Digital Commons Network^™

Photopigments As Descriptors Of Phytoplankton Assemblages For Biotic Assessment Of Illinois Lakes And Reservoirs: An Hplc Aided Analysis, Robert J. Krenz Iii

Masters Theses

The Illinois Environmental Protection Agency (IEPA) and U.S. Environmental Protection Agency (USEP A) identify needs to monitor aquatic resources by quantitative means using biocriteria in conjunction with routine environmental assessment to identify sources of impairment, and determine the effectiveness of management protocols (IEP A 2007; USEPA 2007). Though bioassessment protocols have been implemented within lotic systems and lakes of natural origin, investigation of their application to functionally unique reservoir habitat, which dominates the Midwest, has been limited. This study .evaluated the utility of photopigment profiles, attained using high-performance liquid chromatography (HPLC), for development ofbioassessment protocols for Illinois lakes and reservoirs. …

Disparity Between Boys' And Girls' Academic Achievement, Laura M. Harris

Masters Theses

The purpose of this study was to identify the factors that contribute to the disparity between the academic achievement level of boys and girls in the school setting. Current literature indicates that while boys have historically outperformed girls in the area of math, girls have all but closed this achievement gap.

One hundred and twenty-four fourth and fifth grade students (71 girls, 53 boys, predominantly Caucasian) from a school in the Midwest region of the United States participated in the study. Gender and living with a male role model were found to have no relationship with academic achievement. These results …

Familial Factors And Attachment Styles Of Adult Children Of Alcoholics, Tiffany Marie Konz

Masters Theses

Substance abuse is a prevalent issue that has far-reaching implications for not only the abuser but also the family. Research on parental alcoholism and its effects on offspring have developed over the last several years. Following the lead of other researchers, the present study aimed to examine the relationship between parental alcoholism and attachment styles in adult children of alcoholics (ACOAs) and possible mediating or moderating effects of familial factors. A sample of 84 college students completed a series of measures on parental alcoholism, family satisfaction, family cohesion, parent-child attachment, and psychological and physical maltreatment. Participants were divided into two …

Analysis Of Lesion Border Segmentation Using Watershed Algorithm, Hanzheng Wang

Masters Theses

"Automatic lesion segmentation is an important part of computer-based skin cancer detection. A watershed algorithm was introduced and tested on benign and melanoma images. The average of three dermatologists' manually drawn borders was compared as the benchmark. Hair removing, black border removing and vignette removing methods were introduced in preprocessing steps. A new lesion ratio estimate was added to the merging method, which was determined by the outer bounding box ratio. In postprocessing, small blob removing and border smoothing using a peninsula removing method as well as a second order B-Spline smoothing method were included. A novel threshold was developed …

Automated Offspring Sizing In Evolutionary Algorithms, André Chidi Nwamba

Masters Theses

"Evolutionary Algorithms (EAs) are a class of algorithms inspired by biological evolution. EAs are applicable to a wide range of problems; however, there are a number of parameters to set in order to use an EA. The performance of an EA is extremely sensitive to these parameter values; setting these parameters often requires expert knowledge of EAs. This prevents EAs from being more widely adopted by nonexperts. Parameter control, the automation of dynamic parameter value selection, has the potential to not only alleviate the burden of parameter tuning, but also to improve performance of EAs on a variety of problem …

Time-Weighted Average Solid-Phase Microextraction (Twa-Spme) For In-Planta Detection Of Chlorinated Solvents, Emily Moore Sheehan

Masters Theses

"Recent interest in the use of phyto-mapping for plume delineation at contaminated sites has promoted a need for new and innovative sampling techniques. Solid-phase micro-extraction (SPME) methods have been developed as a chemical analysis tool offering fast, simple, non-invasive sampling without the use of solvents. In this study SPME devices were tested for applicability for in-planta detection of chlorinated solvents. To evaluate the use of SPME for VOCs in-planta a number of integrated studies were undertaken. Uptake profiles were developed for nine chlorinated solvents in a liquid polydimethylsiloxane (PDMS) matrix with 100 µm Carboxene SPME fibers. Timeweighted average (TWA) sampling …

Electricity Generation And Ethanol Production Using Iron-Reducing, Haloalkaliphilic Bacteria, Varun Paul

