Digital Commons Network^™

Absence Of Keratin 8 Is Associated With Marked Colon Mast Cell Infiltration, Diana El Bezri Nasser

Master's Theses

Patients with inflammatory bowel disease (IBD) have increased numbers of numbers of intestinal mast cells (MCs). Chymases and tryptases are mast-cell-specific proteases (MCPs) that are reliable indicators of MC degranulation. The role of MCs in the pathophysiology of IBD is poorly understood, and it is unclear whether MCs play a role in patients or in experimental models of IBD. Furthermore, MCs have been characterized only in chemically induced models of colitis. In this study, we characterize MCs in inflamed colons of keratin 8 knock-out mice (K8-/-), a spontaneous model of colitis with primarily an epithelial cell defect. We used histologic, …

Hippocampal And Anterior Cingulate Cortex Volumes In Amnestic And Non-Amnestic Mild Cognitive Impairment, Elizabeth R. Tuminello

Master's Theses

Mild Cognitive Impairment (MCI) is a syndrome thought to fall between cognitively normal aging and dementia. Although much research has investigated the structural neuroimaging correlates of amnestic MCI, little research has been done on the imaging correlates of non-amnestic MCI. Even less research has examined the anterior cingulate cortex (ACC), a region important in executive functions (EFs), in these patients. This study attempted to address this gap by examining hippocampal and ACC volumes among amnestic and non-amnestic MCI patients and cognitively normal controls. Those with amnestic MCI were expected to have smaller hippocampal volumes than controls and those with non-amnestic …

The Distribution Of Gnrh And Erbeta In Pre- And Post-Pubertal Male Rats, Maysaa Nageeb

Master's Theses

The hypothalamic-pituitary- gonadal axis is intrinsic in regulating reproductive function. Each part orchestrates the synthesis and release of a key hormone. At the level of the hypothalamus, Gonadotropin- releasing hormone (GnRH) is released and it is the

most upstream regulator of sexual maturation. GnRH neurons express a type of estrogen receptor, ERbeta, which suggests that estradiol (E2) plays a direct role in modulating GnRH function (Herbison and Pape 2001). The objective of this study was to determine if gonadal steroid hormones regulate the expression of ERbeta and GnRH mRNA in the male brain during pubertal development. In-situ hybridization was used …

Undergraduate Music Student Stress And Burnout, Helen Jane Orzel

Master's Theses

Burnout is fatigue and diminished interest caused by long-term stress. It is characterized by emotional exhaustion, depersonalization, and lack of personal accomplishment. In the university music atmosphere, stress and burnout are prevalent and accepted as part of the culture. Symptoms and causes of general stress and burnout have been well researched, but much less has been presented on college musicians' burnout, let alone how to deal with it. This study examines the sources of stress, burnout, and ways of coping for undergraduate music students.

A questionnaire with both quantitative and open-ended questions was administered during the 2009 spring semester. Participants …

In Situ Detection Of Tbx5 Expression In Developing Chick Embryonic Heart, Vaishali Agarwal

Master's Theses

The Tbx 5 gene codes for a highly conserved transcription factor containing a DNA-binding motif called the T box (or T-domain). Tbx 5 is expressed in the posterior sinoatrial segment of the heart along with the developing forelimb buds. Mutations in Tbx 5 gene are associated with Holt Oram Syndrome (HOS), which is characterized by heart septal defects and forelimb abnormalities. Tbx 5 expression was examined during various stages of heart development in chick embryos using both a double stranded digoxygenin-labeled DNA probe and a single stranded digoxygenin-labeled anti-sense RNA probe (riboprobe). Due to the presence of homology in the …

Localization And Functional Analysis Of Plasmodium Falciparum Genes Pfl2550w And Pff0750w, Carolyn Jane Strobel

Master's Theses

Malaria is a parasitic disease that causes over a million deaths worldwide each year. Understanding development through the parasite's life cycle is necessary to stop disease transmission. As the genetic basis for the crucial transition from the erythrocytic asexual cycle to gametocytogenesis is unknown, we hope to better understand this transition by studying sexual stage genes and their roles in gametocytogenesis. PFL2550w and PFF0750w are genes upregulated during gametocytogenesis that were identified by a whole-genome microarray comparing gene expression between gametocyte-producing and gametocyte-deficient strains. In this study, PFL2550w was shown to be a soluble protein that is exported from the …

