Digital Commons Network^™

Development And Validation Of A Multivariable Prediction Model For Missed Hiv Health Care Provider Visits In A Large Us Clinical Cohort, April C. Pettit, Aihua Bian, Cassandra O. Schember, Peter F. Rebeiro, Jeanne C. Keruly, Kenneth H. Mayer, W. Christopher Mathews, Richard D. Moore, Heidi M. Crane, Elvin Geng, Sonia Napravnik, Bryan E. Shepherd, Michael J. Mugavero

Open Access Publications

Background: Identifying individuals at high risk of missing HIV care provider visits could support proactive intervention. Previous prediction models for missed visits have not incorporated data beyond the individual level.

Methods: We developed prediction models for missed visits among people with HIV (PWH) with ≥1 follow-up visit in the Center for AIDS Research Network of Integrated Clinical Systems from 2010 to 2016. Individual-level (medical record data and patient-reported outcomes), community-level (American Community Survey), HIV care site-level (standardized clinic leadership survey), and structural-level (HIV criminalization laws, Medicaid expansion, and state AIDS Drug Assistance Program budget) predictors were included. Models were developed …

Evaluation Of A Clinical Decision Support Strategy To Increase Seasonal Influenza Vaccination Among Hospitalized Children Before Inpatient Discharge, Evan W. Orenstein, Omar Elsayed-Ali, Swaminathan Kandaswamy, Erin Masterson, Reena Blanco, Pareen Shah, Patricia Lantis, Amy Kolwaite, Thomas E. Dawson, Edwin Ray, Christy Bryant, Srikant Iyer, Andi L. Shane, Stephanie Jernigan

Open Access Publications

Importance: Hospitalized children are at increased risk of influenza-related complications, yet influenza vaccine coverage remains low among this group. Evidence-based strategies about vaccination of vulnerable children during all health care visits are especially important during the COVID-19 pandemic.

Objective: To design and evaluate a clinical decision support (CDS) strategy to increase the proportion of eligible hospitalized children who receive a seasonal influenza vaccine prior to inpatient discharge.

Design, Setting, and Participants: This quality improvement study was conducted among children eligible for the seasonal influenza vaccine who were hospitalized in a tertiary pediatric health system providing care to more than half …

Cd16+Cd163+ Monocytes Traffic To Sites Of Inflammation During Necrotizing Enterocolitis In Premature Infants, Oluwabunmi O. Olaloye, Misty Good, Et Al

Open Access Publications

Necrotizing enterocolitis (NEC) is a severe gastrointestinal complication of prematurity. Using suspension and imaging mass cytometry coupled with single-cell RNA sequencing, we demonstrate severe inflammation in patients with NEC. NEC mucosa could be subtyped by an influx of three distinct neutrophil phenotypes (immature, newly emigrated, and aged). Furthermore, CD16+CD163+ monocytes/Mϕ, correlated with newly emigrated neutrophils, were specifically enriched in NEC mucosa, found adjacent to the blood vessels, and increased in circulation of infants with surgical NEC, suggesting trafficking from the periphery to areas of inflammation. NEC-specific monocytes/Mϕ transcribed inflammatory genes, including TREM1, IL1A, IL1B, and calprotectin, and neutrophil recruitment genes …

Targeting Angiogenesis For Fracture Nonunion Treatment In Inflammatory Disease, Cuicui Wang, Jun Ying, Xiaolei Nie, Tianhong Zhou, Ding Xiao, Guarav Swarnkar, Yousef Abu-Amer, Jianjun Guan, Jie Shen

Open Access Publications

No abstract provided.

Lesion Quantification Toolkit: A Matlab Software Tool For Estimating Grey Matter Damage And White Matter Disconnections In Patients With Focal Brain Lesions, Joseph C Griffis, Nicholas V Metcalf, Maurizio Corbetta, Gordon L Shulman

Open Access Publications

Lesion studies are an important tool for cognitive neuroscientists and neurologists. However, while brain lesion studies have traditionally aimed to localize neurological symptoms to specific anatomical loci, a growing body of evidence indicates that neurological diseases such as stroke are best conceptualized as brain network disorders. While researchers in the fields of neuroscience and neurology are therefore increasingly interested in quantifying the effects of focal brain lesions on the white matter connections that form the brain's structural connectome, few dedicated tools exist to facilitate this endeavor. Here, we present the Lesion Quantification Toolkit, a publicly available MATLAB software package for …

Brain/Minds Beyond Human Brain Mri Project: A Protocol For Multi-Level Harmonization Across Brain Disorders Throughout The Lifespan, Shinsuke Koike, Matthew F Glasser, David C Van Essen, Et Al

Open Access Publications

Psychiatric and neurological disorders are afflictions of the brain that can affect individuals throughout their lifespan. Many brain magnetic resonance imaging (MRI) studies have been conducted; however, imaging-based biomarkers are not yet well established for diagnostic and therapeutic use. This article describes an outline of the planned study, the Brain/MINDS Beyond human brain MRI project (BMB-HBM, FY2018 ~ FY2023), which aims to establish clinically-relevant imaging biomarkers with multi-site harmonization by collecting data from healthy traveling subjects (TS) at 13 research sites. Collection of data in psychiatric and neurological disorders across the lifespan is also scheduled at 13 sites, whereas designing …

Phase 3 Randomized Trial Of Chemotherapy With Or Without Oblimersen In Older Aml Patients: Calgb 10201 (Alliance), Alison R. Walker, Ravi Vij, Et Al.