Masters Theses

"Microbial life in extreme environments has been studied primarily for their metabolic activities. Very few commercial or industrial applications have been known from these systems. In this study, the metabolic pathways and properties of bacteria from a haloalkaliphilic environment of Soap Lake, Washington were employed in two research applications related to energy production. In the first study, the bacterial cultures that were known to reduce iron (III) were used in a Microbial Fuel Cell (MFC) reactor. Iron reducing bacteria have shown to shunt their electrons on to the surface of a carbon electrode of an MFC, and the electrons can …

Exploring The Use Of A Commercial Game Engine For The Development Of Educational Software, Hussain Alafaireet

Masters Theses

"Three-dimensional visualization and simulation environments are becoming increasingly important in both industrial and educational environments. However, developing these simulations can be a difficult and expensive task. Herein we explore the use of a commercial game engine to develop a 3-D, interactive computer game designed to teach players about the anatomy of a frog. The goal of this project is threefold: 1) to develop high-fidelity virtual reality educational software with a limited budget and limited personnel, 2) to define a software development process by which similar simulations can be developed, and 3) to explore the benefits that these simulations provide to …

Development And Evaluation Of An Effective Process For The Recovery Of Oil And Detoxification Of Meal From Jatropha Curcas, Sarthak Gaur

Masters Theses

"There has been significant research on biofuels to ensure national security and economic stability of the US. Jatropha curcas, a hardy plant, holds tremendous potential as a biodiesel source and cattle feed-stock largely due to its high triglyceride and crude protein content. However, its viable economic utilization is limited due to the presence of important toxic compounds, phorbol esters. A novel and efficient process has been developed that obtains high yields of jatropha oil and detoxifies the defatted (oil free) jatropha meal. Principles of solid-liquid extraction were utilized to detoxify the meal and then liquid chromatography was employed to analyze …

Fault Detection And Prediction With Application To Rotating Machinery, Gary R. Halligan

Masters Theses

"In this thesis, the detection and prediction of faults in rotating machinery is undertaken and presented in two papers. In the first paper, Principal Component Analysis (PCA), a well known data-driven dimension reduction technique, is applied to data for normal operation and four fault conditions from a one-half horsepower centrifugal water pump. Fault isolation in this scheme is done by observing the location of the data points in the Principal Component domain, and the time to failure (TTF) is calculated by applying statistical regression on the resulting PC scores. The application of the proposed scheme demonstrated that PCA was able …

Nona-Arginine Peptides Facilitate Cellular Entry Of Semiconductor Nanocrystals: Mechanisms Of Uptake, Yi Xu

Masters Theses

"Luminescent semiconductor quantum dots (QDs) have recently been used for delivering and monitoring biomolecules, such as drugs and proteins. However, QDs alone have a very low efficiency of transport across the plasma membrane. In order to increase the efficiency of QD delivery, synthetic nona-arginine (sR9) was used, a cell penetrating peptide, to facilitate uptake. Data demonstrated that sR9 could significantly increase the cellular uptake of QDs by noncovalent binding between QDs and sR9. Furthermore, the mechanisms of QD/sR9 cellular internalization were investigated. Low temperature and metabolic inhibitors markedly inhibited the uptake of QD/sR9, indicating that internalization is an energy-dependent process. …

Oxidative Stress, Calcium Homeostasis, And Altered Gene Expression In Human Lung Epithelial Cells Exposed To Zno Nanoparticles, Chuan-Chin Huang

Masters Theses

"The influence of 20 nm ZnO nanoparticles on oxidative stress, intracellular calcium homeostasis, and gene expression was studied in human bronchial epithelial cells (BEAS-2B). ZnO caused a concentration- and time-dependent cytotoxicity while elevating oxidative stress and causing membrane damage (cellular LDH release). There was a remarkably steep relationship between concentration and toxicity at concentrations from 5 to 10 μg/ml. Exposure to ZnO increased intracellular calcium levels in a concentration- and time-dependent manner. Treatment with the antioxidant N-acetylcysteine prevented cell loss and diminished the increase in intracellular calcium concentration, suggesting oxidative stress mediated cytotoxicity. Exposure to a sublethal concentration of ZnO …

Environmental Geochemistry Of Metal Contaminated Sediments From The Big River System Of Southeastern Missouri, Helen Carrie Bender

