Digital Commons Network^™

Egg Eviction Imposes A Recoverable Cost Of Virulence In Chicks Of A Brood Parasite, Michael G. Anderson, Csaba Moskát, Miklós Bán, Tomáš Grim, Phillip Cassey, Mark E. Hauber

Publications and Research

Background Chicks of virulent brood parasitic birds eliminate their nestmates and avoid costly competition for foster parental care. Yet, efforts to evict nest contents by the blind and naked common cuckoo Cuculus canorus hatchling are counterintuitive as both adult parasites and large older cuckoo chicks appear to be better suited to tossing the eggs and young of the foster parents. Methodology/Principal Findings Here we show experimentally that egg tossing imposed a recoverable growth cost of mass gain in common cuckoo chicks during the nestling period in nests of great reed warbler Acrocephalus arundinaceus hosts. Growth rates of skeletal traits and …

Contexts Of Exit In The Migration Of Russian Speakers From The Baltic Countries To Ireland, Sofya Aptekar

Publications and Research

Recently, Ireland has become a major destination for migrants from Estonia, Latvia, and Lithuania. Many of these migrants are members of Russian-speaking minorities leaving a context of restrictive citizenship and language laws and varying degrees of ethnic tension. This paper draws on interviews collected in Ireland to examine the role played by the contexts of exit in decisions to migrate among Russian-speaking minorities from the Baltics. The results suggest that Russian speakers from Estonia migrate because of their experiences as minorities, while those from Latvia and Lithuania migrate to escape low wages and irregular employment. This is so despite equally …

Correlation Between An Email Based Board Review Program And American Board Of Pediatrics General Pediatrics Certifying Examination Scores, Erik E. Langenau, Joshua Fogel, Henry A. Schaeffer

Publications and Research

Objective To investigate the impact of a weekly email based board review course on individual resident performance on the American Board of Pediatrics (ABP) General Pediatrics Certifying Examination for pediatric residents and, specifically, residents with low ABP In-training Examination (ITE) scores. Methods Weekly board-type questions were emailed to all pediatric residents from 2004–2007. Responses to board-type questions were tracked, recorded, and correlated with ITE scores and ABP General Pediatrics Certifying Examination Scores. Results With regard to total number of questions answered, only total number of questions answered correctly had a significant positive correlation with standard board scores (n = 71, …

Correlating Molecular Phylogeny With Venom Apparatus Occurrence In Panamic Auger Snails (Terebridae), Mandë Holford, Nicolas Puillandre, Maria Vittoria Modica, Maren Watkins, Rachel Collin, Eldredge Bermingham, Baldomero M. Olivera

Publications and Research

Central to the discovery of neuroactive compounds produced by predatory marine snails of the superfamily Conoidea (cone snails, terebrids, and turrids) is identifying those species with a venom apparatus. Previous analyses of western Pacific terebrid specimens has shown that some Terebridae groups have secondarily lost their venom apparatus. In order to efficiently characterize terebrid toxins, it is essential to devise a key for identifying which species have a venom apparatus. The findings presented here integrate molecular phylogeny and the evolution of character traits to infer the presence or absence of the venom apparatus in the Terebridae. Using a combined dataset …

Pointcut Rejuvenation: Recovering Pointcut Expressions In Evolving Aspect-Oriented Software, Raffi Khatchadourian, Phil Greenwood, Awais Rashid, Guoqing Harry Xu

Publications and Research

Pointcut fragility is a well-documented problem in Aspect-Oriented Programming; changes to the base-code can lead to join points incorrectly falling in or out of the scope of pointcuts. We present an automated approach that limits fragility problems by providing mechanical assistance in pointcut maintenance. The approach is based on harnessing arbitrarily deep structural commonalities between program elements corresponding to join points selected by a pointcut. The extracted patterns are then applied to later versions to offer suggestions of new join points that may require inclusion. We demonstrate the usefulness of our technique by rejuvenating pointcuts in multiple versions of several …

Trance, Edgar Nkosi White

Publications and Research

No abstract provided.

