Digital Commons Network^™

The Development Of A Novel Technique To Evaluate Binding Between Probiotic Bacteria And Phospholipids, And The Creation Of A Dairy-Based Food Product Rich In Milk Bioactives, Megan Ann Cleveland

Master's Theses

Probiotic bacteria are increasingly prevalent in food and nutritional products today.  These remarkable microorganisms are capable of imparting exceptional health benefits on their host, including prevention of infection by pathogens and stimulation of immune system function.  Their most common mode of delivery is through dairy products (e.g. yogurt), which are also one of their preferred habitats.  The interactions between probiotic bacteria and dairy systems have been studied, but are still not well discerned.  There is a need for better understanding of these associations, as well as those surrounding the mode of bacterial transfer from the food product to the human …

Intake, Apparent Digestibility, And Digesta Passage In Leopard Tortoises (Geochelone Pardalis) Fed A Complete, Extruded Feed, Laura Evelyn Lickel

Master's Theses

The influence of feeding juvenile female leopard tortoises (Geochelone pardalis, n=18) a commercially available, complete, extruded feed three (3) or seven days (7) per week on dry matter and digestible energy intake, apparent digestibility of dry matter, organic matter, gross energy and fiber fractions, animal body weight and measurements, digesta transit time, rate of passage, and indigestible fill was evaluated.  Both feeding frequencies are commonly practiced with captive tortoises. When fed 7 compared to 3 days per week, dry matter and digestible energy intake was greater.  Tortoises gained more g BW, but not when adjusted per kg initial …

Characterization Of The Equine Spermadhesin Hsp-7 Found On Stallion Spermatozoa As It Relates To Stallion Fertility And Sperm Capacitation, Melodee Kathleen Heidmiller

Master's Theses

Equine spermadhesin HSP-7 is a 14 kDa protein isolated from stallion seminal plasma and present on the surface of spermatozoa. HSP-7 displays carbohydrate and zona-pellucida binding properties, but the physiological role in equine fertilization is not well defined. HSP-7 has 98% amino acid sequence homology with the well-studied boar spermadhesin, AWN. Currently, these two proteins are considered to have the same reproductive function. Immunofluorescence studies presented here show that the stallion and boar spermadhesins are localized to different segments on spermatozoa. The variation in molecular compartmentalization of spermadhesin molecules in different species suggests that these structurally related proteins could be …

Water And Air Quality Performance Of A Reciprocating Biofilter Treating Dairy Wastewater, Seppi Matthew Henneman

Master's Theses

ABSTRACT

Water and Air Quality Performance of a Reciprocating

Biofilter Treating Dairy Wastewater

Seppi Matthew Henneman

Agricultural non-point source pollution is the leading water quality problem in surface water and the second leading problem in ground water in the US.  Among the contaminants, nutrients (such as nitrogen, phosphorus, potassium) can be transported from agricultural fields when cropland is not managed properly.  In California, dairy manure application to cropland has become tightly regulated with the goal of decreasing such nutrient pollution.  Dairies unable to balance their manure nutrient supply with cropland application area may benefit from a nitrogen removal technology.  One …

A Study Of Osteocyte Apoptosis By Region And Quadrant In Murine Cortical Bone, Jessica Kristen Chan

Master's Theses

ABSTRACT

A Study of Osteocyte Apoptosis by Region and Quadrant in Murine Cortical Bone

Jessica Kristen Chan

Osteocytes undergo apoptosis to spatially and temporally initiate bone remodeling.  This study investigates the distribution of apoptotic osteocytes within different quadrants and regions of cortical bone and compares the frequency of osteocyte apoptosis to regional factors associated with bone remodeling.  Specifically, the quantity of apoptosis was compared to levels of the bone morphagenic protein antagonists noggin and gremlin.  Samples of unloaded right tibial bone obtained from C57/Bl/6 mice underwent TUNEL staining for apoptotic osteocytes and were counterstained with methyl green to detect osteocyte …

Pcr Primers For The Detection Of Propane And Butane-Oxidizing Microorganisms, Brian Jeremy Chan

Master's Theses

In an increasingly energy-hungry world, our capacity to meet the heightened energy demands of the future has become a pressing matter. The most urgent of these concerns are tied to the accessibility of petroleum. Various experts have proselytized both the imminent arrival of peak oil production rates and the ensuing decline of those rates thereafter. And to that end, the development of novel and advanced oil exploration methodologies has become almost as important as finding the sources of oil themselves.

