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In Polycistronic Qβ Rna, Single-Strandedness At One Ribosome Binding Site Directly Affects Translational Initiations At A Distal Upstream Cistron, Lalitha Jayant, Christine Priano, Donald R. Mills

Publications and Research

In Qβ RNA, sequestering the coat gene ribosome binding site in a putatively strong hairpin stem structure eliminated synthesis of coat protein and activated protein synthesis from the much weaker maturation gene initiation site, located 1300 nucleotides upstream. As the stability of a hairpin stem comprising the coat gene Shine–Dalgarno site was incrementally increased, there was a corresponding increase in translation of maturation protein. The effect of the downstream coat gene ribosome binding sequence on maturation gene expression appeared to have occurred only in cis and did not require an AUG start codon or initiation of coat protein synthesis. In …

Ectopic T Cell Receptor-Α Locus Control Region Activity In B Cells Is Suppressed By Direct Linkage To Two Flanking Genes At Once, Stefan Knirr, Janette Gomos-Klein, Blanca E. Andino, Faith Harrow, Karl F. Erhard, Damian Kovalovsky, Derek B. Sant'angelo, Benjamin D. Ortiz

Publications and Research

The molecular mechanisms regulating the activity of the TCRα gene are required for the production of the circulating T cell repertoire. Elements of the mouse TCRα locus control region (LCR) play a role in these processes. We previously reported that TCRα LCR DNA supports a gene expression pattern that mimics proper thymus-stage, TCRα gene-like developmental regulation. It also produces transcription of linked reporter genes in peripheral T cells. However, TCRα LCR-driven transgenes display ectopic transcription in B cells in multiple reporter gene systems. The reasons for this important deviation from the normal TCRα gene regulation pattern are unclear. In its …

A Richer And More Diverse Future For Cell Biology, Sandra L. Schmid, Molly Carnes, Ursula Goodenough, Nancy Hopkins, Phoebe Leboy, Sandra Masur, Virginia Valian

Publications and Research

No abstract provided.

The Origin Of Love: Love Songs As Represented In The Cinema Of Rock Operas, Benjamin Franz

Publications and Research

No abstract provided.

Sex Partnerships, Health, And Social Risks Of Young Men Leaving Jail: Analyzing Data From A Randomized Controlled Trial, Megha Ramaswamy, Nicholas Freudenberg

Publications and Research

Background Young men involved in the criminal justice system face disproportionately high rates of sexual risk behavior, drug, use, and violence. Little is known about how their involvement in sex partnerships might mitigate their unique health and social risks. This study explores whether sex partner experience protects against harmful sexual behaviors, drug problems, violence, and recidivism in 16-18-year-old Black and Latino men leaving a US jail. Methods Data were drawn from the Returning Educated African-American and Latino Men to Enriched Neighborhoods (REAL MEN) study conducted between 2003-2007, which tracked 552 adolescents during their time in a New York City jail …

Repair Of Mitomycin C Mono- And Interstrand Cross-Linked Dna Adducts By Uvrabc: A New Model, Mao-Wen Weng, Yi Zheng, Vijay P. Jasti, Elise Champeil, Maria Tomasz, Yinsheng Wang, Ashis K. Basu, Moon-Shong Tang

Publications and Research

Mitomycin C induces both MC-mono-dG and cross-linked dG-adducts in vivo. Interstrand cross-linked (ICL) dG-MC-dG-DNA adducts can prevent strand separation. In Escherichia coli cells, UvrABC repairs ICL lesions that cause DNA bending. The mechanisms and consequences of NER of ICL dG-MC-dG lesions that do not induce DNA bending remain unclear. Using DNA fragments containing a MC-mono-dG or an ICL dG-MC-dG adduct, we found (i) UvrABC incises only at the strand containing MC-mono-dG adducts; (ii) UvrABC makes three types of incisions on an ICL dG-MC-dG adduct: type 1, a single 5′ incision on 1 strand and a 3′ incision on the other; …