Open Access Publications

No abstract provided.

Self-Reported Positive Impact Of Mentored Clinical Research Training Is Associated With Academic Success In Hematology, Allison A. King, Sara K. Vesely, Grace Dadzie, Cecelia Calhoun, Adam Cuker, Wendy Stock, Alison Walker, Josel Fritz, Lilian Sung

Open Access Publications

No abstract provided.

The Extensive And Functionally Uncharacterized Mitochondrial Phosphoproteome, Natalie M. Niemi, David J. Pagliarini

Open Access Publications

No abstract provided.

Extracorporeal Support Prognostication-Time To Move The Goal Posts?, Neel Shah, Ahmed S Said

Open Access Publications

Advances in extracorporeal membrane oxygenation (ECMO) technology are associated with expanded indications, increased utilization and improved outcome. There is growing interest in developing ECMO prognostication scores to aid in bedside decision making. To date, the majority of available scores have been limited to mostly registry-based data and with mortality as the main outcome of interest. There continues to be a gap in clinically applicable decision support tools to aid in the timing of ECMO cannulation to improve patients' long-term outcomes. We present a brief review of the commonly available adult and pediatric ECMO prognostication tools, their limitations, and future directions.

Oasis/Creb3l1 Is A Factor That Responds To Nuclear Envelope Stress, Yasunao Kamikawa, Atsushi Saito, Koji Matsuhisa, Masayuki Kaneko, Rie Asada, Yasunori Horikoshi, Satoshi Tashiro, Kazunori Imaizumi

Open Access Publications

No abstract provided.

Genetic Association Study Of Childhood Aggression Across Raters, Instruments, And Age, Hill F. Ip, Andrew C. Heath, Pamela Madden, Et Al.

Open Access Publications

No abstract provided.

Report From The 6th International Meeting On Bone Marrow Adiposity (Bma2020), Erica L Scheller, Meghan E Mcgee-Lawrence, Beata Lecka-Czernik

Open Access Publications

The 6

Strategies And Tools For Studying Microglial-Mediated Synapse Elimination And Refinement, Raffaella Morini, Matteo Bizzotto, Fabio Perrucci, Fabia Filipello, Michela Matteoli

Open Access Publications

The role of microglia in controlling synapse homeostasis is becoming increasingly recognized by the scientific community. In particular, the microglia-mediated elimination of supernumerary synapses during development lays the basis for the correct formation of neuronal circuits in adulthood, while the possible reactivation of this process in pathological conditions, such as schizophrenia or Alzheimer's Disease, provides a promising target for future therapeutic strategies. The methodological approaches to investigate microglial synaptic engulfment include different

Ablative Five-Fraction Stereotactic Body Radiation Therapy For Inoperable Pancreatic Cancer Using Online Mr-Guided Adaptation, Comron Hassanzadeh, Soumon Rudra, Ani Bommireddy, William G Hawkins, Andrea Wang-Gillam, Ryan C Fields, Bin Cai, Justin Park, Olga Green, Michael Roach, Lauren Henke, Hyun Kim

Open Access Publications

Purpose: Patients with inoperable pancreatic adenocarcinoma have limited options, with traditional chemoradiation providing modest clinical benefit and an otherwise poor prognosis. Stereotactic body radiation therapy for pancreatic cancer is limited by proximity to organs-at-risk (OAR). However, stereotactic magnetic resonance-guided adaptive radiation therapy (SMART) has shown promise in delivering ablative doses safely. We sought to demonstrate the benefits of SMART using a 5-fraction approach with daily on-table adaptation.

