these changes in response to sauna have not yet been defined. Therefore, the purpose of this study is to quantify and compare the vascular response to …

Volume Change Measurements Of Cancer Cells In A Microfluidics Platform, Yukuan Yu

Theses and Dissertations

Understanding the function of aquaporins in transcellular transport is vital for homeostasis, pathophysiology, and translational potential in AQP-based diagnostics and therapeutics. Microfluidics device has the potential to evaluate the function of aquaporins with precise control and manipulate micro-scale fluid due to good optical quality and short processing distance. This work has two parts: cell culture in a microfluidic chip to characterize aquaporins’ function and visualization of single cell volume change under osmotic pressure shock in a homemade imaging dish. For the first part, we established a microfluidics platform for future modification and measurement of the dynamic volume change of biological …

Effects Of Date Seed (Phoenix Dactylifera) Extract In An Animal Model Of Diabetes Mellitus, Sara Refaat

Theses and Dissertations

Diabetes mellitus is a chronic illness and a global epidemic. The commercial diabetic medicines available are expensive and have several adverse effects. Alternative natural products are still being sought for. Date seeds ( Phoenix dactylifera) are either thrown away as garbage or utilized as animal feed. The use of seed extract as an alternative therapy for diabetes offers a cost-effective and all-natural method for managing the disease. Methods Sprague dawley rats were randomly distributed into groups with a sample size of 4 rats per group. Date seed powder was boiled producing a 10% date seed extract which was administered in …

Natural Fractals And Facial Emotion Recognition, Nathaniel Joseph Pagel

Theses and Dissertations

The psychological benefits that come from visually processing natural imagery have been widely studied. The components of natural imagery that provoke positive responses are not fully understood. This study uses a mixed design approach to identify if the acute exposure to statistical fractal imagery, of both a high-range or mid-range complexity, affects cognitive workload, physiological arousal, and gaze patterns in the recognition and identification of emotions through facial expressions. Participants were undergraduate students fulfilling a partial course requirement (N = 87). Eye-tracking metrics were used to compare exposure to fractal complexities. Participants were randomly assigned to either be exposed to …

Comparison Of Various Intermittent Fasting Dietary Interventions To The Ketogenic Dietary Interventions And Its Effects In Mitigating Hunger In Obese Adults, Aston Earl Francis Jr

Theses and Dissertations

There have been many dietary strategies that can be used for fat loss and the most recent popular dietary interventions have been intermittent fasting and the ketogenic diet. Intermittent fasting (IF) dietary interventions and the ketogenic diet (KD) are popular diets that claim to promote weight loss which has also been speculated to have enhanced benefits than caloric restriction. Although these dietary strategies are growing in popularity, it is unclear if the intervention can manage hunger, which may increase adherence. The purpose of this paper is to systematically review the literature on the impact of IF protocols compared to KD …

Methemoglobin Formation Via Nitric Oxide And Comparison Of Methemoglobin, Deoxyhemoglobin, And Ferrous Nitrosyl Hemoglobin As Potential Mri Contrast Agents, Roya Ayati

Theses and Dissertations

Gadolinium-based contrast agents (GBCAs) are in widespread use to enhance magnetic resonance angiography images for evaluating vascular pathology. However, there are safety concerns and limitations regarding the use of GBCAs. It has been shown that the magnetic resonance imaging (MRI) signal intensity (T1-weighted images) in some of the brain's tissues is higher for patients who had multiple exposures to GBCAs compared to patients who had never had exposure to GBCAs. This implies that GBCAs are not sufficiently removed from body such that GBCAs may potentially have long-term effects on the human body. These potential safety concerns have led to an …

Success And Confidence In, Or Inclusive Of, Undergraduate Chemistry Students Surrounding A Collaborative Learning Intervention, Encouragement Of Metacognition, And A Multifaceted Scholarship Support Program, Maggie Erin Leake

Theses and Dissertations

Student success in chemistry has been linked to a wide range of factors. Some of these factors are familiar, easily quantified measures; colleges typically rely on factors like high school GPA and measures of aptitude to make admission decisions or set course prerequisites. Success in chemistry courses can be linked to these measures, and math aptitude scores in particular are often used as prerequisites for introductory chemistry courses. However, success in chemistry can also be affected by factors like motivation, peer interactions, sense of belonging, and metacognitive skill. Additionally, outcomes in chemistry and other STEM courses like math and physics …

Improvement Of Ex Vivo Testing Methods For Spine Biomechanical Characterization, Aubrie Lisa Taylor

Theses and Dissertations

This dissertation contributed three main areas to flexibility testing and biomechanical characterization of human spinal segments. The first was a literature review of existing testing methodology, the second examined common spine fixation procedures, and the third developed an improved fixation method. Spine biomechanical characterization is the primary technique for assessing the healthy, diseased, and surgically treated response of spinal tissues. However, despite decades of use, no standard testing protocol or reporting methodology for flexibility testing conditions has been established. As part of the present work, a comprehensive, systematic literature review was performed, and the methods sections of 242 relevant journal …

