Digital Commons Network^™

Reply Stop To Stop, Molly A. M. Garrett

Theses and Dissertations

Over the course of 8 years working in progressive political strategy, 2015–2023, I became increasingly critical of the systems, formulas and tools we were using to communicate with voters. Reply STOP to Stop is documentation of my time at graduate school, of wrestling with ideas of political design, language, communication and distribution. I analyzed my past work — all the texts, emails, social media posts, print ads, web banners, videos and other miscellaneous scripted content — and wondered if we would all be better off without it. Would elections be fairer, would there be less fake news, more community building? …

Watchpeopledie . Tv, Noah Lebien

Theses and Dissertations

The thesis piece is a performance called watchpeopledie.tv performed four times at the Anderson Gallery in the Hidden Galleries. There are two rooms in the gallery. The first room is where the audience enters. There is a line on the floor marking that the audience is not to move further into the first room or into the second room. The audio consists of verbatim comments sourced from videos of violence against trans people from the website watchpeopledie.tv, a website in which users post real videos of shootings, suicides, car accidents, etc. The main text, which is my thesis writing below, …

Developing Bioinformatics Expertise Through The Annotation Of The Gut Microbiome Of Odontotaenius Disjunctus, Keidra E. Carter

Theses and Dissertations

Next-generation sequencing (NGS) technologies have revolutionized biological research by generating unprecedented volumes of genomic data, necessitating advanced bioinformatics tools and specialized expertise to fully interpret complex microbial communities. This study outlines an integrated workflow to characterize the gut microbiome of the wood-feeding beetle Odontotaenius disjunctus. Prokka is employed for functional annotation to identify protein-coding genes and other genomic features, while Kaiju is used for taxonomic classification. DIAMOND is then applied to locate and validate the taxonomic assignments generated by Kaiju by aligning sequences within the contigs to a comprehensive protein database, proGenomes. Functional analysis revealed that the contigs encode proteins …

Radical Hope Amid Generational Oppression And Puerto Rican Well-Being, Natalia Gomez Giuliani

Theses and Dissertations

Oppressive systems (e.g., colonialism, racism, capitalism) create a disparate distribution of power that, through various mechanisms, negatively impacts the health and well-being of members of the oppressed group. Boricuas have experienced over 500 years of oppression, with the island currently maintaining a colonial relationship with the United States. As these adverse effects of oppression are identified, the need for protective factors (e.g., hope) has emerged. This study explored how islander Boricuas practice radical hope, understand their history of oppression and resistance, and how they perceived it to impact their overall well-being. Utilizing a phenomenological qualitative approach, I conducted 15 semi-structured …

Climate Change-Induced Shifts In Mediterranean Fungal Fruiting Phenology And Productivity, Rachel Demay

Theses and Dissertations

We used a 29-year dataset of mushroom fruiting from the Pinar Grande forest in northern Spain to investigate the effects of climate change on the phenology, productivity, and diversity of mycorrhizal and saprotrophic mushrooms. We investigated the long-term trends in fruiting season length, yield (by count, total weight, and average weight), and genus richness. Additionally, we identified associations between monthly temperature and monthly precipitation with these fungal responses across functional groups: mycorrhizal, saprotrophic, litter-decaying saprotrophic, and wood-decaying saprotrophic mushrooms, and the economically-important edible genera Boletus and Lactarius. We found that autumnal season length in the Pinar Grande has shortened over …

Narratives Of Illness Identity Development In Adolescents With Cancer, Rachel Holder

Theses and Dissertations

Introduction: Adolescence is a pivotal developmental stage characterized by the development of independence, autonomy, and identity. A diagnosis of cancer can be disruptive to this process (Grinyer, 2009; Hazen et al., 2008; Neylon et al., 2023). How one incorporates cancer into their identity, or illness identity, has been associated with clinical outcomes, including adherence, life satisfaction, psychosocial functioning, and health-related quality of life and could be a target for supportive interventions aimed at adolescents with cancer (Commissariat et al., 2020; Luyckx et al., 2008, 2018; Meyer & Lamash, 2020; Oris et al., 2016; Raymaekers et al., 2020). Prior literature has …

