Digital Commons Network^™

A New Gorilla Adenoviral Vector With Natural Lung Tropism Avoids Liver Toxicity And Is Amenable To Capsid Engineering And Vector Retargeting, Zhi Hong Lu, Igor P. Dmitriev, Douglas E. Brough, Elena A. Kashentseva, Jie Li, David T. Curiel

Open Access Publications

Human adenoviruses have many attractive features for gene therapy applications. However, the high prevalence of preexisting immunity against these viruses in general populations worldwide has greatly limited their clinical utility. In addition, the most commonly used human adenovirus, human adenovirus subgroup C serotype 5 (HAd5), when systemically administered, triggers systemic inflammation and toxicity, with the liver being the most severely affected organ. Here, we evaluated the utility and safety of a new low-seroprevalence gorilla adenovirus (GAd; GC46) as a gene transfer vector in mice. Biodistribution studies revealed that systemically administered GAd had a selective and robust lung endothelial cell (EC) …

A Soluble Endoplasmic Reticulum Factor As Regenerative Therapy For Wolfram Syndrome, Jana Mahadevan, Shuntaro Morikawa, Takuya Yagi, Damien Abreu, Simin Lu, Kohsuke Kanekura, Cris M Brown, Fumihiko Urano

Open Access Publications

Endoplasmic reticulum (ER) stress-mediated cell death is an emerging target for human chronic disorders, including neurodegeneration and diabetes. However, there is currently no treatment for preventing ER stress-mediated cell death. Here, we show that mesencephalic astrocyte-derived neurotrophic factor (MANF), a neurotrophic factor secreted from ER stressed cells, prevents ER stress-mediated β cell death and enhances β cell proliferation in cell and mouse models of Wolfram syndrome, a prototype of ER disorders. Our results indicate that molecular pathways regulated by MANF are promising therapeutic targets for regenerative therapy of ER stress-related disorders, including diabetes, retinal degeneration, neurodegeneration, and Wolfram syndrome.

Interleukin 27 Protects From Gastric Atrophy And Metaplasia During Chronic Autoimmune Gastritis, Kevin A Bockerstett, Christine P Petersen, Christine N Noto, Lindsey M Kuehm, Chun Fung Wong, Eric L Ford, Ryan M Teague, Jason C Mills, James R Goldenring, Richard J Dipaolo

Open Access Publications

BACKGROUND & AIMS: The association between chronic inflammation and gastric carcinogenesis is well established, but it is not clear how immune cells and cytokines regulate this process. We investigated the role of interleukin 27 (IL27) in the development of gastric atrophy, hyperplasia, and metaplasia (preneoplastic lesions associated with inflammation-induced gastric cancer) in mice with autoimmune gastritis.

METHODS: We performed studies with TxA23 mice (control mice), which express a T-cell receptor against the H+/K+ adenosine triphosphatase α chain and develop autoimmune gastritis, and TxA23xEbi3

RESULTS: We identified IL27-secreting macrophages and dendritic cell in the corpus of mice with chronic gastritis (TxA23 …

The Effects Of Vagus Nerve Stimulation On The Course And Outcomes Of Patients With Bipolar Disorder In A Treatment-Resistant Depressive Episode: A 5-Year Prospective Registry, R Hamish Mcallister-Williams, Soraia Sousa, Arun Kumar, Teresa Greco, Mark T Bunker, Scott T Aaronson, Charles R Conway, A John Rush

Open Access Publications

BACKGROUND: To compare illness characteristics, treatment history, response and durability, and suicidality scores over a 5-year period in patients with treatment-resistant bipolar depression participating in a prospective, multicenter, open-label registry and receiving Vagus Nerve Stimulation Therapy (VNS Therapy) plus treatment-as-usual (VNS + TAU) or TAU alone.

METHODS: Response was defined as ≥ 50% decrease from baseline Montgomery-Åsberg Depression Rating Scale (MADRS) total score at 3, 6, 9, or 12 months post-baseline. Response was retained while MADRS score remained ≥ 40% lower than baseline. Time-to-events was estimated using Kaplan-Meier (KM) analysis and compared using log-rank test. Suicidality was assessed using the …

Paratubal Serous Borderline Tumor In An 85 Years Old Woman: A Case Report, Jordy Mehawej, Nicole El Helou, Lina Wang, Paulette Mhawech-Fauceglia

