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Role Of Hepatic Cholesterol Ester Hydrolase (Ceh) In Hydrolyzing Cholesterol Esters (Ce) Delivered Via Sr-Bi (Scavenger Receptor Class B Type I) And Sr-Bii, Saurabh Bajpai

Theses and Dissertations

Reduction of cholesterol ester (CE) from lipid burden lesion-associated macrophage foam cells has been shown to reduce plaque volumes. Hydrolysis of CE to free cholesterol (FC) in macrophages is an essential step for removal of CE from the macrophage and its transport to the liver by high density lipoprotein (HDL) for further metabolism. Since CE must again be hydrolyzed into FC in the liver catalyzing this hydrolysis, it becomes imperative to find the identity of these enzymes. In this study the role of key enzyme in catalyzing the hydrolysis of CE to FC, neutral cholesterol ester hydrolase (CEH) was evaluated. …

Steampunk: An Exploration In Design, Christopher Mueller

Theses and Dissertations

The purpose of this thesis is to detail my first professional costume design job after my unofficial graduation in May 2008. This thesis will serve primarily as a design thesis, and will concentrate the majority of its efforts in the presentation of a final, original costume design for a realized production of Sweeney Todd: The Demon Barber of Fleet Street as produced by Duke University. During the course of this presentation, I will seek to detail my own design process and the inspiration and research I used to achieve my goal. I will also discuss the execution of the design, …

Hyperspectral Reflectance For Rapid Viability Assessment Of Bacillus Endospores, Jarrod Edwards

Theses and Dissertations

A study was conducted to optically determine the viability of the Gram +, endospore-forming bacterial genera Bacillus using a hyperspectral reflectance spectrometer. Endospores are a dormant, differentiated cellular capsule form taken by select bacteria to ensure survival when environmental conditions become unfavorable. Two species of Bacillus were used for this study, Bacillus megaterium and Bacillus subtilis. These endospores were killed using a chemical treatment of sodium hypochlorite or a physical treatment of heat and pressure in an autoclave. The treated samples along with viable samples were lyophilized to form a powder. A reflectance spectrometer measuring 350 nm to 2500 nm …

Distribution, Function, And Value Of Parowan Valley Projectile Points, Aaron R. Woods

Theses and Dissertations

This thesis primarily addresses the quantities and distributions of Fremont projectile points in the Parowan Valley. First, I review previous research performed in Parowan Valley and outline currently accepted projectile point analysis and typology methods. I also review ethnographic data surrounding the function and value of projectile points. Then, I provide the results of an analysis of all projectile points in the Parowan Valley Archaeological Project collection. I note the large amount of projectile points in this collection and compare it to projectile point counts from other large Fremont sites.I also note chronological patterns in Parowan Valley using projectile points …

Perceptions Of Spirituality And Perceptions Of God Image: A Qualitative Study, Christopher Elson Peck

Theses and Dissertations

The vast majority of people living in the U.S. report that spirituality is an important part of their life. Ironically, the field of psychology has largely ignored the spiritual beliefs and experiences that so many people see as central to their well-being. Empirical study of spirituality, along with people's differing perceptions of their relationship to God, is a viable area of study for psychologists. This dissertation examines both spirituality and God image and the relationship between these two constructs using qualitative hermeneutic analysis. Findings showed a difficulty defining spirituality among other themes. Findings also showed that individuals' experiences with God …

Paleoindian Lifeways Of Paleoarchaic Peoples: A Faunal Analysis Of Early Occupations At North Creek Shelter, Utah, Bradley A. Newbold

Theses and Dissertations

Recent archaeological research within the American west, especially the Great Basin (e.g., Graf and Schmitt 2007), has perpetuated the notion of decreased residential mobility accompanied by increased diet breadth of hunter-gatherer groups during the Early Holocene. The earliest occupations at North Creek Shelter (NCS), a multicomponent site in south-central Utah, date to this time, specifically the Paleoarchaic (~10,000-9000 BP) and Early Archaic (~9000-7500 BP) periods. The zooarchaeological data from these levels were analyzed to determine whether Paleoarchaic occupations on the Colorado Plateau possessed greater residential mobility and narrower diet breadth than those of the Early Archaic, as they do in …

Multisensory Input To The Lateral Rostral Suprasylvian Sulcus (Lrss) In Ferret, Elizabeth Hagood

