Digital Commons Network^™

Oncogenic Gene Expression And Epigenetic Remodeling Of Cis-Regulatory Elements In Asxl1-Mutant Chronic Myelomonocytic Leukemia, Moritz Binder, Ryan M. Carr, Terra L. Lasho, Christy M. Finke, Abhishek A. Mangaonkar, Christopher L. Pin, Kurt R. Berger, Amelia Mazzone, Sandeep Potluri, Tamas Ordog, Keith D. Robertson, David L. Marks, Martin E. Fernandez-Zapico, Alexandre Gaspar-Maia, Mrinal M. Patnaik

Paediatrics Publications

Myeloid neoplasms are clonal hematopoietic stem cell disorders driven by the sequential acquisition of recurrent genetic lesions. Truncating mutations in the chromatin remodeler ASXL1 (ASXL1MT) are associated with a high-risk disease phenotype with increased proliferation, epigenetic therapeutic resistance, and poor survival outcomes. We performed a multi-omics interrogation to define gene expression and chromatin remodeling associated with ASXL1MT in chronic myelomonocytic leukemia (CMML). ASXL1MT are associated with a loss of repressive histone methylation and increase in permissive histone methylation and acetylation in promoter regions. ASXL1MT are further associated with de novo accessibility of distal enhancers binding ETS transcription factors, targeting important …

Surgical Interventions In Velopharyngeal Dysfunction: Comparative Perceptual Speech And Nasometric Outcomes For Three Techniques, Ryan Instrum, Agnieszka Dzioba, Anne Dworschak-Stokan, Murad Husein

Paediatrics Publications

Background: The aim of this study was to evaluate speech outcomes following surgical intervention for velopharyngeal dysfunction (VPD). Perceptual speech outcome data were subsequently analyzed in conjunction with patient factors such as congenital abnormalities, presence of cleft lip and/or palate, and age of repair. We hope to aid in the eventual creation of treatment algorithms for VPD, allowing practitioners to tailor surgical technique selection to patient factors. Methods: A retrospective analysis was performed for all patients who underwent surgical correction of VPD at London Health Sciences Centre between the years 2005 and 2018. Two hundred and two consecutive VPD patients …

Clinical Utility Of Combinatorial Pharmacogenomic Testing In Depression: A Canadian Patient- And Rater-Blinded, Randomized, Controlled Trial, Arun K. Tiwari, Clement C. Zai, C. Anthony Altar, Julie Anne Tanner, Paige E. Davies, Paul Traxler, James Li, Elizabeth S. Cogan, Matthew T. Kucera, Ana Gugila, Nicole Braganza, Heather Emmerson, Gwyneth Zai, Daniel J. Müller, Robert Levitan, Stefan Kloiber, Zafiris J. Daskalakis, Benicio N. Frey, James M. Bowen, Jean Eric Tarride, Richard Tytus, Ranjith Chandrasena, Nicholas Voudouris, Valerie H. Taylor, Raymond Tempier, Verinder Sharma

Paediatrics Publications

The pharmacological treatment of depression consists of stages of trial and error, with less than 40% of patients achieving remission during first medication trial. However, in a large, randomized-controlled trial (RCT) in the U.S. (“GUIDED”), significant improvements in response and remission rates were observed in patients who received treatment guided by combinatorial pharmacogenomic testing, compared to treatment-as-usual (TAU). Here we present results from the Canadian “GAPP-MDD” RCT. This 52-week, 3-arm, multi-center, participant- and rater-blinded RCT evaluated clinical outcomes among patients with depression whose treatment was guided by combinatorial pharmacogenomic testing compared to TAU. The primary outcome was symptom improvement (change …

Genetic Evidence Supports The Development Of Slc26a9 Targeting Therapies For The Treatment Of Lung Disease, Jiafen Gong, Gengming He, Cheng Wang, Claire Bartlett, Naim Panjwani, Scott Mastromatteo, Fan Lin, Katherine Keenan, Julie Avolio, Anat Halevy, Michelle Shaw, Mohsen Esmaeili, Guillaume Côté-Maurais, Damien Adam, Stéphanie Bégin, Candice Bjornson, Mark Chilvers, Joe Reisman, April Price, Michael Parkins, Richard Van Wylick, Yves Berthiaume, Lara Bilodeau, Dimas Mateos-Corral, Daniel Hughes, Mary J. Smith