Masters Theses

"Geochemical analysis of stream sediments from the Big River Watershed of southeastern Missouri indicates that they contain elevated concentrations of contaminant metals such as Pb. Zn, Cu. Co, Ni and Cd. The elevated concentrations are derived from natural exposures of metal enriched strata and the numerous mine tailings piles and water seeps created as a result of about 300 years of lead-zinc mining in the “Old Lead Belt”. Galena (PbS), sphalerite (ZnS), and pyrite (FeS₂) are the primary sulfides found in the tailings piles and Scanning Electron Microscopy analysis of individual metallic sediment particles collected at intervals downstream …

In Vitro Evaluation Of Bioactive Glass Scaffolds And Modified Bioactive Glasses With An Osteogenic Cell Line, Vernon C. Modglin

Masters Theses

"This investigation consisted of two parts focused on bioactive glasses and scaffolds for in vitro growth of bone cells for tissue engineering and bone repair. The first part involved evaluation of the ability of two new types of porous 13-93 bioactive glass scaffolds to support the growth and differentiation of mouse MLO-AS cells, an established osteogenic cell line used as a model of developing bone tissue. The two scaffold types tested included 13-93 glass fiber and trabecular-like scaffolds seeded with MLO-AS cells and cultured for intervals of 2 to 12 days. Collectively, in vitro results indicate that the 13-93 glass …

An Evolutionary Algorithm Approach To Actuated Signal Timing Optimization Under Lane-By-Lane Detection Condition, Eric G. Strack

Masters Theses

"Motivated by the need to reduce congestion on the nation's roadway network, this research seeks to determine when a new detection scheme at actuated signalized intersections may help. This new detection scheme is called lane-by-lane detection. It "looks" at traffic in all lanes individually to determine the traffic volume on the roadway. Specifically, the problems addressed are investigating alternate signal timings and investigating the traffic volume's effect on performance of the detection scheme. For the alternate signal timings, an Evolutionary Algorithm (EA) was employed to identify the optimal signal timing. This algorithm creates signal timings and tests them in the …

Where We Can't Follow: A Collection Of Short Stories, Megan Holt

Masters Theses

This humorous collection of short stories revolves around the lives of the patrons and employees who frequent the bars. When men and women come together to congregate over drinks, their conversations turn towards every topic except the important aspects of their various relationships. As underlying conflicts rise to the surface, and the situations become more comical, the characters are faced with a brief epiphany about the true nature of their personalities. Rather than becoming inspired by these moments of clarity, these men and women resist the urge to change. The liquor continues to flow, inhibitions flounder, and these characters hurtle …

Dynamics Of Aggregation Formation In Japanese Beetles, Popillia Japonica, Katelyn A. Kowles

Masters Theses

For most insect aggregations to form, they need to be started by an initial individual (the pioneer) andjoined by later individuals (the joiners). Pioneers and joiners may differ with regard to characteristics such as sex and body size. We carried out three field experiments to examine the characteristics of Japanese beetles, Popillia japonica, pioneering and joining aggregations on host plants. Individual beetles were captured as they arrived on uninhabited grape plants, as well as plants designed to simulate aggregations with model beetles and feeding damage. For all experiments and treatments, the beetles arriving were significantly female-biased, with pioneer females having …

Cloning, Overexpression, Purification And Characterization Of Gcp Ii Homologous Metalloprotein From Caulobacter Crescentus, Dilani Nilushika Gigiriwala Gamage

Masters Theses

A novel zinc metalloprotease gene isolated from Caulobacter crescentus (CCM) was cloned and overexpressed in Escherichia coli. A protease database search using MEROPS predicted this protein is a homolog of Glutamate Carboxypeptidase II (GCP II), a zinc metalloprotease found in humans and many other organisms. GCP II is considered as a suitable target for treating stroke, neurological disorders and a biomarker for prostate cancer. Chromatographic purification of CCM produced pure protein in milligram quantities. Molecular weight detern1ination using MALDI-TOF showed 51,582 Da and the extinction coefficient was found to be 33,007 M"1cm·1. Metal analysis and substrate profiling showed neither sufficient …

Stability Of Heme Proteins: Cytochrome C And Horseradish Peroxidase, Mini Gupta

Masters Theses

A heme protein is a metalloprotein containing the heme prosthetic group. This prosthetic group is responsible for the wide range of biological functions found in heme proteins, from oxygen transport to electron transport. Moreover, heme proteins show a fascinating range of folding architectures, leading to their interest in research on structure-function relationships. The objective of this research in bioinorganic chemistry is to study the structural stability of two heme proteins, iron cytochrome c (Fecyt) and horseradish peroxidase (HRP). Absorbance spectroscopy is used to monitor the structural changes in the protein environment around the heme prosthetic group.

