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The Effects Of An Acute Dose Of Cannabidiol On Health And Two-Mile Time Trial Performance, Elyssa Bell

Master's Theses

As more studies exploring the therapeutic effects of cannabidiol (CBD) in both clinical and non-clinical populations continue to increase, there is a renewed interest in the ability of CBD to affect performance. In fact, many anecdotal reports in the media and online suggest that CBD may improve performance. Unfortunately, very few studies support these claims and research in the area is limited. Purpose: The purpose of this study is to explore the effects of an acute dose of CBD on anxiety symptoms, GI distress, heart rate variability (HRV), heart rate (HR), blood pressure (BP), blood lactate (BL) rate of perceived …

Determining Spatiotemporal Regulation Of Actin Dynamics In Choroid Fissure Closure, Cristal Gonzalez

Master's Theses

There are several stages of development necessary for proper vision. During vertebrate eye development, early eye morphology progresses in three stages: optic cup morphogenesis, basement membrane breakdown, and tissue fusion. Eye development is an intricate collaboration between many cells and requires tissues to migrate and bridge together. The following research investigates the Danio Rerio eye development to understand the molecular regulation of choroid fissure closure (CFC). It is hypothesized that CFC requires temporal and spatial regulation of actin dynamics. We investigate this question using chemical inhibitors, cytochalasin D, an actin treadmilling inhibitor, and NSC23766, a Rac-GEF inhibitor of the actin …

Effects Of Extreme Starvation On The Fasting-Adapted Western Rattlesnake (Crotalus Oreganus), Brandon R. Kong

Master's Theses

Ambush-hunting snakes have long been of great interest in the biology of fasting and starvation, as they are adapted to withstand well over a year without taking a meal. Well-justified ethical constraints have limited our understanding of their biology at the limits of these abilities. Unfortunate circumstances, in which the California Department of Fish and Wildlife confiscated over 50 Western Rattlesnakes (Crotalus oreganus) from an animal hoarder that had long neglected them, culminated in the euthanized animals ending up in the hands of our lab. Some of these snakes were extremely starved, being only one-half to one-third of …

Effect Of Enzymatic Pretreatment On Biomethane Production From Olive Pomace, Ningjing Zhong

Master's Theses

In 2023, approximately 2.36 million metric tons of olive oil were produced globally. Olive pomace, a byproduct of the flesh and pits left after olive oil extraction, presents environmental challenges when used as landfill due to its high polyphenol and organic contents, or when combusted due to greenhouse gas emissions. Its potential as animal feed is limited, yet it holds promise for methane production via anaerobic digestion (AD), providing a source of renewable energy. However, the highly crystallized polysaccharides in olive pomace, such as cellulose, hemicellulose, and pectin, impede its conversion to methane, and the high polyphenol content inhibits methanogen …

Antimicrobials Produced By Ants: Chemical Properties And Microbe Specificity, Katy Chon

Master's Theses

Antibiotic resistance is a growing public health concern worldwide, and for over a decade, we have been witnessing the growth of difficult to treat infectious diseases caused by bacteria that are becoming resistant to antibiotics. This resistance can be mostly related to the misusing and improper use of antibiotics in both humans and animals. To restore this problem, humans have turned to new sources for the production of antibiotics. In this study, we focused on using ants. Social insects, including ants and bees, have faced strong disease pressures during their evolution and have developed a range of methods to fight …

Novel Antivirals Unveiled Against Acute And Persistent Coxsackievirus B3 Infection Via Rapid Screening, Maria Del Mar Villanueva Guzman

Master's Theses

Enteroviruses cause 10 to 15 million infections annually in the United States, and Coxsackievirus B3 is one of the most commonly reported. Coxsackieviruses may become persistent, characterized as a viral infection that is not cleared from host cells and that generates a continuous infection. Patients who develop persistent CVB3 infection may not respond to the same antivirals as an acute infection, which may be detrimental. Therefore, there is a need for broad-range antiviral drugs to combat acute and persistent CVB3 infection, as there is no well-accepted treatment available. We developed a model system to study persistent CVB3 using a pancreatic …

Novel Antivirals Unveiled Against Acute And Persistent Coxsackievirus B3 Infection Via Rapid Screening, Maria Del Mar Villanueva Guzman

Master's Theses

Enteroviruses cause 10 to 15 million infections annually in the United States, and Coxsackievirus B3 is one of the most commonly reported. Coxsackieviruses may become persistent, characterized as a viral infection that is not cleared from host cells and that generates a continuous infection. Patients who develop persistent CVB3 infection may not respond to the same antivirals as an acute infection, which may be detrimental. Therefore, there is a need for broad-range antiviral drugs to combat acute and persistent CVB3 infection, as there is no well-accepted treatment available. We developed a model system to study persistent CVB3 using a pancreatic …