Paediatrics Publications

Over 400 variants in the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) are CF-causing. CFTR modulators target variants to improve lung function, but marked variability in response exists and current therapies do not address all CF-causing variants highlighting unmet needs. Alternative epithelial ion channel/transporters such as SLC26A9 could compensate for CFTR dysfunction, providing therapeutic targets that may benefit all individuals with CF. We investigate the relationship between rs7512462, a marker of SLC26A9 activity, and lung function pre- and post-treatment with CFTR modulators in Canadian and US CF cohorts, in the general population, and in those with chronic obstructive pulmonary disease …

Assessing Cerebral Blood Flow, Oxygenation And Cytochrome C Oxidase Stability In Preterm Infants During The First 3 Days After Birth, Ajay Rajaram, Daniel Milej, Marianne Suwalski, Lilian Kebaya, Matthew Kewin, Lawrence Yip, Sandrine De Ribaupierre, Victor Han, Mamadou Diop, Soume Bhattacharya, Keith St. Lawrence

Paediatrics Publications

A major concern with preterm birth is the risk of neurodevelopmental disability. Poor cerebral circulation leading to periods of hypoxia is believed to play a significant role in the etiology of preterm brain injury, with the first three days of life considered the period when the brain is most vulnerable. This study focused on monitoring cerebral perfusion and metabolism during the first 72 h after birth in preterm infants weighing less than 1500 g. Brain monitoring was performed by combining hyperspectral near-infrared spectroscopy to assess oxygen saturation and the oxidation state of cytochrome c oxidase (oxCCO), with diffuse correlation spectroscopy …

Implementation Of An E-Learning Course In Physical Activity And Sedentary Behavior For Pre- And In-Service Early Childhood Educators: Evaluation Of The Teach Pilot Study, Brianne A. Bruijns, Leigh M. Vanderloo, Andrew M. Johnson, Kristi B. Adamo, Shauna M. Burke, Valerie Carson, Rachel Heydon, Jennifer D. Irwin, Patti Jean Naylor, Brian W. Timmons, Patricia Tucker

Paediatrics Publications

Background: Childcare-based physical activity (PA) and sedentary behavior (SB) interventions have traditionally used in-person training to supplement early childhood educators’ (ECEs) knowledge and confidence to facilitate physically active programming for the children in their care. However, this method of delivery is resource-intensive and unable to reach a high number of ECEs. The purpose of the Training pre-service EArly CHildhood educators in PA (TEACH) pilot study was to test the implementation (e.g., fidelity, feasibility, acceptability) of an e-Learning course targeting PA and SB among a sample of pre-service (i.e., post-secondary students) and in-service (i.e., practicing) ECEs in Canada. Methods: A pre-/post-study …

Change In Pre- And In-Service Early Childhood Educators’ Knowledge, Self-Efficacy, And Intentions Following An E-Learning Course In Physical Activity And Sedentary Behaviour: A Pilot Study, Brianne A. Bruijns, Leigh M. Vanderloo, Andrew M. Johnson, Kristi B. Adamo, Shauna M. Burke, Valerie Carson, Rachel Heydon, Jennifer D. Irwin, Patti Jean Naylor, Brian W. Timmons, Patricia Tucker

Paediatrics Publications

Background: Early childhood educators (ECEs) are the primary daytime role models for many young children, and are responsible for facilitating physical activity (PA) opportunities and minimizing sedentary behaviour (SB) in childcare. However, they have reportedly received little related education in their pre-service training. The purpose of the Training pre-service EArly CHildhood educators in physical activity (TEACH) pilot study was to explore changes in pre- and in-service ECEs’ knowledge, self-efficacy, behavioural intention, and perceived behavioural control following the TEACH e-Learning course in PA and SB. Methods: Pre-service ECEs were purposefully recruited from three Canadian colleges, while in-service ECEs were recruited via …

Principles Of Dormancy Evident In High-Grade Serous Ovarian Cancer, Trevor G. Shepherd, Frederick A. Dick

