Desert Hedgehog Is A Mammal-Specific Gene Expressed During Testicular And Ovarian Development In A Marsupial, William A. O'Hara

Master's Theses

Desert hedgehog (DHH) belongs to the hedgehog gene family that act as secreted intercellular signal transducers. DHH is an essential morphogen for normal testicular development and function in both mice and humans but is not present in the avian lineage. Like other hedgehog proteins, DHH signals through the patched (PTCH) receptors 1 and 2. Here we examine the expression and protein distribution of DHH, PTCH1 and PTCH2 in the developing testes of a marsupial mammal (the tammar wallaby) to determine whether DHH signalling is a conserved factor in gonadal development in all therian mammals.

South Sudan & The “Global Land Grab”: Implications For The Future Of Food Security, Sarah Falltrick

Master's Theses

Abstract

More than 870 million people across the world do not have enough to eat, 98 percent of them live in developing countries. While researchers disagree on the causes of hunger, they all agree that it is a pressing concern. South Sudan occupies some of the most fertile land with access to several major water sources -- all the ingredients for a thriving agriculture sector -- and yet, it is a net food importer. Worse, 60 percent of the population does not consume enough food to provide a nutritious diet. Why does a country with all the best natural endowments …

The Investigation Of The Unc-6/Netrin And Unc-40/Dcc-Mediated Synaptic Partner Recognition Pathway, Kelli Benedetti

Master's Theses

Organisms receive, process, and respond to environmental cues via intricate neural circuits that form during development. Proper development of neuronal circuits is therefore critical to brain function. To form these circuits, a neuron must first extend its axons to the appropriate target. After reaching this target region, it must recognize its correct synaptic partners from multiple incorrect partners and form appropriate synapses. It is well understood how axons reach their target regions, but little is known about how neurons select the correct partners once there, a process called synaptic partner

recognition (SPR). Our work aims to elucidate the genetic programs …

Microsatellite And Mitochondrial Dna Analysis Of Dungeness Crab (Cancer Magister) From California To Northern British Columbia, Bryan Thomas Barney

Master's Theses

Genetic variation was assessed using mitochondrial DNA and microsatellite markers from Dungeness crab between Iceberg Bay, British Columbia and San Luis Obispo, California. We found little pattern in overall genetic variation between sites in both marker types, and no significant Isolation by Distance model was fit. Site-specific variation in mitochondrial DNA haplotype frequencies suggested the existence of three subpopulations associated with the Alaska Current, the Puget Sound, and the California Current, but microsatellite DNA evidence did not support it. The ratio between sampling size for microsatellite markers and fragment size polymorphisms was low, limiting the resolving power of microsatellite DNA …

The Hybrid Laboratory: Informal Spaces For Public-Science Interaction, Whitney Ann Ferrin-Rodriguez

Master's Theses

While growing trends in participatory science and public outreach have made science more accessible to publics, science communities and publics have long interpreted science and laboratories as private domains, perpetuating the idea that science is reserved for "experts" and locations for science are deemed "private." Many studies that examine public-science interaction, however, seldom consider the role of space and place. I argue that material spaces shape public-science interactions. My analysis takes place at an institutionalized informal education platform: a hybrid laboratory: a space used by "expert" astronomers to create new visual knowledge and "lay" publics to actively participate in astronomy. …

Determination Of The Molecular Mechanisms Of Hyperglycemia-Induced Changes In Cav3.2 Calcium Channel Properties, Ginny Marie Keeling

Master's Theses

Hyperglycemia can cause altered excitability due to increased CaV3.2 T-type calcium channel function, bestowing diabetics an increased neuropathy risk. The objective of this study was to understand the molecular mechanisms of increased CaV3.2 function during hyperglycemia in vitro, which leads to a 58% increase in current density and increased channel open probability (PO), among other changes. Two major findings are reported in this study:

1) The enzymatic elimination of extracellular N-acetylneuraminic acid (NANA) moieties reversed the increase in current density and the shift in PO, showing that hyper-glycosylation alters channel function in disease.

