Digital Commons Network^™

Isoprenylation Inhibition Significantly Reduces Ige Mediated Mast Cell Function And Allergic Disease, Jordan Dailey

Theses and Dissertations

Allergic disease is the 6^th leading cause of chronic illness in the US and accounts for billions of dollars in healthcare annually. Mast cells are tissue resident innate immune cells linked to allergic disease and activated by IgE and other ligands. Upon activation, they release histamine, cytokines, chemokines, proteases, and lipid mediators evoking allergic symptoms. New ways of targeting mast cells could greatly benefit allergic disease therapy. Previous findings supported repurposing statin drugs, such as Fluvastatin, as a therapeutic treatment of allergic disease reduced allergic symptoms in vitro and in vivo. We found that Fluvastatin suppressed IgE-mediated mast cell …

Assessing Drug Development Proporties Of Antithrombotic And Anti - Cancer Agents, Elsamani I. Abdelfadiel

Theses and Dissertations

The current GAG anticoagulants such as heparin, heparin derivatives, and vitamin K antagonists, such as warfarin continue to be the backbone of anticoagulant therapy. These drugs act through an indirect mechanism to convey inhibition of several coagulation enzymes. However, xv their use leads to several serious adverse effects, such as excessive bleeding risk and unpredictability of patient response. Regardless of their clinical achievement, every individual agent is accompanied by several side effects, particularly major/minor bleeding, thrombocytopenia, drug-food or drug-drug interactions, or absence of antidote. Of all these side effects, bleeding, and a lack of an effective antidote to reverse excessive …

Molecular Mechanisms Of Prdm16 As A Tumor Suppressor In Pancreatic Ductal Adenocarcinoma, Eric Hurwitz

Theses and Dissertations

The transcription factor Prdm16 functions as a potent suppressor of transforming growth factor-beta (TGF-b) signaling, whose inactivation is deemed essential to the progression of pancreatic ductal adenocarcinoma (PDAC). Using the Kras^G12D-based mouse model of human PDAC, we surprisingly found that ablating Prdm16 did not block but instead accelerated PDAC formation and progression, suggesting that Prdm16 might function as a tumor suppressor in this malignancy. Subsequent genetic experiments showed that ablating Prdm16 along with Smad4 resulted in a shift from a well-differentiated and confined neoplasm to a highly aggressive and metastatic disease, which was associated with a striking deviation …

A Standardized Pipeline For Isolation And Assembly Of Genomes From Symbiotic Bacteria In Whole Louse Genomic Sequence Data., Mohammad Mikail I. Bala

Theses and Dissertations

Many insects are known to harbour intracellular and heritable bacteria (endosymbionts), which provide their hosts with adaptive traits. Whole insect gDNA shotgun sequencing projects often sequence the genome of endosymbiont, in addition to the insect’s genome. There are approximately 600 whole genome shotgun libraries from insects available on the public repository (NCBI), which can be mined to obtain endosymbiont genomes. The assembly and annotation of endosymbiont genomes can contribute towards the exploration of their role as obligate symbiotic partners. However, de novo assembly of an endosymbiont genome, continues to be challenging, when the host and/or enteric bacterial gDNA is present …

Marine And Human Environmental Factors Influence Vibrio Parahaemolyticus And Vibrio Vulnificus In Vitro Biofilm Processes On Plastics, Ryan Eric Leighton

Theses and Dissertations

Marine bacteria often exist in biofilms as communities attached to surfaces, like plastic. Growing concerns exist regarding marine plastics acting as potential vectors of pathogenic Vibrio, especially in a changing climate. It has been generalized that Vibrio vulnificus and Vibrio parahaemolyticus often attach to plastic surfaces. Different strains of these Vibrios exist having different growth and biofilm forming properties. This work evaluated how temperature and strain variability affect V. parahaemolyticus and V. vulnificus biofilm formation and characteristics on glass (GL), low-density polyethylene (LDPE), polypropylene (PP), and polystyrene (PS). This work also evaluated how human body related factors like temperature, …