Paediatrics Publications

In cancer, dormancy refers to a clinical state in which microscopic residual disease becomes non-proliferative and is largely refractory to chemotherapy. Dormancy was first described in breast cancer where disease can remain undetected for decades, ultimately leading to relapse and clinical presentation of the original malignancy. A long latency period can be explained by withdrawal from cell proliferation (cellular dormancy), or a balance between proliferation and cell death that retains low levels of residual disease (tumor mass dormancy). Research into cellular dormancy has revealed features that define this state. They include arrest of cell proliferation, altered cellular metabolism, and unique …

Avoiding The Echo-Chamber: Embracing Qualitative Research In Obstetrics And Gynecology To Amplify Patient Voices, Taryn Taylor, Andrea N. Simpson, Rohan D’Souza

Paediatrics Publications

No abstract provided.

Cortical Gyrification Morphology In Asd And Adhd: Implication For Further Similarities Or Disorder-Specific Features?, Avideh Gharehgazlou, Marlee Vandewouw, Justine Ziolkowski, Jimmy Wong, Jennifer Crosbie, Russell Schachar, Rob Nicolson, Stelios Georgiades, Elizabeth Dunne, Jürgen Harreiter, Alexandra Kautzky-Willer, Peter Damm, Elisabeth R. Mathiesen, Dorte M. Jensen, Lise Lotte Andersen, Mette Tanvig, Annunziata Lapolla, Maria G. Dalfra, Alessandra Bertolotto, Ewa Wender-Ozegowska, Agnieszka Zawiejska, David Hill, Frank J. Snoek, Judith G.M. Jelsma, Gernot Desoye

Paediatrics Publications

Shared etiological pathways are suggested in ASD and ADHD given high rates of comorbidity, phenotypic overlap and shared genetic susceptibility. Given the peak of cortical gyrification expansion and emergence of ASD and ADHD symptomology in early development, we investigated gyrification morphology in 539 children and adolescents (6-17 years of age) with ASD (n=197) and ADHD (n=96) compared to typically developing controls (n=246) using the local Gyrification Index (lGI) to provide insight into contributing etiopathological factors in these two disorders. We also examined IQ effects and functional implications of gyrification by exploring the relation between lGI and ASD and ADHD symptomatology …

O-Glcnacylation And Regulation Of Galectin-3 In Extraembryonic Endoderm Differentiation, Mohamed I. Gatie, Danielle M. Spice, Amritpal Garha, Adam Mctague, Mariam Ahmer, Alexander V. Timoshenko, Gregory M. Kelly

Paediatrics Publications

The regulation of proteins through the addition and removal of O-linked β-N-acetylglucosamine (O-GlcNAc) plays a role in many signaling events, specifically in stem cell pluripo-tency and the regulation of differentiation. However, these post-translational modifications have not been explored in extraembryonic endoderm (XEN) differentiation. Of the plethora of proteins regulated through O-GlcNAc, we explored galectin-3 as a candidate protein known to have various intracellular and extracellular functions. Based on other studies, we predicted a reduction in global O-GlcNAcylation levels and a distinct galectin expression profile in XEN cells relative to embryonic stem (ES) cells. By conducting dot blot analysis, XEN cells …

Assessment Of Surrogate Markers For Cardiovascular Disease In Familial Mediterranean Fever-Related Amyloidosis Patients Homozygous For M694v Mutation In Mefv Gene, Sezgin Sahin, Micol Romano, Ferhat Guzel, David Piskin, Dimitri Poddighe, Siren Sezer, Ozgur Kasapcopur, C. Thomas Appleton, Ilker Yilmaz, Erkan Demirkaya

Paediatrics Publications

Cardiovascular disease (CVD) remains underestimated in familial Mediterranean fever-associated AA amyloidosis (FMF-AA). We aimed to compare early markers of endothelial dysfunction and atherosclerosis in FMF-AA with a homozygous M694V mutation (Group 1 = 76 patients) in the Mediterranean fever (MEFV) gene and in patients with other genotypes (Group 2 = 93 patients). Measures of increased risk for future CVD events and endothelial dysfunction, including flow-mediated dilatation (FMD), pentraxin-3 (PTX3), and carotid intima-media thickness (cIMT), and fibroblast growth factor 23 (FGF23) as a marker of atherosclerotic vascular disease were compared between groups. The frequency of clinical FMF manifestations did not differ …