Theses and Dissertations

For the brain to construct a comprehensive percept of the sensory world, information from the different senses must converge onto individual neurons within the central nervous system. As a consequence, how these neurons convert convergent sensory input into multisensory information is an important question facing neuroscience today. Recent physiological studies have demonstrated the presence of a robust population of multisensory neurons in the lateral bank of the rostral suprasylvian sulcus (LRSS) in adult ferret (Keniston et al, 2008). The LRSS is a region situated between somatosensory and auditory cortices, where bimodal (somatosensory-auditory) neurons occupy the greatest percentage of the sensory-responsive …

Efficacy Of A Brief Intervention For Insomnia Among Psychiatric Outpatients, James Nile Wagley

Theses and Dissertations

Psychiatric patients are particularly affected by symptoms of insomnia. Because insomnia is often secondary to other conditions and was once thought to be less treatable, this condition has received little attention in terms of treatment and research. Additionally, psychiatric patients have typically fewer resources to seek treatment. Generally, insomnia is treated with medications that may have biological side effects and offer little restorative sleep. Behavioral or cognitive interventions have often been overlooked. This experiment uses profile analysis to test the hypothesis that psychiatric outpatients randomized to a treatment group would have decreased levels of sleep difficulties (measured by PSQI) when …

The Responses Of Blue Crabs (Callinectes Sapidus) To Hypoxia/Hypercapnia In Freshwater, James Martin

Theses and Dissertations

The present research examined respiratory responses of blue crabs to long term (4, 13, and 21 days) hypercapnic hypoxia in freshwater at 23 C. Hypoxic conditions (50-60 & 75-85 mmHg O2) were induced by allowing the crabs to consume their oxygen supply, resulting in a hypercapnic induced decrease in pH that remained through the exposure. Postbranchial hemolymph responses to hypoxia/hypercapnia in freshwater demonstrate decreases in PO2, increases in PCO2, and decreases in pH. Lactate levels decreased over time, but hemocyanin concentration was highly variable with no trends. PH, lactate, and hemocyanin observations also demonstrated high variability and a variety of …

The Presence Of Amphibian Ranavirus In Virginia Warm Water Fish Hatchery Ponds, Elizabeth Nelson

Theses and Dissertations

Amphibian declines have been occurring world wide over the past several decades. Infectious disease and environmental changes have been implicated as the causative agents in these declines. Fish stocking from hatcheries provides a unique opportunity for organisms, including infectious diseases, to travel over long distances. Ranavirus was previously found in two ponds at Harrison Lake National Fish Hatchery in Charles City County, Virginia. Therefore, the primary objective of this study was to determine if ranavirus is present in tadpoles from four warm water fish hatcheries in Virginia. A secondary objective of this study was to determine if there are relationships …

The Role Of Connexin-36 Gap Junctions In Alcohol Intoxication And Reward, Kathryn Diane Bradley

Theses and Dissertations

The purpose of this thesis project was to examine the function of connexin-36 (Cx36) gap junctions (GJs) in producing alcohol's intoxicating and rewarding effects. GABA neurons are thought to inhibit dopamine (DA) neurotransmission in the mesocorticolimbic system, which originates in the midbrain ventral tegmental area (VTA) and projects to limbic structures such as the nucleus accumbens (NAcc). The mesolimbic DA system is believed to be the neural substrate of alcohol intoxication and addiction (Tepper, Paladini, & Celada, 1998). Alcohol suppresses the firing rate of GABA neurons in the VTA (Gallegos, Criado, Lee, Henriksen, & Steffensen, 1999) and presumably disinhibits DA …

Molecular Systematics Of Spiny Pocket Mice (Subfamily Heteromyinae) Inferred From Mitochondrial And Nuclear Sequence Data, Melina Crystal Williamson

Theses and Dissertations

This study aims to determine species-level relationships within the genus Heteromys, as well as generic-level relationships among members of the subfamily Heteromyinae using a phylogenetic framework. Molecular sequence data were generated from two mitochondrial genes (cytochrome b and cytochrome oxidase I) and three nuclear gene segments (β-fibrinogen, engrailed protein II, and myosin heavy chain II), and analyzed under maximum parsimony, maximum likelihood, and Bayesian optimality criteria to infer relationships. Chapter 1 focuses on the phylogenetic and taxonomic implications for Heteromys from the analyses of sequence data. Phylogenies also provided a framework for delimiting species boundaries within the wide-ranging Heteromys desmarestianus …

