Digital Commons Network^™

Decoding Prmt1: Studies On The Catalytic Mechanism, Regulation, Inhibition, And Crosstalk Of Prmt1-Dependent Methylation, Heather L. Rust

Theses and Dissertations

Arginine methylation is catalyzed by the protein arginine methyltransferase (PRMT) family of enzymes, which transfer a methyl group from S-adenosylmethionine (SAM) to the guanidinium group of an arginine residue. This reaction first produces monomethylated arginine (MMA) that can then be further methylated to produce either asymmetrically dimethylated arginine (ADMA) or symmetrically dimethylated arginine (SDMA). There are nine PRMT family members described to date, with PRMT1 as the predominant member, suggested to be responsible for ~85% of asymmetric dimethylation. In addition, PRMT1-dependent methylation likely plays a significant role in a plethora of diseases (e.g., cancer, heart disease, and ALS). These observations …

Iii-V Nitride Micro- And Nano-Scale Cantilevers For Multimodal Sensing Applications, Ehtesham Bin Quddus

Theses and Dissertations

Recent research trends in chemical and biological sensing have been geared toward developing molecular sensor devices that are fast, label free, miniaturized and portable. The performance of these devices can be dramatically improved by utilizing multimodal detection techniques, new materials and micro-/nanofabrication technologies. This is especially true for micro-/nanoscale cantilever sensors, which undergo changes in mechanical or electrical properties upon the specific binding of molecules. To develop the sensor devices with the above attributes, we utilized III-V nitride materials: InN nanowires for realizing nanoscale cantilevers and AlGaN/GaN heterostuctures with or without embedded HFETs, for developing microcantilevers. There are mainly two …

Silylation-Based Kinetic Resolution Of Α-Hydroxy Carbonyl Compounds And Synthesis Of Chiral Isothiourea Catalysts, Yan Zhang

Theses and Dissertations

The thesis describes the silylation-based kinetic resolution for α-hydroxy carbonyl compounds catalyzed by isothiourea catalyst as well as our effort to make novel chiral isothiourea catalyst.

In Chapter 1, general background will be introduced, including chirality, general methods to obtain enantiopure compounds and kinetic resolution. In Chapter 2, the mechanism of the chiral recognition was discussed and a novel isothiourea catalyst was design to investigate the mechanism. The synthesis of the new catalyst was also described in this chapter.

In Chapter 3, the silylation-based kinetic resolution for α-hydroxy carbonyl compounds will be discussed. Since silylation-based kinetic resolution have already been …

High Quality Silicon Carbide Epitaxial Growth By Novel Fluorosilane Gas Chemistry For Next Generation High Power Electronics, Tawhid Rana

Theses and Dissertations

High quality, thick (~100µm), low doped and low defect density SiC epitaxial films are essential for high voltage (blocking voltage >10kV), light, compact and reliable next generation power devices. One of the significant challenges in obtaining high quality thick SiC epitaxial films is to restrict/eliminate the Si gas-phase nucleation or aerosol formation during growth. The generated aerosol particles adversely influence growth by reducing the growth rate due to precursor losses, and also affect crystal quality, since the Si droplets are carried to the crystal growth surface. Moreover, liquid aerosol particles adhere to the various reactor parts (parasitic deposition), and contribute …

The Development Of The Femme Fatale On Stage As Seen In The Works Of Jesse Lynch Williams And Edna St. Vincent Millay, Hillary Elizabeth Macarthur

Theses and Dissertations

The Femme Fatale in the United States developed from certain characteristics represented by the New Woman movement of the 1920's. Jesse Lynch Williams and Edna St. Vincent Millay capture the identifying factors of what a Femme Fatale is and should be. This paper will explore the differences and similarities in the images of the Femme Fatale and New Woman, and discuss how those differences are related to origins of the image of the New Woman.

