Digital Commons Network^™

Tumor Immunology: Understanding The Immune System And Cancer To Enhance The Efficacy Of Cancer Immunotherapies, Natasha Malibu Lenart

Master's Theses

Tumor cells are notorious for their ability to escape immune surveillance, but developments in the understanding of the tumor microenvironment and how the immune system can be re-activated in tumors have had significant clinical impact. Commercially available and experimental methods such as adoptive cellular therapy, cytokine stimulation, and immune checkpoint blockade are promising immunotherapies for a variety of cancers, including solid tumors and hematological malignancies. However, induction of persistent, long-term anti-tumor immunity after initial treatment is infamously difficult. as a result, scientists are searching for new approaches to improve established immunotherapies. by employing combination treatments or enhancing the functionality of …

The Optimum Design Of A Vacuum-Compatible Manipulator To Calibrate Space Based Ultraviolet Imagers, Jason L. Grillo

Master's Theses

Recent discoveries in geospace science have necessitated the design of compact UV imaging instruments to make space-based observations from multiple vantage points. The miniaturized ultraviolet imager (MUVI) instrument from the Space Sciences Laboratory (SSL) at UC Berkeley is under development to facilitate such discoveries on a wider scale. This thesis documents the design, integration, and characterization of a vacuum compatible manipulator to calibrate the MUVI instrument inside the UV thermal vacuum chamber at SSL. Precision linear and rotation stages were implemented with custom mounting plates to achieve four degrees of freedom. Optical components were installed to imitate the MUVI instrument …

Red Blood Cell Biomechanics In Myalgic Encephalomyelitis/Chronic Fatigue Syndrome, Arun Kumar

Master's Theses

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is characterized by severe fatigue, impaired cognition, and discomfort. Unfortunately, the etiology and pathogenesis of the disease are not well understood, and there are no diagnostic biomarkers. Early studies have shown that the red blood cells (RBC) in ME/CFS patients have poor filterability. This finding has been implicated in impaired microvascular oxygenation, which may contribute to post exertional malaise and profound fatigue. We hypothesized that RBC biomechanics in patients with ME/CFS are significantly different from those in healthy controls. To test this hypothesis, we use a microfluidic device with microchannels that are narrower than the …

Optimizing Ferroelectric Nanowire Fet For Sub-60mv/Decade Sub-Threshold Slope, Abhishek Kartik Raol

Master's Theses

Limitations in traditional scaling methods require novel devices to increase computational density without scaling down device size. Ferroelectric materials’ negative capacitance property reduces the subthreshold slope beyond the previous theoretical ideal of 60 mV/decade and improves computational density without reducing transistor size. The research herein characterizes fields in isolated ferroelectric material, ferroelectric-oxide interfaces, and ferroelectric material integrated in the gate-stack of a ferroelectric nanowire-FET (FeNW) to achieve sub 60 mV/decade subthreshold slope. The research attains sub 60 mV/decade subthreshold slope across multiple FeNW configurations, and subsequently provides a ferroelectric-to-oxide capacitance, ratio-based design method to approaching sub-60 mV/decade subthreshold slope and …

Prediction Of Novel Antibiofilm Peptides From Diverse Habitats Using Machine Learning, Bipasa Bose

Master's Theses

Multidrug resistant bacteria often lead to biofilm formation. Biofilm is a colonizedform of pathogens (fungi, bacteria) attached to surfaces like animal or plant tissues, medical devices like catheters, and artificial heart valves. Biofilm formation prolongs the survival of microorganisms in an adaptive environment, leading to the spread of infection in different organs and causing a high morbidity rate. Given the rise of chronic infection and antibiotic resistance due to biofilm, it is essential to find an alternative solution to control biofilm infections. Antibiofilm peptides can interact with these biofilm-creating pathogens to inhibit growth, virulence, and biofilm formation. We hypothesized that …