Cardiovascular Response To Postural Perturbations Of Different Intensities In Healthy Young Adults, Patrick Siedlecki, J. Kevin Shoemaker, Tanya D. Ivanova, S. Jayne Garland

Paediatrics Publications

The ability to regain control of balance is vital in limiting falls and injuries. Little is known regarding how the autonomic nervous system responds during recovery from balance perturbations of different intensities. The purpose of this study was to examine the cardiovascular response following a standing balance perturbation of varying intensities, quantify cardiac baroreflex sensitivity (cBRS) during standing perturbations, and to establish the stability of the cardiac baroreflex during quiet standing before and after balance disturbances. Twenty healthy participants experienced three different perturbation intensity conditions that each included 25 brief posteriorly-directed perturbations, 8–10 s apart. Three perturbation intensity conditions (low, …

Children’S School-Day Nutrient Intake In Ontario: A Cross-Sectional Observational Study Comparing Students’ Packed Lunches From Two School Schedules, Lisa J. Neilson, Lesley A. Macaskill, Jonathan M.H. Luk, Navreeti Sharma, Marina I. Salvadori, Jamie A. Seabrook, Paula D.N. Dworatzek

Paediatrics Publications

This study compared the caloric and nutrient values of packed lunch contents and consumption in the Balanced School Day (BSD) (two 20 min eating periods) versus the Traditional Schedule (TS) (one 20 min lunch). Foods consumed during school were assessed by direct food observation in 321 grade 3 and 4 students, aged 7–10 years, at 9 BSD and 10 TS elementary schools in Ontario. Packed lunch contents in the BSD were significantly higher than the TS in energy (3128.14 ± 1100.36 vs. 2658.98 ± 951.34 kJ, p < 0.001, respectively). Similarly, carbohydrates, total sugar, protein, fat, saturated fatty acids (SFA), calcium, iron, and sodium were significantly higher in the BSD versus TS packed lunches. Correspondingly, students in the BSD consumed significantly more energy, carbohydrates, total sugar, and SFA compared to the TS. Overall, lunches brought by students in the BSD schedule provided more energy across all macronutrients, with only a few micronutrients showing increased amounts, suggesting two 20 min eating opportunities could contribute to excess caloric intake during school, potentially contributing to the prevalence of childhood overweight and obesity in Canada. Furthermore, packed lunches in both schedules had excess amounts of nutrients of concern and much work is needed to ensure that children in Canada receive nutritious lunches at school.

Freedom From Discrimination Or Freedom To Discriminate? Discursive Tensions Within Discrimination Policies In Medical Education, Javeed Sukhera, Helly Goez, Allison Brown, Wael Haddara, Saleem Razack

Paediatrics Publications

The importance of advancing equity, diversity, and inclusion for all members of the academic medical community has gained recent attention. Academic medical organizations have attempted to increase broader representation while seeking structural reforms consistent with the goal of enhancing equity and reducing disproportionality. However, efforts remain constrained while minority groups continue to experience discrimination. In this study, the authors sought to identify and understand the discursive effects of discrimination policies within medical education. The authors assembled an archive of 22 texts consisting of publicly available discrimination and harassment policy documents in 13 Canadian medical schools that were active as of …

Insights About Cannabis And Psychosis Using Video Games For Young People With A First Episode Of Psychosis, Particularly Those From Black Racialized Communities: Protocol For A Mixed Methods Study, Suzanne Archie, Lena Palaniyappan, Andrew T. Olagunju, Natasha Johnson, Nicole Kozloff, Elham Sadeh, Andrea Bardell, Alexandra Baines, Kelly K. Anderson, Oyedeji Ayonrinde, Manuela Ferrari

Paediatrics Publications

Background: Cannabis use disorder among young people with a first episode of psychosis contributes to relapse, hospitalization, and impaired functioning. However, few studies have examined what young people with early phase psychosis, particularly those from Black racialized communities, understand or appreciate about this relationship, even though they may be at risk. There are no formally tested knowledge translation strategies that disseminate these research findings for young people with emerging psychosis from Black racialized communities. Objective: This study aims to conceptualize what young people with early phase psychosis/cannabis use disorder understand about the relationship between cannabis and psychosis, focusing on people …

