Digital Commons Network^™

Nav1.3 And Fgf14 Are Primary Determinants Of The Ttx-Sensitive Sodium Current In Mouse Adrenal Chromaffin Cells, Pedro L. Martinez-Espinosa, Chengtao Yang, Xiao-Ming Xia, Christopher J. Lingle

Open Access Publications

Adrenal chromaffin cells (CCs) in rodents express rapidly inactivating, tetrodotoxin (TTX)-sensitive sodium channels. The resulting current has generally been attributed to Nav1.7, although a possible role for Nav1.3 has also been suggested. Nav channels in rat CCs rapidly inactivate via two independent pathways which differ in their time course of recovery. One subpopulation recovers with time constants similar to traditional fast inactivation and the other ∼10-fold slower, but both pathways can act within a single homogenous population of channels. Here, we use Nav1.3 KO mice to probe the properties and molecular components of Nav current in CCs. We find that …

American Society Of Hematology 2021 Guidelines On The Use Of Anticoagulation For Thromboprophylaxis In Patients With Covid-19, Adam Cuker, Kristen M. Sanfilippo, Et Al.

Open Access Publications

No abstract provided.

Targeting Primary And Metastatic Uveal Melanoma With A G Protein Inhibitor, Michael D. Onken, Carol M. Makepeace, Kevin M. Kaltenbronn, Joelle Choi, Leonel Hernandez-Aya, Katherine N. Weilbaecher, Kisha D. Piggott, P. Kumar Rao, Carla M. Yuede, Alethia J. Dixon, Patrick Osei-Owusu, John A. Cooper, Kendall J. Blumer

Open Access Publications

No abstract provided.

Glucosamine And Chondroitin Use In Relation To C-Reactive Protein Concentration: Results By Supplement Form, Formulation, And Dose, Elizabeth D. Kantor, Kelli O'Connell, Mengmeng Du, Chao Cao, Xuehong Zhang, Dong Hoon Lee, Yin Cao, Edward L. Giovannucci

Open Access Publications

No abstract provided.

Choroid Plexus Nkcc1 Mediates Cerebrospinal Fluid Clearance During Mouse Early Postnatal Development, Huixin Xu, Yong Zhang, Michael J. Holtzman, Et Al.

Open Access Publications

No abstract provided.

Disparities In Breastfeeding Among U.S. Black Mothers: Identification Of Mechanisms, Cara B. Safon, Timothy C. Heeren, Stephen M. Kerr, Daphney Clermont, Michael J. Corwin, Eve R. Colson, Rachel Y. Moon, Ann L. Kellams, Fern R. Hauck, Margaret G. Parker

Open Access Publications

No abstract provided.

Redox-Responsive Hyaluronic Acid-Based Nanogels For The Topical Delivery Of The Visual Chromophore To Retinal Photoreceptors, Amine M. Laradji, Alexander V. Kolesnikov, Bedia B. Karakoçak, Vladimir J. Kefalov, Nathan Ravi

Open Access Publications

No abstract provided.

Multistage And Transmission-Blocking Targeted Antimalarials Discovered From The Open-Source Mmv Pandemic Response Box, Janette Reader, Natalie J. Spillman, Eva S. Istvan, Daniel E. Goldberg, Et Al.

Open Access Publications

No abstract provided.

Endoplasmic Reticulum-Associated Biomarkers For Molecular Phenotyping Of Rare Kidney Disease, Chuang Li, Ying Maggie Chen

Open Access Publications

No abstract provided.

Proteomic And Functional Mapping Of Cardiac Nav1.5 Channel Phosphorylation Sites, Maxime Lorenzini, Emily Wagner, Kiersten M. Ruff, Rohit V. Pappu, Jeanne M. Nerbonne, Jonathan R. Silva, R. Reid Townsend, Et Al

Open Access Publications

Phosphorylation of the voltage-gated Na+ (NaV) channel NaV1.5 regulates cardiac excitability, yet the phosphorylation sites regulating its function and the underlying mechanisms remain largely unknown. Using a systematic, quantitative phosphoproteomic approach, we analyzed NaV1.5 channel complexes purified from nonfailing and failing mouse left ventricles, and we identified 42 phosphorylation sites on NaV1.5. Most sites are clustered, and three of these clusters are highly phosphorylated. Analyses of phosphosilent and phosphomimetic NaV1.5 mutants revealed the roles of three phosphosites in regulating NaV1.5 channel expression and gating. The phosphorylated serines S664 and S667 regulate the voltage dependence of channel activation in a cumulative …

Time Resolved Speckle Contrast Optical Spectroscopy At Quasi-Null Source-Detector Separation For Non-Invasive Measurement Of Microvascular Blood Flow, Marco Pagliazzi, Lorenzo Colombo, Ernesto E. Vidal-Rosas, Tanja Dragojević, Veronika Parfentyeva, Joseph P. Culver, Sanathana Konugolu Venkata Sekar, Laura Di Sieno, Davide Contini, Alessandro Torricelli, Antonio Pifferi, Alberto Dalla Mora, Turgut Durduran

Open Access Publications

No abstract provided.

