Digital Commons Network^™

Pre-Clinical Common Data Elements For Traumatic Brain Injury Research: Progress And Use Cases, Michelle C. Laplaca, Stuart H. Friess, Et Al.

Open Access Publications

No abstract provided.

Functional Interaction Between Wnt And Bmp Signaling In Periosteal Bone Growth, Deye Song, Guangxu He, Yu Shi, Jiangdong Ni, Fanxin Long

Open Access Publications

No abstract provided.

Multitarget Stool Rna Test For Noninvasive Detection Of Colorectal Neoplasia In A Multicenter, Prospective, And Retrospective Cohort, Erica K. Barnell, Yiming Kang, Andrew R. Barnell, Kimberly R. Kruse, Jared Fiske, Zachary R. Pittz, Adnan R. Khan, Thomas A. Huebner, Faith L. Holmes, Malachi Griffith, Obi L. Griffith, Aadel A. Chaudhuri, Elizabeth M. Wurtzler

Open Access Publications

No abstract provided.

Interhemispheric Parietal-Frontal Connectivity Predicts The Ability To Acquire A Nondominant Hand Skill, Benjamin A. Philip, Mark P. Mcavoy, Scott H. Frey

Open Access Publications

No abstract provided.

Impact Of Intrauterine Fetal Resuscitation With Oxygen On Oxidative Stress In The Developing Rat Brain, Jia Jiang, Tusar Giri, Nandini Raghuraman, Alison G. Cahill, Arvind Palanisamy

Open Access Publications

No abstract provided.

Electronic Health Record-Embedded Individualized Pain Plans For Emergency Department Treatment Of Vaso-Occlusive Episodes In Adults With Sickle Cell Disease: Protocol For A Preimplementation And Postimplementation Study, Lingzi Luo, Allison A King, Christopher R Carpenter, Lisa Klesges, Et Al

Open Access Publications

BACKGROUND: Individuals living with sickle cell disease often require aggressive treatment of pain associated with vaso-occlusive episodes in the emergency department. Frequently, pain relief is poor. The 2014 National Heart, Lung, and Blood Institute evidence-based guidelines recommended an individualized treatment and monitoring protocol to improve pain management of vaso-occlusive episodes.

OBJECTIVE: This study will implement an electronic health record-embedded individualized pain plan with provider and patient access in the emergency departments of 8 US academic centers to improve pain treatment for adult patients with sickle cell disease. This study will assess the overall effects of electronic health record-embedded individualized pain …

A Randomized Controlled Trial Of Lactobacillus Rhamnosus Gg On Antimicrobial-Resistant Organism Colonization, Adriana M Rauseo, Tiffany Hink, Kimberly A Reske, Sondra M Seiler, Kerry M Bommarito, Victoria J Fraser, Carey-Ann D Burnham, Erik R Dubberke, Cdc Prevention Epicenter Program

Open Access Publications

OBJECTIVE: Alteration of the colonic microbiota following antimicrobial exposure allows colonization by antimicrobial-resistant organisms (AROs). Ingestion of a probiotic, such as Lactobacillus rhamnosus GG (LGG), could prevent colonization or infection with AROs by promoting healthy colonic microbiota. The purpose of this trial was to determine the effect of LGG administration on ARO colonization in hospitalized patients receiving antibiotics.

DESIGN: Prospective, double-blinded, randomized controlled trial of LGG versus placebo among patients receiving broad-spectrum antibiotics.

SETTING: Tertiary care center.

PATIENTS: In total, 88 inpatients receiving broad-spectrum antibiotics were enrolled.