Rehabilitation In Stroke-Related Flexor Synergy, Shawn J. Dirocco

Theses and Dissertations

Upper extremity abnormal flexor synergy caused by stroke can impair individuals by coupling upper extremity muscles with deltoid activation. Previous studies have shown that reduction in deltoid loading can decrease flexor synergy. Robotic rehabilitation has the ability to allow for reduced deltoid loading while assisting or constraining joints along the arm to increase rehabilitation outcomes. The effects of higher degree of freedom low impedance robotics needs to be tested to learn how various parameters affect flexor synergy in comparison to end point robotics. During the first experiment, we tested 16 chronic stroke patients with flexor synergy problems in 3 tasks. …

Intuitive Mixed Reality Interfaces For Creative And Interactive Robotics: Roboart, Magnaforma, And Playmeow, Miles Popielamc

Theses and Dissertations

This thesis details the development and application of intuitive Mixed Reality (MR) interfaces designed to broaden access to robotic control for creative and interactive uses. The central contribution is the RoboART system, a novel MR interface built with the Microsoft HoloLens 2 to program an ABB GoFa collaborative robot for non-expert users, such as artists. The work describes RoboART's application in the Magnaforma project, where it enabled complex artistic expression.

Additionally, the thesis introduces the PlayMeow project, which explores adaptive pet interaction through a laser-guided turret. This system's control strategies were deeply informed by the intuitive paradigms developed for RoboART, …

Characterization Of Nanoplastic-Induced Exacerbation Of Asthmatic Pathophysiology In A Murine In Vivo Model, Keith Li

Theses and Dissertations

As global plastic production increases, so does the environmental and biological presence of nanoplastics (NPs), raising growing concerns about their potential impact on human health. These particles have been detected in various organs, including the lungs, yet their role in respiratory inflammation remains largely unexplored. Emerging evidence suggests that NP exposure may influence immune responses in the lungs, particularly in individuals with preexisting conditions such as asthma. However, the specific ways in which these particles interact with allergen driven inflammation are still unclear.

In this study, a murine model of allergic airway inflammation was used to investigate how surface modified …

Bugs In The Flux: Links Between Arthropod Diversity And Spatial Variation Of Soil Respiration In A Temperate Forest, Erin-Darby H. Mcclain

Theses and Dissertations

Soil respiration (Rs) is the largest terrestrial flux of carbon (C) to the atmosphere, with heterotrophic contributions to Rs varying spatially and temporally. While microbes are the primary and most thoroughly studied source of soil heterotrophic respiration (Rh), contributions from higher trophic organisms, including arthropods, are rarely quantified and poorly understood. Arthropod abundance and diversity correspond with variation in soil microclimate, which is influenced by site factors including canopy structure, site productivity, and disturbance. However, the extent to which these site factors influence arthropod abundance and diversity, and consequently, spatial variation in Rs is not known. The objective of our …

Using Habituation And Affective Forecasting To Distinguish When The Passive Enjoyment Of Harm Begets The Active Pursuit Of Aggression, Drew M. Parton

Theses and Dissertations

Understanding the antecedents and impellers of aggression is key to preventing violence, and individual differences have been identified as some of the key factors of interest in the study of aggression. Antisocial personality traits such as psychopathy, Machiavellianism, Narcissism, and Sadism have received considerable focus from researchers. Among these traits, Sadism stands above them all as arguably the most malevolent of all trait constructs in psychology. Sadism shows the strongest correlations with aggression among malevolent traits and is uniquely linked to unprovoked aggression, even when it is costly. Sadism thus represents an extreme risk factor for aggression, and studying its …

Scopolamine-Induced Modulation Of Serotonin And Dopamine Transporters Activity: Behavioral And Molecular Insights Into Rapid Antidepressant Action, Deepak Karunakaran

Theses and Dissertations

Major depressive disorder (MDD) affects over 350 million individuals worldwide and is characterized by profound dysregulation of serotonin (5-HT) and dopamine (DA) systems that govern mood, reward processing, and motivation. The serotonin transporter (SERT) and dopamine transporter (DAT), both dynamically regulated through phosphorylation, serve as critical determinants of synaptic monoamine signaling. Despite decades of therapeutic development, current antidepressants require 4-6 weeks to achieve efficacy, and approximately one-third to half of patients develop treatment-resistant depression. The emergence of rapid-acting antidepressants like ketamine represents a paradigmatic shift, demonstrating symptom relief within hours; however, ketamine's therapeutic potential remains constrained by abuse liability, dissociative …

Linking Disturbance & Macrophyte Colonization–Extinction Dynamics To Invasion Success & Community Assembly: Integrating Population, Community, & Landscape Perspectives, Joshua T. Armstrong

