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Articles 781 - 810 of 2389
Full-Text Articles in Entire DC Network
A Comprehensive Reliability-Based Framework For Corrosion Damage Monitoring And Repair Design Of Reinforced Concrete Structures, Homero Castaneda, Aydin Karsilaya, Ayman Okeil, Mahmoud Reda Taha
A Comprehensive Reliability-Based Framework For Corrosion Damage Monitoring And Repair Design Of Reinforced Concrete Structures, Homero Castaneda, Aydin Karsilaya, Ayman Okeil, Mahmoud Reda Taha
Publications
In this work, we developed a comprehensive framework for corrosion management of reinforced concrete (RC) structures. This framework includes critical steps of an effective approach to quantify the damage evolution as well as providing the timeframe for effective maintenance/repair strategies for corrosion assessment in RC structures. The framework included several activities including the use of indirect and direct inspection tools, theoretical development for damage prediction, experimental measurements and theoretical development of repair time based on reliability. The uniqueness of the framework is the integration of deterministic modeling of corrosion damage evolution by using mechanistic analysis with statistical modeling on corrosion …
Recruiting, Retaining, And Promoting For Careers At Transportation Agencies, Christofer Harper, Susan Bogus, Raghava Kommalapati, Doeun Choe
Recruiting, Retaining, And Promoting For Careers At Transportation Agencies, Christofer Harper, Susan Bogus, Raghava Kommalapati, Doeun Choe
Publications
State transportation agencies currently face complex challenges in recruiting and retaining the workforce necessary to function effectively. The situation exacerbates due to the number of employees from different generations that have to coexist with varying values, expectations, and principles. These agencies are primarily comprised of two generational groups: the baby-boomers, who are approaching retirement and occupy many managerial positions; and the millennials, who are demonstrating their interest in technology and demanding dynamism in their careers. This multiplicity of interest represents a challenge for human resources (HR) in addressing workforce issues and providing the necessary means to recruit and retain qualified …
Research Incentive Programs For Closures Of Public And Private Grade Crossings, Julius Codjoe
Research Incentive Programs For Closures Of Public And Private Grade Crossings, Julius Codjoe
Publications
Vehicle-train crashes that occur at highway-rail crossings result in injuries, fatalities, and damage to equipment. In order to reduce the number of vehicle-train crashes, Federal Railroad Administration requires states which have challenges with crossing safety, including Louisiana, to develop a State Action Plan to improve grade crossing safety. Consequently, identification of specific solutions for improving safety at crossings was included in the 2015 Louisiana Transportation Plan as one of its objectives. This study seeks to identify and evaluate current and new incentive programs that encourage closure of at-grade railroad crossings to reduce the number of potential vehicle-train collision points, and …
Observations Of Reduced Turbulence And Wave Activity In The Arctic Middle Atmosphere Following The January 2015 Sudden Stratospheric Warming, Colin C. Triplett, Jintai Li, Richard L. Collins, Gerald A. Lehmacher, Aroh Barjatya, David C. Fritts, Et. Al.
Observations Of Reduced Turbulence And Wave Activity In The Arctic Middle Atmosphere Following The January 2015 Sudden Stratospheric Warming, Colin C. Triplett, Jintai Li, Richard L. Collins, Gerald A. Lehmacher, Aroh Barjatya, David C. Fritts, Et. Al.