With just a change …

Electrochemical And Spectroscopic Characterization Of A Polypyridyl Ru(Ii) Complex Containing A Fused Alloxazine, Waynie Wilson Olaprath

Masters Theses

Phenanthroline pteridinedione (pptd) is an alloxazine derivative capable of 2e/ 2H+ transfer reactions. Coordination of pptd to a Ru(II) metal center allows for the synthesis of water-soluble metal complexes capable of being reduced by as many as 6 Hatoms making them candidates to be electrochemical mediators. This thesis describes the synthesis and characterization of pptd and [Ru(bpy)2(pptd)](PF6)2(b py = 2,2-bipyridine). The dipyrido[3,2-a: 1' ,2' -c] phenazine (d ppz) and [Ru(bpy)2(d ppz) ](PF6)2 are also synthesized and studied in order to distinguish the properties of the free and coordinated pteridinedione-based pptd ligand from the well-characterized phenazine based ligand.

Detailed cyclic voltammetry, …

Ecology And Impacts Of Lianas In Regenerating Forests, Laura Marie Ladwig

Masters Theses

Although they are important components of forest communities, the general ecology of temperate lianas, especially during forest regeneration, is largely unknown. The dependence of lianas on other plants for physical support makes them a potentially important driver of community dynamics. I examined current and past vegetation data from an old-field succession study in the Piedmont region of New Jersey, USA to determine the dynamics of liana expansion and their role in forest regeneration. Fifty years of vegetation data were utilized to determine community controls and population dynamics oflianas during expansion. Five lianas, Celastrus orbiculatus, Lonicera japonica, Parthenocissus quinquefolia, Toxicodendron radicans, …

Aging In The Mammalian Olfactory System, Stephen Clark

Masters Theses

The purpose of this investigation was to investigate genetic and age~related differences in the mammalian olfactory system. The molecule of interest is apolipoprotein E (apoE), a component oflipoprotein molecules which functions in the transport of cell membrane components (triglycerides, lipids, and cholesterol). Specifically in the nervous system, apoE is synthesized by glial cells that function in recycling dead nervous tissue components for delivery to growing or dividing neurons. ApoE is present in three protein isofon11s: apoE2, which has been associated with type III hyperlipoproteinemia; apoE3, rhe normal human isoforrn; and apoE4, which has been associated with Alzheimer's disease (AD). The …

Interaction Of Apoe With Estrogen In The Olfactory System During Nerve Maintenance And Recovery, Michael M. Tonsor Ii

Masters Theses

Estrogen replacement profoundly aids in nerve regeneration after injury in adult mammalian models. Estrogen replacement also protects against olfactory dysfunction in mammals. The mechanism underly1ng estrogen's neuroprotective effects remains ambiguous. My goal is to investigate and better clarify if apolipoprotein E (apoE ), a lipid transporting protein, is a critical intermediary to estrogen's effects on nerve maintenance and regeneration.

My hypothesis is that apoE is a mediator of estrogen's beneficial effects. This hypothesis is based on the following observations from previous studies. First, olfactory nerve regeneration was delayed in apoE-gene knockout (KO) mice as compared to wild type (WT) mice. …

Fragmentation Effects On Fitness In Five Common Prairie Species, Lydia Miramontes Loyd

Masters Theses

Midwest tallgrass prairies have suffered extensive habitat loss and as a result persist as isolated and highly fragmented remnants. In such small, isolated remnants, genetic drift and inbreeding depression can lead to a loss of genetic variation over time. Reduced genetic variation is often associated with reduced levels of growth, survival, and reproduction as well as increased susceptibility to stress. This study examined fitness traits of five common prairie species to determine whether plants in small fragmented remnants had reduced fitness as compared to those in a larger remnant. Five prairie species were chosen for this study based on their …