Theses and Dissertations

Aquatic plants, macrophytes, are foundational components of freshwater ecosystems, influencing abiotic conditions, providing habitat structure, and shaping biodiversity and community assembly processes. Their role is particularly critical in dynamic systems where disturbance regimes and colonization processes interact to shape community patterns and species distributions. Despite their ecological importance, macrophytes remain underrepresented in theoretical and applied studies of metacommunity dynamics and invasion ecology, especially in natural systems experiencing frequent environmental fluctuations. Here we aim to elucidate how macrophytes influence and respond to spatial and temporal variation in aquatic ecosystems, with a particular focus on colonization-extinction dynamics, invasion success, and community assembly. …

Effectiveness Of Immersive Virtual Reality Combined With Task-Specific Training On Hemiplegic Arm Function Following Stroke: A Single Case Series Study, Rahsaan J. Holley

Theses and Dissertations

Motor recovery of the arm following a neurological injury is a common goal of the rehabilitation process for individuals impacted by the condition . Due to the long-term functional implications of impaired arm movement, individuals are often trained to use compensatory strategies to complete functional tasks without incorporating the weakened limb. While compensatory strategies offer stroke survivors the opportunity to regain a level of independence, such approaches fail to directly address the underlying causes of the disability (i.e., limited range of motion, decreased strength, poor coordination, motor planning difficulties) which can unfortunately result in long-term impairment and functional disability.

Motor …

Identity Formation Of A Pre-Service Art Educator: An Arts-Based Approach To Data Visualization As A Means Of Understanding Personal Experience, Kelsey L. Peterson Mays

Theses and Dissertations

This research centers on the emotional experience of the author as a pre-service art educator during her student teaching semester. The research utilized art-based data collection methods and analyzed both written and visual reflections over an eight-week period of teaching art at the elementary school level. The culmination of findings is synthesized in the form of a body of four visual artworks, where each work reflects the data collected over a consecutive period of two weeks. The study investigated the causation and ramifications of certain emotions during a transitional time wherein the author existed in a liminal space between student …

Characterization Of Outer Surface Protein C Of Borreliella Role In Host Tropism, Kathryn Mf Chambers

Theses and Dissertations

The Lyme disease spirochetes, Borreliella spp., are maintained in nature through a complex enzootic cycle involving Ixodes ticks and a diverse set of reservoir hosts. The outer surface protein C (OspC) of the bacterium is an essential virulence factor required for transmission from ticks and early infection of mammals. Deletion of ospC abolishes infectivity, yet the precise molecular mechanisms underlying OspC function remain incompletely defined. In this dissertation, I examined the contribution of OspC type identity to host specificity. OspC is highly diverse, with over 30 genetically stable types described. While intra-type sequences are conserved (>95% amino acid …

Genetic Studies At The Intersection Of Alcohol Use And Pain, Walker Rogers

Theses and Dissertations

Alcohol use disorder and pain are complex public health concerns affecting millions of people, and these conditions are often characterized by a bidirectional relationship. People experiencing pain may drink alcohol for its analgesic effects. In turn, excessive consumption of alcohol can lead to conditions that cause pain, leading to an escalating feedback loop of pain and alcohol use. Negating this cycle requires a nuanced understanding of the mechanisms underlying alcohol analgesia, which are presently not fully understood. The Miles and Damaj labs have previously shown that ethanol-induced antinociception is moderately heritable in mice, indicating a genetic component to the trait. …

Opioid Tolerance And Withdrawal In The Mouse Colon, James Fune

Theses and Dissertations

Constipation is a major clinical obstacle associated with opioids, prompting patients to discontinue treatment due to pain, discomfort, and psychosocial stressors. Moreover, chronic users become tolerant to the analgesic effects of opioids, but not to their constipating effects. Upon cessation of long-term treatment, serious diarrhea–among other somatic signs of withdrawal–may ensue. These symptoms become a driving factor for relapse, causing patients to restart opioid treatment. In this study, we aim to understand mechanisms that contribute to opioid tolerance and withdrawal in the colon. Previous findings suggest that constipation occurs partly due to the colon’s lack of tolerance to opioids’ inhibitory …

Dickkopf1(Dkk1): A Novel Endogenous Ligand To Prime Nlrp3 Inflammasome Via Tlr4, Theingi Aung

Theses and Dissertations

Dickkopf1(DKK1) is a quintessential Wnt antagonist and immunomodulator in various inflammatory diseases. The underlying molecular mechanisms of DKK1-mediated immunomodulation remain elusive. Here, we identified TLR4 as a new receptor for DKK1 to activate NFκΒ pathway-mediated gene expression and pyroptosis via NLRP3 inflammasome in human and mouse macrophages.