The soils above petroleum reservoirs play host to various communities of alkane- oxidizing bacteria that can utilize the natural gas …

Effects Of Season, Size And Parasitism By The Acanthocephalan, Profilicollis Altmani, On The Carotenoid Concentration And Composition Of The Pacific Mole Crab, Emerita Analoga, Lauren F. Constancio

Master's Theses

The Pacific Mole crab Emerita analoga is a filter-feeding crustacean inhabiting sandy beaches along the western coasts of North and South America. The tissues of E. analoga contain carotenoids, dietary pigments that are involved in crustacean cryptic coloration and egg development. Despite extensive study of its life history, little is known about how E. analoga is affected by parasitism, even though previous groups noted the presence of larval helminths in its body cavity. We examined parasite prevalence and intensity of the acanthocephalan Profilicollis altmani infecting E. analoga from Pismo Beach, California. We investiged the effects of parasite infection and body …

Growing Conflict: Agriculture, Innovation, And Immigration In San Luis Obispo County, 1837–1937, Douglas P. Jenzen

Master's Theses

The history of San Luis Obispo and its surrounding areas is complex. Agriculture, innovation, and immigration have all contributed to the formation of the region. The Spanish, Mexican, and early American periods established the framework successive waves of immigrants had to live within. Native Americans and immigrants from China, Portugal, Switzerland, Japan, the Philippines, and other regions of the United States have all toiled in the fields and contributed to America’s tables at various points throughout county history. Many contingencies determined the treatment of successive waves of immigrants. Growth and development are taking place at exponential rates on the very …

Predicting Articular Cartilage Constituent Material Properties Following In Vitro Growth Using A Proteoglycan-Collagen Mixture Model, Michael Stender

Master's Theses

      A polyconvex continuum-level  proteoglycan Cauchy stress function was developed based on the continuum electromechanical Poisson-Boltzmann unit cell model for proteoglycan interactions. The resulting proteoglycan model was combined with a novel collagen fibril model and a ground substance matrix material to create a polyconvex constitutive finite element model of articular cartilage. The true collagen fibril modulus , and the ground substance matrix shear modulus , were varied to obtain the best fit to experimental tension, confined compression, and unconfined compression data for native explants and explants cultured in insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1).  Results indicate …

'Better Make It A Double': Perceived Relatedness Increases Reported Attractiveness, Benjamin R. Ainley

Master's Theses

Sexual selection shaped psychological mechanisms in both sexes to assess potential mates for evidence of mate quality (Buss, 2005). Attraction preferences are one such mechanism (Sugiyama, 2005) and physical attraction preferences are sensitive to fitness-promoting traits present in a potential mate. Physically attractive traits are thought to act as signals of good genetic quality (Neff & Pitcher, 2005) and are preferred because of the advantage such quality bestows towards reproductive success. Specifically, genetic quality is proposed to be a biological requirement necessary for physically attractive traits to develop and be maintained (Johnstone & Grafen, 1993). Furthermore, genetic quality is heritable …

Artificial Neural Networking As A Decision Tool For Natural Gas Investment, Micah D. Denecour

Master's Theses

With the growing interest in the Marcellus Shale and its natural gas deposits, there are opportunities to purchase and hold land for investment purposes. A robust decision tool is needed to help guide investors towards the most profitable properties. Artificial neural networks have many unique benefits that make them an ideal candidate for this purpose.

