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Novel In Silico Method For Teaching Cytoarchitecture, Cellular Diversity, And Gene Expression In The Mammalian Brain, Raddy L. Ramos, Phoebe T. Smith, Joshua C. Brumberg

Publications and Research

Neuroanatomy can be a challenging topic for undergraduates, making the development of new methods of instruction an important goal of neuroscience educators. In the present report we describe the utility and versatility of the Allen Brain Atlas as a novel tool for instruction of several important anatomical principles of the mammalian central nervous system. Using this digital database, we detail how instructors of laboratory or lecture-based courses can demonstrate cytoarchitecture, cellular diversity, and gene expression profiles of the brain.

Northeast Under/Graduate Research Organization For Neuroscience (Neuron): Our Third New York City Meeting, Sharon Ramos Goyette, Kassandra L. Edinger, Vicki Luine, Jason Young, Cheryl A. Frye

Publications and Research

The NorthEast Under/graduate Research Organization for Neuroscience (N.E.U.R.O.N.) promotes preparation, education, and undergraduate research in Neuroscience. The N.E.U.R.O.N. Conference was initially held at undergraduate institutions primarily in New England. Then, for the previous two years, to broaden its impact and increase diversity, the meeting moved to Hunter College, CUNY, New York. This year represents the first year in which two N.E.U.R.O.N. meetings were held, one in Boston and one in New York City. The following is a report of the New York City meeting which was held at Hunter College on April 28, 2007. Eminent Neuroscientist, Dr. Carol Sue Carter, …

Growth Environment And Sex Differences In Lipids, Bodyshape And Diabetes Risk, Mary Schooling, Tai Hing Lam, G. Neil Thomas, Benjamin J. Cowling, Michelle Heys, Edward D. Janus, Gabriel M. Leung

Publications and Research

Background
Sex differences in lipids and body shape, but not diabetes, increase at puberty. Hong Kong Chinese are mainly first or second generation migrants from China, who have shared an economically developed environment for years, but grew up in very different environments in Hong Kong or contemporaneously undeveloped Guangdong, China. We assessed if environment during growth had sex-specific associations with lipids and body shape, but not diabetes.

Methodology and Principal Findings
We used multivariable regression in a population-based cross-sectional study, undertaken from 1994 to 1996, of 2537 Hong Kong Chinese residents aged 25 to 74 years with clinical measurements of …

Perigenual Anterior Cingulate Morphology Covaries With Perceived Social Standing, Peter J. Gianaros, Jeffrey A. Horenstein, Sheldon Cohen, Karen A. Matthews, Sarah M. Brown, Janine D. Flory, Hugo D. Critchley, Stephen B. Manuck, Ahmad R. Hariri

Publications and Research

Low socioeconomic status (SES) increases the risk for developing psychiatric and chronic medical disorders. A stress-related pathway by which low SES may affect mental and physical health is through the perception of holding a low social standing, termed low subjective social status. This proposal implicates overlapping brain regions mediating stress reactivity and socioemotional behaviors as neuroanatomical substrates that could plausibly link subjective social status to health-related outcomes. In a test of this proposal, we used a computational structural neuroimaging method (voxel-based morphometry) in a healthy community sample to examine the relationships between reports of subjective social status and regional gray …

Nectin-Like Proteins Mediate Axon–Schwann Cell Interactions Along The Internode And Are Essential For Myelination, Patrice Maurel, Steven Einheber, Jolanta Galinska, Pratik Thaker, Isabel Lam, Marina B. Rubin, Steven S. Scherer, Yoshinuri Murakami, David H. Gutmann, James L. Salzer

Publications and Research

Axon–glial interactions are critical for the induction of myelination and the domain organization of myelinated fibers. Although molecular complexes that mediate these interactions in the nodal region are known, their counterparts along the internode are poorly defined. We report that neurons and Schwann cells express distinct sets of nectin-like (Necl) proteins: axons highly express Necl-1 and -2, whereas Schwann cells express Necl-4 and lower amounts of Necl-2. These proteins are strikingly localized to the internode, where Necl-1 and -2 on the axon are directly apposed by Necl-4 on the Schwann cell; all three proteins are also enriched at Schmidt-Lanterman incisures. …

Evolution Of Dispersal And Life History Strategies – Tetrahymena Ciliates, Else J. Fjerdingstad, Nicolas Schtickzelle, Pauline Manhes, Arnaud Gutierrez, Jean Clobert

Publications and Research

Background Considerable attention has focused on how selection on dispersal and other core life-history strategies (reproductive effort, survival ability, colonization capacity) may lead to so-called dispersal syndromes. Studies on genetic variation in these syndromes within species could importantly increase our understanding of their evolution, by revealing whether traits co-vary across genetic lineages in the manner predicted by theoretical models, and by stimulating further hypotheses for experimental testing. Yet such studies remain scarce. Here we studied the ciliated protist Tetrahymena thermophila, a particularly interesting organism due to cells being able to transform into morphs differing dramatically in swim-speed. We investigated dispersal, …

