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Prudence And Controversy: The New York Public Library Responds To Post-War Anticommunist Pressures, Stephen Francoeur Sep 2011

Prudence And Controversy: The New York Public Library Responds To Post-War Anticommunist Pressures, Stephen Francoeur

Publications and Research

As the New York Public Library entered the post-war era in the late 1940s, its operations fell under the zealous scrutiny of self-styled ‘redhunters’ intent upon rooting out library materials and staffers deemed un-American and politically subversive. The high point of attacks upon the New York Public Library came during the years 1947-1954, a period that witnessed the Soviet atomic bomb, the Berlin airlift, and the Korean War. This article charts the narrow and carefully wrought trail blazed by the library’s leadership during that period. Through a reading of materials in the library archives, we see how political pressures were …


In The Name Of Citizenship: The Writing Classroom And The Promise Of Citizenship, Amy J. Wan Sep 2011

In The Name Of Citizenship: The Writing Classroom And The Promise Of Citizenship, Amy J. Wan

Publications and Research

No abstract provided.


Pirates And Librarians: Big Media, Technology And The Role Of Liberal Education, D. Aram Donabedian, John Carey Sep 2011

Pirates And Librarians: Big Media, Technology And The Role Of Liberal Education, D. Aram Donabedian, John Carey

Publications and Research

The widespread appearance of computers in libraries during the early 1990s elicited a debate among those who welcomed new technologies and those who perceived such changes as a threat to the traditional role of academic libraries and the values of liberal education. At the same time, increasing consolidation of major media channels—including sources of scholarly communication—has allowed a small number of corporations to control distribution and access to the materials libraries offer, through tools such as licensing fees, copyright restrictions, and digital rights management. In response to these barriers, librarians and educators have embraced open access publishing and Creative Commons …


Genomic And Karyotypic Variation In Drosophila Parasitoids (Hymenoptera, Cynipoidea, Figitidae), Vladimir E. Gokhman, J. Spencer Johnston, Chiyedza Small, Roma Rajwani, Shawn J. Hanrahan, Shubha Govind Aug 2011

Genomic And Karyotypic Variation In Drosophila Parasitoids (Hymenoptera, Cynipoidea, Figitidae), Vladimir E. Gokhman, J. Spencer Johnston, Chiyedza Small, Roma Rajwani, Shawn J. Hanrahan, Shubha Govind

Publications and Research

Abstract Drosophila melanogaster Meigen, 1830 has served as a model insect for over a century. Sequencing of the 11 additional Drosophila Fallen, 1823 species marks substantial progress in comparative genomics of this genus. By comparison, practically nothing is known about the genome size or genome sequences of parasitic wasps of Drosophila. Here, we present the first comparative analysis of genome size and karyotype structures of Drosophila parasitoids of the Leptopilina Förster, 1869 and Ganaspis Förster, 1869 species. The gametic genome size of Ganaspis xanthopoda (Ashmead, 1896) is larger than those of the three Leptopilina species studied. The genome sizes of …


Media Services In North American Academic Libraries, Michael J. Miller Aug 2011

Media Services In North American Academic Libraries, Michael J. Miller

Publications and Research

Ongoing evolution of the Benjamin S. Rosenthal (BRL), Multimedia Commons was initially informed by a report from an internal task force and also by a four-year-long study of North American academic library media services units. A travel grant allowed for visits to the media service units of Barnard College, College of Staten Island - City University of New York, Columbia University, Georgetown University, New York University, University of Maryland - College Park, University of Massachusetts – Amherst, University of North Carolina - Chapel Hill, University of Texas – Austin. Visits have subsequently been made to the University of Washington – …


Group 11 Metal Compounds With Tripodal Bis(Imidazole) Thioether Ligands. Applications As Catalysts In The Oxidation Of Alkenes And As Antimicrobial Agents, Fangwei Liu, Reema Anis, Eunmi Hwang, Rafael Ovalle, Armando Varela-Ramírez, Renato J. Aguilera, María Contel Aug 2011