Do Family Dynamics Mediate The Relationshp Between Early Pubertal Development And Depression For Girls With And Without Spina Bifida?, Rachel Wasserman

Master's Theses

Girls who experience premature puberty have higher levels of depressive symptoms than their peers (Rierdan & Koff, 1991; Hayward et al., 1997). However, girls with spina bifida (SB) experience different psychosocial changes during puberty. This study proposed two longitudinal, mediated moderation models to investigate whether family variables (i.e., parent-child conflict and emotional distancing) contributed to differences in the connection between early pubertal timing and depressive symptoms for girls with and without SB. 62 families (31 SB, 31typically developing) were recruited for a larger longitudinal study. Constructs were assessed subjectively and objectively with the use of questionnaire and observational data. Findings …

Youth In Substitute Care Presenting With Sexual Behavior Problems, Christopher Zaddach

Master's Theses

The purpose of the current study is to investigate the traumatic history of youths in the foster care system presenting with sexual behavior problems. Moreover, co-occurring trauma symptoms will be investigated as possibly contributing to the severity of sexual behavior problems in this population. The present study has four primary goals: (1) This study will focus on identifying traumatic experiences that are associated with sexual behavior problems in children and adolescents in the child welfare system; (2) Co- occuring levels of trauma symptoms including posttraumatic stress symptoms (PTS) and sexual concerns (SC; Briere, 1996) will be tested as possible moderators …

Posttraumatic Stress Symptom Clusters And Externalizing Problems In Young Urban African American Adolescents, Maria Ann Horn-Rollins

Master's Theses

The purpose of this study was to investigate the associations between five posttraumatic stress symptom (PTSS) clusters and two forms of externalizing problems within and across the middle school years in a low income urban sample of young adolescent African Americans. A secondary aim of this study was to explore moderation effects by gender. Total PTSS positively predicted a little over 58% of the cross-sectional externalizing outcomes and uniquely explained between 5 and 12% of the variance in these outcomes over and above gender and exposure to violence. Total PTSS significantly and positively predicted one-third of the longitudinal outcomes and …

Predictors Of Mental Health Outcomes Among Foster Care Children Receiving Community-Based Services, Alison Merri Dunleavy

Master's Theses

Historically, children served in the foster care system experience less favorable mental health outcomes compared to those in the general population (Anctil, McCubbin, & Pecora, 2006; Burns et al., 2004; Garland et al., 2001; Zima, Bussing, Yang, & Berlin, 2000). As a result, the child welfare system has been described as a "de facto public behavioral health care system" (Lyons & Rogers, 2004), prompting state child welfare agencies to seek to put systems and policies in place to serve the needs of these youth, such as Illinios' statewide community-based program, System of Care (SOC), designed to provide multi-modal services to …

The City Imagined: Race, Place And Identity In The Making Of Urban Church, Jessica M. Barron

Master's Theses

In this study, I will explore the racial and urban imaginary of church leaders and members seeking to create an authentic and relevant church for Chicago's unchurched, young-adult urbanite. In their quest to develop a relevant church, the varied imaginations of the city of Chicago by leaders, volunteers and members come to the forefront. How these imaginations are fostered, incorporated, rebelled against or dismissed brings to light the politics of imagining the imagined city. This paper will discuss these varied, dueling, and sometimes contradictory imaginations informing the construction, operation, and branding campaigns of Urban Church. Particular focus will be paid …

Fish Assemblage Structure In Natural, Channelized, And Restored Sections Of Nippersink Creek, Mchenry County, Illinois, Sarah Ann Zack

Master's Theses

Degradation of aquatic habitats and loss of biodiversity are growing concerns of natural resource managers and the general public. Channelization, the common historical practice of straightening streams and rivers for agricultural interests, has had profound detrimental effects on the biodiversity of lotic fish assemblages. Nippersink Creek, McHenry County, IL is a twenty-three mile stream that flows through an area valued for its fish, wildlife, and invertebrate biodiversity. Although a portion of the stream was channelized in the 1950's, restoration efforts by the McHenry County Conservation District have recently restored historical meanders. Nevertheless, efforts to restore streams and rivers to their …

Temporal Variability Of Bioassessment Indices Used To Evaluate Three Midwestern Streams, Nia M. Haller