The artificial neural networks created in this study had nine independent variables. Combinations of these nine variables were created to describe 300 theoretical properties available for purchase. Each of these properties were then evaluated by an expert in the field and given a score from …

Utilization Of Geographic Information System For Research, Management, And Education In The Natural Resources Management Department At California Polytechnic State University, San Luis Obispo, David In Yun

Master's Theses

Geographic Information System (GIS) is “an organized collection of computer hardware, software, geographic data, and personnel designed to efficiently capture, store, update, manipulate, analyze, and display all forms of geographically referenced information” (ESRI, 1997a). The Natural Resources Management Department at California Polytechnic State University, San Luis Obispo, California, hereafter NRM, was one of the early users of GIS technology. Although GIS was primarily used as an educational tool, it was also used for cataloging and displaying resource information for management planning and research projects. As the computing technology advanced, GIS became more powerful and easier to use. NRM faculty and …

Laser Etched Pmma Microfluidic Chip Design And Manufacture With Applications In Capillary Zone Electrophoresis, Evan Allen Barbre

Master's Theses

This thesis encompasses a feasibility study of using low-cost materials to manufacture microfluidic chips that can perform the same functions as chips manufactured using traditional methods within an acceptable range of efficiency of chips created with more exotic methods and materials. The major parts of the project are the selection and characterization of the fabrication methods for creating the channels for fluid flow, the methods for sealing the channels to create a usable chip and the electrophoretic separations of carboxylated microspheres of different potentials. In this work we seek to answer the question if laser-etched PMMA microfluidic chips are comparable …

A Microsimulation Of Traffic, Parking, And Emissions At California Polytechnic State University – San Luis Obispo, Steven Michael Kilbert

Master's Theses

Traffic and parking congestion are significant issues at many universities nationwide. The delays experienced result in wasted time, money, and fuel for students, faculty and staff, not to mention the negative contributions to the environment. This paper quantifies the amount of vehicle emissions generated during an average morning peak hour in the university environment. Using VISSIM and CMEM microsimulation packages, a model is created for California Polytechnic State University- San Luis Obispo to aggregate the collective transportation behaviors and practices of the campus and recognize the implications these behaviors pose on the transportation network as a whole. Reasonable estimates are …

Sar Map Of Gel Phantom In A 64mhz Mri Birdcage By Fiber-Optic Thermometry And Fdtd Simulation, Chirag Mukesh Patel

Master's Theses

As implantable medical devices are being used more often to treat medical problems for which pharmaceuticals don’t suffice, it is important to understand their interactions with commonly used medical modalities. The interactions between medical implants and Magnetic Resonance Imaging machines have proven to be a risk for patients with implants.

Implanted medical devices with elongated metallic components can create harmful levels of local heating in a Magnetic Resonance Imaging (MRI) environment [1]. The heating of a biological medium under MRI is monitored via the Specific Absorption Rate (SAR). SAR, defined as power absorbed per unit mass (W/kg), can be calculated …

The Role Of T-Box (Tbx) 2 In Osteoblast Function, Nidhish Francis

Master's Theses

No abstract provided.

Robust Region Tracking In Multi-Agent Systems Utilizing Sliding Mode Control: Theory And Applications, Mark Bacon

Master's Theses

This thesis presents a methodology to bring controlled agents within a moving region despite agent interaction dynamics, uncertain forces and parameter variation. The logic is derived from traditional Sliding Mode Control theory with an expanded boundary layer which allows position deviation from the region center to specified bounds. As an example of the utility of this control, multiple methods of herding (controlling passive agents by appropriate positioning of controlled agents) are presented.

The Synthesis And Characterization Of Endcapped Silica Hydride Stationary Phase, Prema Gangadhara

Master's Theses

The silanization/hydrosilation process was developed to overcome some of the drawbacks associated with the use of organosilane reagents for the synthesis of high pressure liquid chromatography (HPLC) stationary phases. The first step is called silanization, in which silica or other oxides react with triethoxysilane (TES), resulting in the formation of a silica hydride surface. Endcapping the stationary phase using trimethylchlorosilane (TMCS) and hexamethyldisilazane (HMDS) eliminates the unreacted and accessible silanols on the silica hydride intermediate.