Group 11 Metal Compounds With Tripodal Bis(Imidazole) Thioether Ligands. Applications As Catalysts In The Oxidation Of Alkenes And As Antimicrobial Agents, Fangwei Liu, Reema Anis, Eunmi Hwang, Rafael Ovalle, Armando Varela-Ramírez, Renato J. Aguilera, María Contel

Publications and Research

New group 11 metal complexes have been prepared using the previously described tripodal bis(imidazole) thioether ligand (N-methyl-4,5-diphenyl-2- imidazolyl)2C(OMe)C(CH3)2S(tert-Bu) ({BITOMe,StBu}, 2). The pincer ligand offers a N2S donor atom set that can be used to coordinate the group 11 metals in different oxidation states [AuI , AuIII, AgI , CuI and CuII]. Thus the new compounds [Au{BITOMe,StBu}Cl][AuCl4]2 (3), [Au{BITOMe,StBu}Cl] (4), [Ag{BITOMe,StBu}X] (X = OSO2CF3 - 5, PF6 - 6) and [Cu{BITOMe,StBu}Cl2] (7) have been synthesized from reaction of 2 with the appropriate metal precursors, and characterized in solution. While attempting characterization in the solid state of 3, single crystals of the …


Factors Influencing Lysis Time Stochasticity In Bacteriophage L, John J. Dennehy, Ing-Nang Wang Aug 2011

Factors Influencing Lysis Time Stochasticity In Bacteriophage L, John J. Dennehy, Ing-Nang Wang

Publications and Research

Background Despite identical genotypes and seemingly uniform environments, stochastic gene expression and other dynamic intracellular processes can produce considerable phenotypic diversity within clonal microbes. One trait that provides a good model to explore the molecular basis of stochastic variation is the timing of host lysis by bacteriophage (phage).

Results Individual lysis events of thermally-inducible l lysogens were observed using a temperature-controlled perfusion chamber mounted on an inverted microscope. Both mean lysis time (MLT) and its associated standard deviation (SD) were estimated. Using the SD as a measure of lysis time stochasticity, we showed that lysogenic cells in controlled environments varied …


Effectiveness Of A Combined Home Visiting And Group Intervention For Low Income African American Mothers: The Pride In Parenting Program, Kathy S. Katz, Marian H. Jarrett, Ayman El-Mohandes, Susan Schneider, Doris Mcneely-Johnson, Michele Kiely Jul 2011

Effectiveness Of A Combined Home Visiting And Group Intervention For Low Income African American Mothers: The Pride In Parenting Program, Kathy S. Katz, Marian H. Jarrett, Ayman El-Mohandes, Susan Schneider, Doris Mcneely-Johnson, Michele Kiely

Publications and Research

Intervention strategies are needed to improve maternal and infant outcomes in minority populations living in poverty. Home visiting by nurses has improved outcomes for mothers and young children, but use of professional staff makes these programs expensive. Pride in Parenting was a randomized controlled trial of paraprofessional home visitation to provide health and developmental intervention for high-risk African American mothers in Washington, DC. This study proposed to test whether paraprofessional visitors drawn from the community could effectively influence health and mothers; parenting behaviors and attitudes. African American mothers with inadequate prenatal care were recruited at delivery and randomized to intervention …


Changes In Energy Content Of Lunchtime Purchases From Fast Food Restaurants After Introduction Of Calorie Labelling: Cross Sectional Customer Surveys, Tamara Dumanovsky, Christina Y. Huang, Cathy A. Nonas, Thomas D. Matte, Mary T. Bassett, Lynn D. Silver Jul 2011

Changes In Energy Content Of Lunchtime Purchases From Fast Food Restaurants After Introduction Of Calorie Labelling: Cross Sectional Customer Surveys, Tamara Dumanovsky, Christina Y. Huang, Cathy A. Nonas, Thomas D. Matte, Mary T. Bassett, Lynn D. Silver