Manipulating Neuronal Circuits With Endogenous And Recombinant Cell-Surface Tethered Modulators, Mande Holford, Sebastian Auer, Martin Laqua, Ines Ibanez-Tallon

Publications and Research

Neuronal circuits depend on the precise regulation of cell-surface receptors and ion channels. An ongoing challenge in neuroscience research is deciphering the functional contribution of specifi c receptors and ion channels using engineered modulators. A novel strategy, termed “tethered toxins”, was recently developed to characterize neuronal circuits using the evolutionary derived selectivity of venom peptide toxins and endogenous peptide ligands, such as lynx1 prototoxins. Herein, the discovery and engineering of cell-surface tethered peptides is reviewed, with particular attention given to their cell-autonomy, modular composition, and genetic targeting in different model organisms. The relative ease with which tethered peptides can be …

Mercury Exposure, Nutritional Deficiencies And Metabolic Disruptions May Affect Learning In Children, Renee Dufault, Roseanne Schnoll, Walter J. Lukiw, Blaise Leblanc, Charles Cornett, Lyn Patrick, David Wallinga, Steven G. Gilbert, Raquel Crider

Publications and Research

Among dietary factors, learning and behavior are influenced not only by nutrients, but also by exposure to toxic food contaminants such as mercury that can disrupt metabolic processes and alter neuronal plasticity. Neurons lacking in plasticity are a factor in neurodevelopmental disorders such as autism and mental retardation. Essential nutrients help maintain normal neuronal plasticity. Nutritional deficiencies, including deficiencies in the long chain polyunsaturated fatty acids eicosapentaenoic acid and docosahexaenoic acid, the amino acid methionine, and the trace minerals zinc and selenium, have been shown to influence neuronal function and produce defects in neuronal plasticity, as well as impact behavior …

The Accounting At A Tipping Point – Decline Or Renewal, Susan Haka

Publications and Research

No abstract provided.

Oldest Evidence Of Toolmaking Hominins In A Grassland-Dominated Ecosystem, Thomas W. Plummer, Peter W. Ditchfield, Laura C. Bishop, John D. Kingston, Joseph V. Ferraro, David R. Braun, Fritz Hertel, Richard Potts

Publications and Research

Background Major biological and cultural innovations in late Pliocene hominin evolution are frequently linked to the spread or fluctuating presence of C4 grass in African ecosystems. Whereas the deep sea record of global climatic change provides indirect evidence for an increase in C4 vegetation with a shift towards a cooler, drier and more variable global climatic regime beginning approximately 3 million years ago (Ma), evidence for grassland-dominated ecosystems in continental Africa and hominin activities within such ecosystems have been lacking. Methodology/Principal Findings We report stable isotopic analyses of pedogenic carbonates and ungulate enamel, as well as faunal data from ∼2.0 …

Development Of A Mechanical System For Osteon Isolation, S. Ranglin, D. Das, A. Mingo, O. Ukinamemen, Gaffar Gailani, S. Cowin, L. Cardoso

Publications and Research

Osteons are small semi cylindrical hard tissues that exist in long bones of humans and some animals. Their diameter is in the range of 250 – 300 micrometers approximately. They contain the osteocytes which plays a role in bone mechanotransduction. The boundary that surrounds the osteon from outside is called the cement line. In a summer research project supported by CUNY-LSAMP a group of four students and three professors worked full summer to develop a system that can isolate the osteons thus a mechanical testing could be performed (stress relaxation test) to determine their poroelastic properties. Two sets of osteons …

Organizational Life And Political Incorporation Of Two Asian Immigrant Groups: A Case Study, Sofya Aptekar

Publications and Research

Civil society is the foundation of a healthy democracy but its immigrant element has received little attention. This paper is a case study of immigrant organizations of highly skilled Asian Indians and Chinese immigrants in a suburban town of Edison, New Jersey. I find that civic participation of Asian Indian immigrants spills over into political incorporation while Chinese immigrant organizations remain margin- alized. I argue that local processes of racialization are central in explaining differences in political incorporation of immigrants. In the local context, the Chinese are seen as successful but conformist model minorities and Asian Indians as invaders and …