The Mycobacterium Tuberculosis Drugome And Its Polypharmacological Implications, Sarah L. Kinnings, Li Xie, Kingston H. Fung, Richard M. Jackson, Lei Xie, Phillip E. Bourne

Publications and Research

We report a computational approach that integrates structural bioinformatics, molecular modelling and systems biology to construct a drug-target network on a structural proteome-wide scale. The approach has been applied to the genome of Mycobacterium tuberculosis (M.tb), the causative agent of one of today’s most widely spread infectious diseases. The resulting drug-target interaction network for all structurally characterized approved drugs bound to putative M.tb receptors, we refer to as the ‘TB-drugome’. The TB-drugome reveals that approximately one-third of the drugs examined have the potential to be repositioned to treat tuberculosis and that many currently unexploited M.tb receptors may be chemically druggable …

Review Of The Website Founders Early Access, John A. Drobnicki

Publications and Research

Review of the website Founders early access.

Building Capacity For Public And Population Health Research In Africa: The Consortium For Advanced Research Training In Africa (Carta) Model, Alex C. Ezeh, Chimaraoke O. Izugbara, Caroline W. Kabiru, Sharon Fonn, Kathleen Kahn, Lenore Manderson, Akinyinka Omigbodun, Margaret Thorogood, Ashiwel S. Undieh

Publications and Research

Background: Globally, sub-Saharan Africa bears the greatest burden of disease. Strengthened research capacity to understand the social determinants of health among different African populations is key to addressing the drivers of poor health and developing interventions to improve health outcomes and health systems in the region. Yet, the continent clearly lacks centers of research excellence that can generate a strong evidence base to address the region’s socio-economic and health problems. Objective and program overview: We describe the recently launched Consortium for Advanced Research Training in Africa (CARTA), which brings together a network of nine academic and four research institutions from …

U.S. Multinationals In A World Of Uncertainty, Peter M. Robinson

Publications and Research

In this paper, Peter Robinson, president of the U.S. Council for International Business, provides his perspective on the challenges U.S. multinationals faced in the aftermath of the global financial crisis of 2007–2008, considered by many economists to have been the worst financial crisis since the Great Depression of the 1930s. As the American affiliate of the leading international business organizations, including the International Chamber of Commerce, the International Organization of Employers, and the Business and Industry Advisory Committee to the OECD, the USCIB provides business views to policy makers and regulatory authorities worldwide.

Review Of The Book Jewish Responses To Persecution, Vol. I: 1933-1938, John A. Drobnicki

Publications and Research

Review of the book Jewish responses to persecution, Vol. I: 1933-1938.

Accumulation, Excess, Childhood: Toward A Countertopography Of Risk And Waste, Cindi Katz

Publications and Research

This piece grows out of my on-going project, ‘Childhood as Spectacle’, and my enduring concern with social reproduction and what it does for and to Marxist and other critical political-economic analyses. After more than 30 years of Marxist-feminist interventions around these issues, symptomatic silences around social reproduction remain all too common in analyses of capitalism. Working through these issues and their occlusion, I offer what I hope is a useful and vibrant theoretical framework for examining geographies of children, youth, and families. Building this framework calls into play three overlapping issues; neoliberal capitalism in crisis and David Harvey’s notion of …

Ealles Englalandes Cyningc: Cnut's Territorial Kingship And Wulfstan's Paronomastic Play, Paul Gates

Publications and Research

The phrase ealles Englalandes cyningc appears for the first time in I–II Cnut, and represents a shift in the discourse of Anglo-Saxon kingship, changing it from king over a people to king over a territory, redefining the discourse of nationhood.