Methods and Materials: Patients with locally advanced, nonmetastatic pancreatic adenocarcinoma were treated with 50 Gy in 5 fractions (biologically effective dose

Results: Forty-four patients were treated with SMART at our institution from 2014 …

Trends In Antimicrobial Resistance Amongst Pathogens Isolated From Blood And Cerebrospinal Fluid Cultures In Pakistan (2011-2015): A Retrospective Cross-Sectional Study, Nida Javaid, Qamar Sultana, Karam Rasool, Sumanth Gandra, Fayyaz Ahmad, Safee Ullah Chaudhary, Shaper Mirza

Open Access Publications

While antimicrobial resistance (AMR) continues to be a major public health problem in Pakistan, data regarding trends of resistance among pathogenic bacteria remains scarce, with few studies presenting long-term trends in AMR. This study was therefore designed to analyze long-term AMR trends at a national level in Pakistan. We report here results of a comprehensive analysis of resistance, among pathogens isolated from blood and cerebrospinal fluid (CSF), between 2011 and 2015. Susceptibility data was obtained from a local laboratory with collection points all across Pakistan (Chughtai Laboratory). Resistance proportions to most commonly used antimicrobials were calculated for each pathogen over …

Whole-Exome Sequencing And Hipsc Cardiomyocyte Models Identify Myrip, Trappc11, And Slc27a6 Of Potential Importance To Left Ventricular Hypertrophy In An African Ancestry Population, Marguerite R Irvin, Lisa De Las Fuentes, C Charles Gu, Karen Schwander, Dabeeru C Rao, Et Al

Open Access Publications

No abstract provided.

Sleep-Disordered Breathing In Pregnancy: A Developmental Origin Of Offspring Obesity?, Sarah S Farabi, Linda A Barbour, Teri L Hernandez

Open Access Publications

Sleep-disordered breathing (SDB) worsens over pregnancy, and obstructive sleep apnea is associated with serious maternal complications. Intrauterine exposures that provoke insulin resistance (IR), inflammation, or oxidative stress may have long-term offspring health consequences. In obesity, worsening maternal SDB appears to be an exposure that increases the risk for both small- or large-for-gestational-age (SGA, LGA, respectively), suggesting distinct outcomes linked to a common maternal phenotype. The aim of this paper is to systematically review and link data from both mechanistic rodent models and descriptive human studies to characterize the impact of maternal SDB on fetal development. A systematic review of the …

Lumbosacral Plexopathy Due To Pelvic Hematoma After Extracorporeal Membrane Oxygenation: A Case Report, Anson W. Wilks, Muhammad T. Al-Lozi

Open Access Publications

No abstract provided.

An Open Label, Block Randomized, Community Study Of The Safety And Efficacy Of Co-Administered Ivermectin, Diethylcarbamazine Plus Albendazole Vs. Diethylcarbamazine Plus Albendazole For Lymphatic Filariasis In India, Purushothaman Jambulingam, Vijesh Sreedhar Kuttiatt, Kaliannagounder Krishnamoorthy, Swaminathan Subramanian, Adinarayanan Srividya, Hari Kishan K. Raju, Manju Rahi, Roopali K. Somani, Mallanna K. Suryaprakash, Gangeshwar P. Dwivedi, Gary J Weil

Open Access Publications

No abstract provided.

Peripheral Sensory Stimulation Elicits Global Slow Waves By Recruiting Somatosensory Cortex Bilaterally, Zachary P. Rosenthal, Ryan V. Raut, Ryan M. Bowen, Abraham Z. Snyder, Joseph P. Culver, Marcus E. Raichle, Jin-Moo Lee

Open Access Publications

No abstract provided.

Single-Cell Deconvolution Of Head And Neck Squamous Cell Carcinoma, Zongtai Qi, Yating Liu, Michael Mints, Riley Mullins, Reilly Sample, Travis Law, Thomas Barrett, Angela L Mazul, Ryan S Jackson, Stephen Y Kang, Patrik Pipkorn, Anuraag S Parikh, Itay Tirosh, Joseph Dougherty, Sidharth V Puram

Open Access Publications

Complexities in cell-type composition have rightfully led to skepticism and caution in the interpretation of bulk transcriptomic analyses. Recent studies have shown that deconvolution algorithms can be utilized to computationally estimate cell-type proportions from the gene expression data of bulk blood samples, but their performance when applied to tumor tissues, including those from head and neck, remains poorly characterized. Here, we use single-cell data (~6000 single cells) collected from 21 head and neck squamous cell carcinoma (HNSCC) samples to generate cell-type-specific gene expression signatures. We leverage bulk RNA-seq data from >500 HNSCC samples profiled by The Cancer Genome Atlas (TCGA), …

A Two-Hit Model Of Alcoholic Liver Disease That Exhibits Rapid, Severe Fibrosis, Monideepa Sengupta, Suomia Abuirqeba, Amina Kameric, Aurore Cecile-Valfort, Arindam Chatterjee, Kristine Griffett, Thomas P. Burris, Colin A. Flaveny

Open Access Publications

Alcoholic liver disease (ALD) is responsible for an average of 50.4% and 44.2%of liver disease deaths among males and females respectively. Driven by alcohol misuse, ALD is often reversible by cessation of consumption. However, abstinence programs can have limited success at curtailing abuse, and the loss of life. ALD, therefore, remains a significant clinical challenge. There is a need for effective treatments that prevent or reverse alcohol-induced liver damage to complement or supplant behavioral interventions. Metabolic syndrome, which is disproportionally prevalent in ALD patients, accelerates the progression of ALD and increases liver disease mortality. Current rodent models of ALD unfortunately …