Publications and Research

Objective To assess the impact of fast food restaurants adding calorie labelling to menu items on the energy content of individual purchases. Design Cross sectional surveys in spring 2007 and spring 2009 (one year before and nine months after full implementation of regulation requiring chain restaurants’ menus to contain details of the energy content of all menu items). Setting 168 randomly selected locations of the top 11 fast food chains in New York City during lunchtime hours. Participants 7309 adult customers interviewed in 2007 and 8489 in 2009. Main outcome measures Energy content of individual purchases, based on customers’ register …


The Role Of Information Technology In Fulfilling The Promise Of Corporate Social Responsibility, David Salb, Hershey H. Friedman, Linda Weiser Friedman Jul 2011

The Role Of Information Technology In Fulfilling The Promise Of Corporate Social Responsibility, David Salb, Hershey H. Friedman, Linda Weiser Friedman

Publications and Research

Young people today want to work at a meaningful job and make a difference in the world. Information technology (IT) is an effective enabler for all sorts of business strategies, so it comes as no surprise that IT is useful for implementing a firm's corporate social responsibility (CSR) initiative as well. The computer professional of the twenty-first century has the tools, training, and talent to be involved in strategic decision making that will have a lasting impact on society. Computer technology can be used to increase profits; but it can also be used to enhance the workplace, create adaptations for …


Fathers' Time Investments In Children: Do Sons Get More?, Kristin Mammen Jul 2011

Fathers' Time Investments In Children: Do Sons Get More?, Kristin Mammen

Publications and Research

Evidence suggests that, from birth, fathers treat sons differently than daughters in the U.S., as well as in developing countries. Fathers' time investments in children are one channel through which differential treatment by gender may affect children's outcomes. This paper uses data from the 2003 American Time Use Survey to explore three questions about paternal time in married two-parent families: Does the gender composition of his children affect the amount of time a father spends with them? If so, does the gender of the individual child have an additional effect? And is a girl advantaged or disadvantaged by the presence …


Transcription And Translation Of Human F11r Gene Are Required For An Initial Step Of Atherogenesis Induced By Inflammatory Cytokines, Bani M. Azari, Jonathan D. Marmur, Moro O. Salifu, Yigal H. Ehrlich, Elizabeth Kornecki, Anna Babinska Jun 2011

Transcription And Translation Of Human F11r Gene Are Required For An Initial Step Of Atherogenesis Induced By Inflammatory Cytokines, Bani M. Azari, Jonathan D. Marmur, Moro O. Salifu, Yigal H. Ehrlich, Elizabeth Kornecki, Anna Babinska

Publications and Research

Background - The F11 Receptor (F11R; aka JAM-A, JAM-1) is a cell adhesion protein present constitutively on the membrane surface of circulating platelets and within tight junctions of endothelial cells (ECs). Previous reports demonstrated that exposure of ECs to pro-inflammatory cytokines causes insertion of F11R molecules into the luminal surface of ECs, ensuing with homologous interactions between F11R molecules of platelets and ECs, and a resultant adhesion of platelets to the inflamed ECs. The main new finding of the present report is that the first step in this chain of events is the de-novo transcription and translation of F11R molecules, …


Weight-Related Behavior Among Adolescents: The Role Of Peer Effects, Mir M. Ali, Aliaksandr Amialchuk, Frank W. Heiland Jun 2011

Weight-Related Behavior Among Adolescents: The Role Of Peer Effects, Mir M. Ali, Aliaksandr Amialchuk, Frank W. Heiland

Publications and Research

Purpose To investigate whether social interactions in friendship networks influence the following weight-related behaviors of adolescents: exercising regularly, playing an active sport, hours of TV/Video viewing, sleeping six or fewer hours, eating breakfast on weekdays, frequency of eating at fast food restaurants, eating five servings of fruits/vegetables daily, and consuming calorie-dense snacks.