Oceanographic Applications Of A Monolithic Spatial Heterodyne Raman Spectrometer, Amanda Carol Agrawal

Theses and Dissertations

Carbon dioxide enters the ocean through a variety of natural and anthropogenic sources, and has a significant impact on ocean acidification, the marine buffering system, and bioavailability of life-essential carbonate ions. As carbon dioxide dissolves it reacts with water to form carbonic acid, which then rapidly dissociates into hydronium, bicarbonate, and carbonate ions. Some hydronium ions protonate carbonate to form bicarbonate, driving the carbonate concentration lower while there is a net increase in hydronium concentration. Monitoring the carbonate equilibrium system in the ocean is a complex analytical problem, where speciation is often measured or calculated indirectly ex situ. It …

Producer Interviews And Analysis As A Tool For Science Communication And Research Implementation In Agriculture, Lacy Marie Barnette

Theses and Dissertations

Research implementation beyond the laboratory requires both sound science and high-quality science communication. Science communication is an emerging field that is increasing in importance as the public has access to more information than ever, however there are some underlying obstacles like the lack of communication-specific training that scientists receive and disconnected populations. Agricultural producers have a perceived history of being disconnected from science even though modern agriculture relies heavily on researched practices and has an entire network of extension agencies intended to communicate with producers. One reason for this perceived disconnect is the one-way communication that has dominated science communication …

Evaluating Growth Rates Of The Eastern Oyster (Crassostrea Virginica) And Water Quality For Oyster Restoration Sites At City Island, Bronx, Ny, Cody L. Jernigan

Theses and Dissertations

Oyster growth and water quality were monitored at four sites at City Island, Bronx, NY, to determine suitability for oyster reef restoration. Significant growth occurred at two sites and water quality was not indicated as a contributing factor. Oyster growth at all sites indicate appropriate conditions for oyster reef restoration.

Methods For Drone Trajectory Analysis Of Bottlenose Dolphins (Tursiops Truncatus), Jillian D. Bliss

Theses and Dissertations

With the increase in the use of UAS (Unmanned Aerial Systems) for marine mammal research, there is a need for the development of methods of analysis to transform UAS high resolution video into quantitative data. This study sought to develop a preliminary method of analysis that would quantify and present a way to visualize the dynamics and relative spatial distribution and changes in distribution of bottlenose dolphins (Tursiops truncatus) in the waters of Turneffe Atoll, Belize. This approach employs a previously developed video tracking program ‘Keypoint Tracking’ that enables manual tracking of individual dolphins and the creation of …

Grendel's Mother, Zoe M. Lasden-Lyman

Theses and Dissertations

She helps all the humans and animals in her building. Then why won't she answer this strange, new knocking at her apartment door? A bizarre thriller about the world right now... and about cats.

The Feminization Of Mexico City In The Late Twentieth Century: Polvo De Gallina Negra, Pola Weiss, And Lourdes Grobet, Alexis N. Corral

Theses and Dissertations

This thesis centers on select artworks in public intervention, photography and video as an exploration of female's relationship to Mexico City's social landscape and urban space during the late 1970s into the early 1990s. In three case studies, I explore historical urban planning, gender relations, and the effects of modernization.

Exploring Relationships Between Dog Training Approaches And Aggression, Fear, And Dog-Owner Relationship, Janee Wise

Theses and Dissertations

Abstract

The current study examined relationships between operant conditioning derived dog training techniques and aggression, fear, and dog-owner relationship. Participants (N = 326) completed two online surveys; 1) a survey created for this study measuring the frequency of use of 14 common dog training techniques, which were grouped into four categories based upon operant conditioning: positive reinforcement, negative punishment, positive punishment, and negative reinforcement and 2) the Canine Behavioral Assessment and Research Questionnaire (C-BARQ), which measured several factors related to dog behavior. There were no statistically significant results related to the use of positive reinforcement or positive punishment. However, dog …

Nikki S. Lee’S Self-Stereotyping And Refiguring Cultural Stereotypes, Somi Lee

Theses and Dissertations

This thesis examines a Korean Conceptual photographer, Nikki S. Lee’s performative photographs and film in the series of Projects (1998-2001) and Parts (2002-2005). Through a theoretical analysis of her self-representation in disguise, my research explores established Western stereotypes as well as the artist’s fluid identity in relation to other cultures.