Open Access Publications

Paratubal cysts are incidental, common and benign lesions with few cases of borderline tumor and adenocarcinoma been reported in the literature. Herein, we are discussing a case of an 85 years old woman who visited her Obstetrics and Gynecologist for a chief complaint of post-menopausal bleeding. Physical examination revealed a slightly enlarged uterus. The diagnosis of fibroids were made on ultrasound. A total hysterectomy with bilateral salpingo-oophorectomy was performed without complications. The gross examination of the specimen was most remarkable for multiple intramural fibroids and a right simple paratubal cyst meausirng 1 × 1 cm. Microscopical evaluation of the paratubal …

Effects Of Dopaminergic Drugs On Cognitive Control Processes Vary By Genotype, Daniella J Furman, Robert L White Iii, Jenna Naskolnakorn, Jean Ye, Andrew Kayser, Mark D'Esposito

Open Access Publications

Dopamine (DA) has been implicated in modulating multiple cognitive control processes, including the robust maintenance of task sets and memoranda in the face of distractors (cognitive stability) and, conversely, the ability to switch task sets or update the contents of working memory when it is advantageous to do so (cognitive flexibility). In humans, the limited specificity of available pharmacological probes has posed a challenge for understanding the mechanisms by which DA, acting on multiple receptor families across the PFC and striatum, differentially influences these cognitive processes. Using a within-subject, placebo-controlled design, we contrasted the impact of two mechanistically distinct DA …

Perceptions Of Us Adolescents And Adults With Sickle Cell Disease On Their Quality Of Care, Julie Kanter, Robert Gibson, Raymona H. Lawrence, Matthew P. Smeltzer, Norma L. Pugh, Jeffrey Glassberg, Rita V. Masese, Allison A. King, Cecelia Calhoun, Jane S. Hankins, Marsha Treadwell

Open Access Publications

Importance: Sickle cell disease (SCD) is the most common inherited red blood cell disorder in the United States, and previous studies have shown that individuals with SCD are affected by multiple health disparities, including stigmatization, inequities in funding, and worse health outcomes, which may preclude their ability to access quality health care. This needs assessment was performed as part of the Sickle Cell Disease Implementation Consortium (SCDIC) to assess barriers to care that may be faced by individuals with SCD.

Objective: To assess the SCD-related medical care experience of adolescents and adults with SCD.

Design, Setting, and Participants: This one-time …

Rodent Arena Tracker (Rat): A Machine Vision Rodent Tracking Camera And Closed Loop Control System, Jonathan Krynitsky, Alex A Legaria, Julia J Pai, Marcial Garmendia-Cedillos, Ghadi Salem, Tom Pohida, Alexxai V Kravitz

Open Access Publications

Video tracking is an essential tool in rodent research. Here, we demonstrate a machine vision rodent tracking camera based on a low-cost, open-source, machine vision camera, the OpenMV Cam M7. We call our device the rodent arena tracker (RAT), and it is a pocket-sized machine vision-based position tracker. The RAT does not require a tethered computer to operate and costs about $120 per device to build. These features make the RAT scalable to large installations and accessible to research institutions and educational settings where budgets may be limited. The RAT processes incoming video in real-time at 15 Hz and saves

Deep Learning With Diffusion Basis Spectrum Imaging For Classification Of Multiple Sclerosis Lesions, Zezhong Ye, Ajit George, Anthony T Wu, Xuan Niu, Joshua Lin, Gautam Adusumilli, Robert T Naismith, Anne H Cross, Peng Sun, Sheng-Kwei Song

Open Access Publications

OBJECTIVE: Multiple sclerosis (MS) lesions are heterogeneous with regard to inflammation, demyelination, axonal injury, and neuronal loss. We previously developed a diffusion basis spectrum imaging (DBSI) technique to better address MS lesion heterogeneity. We hypothesized that the profiles of multiple DBSI metrics can identify lesion-defining patterns. Here we test this hypothesis by combining a deep learning algorithm using deep neural network (DNN) with DBSI and other imaging methods.

