Digital Commons Network^™

Inadequate Antibiotic Therapy Results In Higher Recurrence Rate After Drainage Of Complicated Peri-Rectal Abscess, Rohit K. Rasane, Adrian A. Centeno Coleoglou, Christopher B. Horn, Marlon Barboza Torres, Eden Nohra, Qiao Zhang, Kelly M. Bochicchio, Obeid N. Ilahi, John E. Mazuski, Grant V. Bochicchio

Open Access Publications

No abstract provided.

Anellovirus Dynamics Are Associated With Primary Graft Dysfunction In Lung Transplantation, Joshua A. Blatter, Tsuyoshi Takahashi, Brigitte Mittler, Ruben G. Nava, Varun Puri, Daniel Kreisel, David Wang

Open Access Publications

No abstract provided.

Inhibition Of Grb14, A Negative Modulator Of Insulin Signaling, Improves Glucose Homeostasis Without Causing Cardiac Dysfunction, Xunshan Ding, Rugmani Iyer, Christopher Novotny, Daniel Metzger, Heather H. Zhou, Gordon I. Smith, Mihoko Yoshino, Jun Yoshino, Samuel Klein, Gayathri Swaminath, Saswata Talukdar, Yingjiang Zhou

Open Access Publications

No abstract provided.

Human Plasmacytoid Dendritic Cells And Cutaneous Melanoma, Matilde Monti, Francesca Consoli, Raffaella Vescovi, Mattia Bugatti, William Vermi

Open Access Publications

The prognosis of metastatic melanoma (MM) patients has remained poor for a long time. However, the recent introduction of effective target therapies (BRAF and MEK inhibitors for BRAFV600-mutated MM) and immunotherapies (anti-CTLA-4 and anti-PD-1) has significantly improved the survival of MM patients. Notably, all these responses are highly dependent on the fitness of the host immune system, including the innate compartment. Among immune cells involved in cancer immunity, properly activated plasmacytoid dendritic cells (pDCs) exert an important role, bridging the innate and adaptive immune responses and directly eliminating cancer cells. A distinctive feature of pDCs is the production of high …

Simultaneous Immunization With Omp25 And L7/L12 Provides Protection Against Brucellosis In Mice, Sonal Gupta, Surender Mohan, Vikas Kumar Somani, Somya Aggarwal, Rakesh Bhatnagar

Open Access Publications

Currently used

False-Negative Upper Extremity Ultrasound In The Initial Evaluation Of Patients With Suspected Subclavian Vein Thrombosis Due To Thoracic Outlet Syndrome (Paget-Schroetter Syndrome), Evan R Brownie, Ahmmad A Abuirqeba, J Westley Ohman, Brian G Rubin, Robert W Thompson

Open Access Publications

OBJECTIVE: To assess the utilization and consequences of upper extremity Duplex ultrasound in the initial diagnostic evaluation of patients with suspected subclavian vein (SCV) thrombosis and venous thoracic outlet syndrome (VTOS).

METHODS: A retrospective single-center review was conducted for patients that underwent primary surgical treatment for VTOS between 2008 and 2017, in whom an upper extremity ultrasound had been performed as the initial diagnostic test (n = 214). Clinical and treatment characteristics were compared between patients with positive and false-negative ultrasound studies.

RESULTS: There were 122 men (57%) and 92 women (43%) that had presented with spontaneous idiopathic arm swelling, …

Mirna-Based Rapid Differentiation Of Purified Neurons From Hpscs Advancestowards Quick Screening For Neuronal Disease Phenotypes In Vitro, Mitsuru Ishikawa, Takeshi Aoyama, Shoichiro Shibata, Takefumi Sone, Hiroyuki Miyoshi, Hirotaka Watanabe, Mari Nakamura, Saori Morota, Hiroyuki Uchino, Andrew S Yoo, Hideyuki Okano

Open Access Publications

Obtaining differentiated cells with high physiological functions by an efficient, but simple and rapid differentiation method is crucial for modeling neuronal diseases in vitro using human pluripotent stem cells (hPSCs). Currently, methods involving the transient expression of one or a couple of transcription factors have been established as techniques for inducing neuronal differentiation in a rapid, single step. It has also been reported that microRNAs can function as reprogramming effectors for directly reprogramming human dermal fibroblasts to neurons. In this study, we tested the effect of adding neuronal microRNAs, miRNA-9/9*, and miR-124 (miR-9/9*-124), for the neuronal induction method of hPSCs …

Necrotizing Soft Tissue Infections: A Focused Review Of Pathophysiology, Diagnosis, Operative Management, Antimicrobial Therapy, And Pediatrics, Jeffrey M. Tessier, James Sanders, Massimo Sartelli, Jan Ulrych, Belinda De Simone, Julia Grabowski, Sara Buckman, Therese M. Duane

Open Access Publications

No abstract provided.