Novel Research Translates To Clinical Cases Of Schizophrenic And Cocaine Psychosis, João V. Nunes, Patricia A. Broderick

Publications and Research

Pharmacotherapies for schizophrenic and cocaine psychoses are complex but similar because of similarities in their brain neurochemistry and behavioral outcomes. Their neurochemical neuronal mechanisms of action, as shown in preclinical and clinical studies, involve primarily dopaminergic dysfunction and, secondarily, neuroadaptive effects that seem to involve central serotonergic function. Behavioral outcomes of both disorders include hyperactivity and antipsychotic medications can ameliorate psychotic symptoms. Patients with both disorders often arrive at emergency departments and present floridly psychotic with a predominance of positive symptoms, often prompting physicians to select a typical antipsychotic medication such as haloperidol. While this has become conventional wisdom, we …

Prediction Of Cyclin-Dependent Kinase Phosphorylation Substrates, Emmanuel J. Chang, Rashida Begum, Brian T. Chait, Terry Gaasterland

Publications and Research

Protein phosphorylation, mediated by a family of enzymes called cyclin-dependent kinases (Cdks), plays a central role in the cell-division cycle of eukaryotes. Phosphorylation by Cdks directs the cell cycle by modifying the function of regulators of key processes such as DNA replication and mitotic progression. Here, we present a novel computational procedure to predict substrates of the cyclin-dependent kinase Cdc28 (Cdk1) in the Saccharomyces cerevisiae. Currently, most computational phosphorylation site prediction procedures focus solely on local sequence characteristics. In the present procedure, we model Cdk substrates based on both local and global characteristics of the substrates. Thus, we define the …

Human Mst But Not Mt Responds To Tactile Stimulation, Michael S. Beauchamp, Nafi E. Yasar, Neel Kishan, Tony Ro

Publications and Research

Previous reports of tactile responses in human visual area MT/V5 have used complex stimuli, such as a brush stroking the arm. These complex moving stimuli are likely to induce imagery of visual motion, which is known to be a powerful activator of MT. The area described as “MT” in previous reports consists of at least two distinct cortical areas, MT and MST. Using functional magnetic resonance imaging, we separately localized human MT and MST and measured their response to vibrotactile stimuli unlikely to induce imagery of visual motion. Strong vibrotactile responses were observed in MST but not in MT. Vibrotactile …

Comparative Mitochondrial Genomics Of Snakes: Extraordinary Substitution Rate Dynamics And Functionality Of The Duplicate Control Region, Zhi J. Jiang, Todd A. Castoe, Christopher C. Austin, Frank T. Burbrink, Matthew D. Herron, Jimmy A. Mcguire, Christopher L. Parkinson, David D. Pollock

Publications and Research

Background The mitochondrial genomes of snakes are characterized by an overall evolutionary rate that appears to be one of the most accelerated among vertebrates. They also possess other unusual features, including short tRNAs and other genes, and a duplicated control region that has been stably maintained since it originated more than 70 million years ago. Here, we provide a detailed analysis of evolutionary dynamics in snake mitochondrial genomes to better understand the basis of these extreme characteristics, and to explore the relationship between mitochondrial genome molecular evolution, genome architecture, and molecular function. We sequenced complete mitochondrial genomes from Slowinski's corn …

Msbayes: Pipeline For Testing Comparative Phylogeographic Histories Using Hierarchical Approximate Bayesian Computation, Michael J. Hickerson, Eli Stahl, Naoki Takebayashi

Publications and Research

Background Although testing for simultaneous divergence (vicariance) across different population-pairs that span the same barrier to gene flow is of central importance to evolutionary biology, researchers often equate the gene tree and population/species tree thereby ignoring stochastic coalescent variance in their conclusions of temporal incongruence. In contrast to other available phylogeographic software packages, msBayes is the only one that analyses data from multiple species/population pairs under a hierarchical model. Results msBayes employs approximate Bayesian computation (ABC) under a hierarchical coalescent model to test for simultaneous divergence (TSD) in multiple co-distributed population-pairs. Simultaneous isolation is tested by estimating three hyper-parameters that …

Maintaining Phase Of The Tri-Phasic Crab Pyloric Rhythm, Christina Mouser, Farzan Nadim, Amitabha Bose

Publications and Research

No abstract provided.