Evaluation Of Cobb Mv X Cobb 500 Broiler Digestible Lysine Requirement And Response To Various Nutrient Regimens During The Finisher Phase, Dalton Gerard Dennehy

Theses and Dissertations

Previous research evaluated the dLys requirement and the impact of varying dLys and AME on Cobb MV x Cobb 500 broilers during the starter and grower phases, leaving the finisher phase to be determined. Experiment 1 estimated the finisher dLys requirement to range from 0.748-1.32% for males and 0.752-1.292% for females. Male broilers were more responsive to increasing dLys than females, though in general, increasing dLys improved performance and processing. Experiment 2 determined the effects of varying finisher dLys and AME on broiler performance and processing. Increasing dLys improved BWG, FCR, ALI, and breast yield while decreasing FI and fat …

Mapping For Meaning In A Multimodal Web: Advancing Learning Using Web-Based Instruction That Stimulates Wayfinding Behavior, Rhonda Lee Thomas

Theses and Dissertations

When learners cannot find their way through web-based instructional information, they become lost, as a result, learning stops. The operations of human wayfinding behavior (or simply, wayfinding) are the cognitive processes activated when humans must make decisions on how they will move through, primarily, novel situations. This paper examines the utility of incorporating the operations of wayfinding into instructional design models to reduce the likelihood of learners becoming lost in web-based instructional information.

The Applications Of The Internet Of Things In The Medical Field, Cody Repass

Theses and Dissertations

The Internet of Things (IoT) paradigm promises to make “things” include a more generic set of entities such as smart devices, sensors, human beings, and any other IoT objects to be accessible at anytime and anywhere. IoT varies widely in its applications, and one of its most beneficial uses is in the medical field. However, the large attack surface and vulnerabilities of IoT systems needs to be secured and protected. Security is a requirement for IoT systems in the medical field where the Health Insurance Portability and Accountability Act (HIPAA) applies.

This work investigates various applications of IoT in healthcare …

Robustness Of Muscle Synergies In Upper Limb Rotational Motion, Ashar Turky Abd

Theses and Dissertations

Muscle synergies (MS) are defined as the specific co-activation of muscle groups to accomplish functional movements. The hypothesis of muscle synergies helps simplify the motor coordination problem by decreasing the numbers of central nervous system signals. The purpose of this research was to investigate the number and the structure of muscle synergies for the upper limb 1) across participants for one power level 2) when the power levels increased and at a constant speed [50 r.p.m] and 3) including the synergies for pull and push for each participant during arm cycling on a stationary hand-cycle ergometer. We asked eight healthy …

Molecular Architecture Of Cardiometabolic Responses To Regular Exercise, Jacob L. Barber

Theses and Dissertations

Background: To investigate the molecular mechanisms, determinants, and predictors of cardiometabolic responses to regular exercise, this dissertation had two primary aims: 1) Examine the molecular architecture of cardiometabolic responses to exercise training (unsupervised approach) and 2) Identify molecular signatures of responsiveness to regular exercise within clinically relevant lipoprotein traits (supervised approach).

Methodology: Multi-omics data (17,945 muscle transcripts, 4,979 proteins, 411 metabolites) from participants (n=647) who completed 20 weeks of endurance training as part of the HERITAGE Family Study was collected before and after training. We employed an unsupervised machine learning approach (Aim 1) to identify patterns of molecular responses to …

Human Otop1 Proton Channel Can Function As A Monomer, Gabriella Abigail Ulloa

Theses and Dissertations

Otopetrins (OTOP) represent a new family of eukaryotic proton channels that do not share structural similarities with other renowned ion channels. Otop1 was initially known for its involvement in vestibular function and its significance for otoconia formation before it was established as a bona fide sour taste receptor. The cryo-EM structures for several Otop channels have been determined and demonstrated Otop1 as a homodimer comprised of two subunits. Each subunit constitutes twelve transmembrane helices that can be further partitioned into two structurally similar six transmembrane domains designated as the N and C domains. Molecular dynamic simulations have also previously shown …

Understanding Chinese International Students' Engagement And Belongingness In U.S. Universities, Yingying Jiang

Theses and Dissertations

Little research has been conducted on Chinese international students’ engagement and belongingness in U.S. universities. This study used a non-experimental quantitative survey research design and adapted survey items from past literature to target Chinese international students’ experiences. Prior to the survey administration, pretesting including expert reviews and a think-aloud were carried out to validate the survey. A total of 76 Chinese students from various universities participated in the survey data collection. The quantitative analytical processes included Confirmatory Factor Analysis (CFA) to ensure construct validity; measurement invariance testing between groups and Structural Equation Modeling (SEM) included four major factors/predictors (English proficiency, …