INTERVENTION: Patients were randomized to receive 1 capsule containing 1×1010 cells of LGG …

Universal Masking To Control Healthcare-Associated Transmission Of Severe Acute Respiratory Coronavirus Virus 2 (Sars-Cov-2), Eliza R Thompson, Faith S Williams, Pat A Giacin, Shay Drummond, Eric Brown, Meredith Nalick, Qian Wang, Jay R Mcdonald, Abigail L Carlson

Open Access Publications

OBJECTIVES: To assess extent of a healthcare-associated outbreak of severe acute respiratory coronavirus virus 2 (SARS-CoV-2) and to evaluate the effectiveness of infection control measures, including universal masking.

DESIGN: Outbreak investigation including 4 large-scale point-prevalence surveys.

SETTING: Integrated VA healthcare system with 2 facilities and 330 beds.

PARTICIPANTS: Index patient and 250 exposed patients and staff.

METHODS: We identified exposed patients and staff and classified them as probable and confirmed cases based on symptoms and testing. We performed a field investigation and an assessment of patient and staff interactions to develop probable transmission routes. Infection prevention interventions included droplet and …

Attitudinal Acceptance Of Intimate Partner Violence And Mental Health Outcomes For Female Survivors In Sub-Saharan Africa, Reine-Marcelle Ibala, Ilana Seff, Lindsay Stark

Open Access Publications

While current literature evidences a strong association between gender-based violence exposure and adverse mental health outcomes, few studies have explored how attitudinal acceptance of intimate partner violence (IPV) might impact this relationship. This analysis employed data from 13-24-year-old females as part of the Violence Against Children Surveys in Nigeria, Uganda, and Malawi. Mental health status, defined by the Kessler Screening Scale for Psychological Distress, and suicide ideation served as outcome measures. Predictors of interest included lifetime experiences of IPV and attitudinal acceptance of IPV. Country-stratified logistic and ordinary least squares regressions were used to predict outcomes and included interactions between …

Acetylated Tau Inhibits Chaperone-Mediated Autophagy And Promotes Tau Pathology Propagation In Mice, Benjamin Caballero, Celeste M. Karch, Et Al.

Open Access Publications

No abstract provided.

Radiosynthesis And Evaluation Of Talazoparib And Its Derivatives As Parp-1-Targeting Agents, Dong Zhou, Huaping Chen, Cedric Mpoy, Sadia Afrin, Buck E Rogers, Joel R Garbow, John A Katzenellenbogen, Jinbin Xu

Open Access Publications

Poly (ADP-ribose) polymerase-1 (PARP-1) is a critical enzyme in the DNA repair process and the target of several FDA-approved inhibitors. Several of these inhibitors have been radiolabeled for non-invasive imaging of PARP-1 expression or targeted radiotherapy of PARP-1 expressing tumors. In particular, derivatives of olaparib and rucaparib, which have reduced trapping potency by PARP-1 compared to talazoparib, have been radiolabeled for these purposes. Here, we report the first radiosynthesis of [

Beam Energy Metrics For The Acceptance And Quality Assurance Of Halcyon Linear Accelerator, Song Gao, Mikhail A Chetvertkov, Bin Cai, Abhishek Dwivedi, Dimitris Mihailidis, Xenia Ray, Tucker Netherton, Laurence E Court, William E Simon, Peter A Balter

Open Access Publications

PURPOSE: Establish and compare two metrics for monitoring beam energy changes in the Halcyon platform and evaluate the accuracy of these metrics across multiple Halcyon linacs.

METHOD: The first energy metric is derived from the diagonal normalized flatness (F

RESULTS: Five Halcyon linacs from five institutions were used to evaluate the accuracy of the D

CONCLUSION: The F

Pathogenesis And Current Treatment Of Osteosarcoma: Perspectives For Future Therapies, Richa Rathore, Brian A Van Tine

Open Access Publications

Osteosarcoma is the most common primary malignant bone tumor in children and young adults. The standard-of-care curative treatment for osteosarcoma utilizes doxorubicin, cisplatin, and high-dose methotrexate, a standard that has not changed in more than 40 years. The development of patient-specific therapies requires an in-depth understanding of the unique genetics and biology of the tumor. Here, we discuss the role of normal bone biology in osteosarcomagenesis, highlighting the factors that drive normal osteoblast production, as well as abnormal osteosarcoma development. We then describe the pathology and current standard of care of osteosarcoma. Given the complex heterogeneity of osteosarcoma tumors, we …