Racial And Ethnic Differences In Parental Decision-Making Roles In Pediatric Oncology, Bryan A. Sisk, Tammy I. Kang, Jennifer W. Mack

Open Access Publications

No abstract provided.

Using Practice Effects For Targeted Trials Or Sub-Group Analysis In Alzheimer's Disease: How Practice Effects Predict Change Over Time, Guoqiao Wang, Richard E. Kennedy, Terry E. Goldberg, Mackenzie E. Fowler, Gary R. Cutter, Lon S. Schneider

Open Access Publications

OBJECTIVE: To describe the presence of practice effects in persons with Alzheimer disease (AD) or mild cognitive impairment (MCI) and to evaluate how practice effects affect cognitive progression and the outcome of clinical trials.

METHODS: Using data from a meta-database consisting of 18 studies including participants from the Alzheimer disease Cooperative Study (ADCS) and the Alzheimer Disease Neuroimaging Initiative (ADNI) with ADAS-Cog11 as the primary outcome, we defined practice effects based on the improvement in the first two ADAS-Cog11 scores and then estimated the presence of practice effects and compared the cognitive progression between participants with and without practice effects. …

Randomized, Double-Blind, Placebo-Controlled, Flexible-Dose Titration Study Of Methylphenidate Hydrochloride Extended-Release Capsules (Aptensio Xr) In Preschool Children With Attention-Deficit/Hyperactivity Disorder, Ann C. Childress, Scott H. Kollins, Henry C. Foehl, Jeffrey H. Newcorn, Greg Mattingly, Robert J. Kupper, Akwete L. Adjei

Open Access Publications

No abstract provided.

Cell-Specific And Athero-Protective Roles For Ripk3 In A Murine Model Of Atherosclerosis, Sarah Colijn, Vijay Muthukumar, Jun Xie, Siqi Gao, Courtney T Griffin

Open Access Publications

Receptor-interacting protein kinase 3 (RIPK3) was recently implicated in promoting atherosclerosis progression through a proposed role in macrophage necroptosis. However, RIPK3 has been connected to numerous other cellular pathways, which raises questions about its actual role in atherosclerosis. Furthermore, RIPK3 is expressed in a multitude of cell types, suggesting that it may be physiologically relevant to more than just macrophages in atherosclerosis. In this study,

Detecting Pneumonia Using Convolutions And Dynamic Capsule Routing For Chest X-Ray Images, Ansh Mittal, Deepika Kumar, Mamta Mittal, Tanzila Saba, Ibrahim Abunadi, Amjad Rehman, Sudipta Roy

Open Access Publications

An entity's existence in an image can be depicted by the activity instantiation vector from a group of neurons (called capsule). Recently, multi-layered capsules, called CapsNet, have proven to be state-of-the-art for image classification tasks. This research utilizes the prowess of this algorithm to detect pneumonia from chest X-ray (CXR) images. Here, an entity in the CXR image can help determine if the patient (whose CXR is used) is suffering from pneumonia or not. A simple model of capsules (also known as Simple CapsNet) has provided results comparable to best Deep Learning models that had been used earlier. Subsequently, a …

Composite Measure Of Physiological Dysregulation As A Predictor Of Mortality: The Long Life Family Study, Konstantin G Arbeev, Olivia Bagley, Svetlana V Ukraintseva, Hongzhe Duan, Alexander M Kulminski, Eric Stallard, Deqing Wu, Kaare Christensen, Mary F Feitosa, Bharat Thyagarajan, Joseph M Zmuda, Anatoliy I Yashin

Open Access Publications

Biological aging results in changes in an organism that accumulate over age in a complex fashion across different regulatory systems, and their cumulative effect manifests in increased physiological dysregulation (PD) and declining robustness and resilience that increase risks of health disorders and death. Several composite measures involving multiple biomarkers that capture complex effects of aging have been proposed. We applied one such approach, the Mahalanobis distance (D

Pharmacological Inhibition Of Ataxia-Telangiectasia Mutated Exacerbates Acute Kidney Injury By Activating P53 Signaling In Mice, Masahiro Uehara, Tetsuro Kusaba, Tomoharu Ida, Kunihiro Nakai, Tomohiro Nakata, Aya Tomita, Noriko Watanabe-Uehara, Kisho Ikeda, Takashi Kitani, Noriyuki Yamashita, Yuhei Kirita, Satoaki Matoba, Benjamin D. Humphreys, Keiichi Tamagaki

Open Access Publications

No abstract provided.