METHODS: Thirty-eight MS patients were scanned with diffusion-weighted imaging, magnetization transfer imaging, and standard conventional MRI sequences (cMRI). A total of 499 regions of interest were identified on standard MRI and labeled …

Applying Two General Population Job Exposure Matrices To Predict Incident Carpal Tunnel Syndrome: A Cross-National Approach To Improve Estimation Of Workplace Physical Exposures, Marcus Yung, Bradley A Evanoff, Skye Buckner-Petty, Yves Roquelaure, Alexis Descatha, Ann Marie Dale

Open Access Publications

Objectives A job exposure matrix (JEM) is a tool to estimate workers' exposure to occupational physical risk factors. We evaluated the performance of two general population JEM (CONSTANCES and O*NET) to detect known exposure-disease relationships in an American prospective cohort study. We compared exposure estimates from three data sources and explored whether combining exposures from these two JEM, or combining exposure from each JEM with individual-level measures, improved prediction of carpal tunnel syndrome (CTS). Methods Using Cox proportional hazard models, we evaluated relationships between physical work exposure and incident CTS of 2393 workers using JEM-assigned and individual-level measure exposure information. …

Wnt Regulation: Exploring Axin-Disheveled Interactions And Defining Mechanisms By Which The Scf E3 Ubiquitin Ligase Is Recruited To The Destruction Complex, Kristina N. Schaefer, Mira I. Pronobis, Clara E. Williams, Shiping Zhang, Lauren Bauer, Dennis Goldfarb, Feng Yan, M. Ben Major, Mark Peifer

Open Access Publications

Wnt signaling plays key roles in embryonic development and adult stem cell homeostasis and is altered in human cancer. Signaling is turned on and off by regulating stability of the effector β-catenin (β-cat). The multiprotein destruction complex binds and phosphorylates β-cat and transfers it to the SCF-TrCP E3-ubiquitin ligase for ubiquitination and destruction. Wnt signals act though Dishevelled to turn down the destruction complex, stabilizing β-cat. Recent work clarified underlying mechanisms, but important questions remain. We explore β-cat transfer from the destruction complex to the E3 ligase, and test models suggesting Dishevelled and APC2 compete for association with Axin. We …

Heterotopic Pregnancy Diagnosed With Point-Of-Care Ultrasound In The Emergency Department: A Case Report, Ian J Holley, Sean P Stickles

Open Access Publications

Introduction: Heterotopic pregnancies are rare. However, they are occurring with increasing frequency. Unfortunately, diagnosis is frequently delayed, with patients presenting in extremis.

Case Report: We present a case of a heterotopic pregnancy diagnosed by point-of-care ultrasound (POCUS) in a woman presenting with lower abdominal pain, who had a documented normal first trimester ultrasound the day prior to presentation.

Discussion: Given the increasing rates of heterotopic pregnancies, we can no longer be reassured by the presence of an intra-uterine pregnancy (IUP) in a patient with concerning signs and symptoms of a ruptured ectopic pregnancy. A thorough POCUS evaluation of the uterus …

Cystic Fibrosis Foundation Consensus Guidelines For The Care Of Individuals With Advanced Cystic Fibrosis Lung Disease, Siddhartha G Kapnadak, Peter Michelson, Joan Zukosky, Et Al

Open Access Publications

BACKGROUND: Advanced cystic fibrosis lung disease (ACFLD) is common, is associated with reduced quality of life, and remains the most frequent cause of death in individuals with cystic fibrosis (CF). These consensus guidelines provide recommendations to the CF community on management of both common and unique issues that arise when individuals reach a state of ACFLD.

METHODS: The CF Foundation assembled a multidisciplinary expert panel consisting of three workgroups: Pulmonary management; Management of comorbid conditions; Symptom management and psychosocial issues. Topics were excluded if the management considerations did not differ in ACFLD from in the overall CF population or if …

Self-Care Research: How To Grow The Evidence Base?, T Jaarsma, A Strömberg, S B Dunbar, D Fitzsimons, C Lee, S Middleton, E Vellone, K E Freedland, B Riegel

Open Access Publications

BACKGROUND AND OBJECTIVE: The number of studies in the area of self-care is growing and international researchers are increasingly developing self-care interventions to improve outcomes of individual patients and communities. However, growth of the evidence is still slow due to challenges with designing and testing self-care interventions. In this article we address major methodological challenges with regard to the definition of self-care, use of theory, and research design, intended to provide guidance to researchers in this field.

METHOD: During the inaugural conference of the International Center for Self-Care Research held in Rome, Italy in June 2019 we identified important issues …

A Management Algorithm For Adult Patients With Both Brain Oxygen And Intracranial Pressure Monitoring: The Seattle International Severe Traumatic Brain Injury Consensus Conference (Sibicc), Randall Chesnut, Michael Diringer, Et Al

Open Access Publications

BACKGROUND: Current guidelines for the treatment of adult severe traumatic brain injury (sTBI) consist of high-quality evidence reports, but they are no longer accompanied by management protocols, as these require expert opinion to bridge the gap between published evidence and patient care. We aimed to establish a modern sTBI protocol for adult patients with both intracranial pressure (ICP) and brain oxygen monitors in place.