Publications
Measurements of turbulence and waves were made as part of the Mesosphere-Lower Thermosphere Turbulence Experiment (MTeX) on the night of 25–26 January 2015 at Poker Flat Research Range, Chatanika, Alaska (65°N, 147°W). Rocket-borne ionization gauge measurements revealed turbulence in the 70- to 88-km altitude region with energy dissipation rates between 0.1 and 24 mW/kg with an average value of 2.6 mW/kg. The eddy diffusion coefficient varied between 0.3 and 134 m2/s with an average value of 10 m2/s. Turbulence was detected around mesospheric inversion layers (MILs) in both the topside and bottomside of the MILs. These …
Bayesian Analysis Of Two Stellar Populations In Galactic Globular Clusters Ii: Ngc 5024, Ngc 5272, And Ngc 6352, Rachel Wagner-Kaiser, Ted Von Hippel, David Stenning, Elliot Robinson, Ata Sarajedini, David Van Dyk, Nathan Stein, William H. Jefferys
Bayesian Analysis Of Two Stellar Populations In Galactic Globular Clusters Ii: Ngc 5024, Ngc 5272, And Ngc 6352, Rachel Wagner-Kaiser, Ted Von Hippel, David Stenning, Elliot Robinson, Ata Sarajedini, David Van Dyk, Nathan Stein, William H. Jefferys
Publications
We use Cycle 21 Hubble Space Telescope (HST) observations and HST archival ACS Treasury observations of Galactic Globular Clusters to find and characterize two stellar populations in NGC 5024 (M53), NGC 5272 (M3), and NGC 6352. For these three clusters, both single and double-population analyses are used to determine a best fit isochrone(s). We employ a sophisticated Bayesian analysis technique to simultaneously fit the cluster parameters (age, distance, absorption, and metallicity) that characterize each cluster. For the two-population analysis, unique population level helium values are also fit to each distinct population of the cluster and the relative proportions of the …
Interaction Between Gravitational Radiation And Electromagnetic Radiation, Preston Jones, Douglas Singleton
Interaction Between Gravitational Radiation And Electromagnetic Radiation, Preston Jones, Douglas Singleton
Publications
In this review paper we investigate the connection between gravity and electromagnetism from Faraday to the present day. The particular focus is on the connection between gravitational and electromagnetic radiation. We discuss electromagnetic radiation produced when a gravitational wave passes through a magnetic field. We then discuss the interaction of electromagnetic radiation with gravitational waves via Feynman diagrams of the process graviton + graviton → photon + photon. Finally we review recent work on the vacuum production of counterpart electromagnetic radiation by gravitational waves.
Science Education Trajectories: Charting The Course For Teachers, Educators, Researchers, And Policymakers, Julie Luft, Brooke A. Whitworth, Amanda Berry, Shannon Navy, Vanessa Kind
Science Education Trajectories: Charting The Course For Teachers, Educators, Researchers, And Policymakers, Julie Luft, Brooke A. Whitworth, Amanda Berry, Shannon Navy, Vanessa Kind
Publications
Science teacher professional development is complex. Phases in a teacher’s career necessitate different professional learning opportunities. Furthermore, knowledge bases, practices, and attributes need to be cultivated during these times. For science teachers, it is not always evident how to link professional learning opportunities progressively toward different outcomes, including being a department head, teacher leader, curriculum developer, or even master teacher. In order to spur a discussion about purposeful teacher learning, we use a theory of transformative learning to examine research pertaining to the professional learning of science teachers. The result is a conceptual framework that suggests that teachers should build …
Erosion Rate Equations For Coarse- Grained Materials Using A Small Flume Testing, Ghada Ellithy, Johannes L. Wibowo, Maureen K. Corcoran
Erosion Rate Equations For Coarse- Grained Materials Using A Small Flume Testing, Ghada Ellithy, Johannes L. Wibowo, Maureen K. Corcoran
Publications
Erosion rate of soils during a levee or dam overtopping event is a major component in risk assessment evaluation of breach time and consequently in determining the downstream consequences. There is uncertainty in estimation of the erosion rate especially for coarsegrained materials that comprise the outer shell layer of dams as well as homogenous levees that are constructed of such materials. In this paper, erosion rate results are presented on three soil mixes that share the same median grain size D50 of 2 mm, the fines content varies between zero and 20%, and the gravel content between zero and 30%. …
Vortex Structures Inside Spherical Mesoscopic Superconductor Plus Magnetic Dipole, A. Ludu
Vortex Structures Inside Spherical Mesoscopic Superconductor Plus Magnetic Dipole, A. Ludu
Publications
We investigate the existence of multivortex states in a superconducting mesoscopic sphere with a magnetic dipole placed at the center. We obtain analytic solutions for the order parameter inside the sphere through the linearized Ginzburg-Landau (GL) model, coupled with mixed boundary conditions, and under regularity conditions and decoupling coordinates approximation. The solutions of the linear GL equation are obtained in terms of Heun double confluent functions, in dipole coordinates symmetry. The analyticity of the solutions and the associated eigenproblem are discussed thoroughly. We minimize the free energy for the fully nonlinear GL system by using linear combinations of linear analytic …