Cooperative Social Clusters Are Not Destroyed By Dispersal In A Ciliate, Nicolas Schtickzelle, Else J. Fjerdingstad, Alexis Chaine, Jean Clobert

Publications and Research

Background The evolution of social cooperation is favored by aggregative behavior to facilitate stable social structure and proximity among kin. High dispersal rates reduce group stability and kin cohesion, so it is generally assumed that there is a fundamental trade-off between cooperation and dispersal. However, empirical tests of this relationship are rare. We tested this assumption experimentally using ten genetically isolated strains of a ciliate, Tetrahymena thermophila. Results The propensity for social aggregation was greater in strains with reduced cell quality and lower growth performance. While we found a trade-off between costly aggregation and local dispersal in phenotypic analyses, aggregative …

Harnessing Gene Expression To Identify The Genetic Basis Of Drug Resistance, Bo-Juen Chen, Helen C. Causton, Denesy Mancenido, Noel L. Goddard, Ethan O. Perlstein, Dana Pe'er

Publications and Research

The advent of cost-effective genotyping and sequencing methods have recently made it possible to ask questions that address the genetic basis of phenotypic diversity and how natural variants interact with the environment. We developed Camelot (CAusal Modelling with Expression Linkage for cOmplex Traits), a statistical method that integrates genotype, gene expression and phenotype data to automatically build models that both predict complex quantitative phenotypes and identify genes that actively influence these traits. Camelot integrates genotype and gene expression data, both generated under a reference condition, to predict the response to entirely different conditions. We systematically applied our algorithm to data …

Activation Of Cyclin-Dependent Kinase 5 Is A Consequence Of Cell Death, Yixia Ye, Antonella Tinari, Walter Malorni, Zahra Zakeri

Publications and Research

Cyclin-dependent kinase 5 (Cdk5) is similar to other Cdks but is activated during cell differentiation and cell death rather than cell division. Since activation of Cdk5 has been reported in many situations leading to cell death, we attempted to determine if it was required for any form of cell death. We found that Cdk5 is activated during apoptotic deaths and that the activation can be detected even when the cells continue to secondary necrosis. This activation can occur in the absence of Bim, calpain, or neutral cathepsins. The kinase is typically activated by p25, derived from p35 by calpain-mediated cleavage, …

Poly-Paraphyly Of Hirudinidae: Many Lineages Of Medicinal Leeches, Anna J. Phillips, Mark E. Siddall

Publications and Research

Background: Medicinal leeches became infamous for their utility in bloodletting popularized in the 19th century, and have seen a recent resurgence in post-operative treatments for flap and replantation surgeries, and in terms of characterization of salivary anticoagulants. Notorious throughout the world, the quintessential leech family Hirudinidae has been taken for granted to be monophyletic, as has the non-bloodfeeding family Haemopidae.

Results: This study is the first to evaluate molecular evidence from hirudinid and haemopid leeches in a manner that encompasses the global scope of their taxonomic distributions. We evaluated the presumed monophyly of the Hirudinidae and assessed previous well-accepted classification …

Comparison Of Spatial Summation Properties Of Neurons In Macaque V1 And V2, S. Shushruth, Jennifer M. Ichida, Jonathan B. Levitt, Alessandra Angelucci

Publications and Research

In visual cortex, responses to stimulation of the receptive field (RF) are modulated by simultaneous stimulation of the RF surround. The mechanisms for surround modulation remain unidentified. We previously proposed that in the primary visual cortex (V1), near surround modulation is mediated by geniculocortical and horizontal connections and far surround modulation by interareal feedback connections. To understand spatial integration in the secondary visual cortex (V2) and its underlying circuitry, we have characterized spatial summation in different V2 layers and stripe compartments and compared it to that in V1. We used grating stimuli in circular and annular apertures of different sizes …

Cooperation Between A Coenzyme A-Independent Stand-Alone Initiation Module And An Iterative Type I Polyketide Synthase During Synthesis Of Mycobacterial Phenolic Glycolipids, Weiguo He, Clifford E. Soll, Sivagami Sundaram Chavadi, Guangtao Zhang, J. David Warren, Luis E. N. Quadri