METHODS: Our consensus working group consisted of 42 experienced and actively practicing sTBI opinion leaders from six continents. Having previously established a protocol for the treatment of patients with ICP monitoring alone, we addressed patients who …

Trimerization And Genotype-Phenotype Correlation Of Col4a5 Mutants In Alport Syndrome, Misato Kamura, Tomohiko Yamamura, Kohei Omachi, Mary Ann Suico, Kandai Nozu, Shota Kaseda, Jun Kuwazuru, Tsuyoshi Shuto, Kazumoto Iijima, Hirofumi Kai

Open Access Publications

INTRODUCTION: Alport syndrome is a hereditary glomerulonephritis that results from the disruption of collagen α345(IV) heterotrimerization caused by mutation in

METHODS: We selected 9 α5(IV) missense mutants with typical glycine substitutions that clinically differed in disease progression. To quantify the trimerization of each mutant, split nanoluciferase-fused α3/α5 mutants and α4 were transfected into the cells, and intracellular and secreted heterotrimer were detected by luminescence using an assay that we developed previously.

RESULTS: Trimer formation and secretion patterns tended to be similar to the wild type in most of the mutations that did not show proteinuria at a young age. On …

Adenomyosis Presenting As A Molar Pregnancy: A Case Report, Bronwyn S Bedrick, Gregory S Kazarian, Molly M Greenwade, Nick Spies, Tiffany Chen, Horacio Maluf, Jeffrey Dicke, Premal H Thaker

Open Access Publications

•We present a case of atypical adenomyosis with clinical, laboratory, and imaging findings suggestive of a molar pregnancy.•Adenomyosis causes uterine enlargement and may appear cystic on vaginal ultrasound.•Falsely elevated β-hCG in the setting of obesity and hypothyroidism may complicate diagnosing abnormal uterine bleeding.

Intracranial Stereotactic Radiation Therapy With A Jawless Ring Gantry Linear Accelerator Equipped With New Dual Layer Multileaf Collimator, Nels C Knutson, William R Kennedy, Francisco J Reynoso, Jiayuan Peng, Lauren E Henke, Eric Laugeman, Michael Watts, Jessica Hillard, Ana Heermann, Geoffrey D Hugo, Sasa Mutic, Bin Cai

Open Access Publications

Purpose: To test the feasibility of a simplified, robust, workflow for intracranial stereotactic radiation therapy (SRT) using a ring gantry linear accelerator (RGLA) equipped with a dual-layer stacked, staggered, and interdigitating multileaf collimator.

Materials and Methods: Twenty recent clinical SRT cases treated using a radiosurgery c-arm linear accelerator were anonymized. From these data sets, a new planning workflow was developed and used to replan these cases, which then were compared to their clinical counterparts. Population-based dose-volume histograms were analyzed for target coverage and sparing of healthy brain. All plans underwent plan review and quality assurance and were delivered on an …

De Novo Identification Of Toxicants That Cause Irreparable Damage To Parasitic Nematode Intestinal Cells, Douglas P. Jasmer, Bruce A. Rosa, Rahul Tyagi, Christina A. Bulman, Brenda Beerntsen, Joseph F. Urban Jr, Judy Sakanari, Makedonka Mitreva

Open Access Publications

Efforts to identify new drugs for therapeutic and preventive treatments against parasitic nematodes have gained increasing interest with expanding pathogen omics databases and drug databases from which new anthelmintic compounds might be identified. Here, a novel approach focused on integrating a pan-Nematoda multi-omics data targeted to a specific nematode organ system (the intestinal tract) with evidence-based filtering and chemogenomic screening was undertaken. Based on de novo computational target prioritization of the 3,564 conserved intestine genes in A. suum, exocytosis was identified as a high priority pathway, and predicted inhibitors of exocytosis were tested using the large roundworm (Ascaris suum larval …

Comprehensive Analysis Of The Secreted Proteome Of Adult Necator Americanus Hookworms, Jayden Logan, Young-Jun Choi, Makedonka Mitreva, Et Al

Open Access Publications

The human hookworm Necator americanus infects more than 400 million people worldwide, contributing substantially to the poverty in these regions. Adult stage N. americanus live in the small intestine of the human host where they inject excretory/secretory (ES) products into the mucosa. ES products have been characterized at the proteome level for a number of animal hookworm species, but until now, the difficulty in obtaining sufficient live N. americanus has been an obstacle in characterizing the secretome of this important human pathogen. Herein we describe the ES proteome of N. americanus and utilize this information along with RNA Seq data …