Master's Theses

Population, community and functional measures or metrics in rapid bioassessment programs aid in establishing biological criteria for streams and rivers. Each metric measures different aspects of community structure and is important in detecting changes in macroinvertebrate community structure that are influenced by changes in water quality. In this study, temporal variation of nine commonly used bioassessment indices was examined in three midwestern streams. The indices were calculated for each of nine replicate benthic macroinvertebrate samples collected monthly for one year from Cowpie Creek (CC), Nippersink Creek (NC) and Lawrence Creek (LC), McHenry County, Illinois. In practice, the habitat sampled for …

Resource Composition And Macroinvertebrate Resource Consumption In The Colorado River Below Glen Canyon Dam, Holly Ann Alfreda Wellard Kelly

Master's Theses

Physical and biological changes to rivers induced by large dams can significantly alter downstream communities, decreasing the biotic integrity of these rivers. For example, completion of Glen Canyon Dam on the Colorado River in 1963 has altered the downstream ecosystem and contributed to the decline of native fish populations and dramatic changes in the macroinvertebrate communities. Physical changes associated with the dam may also influence the food resources supporting macroinvertebrate production, but this has not been previously measured. For example, autochthonous production is high in the clear tailwaters of the dam, but downstream tributary allochthonous carbon inputs are substantial and …

Morphological Development Of The Axial Skeletons Of Esox Lucius And Esox Masquinongy (Euteleostei: Esociforms), With Comparisons In Developmental And Mineralization Rates., Amanda Maria Burdi

Master's Theses

Developmental morphology of the axial skeleton of Esox lucius (i.e., northern pike and type species) and E. masquinongy (i.e., muskellunge) was investigated. More than 1,000 specimens were examined ranging in size from approximately 10 mm notochordal length (NL) post-hatching juveniles to over 80 mm standard length (SL) foraging sub-adults. Results show that regardless of individual variation, the relative sequence of bone formation and mineralization is consistent between the two species. This consistent developmental pattern enabled construction of an ontogenetic staging scheme of eight developmental stages, each characterized by one defining criterion. The first appearance in cartilage and/or first sign of …

Structure-Function Relationship Studies Of The Udp-Glucose Pyrophosphorylase From Escherichia Coli, Agnieszka Maria Orlof

Master's Theses

UDP-Glucose Pyrophosphorylase (UDP-Glc PPase) is a key enzyme of the carbohydrate metabolic pathway widely used among prokaryotes and eukaryotes. In plants, UDP-Glc PPase is necessary for sucrose synthesis while mammals utilize this enzyme for the production of glycogen. A bacterium such as Escherichia coli uses UDP-Glc PPase for biosynthesis of the lipopolysscharide core which forms a cell wall.

This study focused on UDP-Glc PPase from Escherichia coli which is encoded by both galU and galF genes. The first part of this project investigated amino acids that could play an important role in the function of UDP Glc PPase (GalU). Based …

Diet Overlap And Competition Among Native And Non-Native Small-Bodied Fishes In The Colorado River, Grand Canyon, Arizona, Sarah Ellen Zahn Seegert

Master's Theses

Introductions of non-native fishes are threatening native fish communities in streams and rivers across the United States. The Colorado River especially has experienced numerous species invasions, and native fish populations throughout the basin are in decline. The native fish community in the Grand Canyon has been particularly affected, with half of the native fishes extirpated from the canyon. Many scientists blame interactions with non-native fishes for these declines. However, to date no one has conducted a thorough diet analysis of small-bodied fishes in the system to assess overlap between native and non-native fish diets. I analyzed the diets of native …

Hcnabc Gene Expression In The Soil Bacterium Pseudomonas Putida Ath2-Iri/9 Under Various Culture And Rhizosphere Conditions, Myrna May Biswas

Master's Theses

Pseudomonas putida ATH2-1RI/9, a bacterium that colonizes plant roots, can suppress weed seedling growth of velvetleaf by producing hydrogen cyanide in the rhizosphere. The operon hcnABC constitutively expresses HCN synthase, an enzyme which catalyzes glycine to form carbon dioxide and hydrogen cyanide, a secondary metabolite. Cyanogenesis is influenced by several environmental factors including iron, phosphate, and oxygen-limiting conditions. This study used a hcnABC::luxAB gene fusion in Pseudomonas putida ATH2-1RI/9 involving the insertion of a promoterless luxAB gene into the hcnABC operon, which results in bacterial luciferase expression instead of HCN synthase. Bioluminescence by this reporter strain was compared to cyanide …