Identification Of A Novel Genetic Marker For Risk Of Degenerative Rotator Cuff Disease Surgery In The Uk Biobank, Elizabeth L Yanik, Jay D Keener, Shiow J Lin, Graham A Colditz, Rick W Wright, Bradley A Evanoff, Nitin B Jain, Nancy L Saccone

Open Access Publications

BACKGROUND: While evidence indicates that familial predisposition influences the risk of developing degenerative rotator cuff disease (RCD), knowledge of specific genetic markers is limited. We conducted a genome-wide association study of RCD surgery using the UK Biobank, a prospective cohort of 500,000 people (40 to 69 years of age at enrollment) with genotype data.

METHODS: Cases with surgery for degenerative RCD were identified using linked hospital records. The cases were defined as an International Classification of Diseases, Tenth Revision (ICD-10) code of M75.1 determined by a trauma/orthopaedic specialist and surgery consistent with RCD treatment. Cases were excluded if a diagnosis …

Postnatal Expression Profiles Of Atypical Cadherin Fat1 Suggest Its Role In Autism, Jeannine A Frei, Cheryl J Brandenburg, Jonathan E Nestor, Didier M Hodzic, Celine Plachez, Helen Mcneill, Derek M Dykxhoorn, Michael W Nestor, Gene J Blatt, Yu-Chih Lin

Open Access Publications

Genetic studies have linked FAT1 (FAT atypical cadherin 1) with autism spectrum disorder (ASD); however, the role that FAT1 plays in ASD remains unknown. In mice, the function of Fat1 has been primarily implicated in embryonic nervous system development with less known about its role in postnatal development. We show for the first time that FAT1 protein is expressed in mouse postnatal brains and is enriched in the cerebellum, where it localizes to granule neurons and Golgi cells in the granule layer, as well as inhibitory neurons in the molecular layer. Furthermore, subcellular characterization revealed FAT1 localization in neurites and …

Modular Network Between Postrhinal Visual Cortex, Amygdala, And Entorhinal Cortex, Andrew M. Meier, Quanxin Wang, Weiqing Ji, Jehan Ganachaud, Andreas Burkhalter

Open Access Publications

The postrhinal area (POR) is a known center for integrating spatial with nonspatial visual information and a possible hub for influencing landmark navigation by affective input from the amygdala. This may involve specific circuits within muscarinic acetylcholine receptor 2 (M2)-positive (M2

Is The Intestine A Portal Of Entry For The Serious Covid-19 Complications Of Endotoxemia And Thrombosis?, David H. Alpers

Open Access Publications

No abstract provided.

New Cohort Of Patients With Cednik Syndrome Expands The Phenotypic And Genotypic Spectra, Annelise Y. Mah-Som, Cristina Skrypnyk, Andrea Guerin, Raafat Hammad Seroor Jadah, Vinayak Nivrutti Vardhan, Robert C. Mckinstry, Marwan S. Shinawi

Open Access Publications

No abstract provided.

A Clinician's Guide For Navigating The World Of Attention Deficit Hyperactivity Disorder Medications, Gregory W Mattingly, Joel Young

Open Access Publications

No abstract provided.