Publications and Research

Several Mycobacterium tuberculosis strains, Mycobacterium leprae, and other mycobacterial pathogens produce a group of small-molecule virulence factors called phenolic glycolipids (PGLs). PGLs play key roles in pathogenicity and host−pathogen interaction. Thus, elucidation of the PGL biosynthetic pathway will not only expand our understanding of natural product biosynthesis, but may also illuminate routes to novel therapeutics to afford alternative lines of defense against mycobacterial infections. In this study, we report an investigation of the enzymatic requirements for the production of long-chain p-hydroxyphenylalkanoate intermediates of PGL biosynthesis. We demonstrate a functional cooperation between a coenzyme A-independent stand-alone didomain initiation module (FadD22) and …

Prediction Of Birth Weight By Cotinine Levels During Pregnancy In A Population Of African American Smokers, Ayman A.E. El-Mohandes, Michele Kiely, Marie G. Gantz, Susan M. Blake, M Nabil El-Khorazaty

Publications and Research

Objective—The goal was to investigate the association between maternal salivary cotinine levels (SCLs) and pregnancy outcome among African Americans smokers

Methods—In a randomized controlled trial conducted in 2001-2004 in Washington, D.C. 714 women (126 active smokers (18%)) were tested for SCLs at the time of recruitment and later in pregnancy. Sociodemographic health risks and pregnancy outcomes were recorded.

Results—Birth weights were significantly lower for infants born to mothers with baseline SCLs of ≥20 ng/ml compared to/ml (p=0.024), ≥50 ng/ml compared to/ml (p=0.002), ≥100 ng/ml compared to/ml (p=0.002), in bivariate analyses. In linear regression analyses adjusting for sociodemographic …

Contributing Factors To Pointcut Fragility, Phil Greenwood, Awais Rashid, Raffi Khatchadourian

Publications and Research

Pointcut fragility is a well-documented problem of Aspect-Oriented Programming with changes to the base-code causing join points to incorrectly fall in or out of scope. In order to combat this problem a tool was developed that provides mechanical assistance to pointcut maintenance. This tool relied on the deep structural commonalities between program elements to detect when pointcut fragility occurs. During the assessment of this tool a number of common practices were uncovered that were employed both in the aspect and base-code that contributed to or prevented pointcut fragility. This paper documents the practices uncovered and describes how they can affect …

Untangling The Relationship Between Libraries, Young Adults And Web 2.0: The Necessity Of A Critical Perspective, Lucia Cedeira Serantes

Publications and Research

Purpose: This paper addresses a gap in the analysis of the dynamic and challenging relationship between libraries, Web 2.0 and young adults, suggesting the relevance of a critical approach.

Methodology/Approach: This paper represents an exploratory literature review with the objective of identifying a possible gap in the way the LIS community is addressing the concept of Web 2.0.

Findings: Findings indicate that the research produced in other fields, such as Communication or Computer Science; the way young adults interrelate with new technologies; and the need for collaboration between practitioners and researchers justify and support the use of a critical perspective …

Ron’S Right Arm: Tactility, Visualization, And The Synesthesia Of Audio Engineering, Eliot Bates

Publications and Research

Most scholarship on audio engineering analyzes practices and practitioners in terms of musical and technical knowledges. The few references to sensory perception typically center on critical listening practices (“golden ears” engineers), audiophilia, and technologies of audition. However, particularly in light of computer-based workflows, the practice of audio engineering features carefully developed synesthesias of critical listening, visualization of digital audio, and tactile manipulations of interfaces, which can’t adequately be explained as cognitive processes or as conscious knowledge.

I draw on literature in the emerging field of sensory scholarship, in particular Brian Massumi’s theorization of synesthesia and affect and Charles Hirschkind’s analyses …

Extra, Extra, Read All About It!, Irvin Sam Schonfeld

Publications and Research

No abstract provided.

Identifying Publication Outlets In Occupational Health Psychology: An Opinion Survey, Maria Karanika-Murray, Irvin Sam Schonfeld, Leigh Schmitt

Publications and Research

No abstract provided.