Automated Concrete Bridge Deck Inspection Using Unmanned Aerial Systems Collected Data: A Machine Learning Approach, Rojal Pokhrel

Master's Theses

Many national infrastructures, particularly bridges, are becoming older and older day by day. Their safe operation and maintenance require routine monitoring and inspection. There are lots of bridge inspection specifications and manuals to guide inspectors to give the proper recommendations about the bridge structure and safety. These manuals and specifications require bridge inspectors to manually inspect the bridge and recommend maintenance and repair tasks referring to the specific criteria available in the manuals. In time, the application of technology makes many Unmanned Aerial Systems (UAS) advancements during the phase of data collection. For the image processing and analysis of collected …

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Spatial And Abiotic Effects Of Urbanization On Small Mammal Communities, Bri Casement

Master's Theses

Urbanization, the phenomenon of increasing human population density and artificial structures, is known to decrease the richness, abundance, and diversity of native wildlife populations, fragment natural areas, and increase human and human-commensal animal interactions with wildlife. Dynamics of species persistence in habitat fragments is often understood in the framework of island biogeography theory (IBT), with habitat fragments being analogous to oceanic islands. Despite the conceptual similarities between oceanic islands in IBT and urbanized habitat fragments (fragment size, fragment age, and distance to immigration sources), there are important differences. In urban fragments, the matrix is not completely impassible, habitats within fragments …

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Neurogenomics Of Competition And Parental Care In A Socially Polyandrous Shorebird, Tessa Mathur Patton

Master's Theses

Despite sharing largely similar genomes, females and males of the same species exhibit morphological, physiological, and behavioral differences. Sex-role reversal stems from a unique case of sexual selection in which females compete for mates and males conduct the majority of parental duties. We examined the extent to which aggression and parenting may be mediated by different neurogenomic mechanisms by comparing females versus males in the northern jacana (Jacana spinosa), a sex-role reversed shorebird. We hypothesized that sex differences in neural transcriptomic profiles explain sexual dimorphism in behavior. We conducted RNA-Seq in two brain regions within the social behavior network: the …

Assessment Of Georgia’S Royal Catchfly (Silene Regia) Along A Forest-Edge Gradient In A Remnant Prairie Community, Megan Brooks

Master's Theses

Royal catchfly (Silene regia) is a perennial prairie forb known to grow in rocky, alkaline soils within tallgrass prairies of the eastern United States. S. regia is recorded from twelve states, including Georgia, and is now confined to fragmented habitats due to the loss of 95% of its natural environment. The species is now listed as critically imperiled in the state of Georgia. Attempts at reintroduction via outplanting have only shown limited success, necessitating more research on the environmental conditions in which this plant survives in its southern-most distribution. This study investigated the local ecological factors influencing the …

Fleeing Towards Healing: How Cam Can Heal Refugee Communities, Sarah B. Shaalan

Master's Theses

Alternative methods of healing can be found across the globe and are used by all kinds of people including refugees. This thesis will explore the potential benefits of Complementary and Alternative Medicine otherwise known as “CAM” as a supplementary strategy for helping refugees to deal with stress, Post Traumatic Stress Disorder (PTSD) and other harmful effects sustained on their journey and lives as displaced peoples. I will do so by first situating my project in the literature on Eurocentrism in healing practices and the emergence of CAM as an alternative. Then, using extensive primary and secondary sources, supplemented by an …

Neuroinflammation And Gut Barrier Function Post Traumatic Brain Injury, Anne Sullivan

Master's Theses

Traumatic Brain Injury (TBI) is a major cause of morbidity and mortality that afflicts millions of people in the United States annually. TBI mice had significantly increased expression of the pro-inflammatory cytokines and decreased expression of tight junction proteins CLDN5, CLDN12, and OCLD in brain lesion tissue. TBI mice exhibited a significant accumulation of FITC in the middle and distal part of the small intestine compared to sham mice. TBI mice exhibited an increase in pathogenic bacteria Enterobacteriaceae and a decrease in beneficial bacteria lactobacillus. TBI mice exhibited a trend toward an increase in permeability compared to no treatment. These …

The Role Of Micrornas In Lung Inflammation After Alcohol And Burn Injury, Connor O'Neal Guzior

Master's Theses

Acute lung injury (ALI) and the subsequent development of acute respiratory distress syndrome (ARDS) are among the leading causes of post-burn morbidity and mortality. MiRNAs are small non-coding RNA molecules that are known to regulate gene expression and help regulate lung homeostasis. In this study, we profiled four anti-inflammatory miRNAs (miR-146a, miR-150, miR-194, and miR-671) in lung tissue following an alcohol and burn injury. We found that miR-146a and miR-671 were significantly downregulated in lungs one day after combined injury, leading to increased lung inflammation and pathology. Biological sex did not have an effect on this pathophysiological process. We also …