Method Data from a nationally representative sample of adolescents are used to examine the association between peer and individual weight-related behaviors. Evidence from multivariate regression analysis controlling for an extensive list of individual- and family-level factors as well as school-level unobserved heterogeneity is obtained. Results We find a …


“What” And “Where” In Auditory Sensory Processing: A High-Density Electrical Mapping Study Of Distinct Neural Processes Underlying Sound Object Recognition And Sound Localization, Victoria M. Leavitt, Sophie Molholm, Manuel Gomez-Ramirez, John J. Foxe Jun 2011

“What” And “Where” In Auditory Sensory Processing: A High-Density Electrical Mapping Study Of Distinct Neural Processes Underlying Sound Object Recognition And Sound Localization, Victoria M. Leavitt, Sophie Molholm, Manuel Gomez-Ramirez, John J. Foxe

Publications and Research

Functionally distinct dorsal and ventral auditory pathways for sound localization (WHERE) and sound object recognition (WHAT) have been described in non-human primates. A handful of studies have explored differential processing within these streams in humans, with highly inconsistent findings. Stimuli employed have included simple tones, noise bursts, and speech sounds, with simulated left–right spatial manipulations, and in some cases participants were not required to actively discriminate the stimuli. Our contention is that these paradigms were not well suited to dissociating processing within the two streams. Our aim here was to determine how early in processing we …


Smoking Cessation And Relapse Among Pregnant African-American Smokers In Washington, Dc, Ayman El-Mohandes, M. Nabil El-Khorazaty, Michele Kieley, Marie G. Gantz Jun 2011

Smoking Cessation And Relapse Among Pregnant African-American Smokers In Washington, Dc, Ayman El-Mohandes, M. Nabil El-Khorazaty, Michele Kieley, Marie G. Gantz

Publications and Research

Smoking is the single most preventable cause of perinatal morbidity. This study examines smoking behaviors during pregnancy in a high risk population of African Americans. The study also examines risk factors associated with smoking behaviors and cessation in response to a cognitive behavioral therapy (CBT) intervention. This study is a secondary analysis of data from a randomized controlled trial addressing multiple risks during pregnancy. Five hundred African-American Washington, DC residents who reported smoking in the 6 months preceding pregnancy were randomized to a CBT intervention. Psycho-social and behavioral data were collected. Self-reported smoking and salivary cotinine levels were measured prenatally …


Automated Auction Mechanism Design With Competing Markets, Jinzhong Niu Jun 2011

Automated Auction Mechanism Design With Competing Markets, Jinzhong Niu

Publications and Research

Resource allocation is a major issue in multiple areas of computer science. Despite the wide range of resource types across these areas, for example real commodities in e-commerce and computing resources in distributed computing, auctions are commonly used in solving the optimization problems involved in these areas, since well designed auctions achieve desirable economic outcomes.

Auctions are markets with strict regulations governing the information available to traders in the market and the possible actions they can take. Auction mechanism design aims to manipulate the rules of an auction in order to achieve specific goals. Economists traditionally use mathematical methods, mainly …


Fluid Shear Stress Regulates The Invasive Potential Of Glioma Cells Via Modulation Of Migratory Activity And Matrix Metalloproteinase Expression, Henry Qazi, Zhong-Dong Shi, John M. Tarbell May 2011

Fluid Shear Stress Regulates The Invasive Potential Of Glioma Cells Via Modulation Of Migratory Activity And Matrix Metalloproteinase Expression, Henry Qazi, Zhong-Dong Shi, John M. Tarbell

Publications and Research

Background Glioma cells are exposed to elevated interstitial fluid flow during the onset of angiogenesis, at the tumor periphery while invading normal parenchyma, within white matter tracts, and during vascular normalization therapy. Glioma cell lines that have been exposed to fluid flow forces in vivo have much lower invasive potentials than in vitro cell motility assays without flow would indicate. Methodology/Principal Findings A 3D Modified Boyden chamber (Darcy flow through collagen/cell suspension) model was designed to mimic the fluid dynamic microenvironment to study the effects of fluid shear stress on the migratory activity of glioma cells. Novel methods for gel …