2) Treatment with GM1 (a ganglioside highly …

Dynamics Of Vibrio With Virulence Genes Detected In Pacific Harbor Seals (Phoca Vitulina Richardii) Off California: Implications For Marine Mammal Health, Stephanie Nichole Hughes

Master's Theses

Given their coastal site fidelity and opportunistic foraging behavior, harbor seals (Phoca vitulina) may serve as sentinels for coastal ecosystem health. Seals using urbanized coastal habitat can acquire enteric bacteria, including Vibrio, that may affect their health. To understand Vibrio dynamics in seals, demographic and environmental factors were tested for predicting potentially virulent Vibrio in free-ranging and stranded Pacific harbor seals (P. v. richardii) off the coast of California. Vibrio prevalence did not vary with season and was greater in free-ranging seals (29%, n = 319) compared with stranded seals (17%, n = 189). Of …

Determining The Intracellular Concentrations Of Flavonoids In Mda-Mb-231 Cells Using Hplc-Coupled Mass Spectrometry, Anh Dang Pham

Master's Theses

Flavonoids are polyphenolic compounds found in a wide variety of plants. In recent years, flavonoids have been found to be beneficial to human health. Our lab has investigated the ability of flavonoids to induce cytotoxicity in human breast cancer cell lines. We have discovered that some compounds can induce cytotoxicity very readily, whereas others cannot. We hypothesized that this difference is attributable to a compound's ability to transverse the cell membrane. Therefore, we used LC-MS to measure the amount of each flavonoid that is able to enter into MDA-MB-231 cells, human breast cancer cells. We tested 14 flavonoids. MDA-MB-231 cells …

Stirring Up The Fires : John Spencer Bassett, "The Negro Question" Ansd Southern History, Robert Spencer Dicks

Master's Theses

In 1903 John Spencer Bassett, a young history professor at Trinity College, took to his pen to produce, "Stirring up the Fires of Racial Antipathy," a bold article intended to provoke discussion about southern race relations. An intense public backlash followed, nearly costing Bassett his job . The event, known as the "Bassett Affair," made national headlines. Many scholars have referenced the "Bassett Affair" as a triumph for academic freedom or as a part of a larger story about southern dissent. The 1903 controversy, however, was just one episode in the story of this iconoclastic historian. Delving into Bassett's personal …

Growing Greens And Soiled Soil: Trends In Heavy Metal Contamination In Vegetable Gardens Of San Francisco, Jennifer Gorospe

Master's Theses

As urban dwellers prioritize eating locally grown foods to reduce environmental impacts, benefit the local economy, and bring fresh produce to the table, urban gardening has emerged as a popular approach to improving food security. Researchers have shown previously that urban landscapes can accumulate environmental toxins at levels that may be unsafe for human health, with socioeconomically disadvantaged communities often shouldering an unequal burden of exposure. In order to identify and predict likely risk to gardeners, in this study, soil samples from 91 vegetable gardens in the city of San Francisco were analyzed for 16 heavy metals. A majority of …

A Morphological Study Of Eriogonum Nortonii (Polygonaceae) And Implications For Its Biogeography, Lisa Deree Morton

Master's Theses

Morphological characters, phenology, and abiotic parameters were measured to determine whether divergence has occurred between populations of Eriogonum nortonii E. Greene on the east and west sides of the Salinas Valley. Morphological variability was demonstrated across all populations but not between populations on opposite sides of the valley. Investigations into present and paleoenvironmental factors as well as dispersal factors suggest recent episodes of long distance dispersal were responsible for the disjunct distribution of E. nortonii populations.