Telomere Erosion In Human Pluripotent Stem Cells Leads To Atr-Mediated Mitotic Catastrophe, Alexandre T. Vessoni, Tianpeng Zhang, Annabel Quinet, Ho-Chang Jeong, Michael Munroe, Matthew Wood, Enzo Tedone, Alessandro Vindigni, Jerry W. Shay, Roger A. Greenberg, Luis F.Z. Batista

Open Access Publications

It is well established that short telomeres activate an ATM-driven DNA damage response that leads to senescence in terminally differentiated cells. However, technical limitations have hampered our understanding of how telomere shortening is signaled in human stem cells. Here, we show that telomere attrition induces ssDNA accumulation (G-strand) at telomeres in human pluripotent stem cells (hPSCs), but not in their differentiated progeny. This led to a unique role for ATR in the response of hPSCs to telomere shortening that culminated in an extended S/G2 cell cycle phase and a longer period of mitosis, which was associated with aneuploidy and mitotic …

Aging-Associated Deficit In Ccr7 Is Linked To Worsened Glymphatic Function, Cognition, Neuroinflammation, And Β-Amyloid Pathology, Sandro Da Mesquita, Jasmin Herz, Morgan Wall, Taitea Dykstra, Kalil Alves De Lima, Geoffrey T Norris, Nisha Dabhi, Tatiana Kennedy, Wendy Baker, Jonathan Kipnis

Open Access Publications

Aging leads to a progressive deterioration of meningeal lymphatics and peripheral immunity, which may accelerate cognitive decline. We hypothesized that an age-related reduction in C-C chemokine receptor type 7 (CCR7)-dependent egress of immune cells through the lymphatic vasculature mediates some aspects of brain aging and potentially exacerbates cognitive decline and Alzheimer's disease-like brain β-amyloid (Aβ) pathology. We report a reduction in CCR7 expression by meningeal T cells in old mice that is linked to increased effector and regulatory T cells. Hematopoietic CCR7 deficiency mimicked the aging-associated changes in meningeal T cells and led to reduced glymphatic influx and cognitive impairment. …

Trem2 Is A Receptor For Non-Glycosylated Mycolic Acids Of Mycobacteria That Limits Antimycobacterial Macrophage Activation, Ei'ichi Iizasa, Yasushi Chuma, Takayuki Uematsu, Mio Kubota, Hiroaki Kawaguchi, Masayuki Umemura, Kenji Toyonaga, Hideyasu Kiyohara, Ikuya Yano, Marco Colonna, Masahiko Sugita, Goro Matsuzaki, Sho Yamasaki, Hiroki Yoshida, Hiromitsu Hara

Open Access Publications

No abstract provided.

All Epidural Needle Bevels Are Not The Same, Amber C. Benhardt, Bhavani Shankar Kodali

Open Access Publications

No abstract provided.

Endothelial Ether Lipids Link The Vasculature To Blood Pressure, Behavior, And Neurodegeneration, Larry D. Spears, Sangeeta Adak, Guifang Dong, Xiaochao Wei, George Spyropoulos, Qiang Zhang, Li Yin, Chu Feng, Donghua Hu, Irfan J. Lodhi, Fong-Fu Hsu, Rithwick Rajagopal, Kevin K. Noguchi, Carmen M. Halabi, Lindsey Brier, Annie R. Bice, Brian V. Lananna, Erik S. Musiek, Oshri Avraham, Valeria Cavalli, Jerrah K. Holth, David M. Holtzman, David F. Wozniak, Joseph P. Culver, Clay F. Semenkovich

Open Access Publications

No abstract provided.

Effects Of Dietary Fatty Acid Composition On Lipid Metabolism And Body Fat Accumulation In Ovariectomized Rats, Jhih-Han Yeh, Yu-Tang Tung, Yu-Sheng Yeh, Yi-Wen Chien

Open Access Publications

BACKGROUND: Obesity is a state of excess energy storage resulting in body fat accumulation, and postmenopausal obesity is a rising issue. In this study using ovariectomized (OVX) rats, we mimicked low estrogen levels in a postmenopausal state in order to investigate the effects of different amounts and types of dietary fatty acids on body fat accumulation and body lipid metabolism.