Leishmania Sexual Reproductive Strategies As Resolved Through Computational Methods Designed For Aneuploid Genomes, Jahangheer S Shaik, Deborah E Dobson, David L Sacks, Stephen M Beverley

Open Access Publications

A cryptic sexual reproductive cycle in

Factors Predicting Chronic Opioid Use After Orthopedic Surgical Procedures, Basavana Goudra, Arjun Guthal, Preet Mohinder Singh

Open Access Publications

BACKGROUND: Opioid abuse has been an increasing problem since the 1990s. With over 47,000 opioid related deaths recorded in 2017 alone, concerns have been raised regarding the dangers of introducing opioids perioperatively to patients undergoing major surgeries.

OBJECTIVES: The present study proposes to examine the frequency, amount, and trends in post-operative opioid consumption in patients undergoing orthopedic surgical procedures.

STUDY DESIGN: This was a randomized, retrospective questionnaire-based study.

SETTING: Patients who underwent any type of orthopedic surgery at the University of Pennsylvania Presbyterian Hospital from 1/1/2018 to 3/12/2019 were randomly selected and called during the summer of 2019.

METHODS: In …

Mr-Guided Radiotherapy For Liver Malignancies, Luca Boldrini, Stefanie Corradini, Cihan Gani, Lauren Henke, Ali Hosni, Angela Romano, Laura Dawson

Open Access Publications

MR guided radiotherapy represents one of the most promising recent technological innovations in the field. The possibility to better visualize therapy volumes, coupled with the innovative online adaptive radiotherapy and motion management approaches, paves the way to more efficient treatment delivery and may be translated in better clinical outcomes both in terms of response and reduced toxicity. The aim of this review is to present the existing evidence about MRgRT applications for liver malignancies, discussing the potential clinical advantages and the current pitfalls of this new technology.

Noise Exposure Potentiates Exocytosis From Cochlear Inner Hair Cells, Luis E Boero, Shelby Payne, Maria Eugenia Gómez-Casati, Mark A Rutherford, Juan D Goutman

Open Access Publications

Noise-induced hearing loss has gained relevance as one of the most common forms of hearing impairment. The anatomical correlates of hearing loss, principally cell damage and/or death, are relatively well-understood histologically. However, much less is known about the physiological aspects of damaged, surviving cells. Here we addressed the functional consequences of noise exposure on the capacity of inner hair cells (IHCs) to release synaptic vesicles at synapses with spiral ganglion neurons (SGNs). Mice of either sex at postnatal day (P) 15-16 were exposed to 1-12 kHz noise at 120 dB sound pressure level (SPL), for 1 h. Exocytosis was measured …

Development And Validation Of A Deep Learning Model To Quantify Glomerulosclerosis In Kidney Biopsy Specimens, Jon N. Marsh, Ta-Chiang Liu, Parker C. Wilson, S. Joshua Swamidass, Joseph P. Gaut

Open Access Publications

Importance: A chronic shortage of donor kidneys is compounded by a high discard rate, and this rate is directly associated with biopsy specimen evaluation, which shows poor reproducibility among pathologists. A deep learning algorithm for measuring percent global glomerulosclerosis (an important predictor of outcome) on images of kidney biopsy specimens could enable pathologists to more reproducibly and accurately quantify percent global glomerulosclerosis, potentially saving organs that would have been discarded.

Objective: To compare the performances of pathologists with a deep learning model on quantification of percent global glomerulosclerosis in whole-slide images of donor kidney biopsy specimens, and to determine the …

Fast Inactivation Of Nav Current In Rat Adrenal Chromaffin Cells Involves Two Independent Inactivation Pathways, Pedro L. Martinez-Espinosa, Alan Neely, Jiuping Ding, Christopher J. Lingle

Open Access Publications

Voltage-dependent sodium (Nav) current in adrenal chromaffin cells (CCs) is rapidly inactivating and tetrodotoxin (TTX)-sensitive. The fractional availability of CC Nav current has been implicated in regulation of action potential (AP) frequency and the occurrence of slow-wave burst firing. Here, through recordings of Nav current in rat CCs, primarily in adrenal medullary slices, we describe unique inactivation properties of CC Nav inactivation that help define AP firing rates in CCs. The key feature of CC Nav current is that recovery from inactivation, even following brief (5 ms) inactivation steps, exhibits two exponential components of similar amplitude. Various paired pulse protocols …

Sex-Dimorphic Genetic Effects And Novel Loci For Fasting Glucose And Insulin Variability, Vasiliki Lagou, Ping An, Michael A. Province, Et Al.

Open Access Publications

No abstract provided.