DKK1 employed TLR4 to initiate NFκB signaling cascade via MyD88. MyD88-TAK1-NFκΒ pathway activation by DKK1 increased HIF1α, NFκB, and NLRP3 protein expression levels, leading to pyroptosis. Unlike LPS, DKK1 did not induce IRAK4 phosphorylation, while the interaction between MyD88 and IRAK4 was maintained for downstream signaling activation. DKK1 did not induce IRF3 phosphorylation …

“Some Vague Utopia”: Searching For Urania, Moira L. Neve

Theses and Dissertations

Despite the global pendulum swing of gender trouble marked by the United States’ President’s declaration that there are only two sexes, activists have been rejecting assimilation (ie. defining equality as gay marriage or queer people in the military) within queer studies long before 2025. In the first half of the 20th century, a journal by the name of Urania (1916-1940), privately published and circulated to their home base in the United Kingdom and beyond, five editors (Esther Roper, Eva Gore-Booth, Jessey Wade, Dorothy Cornish, and Irene Clyde/Thomas Baty), challenged the gender binary and the resulting assumptions of heterosexual marriage. This …

(Art) Education In The Afterlife Of Massive Resistance: Constructing, Capturing, And Commodifying Racialized Disability In Virginia’S Student Assignment Practices, 1956-1966 And Present Day, Jenna E. Gabriel

Theses and Dissertations

This dissertation explores discursive functions of disability in pupil placement decisions in Massive Resistance Virginia, the relationship between disability and race that was constructed through these moves, and how these relationships surface in the reflections, perceptions, and pedagogy of a practicing art teacher, Nathan, who works at Arts High School (AHS), a Virginia specialty center high school. Under the umbrella of the Discourse Historical Approach, which relies on multiple, interdisciplinary data sources to explore how discourses evolve, I connect a robust archival analysis of historical data collected in the records of Virginia’s Pupil Placement Board (746 boxes, 1956-1966) with present-day …

Establishing A Conventional Linac-Based Electron Flash Beam, Justin Defrancisco

Theses and Dissertations

Problems: A rediscovered radiotherapy technique, FLASH, involves increasing the dose rate to >40 Gy/s. For a given dose, FLASH is shown to decrease the normal tissue complication probability without affecting the tumor control probability. More preclinical studies are necessary to confirm and enable these desirable properties on humans. Current preclinical investigations have diverse constraints, requiring FLASH apparatuses with flexible parameters and accurate control. However, there are limited FLASH platforms available, the existing ones are costly. Our institution is not in possession of any such infrastructure. Even if available, dosimetry and beam control in FLASH are extremely lacking. There is …

Social Support, Tobacco Attitudes/Perceptions Of Harm, And Asthma Status As Moderators In The Association Between Tobacco Exposure And Cotinine Levels, Rachel L. Pitts

Theses and Dissertations

Tobacco use and exposure are linked to adverse health outcomes (e.g., respiratory conditions) and increased healthcare utilization (e.g., emergency room visits). This study examined the moderating role of social support, tobacco attitudes/perceptions of harm, and asthma status on associations between tobacco use (e.g., combustible product use) and exposure (e.g., secondhand smoke) and cotinine levels among individuals in Richmond, Virginia. We also explored differences in tobacco-related variables by asthma status and associations among tobacco use, secondhand smoke exposure, cotinine levels, and asthma control. Youth (n = 201) and adults (n = 209) (95.7% Black/African American; 60.6% female; 65.8% non-tobacco users; 21% …

Liner Notes On Black Trans Girlhood: Afrofuturist Affect & Black Popular Music As A Praxis Of Self-Fashioning, Shawn J. Williams

Theses and Dissertations

Liner Notes on Black Trans Girlhood: Afrofuturist Affect & Black Popular Music as a Praxis of Self-Fashioning interprets Black musical performance in identity-making, with a focus on how music and Black trans identity intersect and informs Black trans girlhood. As such, Black trans girls render ourselves legible, i.e. understood without shame or judgment, by constructing our identities through the music by Black women we listen and dance to, or watch and sing. These musical connections affirm the possibilities of Black femme expression. This expression of Black femininity is due to what I call an Afrofuturist affect, by which I refer …