Congeries In The Backcountry, James Andrew Stewart

Theses and Dissertations

The early 18th century Public Monopoly employed a unique form of labor organization to facilitate an administered deerskin trade. This study examines ethnohistoric and archaeological data as a means to evaluate subaltern laborers' participation in a commercializing colonial economy. It utilizes a practice based political-economic framework to situate their activities within the deerskin commodity chain. Activities identified through ethnohistoric research are evaluated through material and spatial analysis of the Fort Congaree, 38LX30/319, site. This trading factory and garrison played a central part in the articulation of the government deerskin trade. Material correlates of hide production, procurement, and transportation activities contextualize …

Re-Examining The Past And Rethinking The Future At Mount Mulanje Forest Reserve, Malawi: New Directions For Local Engagement, Mary Christian Thompson

Theses and Dissertations

Since the 1980s, broad recognition has been given to the need for and the benefits of aligning the protection of biodiversity in threatened forest ecosystems with measures to address the needs and desires of people living near and depending on those ecosystems. With this research project I focus on one such ecosystem found at the Mulanje Mountain Forest Reserve (MMFR) in southern Malawi. Large amounts of money and time have been put forth by local, national, and international donors and conservation organizations to support the goals of biodiversity conservation and social development at MMFR. In order to explore how managers …

Computational Analysis And Prediction Of Genome-Wide Protein Targeting Signals And Localization, Jhih-Rong Lin

Theses and Dissertations

Computational prediction of protein subcellular localization can greatly help to elucidate its functions. Despite the existence of dozens of protein localization prediction algorithms, the prediction accuracy and coverage are still low. Several ensemble algorithms have been proposed to improve the prediction performance, which usually include as many as 10 or more individual localization algorithms. However, their performance is still limited by the running complexity and redundancy among individual prediction algorithms. In the first part of the dissertation, we propose a novel method for rational design of minimalist ensemble algorithms for practical genome-wide protein subcellular localization prediction. The algorithm is based …

Quantifying The Effects Of Antibiotics Upon Benthic Diatoms And Marine Benthic Algal Communities, Isaac M. Hagenbuch

Theses and Dissertations

Antibiotics are a cornerstone of modern medicine. Their ability to impair prokaryotic metabolism has saved the lives of many millions of humans in the decades since wide antibiotic usage began. Antibiotic compounds are also widely used in the husbandry of animals that are used for food but, unfortunately, much of the antibiotic mass that goes into humans and animals is excreted unchanged. Those excretions go, directly or indirectly, into aquatic ecosystems. The discovery of this fact has led to a growing global concern about the fate of antibiotics in the environment. This concern is driven by no fewer than three …

Promoting Early Childhood Development Through Community Health Worker Interventions: Implementation And Measurement Issues In Rural Pakistan, Zaeem Haq

Theses and Dissertations

Appropriate nutrition and psychosocial stimulation during the first three years of life can have long-lasting effects on a child's health and development. However, not all children in the world receive adequate nutrition and psychosocial stimulation during this period. There has been enormous interest in developing the evidence on the most effective ways of implementing and assessing early childhood development (ECD) promotion interventions in resource poor settings. Despite the interest, the integration of ECD interventions into pre-existing public health programs, and assessment of these interventions in extended family, rural household environment have not been addressed.

The dissertation research presented here was …

Improved Limits And Portability Over Currently Employed Cadmium Monitoring Systems Through Preconcentration For Detection By Way Of Micro-/Nanofluidic Mechanisms, Paul F. Wach

Theses and Dissertations

Due to risk of environmental and biological accumulation of Cadmium (Cd), improved methods of early detection and monitoring must be explored as a preventative measure. Listed as one of the top three toxic heavy metals by the Environmental Protection Agency (EPA), the effects on ecological and human systems have well documented side-effects of physical mutation, reproductive sterility, kidney failure, liver disease, bone loss, and death. Found in batteries, metal plating, pigments, plastics, and cigarettes, Cd is also used as a neutron absorber in the nuclear industry as well as having 3 known radioactive isotopes. Urine Cd levels, which have been …

The Meaning And Purpose Of Quotation And Stylistic Reference In Hans Werner Henze's Requiem: Nine Sacred Concertos For Piano Solo, Trumpet Concertante, And Large Chamber Orchestra (1990-92), Daniel C. Pappas