A Transcriptional Analysis Of Carotenoid, Chlorophyll And Plastidial Isoprenoid Biosynthesis Genes During Development And Osmotic Stress Responses In Arabidopsis Thaliana, Stuart Meier, Oren Tzfadia, Ratnakar Vallabhaneni, Chris Gehring, Eleanore T. Wurtzel May 2011

A Transcriptional Analysis Of Carotenoid, Chlorophyll And Plastidial Isoprenoid Biosynthesis Genes During Development And Osmotic Stress Responses In Arabidopsis Thaliana, Stuart Meier, Oren Tzfadia, Ratnakar Vallabhaneni, Chris Gehring, Eleanore T. Wurtzel

Publications and Research

Background The carotenoids are pure isoprenoids that are essential components of the photosynthetic apparatus and are coordinately synthesized with chlorophylls in chloroplasts. However, little is known about the mechanisms that regulate carotenoid biosynthesis or the mechanisms that coordinate this synthesis with that of chlorophylls and other plastidial synthesized isoprenoid-derived compounds, including quinones, gibberellic acid and abscisic acid. Here, a comprehensive transcriptional analysis of individual carotenoid and isoprenoid-related biosynthesis pathway genes was performed in order to elucidate the role of transcriptional regulation in the coordinated synthesis of these compounds and to identify regulatory components that may mediate this process in Arabidopsis …


Changes In The Regulatory Environment And There Effects On Audits And Auditors, Margaret Ann Wood May 2011

Changes In The Regulatory Environment And There Effects On Audits And Auditors, Margaret Ann Wood

Publications and Research

No abstract provided.


Cultures In The Making: An Examination Of The Ethical And Methodological Implications Of Collaborative Research, Chrstina Siry, Carolyne Ali-Khan, Mark Zuss May 2011

Cultures In The Making: An Examination Of The Ethical And Methodological Implications Of Collaborative Research, Chrstina Siry, Carolyne Ali-Khan, Mark Zuss

Publications and Research

This paper explores ethical and methodological implications of collaborative research, and we discuss our examination of ways to work towards participatory, ethical relationships in research. Our core concerns pertain to the experiential, lived and qualitative relations within emergent research communities. Questions that have guided us include: What does "we" mean in research practice? How do we become a community of researchers? What forms of relations are shaped in the continuous process of inquiry? Whose interests are served? How can a community of researchers and their participants, formed and sustained by reciprocal, ethical relations, of trust, shared knowledges, curiosity and friendship, …


Understanding Depressive Symptoms Among High-Risk Pregnant African American Women, Kristine M. Molina, Michele Kiely May 2011

Understanding Depressive Symptoms Among High-Risk Pregnant African American Women, Kristine M. Molina, Michele Kiely

Publications and Research

Much has been written about depression and its risk factors, particularly among women. However, a growing body of literature on prenatal depression has begun to emerge, given that depression during pregnancy presents a major public health concern, since it is found to be a common experience among childbearing aged women (Gaynes et al., 2005; Marcus, 2009). In fact, the onset of depressive symptoms is most likely to occur between 20 to 40 years of age, the range when most women become pregnant (Marcus & Heringhausen, 2009). Further, prior studies have found rates of depression among pregnant women to range from …


Transplantation Of Adult Mouse Ips Cell-Derived Photoreceptor Precursors Restores Retinal Structure And Function In Degenerative Mice, Budd A. Tucker, In-Hyun Park, Sara D. Qi, Henry J. Klassen, Caihui Jiang, Jing Yao, Stephen Redenti, George Q. Daley, Michael J. Young Apr 2011

Transplantation Of Adult Mouse Ips Cell-Derived Photoreceptor Precursors Restores Retinal Structure And Function In Degenerative Mice, Budd A. Tucker, In-Hyun Park, Sara D. Qi, Henry J. Klassen, Caihui Jiang, Jing Yao, Stephen Redenti, George Q. Daley, Michael J. Young