Investigating Car T Cell Persistence, Functional Phenotype, And The Associations With Clinical Response In Cd19 Car T Cell Treated Patients, Oliwia Bochnacka

Master's Theses

CAR T cell therapy is the adoptive transfer of autologous T cells genetically engineered to express an artificial T cell receptor. In the clinic, CAR T cells have been most effective in treating hematological malignancies and there have been many efforts to continue improving both the safety and efficacy of CAR T cells. Using patient blood samples from the Loyola CD19 CAR T clinical trial, we studied CD19 CAR T persistence and the cell surface phenotype to identify how CD19 CAR T cells change over time in the blood of treated patients and determine whether there were trends associated with …

Engineering An Injectable Hydrogel With Self-Assembling 3d Vasculature, Kendyl Cohn

Master's Theses

This research developed methods for culturing self-assembling capillaries in an injectable gel as a potential method for vascularizing tissue-on-a-chip models to mimic physiological drug delivery. Additionally, a mathematical model was developed as a tool for understanding nutrient delivery and comparison of potential delivery systems. Organs-on-a-chip provide novel platforms for studying biology and physiology in 3D, allow exploration of tissue engineering on a manageable scale, and serve as models for drug screening and drug-delivery testing.

Methods were first developed for co-culture of endothelial cells and fibroblasts (3T3s or HDFs) in 2D, evaluating culture time, seeding density and ratio of HUVECs and …

San Luis Obispo In A Box: Damage Accumulation And Combined Stressor Matching In Accelerated Uv Testing, Cameron N. Causey

Master's Theses

The goal of service life prediction is to accurately predict the useful lifetime of a coating system in a typical service environment. Field testing is the most true-to-life form of service life testing but requires long exposure times, often not quick enough to readily aid redesign in the product development cycle. As an alternative, accelerated weathering chambers are used to speed up coating failure in a laboratory setting. These devices do indeed accelerate failure but often produce failure modes that are not seen in actual service or produce material rankings that are not reproducible. This work explores the principle of …

Sustaining Sustainable Farming: An Evaluation Of The Reasoned Action And Comprehensive Action Determination Frameworks For Persistence, Jet J. Tan

Master's Theses

This paper investigates the persistence of agricultural practices funded by the California Department of Food and Agriculture's Office of Environmental Farming and Innovation (CDFA OEFI). The central inquiry revolves around determining the most effective behavior model for analyzing persistence, comparing the Reasoned Action Approach (RAA), Comprehensive Action Determination Model (CADM), and CADM augmented with structural variables. The study's methodology integrates literature review, analysis of OEFI-funded practices, and statistical modeling to assess persistence levels. Contrary to existing literature, our findings reveal significantly higher levels of persistence than anticipated. Moreover, through model comparison, CADM augmented with political economic variables emerges as the …

The Effect Of Watts-Strogatz And Barabási-Albert Graphs On Memory Formation, Ethan Irick Wolfe

Master's Theses

Understanding higher level cognitive processes is a central problem in neuroscience. The Neuroidal model provides a useful framework for posing these problems in a computer science context. There has been significant recent work trying to understand memory capacity in the Neuroidal model but this work was done assuming that the network of neurons was an Erdos-Renyi random graph. However the network of neurons in the brain has been shown to exhibit small-world properties, which are not present in Erdos-Renyi graphs. In this research we explore replacing Erdos-Renyi graphs with Watts-Strogatz and Barabasi-Albert graphs in order to more accurately model the …

Improving Aerospace System Robustness Integration Of Singular Value Decomposition And Network Centrality, Ricardo S. Contreras

Master's Theses

This thesis presents an approach to understanding and enhancing the robustness of aerospace systems through the integration of network analysis, Design Structure Matrix (DSM), Singular Value Decomposition (SVD), and robustness simulation techniques. This methodology called the Importance Hierarchy method, provides a comprehensive framework for decomposing the interactions of aerospace systems, focusing on identifying and evaluating critical components and interactions.

The Importance Hierarchy method was used to study the Autonomous Flight Termination Unit (AFTU) by analyzing its components and interdependencies. The AFTU was selected because it recently completed a Preliminary Design Review (PDR), and its design and operational functions were well-known. …

Matrix Approximation And Image Compression, Isabella R. Padavana

Master's Theses

This thesis concerns the mathematics and application of various methods for approximating matrices, with a particular eye towards the role that such methods play in image compression. An image is stored as a matrix of values with each entry containing a value recording the intensity of a corresponding pixel, so image compression is essentially equivalent to matrix approximation. First, we look at the singular value decomposition, one of the central tools for analyzing a matrix. We show that, in a sense, the singular value decomposition is the best low-rank approximation of any matrix. However, the singular value decomposition has some …

A User-Centric Continuous Authentication Modality Evaluation And Selection Scheme, Bharath R. Senthilkumar