Spatial And Temporal Effects Of Dry Air On Tropical Cyclone Intensity And Tracks Over The Eastern Atlantic Ocean Basin, Amy Ip

Master's Theses

This study aims to understand how often dry air occurs or co-exists with a tropical cyclone (TC) and to what degree dry air will affect the way the TC proceeds. Four tropical cyclones of 2015 were chosen: Hurricane Fred, Hurricane Danny, Tropical Storm (TS) Grace and Tropical Depression (TD) 9 as their formation and tracks were located within the eastern Atlantic Ocean region. Relative humidity (RH) from MERRA-2 and NCEP-FNL datasets were compared within 3 days of the highest storm status and looking forward 3 days on the storm path. Results at 700 hPa suggested that dry air (≤30% RH) …

Processing And Characterization Of Ceria-Zirconia Containing Graphene Quantum Dots, Andrew Dina

Master's Theses

Zirconia-based nanoparticles have been used in various applications, such as catalyst, filters, oxygen storage, and sensors. Among many synthesis methods to produce zirconia (ZrO2) nanoparticles, aqueous sodium borohydride (NaBH4) precipitation has shown to be simple and capable of large-scale production. In this work, the NaBH4 precipitation of ZrO2 was extended to include ceria (CeO2) and graphene quantum dots (GQDs). The resulting nanocomposites were characterized by thermal, crystallographic, compositional, and electron microscopy analysis techniques. The presence of CeO2 lowered the amorphous to tetragonal phase transformation temperature by ~40 ℃. The presence of GQDs did not affect the amorphous to tetragonal transformation …

A Study Of Inactive Enzyme-Homologues: The Biochemical And Biological Function Of Ecm14 In Saccharomyces Cerevisiae, Robert Christian Mcdonald

Master's Theses

Like most major enzyme families, the M14 family of metallocarboxypeptidases (MCPs) contains several pseudoenzymes predicted to lack enzyme activity and with unknown molecular function. The genome of the yeast Saccharomyces cerevisiae encodes only one member of the M14 MCP family, a pseudoenzyme named Ecm14 proposed to function in the extracellular matrix. Ecm14 is found throughout ascomycete fungi, with a group of related pseudoenzymes found in basidiomycetes. Although the prodomain of Ecm14 can be cleaved in vivo and in vitro by endopeptidases, suggesting an activation mechanism, no activity has been detected using standard carboxypeptidase substrates.

In order to examine the function …

The Casemate Wall System Of Khirbat Safra, Elizabeth Anne Emswiler

Master's Theses

Problem

A casemate wall system was found at Khirbat Safra in 2018 and 2019, which has been dated to early Iron Age I. However, no comprehensive study of Iron Age I casemate wall systems in Transjordan has been conducted. Therefore, this thesis will present and compare the findings from Kh. Safra and four other sites in Transjordan which have casemate wall systems dating to Iron Age I.

Method

A short study on the history of fortifications, casemate wall systems, and siege warfare was conducted for this thesis. The ruins from Fields A, B, C, and D at Kh. Safra were …

Human Path Prediction Using Auto Encoder Lstms And Single Temporal Encoders, Hayden Hudgins

Master's Theses

Due to automation, the world is changing at a rapid pace. Autonomous agents have become more common over the last several years and, as a result, have created a need for improved software to back them up. The most important aspect of this greater software is path prediction, as robots need to be able to decide where to move in the future. In order to accomplish this, a robot must know how to avoid humans, putting frame prediction at the core of many modern day solutions. A popular way to solve this complex problem of frame prediction is Auto Encoder …

Predicting Drug Misuse Status Using Machine Learning On Electronic Health Records, Robert Arnold Kania

Master's Theses

Substance misuse is a major problem in the world. in 2014, as many as 52,404 deaths in the US were caused by drug overdoses. in 2001, the monetary cost of drug misuse has been estimated to be 414 billion dollars. in this work, we explore the use of different machine learning algorithms in the prediction of cocaine misuse using structured and unstructured data found in electronic health records. These records contain various attributes that can help with this prediction, including but not limited to chart text data, previous diagnoses of certain diseases and information about the area the patient lives …