Nest Success, Nesting Habitat, & Nestling Feeding Rates Of Red-Headed Woodpeckers In East-Central Illinois, Nathan Cole Hudson

Masters Theses

The Red-headed Woodpecker (Melanerpes erythrocephalus) is a classic oak savanna species that has suffered significant population declines caused, in part, by extensive loss of savanna habitat and agricultural development. Despite consistent data that reveal a dramatic decline throughout much of their range, few studies have examined other possible explanations. In an effort to determine potential causes for their continued decline (as well as add to existing knowledge of nesting biology), we examined nest success and nesting micro-habitat features. We compared micro-habitat variables at: 1) golf and non-golf course sites, 2) successful and failed nest sites, and 3) nest and random …

Attention Deficit Hyperactivity Disorder In Elementary School Children In A Rural Illinois School District, Amy M. Miller

Masters Theses

Attention Deficit Hyperactivity Disorder (ADHD), one of the most commonly diagnosed childhood disorders, adversely affects children's academic, social, and emotional functioning. There were three purposes for this study. First, this study attempted to determine the number of students diagnosed with ADHD in the three elementary schools of a rural Midwestern school district as reported by parents. Secondly, the study attempted to discover discrepant practices in the methods of diagnosis and treatment ofADHD for students from a lower socioeconomic status than for those from the middle or upper class as reported by the parents. Thirdly, this study assessed teachers' knowledge and …

Analysis Of Transcription Factor Promoter Binding In Rat Hepatoma/Fibroblast Hybrids, Jordan C. Angle

Masters Theses

When hepatomas (liver cancer cells) are fused with fibroblasts (undifferentiated body cells), there is complete extinguishing of all tissue-specific gene expression. Once it was discovered that many hybrid cell lines do not express needed transcription factors for liver-specific gene transcription, the factors were reintroduced and forced to express through the use of plasmids. Even if the two primary transcription factors were present in the system, downstream tissue-specific gene expression was not rescued. To examine potential mechanisms for the repression of liver-specific gene expression in hepatoma x fibroblast hybrid cells, chromatin immunoprecipitation (ChlP) was used. This procedure allows the liver-specific gene …

Long-Term Assessment Of Predatory Fish Removal On A Pond-Breeding Amphibian Community In Central Illinois, Lee M. Gross

Masters Theses

Habitat loss and fragmentation are important contributors to population declines in many species, so maintaining suitable habitat has become a priority for wildlife management. Removal of introduced fish can help restore suitable habitats for amphibian populations, although long-term assessments of such removals are lacking at the community level. In 2001, drift fences and pitfall traps were constructed around the majority of four ponds to monitor an amphibian community in a nature preserve in central Illinois. Rotenone™ was applied to ponds containing introduced fish in 2001, and again, in 2003. I collected data from 2005-2007 to determine the long-term impacts of …

Habitat Selection And Niche Partitioning Of Fox And (Sciurus Niger) And Gray (Sciurus Carolinensis) Squirrels In Charleston, Il, Katelyn Marti

Masters Theses

Two species of tree squirrels inhabit Charleston, IL; fox squirrels (Sciurus niger) and gray squirrels (Sciurus caro/inensis). Although these species are sympatric throughout much of the eastern U.S., urban settings are usually occupied by one or the other. In the few localities where they co-occur, little is known about how they partition habitat. Therefore, the objectives of my study were to determine: (1) the relative abundance of each species, (2) whether habitat use differs between species, (3) if habitat use shifts with season, time of day, domestic predator abundance, or mast availability, and (3) observe interspecific and intraspecific interactions. The …

Survival Strategies Of The Endangered Physaria Ludoviciana (Silvery Bladderpod; Brassicaceae), Marissa Catherine Jernegan Grant

Masters Theses

Physaria ludoviciana (Nuttal) O'Kane and Al-Shehbaz is an endangered species in Illinois and Minnesota sand prairies, but has many individual populations in western states. Areas where P. ludoviciana occurs have high summer temperatures, a sandy soil with low water holding capacity and high drainage, the frequent disturbance of blowing sand and little canopy vegetation, giving it a full sun environment. This habitat is where few other plants are able to establish, providing P. ludoviciana with little competition. Physaria ludoviciana exists in a niche where it is adapted and able to establish and persist. The focus of this study was to …