Courtship And Copulation In Glaucous-Winged Gulls, Larus Glaucescens, Kelly M. Mcwilliams

Master's Theses

Using video recordings and scan counts, I examined the occurrence, sequences, and durations of behavior and environmental factors associated with courtship and copulation in glaucous-winged gulls, Larus glaucescens. Males and females in a pair had equivalent pre- and post-Mount behavior occurrences and durations. The main courtship sequence was Head Toss -- Courtship Beg/Respond -- Mounted/Mount, and felames appeared to initiate transitions between these pre-Mount behaviors. More Mounts occurred during the egg-laying stage of the breeding season, but copulation duration, wing-flag duration, and cloacal contacts per copulation showed no significant differences across sampling periods. Poisson analysis showed no significant grouping …

Extraction And Characterization Of Lipids From Microalgae Grown On Municipal Wastewater, Matthew W. Hutton

Master's Theses

Based on results of its Aquatic Species Program (1978-1996), which sought to develop algae-to-liquid fuel technology, the U.S. Department of Energy has suggested that algal wastewater treatment may be incorporated into biodiesel production schemes to reduce the operating costs of both processes. the purpose of the current research was to evaluate the triglycerides produced by wastewater-grown algae for their suitability as a fuel feedstock and to investigate the effectiveness of several solvent mixtures and extraction procedures at recovering lipids from fresh algae. The research involved two separate experiments. The first determined the quantity and quality of lipids produced over the …

Techniques For Triggering Germination Of Adenostoma Fasciculatum In Revegetation Projects At Rocky Canyon Quarry, Stephanie B. Scolari

Master's Theses

Rocky Canyon Quarry is a granite mine located in the Santa Lucia Mountains about 5 miles southeast of the city of Atascadero. The Surface Mining and Reclamation Act (SMARA), which passed in 1975, dictates that California mines must rehabilitate lands disturbed by mining operations. Dr. V. L. Holland and his students in the Biological Sciences Department at California Polytechnic State University, San Luis Obispo (Cal Poly) have been working on restoration and revegetation of the Rocky Canyon Quarry since the early 1990’s.

Adenostoma fasciculatum (chamise) dominates the chaparral communities (chamisal chaparral) found in Rocky Canyon. Chamise is the most common …

Point-Of-Use Water Treatment Device For Disaster Relief, Patricia Marie Compas

Master's Theses

A point-of-use water treatment device and an optimal method for its use were developed. The specially-designed vessel is to be used with flocculation and disinfection agents, while also providing for clean water storage. Possible applications would be disaster relief, emergency preparedness, and outdoor recreation.

Single Cell Impedance Measurements Using Microfabricated Electrodes And Labview Graphical Programming, Stephanie Sophia Hernandez

Master's Theses

This Master’s Thesis project consists of the research, design, and fabrication of a system that could perform broadband impedance measurements (1kHz-20Mhz) of single cells using National Instruments Labview data acquisition and programming in coordination with a single cell capture device. Presented first is the background information on cells and their electrical properties, along with background in micro-total-analysis systems as well as impedance spectroscopy. Experimental Methods are then discussed for the electrode design, cellular modeling in COMSOL, fabrication methods, and Labview 8.0 Set-up and programming. Measurements were performed using the single-cell capture device on saline, yeast cells, and a polysterene bead. …

Nutrient Removal By Algae Grown In Co2-Enriched Wastewater Over A Range Of Nitrogen-To-Phosphorus Ratios, Laura Michelle Fulton

Master's Theses

In conventional wastewater treatment, biological nutrient removal (BNR) depends on bacterial assimilation for phosphorus removal and nitrification+denitrification for nitrogen removal, with the resulting loss of the fixed nitrogen resource. Alternatively, treatment by microalgae allows for assimilative removal of both phosphorus (P) and nitrogen (N) thereby avoiding the oxygen demand of nitrification and preserving fixed N for fertilizer use. Paddle wheel mixed high-rate ponds have much higher algal productivity than typical oxidation ponds, but even high-rate ponds often cannot grow sufficient algae to completely assimilate the N and P in domestic wastewater. Algae growth in high-rate ponds is usually limited by …

Laser Scanning Confocal Microscopy (Lscm): An Application For The Detection Of Morphological Alterations In Skin Structure, Shea C. Smith

Master's Theses

Laser scanning confocal microscopy (LSCM) is an optical imaging technique that provides improved resolution and sensitivity over conventional methods of optical microscopy. However, the cost of most commercial LSCM systems exceeds the financial limitations of many smaller laboratories. The design of a custom LSCM created at a fraction of the cost of a commercial model is discussed in this paper.