Wayfinder Application For Autistic Occupational Assistance, Nathaniel Edward Hishon

Master's Theses

Employment among autistic individuals is an area of noted difficulty, with an employment rate well below the general population [1]. Several barriers attributed to autistic unemployment, including difficulties communicating with employers and social interactions with coworkers, obsessive adherence to routine, and trouble organizing and completing workplace tasks, are also attributed to challenges in maintaining employment [2]. Several studies have concluded that long-term employment support is necessary to acquire and maintain autistic employment [3]. The noted benefit of intensive job training, such as access to job coaches, indicates the need for further support to help autistic individuals complete workplace tasks and …

Using Blockchain Technology For The Organ Procurement And Transplant Network, Utsav Jain

Master's Theses

The organ donation system in the United States is centralized and difficult to audit by the general public. This centralized approach may lead to data integrity issues in the future. The Organ Procurement and Transplant Network (OPTN) was built and maintained by a non-governmental organization called the United Network for Organ Sharing (UNOS) under its proprietary UNet(SM) umbrella platform. This platform is made up of proprietary closed source software and does not provide the general public easy access to the organ transplant data for auditing. This study investigates the feasibility, challenges, and advantages of a blockchain-based OPTN.

A prototype of …

The Top-Down Influences Of Characteristic Sounds On Visual Search Performance In Realistic Scenes, Ghazaleh Mahzouni

Master's Theses

The purpose of this experiment was to investigate whether meaningful sounds can facilitate visual search performance in the context of realistic scenes. It also aimed to determine whether the stimulus onset asynchrony (SOA) of sound and picture is a significant factor in enhancing performance. A 3 X 4 X 2 within subject design was used with independent factors sound congruency (congruent, incongruent and white noise), SOA (-1000, -500, 0, 300 ms), and target presence (present and absent). Participants were 55 (34 female and 21 male) college aged students at San Jose State University. On each trial participants were presented with …

High-Throughput Screening Of A Metagenomic Library Using A Novel E. Coli Chip For Discovery Of Antibiotic Resistance Genes, Richard Paul Manarin Tiongco

Master's Theses

The growing prevalence of antibiotic resistance is one of the greatest challenges facing humankind today. The Centers for Disease Control and Prevention (CDC) have estimated that about 2 million people in the US each year develop an infection caused by antibiotic-resistant bacteria, and about 23,000 people die. To combat this growing crisis, efforts geared toward the discovery of novel compounds, such as antimicrobial peptides (AMPs), have spurred. However, current approaches for identification of such compounds have proven to be costly, time-consuming and ineffective. This problem underscores the need for a new approach, one that is fundamentally different from traditional methods …

Electrical Characterization Of Blood Clots, William Mcgrath

Master's Theses

This work reports the use of dielectric spectroscopy to study blood and blood clots. A test setup was developed and electrode behavior was characterized with water and salt solutions. By measuring changes in electrical permittivity, trends differentiating clotting and non-clotting blood were identified. Non-clotting blood exhibited an increase in normalized permittivity at high frequencies that is credited to the sedimentation of red blood cells (RBCs) in static blood samples. Clotting blood was found to show a decrease in permittivity after some time at high frequencies. This is associated with the aggregation of red blood cells. Platelet rich plasma (PRP) and …

Non-Invasive Hyperglycemia Detection Using Ecg And Deep Learning, Renato Silveira Cordeiro

Master's Theses

Hyperglycemia is characterized by an elevated level of glucose in the blood. It is normally asymptomatic, except for an extremely high level, and thus a person can live in that state for years before the negative - sometimes irreversible - health impacts appear. Unexpected hyperglycemia can also be an indication of diabetes, a chronic disease that, when not treated, can lead to serious consequences, including limb amputations and even death. Therefore, identifying hyperglycemic state is important. The most common and direct way to measure a person’s glucose level is by directly assessing it from a blood sample by pricking a …