Oral Health Inequality In Canada, The United States And United Kingdom, Malini Chari, Vahid Ravaghi, Wael Sabbah, Noha Gomaa, Sonica Singhal, Carlos Quiñonez

Paediatrics Publications

The objective of this study was to quantify the magnitude of absolute and relative oral health inequality in countries with similar socio-political environments, but differing oral health care systems such as Canada, the United States (US), and the United Kingdom (UK), in the first decade of the new millennium. Clinical oral health data were obtained from the Canadian Health Measures Survey 2007–2009, the National Health and Nutrition Examination Survey 2007–2008, and the Adult Dental Health Survey 2009, for Canada, the US and UK, respectively. The slope index of inequality (SII) and relative index of inequality (RII) were used to quantify …

Assessment Of Surrogate Markers For Cardiovascular Disease In Familial Mediterranean Fever-Related Amyloidosis Patients Homozygous For M694v Mutation In Mefv Gene, Sezgin Sahin, Micol Romano, Ferhat Guzel, David Piskin, Dimitri Poddighe, Siren Sezer, Ozgur Kasapcopur, C. Thomas Appleton, Ilker Yilmaz, Erkan Demirkaya

Paediatrics Publications

Cardiovascular disease (CVD) remains underestimated in familial Mediterranean fever-associated AA amyloidosis (FMF-AA). We aimed to compare early markers of endothelial dysfunction and atherosclerosis in FMF-AA with a homozygous M694V mutation (Group 1 = 76 patients) in the Mediterranean fever (MEFV) gene and in patients with other genotypes (Group 2 = 93 patients). Measures of increased risk for future CVD events and endothelial dysfunction, including flow-mediated dilatation (FMD), pentraxin-3 (PTX3), and carotid intima-media thickness (cIMT), and fibroblast growth factor 23 (FGF23) as a marker of atherosclerotic vascular disease were compared between groups. The frequency of clinical FMF manifestations did not differ …

A Distinct Metabolite Signature In Military Personnel Exposed To Repetitive Low-Level Blasts, Michael R. Miller, Alicia Dibattista, Maitray A. Patel, Mark Daley, Catherine Tenn, Ann Nakashima, Shawn G. Rhind, Oshin Vartanian, Maria Y. Shiu, Norleen Caddy, Michelle Garrett, Doug Saunders, Ingrid Smith, Rakesh Jetly, Douglas D. Fraser

Paediatrics Publications

Military Breachers and Range Staff (MBRS) are subjected to repeated sub-concussive blasts, and they often report symptoms that are consistent with a mild traumatic brain injury (mTBI). Biomarkers of blast injury would potentially aid blast injury diagnosis, surveillance and avoidance. Our objective was to identify plasma metabolite biomarkers in military personnel that were exposed to repeated low-level or sub-concussive blast overpressure. A total of 37 military members were enrolled (18 MBRS and 19 controls), with MBRS having participated in 8–20 breaching courses per year, with a maximum exposure of 6 blasts per day. The two cohorts were similar except that …

Serum Erythropoietin Levels In 696 Patients Investigated For Erythrocytosis With Jak2 Mutation Analysis, Benjamin Chin-Yee, Ian Cheong, Maxim Matyashin, Alejandro Lazo-Langner, Ian Chin-Yee, Vipin Bhayana, Pratibha Bhai, Hanxin Lin, Bekim Sadikovic, Cyrus C. Hsia

Paediatrics Publications

No abstract provided.

The Unexplored Role Of Sedentary Time And Physical Activity In Glucose And Lipid Metabolism-Related Placental Mrnas In Pregnant Women Who Are Obese: The Dali Lifestyle Randomised Controlled Trial, P. Acosta-Manzano, B. Leopold-Posch, D. Simmons, R. Devlieger, S. Galjaard, R. Corcoy, J. M. Adelantado, F. Dunne, J. Iaboni, Susan Day Fragiadakis, Leanne Ristic, Jason P. Lerch, Evdokia Anagnostou