Coming From Sounds Of Blackness: Exploring The Effects Of Hip-Hop On Views Of Race, Eliezer Bercasio

Master's Theses

Does the individual's frequency of listening to hip-hop music have an effect on whether or not they believe in a color-blind ideology? Using data from a 25-item questionnaire given to 165 participants drawn for a sample from an urban California college, this study tested six hypotheses in order to determine if such a connection exists. Results revealed that individuals who frequently listened to misogynistic rap were less likely to believe that race is a convenient way to categorize individuals, controlling for race, sex, and income. Thus, individuals who had a higher frequency of listening to hip-hop were less likely to …

Climate-Related Floral And Vegetative Size Variation In Leptosiphon Androsaceus (Polemoniaceae), Aggie Morrow

Master's Theses

Phenotypic plasticity in plants is a vital adaptive response to changing environmental conditions. Floral and vegetative morphology often varies as biotic and abiotic factors vary. Variation is the basis of evolution, and natural selection, acting on trait variation, can lead to speciation. For this study, floral and vegetative character traits of false babystars (Leptosiphon androsaceus, Polemoniaceae) were analyzed within four populations at Henry W. Coe State Park in 2011. Traits were measured to assess changes in size along a moisture availability gradient. Stable carbon isotope ratio samples were collected to measure integrated water-use efficiency (WUE) as it related to precipitation …

Ethics And Wolf Management: Attitudes Toward And Tolerance Of Wolves In Washington State, Julie Callahan

Master's Theses

Approximately seventy-five years after extirpation from Washington State, gray wolves (Canis lupus) returned. As of December 2012, eight packs had arrived from adjacent states and provinces. Delisted from the Federal Endangered Species List in the eastern one-third of Washington, state wildlife managers now have the authority to manage wolves without federal supervision. As a result, one seven-wolf pack has been destroyed. The current study was developed to provide information for managers and policymakers to modify wolf management policies to fit the new regulatory context. Effects of a range of cultural and demographic factors on attitudes toward wolves and tolerance of …

An Exploratory Study On The Relationship Between Creativity, Religion, And Religiosity, Kim-Lien Thi Nguyen

Master's Theses

Religiosity and religion are often said to be negative influences on one's creativity level. Creativity and religiosity have been looked at as a single dimension, which is a simplistic view. The purpose of this study was to examine the relationship between creativity and religiosity using scales that measure each construct multidimensionally. Religiosity was measured by one's level of inclusion of transcendent reality and symbolic interpretation of religious content. Creativity was evaluated in terms of fluency, originality, elaboration, abstractness, and resistance to premature closure. It was predicted that participants who exhibit high inclusion of transcendence and literal interpretation of religious content …

Effects Of Stress And Co-Rumination On Creativity And Performance, Subha Govindarajan

Master's Theses

Stress is shown to have a negative impact on individuals, organizations, and society at large. Though research in industrial/organizational (I/O) psychology has examined the relationship between stress and various organizational outcomes, the effects of stress on creativity and performance have seldom been investigated. Furthermore, despite the well-reported buffering effects of social support on the negative outcomes of stress, the potential effects of one type of social support, co-rumination, on organizational outcomes have not been examined. Therefore, using 100 undergraduate students, the effects of stress and co-rumination on creativity and performance were examined. Results did not show that stress and co-rumination …

Socially Constructing God: Gender, Culture, And A Stratified Trinity, Landon P. Schnabel

Master's Theses

The Problem. Both hierarchicalist and equivalentist evangelicals use Trinitarian relations to argue for their opposing views on gender roles. How can this stalemate be broken if the Bible, the typical evangelical foundation for resolving doctrinal disputes, is used by both sides to argue their contrasting positions? -- Methods and Procedures. I explored the cultural determination of worldview, bringing the fields of sociology, psychology, history, and philosophy to bear on this theological debate and considered whether it may be differing presuppositions and subsequent opposing interpretations that cause the conflicting positions, rather than what the biblical text means. By looking through the …

Maintenance Of Polish Language And Culture Among First Generation Polish Immigrants Living In The Southern San Francisco Bay Area, California., Dominika Bialek