A Randomised-Controlled Trial Of Two Educational Modes For Undergraduate Evidence-Based Medicine Learning In Asia, Janice M. Johnston, Mary Schooling, Gabriel M. Leung

Publications and Research

Background
As the overall evidence for the effectiveness of teaching of evidence based medicine (EBM) is not strong, and the impact of cultural and societal influences on teaching method is poorly understood, we undertook a randomised-controlled trial to test the effectiveness and learning satisfaction with two different EBM teaching methods (usual teaching vs. problem based learning (PBL)) for undergraduate medical students.

Methods
A mixed methods study that included a randomised-controlled crossover trial with two intervention arms (usual teaching and PBL) and a nested qualitative study with focus groups to explore student perceptions of learning and to assess the effectiveness and …

Optimizing Taq Polymerase Concentration For Improved Signal-To-Noise In The Broad Range Detection Of Low Abundance Bacteria, Rudolph Spangler, Noel L. Goddard, David S. Thaler

Publications and Research

Background PCR in principle can detect a single target molecule in a reaction mixture. Contaminating bacterial DNA in reagents creates a practical limit on the use of PCR to detect dilute bacterial DNA in environmental or public health samples. The most pernicious source of contamination is microbial DNA in DNA polymerase preparations. Importantly, all commercial Taq polymerase preparations inevitably contain contaminating microbial DNA. Removal of DNA from an enzyme preparation is problematical. Methodology/Principal Findings This report demonstrates that the background of contaminating DNA detected by quantitative PCR with broad host range primers can be decreased greater than 10-fold through the …

Xmc Mediates Xctr1-Independent Morphogenesis In Xenopus Laevis†, Tomomi Haremaki, Daniel C. Weinstein

Publications and Research

In the frog, Xenopus laevis, fibroblast growth factor (FGF) signaling is required for both mesoderm formation and the morphogenetic movements that drive the elongation of the notochord, a dorsal mesodermal derivative; the coordination of these distinct roles is mediated by the Xenopus Ctr1 (Xctr1) protein: maternal Xctr1 is required for mesodermal differentiation, while the subsequent loss of Xctr1 promotes morphogenesis. The signaling cascade activated by FGF in the presence of Ctr1 has been well characterized; however, the Xctr1-independent, FGF-responsive network remains poorly defined. We have identified Xenopus Marginal Coil (Xmc) as a gene whose expression is highly enriched following Xctr1 …

Three-Dimensional Structure Of The Enveloped Bacteriophage Φ12: An Incomplete T = 13 Lattice Is Superposed On An Enclosed T = 1 Shell, Hui Wei, R. Holland Cheng, John Berriman, William J. Rice, David L. Stokes, A. Katz, David Gene Morgan, Paul Gottlieb

Publications and Research

Background Bacteriophage φ12 is a member of the Cystoviridae, a unique group of lipid containing membrane enveloped bacteriophages that infect the bacterial plant pathogen Pseudomonas syringae pv. phaseolicola. The genomes of the virus species contain three double-stranded (dsRNA) segments, and the virus capsid itself is organized in multiple protein shells. The segmented dsRNA genome, the multi-layered arrangement of the capsid and the overall viral replication scheme make the Cystoviridae similar to the Reoviridae. Methodology/Principal Findings We present structural studies of cystovirus φ12 obtained using cryo-electron microscopy and image processing techniques. We have collected images of isolated φ12 virions and generated …

The Evaluation Of Use Of Electronic Resources And Services In Academic Libraries: A Study Of E-Metrics And Related Methods For Measurement And Assessment, Kanu A. Nagra

Publications and Research

This paper presents a review of literature for the evaluation of use of electronic resources and services in Academic libraries. E-metrics and similar methods for evaluation are discussed in detail. The paper highlights how some libraries approached e-metrics to start useful evaluations and dealt with technicalities to achieve better decision making for e-collection, services and infrastructure for their library users. The evaluation of use of e-resources and services is discussed in five parts explaining application of e-metrics, different methods to capture usage data in different settings, types of data requirement by libraries, complexities and technicalities involved in measurement of usage …

Situation Awareness Requirements Analysis For Emergency Management Planning, Norman E. Groner

Publications and Research

No abstract provided.