Paediatrics Publications

Objective: We aimed to explore: (i) the association of sedentary time (ST) and physical activity (PA) during pregnancy with the placental expression of genes related to glucose and lipid metabolism in pregnant women who are obese; (ii) maternal metabolic factors mediating changes in these placental transcripts; and (iii) cord blood markers related to the mRNAs mediating neonatal adiposity. Design: Multicentre randomised controlled trial. Setting: Hospitals in nine European countries. Population: A cohort of 112 pregnant women with placental tissue. Methods: Both ST and moderate-to-vigorous PA (MVPA) levels were measured objectively using accelerometry at three time periods during pregnancy. Main outcome …

Sex-Specific Alterations In Hepatic Cholesterol Metabolism In Low Birth Weight Adult Guinea Pigs., Ousseynou Sarr, Katherine E Mathers, Christina Vanderboor, Kristina Wiggers, Aditya Devgan, Daniel B Hardy, Lin Zhao, Timothy Regnault

Paediatrics Publications

BACKGROUND: Intrauterine growth restriction and low birth weight (LBW) have been widely reported as an independent risk factor for adult hypercholesterolaemia and increased hepatic cholesterol in a sex-specific manner. However, the specific impact of uteroplacental insufficiency (UPI), a leading cause of LBW in developed world, on hepatic cholesterol metabolism in later life, is ill defined and is clinically relevant in understanding later life liver metabolic health trajectories.

METHODS: Hepatic cholesterol, transcriptome, cholesterol homoeostasis regulatory proteins, and antioxidant markers were studied in UPI-induced LBW and normal birth weight (NBW) male and female guinea pigs at 150 days.

RESULTS: Hepatic free and …

Acute Changes In Forearm Vascular Compliance During Transient Sympatho-Excitation, T. Dylan Olver, Mark B. Badrov, Matti D. Allen, Nicole S. Coverdale, J. Kevin Shoemaker

Paediatrics Publications

The study of vascular regulation often omits important information about the elastic properties of arteries under conditions of pulsatile flow. The purpose of this study was to examine the relationship between muscle sympathetic nerve activity (MSNA), vascular bed compliance, and peripheral blood flow responses in humans. We hypothesized that increases in MSNA would correlate with reductions in vascular compliance, and that changes in compliance would correspond with changes in peripheral blood flow during sympatho-excitation. MSNA (microneurography), blood pressure (Finopres), and brachial artery blood flow (Doppler ultrasound), were monitored in six healthy males at baseline and during the last 15 s …

Brain Ph Measurement Using Aacid Cest Mri Incorporating The 2 Ppm Amine Resonance, Mohammed Albatany, Susan Meakin, Robert Bartha

Paediatrics Publications

Many pathological conditions lead to altered intracellular pH (pHi) disrupting normal cellular functions. The chemical exchange saturation transfer (CEST) method, known as Amine and Amide Concentration Independent Detection (AACID), can produce image contrast that is predominantly dependent on tissue intracellular pHi. The AACID value is linearly related to the ratio of the 3.5 ppm amide CEST effect and the 2.75 ppm amine CEST effect in the physiological range. However, the amine CEST effect at 2 ppm is often more clearly defined in vivo, and may provide greater sensitivity to pH changes. The purpose of the current study was to compare …

Exercise Throughout Pregnancy In A Hospital Setting: Looking Beyond Covid-19, Ruben Barakat, Cristina Silva-Jose, Michelle F. Mottola

Paediatrics Publications

No abstract provided.

Impact Of The Covid-19 Pandemic On Access To Fertility Care: A Retrospective Study At A University-Affiliated Fertility Practice, Jennifer Sau Gee Lam, Mahvash Shere, Nickan Motamedi, George A. Vilos, Basim Abu-Rafea, Angelos G. Vilos

Paediatrics Publications

Objective: To elucidate the impact of the COVID-19 pandemic on access to fertility services. Methods: A retrospective quality improvement study was conducted at a university-affiliated fertility practice in southwestern Ontario. Annual procedural volumes for intrauterine and donor inseminations (IUI/DI), in vitro fertilization and intracytoplasmic sperm injections (IVF/ICSI), and frozen embryo transfers (FET) during the COVID-19–affected year were compared with mean annual volumes from the 2 preceding years. In addition, volumes for the same procedures were compared between the first quarter of 2021 and mean first quarter volumes from 2018 to 2019. Piecewise linear regressions were conducted to evaluate whether any …