This research employed the modification of bare silica to silica hydride via silanization. Endcapping after silanization eliminated accessible and unreacted silanols. Endcapping was confirmed by …

Food Habits Of Harbor Seals (Phoca Vitulina Richardii) In San Francisco Bay, California, Corinne Michele Gibble

Master's Theses

The diet of harbor seals (Phoca vitulina richardii) in San Francisco Bay (SFB) in California was examined from July 2007 to July 2008 via scat analysis. Scats were collected from five major haul-out sites; 22 species of fish and one species of crustacean were identified from 422 scats. The reliance of a non-native invasive species, Yellowfin Goby (Acanthogobius flavimanus), increased in importance in the diet. Additionally, another non-native invasive fish species, Chameleon Goby (Tridentiger trigonocephalus), was found for the first time in the diet of harbor seals in SFB. Harbor seal diet was compared between seasons, locations, and years using …

The Proteomic Response Of Sea Squirts (Genus Ciona Congeners) To Heat Stress: Evidence For Differential Thermal Sensitivities, Loredana Serafini

Master's Theses

The sea squirts Ciona intestinalis and C. savignyi have disparate distribution patterns, which may result from differences in their thermal tolerance limits. Because C. intestinalis, an almost cosmopolitan species, has a more widespread distribution, it is thought that it is better adapted to endure a wide range of temperatures. In order to compare the heat stress response between these two congeners, we studied global changes in protein expression, using a proteomics approach. To characterize the response to extreme heat stress, animals of both species were exposed to temperatures of 22°C, 25°C, and 28°C for 6 h, and then were …

Life History Characteristics Of The Starry Skate, Raja Stellulata, From California Waters, Kelsey James

Master's Theses

The order Rajiformes, or skates, is a morphologically conservative group that exhibits a wide range of life history characteristics. This inter-species variability warrants species-specific research. This study investigates the age, growth, reproduction, and habitat of the Starry Skate, Raja stellulata, which is endemic to the U.S. West Coast. Age and growth parameters were determined using two preparation techniques: gross sectioning and histological sectioning. The assumption that gross sections deposit one band pair a year was indirectly validated with centrum edge analysis and marginal increment ratio. It was not indirectly validated with histological sections. Age estimates from gross sections ranged from …

Million Dollar Red, Jennifer Marie Ghastin

Master's Theses

The novel Million Dollar Red connects the lives of six women in Eastside San Jose in pursuit of a common set of desires to be beautiful, happy, and something other than what they were when they walked into Quynh's Nail Salon. The third person limited point of view shifts protagonists every time a character comes into contact with a certain shade of red nail polish, Million Dollar Red. According to Quynh, the owner of the salon, the polish has the magical power to give women what they need but not what they want. Once the six women have encountered the …

Post-Fire Analysis Of Sequoia Sempervirens Forests On The Central Coast Of California, Rachel Anne Lazzeri-Aerts

Master's Theses

The public perception of wildfire is that it destroys forests and that all fires should be quickly extinguished. However, fire plays a central role in determining structure, composition, and recruitment in many forest types. In coast redwood forests, the role of fire is not well understood, particularly in the southern part of the range. Observational data suggest that redwoods are relatively unaffected by fire. The sites of three 2008 and 2009 fires were sampled for this study: the Martin Fire in Bonny Doon, California, the Whitehurst Fire near Gilroy, California, and the Lockheed Fire near Swanton, California. Randomly selected ten-meter …

Dangerous Decor: Consumer Knowledge Of Health Risks Within Interior Spaces, Kaitlin Keith

Master's Theses

Interior décor is an ever-present part of people's daily lives. The furnishings and finishes with which people surround themselves are part of their personal expression, but these components in people's homes and work spaces can negatively affect their health. Products such as furniture and paints can contain harmful chemicals, including volatile organic compounds (VOCs) so toxic they are pollutants regulated by the U.S. Environmental Protection Agency. With the rise in the "green" movement, healthy and environmentally safe alternatives such as low or zero-VOC paints are becoming more available. This study assessed how aware consumers and design professionals are of environmentally …

A Mathematical Approach For Predicting Harbor Seal Haul-Out, Jonathan Daniel Cowles