Arion 33: A Prospective Mission To Near-Earth Asteroids, Alita Regi

Theses and Dissertations

Near-Earth asteroids will become primary targets for space industrialization in the future as humanity becomes a multi-planetary species. But before such missions, it is necessary to survey these asteroids for their intrinsic scientific value. Exploring asteroids via orbiter missions can help us understand the asteroid's surface composition while providing us a cost and power model required for future missions. Therefore, it is beneficial to study asteroids to pave the way for future mining operations or more comprehensive scientific explorations. Hence, in this thesis, the target asteroids 2011 UW158 and 65803 Didymos are selected as case studies for the hypothetical mission …

The Beneficial Effects Of The Gut-Derived Metabolite Trimethylamine N-Oxide On Functional Β-Cell Mass, Emily Suzanne Krueger

Theses and Dissertations

Elevated serum levels of trimethylamine N-oxide (TMAO) were first associated with increased risk of cardiovascular disease (CVD) 10 years ago. Research has since defined that serum TMAO accumulation is controlled by the diet-microbiome-liver-kidney axis. Choline related nutrients are consumed in excess during over-nutrition from a Western diet. The resultant elevated serum TMAO is investigated across various chronic metabolic diseases and many tissue types. While TMAO is most clearly linked to CVD mechanisms in vascular tissue, its molecular effects on metabolic tissues are unclear. Here we report the current standing of TMAO research in metabolic disease context across relevant metabolic tissues …

Puma And The Innate Immune Response During Pneumococcal Infection In The Lung, Daniel Edward Kennedy Ii

Theses and Dissertations

Background: The p53-up-regulated modulator of apoptosis (PUMA) protein is a pro-apoptotic, BH3-only member of the BCL2 family of effector proteins responsible for promoting organized cell death. PUMA is required for resolution of pneumococcal pneumonia in mice, as mice deficient of PUMA exhibit greater numbers of S. pneumoniae CFU within tissues and higher mortality rates than observed in Puma+/+ mice. Methods: Puma+/+ and Puma-/- mice were intranasally challenged with TIGR4 pneumococcus and sacrificed 24 h post-infection. Differences in cytokine levels from blood and whole lung tissue were detected by MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel. Lung transcriptomes from Puma+/+ and …

Transit Mobility And Premature Birth In New York City: A Population-Based Study, Ross B. Maddux

Theses and Dissertations

Transit mobility has been recognized as an essential element to economic attainment, environmental sustainability, and as a vital part of public health⁠. Social and economic disparities paired with elusive and inefficient public transit present real barriers for the ability of efficient planning and use of time⁠. Lengthier commutes and generally less efficient travel contribute to stress during a pregnancy and limit the ability to access work and prenatal care. In survey studies of women utilizing clinics as their prenatal care provider, nearly 25% listed transportation as a barrier to accessing care.

Prior research has not linked directly premature birth and …

Virtual Screening For Potential New Chemotherapeutic Agents For The Gpr119 Receptor, A Target For Type Ii Diabetes, Jennifer Lizeth Bravo

Theses and Dissertations

Three frames from a molecular dynamics simulation run of a GPR119 receptor homology model were used for this study. The homology model was validated by virtually screening 76 known GPR119 receptor. 85% of these agonists bound to the receptor. Following the validation, 21,000 molecules were selected for the virtual screening study. 862 ligands came from the GPCR Selleckchem/Prestwick library, 42 compounds from the Prestwick Phytochemical library, 20,000 compounds from the ZINC library, plus four molecules from the literature. All ligands were built, geometry-optimized, and docked in the GPR119 models using a protocol combining High Throughput Virtual Screening, Standard Precision, and …

Effectiveness Of Equine Assisted Psychotherapy & Learning For Substance Use Disorder Treatment In College-Age Young Adults, Katherine Abigail Cagle-Holtcamp

Theses and Dissertations

With overdose deaths and residential addiction treatment admittance increasing, more treatment options are necessary to meet the demand of services. Equine assisted psychotherapy and learning (EAPL) is a relatively new therapeutic tool in addiction treatment for individuals battling substance use disorder (SUD), and thus, lacks a solid evidence base to be implemented consistently and effectively. The purpose of this dissertation was to determine the effectiveness of EAPL in creating an emotionally safe environment for learning for college-age young adults with a particular focus on those struggling with SUD. This was accomplished in a series of projects focused on: 1) implementing …

Disabled Epistemologies: Failures Of Knowledge And Care In Shakespeares's Merchant Of Venice And Othello, Amie Elisabeth Wambach