Pannexin 3 Deletion Reduces Fat Accumulation And Inflammation In A Sex-Specific Manner, C. Brent Wakefield, Vanessa R. Lee, Danielle Johnston, Parastoo Boroumand, Nicolas J. Pillon, Samar Sayedyahossein, Brooke L. O’Donnell, Justin Tang, Rafael E. Sanchez-Pupo, Kevin J. Barr, Robert Gros, Lauren Flynn, Nica M. Borradaile, Amira Klip, Frank Beier, Silvia Penuela

Paediatrics Publications

Background: Pannexin 3 (PANX3) is a channel-forming glycoprotein that enables nutrient-induced inflammation in vitro, and genetic linkage data suggest that it regulates body mass index. Here, we characterized inflammatory and metabolic parameters in global Panx3 knockout (KO) mice in the context of forced treadmill running (FEX) and high-fat diet (HFD). Methods: C57BL/6N (WT) and KO mice were randomized to either a FEX running protocol or no running (SED) from 24 until 30 weeks of age. Body weight was measured biweekly, and body composition was measured at 24 and 30 weeks of age. Male WT and KO mice were fed a …

Best Practices For Virtual Care: A Consensus Statement From The Canadian Rheumatology Association, Claire E.H. Barber, Deborah M. Levy, Vandana Ahluwalia, Arielle Mendel, Regina Taylor-Gjevre, Tommy Gerschman, Sahil Koppikar, Konstantin Jilkine, Elizabeth Stringer, Cheryl Barnabe, Sibel Zehra Aydin, Nadia Luca, Roberta Berard, Keith Tam, Jennifer Burt, Jocelyne C. Murdoch, Graeme Zinck, Therese Lane, Jennifer Heeley, Megan Mannerow, Renee Mills, Linda Wilhelm, Nicole M.S. Hartfeld, Brent Ohata

Paediatrics Publications

Objective. To develop best practice statements for the provision of virtual care in adult and pediatric rheumatology for the Canadian Rheumatology Association's (CRA) Telehealth Working Group (TWG). Methods. Four members of the TWG representing adult, pediatric, university-based, and community rheumatology practices defined the scope of the project. A rapid literature review of existing systematic reviews, policy documents, and published literature and abstracts on the topic was conducted between April and May 2021. The review informed a candidate set of 7 statements and a supporting document. The statements were submitted to a 3-round (R) modified Delphi process with 22 panelists recruited …

Training Pre-Service Early Childhood Educators In Physical Activity (Teach): Protocol For A Quasi-Experimental Study, Patricia Tucker, Brianne A. Bruijns, Kristi B. Adamo, Shauna M. Burke, Valerie Carson, Rachel Heydon, Jennifer D. Irwin, Andrew M. Johnson, Patti Jean Naylor, Brian W. Timmons, Leigh M. Vanderloo

Paediatrics Publications

Background: Early childhood educators (ECEs) influence young children’s early uptake of positive health behaviours in childcare settings and serve as important daytime role models. As such, it is imperative that post-secondary early childhood education programs provide students with the foundational knowledge and professional training required to confidently facilitate quality active play opportunities for young children. The primary objective of the Training pre-service EArly CHildhood educators in physical activity (TEACH) study is to develop and implement an e-Learning course in physical activity and sedentary behaviour to facilitate improvements in: pre-service ECEs’ self-efficacy and knowledge to lead physical activity and outdoor play …

Parental Perspectives On The Transfer Process For Critically Ill Children, Saoirse Cameron, Anna C. Gunz

Paediatrics Publications

Objective: To understand parental stressors and identify potential stress-mitigators during interfacility transfer of critically ill children. Methods: Descriptive qualitative multi-case study using semi-structured interviews. This study involved caregivers of patients admitted to the Paediatric Critical Care Unit at Children’s Hospital, London Health Sciences Centre transported from outlying hospitals. Study participants were recruited through purposeful sampling. Interviews were recorded, transcribed verbatim and manually de-identified. Coding was performed by two independent coders using a standard method of content analysis to identify common themes. Results: Themes were identified and reached saturation after twelve interviews were completed. Children were admitted primarily from Northwestern and …