Master's Theses

The focus of this thesis research is to investigate the extent to which first

generation Polish immigrants living in the southern San Francisco Bay Area in California maintain their native Polish language and culture. Qualitative data were collected in the form of interviews and survey questionnaires. The major findings show that in this small immigrant community (1) maintenance of Polish language and culture happens primarily at home; (2) females married to first generation Polish immigrants are more likely than others to maintain Polish language and culture; (3) the small size of the Polish community, knowledge of English at the time …

Habitat Use By Myotis Yumanensis And Tadarida Brasiliensis Mexicana In South San Francisco Bay Wetlands: An Acoustic Study, Theresa Brickley

Master's Theses

Research on bat habitat use within coastal estuaries is limited. The purposes of my study were to determine whether Yuma myotis (Myotis yumanensis) and Mexican free-tailed bats (Tadarida brasiliensis mexicana) differentiate between open water and marsh within saline and brackish habitats and to examine whether climatic factors are correlated with general activity and tidal height with foraging of the two species. I recorded echolocation sequences over 30 survey nights in Alviso, California. Two Anabat II^® detectors were randomly deployed each survey night in open salt water and salt marsh or open brackish water and brackish …

Analysis Of Sol-Gel Encapsulated Aggregate-Prone Peptides By Circular Dichroism, Nathan James Birtwhistle

Master's Theses

Protein aggregation has been linked to many debilitating neurological diseases. In each case, a specific protein is thought to have a region of intrinsically disordered structure that seeds the aggregation. Highly cooperative in nature, protein aggregation is difficult to investigate. The current study aims to characterize two aggregation-prone peptides involved in Huntington's disease, polyglutamine (polyQ, D₂Q₁₅K₂), and Alzheimer's disease, amyloid-beta (A&beta). A protocol was developed to encapsulate the peptides by the sol-gel technique. Generated from a liquid state, the silica matrix is presumed to isolate the soluble peptide, preventing aggregation. In addition, the porosity …

Prey And Plastic Ingestion Of Pacific Northern Fulmars (Fulmarus Glacialis) Collected In Monterey Bay, California, Erica Lynn Donnelly-Greenan

Master's Theses

Marine plastic pollution affects seabirds that mistake it for prey or incidentally ingest it with prey. Northern Fulmars (Fulmarus glacialis) and seabirds that feed at the water's surface ingest the most plastic. This can cause health issues, including satiety that possibly leads to inefficient foraging. The objectives of this study were to examine fulmar body condition, identify cephalopod diet and ingested plastic, predict foraging areas, and determine if prey number and size was correlated with ingested plastics in Pacific Northern Fulmars (Fulmarus glacialis rodgersii). Cephalopod prey were identified, measured, and enumerated; plastic and marine debris were categorized, enumerated, and weighed …

(Re)Defining < Marriage >: An Autohistoria Of An Ideograph, Robert Gutierrez

Master's Theses

This study explores as an ideograph in the law and everyday life through an analysis of private and public texts and embodied acts. The purpose of this study was to engage intersectionally as a power structure with wide-ranging influence over society to locate resistance. Utilizing rhetorical methods, this work examined archives and repertoires through an autohistoria of a queer Chicano male before the passage of Proposition 8, the California Constitutional Amendment that banned for LGBTQ couples. By exploring through metaphors and performative writing, this study constructs in the tradition of the shaman to present new possibilities for and queer theory.

Consequences In Alcohol Use Portrayals On Emerging Adult Attitudes, Stephanie Jwo

Master's Theses

Many factors may contribute to forming attitudes towards alcohol, such as peer groups, family history, and media influences. This study focused on the inclusion of consequences in portrayals of alcohol-use and the possible influences on emerging adult attitudes towards alcohol. The emerging adult population represents a relatively newly defined developmental stage that roughly spans the ages of 18 to 25 years. Emerging adult attitudes towards alcohol were expected to vary positively or negatively after reading a vignette of alcohol-use that included a positive consequence, a negative consequence, or no consequence. Emerging adult attitudes did not vary by the alcohol-use consequences …