Testing Velocity-Dependent Cpt-Violating Gravitational Forces With Radio Pulsars, Lijing Shao, Quentin G. Bailey
Testing Velocity-Dependent Cpt-Violating Gravitational Forces With Radio Pulsars, Lijing Shao, Quentin G. Bailey
Publications
In the spirit of effective field theory, the standard-model extension (SME) provides a comprehensive framework to systematically probe the possibility of Lorentz/CPT violation. In the pure gravity sector, operators with mass dimension larger than 4, while in general being advantageous to short-range experiments, are hard to investigate with systems of astronomical size. However, there is exception if the leading-order effects are CPT-violating and velocity-dependent. Here we study the lowest-order operators in the pure gravity sector that violate the CPT symmetry with carefully chosen relativistic binary pulsar systems. Applying the existing analytical results to the dynamics of a binary orbit, we …
On The Existence Of Ionospheric Feedback Instability In The Earth’S Magnetosphere-Ionosphere System, Anatoly V. Streltsov, Evgeny V. Mishin
On The Existence Of Ionospheric Feedback Instability In The Earth’S Magnetosphere-Ionosphere System, Anatoly V. Streltsov, Evgeny V. Mishin
Publications
The ionospheric feedback instability (IFI) has been considered one of the main generation mechanisms for large-amplitude ultralow frequency waves and small-scale field-aligned currents in the auroral and subauroral regions for more than 40 years. Sydorenko and Rankin (2017, https://doi.org/10.1002/2017GL073415) have recently challenged the very existence of the IFI for any realistic geophysical conditions in the Earth's ionosphere-magnetosphere system. Because this conclusion contradicts numerous theoretical, numerical, and experimental works successfully used IFI to explain and predict results from observations for more than four decades, it deserves special attention. We show that this conclusion is mainly based on the specific ionospheric …
On The Asymmetry Between Upward And Downward Field-Aligned Currents Interacting With The Ionosphere, A. V. Streltsov
On The Asymmetry Between Upward And Downward Field-Aligned Currents Interacting With The Ionosphere, A. V. Streltsov
Publications
The paper presents results from the numerical study of the magnetosphere-ionosphere interactions driven by the large-scale electric field in the magnetically conjugate, high-latitude regions of northern and southern hemispheres. Simulations of the two-fluid MHD model demonstrate that these interactions can lead to a generation of a system of small-scale, intense field-aligned currents with a significant difference in size and amplitude between the upward and downward currents. In particular, in both hemispheres, the downward currents (where the electrons are flowing from the ionosphere) become more narrow and intense than the adjacent upward currents. At high latitudes, the field-aligned currents are closely …
Gravity's Light In The Shadow Of The Moon, Andri Gretarsson, Preston Jones, Douglas Singleton
Gravity's Light In The Shadow Of The Moon, Andri Gretarsson, Preston Jones, Douglas Singleton
Publications
In this essay we look at the possibility of vacuum production of very low frequency electromagnetic radiation from a gravitational wave background (i.e. gravity's light). We also propose that this counterpart electromagnetic radiation should be detectable by a lunar orbiting satellite which is periodically occulted by the Moon (i.e., in the shadow of the Moon). For concreteness we consider the possibility of detection of both the gravitational wave and hypothesized electromagnetic radiation counterpart from the supernova core collapse of Betelgeuse
Nurturing Faculty Buy-In For Top-Down Mandates, Emily K. Faulconer
Nurturing Faculty Buy-In For Top-Down Mandates, Emily K. Faulconer
Publications
Higher education is a bureaucracy. As such, colleges and universities require strong leaders but they also must have committed faculty members. Shared governance and transparency - arguably empty buzz words – have definitions that will vary based on who you ask. Despite the minefield, these terms are relevant when discussing change within academia.
Relating Noncommutative So(2,3)* Gravity To The Lorentz-Violating Standard-Model Extension, Quentin G. Bailey, Charles D. Lane
Relating Noncommutative So(2,3)* Gravity To The Lorentz-Violating Standard-Model Extension, Quentin G. Bailey, Charles D. Lane
Publications
We consider a model of noncommutative gravity that is based on a spacetime with broken local SO(2,3)* symmetry. We show that the torsion-free version of this model is contained within the framework of the Lorentz-violating Standard-Model Extension (SME). We analyze in detail the relation between the torsion-free, quadratic limits of the broken SO(2,3)* model and the Standard-Model Extension. As part of the analysis, we construct the relevant geometric quantities to quadratic order in the metric perturbation around a flat background.
Weighted Composition Operators On Analytic Function Spaces: Some Recent Progress, Dip Acharyya
Weighted Composition Operators On Analytic Function Spaces: Some Recent Progress, Dip Acharyya
Publications
Theory of Composition Operators is a steady point of interest for almost 100 years. While studying these operators, our general goal is to describe their operator theoretic properties in terms of the associated function symbols. In this talk, I will discuss some recent results concerning linear combinations (sums, differences, etc.) of weighted composition operators in certain spaces of Analytic functions.