Violence, Vision, And Voice: A Journey From Liminal To Transgressive Spaces, Stephanie Urso Spina

Publications and Research

This paper was inspired by the author’s experiences teaching a required class about feminism to affluent, predominantly female undergraduates who vociferously considered it outdated and irrelevant to their lives until they realized, in painfully personal ways, that this was the dominant discourse speaking, not their own voices. Inspired by these women, and in the hope of further displacing the hegemonically imposed code of silence, this paper breaks the author’s tacit complicity with these societal forces of repression. Written on a bus from Boston to New York, the author weaves her narrative with a description of that trip and its passengers, …

Utility And Versatility Of Extracellular Recordings From The Cockroach For Neurophysiological Instruction And Demonstration, Raddy L. Ramos, Andrew Moiseff, Joshua C. Brumberg

Publications and Research

The principles of neurophysiology continue to be challenging topics to teach in the context of undergraduate neuroscience education. Laboratory classes containing neurophysiological demonstrations and exercises are, therefore, an important and necessary complement for covering those subjects taught in lecture-based courses. We developed a number of simple yet very instructive exercises, described below, which make use of extracellular recordings from different sensory systems of the cockroach (Periplanta americana). The compendium we developed provides students with hands-on demonstrations of several commonly taught topics of neurophysiology including sensory coding by neural activity.

Genetic Randomization Reveals Functional Relationships Among Morphologic And Tissue-Quality Traits That Contribute To Bone Strength And Fragility, Karl J. Jepsen, Bin Hu, Steven M. Tommasini, Hayden-William Courtland, Christopher Price, Carl J. Terranova, Joseph H. Nadeau

Publications and Research

We examined femora from adult AXB/BXA recombinant inbred (RI) mouse strains to identify skeletal traits that are functionally related and to determine how functional interactions among these traits contribute to genetic variability in whole-bone stiffness, strength, and toughness. Randomization of A/J and C57BL/6J genomic regions resulted in each adult male and female RI strain building mechanically functional femora by assembling unique sets of morphologic and tissue-quality traits. A correlation analysis was conducted using the mean trait values for each RI strain. A third of the 66 correlations examined were significant, indicating that many bone traits covaried or were functionally related. …

Bio::Nexus: A Perl Api For The Nexus Format For Comparative Biological Data, Thomas Hladish, Vivek Gopalan, Chengzhi Liang, Weigang Qiu, Peter Yang, Arlin Stoltzfus

Publications and Research

Background Evolutionary analysis provides a formal framework for comparative analysis of genomic and other data. In evolutionary analysis, observed data are treated as the terminal states of characters that have evolved (via transitions between states) along the branches of a tree. The NEXUS standard of Maddison, et al. (1997; Syst. Biol. 46: 590–621) provides a portable, expressive and flexible text format for representing character-state data and trees. However, due to its complexity, NEXUS is not well supported by software and is not easily accessible to bioinformatics users and developers. Results Bio::NEXUS is an application programming interface (API) implemented in Perl, …

A Reduction Algorithm For Computing The Hybridization Number Of Two Trees, Magnus Bordewich, Simone Linz, Katherine St. John, Charles Semple

Publications and Research

Hybridization is an important evolutionary process for many groups of species. Thus, conflicting signals in a data set may not be the result of sampling or modeling errors, but due to the fact that hybridization has played a significant role in the evolutionary history of the species under consideration. Assuming that the initial set of gene trees is correct, a basic problem for biologists is to compute this minimum number of hybridization events to explain this set. In this paper, we describe a new reduction-based algorithm for computing the minimum number, when the initial data set consists of two trees. …

Behavioral Approaches To Weight Control: A Review Of Current Research, Angela Marinilli Pinto, Jessica Gokee-Larose, Rena R. Wing

Publications and Research

Weight management is a salient issue for women. Studies of behavioral, pharmacological and surgical interventions indicate that women comprise the majority of patients presenting for weight-loss treatment. In this review we discuss the health impact of obesity for women, review behavioral treatments for adult overweight and obesity, and address topics of particular relevance for women, including concerns that weight-loss treatment may precipitate the development of eating pathology, as well as time periods of high risk for weight gain such as pregnancy and menopause.

Low-Income Minority Mothers’ Reports Of Infant Health Care Utilisation Compared With Medical Records, Kennan D. Murray, Ayman El-Mohandes, M. Nabil El-Khorazaty, Michele Kiely

Publications and Research

This study aimed to investigate mothers’ reporting of the nature, location, frequency and content of health care visits for their infants, as compared with data abstracted from the infants’ medical records. It was part of a community-based parenting intervention designed to improve preventive health care utilisation among minority mothers in Washington, DC. Mothers 18 years old with newborn infants and with poor or no prenatal care were enrolled in the study. A total of 160 mother–infant dyads completed the 12-month study. Mothers were interviewed when the infants were 4, 8 and 12 months old, and were asked to recall infant …

A Role For Sperm In Regulation Of Egg-Laying In The Nematode C. Elegans, Marie Mcgovern, Ling Yu, Mary Kosinski, David Greenstein, Cathy Savage-Dunn

Publications and Research

Background In insects and in mammals, male sperm and seminal fluid provide signaling factors that influence various aspects of female physiology and behavior to promote reproductive success and to compete with other males. It is less apparent how important such signaling is in the context of a self-fertile hermaphrodite species. We have addressed this question in the nematode Caenorhabditis elegans, which can reproduce either by hermaphrodite self-fertilization or by male-hermaphrodite mating.