The increase in the incidence rate of skin cancer in the world today is alarming, as such, it is essential to provide an early, rapid and effective method for in vivo diagnostics of human skin tissue. LSCM is capable of …

The Electrical Properties Of Human Tissue For The Diagnosis And Treatment Of Melanoma Skin Cancer, Glenn Cameron Stante

Master's Theses

This thesis discusses the research, experimental methods, and data gathered for the investigation of a novel method for the diagnosis of melanoma skin cancer. First, a background about human skin tissue is presented. Then, a detailed description of melanoma along with current diagnosis techniques and treatment options are presented. In the experimental methods, the electrical properties of several types of tissue were analyzed, the purpose of which was to discover if a tissue type can be distinguished by its electrical properties alone. This would allow for the diagnosis of melanoma to be done by examining the electrical properties of the …

Dissolved Organic Matter Influences The Timing Of Embryonic Development Of The Purple Sea Urchin, Strongylocentrotus Purpuratus., Corbin J. Hodges

Master's Theses

Marine dissolved organic matter (DOM) comprises one of the largest carbon reservoirs on earth and has long been considered a potential energy source for marine invertebrates. The importance of DOM transport has been adequately demonstrated for unicellular organisms, where DOM can meet 100% of an organisms energy needs, but the effects of DOM uptake for marine metazoans are less well understood. In this study, three general areas involving the influence of DOM transport to marine invertebrates were explored. First, we assessed the effects of using seawater exposed to high intensity ultraviolet radiation (UVR) on the study organism; embryos of the …

Arroyo Toad (Anaxyrus Californicus) Life History, Population Status, Population Threats, And Habitat Assessment Of Conditions At Fort Hunter Liggett, Monterey County, California, Jacquelyn Petrasich Hancock

Master's Theses

The arroyo toad (Anaxyrus californicus) is a federally endangered species found on Fort Hunter Liggett, Monterey County, California. The species was discovered in 1996 and was determined to occupy 26.7 km of the San Antonio River from approximately 2.4 km northwest of the San Antonio Mission de Padua, to the river delta above the San Antonio Reservoir. The construction of the San Antonio Reservoir dam in 1963 isolated this northern population of arroyo toads. Through time, the Fort Hunter Liggett landscape has changed drastically. The land was heavily grazed by cattle until 1991, which considerably reduced vegetation in riparian areas. …

A Computational Model Of Apoptotic Osteocyte Correlation To Cortical Bone Remodeling Parameters, Michael Stephen Alexander

Master's Theses

The onset of osteoporosis caused by aging, disease states, and post-menopausal conditions significantly impacts patient quality of life, required healthcare funding, personal autonomy losses from increased fracture risk and the subsequent corrective surgery. Research has indicated that osteocyte apoptosis may be a key parameter in bone remodeling, raising the possibility of remodeling rate modulation for the mitigation of bone mass resorption. By developing therapies that target osteocyte apoptosis, it may be possible to prevent undesired bone remodeling activity while maintaining a healthy balance between damage formation in the form of microcracks induced by the strain environment and the removal of …

The Effect Of Embryo Biopsy And Vitrification On The Development Potential Of Equine Embryos, Richard O. Gearhart

Master's Theses

This study investigated the development potential of equine embryos in vitro after biopsy and vitrification. Twenty embryos were obtained from Quarter Horse, Thoroughbred, and mix-breed light mares between three and ten years old. The twenty embryos were divided into a biopsy (n=10) and control group (n=10). The biopsy group underwent microaspiration biopsy using a micromanipulator to obtain a small tissue sample from the embryo. Both groups were then vitrified using a commercially available technique originally described by Carnevale (2006) at Colorado State \ University.

All 20 embryos were cultured in DMEM/Hams F-12 medium under oil at 37°C in 5% CO2 …