METHODS: At 9 weeks of age, rats (

RESULTS: After OVX, compared to the S group, the C group showed significantly higher body weight, and insulin and leptin levels. Compared to the C group, the H group had lower hepatic …

Acitretin Mitigates Uroporphyrin‑Induced Bone Defects In Congenital Erythropoietic Porphyria Models, Juliana Bragazzi Cunha, Jared S. Elenbaas, Dhiman Maitra, Ning Kuo, Rodrigo Azuero-Dajud, Allison C. Ferguson, Megan S. Griffin, Stephen I. Lentz, Jordan A. Shavit, M. Bishr Omary

Open Access Publications

No abstract provided.

Informative Regions In Viral Genomes, Jaime Leonardo Moreno-Gallego, Alejandro Reyes

Open Access Publications

Viruses, far from being just parasites affecting hosts' fitness, are major players in any microbial ecosystem. In spite of their broad abundance, viruses, in particular bacteriophages, remain largely unknown since only about 20% of sequences obtained from viral community DNA surveys could be annotated by comparison with public databases. In order to shed some light into this genetic dark matter we expanded the search of orthologous groups as potential markers to viral taxonomy from bacteriophages and included eukaryotic viruses, establishing a set of 31,150 ViPhOGs (Eukaryotic Viruses and Phages Orthologous Groups). To do this, we examine the non-redundant viral diversity …

Acceleration Of Small Intestine Development And Remodeling Of The Microbiome Following Hyaluronan 35 Kda Treatment In Neonatal Mice, Hala Chaaban, Kathryn Burge, Jeffrey Eckert, Majoi Trammell, David Dyer, Ravi S Keshari, Robert Silasi, Girija Regmi, Cristina Lupu, Misty Good, Steven J Mcelroy, Florea Lupu

Open Access Publications

The beneficial effects of human milk suppressing the development of intestinal pathologies such as necrotizing enterocolitis in preterm infants are widely known. Human milk (HM) is rich in a multitude of bioactive factors that play major roles in promoting postnatal maturation, differentiation, and the development of the microbiome. Previous studies showed that HM is rich in hyaluronan (HA) especially in colostrum and early milk. This study aims to determine the role of HA 35 KDa, a HM HA mimic, on intestinal proliferation, differentiation, and the development of the intestinal microbiome. We show that oral HA 35 KDa supplementation for 7 …

Prospective Spect-Ct Organ Dosimetry-Driven Radiation-Absorbed Dose Escalation Using The In-111 (111in)/Yttrium 90 (90y) Ibritumomab Tiuxetan (Zevalin ®) Theranostic Pair In Patients With Lymphoma At Myeloablative Dose Levels, Richard L Wahl, Eric C Frey, Heather A Jacene, Brad S Kahl, Steven Piantadosi, Jesus A Bianco, Richard J Hammes, Miah Jung, Wayne Kasecamp, Bin He, George Sgouros, Ian W Flinn, Lode J Swinnen

Open Access Publications

PURPOSE: We prospectively evaluated the feasibility of SPECT-CT/planar organ dosimetry-based radiation dose escalation radioimmunotherapy in patients with recurrent non-Hodgkin's lymphoma using the theranostic pair of

METHODS: 24 patients with CD20-positive relapsed or refractory rituximab-sensitive, low-grade, mantle cell, or diffuse large-cell NHL, with normal organ function, platelet counts > 75,000/mm

RESULTS: Patient-specific hybrid SPECT/CT + planar organ dosimetry was feasible in all 18 cases and used to determine the patient-specific therapeutic dose and guide dose escalation (26.8 ± 7.3 MBq/kg (mean), 26.3 MBq/kg (median) of

CONCLUSIONS: Patient-specific outpatient

Single Cell Transcriptional And Chromatin Accessibility Profiling Redefine Cellular Heterogeneity In The Adult Human Kidney, Yoshiharu Muto, Parker C. Wilson, Nicolas Ledru, Haojia Wu, Henrik Dimke, Sushrut S. Waikar, Benjamin D. Humphreys

Open Access Publications

No abstract provided.