Sucroferric Oxyhydroxide In Maintenance Hemodialysis: A Retrospective, Comparative Cohort Study, Daniel W Coyne, Linda H Ficociello, Vidhya Parameswaran, Melissa M Rosen, Claudy Mullon, Robert J Kossmann, Stuart M Sprague

Open Access Publications

RATIONALE & OBJECTIVE: High pill burden associates with reduced phosphate-binder adherence among dialysis patients, contributing to elevated serum phosphorus levels. We compared the real-world effectiveness of sucroferric oxyhydroxide (SO) versus other phosphate binders in hemodialysis patients over 2 years.

STUDY DESIGN: Retrospective cohort study.

SETTING & PARTICIPANTS: Adult in-center hemodialysis patients prescribed 2 years of uninterrupted SO therapy (maintenance SO; n = 222) compared with patients who discontinued SO therapy (discontinued SO; n = 596) within 90 days of first prescription and switched to other phosphate binder(s) for 2 years.

EXPOSURES: Phosphate binders.

OUTCOMES: Achievement of serum phosphorus levels ≤ …

Targeting Usp1-Dependent Kdm4a Protein Stability As A Potential Prostate Cancer Therapy, Shu-Zhong Cui, Zi-Ying Lei, Tian-Pei Guan, Ling-Ling Fan, You-Qiang Li, Xin-Yan Geng, De-Xue Fu, Hao-Wu Jiang, Song-Hui Xu

Open Access Publications

The histone demethylase lysine-specific demethylase 4A (KDM4A) is reported to be overexpressed and plays a vital in multiple cancers through controlling gene expression by epigenetic regulation of H3K9 or H3K36 methylation marks. However, the biological role and mechanism of KDM4A in prostate cancer (PC) remain unclear. Herein, we reported KDM4A expression was upregulation in phosphatase and tensin homolog knockout mouse prostate tissue. Depletion of KDM4A in PC cells inhibited their proliferation and survival in vivo and vitro. Further studies reveal that USP1 is a deubiquitinase that regulates KDM4A K48-linked deubiquitin and stability. Interestingly, we found c-Myc was a key downstream …

Seafood Graded Oral Food Challenge Outcomes In A Pediatric Tertiary Care Center, Zachary E Rubin, Hongjie Gu, Brooke I Polk

Open Access Publications

BACKGROUND: There are sparse data regarding the predictors of positive oral food challenges and reaction severity for seafood in children.

OBJECTIVE: Identify clinical characteristics in children with seafood allergy who were most likely to experience a negative oral food challenge (OFC).

METHODS: A retrospective chart review was performed for children who had a graded OFC to seafood at a pediatric tertiary care center from 2008 through 2019.

RESULTS: Sixty-three (60% male; average age 8 years; range 1-21 years) OFCs were performed, of which 21 were fish and 42 were shellfish. There were 10 (16%) positive OFCs and positive OFC rate …

Objective Quantification Of Image Quality And Optical Scatter Before And After Nd:Yag Capsulotomy Using A Double-Pass Technique, Abu-Bakar Zafar, Richard C Chu, Miranda N Bishara, Mujtaba A Qazi, Ashraf M Mahmoud, Jay S Pepose

Open Access Publications

Purpose: The purpose of this study is to evaluate and compare the correlation between changes in vision and HD Analyzer dual-pass metrics versus changes in vision and conventional subjective slit lamp gradings in pseudophakic patients with posterior capsular opacity undergoing neodymium:yttrium-aluminum-garnet (Nd:YAG) capsulotomy.

Patients and Methods: High contrast (HC) and low contrast (LC) best spectacle-corrected distance visual acuity (BCVA) and HD Analyzer evaluation were prospectively performed on patients with mild-to-moderate posterior capsular opacification (PCO) and monofocal and accommodating intraocular lens implants. Differences between pre- and post-operative measurements were calculated, along with the correlation of HD Analyzer metrics and slit lamp …

Delineation Of Phenotypes And Genotypes Related To Cohesin Structural Protein Rad21, Lianne C Krab, Marwan Shinawi, Et Al

Open Access Publications

RAD21 encodes a key component of the cohesin complex, and variants in RAD21 have been associated with Cornelia de Lange Syndrome (CdLS). Limited information on phenotypes attributable to RAD21 variants and genotype-phenotype relationships is currently published. We gathered a series of 49 individuals from 33 families with RAD21 alterations [24 different intragenic sequence variants (2 recurrent), 7 unique microdeletions], including 24 hitherto unpublished cases. We evaluated consequences of 12 intragenic variants by protein modelling and molecular dynamic studies. Full clinical information was available for 29 individuals. Their phenotype is an attenuated CdLS phenotype compared to that caused by variants in …