Anti-Angiogenic Effects Of Vegf Stimulation On Endothelium Deficient In Phosphoinositide Recycling, Amber N. Stratman, Olivia M. Farrelly, Constantinos M. Mikelis, Mayumi F. Miller, Zhiyong Wang, Van N. Pham, Andrew E. Davis, Margaret C. Burns, Sofia A. Pezoa, Daniel Castranova, Joseph J. Yano, Tina M. Kilts, George E. Davis, J. Silvio Gutkind, Brant M. Weinstein

Open Access Publications

No abstract provided.

Metabolic And Addiction Indices In Patients On Opioid Agonist Medication-Assisted Treatment: A Comparison Of Buprenorphine And Methadone, Igor Elman, Margaret Howard, Jacob T. Borodovsky, David Mysels, David Rott, David Borsook, Mark Albanese

Open Access Publications

No abstract provided.

Exploration Of Sensory And Spinal Neurons Expressing Gastrin-Releasing Peptide In Itch And Pain Related Behaviors, Devin M. Barry, Xue-Ting Liu, Benlong Liu, Xian-Yu Liu, Fang Gao, Xiansi Zeng, Juan Liu, Qianyi Yang, Steven Wilhelm, Jun Yin, Ailin Tao, Zhou-Feng Chen

Open Access Publications

No abstract provided.

Advances In Vaporisation: A Narrative Review, Pankaj Kundra, Shreya Goswami, Aruna Parameswari

Open Access Publications

No abstract provided.

Electrical Impedance Myography For Reducing Sample Size In Duchenne Muscular Dystrophy Trials, Melanie L Leitner, Kush Kapur, Basil T Darras, Michele Yang, Brenda Wong, Laura Dalle Pazze, Julaine Florence, Martin Buck, Laura Freedman, Jose Bohorquez, Seward Rutkove, Craig Zaidman

Open Access Publications

OBJECTIVE: To evaluate the sensitivity of electrical impedance myography (EIM) to disease progression in both ambulatory and non-ambulatory boys with DMD.

METHODS AND PARTICIPANTS: A non-blinded, longitudinal cohort study of 29 ambulatory and 15 non-ambulatory boys with DMD and age-similar healthy boys. Subjects were followed for up to 1 year and assessed using the Myolex

RESULTS: In the ambulatory group, EIM 100 kHz resistance values showed significant change compared to the healthy boys. For example, in lower extremity muscles, the average change in EIM 100 kHz resistance values over 12 months led to an estimated effect size of 1.58. Based …

Ascot Identifies Key Regulators Of Neuronal Subtype-Specific Splicing, Jonathan P. Ling, Christopher Wilks, Rone Charles, Patrick J. Leavey, Devlina Ghosh, Lizhi Jiang, Clayton P. Santiago, Bo Pang, Anand Venkataraman, Brian S. Clark, Abhinav Nellore, Ben Langmead, Seth Blackshaw

Open Access Publications

No abstract provided.

Structural Basis For Adhesion G Protein-Coupled Receptor Gpr126 Function, Katherine Leon, Rebecca L. Cunningham, Joshua A. Riback, Ezra Feldman, Jingxian Li, Tobin R. Sosnick, Minglei Zhao, Kelly R. Monk, Demet Araç

Open Access Publications

No abstract provided.

Innate Immune Receptor Nod2 Mediates Lgr5+ Intestinal Stem Cell Protection Against Ros Cytotoxicity Via Mitophagy Stimulation, Antonin Levy, Aline Stedman, Eric Deutsch, Françoise Donnadieu, Herbert W. Virgin, Philippe J. Sansonetti, Giulia Nigro

Open Access Publications

No abstract provided.

Dill Extract Induces Elastic Fiber Neosynthesis And Functional Improvement In The Ascending Aorta Of Aged Mice With Reversal Of Age-Dependent Cardiac Hypertrophy And Involvement Of Lysyl Oxidase-Like-1, Wassim Fhayli, Quentin Boëté, Nadjib Kihal, Valérie Cenizo, Pascal Sommer, Walter A Boyle, Marie-Paule Jacob, Gilles Faury

Open Access Publications

Elastic fibers (90% elastin, 10% fibrillin-rich microfibrils) are synthesized only in early life and adolescence mainly by the vascular smooth muscle cells through the cross-linking of its soluble precursor, tropoelastin. Elastic fibers endow the large elastic arteries with resilience and elasticity. Normal vascular aging is associated with arterial remodeling and stiffening, especially due to the end of production and degradation of elastic fibers, leading to altered cardiovascular function. Several pharmacological treatments stimulate the production of elastin and elastic fibers. In particular, dill extract (DE) has been demonstrated to stimulate elastin production in vitro in dermal equivalent models and in skin …

Metabolic Tumor Volume Response Assessment Using (11)C-Methionine Positron Emission Tomography Identifies Glioblastoma Tumor Subregions That Predict Progression Better Than Baseline Or Anatomic Magnetic Resonance Imaging Alone, Sean Miller, Matthew Schipper, Larry Junck, Morand Piert, Theodore S. Lawrence, Christina Tsien, Yue Cao, Michelle M. Kim

Open Access Publications

No abstract provided.