Overcoming The Model Minority Myth: Experiences Of Filipino American Graduate Students, Kevin L. Nadal, Stephanie T. Pituc, Marc P. Johnston, Theresa Esparrago

Publications and Research

Filipino Americans are one of the largest immigrant groups in the United States and the second largest Asian American/Pacific Islander ethnic group. However, there is little research focusing on the unique experiences of this group, particularly in higher education. This paper presents a qualitative exploration of the experiences of Filipino American graduate students utilizing consensual qualitative research methodology. Results were categorized into domains and themes, with an example of a domain being "deficiencies and lack of resources" and an example of a theme being "Filipino Americans as different from Asian Americans." Implications for higher education administrators and researchers are discussed.

Upper Cretaceous Peay Bentonites (North-Central Wyoming): Provenance And Tectonics Interpretation From Ash Composition, Nazrul I. Khandaker, Carl F. Vondra, Karl E. Seifert, Malek Shami, Rochelle Cardinale, Thakur Chaturgan

Publications and Research

The Peay bentonites belong to the basal Frontier Formation (Bighorn Basin, north-central Wyoming), primarily outcrop in the Bighorn Basin, rest on an extensively bioturbated sandstone unit, the Peay Sandstone, and are generally the thinner bentonitic unit. Beds of very light gray to greenish gray bentonite are also abundant in the lower Frontier units between Kaycee and Mayoworth (Powder River Basin) and are very rarely as much as 3 m thick. Bentonite occurs within the interstratified shale, sandstone, and siltstone sequences of the lower Frontier unit throughout much of the southwestern Powder River Basin. The purpose of this study is to …

Conserved Genes Act As Modifiers Of Invertebrate Smn Loss Of Function Defects, Maria Dimitriadi, James N. Sleigh, Amy Walker, Howard C. Chang, Anindya Sen, Geetika Kalloo, Jevede Harris, Tom Barsby, Melissa B. Walsh, John S. Satterlee, Chris Li, David Van Vactor, Spyros Artavanis-Tsakonas, Anne C. Hart

Publications and Research

Spinal Muscular Atrophy (SMA) is caused by diminished function of the Survival of Motor Neuron (SMN) protein, but the molecular pathways critical for SMA pathology remain elusive. We have used genetic approaches in invertebrate models to identify conserved SMN loss of function modifier genes. Drosophila melanogaster and Caenorhabditis elegans each have a single gene encoding a protein orthologous to human SMN; diminished function of these invertebrate genes causes lethality and neuromuscular defects. To find genes that modulate SMN function defects across species, two approaches were used. First, a genome-wide RNAi screen for C. elegans SMN modifier genes was undertaken, yielding …

Tensorial Reconstruction At The Integrand Level, G. Heinrich, Giovanni Ossola, T. Reiter, F. Tramontano

Publications and Research

We present a new approach to the reduction of one-loop amplitudes obtained by reconstructing the tensorial expression of the scattering amplitudes. The reconstruction is performed at the integrand level by means of a sampling in the integration momentum. There are several interesting applications of this novel method within existing techniques for the reduction of one-loop multi-leg amplitudes: to deal with numerically unstable points, such as in the vicinity of a vanishing Gram determinant; to allow for a sampling of the numerator function based on real values of the integration momentum; to optimize the numerical reduction in the case of long …

A New Mouse Model To Explore Therapies For Preeclampsia, Abdulwahab Ahmed, Jameel Singh, Ysodra Khan, Surya V. Seshan, Guillermina Girardi

Publications and Research

Background Pre-eclampsia, a pregnancy-specific multisystemic disorder is a leading cause of maternal and perinatal mortality and morbidity. This syndrome has been known to medical science since ancient times. However, despite considerable research, the cause/s of preeclampsia remain unclear, and there is no effective treatment. Development of an animal model that recapitulates this complex pregnancy-related disorder may help to expand our understanding and may hold great potential for the design and implementation of effective treatment. Methodology/Principal Findings Here we show that the CBA/J x DBA/2 mouse model of recurrent miscarriage is also a model of immunologically-mediated preeclampsia (PE). DBA/J mated CBA/J …

The Role Of Accounting In The Financial Crisis: Lessons For The Future, S.P. Kothari

Publications and Research

No abstract provided.