Publications and Research

This study was designed to determine whether adult mouse induced pluripotent stem cells (iPSCs), could be used to produce retinal precursors and subsequently photoreceptor cells for retinal transplantation to restore retinal function in degenerative hosts. iPSCs were generated using adult dsRed mouse dermal fibroblasts via retroviral induction of the transcription factors Oct4, Sox2, KLF4 and c-Myc. As with normal mouse ES cells, adult dsRed iPSCs expressed the pluripotency genes SSEA1, Oct4, Sox2, KLF4, c-Myc and Nanog. Following transplantation into the eye of immunecompromised retinal degenerative mice these cells proceeded to form teratomas containing tissue comprising all three germ layers. At …


Reflections On Juvenile Justice Reform In New York. Keynote Address Delivered At The Diane Abbey Law Center For Children And Families Conference In New York, Ny Law School., Jeremy Travis Apr 2011

Reflections On Juvenile Justice Reform In New York. Keynote Address Delivered At The Diane Abbey Law Center For Children And Families Conference In New York, Ny Law School., Jeremy Travis

Publications and Research

No abstract provided.


The Association Of Tree Pollen Concentration Peaks And Allergy Medication Sales In New York City: 2003–2008, Perry E. Sheffield, Kate R. Weinberger, Kazuhiko Ito, Thomas D. Matte, Robert W. Mathes, Guy S. Robinson, Patrick L. Kinney Apr 2011

The Association Of Tree Pollen Concentration Peaks And Allergy Medication Sales In New York City: 2003–2008, Perry E. Sheffield, Kate R. Weinberger, Kazuhiko Ito, Thomas D. Matte, Robert W. Mathes, Guy S. Robinson, Patrick L. Kinney

Publications and Research

The impact of pollen exposure on population allergic illness is poorly characterized. We explore the association of tree pollen and over-the-counter daily allergy medication sales in the New York City metropolitan area. Dates of peak tree pollen (maple, oak, and birch) concentrations were identified from 2003 to 2008. Daily allergy medication sales reported to the city health department were analyzed as a function of the same-day and lagged tree pollen peak indicators, adjusting for season, year, temperature, and day of week. Significant associations were found between tree pollen peaks and allergy medication sales, with the strongest association at 2-day lag …


Not So Fun City, William A. Casari Apr 2011

Not So Fun City, William A. Casari

Publications and Research

This article posits that the destruction of the South Bronx during the 1960s and 1970s was municipally approved across several mayoral administrations, most notably the term of John Lindsay who coined the catch phrase “Fun City,” referring to New York City on the first day of a transit strike. The author outlines several books and articles that detail the destruction caused by fires and city planning decisions that adversely affected White ethnic and neighborhoods of color in the South Bronx. Media portrayals during this period were used to stereotype certain areas of the Bronx which in turn allowed city planners …


Re-Engaging/Engaging Part-Time Librarians, Wilma L. Jones Apr 2011

Re-Engaging/Engaging Part-Time Librarians, Wilma L. Jones

Publications and Research

In order to maintain high quality service and strengthen retention of part-time librarians, this article addresses ways of engaging new part-time librarians and re-engaging long-time part-time librarians. Part-time librarians are hired primarily to work hours outside the traditional work week or as temporary replacements for unanticipated vacancies, yet they end up providing a third of the service, solely, during evenings and weekends in most small to mid-sized academic institutions. Most are brought in with very little training or orientation to the institution and are expected to hit the ground running while maintaining quality service. This article provides managers with suggestions …


Molar Macrowear Reveals Neanderthal Eco-Geographic Dietary Variation, Luca Fiorenza, Stefano Benazzi, Jeremy Tausch, Ottmar Kullmer, Timothy G. Bromage, Friedemann Schrenk Mar 2011