Rapid Determination Of Internal Strains In Soft Tissues Using An Experimentally Calibrated Finite Element Model Derived From Magnetic Resonance Imaging, Dong Hwan E. Yoon, Christian I. Weber, Garrett W. D. Easson, Kaitlyn S. Broz, Simon Y. Tang

Open Access Publications

Background: Finite element models (FEMs) of medical images can provide information about the underlying tissue that cannot be obtained from the original images. Preforming an accurate simulation requires the careful experimental calibration of boundary conditions. Here we describe a method for deriving a geometric mesh for soft biological materials using a magnetic resonance imaging (MRI) system, and an experimental workflow for calibrating the boundary conditions and optimizing the mesh density in these simulations.

Methods: A three-dimensional image stack of a ballistic sphere gel, a bovine caudal intervertebral disc (IVD), and a human lumbar IVD were generated using a positional MRI …

Induction Of Wnt16 Via Peptide-Mrna Nanoparticle-Based Delivery Maintains Cartilage Homeostasis, Huimin Yan, Ying Hu, Antonina Akk, Muhammad Farooq Rai, Hua Pan, Samuel A Wickline, Christine T N Pham

Open Access Publications

Osteoarthritis (OA) is a progressive joint disease that causes significant disability and pain and for which there are limited treatment options. We posit that delivery of anabolic factors that protect and maintain cartilage homeostasis will halt or retard OA progression. We employ a peptide-based nanoplatform to deliver Wingless and the name Int-1 (WNT) 16 messenger RNA (mRNA) to human cartilage explants. The peptide forms a self-assembled nanocomplex of approximately 65 nm in size when incubated with WNT16 mRNA. The complex is further stabilized with hyaluronic acid (HA) for enhanced cellular uptake. Delivery of peptide-WNT16 mRNA nanocomplex to human cartilage explants …

The Non-Coding Rna Landscape Of Plasma Cell Dyscrasias, Eugenio Morelli, Roberta Rocca, Kareem A Azab, Et Al

Open Access Publications

Despite substantial advancements have been done in the understanding of the pathogenesis of plasma cell (PC) disorders, these malignancies remain hard-to-treat. The discovery and subsequent characterization of non-coding transcripts, which include several members with diverse length and mode of action, has unraveled novel mechanisms of gene expression regulation often malfunctioning in cancer. Increasing evidence indicates that such non-coding molecules also feature in the pathobiology of PC dyscrasias, where they are endowed with strong therapeutic and/or prognostic potential. In this review, we aim to summarize the most relevant findings on the biological and clinical features of the non-coding RNA landscape of …

Roles Of Reconstituted High-Density Lipoprotein Nanoparticles In Cardiovascular Disease: A New Paradigm For Drug Discovery, Jiansheng Huang, Dongdong Wang, Li-Hao Huang, Hui Huang

Open Access Publications

Epidemiological results revealed that there is an inverse correlation between high-density lipoprotein (HDL) cholesterol levels and risks of atherosclerotic cardiovascular disease (ASCVD). Mounting evidence supports that HDLs are atheroprotective, therefore, many therapeutic approaches have been developed to increase HDL cholesterol (HDL-C) levels. Nevertheless, HDL-raising therapies, such as cholesteryl ester transfer protein (CETP) inhibitors, failed to ameliorate cardiovascular outcomes in clinical trials, thereby casting doubt on the treatment of cardiovascular disease (CVD) by increasing HDL-C levels. Therefore, HDL-targeted interventional studies were shifted to increasing the number of HDL particles capable of promoting ATP-binding cassette transporter A1 (ABCA1)-mediated cholesterol efflux. One such …

Patient-Controlled Intravenous Morphine Analgesia Combined With Transcranial Direct Current Stimulation For Post-Thoracotomy Pain: A Cost-Effectiveness Study And A Feasibility For Its Future Implementation, Nemanja Rancic, Katarina Mladenovic, Nela V Ilic, Viktorija Dragojevic-Simic, Menelaos Karanikolas, Tihomir V Ilic, Dusica M Stamenkovic

Open Access Publications

This prospective randomized study aims to evaluate the feasibility and cost-effectiveness of combining transcranial direct current stimulation (tDCS) with patient controlled intravenous morphine analgesia (PCA-IV) as part of multimodal analgesia after thoracotomy. Patients assigned to the active treatment group (a-tDCS,