Measuring Activities Of Daily Living In Stroke Patients With Motion Machine Learning Algorithms: A Pilot Study, Pin-Wei Chen, Nathan A Baune, Igor Zwir, Jiayu Wang, Victoria Swamidass, Alex W K Wong

Open Access Publications

Measuring activities of daily living (ADLs) using wearable technologies may offer higher precision and granularity than the current clinical assessments for patients after stroke. This study aimed to develop and determine the accuracy of detecting different ADLs using machine-learning (ML) algorithms and wearable sensors. Eleven post-stroke patients participated in this pilot study at an ADL Simulation Lab across two study visits. We collected blocks of repeated activity ("atomic" activity) performance data to train our ML algorithms during one visit. We evaluated our ML algorithms using independent semi-naturalistic activity data collected at a separate session. We tested Decision Tree, Random Forest, …

Six-Minute Walking Test Performance Relates To Neurocognitive Abilities In Preschoolers, Shelby A Keye, Anne M Walk, Corinne N Cannavale, Samantha Iwinski, Gabriella M Mcloughlin, Linda G Steinberg, Naiman A Khan

Open Access Publications

This study investigated the relationship between six-minute walking test (6MWT) distance walked and preschool-aged children's academic abilities, and behavioral and event-related potentials (ERP) indices of cognitive control. There were 59 children (25 females; age: 5.0 ± 0.6 years) who completed a 6MWT (mean distance: 449.6 ± 82.0 m) to estimate cardiorespiratory fitness. The Woodcock Johnson Early Cognitive and Academic Development Test evaluated academic abilities. A modified Eriksen flanker, hearts and flowers task, and auditory oddball task eliciting ERPs (N2, P3) assessed cognitive control. After adjusting for adiposity, diet, and demographics, linear regressions resulted in positive relationships between 6MWT distance and …

The Dark Side Of The Gut: Virome-Host Interactions In Intestinal Homeostasis And Disease, Yuhao Li, Scott A. Handley, Megan T. Baldridge

Open Access Publications

The diverse enteric viral communities that infect microbes and the animal host collectively constitute the gut virome. Although recent advances in sequencing and analysis of metaviromes have revealed the complexity of the virome and facilitated discovery of new viruses, our understanding of the enteric virome is still incomplete. Recent studies have uncovered how virome-host interactions can contribute to beneficial or detrimental outcomes for the host. Understanding the complex interactions between enteric viruses and the intestinal immune system is a prerequisite for elucidating their role in intestinal diseases. In this review, we provide an overview of the enteric virome composition and …

Integrating Imaging And Rna-Seq Improves Outcome Prediction In Cervical Cancer, Jin Zhang, Ramachandran Rashmi, Matthew Inkman, Kay Jayachandran, Fiona Ruiz, Michael R Waters, Perry W Grigsby, Stephanie Markovina, Julie K Schwarz

Open Access Publications

Approaches using a single type of data have been applied to classify human tumors. Here we integrate imaging features and transcriptomic data using a prospectively collected tumor bank. We demonstrate that increased maximum standardized uptake value on pretreatment 18F-fluorodeoxyglucose-positron emission tomography correlates with epithelial-to-mesenchymal transition (EMT) gene expression. We derived and validated 3 major molecular groups, namely squamous epithelial, squamous mesenchymal, and adenocarcinoma, using prospectively collected institutional (n = 67) and publicly available (n = 304) data sets. Patients with tumors of the squamous mesenchymal subtype showed inferior survival outcomes compared with the other 2 molecular groups. High mesenchymal gene …

Autophagy Buffers Ras-Induced Genotoxic Stress Enabling Malignant Transformation In Keratinocytes Primed By Human Papillomavirus, Eduardo Cararo-Lopes, Matheus H. Dias, Marcelo S. Da Silva, Julianna D. Zeidler, Alexandre T. Vessoni, Marcelo S. Reis, Enrique Boccardo, Hugo A. Armelin

Open Access Publications

No abstract provided.