Theses and Dissertations

Quotation and stylistic reference are important communicative devices in the musical language of Hans Werner Henze that serve as a means of expression in his multilayered approach to composition. This study examines the central role that quotation and reference play in Hans Werner Henze's Requiem: Nine Sacred Concertos for piano solo, trumpet concertante, and large chamber orchestra (1990-92). While some studies have identified selected references, their primary focus has been on the work's pitch organization and formal plan. This study instead considers Henze's unique approach to quotation and reference. In addition to interviews with the composer, Henze's own writings are …

K+ Channels And Beta3 Adrenergic Receptors As New Pharmacological Targets For Overactive Bladder Treatment, Serge Amani Yao Afeli

Theses and Dissertations

Overactive bladder (OAB) is a pathophysiological condition which is characterized by the urinary bladder's failure to achieve proper storage of urine. OAB has a tremendous impact on the patient's quality of life by disturbing his sleep, work, and sexual activity. About 17% of Americans are affected with this disease and most patients experience symptoms of urgency, frequent urination, and abnormally elevated detrusor smooth muscle (DSM) contractility. Despite, numerous attempts to treat the disorder in the past with antimuscarinics and now with beta3 adrenergic receptors (beta3-AR agonists), OAB still remains a serious public health issue because the long term efficacy of …

The Definition Of Youth, Jessta Noel

Theses and Dissertations

The purpose of this project was to write my own theatrical production which would solely include me as the actor. I drew upon my experience of childhood growing up in Atlanta, GA as well as the stories passed on in my family.

Using Singular Value Decomposition In Classics: Seeking Correlations In Horace, Juvenal And Persius Against The Fragments Of Lucilius, Thomas Whidden

Theses and Dissertations

For the purpose of this dissertation, the hypothesis is posited that a programmatic correlation of the poems of Lucilius and the other Satirists reveals a detailed and dense level of intertextuality, especially in those poems which scholars already understand to allude to the genre's inventor. In addition to those poems which are discussed in secondary literature, we have discovered other poems which correlate highly with the corpus of Lucilius, but have been largely ignored. To demonstrate this fact I have devised a method using Singular Value Decomposition. That method is able to discern this subtle intertextuality in both the texts …

The Finer Things, Jasmine Bailey

Theses and Dissertations

It is through the grotesque that Flannery O'Conner's characters achieve grace, and often the only hope for self-actualization rests in death and malformation. This is perhaps her greatest irony. The protagonists of The Finer Things, however, never self-actualize: they simply act without understanding or questioning why. This is because they are rarely confronted with the grotesque; instead they actively seek it for themselves. The grotesque isn't revelatory, but rather, it satisfies romantic ideals and desires, or is pursued in effort to escape aggressive bureaucracies that typify Kafka's short stories. This characterizes the ironic aesthetic of The Finer Things. Working in …

Identifying Values And Benefits Of Congaree National Park, Maka Bitsadze

Theses and Dissertations

Protected areas are important tools for the conservation of biological diversity. They also support a wide range of ecosystem services and provide economic, cultural and social values and benefits. Many values and benefits of protected areas are poorly understood and greatly under-valued by decision-makers, the business sector, and the general public. Accordingly, identification and assessment of values and benefits of protected areas has been increasingly promoted during recent years.

This research investigates values and benefits of Congaree National Park and provides guidance for the further economic valuation of identified values and benefits. While the ecological significance of Congaree National Park …

An Examination Of Disgust, Its Measures, And Gender Differences In The Experience Of Disgust Sensitivity, Diane M. Kedzierski

Theses and Dissertations

The emotion of disgust is understudied. It has been implicated in various forms of psychopathology, but its overall influence remains unclear. New and improved methods and constructs are required if we are to better understand the relationship of disgust in attitude formation and psychological functioning. This study was an investigation of a measure referred to herein as the Disgust Propensity and Sensitivity Evolutionary Scale (DPSES). A total of 655 participants were recruited from a private university in the southeastern United States to complete a pencil -and-paper version of the measure in exchange for course credit. Exploratory factor analysis revealed an …

Effects Of Short-Term Dewatering On Grazer Abundance And Algal Biomass In A Wastewater Treatment Algal Turf Scrubber, Emily Husted