Going Cold Turkey? Changes To Faunal Subsistence In The Northern San Juan Region From Basketmaker Ii Through Pueblo Iii, Tenaya Gatrell-Bedard

Theses and Dissertations

Previous research in the Northern San Juan Region of the American Southwest has revealed a pattern of change in subsistence patterns from the Basketmaker II through Pueblo III periods. Jonathan C. Driver describes the pattern as: cottontail in Basketmaker III (500 to 750 CE) and Pueblo I (750 to 900 CE), deer in Pueblo II (900 to 1150 CE), and turkey in Pueblo III (1150 to 1350 CE). The transition from deer to turkey as the main subsistence has been recorded at several sites throughout the Northern San Juan Region and is thought to be caused by the overhunting of …

Accuracy, Precision, And Efficiency: Comparing Mapping Techniques In Nixtun-Ch’Ich’, Petén, Guatemala, Gabriela Zygadlo

Theses and Dissertations

New archaeological survey technologies have transformed the way in which sites are mapped. Nixtun-Ch’ich’ in Petén, Guatemala has been surveyed in a variety of ways including a theodolite with an electronic distance measurement (EDM), total station (TS), laser imaging, detection and ranging (LiDAR), and photogrammetry. This paper aims to compare various mapping techniques and their accuracy, precision, and efficiency when pertaining specifically to mapping in Nixtun-Ch’ich’, Petén, Guatemala. The goal is to evaluate which technique is most efficient when mapping in the site by considering the variables of cost, time, and environment of the site. The paper also considers the …

Variation In The M-Opioid Receptor (Oprm1) And Offspring Sex Are Associated With Maternal Behavior In Rhesus Macaques (Macaca Mulatta), Zachary Baron

Theses and Dissertations

A m-opioid receptor (OPRM1) single-nucleotide-polymorphism, found in both humans and rhesus macaques mediates the mother-infant attachment bond. Because mothers treat their sons and daughters differently, it is somewhat surprising that the role of infant sex has not been assessed in the context of a maternal-OPRM1-genotype-by-infant-sex interaction. The present study investigates the effect of maternal-OPRM1-genotype and infant sex on mother-infant behaviors. Over the first six months of offspring life, mother-infant behavioral data assessing attachment quality was collected twice weekly from a large number of rhesus monkey mother-infant pairs (N=161 dyads; n=64 female infants, n=97 male infants). Mothers were genotyped for OPRM1 …

The Influence Of Predation Environment On The Sensory Ecology Of Brachyrhaphis Rhabdophora, Alexandra Grace Duffy

Theses and Dissertations

Across the animal kingdom, predation is a ubiquitous and omnipresent selective agent for a variety of traits. I aimed to address gaps in our knowledge pertaining to how predation shapes animal behavior. Many species of fish naturally occur in drainages that differ in the density of predators and exhibit obvious population divergence, making them ideal study organisms to investigate predator-driven behavioral evolution. In Chapter 1, I conducted a systematic review of the literature. The purpose of this review was to determine if predation acted as a stronger or weaker selective agent on particular behavioral traits (e.g., foraging, mating, antipredator etc.) …

Quantifying Volatile Fatty Acid Concentrations During The Pretreatment And Anaerobic Digestion Process, Sascha Boutelle

Theses and Dissertations

Producing renewable energy from biomass or from the wastewater treatment process has gained momentum in the past 20 years. Anaerobic digestion has historically been used as one step in the waste treatment process, to both reduce the mass of waste that needs to be disposed of and to generate biogas. This process is typically only able to reduce 30-40% of the waste mass because of the recalcitrant nature of the waste streams being treated. Anaerobic pretreatment with the thermophilic bacteria Caldicellulosiruptor bescii (C. bescii) has been proven to increase the percentage of mass digested and consequently increase methane production to …