A Pilot Study Examining The Prognostic Utility Of Tumor Shrinkage On Cone-Beam Computed Tomography (Cbct) For Stage Iii Locally Advanced Non-Small Cell Lung Cancer Patients Treated With Definitive Chemoradiation, Kylie H Kang, Jimmy T Efird, Tarun K Podder, Yuxia Zhang, Afshin Dowlati, Mitchell Machtay, Charulata Jindal, Tithi Biswas

Open Access Publications

There has been growing interest in utilizing information from cone-beam computed tomography (CBCT) to help guide both treatment delivery and prognosis. In this assessment of locally advanced unresectable stage III non-small cell lung cancer (NSCLC) treated with definitive chemoradiation, we aimed to determine the survival advantage associated with using CBCT to measure tumor regression. Patient, tumor, and treatment characteristics were collected. The serial tumor shrinkage for each patient was determined from tumor volume contours on weekly CBCTs. Survival analysis was performed using the Kaplan-Meier technique and a Cox proportional hazards model. At least two-thirds of patients had a tumor volume …

Microbiota Signals Suppress B Lymphopoiesis With Aging In Mice, Joseph R Krambs, Darlene A Monlish, Feng Gao, Laura G Schuettpelz, Daniel C Link

Open Access Publications

Aging is associated with significant changes in hematopoiesis that include a shift from lymphopoiesis to myelopoiesis and an expansion of phenotypic hematopoietic stem cells (HSCs) with impaired self-renewal capacity and myeloid-skewed lineage differentiation. Signals from commensal flora support basal myelopoiesis in young mice; however, their contribution to hematopoietic aging is largely unknown. Here, we characterize hematopoiesis in young and middle-aged mice housed under specific pathogen free (SPF) and germ-free (GF) conditions. The marked shift from lymphopoiesis to myelopoiesis that develops during aging of SPF mice is mostly abrogated in GF mice. Compared with aged SPF mice, there is a marked …

Resting-State Functional Magnetic Resonance Imaging Networks As A Quantitative Metric For Impact Of Neurosurgical Interventions, Peter H Yang, Carl D Hacker, Bhuvic Patel, Andy G S Daniel, Eric C Leuthardt

Open Access Publications

No abstract provided.

Evaluation Of Kratom Opioid Derivatives As Potential Treatment Option For Alcohol Use Disorder, Anna M Gutridge, Soumen Chakraborty, Balazs R Varga, Elizabeth S Rhoda, Alexander R French, Arryn T Blaine, Quinten H Royer, Haoyue Cui, Jinling Yuan, Robert J Cassell, Márk Szabó, Susruta Majumdar, Richard M Van Rijn

Open Access Publications

No abstract provided.

Training Leaders To Accelerate Global Mental Health Disparities Research (Lead) Program: A Research Training Program Protocol, Ozge Sensoy Bahar, Patricia Cavazos-Rehg, Fred M Ssewamala, Betsy Abente, Laura Peer, Proscovia Nabunya, Lourdes E Soto De Laurido, Theresa S Betancourt, Arvin Bhana, Tonya Edmond

Open Access Publications

No abstract provided.

Pharmacological And Biophysical Characteristics Of Picrotoxin-Resistant, Δsubunit-Containing Gabaa Receptors, Hong-Jin Shu, Xinguo Lu, John Bracamontes, Joe Henry Steinbach, Charles F Zorumski, Steven Mennerick

Open Access Publications

GABA

Successful Treatment Of Noise-Induced Hearing Loss By Mesenchymal Stromal Cells: An Rnaseq Analysis Of Protective/Repair Pathways, Athanasia Warnecke, Jennifer Harre, Matthew Shew, Adam J Mellott, Igor Majewski, Martin Durisin, Hinrich Staecker

Open Access Publications

Mesenchymal stromal cells (MSCs) are an adult derived stem cell-like population that has been shown to mediate repair in a wide range of degenerative disorders. The protective effects of MSCs are mainly mediated by the release of growth factors and cytokines thereby modulating the diseased environment and the immune system. Within the inner ear, MSCs have been shown protective against tissue damage induced by sound and a variety of ototoxins. To better understand the mechanism of action of MSCs in the inner ear, mice were exposed to narrow band noise. After exposure, MSCs derived from human umbilical cord Wharton's jelly …

Zo-1 Intracellular Localization Organizes Immune Response In Non-Small Cell Lung Cancer, Déborah Neyrinck-Leglantier, Julien Lesage, Silvia Blacher, Arnaud Bonnomet, Walter Hunziker, Agnès Noël, Valérian Dormoy, Béatrice Nawrocki-Raby, Christine Gilles, Myriam Polette

Open Access Publications

Delocalization of zonula occludens-1 (ZO-1) from tight junctions plays a substantial role in epithelial cell plasticity observed during tumor progression.