Start-Up Power Requirements Of Ammonia Filled Aerospace Thermosyphon Heat Pipes, Eric Chu

Master's Theses

Boiling and condensing of internal working fluids are essential heat transfer concepts which heat pipe technology relies on for transporting a large amount of heat between two distanced points effectively. Tilt angle and working fluid saturation temperature have a direct impact on how well the evaporator is flooded. This paper focuses on how the tilt angle and the working fluid saturation temperature affect the start-up heat input requirements for an S shaped axial grooved ammonia thermosyphon heat pipe. A series of experiments was performed by supplying and extracting heat to the evaporator and condenser of the thermosyphon heat pipe. Temperature …

Observations Of Fire Behavior On A Grass Slope During A Wind Reversal, Dianne Hall

Master's Theses

This experiment studied fire-atmospheric interactions and wildland fire behavior on a slope. A grass slope was instrumented with both in situ and remote instruments to record both meteorological conditions and the fire behavior. A headfire was lit and allowed to burn upslope through the instruments. The data collected were analyzed to determine the fire behavior, specifically fire spread (direction and rate) and flame characteristics (length, height, and angle). During the first several minutes of the experiment, fire behavior was as expected with an upslope rate of spread at 0.1 m s-1 and flame lengths between 1 m and 4 m. …

Expression And Purification Of Snake Antivenom Peptide In Pichia Pastoris, Israel Juarez Contreras

Master's Theses

It has been shown that a peptide of the first 10-15 N-terminal amino acids of the lethal toxin neutralizing factor (LTNF) protein found in opossums (Didelphis spp.) holds promise as a low-cost therapy for snake envenomation. To date, the 11-mer has been expressed in E. coli and shown to neutralize snake venoms. However, possible endotoxin concerns warrant an investigation into other microbial hosts. The methylotrophic yeast, Pichia pastoris, shows promise as an alternative host. Active LTNF peptide was expressed and purified in P. pastoris. This was accomplished by subcloning a tandem repeat of the first 15 N-terminal amino acids of …

The Deformability Of Red Blood Cells In Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (Me/Cfs), Brendan Robert Schmidt

Master's Theses

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a disease that causes profound fatigue in its sufferers as well as loss of concentration, headaches, and sleep abnormalities. Unfortunately, the disease is poorly diagnosed, and etiology is not known. Although the ME/CFS patients do not show anemic readings, and their arterial partial pressure is normal, it is possible that their extreme post-exertional malaise stems from poor microvascular perfusion and oxygenation. Previous studies have indicated that the red blood cells (RBCs) in ME/CFS patients show evidence of oxidative damage. We hypothesized that the RBC deformability metrics can serve as a reliable biomarker of ME/CFS …

Atrial Fibrillation Detection From Smartwatch Based Photoplethysmography Signals, Syed Khairul Bashar

Master's Theses

Detection of atrial fibrillation (AF) from a wrist watch photoplethysmography (PPG) signal is important because the wrist watch form factor enables long term continuous monitoring of arrhythmia in an easy and non-invasive manner. We have developed a novel method not only to detect AF from a smart wrist watch PPG signal, but also to determine whether the recorded PPG signal is corrupted by motion artifacts or not. In order to detect the motion and noise artifacts from PPG signal, we have used both the accelerometer signal and variable frequency complex demodulation based time-frequency analysis of the PPG signal. After the …

Exploring Parallel Algorithmic Choices For Graph Analytics, Akif Rehman

Master's Theses

Sequential graph algorithms are implemented through ordered execution of tasks to achieve high work efficiency. Exposing parallelism in these ordered workloads tends to be an elusive problem. Strict-ordered parallel implementations find nodes that don't have read-write dependencies and hence can be executed in parallel. They have the work efficiency of their sequential counter-parts due to strict ordering constraints. Larger amount of parallelism can be achieved at the expense of redundant work. Relax-ordered implementations remove the global order and only impose the local order. They go through multiple iterations and have the property of monotonically increasing or decreasing output values allowing …