Understanding The Ahupua'a: Using Remote Sensing To Measure Upland Erosion And Evaluate Coral Reef Structure, Logan Kalaiwaipono Ellis

Theses and Dissertations

Under ever intensifying pressures from land use, climate change, and erosion, tropical islands are among the most vulnerable systems in the world. Terrestrial systems are weakened by intensifying land use patterns, the weakening of which is highlighted when high intensity rainfall events erode sediment and leads to sediment deposition on the marine system. The deposition of sediment on the marine system is a major stressor that can lead to weakened coral reefs and a decrease in marine resources commonly gathered for food. These interactions have led to the emergence of biocultural resource management strategies, one of which is the ahupua'a …

Death Spaces And Distant Skies (Every Day We Say Goodbye), Renee Estee

Theses and Dissertations

Linking ideas and imagery of the Australian Outback, Hauntology and Orphism, my practice highlights myth-making and rituals surrounding mourning, celebration and commemoration. Operating as visual love letters and epitaphs, my paintings coalesce figuration and abstraction to conjure spaces where I can commune with the deceased and contend with future loss.

What An Interesting Video To Put On The Internet (An Amusing Economic Indicator), Dahlia S. Bloomstone

Theses and Dissertations

My exhibition reconciles representations of domesticity, labor, and morality through the lens of sex-work (SW). It consists of video work, a video game, and free-to-take objects, where donation, the strip club, and the fish tank converge. My work concludes that SW is a timeless construct that will always exist even after reimagining multiple worlds.

Of Bugs And Wildfires: Tracing The Impacts Of Changing Wildfire Regimes On Aquatic Bacteria And Macroinvertebrates Using Edna, Isabella M. Errigo

Theses and Dissertations

Human disruption of climate, habitat, and ignition has altered the behavior of wildland fire at local to continental scales. In many regions, novel fire regimes are emerging that threaten to exceed the capacity for local management to protect human wellbeing and ecosystem function. Simultaneous changes in climate, species composition, and fire management have resulted in extreme fire behavior in many regions. For the Western United States, the emerging novel fire regime consists of more frequent, severe, and intense wildfires, with annual area burned by wildfire having doubled and high-severity wildfire area having increased 8-fold since the 1980s. The impacts of …

Effect Of Climate History On The Genetic Structure Of An Antarctic Soil Nematode, Abigail C. Jackson

Theses and Dissertations

Historical climate disturbances such as glacial cycling and fluctuating stream, lake, and sea levels strongly influence the distribution and evolutionary trajectories of Antarctic terrestrial species. Antarctic invertebrates, with limited long-range mobility, including the ubiquitous sentinel nematode species Scottnema lindsayae, are especially sensitive to climate disturbances. We tested hypotheses associated with the historical geographic and population genetic structure of this species as it occurs across the McMurdo Dry Valleys (MDVs) of Antarctica. In order to reconstruct the influence of climate disturbance and ecological conditions on this species, partial mitochondrial COI gene sequences were sequenced and analyzed from individual S. lindsayae collected …

Music In The Holocaust: The Roles Of Music In Nazi Concentration Camps, Jennifer L. Lefleur

Theses and Dissertations

The atrocities and horrors of the Holocaust are well-known and well-documented. Hitler’s “Final Solution” was to eradicate all Jews from Europe, and to implement this, the Nazis built six extermination camps in Poland. These camps, and others throughout Europe, mass-killed millions of humans between 1933 and 1945. Prisoners in these camps were consumed by torture, terror, and murder on a daily basis. Although most survivor accounts detail the role music played in these camps, it had been in the last twenty to thirty years that musicologists and historians have really delved into this subject.