A Better Way Of Farming, Stephanie Rains, Brooke A. Whitworth
A Better Way Of Farming, Stephanie Rains, Brooke A. Whitworth
Publications
No abstract provided.
Superresolution Mapping Of Energy Landscape For Single Charge Carriers In Plastic Semiconductors, Yifei Jiang, Jason Mcneill
Superresolution Mapping Of Energy Landscape For Single Charge Carriers In Plastic Semiconductors, Yifei Jiang, Jason Mcneill
Publications
The performance of conjugated polymer devices is largely dictated by charge transport processes. However, it is difficult to obtain a clear relationship between conjugated polymer structures and charge transport properties, due to the complexity of the structure and the dispersive nature of charge transport in conjugated polymers. Here, we develop a method to map the energy landscape for charge transport in conjugated polymers based on simultaneous, correlated charge carrier tracking and single-particle fluorescence spectroscopy. In nanoparticles of the conjugated polymer poly[9,9-dioctylfluorenyl-2,7-diyl)-co-1,4-benzo-{2,1′-3}-thiadiazole)], two dominant chain conformations were observed, a blue-emitting phase (λmax = 550 nm) and a …
Bridge Inspecting With Unmanned Aerial Vehicles R&D, Paul Darby, Vijaya Gopu
Bridge Inspecting With Unmanned Aerial Vehicles R&D, Paul Darby, Vijaya Gopu
Publications
The project achieves through research including literature, on site interviews, and experimentation: 1) a recommendation for a UAV-based system to practically assist in routine bridge inspection work in the State of Louisiana, 2) the identification and description of advantages, disadvantages, and limitations in the use of UAVs for routing bridge inspection work in Louisiana, and 3) provided recommendations for future work. The Yuneec H520 aircraft and its E90 camera are recommended, as is the need for a boat to be included as part of the system. The recommended system has advantages in reaching portions of the bridge that are difficult …
A Bayesian Analysis Of The Ages Of Four Open Clusters, Elizabeth Jeffery, Ted Von Hippel, David A. Van Dyk, David C. Stenning, Elliot Robinson, Nathan Sten, William H. Jefferys
A Bayesian Analysis Of The Ages Of Four Open Clusters, Elizabeth Jeffery, Ted Von Hippel, David A. Van Dyk, David C. Stenning, Elliot Robinson, Nathan Sten, William H. Jefferys
Publications
In this paper we apply a Bayesian technique to determine the best fit of stellar evolution models to find the main sequence turn off age and other cluster parameters of four intermediate-age open clusters: NGC 2360, NGC 2477, NGC 2660, and NGC 3960. Our algorithm utilizes a Markov chain Monte Carlo technique to fit these various parameters, objectively finding the best-fit isochrone for each cluster. The result is a high-precision isochrone fit. We compare these results with the those of traditional “by-eye” isochrone fitting methods. By applying this Bayesian technique to NGC 2360, NGC 2477, NGC 2660, and NGC 3960, …
Momentum Flux Spectra Of A Mountain Wave Event Over New Zealand, Katrina Bossert, David C. Fritts, Christopher J. Heale, Stephen D. Eckermann, John M. C. Plane, Jonathan B. Snively, Bifford P. Williams, Iain M. Reid, Damian J. Murphy, Andrew J. Spargo, Andrew D. Mackinnon
Momentum Flux Spectra Of A Mountain Wave Event Over New Zealand, Katrina Bossert, David C. Fritts, Christopher J. Heale, Stephen D. Eckermann, John M. C. Plane, Jonathan B. Snively, Bifford P. Williams, Iain M. Reid, Damian J. Murphy, Andrew J. Spargo, Andrew D. Mackinnon
Publications
During the Deep Propagating Gravity Wave Experiment (DEEPWAVE) 13 July 2014 research flight over the South Island of New Zealand, a multiscale spectrum of mountain waves (MWs) was observed. High-resolution measurements of sodium densities were available from ~70 to 100 km for the duration of this flight. A comprehensive technique is presented for obtaining temperature perturbations, T′, from sodium mixing ratios over a range of altitudes, and these T′ were used to calculate the momentum flux (MF) spectra with respect to horizontal wavelengths, λH, for each flight segment. Spectral analysis revealed MWs with spectral power centered at λH of ~80, …
Weighted Composition Operators On Spaces Of Analytic Functions: A Survey, Soumyadip Acharyya
Weighted Composition Operators On Spaces Of Analytic Functions: A Survey, Soumyadip Acharyya
Publications
“Pure mathematics is, in its way, the poetry of logical ideas.” - Albert Einstein. Pure mathematicians study abstract entities and structures that underlie mathematics. Although their general perspective is “math for math’s sake”, sometimes even the most abstract mathematics can have unexpected applications! Come learn some of these astonishing discoveries in the history of science and mathematics! They might make you thrilled but keep in mind real-world usage is rarely the goal behind developing a new mathematical theory.