An Application Of Statistical Technique To Correct Satellite Data Due To Orbit Degradation, Md Zahidur Rahman, Leonid Roytman, Run Jesmin

Publications and Research

This paper apply an statistical technique to correct radiometric data measured by Advanced Very High Resolution Radiometers(AVHRR) onboard the National Oceanic and Atmospheric Administration (NOAA) Polar Orbiting Environmental Satellites(POES). This paper study Normalized Difference Vegetation Index (NDVI) stability in the NOAA/NESDIS Global Vegetation Index (GVI) data for the period 1982-2003. AVHRR weekly data for the five NOAA afternoon satellites NOAA-7, NOAA-9, NOAA-11, NOAA-14, and NOAA-16 are used for the China dataset, for it includes a wide variety or different ecosystems represented globally. GVI has found wide use for studying and monitoring land surface, atmosphere, and recently for analyzing climate and …

The Geography Of Black Candidate Electoral Success, Marcus Allen, Marvin King

Publications and Research

In this manuscript, we reexamine claims about the geography of electoral success of African American candidates. Barack Obama's historic election in 2008 prompted journalists, partisans, and scholars to review prior notions of where African American candidates can successfully contend for elected office. Although Obama's victory is just an anecdotal national example (albeit an important one), we review the available evidence at the state level to understand what factors might impede African American electoral success. Heretofore, the literature focused on the density of the black population, and the interconnectedness of region and white racial attitudes. This paper shows that these old …

Global Uncertainty May Moderate Trade Growth: Moving Forward As Developed Economies Slow, Eugene Spruck

Publications and Research

In this paper, Eugene Spruck, a Weissman Center Fellow who served as the Chief Economist of the Port Authority of New York and New Jersey for more than a decade, reviews economic activity in the NY/NJ metropolitan area in 2009. Based on a review of available data, the paper concludes that the region was hit particularly hard during this period. The value of two-way international trade through the Customs District declined 23.5 percent in 2009.

Forecasting The New York State Economy With “Terraced” Vars And Coincident Indices, Eric Doviak, Sean P. Macdonald

Publications and Research

This paper introduces “Terraced” Vector Autoregressive (VAR) models, an innovative twist on traditional VAR modeling, which allows the econometrician to simultaneously forecast both exogenous and endogenous variables and the confidence intervals around those forecasts. In an application of our Terraced VAR framework, we have estimated coincident indices of economic activity for the United States, New York State and the six largest metropolitan areas of New York State and incorporated them into Terraced VARs, which forecast the unemployment rate, total non-farm employment, real wages and average hours worked in manufacturing in those regions.

Mothers In Trouble: Coping With Actual Or Pending Separation From Children Due To Incarceration, Katarzyna Celinska, Jane A. Siegel

Publications and Research

Although female offenders are the fastest growing population in prison today, relatively few studies focus on their unique experiences as mothers. In this study, the authors utilize 74 semistructured interviews with mothers before trial and during incarceration to document coping strategies employed to deal with potential or actual separation from their children. From the study data, seven strategies emerge: being a good mother, mothering from prison, role redefinition, disassociation from prisoner identity, self-transformation, planning and preparation, and self-blame. The findings show that mothers used multiple strategies and tended to employ emotion-focused and adaptive coping techniques. The policy implications are discussed.

The Trouble With Archie: Locating And Accessing Primary Sources For The Study Of The 1970s Us Sitcom, All In The Family, Kathleen Collins

Publications and Research

The US television sitcom, All in the Family was groundbreaking in its social relevance with regard to contemporary issues of race, class, gender, sexual orientation and politics, among others. The interest in All in the Family continues into the 21st century, and television historians and fans continue to seek out elusive historical video of the show. The author addresses the challenges in discovering, locating and accessing primary source visual material for its study and speculates on the future of accessibility of historical broadcasts, the impact on television studies and potential solutions. Reprinted by permission of the publisher.