While there were different types of …

Core Microbiome To Fingerprint Dust Emission Sources Across The Western United States Of America, Detiare Lisa Leifi

Theses and Dissertations

Over the past century, dust emissions have increased in frequency and intensity due to anthropogenic influences and extended droughts. Dust transports microbes, nutrients, heavy metals and other materials that may then change the biogeochemistry of the receiving environments. The purpose of this study was to find whether unique bacterial communities may provide distinct fingerprints of dust sources in the Western USA. We collaborated with the National Wind Erosion Research Network (NWERN) to identify bacterial core communities (core) of dust from ten NWERN sites, and compared communities to location, soil, and regional characteristics. In order of importance, precipitation levels (F = …

The Effect Of Gut Microbiota On Overwintering Success In Mule Deer, Emma Wilcox

Theses and Dissertations

Mule deer are an important rangeland grazer, large prey species, and game animal for the state of Utah, so herd size is monitored and managed actively. A significant cause of population decline is poor overwintering survival, including from the lack of available forage during winter months. Mule deer energy storage is correlated with greater overwintering success, so physical estimates of energy storage including body condition score (BCS), rump fat (RF), and loin thickness (LT), can be used to track and predict a herd's health. Current methods of collecting deer information are costly, time consuming, and cause physical stress to deer, …

Advanced Isogeometric Discretization Techniques, Kyle Dennis Richardson

Theses and Dissertations

In this dissertation, I provide a robust, efficient inverse mapping algorithm for use in immersed simulation methods, specifically in the Flex Representation Method. I also explore a structural theory that unifies the theories of solids, shells, beams, and rigid bodies. As part of this, I preform a preliminary exploration of applying the Flex Representation Method to shells. Finally, I explore why higher order elements suffer from small critical time steps in explicit dynamics. I then propose a simple method of remedying this issue by exploiting the properties of U-splines.

The Cholera-Fiend: Cheap Fiction, Medical Professionals, And Entertainment, Sariah Fales Harrington

Theses and Dissertations

First published in 1849, Charles Averill’s The Cholera-Fiend follows three villains as they attempt to artificially propagate cholera for their own villainous purposes in New York City. Gumbo, a Black servant to one of the villains, is meant to be the humorous relief in the text, but Gumbo experiences a calculated dehumanization from human to disabled, which causes him to be more at-risk for a health crisis—such as a tapeworm or cholera—than his white counterparts. Through analyzing the genre of cheap fiction, the views of medical professionals towards Black bodies, and other ways Black bodies were used as entertainment, I …

Biofilm Characterization And The Potential Role Of Edna In Horizontal Gene Transfer In Hospital And Meat Isolates Of Staphylococcus Aureus And Their Biofilms, Ashley Lynne Ball

Theses and Dissertations

Staphylococcus aureus is a pathogen responsible for a wide variety of life-threatening diseases such as bacteremia, endocarditis, and pneumoniae. S. aureus has been a major concern in recent years due to the rampant spread of antibiotic resistance. The ability of S. aureus to form biofilms aids in the spread of antibiotic resistance as biofilms are a known hotspot for horizontal gene transfer. Biofilms also protect cells from host immune responses and antibiotics, making these infections very difficult to treat. The matrix of S. aureus biofilms can be made of polysaccharides, protein, and DNA. In these studies, we sought to elucidate …

The Role Of Chitinase A In Mastitis-Associated Escherichia Coli Pathogenesis, Weston D. Hutchison

Theses and Dissertations

Bovine mastitis is a common disease among dairy cattle characterized by the inflammation of the udders and loss of milk production. Mastitis-associated Escherichia coli (MAEC) are frequent causes of the disease, but the features that distinguish them from other E. coli strains remain enigmatic. MAEC infections can range from sub-clinical to severe, acute cases that can be fatal. Historically, the severity of mastitis has been attributed to host factors but more recently, a few bacterial genes have been shown to contribute to virulence in mastitis infections. In a large-scale genomic analysis of >100 MAEC isolates the gene for Chitinase A …