Molecular Mechanisms Of Kynurenine-Mediated Lifespan Regulation In Yeast, Ryan E. Counterman Jobb

Theses and Dissertations

The kynurenine pathway is a highly conserved pathway that acts as the primary pathway for tryptophan catabolism and has been found to be involved in lifespan regulation across many diverse organisms. Caloric restriction (CR) is the only known dietary intervention to date that both increases the mean lifespan and the health span of multiple organisms, although the molecular mechanisms of these effects are not fully understood. There are known and sometimes contradictory relationships between the kynurenine pathway activity and the life- extending effect of CR but the crosstalk between the two is not well characterized. Understanding these relationships could lead …

Group Flow Perceptions And Dynamics As Compared To Taxonomy And Integrative Group Flow Theory, Karen E. Wirick

Theses and Dissertations

This qualitative study investigated group flow through a team-building exercise with 18 professionals, examining participants’ and an observer’s perceptions of flow in social contexts. Analysis of interviews and observer notes identified eight themes: Sensory Network of Shared Experience, Adaptive Group Identity and Performance, Goal-Skill-Motivation Alignment, Essential Social Dynamics, Calm Intensity, Synchrony and Engagement Patterns, Spontaneous Collective Celebration, and Observer Integration. These themes revealed that flow in groups manifests as dynamic forces in constant motion. When compared to an emergent Taxonomy and the Integrative Group Flow Theory (IGFT), findings aligned more closely with IGFT's conceptualization of flow as a holistic balancing …

Advancing Human-Computer Interaction Systems Through Explainable And Secure Ai Integration, Ifiok Udoidiok

Theses and Dissertations

As artificial intelligence (AI) systems increasingly shape how humans interact with digital environments, the need for transparency, security, and robustness in intelligent decision making has become critical. This thesis explores how explainable and secure AI techniques can be integrated into modern human-computer interaction (HCI) systems to enhance trust, resilience, and alignment with human operators.

We present three related studies, each addressing a distinct challenge in the design of human-centered AI. First, we apply XAI methods, specifically Local Interpretable Model-Agnostic Explanations (LIME), to deep learning (DL) based CAPTCHA solvers. By interpreting model attention patterns, we uncover exploitable weaknesses in text CAPTCHA …

Inorganic Arsenite [I-As (Iii)] Represses Human Renal Progenitor Cell Characteristics And Induces Neoplastic-Like Transformation, Md Ehsanul Haque

Theses and Dissertations

Studies on populations exposed to inorganic arsenic (iAs) have shown an association between this particular exposure and the development of chronic kidney disease (CKD) and renal cell carcinoma (RCC). However, there are few studies addressing how acute exposure of the human kidney to iAs might lead to long-term alterations that might lead to CKD or RCC. This study’s hypothesis is that renal exposure to iAs might alter the renal cells responsible for the repair and regeneration of nephrons damaged by iAs exposure or other renal toxicants. The kidney possesses a minority epithelial cell population that co-express PROM1 and CD24 that …

Cisplatin-Induced Nephrotoxicity And Adaptive Responses In Renal Progenitor Cells: Molecular Insights From Transcriptomic And Proteomic Analyses, Eloho Ighofose

Theses and Dissertations

Cisplatin is a widely used chemotherapeutic agent known for its efficacy against various solid tumors, but its clinical utility is limited by dose-dependent nephrotoxicity. The renal proximal tubule, particularly its epithelial cell population, is highly susceptible to cisplatin-induced injury due to its role in solute transport and high metabolic activity. Recent advances suggest that renal progenitor cells play a key role in epithelial repair following nephrotoxic injury; however, the molecular mechanisms underlying their response to cisplatin remain poorly understood.This study investigates the impact of both acute and chronic cisplatin exposure on immortalized human renal progenitor cells (HRTPT, CD24⁺/CD133⁺) using a …

The Body Is On, Kitty F. Davies

Theses and Dissertations

THE BODY IS ON is a visual art exhibition that considers queer embodiment, pervert as political identity, the arriving climate catastrophe, and difficulty in artmaking. I make use of various concepts from Deleuze and Guattari to interconnect my own with the primary goal to ask: what does futurity look like or mean in these climates (in both a literal, warming sense and an increasingly fascistic, political one), and how do we actualize that future?