Master's Theses

One of one-time authentication’s most prominent vulnerabilities is the possibility of lunchtime attacks. In such scenarios, an adversary could exploit an unattended device with an active session and no measures are taken to prevent them from committing malicious acts. To address this issue, continuous authentication is utilized by continuously verifying whether an individual is a device’s rightful owner through various modalities, with data sourced from sensors. In the current body of research within this rising domain, various single-modal and multi-modal continuous authentication systems exist, that focus on employing unique combinations of modalities and improving existing supervised learning models used to …

Modeling The Effectiveness Of Bioremediation On Methyl Tertiary-Butyl Ether In Groundwater, Logan Bundy

Master's Theses

Methyl tertiary-butyl ether (MTBE) and its degraded form tertiary-butyl alcohol (TBA) are both known carcinogens that have contaminated groundwater aquifers across the United States. MTBE is a synthesized compound, once widely used as an additive in gasoline to increase oxygenation. Because of its popularity, MTBE was released into the environment primarily through fuel combustion and leaking underground storage tanks. These two compounds are known to be recalcitrant to most conventional physico-chemical treatment methods. Previous studies have suggested that bioremediation is effective at degrading MTBE and TBA in contaminated groundwater. Bioremediation involves the injection of oxygen, nutrients, and pre-adapted bacterial cultures …

Predicting Rheology Of Uv-Curable Nanoparticle Ink Components And Compositions For Inkjet Additive Manufacturing, Cameron D. Lutz

Master's Theses

Inkjet additive manufacturing is the next step toward ubiquitous manufacturing by enabling multi-material printing that can exhibit various mechanical, electronic, and thermal properties. These characteristics are realized in the careful formulation of the inks and their functional materials, but there are many constraints that need to be satisfied to allow optimal jetting performance and build quality when used in an inkjet 3-D printer. Previous research has addressed the desirable rheology characteristics to enable stable drop formation and how the metallic nanoparticles affect the viscosity of inks. The contending goals of increasing nanoparticle-loading to improve material deposition rates while trying to …

Assessment Of Caloric And Micronutrient Intakes In Female Collegiate Student Athletes, Rachel L. Victor

Master's Theses

Nutrition can play a key role in athletic performance. Optimal nutrition can help athletes maintain their overall health and well-being and can contribute to training adaptations, recovery, performance, and injury prevention. Many studies focus on the intakes and importance of macronutrients in athletic performance. However, micronutrient intakes may also play important roles. Therefore, the purpose of this study was to assess the calorie and selected micronutrient intakes of female collegiate student-athletes across a variety of sports. The participants were instructed to complete 3-day food records which were analyzed and compared to their individual nutrition needs. Results of this study indicated …

Emerging Diseases, Abiotic Disorders, And Macrophomina Root Rot Management Of California Strawberry, Cooper Calvin

Master's Theses

Strawberry is an economically important crop in California, with an estimated value of $2.68 billion in 2023. In California strawberry production, mitigation of low plant health and yield often focuses on major soilborne pathogens, while the contribution of minor pathogens and abiotic disorders to production shortcomings are often overlooked. The objectives of the first project in this thesis are to determine the pathogenicity of multiple minor pathogens and quantify other biotic and abiotic factors that can reduce plant health such as viruses and soil salinity. Two pathogens of the black root rot complex, Pythium spp. and Rhizoctonia spp. as well …

Red Raspberries Attenuate Blood Glucose Spike Of A High Glycemic Load Breakfast Cereal, Dante J. Martin

Master's Theses

Blood glucose (BG) spikes increase chronic disease risk in those with and without diabetes. The fiber and phytonutrient content of fruit, such as raspberries has the potential to attenuate the BG spike of a high glycemic load (HGL) meal. We examined the effect of red raspberries (RRs) on the BG spike when added to a HGL meal. In this randomized cross-over trial, 16 university students were given breakfast cereal and almond milk (control), the control breakfast with 140g added RRs, and the control breakfast with sugar equivalent to the sugar content of the RRs (50% sugar increase from control). Meals …

Non-Linear Modeling Of Hysteresis In Piezoelectric Actuated Cantilever Beam Using The Bouc-Wen Model, Andrew Donald Maas

Master's Theses

Piezoelectric actuators frequently exhibit a time-dependent behavioral phenomenon known as hysteresis, resulting in a lag in the deformation of the actuator compared to linear models. The presence of hysteresis complicates control systems involving piezoelectric actuators. However, traditional modeling methods for piezoelectric actuated smart structures often treat the piezoelectric patches as linear actuators without considering hysteresis, leading to suboptimal controller performance.

This thesis aims to establish a comprehensive model by integrating the Euler-Bernoulli beam bending model with the hysteresis dynamics induced by two opposing piezoelectric patches attached to a beam. A model expansion method is employed to transform the partial differential …