Results We have studied the egg-laying defective mutant, egl-32, and found that the cellular basis of the egl-32 egg-laying phenotype is likely a defect in sperm. First, the time …

Introduction: The Sexual Body, Shelly J. Eversley, Jennifer L. Morgan

Publications and Research

Introduction to the special issue, "The Sexual Body," edited by Shelly Eversley and Jennifer L. Morgan.

Female Agency And Oppression In Caribbean Bacchanalian Culture: Soca, Carnival, And Dancehall, Kevin Frank

Publications and Research

In this essay Kevin Frank discerningly analyzes agency and gender in public sexual performances emanating out of what Paul Gilroy identifies as part of the compensatory politics of the subordinated within Black Atlantic culture, Jamaican dancehall (dancehall reggae/ dancehall queens).

Inhibition Of Caveolar Uptake, Sv40 Infection, And Β1-Integrin Signaling By A Nonnatural Glycosphingolipid Stereoisomer, Raman Deep Singh, Eileen L. Holicky, Zhi-Jie Cheng, Seong-Youl Kim, Christine L. Wheatley, David L. Marks, Robert Bittman, Richard E. Pagano

Publications and Research

Caveolar endocytosis is an important mechanism for the uptake of certain pathogens and toxins and also plays a role in the internalization of some plasma membrane (PM) lipids and proteins. However, the regulation of caveolar endocytosis is not well understood. We previously demonstrated that caveolar endocytosis and β1-integrin signaling are stimulated by exogenous glycosphingolipids (GSLs). In this study, we show that a synthetic GSL with nonnatural stereochemistry, β-d-lactosyl-N-octanoyl-l-threo-sphingosine, (1) selectively inhibits caveolar endocytosis and SV40 virus infection, (2) blocks the clustering of lipids and proteins into GSLs and cholesterol-enriched microdomains (rafts) at the PM, and (3) inhibits β1-integrin activation and …

Secular Trends In The Association Of Socio-Economic Position With Self-Reported Dietary Attributes And Biomarkers In The Us Population: National Health And Nutrition Examination Survey (Nhanes) 1971–1975 To Nhanes 1999–2002, Ashima K. Kant, Barry I. Graubard

Publications and Research

Objective: Recent reports suggest persistence of health disparities related to socioeconomic position (SEP). To understand if diet may be a contributor to these trends, we examined secular trends in the association of diet and indicators of SEP from 1971–1975 to 1999–2002.

Design: We used data from the National Health and Nutrition Examination Surveys (NHANES) I (1971–1975), II (1976–1980), III (1988–1994) and 1999–2002 to examine the independent associations of poverty income ratio (PIR) and education with diet and biomarkers of diet and disease in 25–74-year-olds (n ¼ 36 600). We used logistic and linear regression methods to adjust for multiple covariates …

Maintenance Of Visual Stability In The Human Posterior Parietal Cortex, Erik Chang, Tony Ro

Publications and Research

Visual stability refers to our stable visuospatial perceptions despite the unstable visual input caused by saccades. Functional neuroimaging results, studies on patients with posterior parietal cortex (PPC) lesions, and single-unit recordings in the lateral intraparietal sulcus of primates indirectly suggest that the PPC might be a potential locus of visual stability through its involvement with spatial remapping. Here we directly explored the role of the PPC in visual stability by applying transcranial magnetic stimulation (TMS) while participants performed a perisaccadic displacement detection task. We show that TMS over the PPC but not a frontal control site alters sensitivity to displacement …

An Equal Protection Standard For National Origin Subclassifications: The Context That Matters, Jenny Rivera

Publications and Research

No abstract provided.

Transnational Operations, Bi-National Injustice: Chevrontexaco And Indigenous Huaorani And Kichwa In The Amazon Rainforest In Ecuador, Judith Kimerling

Publications and Research

No abstract provided.

Rethinking Decisionmaking In International Environmental Law: A Process - Oriented Inquiry Into Sustainable Development, Rebecca Bratspies

Publications and Research

No abstract provided.

Some Thoughts On The American Approach To Regulating Genetically Modified Organisms, Rebecca Bratspies

Publications and Research

No abstract provided.

Lessons From Equal Opportunity Harasser Doctrine: Challenging Sex-Specific Appearance And Dress Codes, Deborah Zalesne

Publications and Research

No abstract provided.