Theses and Dissertations

Finding a new, clean, and sustainable energy source to replace fossil fuels is one of society’s most daunting challenges. Algae biomass is a promising renewable resource for biofuel production due to its efficient conversion of solar energy into chemical energy. Algal turf scrubbers (ATS™) systems mimic natural streams, flowing wastewater over hard substrates designed to encourage attached algae growth. This study focuses on the use of replicated ATS™ systems, because they are low maintenance, are effective for removing nutrient pollutants, model natural stream ecosystems, and can produce large volumes of harvestable algal feedstock for biofuel production. A challenge that occurs …

Biomaterial-Induced Osteogenesis Of Mesenchymal Stem Cells By Surface Roughness And Functionalization, Pongkwan Sitasuwan

Theses and Dissertations

For tissue engineering and regenerative medicine, it is important to understand factors directing the stem cell fate, including self-renewal, proliferation, differentiation, and apoptosis. Bone marrow derived mesenchymal stem cells (BMSCs) have the potential to differentiate into osteoblasts, chondrocytes, adipocytes, and smooth muscle cells. Specifically, in the field of bone tissue engineering, BMSCs are commonly used for in vitro osteogenesis studies. However the mechanisms and signaling pathways that these cells recognize material surface and utilize to differentiate are still unclear. This dissertation focuses on investigating the effect of surface nanotopography and functionalization on the promotion of osteogenic differentiation of BMSCs. Surface …

The Role Of Trickster Humor In Social Evolution, William Gearty Murtha

Theses and Dissertations

Trickster humor is ubiquitous. Every society has some version of trickster and each society tells the stories of trickster over and over again to both enlighten and entertain. This thesis argues that trickster humor plays a fundamental role in helping society adapt by challenging social norms. Because trickster stories are humorous they are entertaining, because they critique social behaviors they are instructive. Tricksters break social rules, leaving society to remake them. This thesis examines the works of American Humorists Tom Robbins and Edward Abbey, particularly Still Life with Woodpecker and The Monkey Wrench Gang, arguing that these authors are contemporary …

Building Resiliency: Supporting Elementary School Students Through Parental Bereavement, Sandra Kerr Ray

Theses and Dissertations

This study employed qualitative methods to learn what understandings, practices, and interventions were used by educators from one elementary school to support bereaved children in the classroom and as members of a school community. Educators reported uncertainty about how children grieve, how to talk with children about a deceased parent, how to talk with the bereaved child’s peers about parental death, and how to balance the bereaved child’s emotional and academic needs. Educators were also uncertain about how to recognize and intervene when a parental death was remote in time and the child’s grief response was attenuated (e.g., anxiety, disengagement, …

A Critical Review Of Endometriosis Pathology, Brittany Lauren Cureton

Theses and Dissertations

Endometriosis is a complex, multifactorial, reproductive disorder present in approximately 10-15% of adult women between the ages of 25-35. This disorder occurs when endometrial glands and stroma grow ectopically on the surface of the ovaries, pelvic peritoneum, fallopian tubes, and the uterus. Endometriosis causes varying degrees of painful symptoms and infertility in infected individuals. Three main theories of endometrial accumulation attempt to explain the etiology of this elusive disease. There have been various staging of endometriosis symptoms that attempt to standardize classification as well as predict pain and infertility. Angiogenesis, necessary to the survival of endometrial tissue, along with immune …

The Reproductive Ecology Of Plethodontid Salamanders In The South Carolina Inner Coastal Plain, Heather Rena Oswald

Theses and Dissertations

Nest- site selection is a critical process in the life history of amphibians, directly influencing offspring survival and parental fitness. When and where an amphibian chooses to nest is strongly influenced by a range of environmental cues. We monitored plethodontid salamander breeding behavior in seepage wetlands in the South Carolina inner Coastal Plain. Seepage wetlands are ideal for monitoring semiaquatic salamander breeding, because they provide habitat for both larval and adult salamanders while having relatively predictable, constant water temperatures and presence year round. We observed the breeding phenology of three sympatric salamanders (Eurycea cirrigera, Desmognathus auriculatus, and Pseudotriton ruber) in …

Osteon-Mimetic Nanocomposite Materials For Bone Regeneration, Ozan Karaman

Theses and Dissertations

The limitations of large bone defect regeneration has brought the focus of Bone Tissue Engineering research on fabricating three-dimensional bone-mimetic grafts that could enhance osteogenesis and vascularization. Osteons composed of apatite-deposited aligned collagen nanofiber layers are the fundamental building blocks of cortical bone. In natural cortical bone, mineralization is mediated by extracellular matrix (ECM) proteins with amino acid sequences rich in glutamic acid. This dissertation is focused on the establishment of a novel biomimetic approach to engineer scaffolds for healing large bone defects.