Molar Macrowear Reveals Neanderthal Eco-Geographic Dietary Variation, Luca Fiorenza, Stefano Benazzi, Jeremy Tausch, Ottmar Kullmer, Timothy G. Bromage, Friedemann Schrenk

Publications and Research

Neanderthal diets are reported to be based mainly on the consumption of large and medium sized herbivores, while the exploitation of other food types including plants has also been demonstrated. Though some studies conclude that early Homo sapiens were active hunters, the analyses of faunal assemblages, stone tool technologies and stable isotopic studies indicate that they exploited broader dietary resources than Neanderthals. Whereas previous studies assume taxon-specific dietary specializations, we suggest here that the diet of both Neanderthals and early Homo sapiens is determined by ecological conditions. We analyzed molar wear patterns using occlusal fingerprint analysis derived from optical 3D …


Electromagnetic Wave Propagation In Two-Dimensional Photonic Crystals, Oleg L. Berman, Vladimir S. Boyko, Roman Ya. Kezerashvili, Yurii E. Lozovik Mar 2011

Electromagnetic Wave Propagation In Two-Dimensional Photonic Crystals, Oleg L. Berman, Vladimir S. Boyko, Roman Ya. Kezerashvili, Yurii E. Lozovik

Publications and Research

No abstract provided.


Clean Image Synthesis And Target Numerical Marching For Optical Imaging With Backscattering Light, Min Xu, Yang Pu, Wubao Wang Mar 2011

Clean Image Synthesis And Target Numerical Marching For Optical Imaging With Backscattering Light, Min Xu, Yang Pu, Wubao Wang

Publications and Research

Scanning backscattering imaging and independent component analysis (ICA) are used to probe targets hidden in the subsurface of a turbid medium. A new correction procedure is proposed and used to synthesize a “clean” image of a homogeneous host medium numerically from a set of raster-scanned “dirty” backscattering images of the medium with embedded targets. The independent intensity distributions on the surface of the medium corresponding to individual targets are then unmixed using ICA of the difference between the set of dirty images and the clean image. The target positions are localized by a novel analytical method, which marches the target …


Bmp-12 Treatment Of Adult Mesenchymal Stem Cells In Vitro Augments Tendon-Like Tissue Formation And Defect Repair In Vivo, Jonathan Y. Lee, Zuping Zhou, Peter J. Taub, Melissa Ramcharan, Yonghui Li, Takintope Akinbiyi, Edward R. Maharam, Daniel J. Leong, Damien M. Laudier, Takuya Ruike, Phillip J. Torina, Mone Zaidi, Robert Majeska, Mitchell B. Schaffler, Evan L. Flatow, Hui B. Sun Mar 2011

Bmp-12 Treatment Of Adult Mesenchymal Stem Cells In Vitro Augments Tendon-Like Tissue Formation And Defect Repair In Vivo, Jonathan Y. Lee, Zuping Zhou, Peter J. Taub, Melissa Ramcharan, Yonghui Li, Takintope Akinbiyi, Edward R. Maharam, Daniel J. Leong, Damien M. Laudier, Takuya Ruike, Phillip J. Torina, Mone Zaidi, Robert Majeska, Mitchell B. Schaffler, Evan L. Flatow, Hui B. Sun

Publications and Research

We characterized the differentiation of rat bone marrow-derived mesenchymal stem cells (BM-MSCs) into tenocyte-like cells in response to bone morphogenetic protein-12 (BMP-12). BM-MSCs were prepared from Sprague-Dawley rats and cultured as monolayers. Recombinant BMP-12 treatment (10 ng/ml) of BM-MSCs for 12 hours in vitro markedly increased expression of the tenocyte lineage markers scleraxis (Scx) and tenomodulin (Tnmd) over 14 days. Treatment with BMP-12 for a further 12-hour period had no additional effect. Colony formation assays revealed that ∼80% of treated cells and their progeny were Scx- and Tnmd-positive. BM-MSCs seeded in collagen scaffolds and similarly treated with a single dose …