The Worldwide Alzheimer's Disease Neuroimaging Initiative: Adni-3 Updates And Global Perspectives, Christopher J Weber, Richard J Perrin, John C Morris, Et Al

Open Access Publications

The Worldwide Alzheimer's Disease Neuroimaging Initiative (WW-ADNI) is a collaborative effort to investigate imaging and biofluid markers that can inform Alzheimer's disease treatment trials. It is a public-private partnership that spans North America, Argentina, Australia, Canada, China, Japan, Korea, Mexico, and Taiwan. In 2004, ADNI researchers began a naturalistic, longitudinal study that continues today around the globe. Through several successive phases (ADNI-1, ADNI-GO, ADNI-2, and ADNI-3), the study has fueled amyloid and tau phenotyping and refined neuroimaging methodologies. WW-ADNI researchers have successfully standardized analyses and openly share data without embargo, providing a rich data set for other investigators. On August …

Helminth-Virus Interactions: Determinants Of Coinfection Outcomes, Pritesh Desai, Michael S Diamond, Larissa B Thackray

Open Access Publications

Viral infections are often studied in model mammalian organisms under specific pathogen-free conditions. However, in nature, coinfections are common, and infection with one organism can alter host susceptibility to infection with another. Helminth parasites share a long coevolutionary history with mammalian hosts and have shaped host physiology, metabolism, immunity, and the composition of the microbiome. Published studies suggest that helminth infection can either be beneficial or detrimental during viral infection. Here, we discuss coinfection studies in mouse models and use them to define key determinants that impact outcomes, including the type of antiviral immunity, the tissue tropism of both the …

Cost Burden Of Post-Transplant Lymphoproliferative Disease Following Kidney Transplants In Medicare-Eligible Patients By Survival Status, Allyson Hart, Yoon Son Ahn, Crystal Watson, Melissa Skeans, Arie Barlev, Bryn Thompson, Vikas R Dharnidharka

Open Access Publications

AIMS AND OBJECTIVES: Patients diagnosed with post-transplant lymphoproliferative disease (PTLD) experience high mortality within the first 2 years of diagnosis; however, few data exist on the economic burden of PTLD in these patients. We determined the healthcare resource utilization (HRU) and cost burden of post-kidney transplant PTLD and evaluated how these differ by survival status.

MATERIALS AND METHODS: Utilizing data from the United States Renal Data System and the Scientific Registry of Transplant Recipients, we identified 83,818 Medicare-covered kidney transplant recipients between 2007 and 2016, of which 347 had at least one Medicare claim during the first year after diagnosis …

Discovery Of Novel Reductive Elimination Pathway For 10-Hydroxywarfarin, Dakota L Pouncey, Dustyn A Barnette, Riley W Sinnott, Sarah J Phillips, Noah R Flynn, Howard P Hendrickson, S Joshua Swamidass, Grover P Miller

Open Access Publications

Coumadin (R/S-warfarin) anticoagulant therapy is highly efficacious in preventing the formation of blood clots; however, significant inter-individual variations in response risks over or under dosing resulting in adverse bleeding events or ineffective therapy, respectively. Levels of pharmacologically active forms of the drug and metabolites depend on a diversity of metabolic pathways. Cytochromes P450 play a major role in oxidizing R- and S-warfarin to 6-, 7-, 8-, 10-, and 4'-hydroxywarfarin, and warfarin alcohols form through a minor metabolic pathway involving reduction at the C11 position. We hypothesized that due to structural similarities with warfarin, hydroxywarfarins undergo reduction, possibly impacting their pharmacological …

Perceptions Of Clinical Dental Students Toward Online Education During The Covid-19 Crisis: An Egyptian Multicenter Cross-Sectional Survey, Reham Hassan, Ayman R Khalifa, Tarek Elsewify, Mohamed G Hassan

Open Access Publications

No abstract provided.

Factors Influencing Implementation Of Blood Transfusion Recommendations In Pediatric Critical Care Units, Katherine M Steffen, Philip C Spinella, Laura M Holdsworth, Mackenzie A Ford, Grace M Lee, Steven M Asch, Enola K Proctor, Allan Doctor

Open Access Publications

No abstract provided.