Welcome to the world of pure mathematics! In this talk, we will focus on the theory of composition operators which is a …
Localization Effects On The Dissipation Of Gravity Wave Packets In The Upper Mesophere And Lower Thermosphere, C. J. Heale, R. L. Walterscheid, J. B. Snively
Localization Effects On The Dissipation Of Gravity Wave Packets In The Upper Mesophere And Lower Thermosphere, C. J. Heale, R. L. Walterscheid, J. B. Snively
Publications
Gravity waves not subject to breaking or filtering will dissipate due to viscosity and thermal conduction in the thermosphere. However, the evolutions of wave packets, and the altitudes they reach, are highly dependent upon the spectral content. In this paper, a 2‐D numerical model is used to investigate the effect of spatial localization (and thus spectral content) of a wave packet on its dissipation, dispersion, and spectral evolution. It is found that most wave packets launched below the thermosphere evolve to smaller central vertical wavelengths as the faster, longer vertical wavelength components reach the dissipative thermosphere and are removed first, …
Floating Active Baffles, System And Method Of Slosh Damping Comprising The Same, Dae Won Kim, Rudy L. Baum, Vijay Santhanam, Balaji Sivasubramanian, Sathya Gangadharan
Floating Active Baffles, System And Method Of Slosh Damping Comprising The Same, Dae Won Kim, Rudy L. Baum, Vijay Santhanam, Balaji Sivasubramanian, Sathya Gangadharan
Publications
This disclosure provides a system for damping slosh of a liquid within a tank, a baffle for use in the system, and a method of damping slosh using the system. The system includes a plurality of baffles. Each baffle has a body configured to substantially float upon the liquid. Each baffle also has an activation material received along at least a portion of the body. The activation material is magnetically reactive provided in a quantity sufficient to enable the body to be manipulated in the presence of a magnetic field (M). The system further includes an actuator configured to provide …
Database Review: Sao/Nasa Astrophysics Data System, Laura Pope Robbins
Database Review: Sao/Nasa Astrophysics Data System, Laura Pope Robbins
Publications
Laura Pope Robbins is an advisor for The Charleston Advisor, and the review below is included in Scholarly Commons with the permission of the publisher.
The Astrophysics Data System (ADS) is the premier Open Access research database for astronomy and astrophysics containing over 13 million bibliographic records. These records include journal articles, books, conference proceedings, historical observatory bulletins, and other gray literature. An exceptional feature of ADS is the visualizations it creates, showing author networks, overlapping citations, and frequently occurring terminology giving researchers new ways to explore the literature. It is a unique resource that is as easy for a …
Modulation Of Low-Altitude Ionospheric Upflow By Linear And Nonlinear Atmospheric Gravity Waves, M. R. Burleigh, C. J. Heale, M. D. Zettergren, J. B. Snively
Modulation Of Low-Altitude Ionospheric Upflow By Linear And Nonlinear Atmospheric Gravity Waves, M. R. Burleigh, C. J. Heale, M. D. Zettergren, J. B. Snively
Publications
This study examines how thermospheric motions due to gravity waves (GWs) drive ion upflow in the F region, modulating the topside ionosphere in a way that can contribute to ion outflow. We present incoherent scatter radar data from Sondrestrom, from 31 May 2003 which showed upflow/downflow motions, having a downward phase progression, in the field‐aligned velocity, indicating forcing by a thermospheric GW. The GW‐upflow coupling dynamics are investigated through the use of a coupled atmosphere‐ionosphere model to examine potential impacts on topside ionospheric upflow. Specifically, a sequence of simulations with varying wave amplitude is conducted to determine responses to a …
Bacteria And Competing Herbivores Weaken Top–Down And Bottom–Up Aphid Suppression, Carmen K. Blubaugh, Lynne Carpenter-Boggs, John P. Reganold, Robert N. Schaeffer, William E. Snyder
Bacteria And Competing Herbivores Weaken Top–Down And Bottom–Up Aphid Suppression, Carmen K. Blubaugh, Lynne Carpenter-Boggs, John P. Reganold, Robert N. Schaeffer, William E. Snyder
Publications