Master's Theses

Harbor seals haul out in response to various environmental factors such as tide, current, time of day, wind, and surf. Mathematical modeling techniques can be used to determine which of these variables are important and to predict the number of seals that will haul-out in a given set of environmental circumstances. Haul-out counts were recorded every hour for 16 hrs per day over two 14-d tidal cycles at a site in Washington State. Deterministic environmental variables (tide height, current velocity, solar elevation, and time of day) were used to create 37 alternative models, which were then compared to the haul-out …

Investigation Of A Pharmaceutical Compound With Artificial Streams: Effects Of The Antihistamine Cimetidine On Stream Ecosystem Function., Paul David Hoppe

Master's Theses

Pharmaceutical compounds have been widely detected in surface waters but their effects on stream ecosystems are unknown. Cimetidine (Tagamet®), a widely used H2 histamine antagonist has been detected in surface waters. To measure chronic effects of cimetidine on stream invertebrates, I conducted a long-term (83d) artificial-stream experiment. A range of cimetidine concentrations (0.07 μg L-1 to 70.0 μg L-1) were added to streams supporting populations of the amphipod Gammarus fasciatus and beetle Psephenus herricki. P. herricki individual growth rates were reduced in the presence of cimetidine, but G. fasciatus individual growth rates were not different among treatments. G. fasciatus size …

Stream Restoration In A Post-Agricultural System: Indirect Effects On Density And Secondary Production Of Aquatic Insects, Lyle Stanley Dandridge Jr

Master's Theses

Restoration of stream habitats, with the goal of increasing biodiversity through increasing habitat heterogeneity, has been an ongoing trend in recent decades. Current investigations suggest most of those projects fail to significantly influence ecological structure and function when evaluated in light of their affect on species richness. In order to assess the <&ldquo>success of restoration on a prairie stream in northern Illinois traditional metrics such as community composition and density were examined in addition to macroinvertebrate secondary production. Restoration of Nippersink Creek, McHenry County, Illinois was completed in 2000 and this study was conducted 8 years post-restoration. Benthic samples were …

Patterns Of Genetic And Morphological Variation In Deer Mice (Peromyscus Maniculatus) In The Warner Mountains, Allison Linnea Wade

Master's Theses

A previously recognized north-south mitochondrial DNA (mtDNA) break in populations of deer mice, Peromyscus maniculatus, was investigated in Western North America. A 383-bp fragment of the mtDNA control region was analyzed from 107 tissue samples in the unstudied Warner Mountains of northeastern California. To determine if this north-south mtDNA break was also reflected in morphology, six cranial and mandibular measurements were taken from 72 deer mice. Using multivariate analysis of variance (MANOVA), it was determined that cranial characters did not vary significantly between the two mtDNA haplogroups, and discriminant function analysis (DFA) was unable to discriminate between the two haplogroups. …

Problem Solving With Geographic Networks In The Sacramento-San Joaquin Delta, Steven Hong

Master's Theses

The Sacramento-San Joaquin Delta (Delta) serves as the crossroads for many geographic networks that crisscross California. These include a conveyance network for drinking water, a biological network for delta smelt, and a transportation network for roads. As a result, local Delta issues concerning flooding and endangered fish hold implications for the entire state. This thesis explores these underlying network structures that pass through the Delta as a means for solving subsets of these most difficult challenges.

Recognition of these larger networks enable the transformation of the Delta's convoluted maze of waterways and islands into a series of nodes and segments. …

Reducing Disturbances To Marine Mammals By Kayakers In The Monterey Bay, Megan Maye Gunvalson

Master's Theses

Team OCEAN is a kayaker-outreach program located in Monterey Bay National Marine Sanctuary under the direction of the National Oceanic and Atmospheric Administration, whose goal is to reduce disturbances to marine mammals by kayakers. This study documented the interactions between kayakers and resting harbor seals (Phoca vitulina) and southern sea otters (Enhydra lutris nereis) at Team OCEAN's two outreach sites, Cannery Row and Elkhorn Slough, to determine if outreach was effective in reducing disturbances to harbor seals and sea otters.

No difference was observed in the percentage of kayaks causing disturbances to resting harbor seals when comparing days Team OCEAN …