Human Monoclonal Antibodies Against Ross River Virus Target Epitopes Within The E2 Protein And Protect Against Disease, Laura A Powell, Julie M. Fox, Nurgun Kose, Arthur S. Kim, Mahsa Majedi, Robin Bombardi, Robert H. Carnahan, James C. Slaughter, Thomas E. Morrison, Michael S. Diamond, James E. Crowe

Open Access Publications

Ross River fever is a mosquito-transmitted viral disease that is endemic to Australia and the surrounding Pacific Islands. Ross River virus (RRV) belongs to the arthritogenic group of alphaviruses, which largely cause disease characterized by debilitating polyarthritis, rash, and fever. There is no specific treatment or licensed vaccine available, and the mechanisms of protective humoral immunity in humans are poorly understood. Here, we describe naturally occurring human mAbs specific to RRV, isolated from subjects with a prior natural infection. These mAbs potently neutralize RRV infectivity in cell culture and block infection through multiple mechanisms, including prevention of viral attachment, entry, …

Association Of Isolated Coronary Microvascular Dysfunction With Mortality And Major Adverse Cardiac Events: A Systematic Review And Meta-Analysis Of Aggregate Data, Mark A Gdowski, Venkatesh L Murthy, Michelle Doering, Andrea G Monroy-Gonzalez, Riemer Slart, David L Brown

Open Access Publications

Background The impact of coronary microvascular dysfunction (CMD), as diagnosed by reduced coronary flow reserve, on the outcomes of patients with symptoms of myocardial ischemia and nonobstructive coronary artery disease is poorly understood. We performed a systematic review and meta-analysis of observational studies to determine the association of CMD with outcomes. Methods and Results We searched online databases for studies where coronary flow reserve was measured invasively or noninvasively, clinical events were recorded after determination of coronary flow reserve, and the frequency of those events was reported for patients with and without CMD. The primary outcome was all-cause mortality. The …

Variation In Contrast-Associated Acute Kidney Injury Prophylaxis For Percutaneous Coronary Intervention: Insights From The Veterans Affairs Clinical Assessment, Reporting, And Tracking (Cart) Program, Joseph Walker Keach, Maggie A Stanislawski, Anna E Barón, Mary E Plomondon, Paula Langner, Amit Amin, Heather M Gilmartin, Stephen Waldo, Thomas M Maddox

Open Access Publications

BACKGROUND: Contrast-Associated Acute Kidney Injury (CA-AKI) is a serious complication associated with percutaneous coronary intervention (PCI). Patients with chronic kidney disease (CKD) have an elevated risk for developing this complication. Although CA-AKI prophylactic measures are available, the supporting literature is variable and inconsistent for periprocedural hydration and N-acetylcysteine (NAC), but is stronger for contrast minimization.

METHODS: We assessed the prevalence and variability of CA-AKI prophylaxis among CKD patients undergoing PCI between October 2007 and September 2015 in any cardiac catheterization laboratory in the VA Healthcare System. Prophylaxis included periprocedural hydration with normal saline or sodium bicarbonate, NAC, and contrast minimization …

A Mixed Methods Study Exploring Older Womens' Attitudes Toward Osteoporosis Medications: Adapting A Health Communication Framework, Lindsay N Fuzzell, Liana Fraenkel, Susan L Stark, Sarabjeet S Seehra, Christine Nelson, Audrey Keleman, Mary C Politi

Open Access Publications

No abstract provided.

The Trajectory Of Gait Development In Mice, Shyam K Akula, Katherine B Mccullough, Claire Weichselbaum, Joseph D Dougherty, Susan E Maloney

Open Access Publications

OBJECTIVE: Gait irregularities are prevalent in neurodevelopmental disorders (NDDs). However, there is a paucity of information on gait phenotypes in NDD experimental models. This is in part due to the lack of understanding of the normal developmental trajectory of gait maturation in the mouse.

MATERIALS AND METHODS: Using the DigiGait system, we have developed a quantitative, standardized, and reproducible assay of developmental gait metrics in commonly used mouse strains that can be added to the battery of mouse model phenotyping. With this assay, we characterized the trajectory of gait in the developing C57BL/6J and FVB/AntJ mouse lines.

RESULTS: In both …