Editor’S Farewell, Irvin Sam Schonfeld

Publications and Research

No abstract provided.

Molecular Signatures Of The Primitive Prostate Stem Cell Niche Reveal Novel Mesenchymal-Epithelial Signaling Pathways, Roy Blum, Rashmi Gupta, Patricia E. Burger, Christopher S. Ontiveros, Sarah N. Salm, Xiaozhong Xiong, Alexander Kamb, Holger Wesche, Lisa Marshall, Gene Cutler, Xiangyun Wang, Jiri Zavadil, David Moscatelli, E. Lynette Wilson

Publications and Research

Background Signals between stem cells and stroma are important in establishing the stem cell niche. However, very little is known about the regulation of any mammalian stem cell niche as pure isolates of stem cells and their adjacent mesenchyme are not readily available. The prostate offers a unique model to study signals between stem cells and their adjacent stroma as in the embryonic prostate stem cell niche, the urogenital sinus mesenchyme is easily separated from the epithelial stem cells. Here we investigate the distinctive molecular signals of these two stem cell compartments in a mammalian system. Methodology/Principal Findings We isolated …

Identification Of An Archaeal Presenilin-Like Intramembrane Protease, Celia Torres-Arancivia, Carolyn M. Ross, Jose Chavez, Zahra Assur, Georgia Dolios, Filippo Mancia, Iban Ubarretxena-Belandia

Publications and Research

Background: The GXGD-type diaspartyl intramembrane protease, presenilin, constitutes the catalytic core of the c-secretase multi-protein complex responsible for activating critical signaling cascades during development and for the production of b-amyloid peptides (Ab) implicated in Alzheimer’s disease. The only other known GXGD-type diaspartyl intramembrane proteases are the eukaryotic signal peptide peptidases (SPPs). The presence of presenilin-like enzymes outside eukaryots has not been demonstrated. Here we report the existence of presenilin-like GXGD-type diaspartyl intramembrane proteases in archaea.

Methodology and Principal Findings: We have employed in vitro activity assays to show that MCMJR1, a polytopic membrane protein from the archaeon Methanoculleus marisnigri JR1, …

Drug Off-Target Effects Predicted Using Structural Analysis In The Context Of A Metabolic Network Model, Roger L. Chang, Lei Xie, Philip E. Bourne, Bernhard O. Palsson

Publications and Research

Recent advances in structural bioinformatics have enabled the prediction of protein-drug off-targets based on their ligand binding sites. Concurrent developments in systems biology allow for prediction of the functional effects of system perturbations using large-scale network models. Integration of these two capabilities provides a framework for evaluating metabolic drug response phenotypes in silico. This combined approach was applied to investigate the hypertensive side effect of the cholesteryl ester transfer protein inhibitor torcetrapib in the context of human renal function. A metabolic kidney model was generated in which to simulate drug treatment. Causal drug off-targets were predicted that have previously been …

Dmyc Functions Downstream Of Yorkie To Promote The Supercompetitive Behavior Of Hippo Pathway Mutant Cells, Marcello Ziosi, Luis Alberto Baena-López, Daniela Grifoni, Francesca Froldi, Andrea Pession, Flavio Garoia, Vincenzo Trotta, Paola Bellosta, Sandro Cavicchi, Annalisa Pession

Publications and Research

Genetic analyses in Drosophila epithelia have suggested that the phenomenon of “cell competition” could participate in organ homeostasis. It has been speculated that competition between different cell populations within a growing organ might play a role as either tumor promoter or tumor suppressor, depending on the cellular context. The evolutionarily conserved Hippo (Hpo) signaling pathway regulates organ size and prevents hyperplastic disease from flies to humans by restricting the activity of the transcriptional cofactor Yorkie (yki). Recent data indicate also that mutations in several Hpo pathway members provide cells with a competitive advantage by unknown mechanisms. Here we provide insight …