Herbivore suppression is mediated by both plant defenses and predators. In turn, plant defenses are impacted by soil fertility and interactions with soil bacteria. Measuring the relative importance of nutritional and microbial drivers of herbivore resistance has proven problematic, in part because it is difficult to manipulate soil-bacterial community composition. Here, we exploit variation in soil fertility and microbial biodiversity across 20 farms to untangle suppression of aphids (Brevicoryne brassicae) through bottom–up and top–down channels. We planted Brassica oleracea plants in soil from each farm, manipulated single and dual infestations of aphids alone or with caterpillars (Pieris …
E-Hooks Provide Guidance And A Soft Landing For The Microtubule Binding Domain Of Dynein, Nayere Tajielyato, Lin Li, Yunhui Peng, Joshua Alper, Emil Alexov
E-Hooks Provide Guidance And A Soft Landing For The Microtubule Binding Domain Of Dynein, Nayere Tajielyato, Lin Li, Yunhui Peng, Joshua Alper, Emil Alexov
Publications
Macromolecular binding is a complex process that involves sensing and approaching the binding partner, adopting the proper orientation, and performing the physical binding. We computationally investigated the role of E-hooks, which are intrinsically disordered regions (IDRs) at the C-terminus of tubulin, on dynein microtubule binding domain (MTBD) binding to the microtubule as a function of the distance between the MTBD and its binding site on the microtubule. Our results demonstrated that the contacts between E-hooks and the MTBD are dynamical; multiple negatively charted patches of amino acids on the E-hooks grab and release the same positively charged patches on the …
Identifying Weak Interdomain Interactions That Stabilize The Supertertiary Structure Of The N-Terminal Tandem Pdz Domains Of Psd-95, Inna S. Yanez Orozco, Frank A. Mindlin, Junyan Ma, Bo Wang, Brie Levesque, Matheu Spencer, Soheila Rezaei Adariani, George Hamilton, Feng Ding, Mark E. Bowen, Hugo Sanabria
Identifying Weak Interdomain Interactions That Stabilize The Supertertiary Structure Of The N-Terminal Tandem Pdz Domains Of Psd-95, Inna S. Yanez Orozco, Frank A. Mindlin, Junyan Ma, Bo Wang, Brie Levesque, Matheu Spencer, Soheila Rezaei Adariani, George Hamilton, Feng Ding, Mark E. Bowen, Hugo Sanabria
Publications
Previous studies of the N-terminal PDZ tandem from PSD-95 produced divergent models and failed to identify interdomain contacts stabilizing the structure. We used ensemble and single-molecule FRET along with replica-exchange molecular dynamics to fully characterize the energy landscape. Simulations and experiments identified two conformations: an open-like conformation with a small contact interface stabilized by salt bridges, and a closed-like conformation with a larger contact interface stabilized by surface-exposed hydrophobic residues. Both interfaces were confirmed experimentally. Proximity of interdomain contacts to the binding pockets may explain the observed coupling between conformation and binding. The low-energy barrier between conformations allows submillisecond dynamics, …
Identifying Weak Interdomain Interactions That Stabilize The Supertertiary Structure Of The N-Terminal Tandem Pdz Domains Of Psd-95, Inna S. Yanez Orozco, Frank A. Mindlin, Junyan Ma, Bo Wang, Brie Levesque, Matheu Spencer, Soheila Rezaei Adariani, George Hamilton, Feng Ding, Mark E. Bowen, Hugo Sanabria
Identifying Weak Interdomain Interactions That Stabilize The Supertertiary Structure Of The N-Terminal Tandem Pdz Domains Of Psd-95, Inna S. Yanez Orozco, Frank A. Mindlin, Junyan Ma, Bo Wang, Brie Levesque, Matheu Spencer, Soheila Rezaei Adariani, George Hamilton, Feng Ding, Mark E. Bowen, Hugo Sanabria
Publications
Previous studies of the N-terminal PDZ tandem from PSD-95 produced divergent models and failed to identify interdomain contacts stabilizing the structure. We used ensemble and single-molecule FRET along with replica-exchange molecular dynamics to fully characterize the energy landscape. Simulations and experiments identified two conformations: an open-like conformation with a small contact interface stabilized by salt bridges, and a closed-like conformation with a larger contact interface stabilized by surface-exposed hydrophobic residues. Both interfaces were confirmed experimentally. Proximity of interdomain contacts to the binding pockets may explain the observed coupling between conformation and binding. The low-energy barrier between conformations allows submillisecond dynamics, …