Matrix Metalloproteinase Expression In Models Of Parkinson's Disease, Justin Mcclain

Theses and Dissertations

Parkinson’s disease (PD) is a devastating neurodegenerative disorder characterized by progressive loss of dopaminergic neurons located in the substantia nigra. Accumulating evidence indicates that microglia-driven neuroinflammation contributes significantly to chronic neurodegeneration in PD. The matrix metalloproteinases (MMPs) play an important role in several neuroinflammatory paradigms; however, their relationship to dopaminergic neurodegeneration in PD remains relatively unexplored. To address this, the temporal relationship between MMP-2, MMP-3, MMP-9, and MMP-13 expression and dopaminergic neurodegeneration was compared in the 6-hydroxydopamine (6-OHDA) and lipopolysaccharide (LPS) models of PD. In dopaminergic SH-SY5Y cells, 6-OHDA treatment significantly increased MMP-13 mRNA expression; however, examination of 6-OHDA-lesioned rats …

The Roberta Jones Junior Theatre: A Model Children's Theatre, Chareen Hardy Lauritzen

Theses and Dissertations

Since its beginning in 1968, the Roberta Jones Junior Theatre, a children's theatre, connected to the Parks and Recreation Department of Santa Clara, California, has been creating child-centered performances for young audiences. This thesis will explore the history of the Santa Clara/Roberta Jones Junior Theatre and discuss the components that have contributed to its forty-year success. For a children's theatre to be successful it needs to be first centered on the learning of the children, in tune with the need of its community, and have a staff focused on the same goals. By examining the Roberta Jones Junior Theatre, the …

The Role Of Myeloid-Derived Suppressor Cells In The Immunotherapy Of Breast Carcinomas, Johanna Morales

Theses and Dissertations

Myeloid-derived suppressor cells (MDSC) are a heterogeneous population of immature cells at various stages of differentiation. These cells are broadly characterized by the simultaneous expression of the surface markers CD11b and Gr1 and have been found to accumulate in large numbers in response to many different tumors in both mice and humans, including HER2/neu+ breast cancers. The adoptive immunotherapy of cancers has been a promising field, yet the clinical efficacy of adoptive immunotherapies targeted against human breast cancers and many other cancers has been extremely limited. Given the influx of MDSC in tumor-bearing individuals, we hypothesized that these cells were …

The Role Of Mitogen-Activated Protein Kinases In The Regulation Of Plant Development, Erica Satterfield

Theses and Dissertations

Mitogen-activated protein kinases are part of an evolutionarily conserved protein phosphorylation cascade which serves essential regulatory functions in eukaryotic organisms. Although the role of MAPKs in the regulation of a plant’s response to environmental stress and plant defense has been well established, very little is known about their role in the regulation of plant developmental processes. In order to examine the role of MAPKs in plant growth and development, a strong mammalian MAPK phosphatase (MKP-1), which is known to inactivate MAPKs in plants, was introduced into tobacco plants. In tobacco plants, MKP-1 overexpression altered plant responses to the phytohormones, ethylene …

Cannabinoid Effects On Nfkappab Function In Microglial-Like Cells: Dual Mode Of Action, Latoya Griffin-Thomas

Theses and Dissertations

Cannabinoids have been shown to modulate the immune system in vitro and in animal models. A major area of interest is how cannabinoids impact the brain. A whole variety of neuropathies or brain disorders, such as AIDS dementia, Parkinson’s disease, Multiple Sclerosis and Alzheimer’s disease, are associated with a hyperinflammatory response within the brain. Microglia, the resident macrophages of the brain, are the major cell type responsible for the persistent elicitation of pro-inflammatory cytokines (IL-1a, IL-1b, IL-6, TNFa) and other mediators. In vitro experiments have demonstrated that the partial exogenous cannabinoid agonist delta-9-tetrahydrocannabinol (D9-THC) and the potent synthetic exogenous cannabinoid …

"Splintered And A Great Silence": The Production Of Return To The Upright Position By Caridad Svich, Catherine (Cate) Brewer