In the first part, we investigated the effect of calcium phosphate (CaP) deposition on aligned surface-modified nanofibers …

Selling The Country’S Secrets: Willa Cather’S Eco(Self)Criticism In My Antonia And The Professor’S House, Elizabeth Nicole Morgan

Theses and Dissertations

This study considers Willa Cather's ecological consciousness as a writer of place, particularly in My Antonia and The Professor's House. In these two works, Cather's narrative distance provides her with the room to investigate the relationship between humans and their environments. Jim Burden, Godfrey St. Peter, and Tom Outland all exploit their environments to greater or lesser extents based on their way of seeing the world, which Cather draws attention to through her careful characterization and narrative distance. In each narrative, Cather forces readers to recognize the environmental consequences of egocentric vision, and the way such vision can be sustained …

Decision-Support Tool For Residential Pesticides In The South Carolina Coastal Zone, Lisa Claire Wickliffe

Theses and Dissertations

The Environmental Protection Agency (EPA) is charged with ensuring pesticides do not pose unreasonable adverse risks to the public and to the environment. This is a daunting task with over one billion pounds of pesticides used across the nation each year. The U.S. EPA estimates approximately 75% of all pesticide usage in the U.S. are agricultural while 25% is for home, garden, industrial, commercial, and government applications. One area of application of concern to public health and the environment regarding misuse of pesticides is in residential settings. In these instances, individuals may not have any knowledge of identifying whether they …

Outsmarting The Brain: Augmenting Motor Training With Non-Invasive Brain Stimulation In Order To Facilitate Plasticity-Dependent, Functional Improvement Within The Motor Cortex, Raymond Joseph Butts

Theses and Dissertations

Brain stimulation techniques capable of optimizing cortical plasticity may provide the key to improved therapeutic techniques and functional outcomes. The primary aim of this dissertation was to examine the potential of motor training (MT) augmented with intermittent theta burst stimulation (iTBS) and anodal transcranial direct current stimulation (a-tDCS). The secondary aim was to investigate whether the training would also be advantageous to older-adults. We hypothesized that right-handed, college-age students exposed to the treatment (n=17) would perform better short-term (directly following MT) and long-term (24 hours and 7 days following MT) on motor-skill retention tests than students receiving sham stimulation (n=14). …

The Effects Of A High Fat Diet On Mitochondrial Biogenesis And Inflammation In The Brain., Lauren Suzanne Vervaecke

Theses and Dissertations

INTRO: High fat diets (HFD) ingested over long periods of time have been shown to cause diet induced obesity leading to excessive adiposity and chronic low grade inflammation. Here we have shown how differing levels of saturated fats (SF), which are common storage fats, affect markers of inflammation and mitochondrial biogenesis. IL-1â and TNF-alpha are pro-inflammatory cytokines that have been shown to increase with a HFD, while molecular signalers, PGC-1alpha, cyt c, and SIRT1, related to mitochondrial biogenesis have been shown to decrease. Despite the risks of consuming a diet high in SF's, consumers continue to buy energy dense foods …

I Can Be Silent And Be Saying A Lot: Teachers' Racial Literacy In A Southern Elementary School, Kimberly J. Howard

Theses and Dissertations

In order to better understand how teachers make sense of race in schools today, this ethnographic study explores the following research question: How do teachers in this school make sense of race, and how does the spatiality of the school inform this process? The study was conducted over a 14-month period in a southern elementary school and is presented as a poetic, narrative, and thematic analysis of the connections between the geographic location of this particular school and the teachers' practices, pedagogies, and conversations about race both inside their classrooms and in other school spaces. This study demonstrates how teachers' …