Theses and Dissertations

The presence of disabled characters like blind Gobbo in The Merchant of Venice and epileptic Othello are handy physical metaphors for the failures of epistemology that occur in both plays. Disability is often construed as a sort of saboteur of knowledge—disability of all kinds inhibiting the ability to perceive the world as an abled person would. But disability also produces a new, necessary sort of knowledge in order to survive and thrive in an unaccommodating world. A disabled epistemology suggests that knowing is contingent on individual, specific experience of the world. Tied to this issue of disabled epistemology is the …

Costs And Benefits Of Physical Therapy Program Implementation For Air Force Fighter Pilots, Christian G. Erneston

Theses and Dissertations

Air Force fighter pilots face risks associated with neck and spine injuries sustained while operating fighter aircraft. Studies from the flying and medical communities indicate that muscle-strengthening prehabilitative care may decrease the risk of flying related injuries in high performance aircraft pilots. For this reason, the U.S. Air Force provided $24.9M to implement the Optimizing the Human Weapon System (OHWS) program. The program provides physical therapy and strength training to fighter pilots in participating units at twenty-one Air Force bases with the intent of reducing injury rates and time out of the cockpit. From a healthcare perspective there is interest …

Establishing A Mechanistic Link Between Disturbed Flow And Aneurysm Formation In A 3d Cerebral Bifurcation Model, Nesrine Bouhrira

Theses and Dissertations

The effect of disturbed flow profiles on the endothelium have been studied extensively in systemic vasculature, but less is known about the response of the blood-brain barrier (BBB) to these flow regimes. Here we investigate the effect of steady and pulsatile disturbed flow on the integrity of the BBB using a three-dimensional, perfusable bifurcation model consisting of a co-culture of endothelial cells with mural and glial cells. Experimental flow patterns predicted by computational fluid dynamics mimic in vivo flow regimes, specifically the presence of a recirculation zone immediately downstream of the bifurcation reveal periodic changes in the instantaneous shear stress …

Experimental Pharmacotherapies In Models Of Alcohol Addiction, Mousumi Akter Sumi

Theses and Dissertations

The USA faces $220 billion economic loss and eighty thousand deaths per year due to alcohol abuse while affecting more than 15 million people, making it the third largest life-style related cause of death. The U.S. FDA has approved four medications namely, disulfiram, acamprosate, oral naltrexone, and injectable long-acting naltrexone. These existing drugs are trashed with side effects, have a low success rate, indicating a demand for new potential drugs. We studied the effects of the CB1 negative allosteric modulator, PSNCBAM-1, and the dopamine D4 receptor antagonist, L-745,870 in mouse models of alcohol addiction. PSNCBAM-1 did not significantly reduce CPP …

Estimating Affective States In Virtual Reality Environments Using The Electroencephalogram, Meghan R. Kumar

Theses and Dissertations

Recent interest in high-performance virtual reality (VR) headsets has motivated research efforts to increase the user's sense of immersion via feedback of physiological measures. This work presents the use of electroencephalographic (EEG) measurements during observation of immersive VR videos to estimate the user's affective state. The EEG of 30 participants were recorded as each passively viewed a series of one minute immersive VR video clips and subjectively rated their level of valence, arousal, dominance, and liking. Correlates between EEG spectral bands and the subjective ratings were analyzed to identify statistically significant frequencies and electrode locations across participants. Model feasibility and …

Effects Of Hiv-1 Gp120 On Intracellular Levels Of Iron And Reactive Oxygen Species: Implications For Lysosomal Stress, Koffi L. Lakpa

Theses and Dissertations

Approximately 1.2 million people in the U.S.A are living with HIV-1. The development of antiretroviral therapeutics has increased the life expectancy of people living with HIV-1 (PLWH). Yet, there is a growing concern for aging HIV-positive individuals because about 50% of PLWH develop neurological impairments, termed HIV-associated neurocognitive disorders (HAND). There are no treatments for HAND and the mechanisms underlying its pathogenesis remain unclear. Several factors are implicated in HAND pathogenesis, such as HIV-1 coat protein gp120, endolysosome and mitochondrial dysfunction, and elevated levels of reactive oxygen species (ROS). Endolysosomes are acidic organelles that contain readily releasable stores of cations, …

The Role Of The Chaperone Cct In Assembling Cell Signaling Complexes, Nicole C. Tensmeyer

Theses and Dissertations

In order to function, proteins must be folded into their native shape. While this can sometimes occur spontaneously, the process can be hindered by thermodynamic barriers, trapped intermediates, and aggregation prone hydrophobic interactions. Molecular chaperones are proteins that help client proteins or substrates overcome these barriers so that they can be folded properly. One such chaperone is the chaperonin CCT, a large MDa protein made up of 16 paralogous subunits that form a double ring structure. CCT encapsulates its substrates in a central cavity, where they are sequestered and folded, using ATP binding and hydrolysis to drive conformational changes in …