Theses and Dissertations

Like the Tribute in Light that grew phoenix-like from the ashes of the broken hearts of the artists who created it, Return to the Upright Position is a beautiful on-line collaboration amongst fourteen artists in the six months following 9/11. Conceived and edited by Caridad Svich, the play is moving and delicate and instantly brings us back to those moments when American history and sentiment were forever changed. Demoralized, terrorized, afraid, and alone, our country dragged its shocked and weary carcass from the ash, and pulled itself back up again. That is after all the American way, pulling yourself up …

A Beginning Teacher's Guide To Beginner Violinists, Anjuli Coe

Theses and Dissertations

In the following pages, the beginning teacher will find a guide to establishing a studio and ideas for teaching students. These ideas are meant to be a springboard for developing personalized organization and teaching tools for the violin studio. Ability to play the violin is not the only ingredient for a successful violin studio. Teaching the violin requires skill in understanding students and how they learn. It also requires a significant amount of planning, preparation, organization, and the setting of goals and objectives.

Beginner violinists may vary from a four-year-old with highly involved parents, to a professional trumpet player who …

Comparative Ecology And Conservation Status Of Procambarus (Pennides) Gibbus And Procambarus (Pennides) Versutus In Marion County, Georgia, Joann Chadwick

Theses and Dissertations

Two crayfish species, whose conservation status was of concern, Procambarus (Pennides) gibbus Hobbs and Procambarus (Pennides) versutus Hagen, and one relatively common crayfish, Procambarus (Pennides) spiculifer LeConte were studied at nine creek sites in Marion County, Georgia. Major objectives of the yearlong study were to 1) compare the habitats of these species, looking for environmental differences that might account for their isolated distributions within the county and 2) assess the current conservation status of P. gibbus and P. versutus. Each study site was characterized by a one-time assessment of floodplain width, stream width, water velocity, stream depth and assessment of …

Neurotensin Regulates Gabaergic Activity In The Ca1 Region Of Rat Hippocampus, Shanshan Li

Theses and Dissertations

Neurotensin (NT) is a tridecapeptide that interacts with three NT receptors, NTS1, NTS2 and NTS3. NT has been reported to modulate GABA release in various brain regions under both physiological and pathological conditions. A beneficial role for NT in correcting GABA dysfunction/deficiency has been implied. However, the cellular and molecular mechanisms underlying NT-induced increases in GABAergic function remain ill-defined. Here, we first examined the effects of NT on GABAergic transmission and the underlying cellular and signaling mechanisms in the hippocampus of juvenile rats. We found that NT dose-dependently increased sIPSC frequency in CA1 pyramidal neurons without affecting the amplitude of …

The Role Of Src Suppressed C-Kinase Substrate In Primary Sensory Neurons, Christopher P. Irmen

Theses and Dissertations

SSeCKS (src suppressed C kinase substrate) is an A kinase anchoring protein that has been localized in the peripheral and central nervous systems. Previous studies have suggested that SSeCKS is restricted to a population of small-diameter neurons in the dorsal root ganglia whose central axons terminate in superficial laminae of the spinal cord dorsal horn. This SSeCKS containing population displayed labeling characteristics consistent with non-peptidergic C-fibers and occasionally contained nerve growth factor receptors. The present study investigated the hypothesis that SSeCKS is found in a subgroup of primary sensory neurons that are involved in transmission of nociceptive stimuli. This was …

Speaking Of Myself: Independence, Self-Representation, And The Speeches Of Rudyard Kipling, Jacob M. Wilkes

Theses and Dissertations

Rudyard Kipling is a man of immense diversity. He successfully managed to write for over half a century in a variety of genres: short story, travelogue, ballad, personal narrative, and news reporting, to name only a few. While doing so, Kipling readily interacted with a range of subjects and created a multitude of ideas. Likewise, on a personal level, Kipling led an immensely diverse life. He could easily claim four separate continents as home, living variously in India, the United States, England, and South Africa. By profession he was a writer, but as an observer he was so skilled that …

Evaluating The Influence Of Past Gaming Experience On Learner Preferences And Motivation To Learn In A Military Training Environment, Steven D. Yelverton

Theses and Dissertations

Millennial generation recruits are entering the Air Force and with them come their new attitudes and expectations. As a result, the leadership of the Air Education and Training Command (AETC) expressed a need for information on how to effectively train this new generation of recruits in their 2008 whitepaper. The purpose of this thesis was to begin to address this requirement by investigating the influence video gaming has on the learning preferences of trainees undergoing initial skills training at the 82nd Training Wing, Sheppard Air Force Base, Texas. A survey was administered and data was collected from 866 trainees. The …