Unfamiliar Territory: A Preliminary Exploration Of The Experiences Of International Junior Transfer Students, Nicole Mcintyre

Master's Theses

Over one million international students attend American institutions of higher education each year. For many of these students, community colleges are an accessible and increasingly popular enrollment option because they offer small class sizes, intensive English language courses, and affordable tuition costs. Many international students enrolled in community college seek to transfer to a four-year university and complete a Bachelor’s degree. To date, the experiences of international students who successfully complete this transfer pathway have gone largely unstudied. As a result, very little is known about the international junior transfer population. This qualitative study utilizes a phenomenology methodology to explore …

Characterization And Membrane Stability Study For The Switchable Polarity Solvent N,N-Dimethylcyclohexylamine As A Draw Solute In Forward Osmosis, Kevin K. Reimund

Master's Theses

No abstract provided.

Sooting Propensities Of Face Gasolines In Counterflow Diffusion Flames, Peter Vannorsdall

Master's Theses

No abstract provided.

A Comparison Of The Army Corps Of Engineers Hydrologic Modeling System And Autodesk Storm And Sanitary Analysis For Hydraulic And Hydrologic Analysis And Design, Foster Josef Heifetz Campbell

Master's Theses

Hydrological and hydraulic effects of urban development is one of the key issues of study for improved water management. The addition of impervious surfaces to once pervious land and re-routing open channels and flow paths can cause flooding or declining water levels within a watershed. Many studies of these issues have found that there can be multiple factors causing hydrological and hydraulic impacts, and it can be hard to analyze and develop effective solutions without appropriate drainage software packages. However, there are multiple software packages available for use, and determining the correct one to use for a specific challenge can …

Hybrid Iii 95th Percentile Large Male Finite Element Model Neck Alteration, Eric Riley Day

Master's Theses

The motivation behind the project was to update the Livermore Software Technology Corporation (LSTC) Hybrid III 95th percentile finite element model, such that the neck assembly response under varying simulated loading conditions equals that of the federally regulated Hybrid III 95th percentile anthropomorphic testing device (ATD).

The family of Hybrid III crash test dummies approximate the physical properties and response of the human body in a frontal automotive crash. The Hybrid III is used to assess the effectiveness of vehicle restraint systems. LSTC offers Hybrid III finite element models for use in their Multiphysics simulation software package, LS-DYNA. The Hybrid …

Development Of An Analysis Tool And Weight Estimation Method For Aircraft Wing Structures, Kevin M. Barton

Master's Theses

The goal of this effort was to build an analysis tool for aircraft wings and incorporate it into a program that optimizes the wing structure for the lowest weight for the conceptual design phase. The analysis tool calculates the internal stresses in primary load carrying members using established analysis methods for semimonocoque beams. Via a Graphical User Interface (GUI) built in MATLAB, the user can define the structural layout and material properties of the load carrying members. The program requires a degenerate geometry model built with Vehicle Sketch Pad (OpenVSP) to define the outer mold line (OML) of the wing, …

Design, Modeling And Control Of A Two-Wheel Balancing Robot Driven By Bldc Motors, Charles T. Refvem

Master's Theses

The focus of this document is on the design, modeling, and control of a self-balancing two wheel robot, hereafter referred to as the balance bot, driven by independent brushless DC (BLDC) motors. The balance bot frame is composed of stacked layers allowing a lightweight, modular, and rigid mechanical design. The robot is actuated by a pair of brushless DC motors equipped with Hall effect sensors and encoders allowing determination of the angle and angular velocity of each wheel. Absolute orientation measurement is accomplished using a full 9-axis IMU consisting of a 3-axis gyroscope, a 3-axis accelerometer, and a 3-axis magnetometer. …