The Emergence Of A Content Acceptance Model (Cam): New Thoughts Regarding The Trial, Adoption, And Usage Of New Media, Ryan G. Walinski

Theses and Dissertations

New Media is defined not by the technology that it is based on but rather on individual level attributes that contribute to the development of new artifacts, practices and social arrangements. However, existing technology adoption models tend to stress technology and organizational level attributes over such individual level characteristics. This suggests that new models are required in order to fully capture how the New Media adoption process works. We are moving away from organizational and technology adoption focus and towards an individual and content adoption focus. A review of existing technology acceptance models reveals that the content provided by these …

Exploitation Of Geographic Information Systems For Vehicular Destination Prediction, Richard T. Muster

Theses and Dissertations

Much of the recent successes in the Iraqi theater have been achieved with the aid of technology so advanced that celebrated journalist Bob Woodward recently compared it to the Manhattan Project of WWII. Intelligence, Surveillance, and Reconnaissance (ISR) platforms have emerged as the rising star of Air Force operational capabilities as they are enablers in the quest to track and disrupt terrorist and insurgent forces. This thesis argues that ISR systems have been severely under-exploited. The proposals herein seek to improve the machine-human interface of current ISR systems such that a predictive battle-space awareness may be achieved, leading to shorter …

Functionalization, Characterization, And Applications Of Diamond Particles, Modification Of Planar Silicon, And Chemoetrics Analysis Of Ms Data, Li Yang

Theses and Dissertations

In spite of the stablility (lack of reactivity) of diamond powder, I have developed a method for tethering alkyl chains and polymers to deuterium/hydrogen-terminated diamond. One method is through ether linkages via thermolysis of di-tert-amyl peroxide (DTAP). This reaction with DTAP has also been applied to grow polymers on the diamond surface. The other method is atom transfer radical polymerization (ATRP), which was applied to grow polystyrene at the surface of diamond. Both polystyrene-modified diamond and sulfonated polystyrene-modified diamond can be prepared by either method, and can be used for solid phase extraction. Diamond stationary phases are stable under basic …

The Effect Of Simulated Nodules On Vocal Fold Movement In A Two Layer Synthetic Model, Rachelle Nevitt Rauma

Theses and Dissertations

This study examined the differences between normal vocal fold vibration and the movement patterns of vocal folds with mass lesions by means of a synthetic model. The experimenter molded and cast three sets of vocal folds, representing normal structure, small nodules, and larger nodules. Acoustic, aerodynamic, and digital video signals were recorded and analyzed in order to quantify air flow and pressure, measure vibratory stability, and visually assess closure patterns across the three structural conditions. Statistical analysis revealed that the presence of vocal nodules resulted in a significantly higher onset pressure, fundamental frequency, airflow at onset, and offset pressure. However, …

Posttraumatic Stress Disorder: A Theory Of An Efficacious Treatment Plan., Valerie Jeanette Johnston

Theses and Dissertations

There are numerous commonalities and similarities in the symptoms presented in those individuals suffering from Korsakoff's Syndrome, Vascular Dementia, B vitamin deficiencies and Posttraumatic Stress Disorder. These similarities include physiological, neurological and psychological findings. B vitamin supplementation is being used effectively to treat several of these disorders. Because of these similarities and commonalities in both symptomology and physiology, it is my hypothesis that PTSD may be partially or totally a consequence of a B vitamin deficiency. Narrative re-exposure therapy is already in use to treat those with PTSD. Using the Biopsychosocial Model of health psychology, I propose an empirical study …

Twenty Teaching Proficiency Through Reading And Storytelling (Tprs) Lessons For The Spanish I Classroom, Anne Marie Taulbee

Theses and Dissertations

With the emergence of new teaching methods in foreign language such TPRS (Teaching Proficiency through Reading and Storytelling, formerly Total Physical Response Storytelling), teachers are faced with the problem of implementing this method into the classroom where it is often met with sceptisim from collegues and admistrators. By using TPRS, teachers focus on providing comprehensible input in a "top down" model. By using elements of both methods, namely storytelling for vocabulary and grammatical structures as well as discreet grammar explanation, teachers can use both methods in a way that is effective. The focus of this thesis is to provide teachers …