Health Implications Of Mercury, Selenium, And A Red Pelage In Pacific Harbor Seals (Phoca Vitulina Richardii) Off Central California, Elizabeth Alexis Mchuron

Master's Theses

San Francisco Bay (SFB) is the largest estuary on the west coast of the United States. It provides habitat for many species, although is heavily urbanized with a history of selenium (Se) and mercury (Hg) contamination. Harbor seals (Phoca vitulina) are good indicators of the health of SFB because they are long–lived, upper–level trophic consumers, and present in the estuary year–round. The objective of this study was to examine the role of Se and Hg contamination on the health of harbor seals in this region, and the role of Se in the development of a red pelage. Between 2009 and …

Luminescence Studies Of Europium (Iii) - D-Glucosamine Complexes In Water, Triny Trinh Pham

Master's Theses

LUMINESCENCE STUDIES OF EUROPIUM(III) - D-GLUCOSAMINE

COMPLEXES IN WATER

by Triny Trinh Pham

Lanthanide complexation interactions measured by luminescence have become

interesting topic in recent years. Europium is a rare earth metal and the Eu(III) ion is a hard acid; when Eu(III) and a monosaccharide ligand are present, a complex may be formed. The goal is to determine the equilibrium constant KEuL between the europium ion and a D-glucosamine ligand. At 25oC and pH ~ 7.40, a physiologically important pH, adding D-glucosamine enhanced the luminescence of 0.0025 M Eu(III). As the concentration of ligand increased, the emission intensity was also …

Differential And Temporal Immunomodulation Of Alpha4 Integrins On Cd4+ Memory Cells By Bordetella Pertussis And Bordetella Parapertussis, Dipti Ravindra

Master's Theses

Pertussis, caused by Bordetella pertussis (B. pertussis), is reemerging worldwide due to vaccine inefficacy. The hallmarks of infection are extreme lymphocytosis and delayed recovery, which are partially associated with pertussis toxin. Lymphocytes migrate to infected tissues using trafficking receptors. Specific combinations of these lymphocyte trafficking receptors are identified for skin and gut but are not well established for lung.

This study focused on the effect of pertussis toxin on lung-associated trafficking receptors and tested the hypothesis that pertussis toxin alters dendritic cell imprinting of lung trafficking receptors on T cells, thus delaying resolution of the infection. B. pertussis-infected mice were …

Plasmablast Frequency And Trafficking Receptor Expression Are Altered In Pediatric Ulcerative Colitis, Nicole Jeanne Tarlton

Master's Theses

Background: The incidence of pediatric ulcerative colitis (UC), a chronic autoinflammatory disease of the colon, is on the rise. Although an increased infiltration of B cells from the peripheral blood into the colon occurs in UC, B cell trafficking is understudied. We hypothesized that the frequency of circulating plasmablasts (PBs) and their trafficking receptor (TR) expression may be indicative of the location and degree of pathology in pediatric UC. Methods: We conducted multicolor flow cytometry analyses of circulating IgA+/- PBs and IgA+ memory B cells (MBCs) in pediatric UC patients with remission, mild, moderate, and severe disease (n=12) and healthy …

Feral Cat Management: Perceptions And Preferences (A Case Study), Rachel Lm Wilken

Master's Theses

Feral cat management is a highly debated topic. Trap, Neuter, and Return (TNR) programs have become increasingly popular as an alternative to eradication. Public preference about how to manage feral cats has been explored by previous authors, but no consensus has been reached. Public policy and best management practices depend in part upon public opinion on this topic. Wording of a feral cat management preference in surveys can play a role in respondent opinion. For this thesis, the researcher examined public opinion about feral cat control by surveying 298 Stanford University faculty and staff members using two different communication approaches. …

Effects Of Tissue Nitrogen And Media Nitrate On Trace Metal Uptake And Trophic Transfer By Ulva Spp., Sonya Meenakshi Sankaran

Master's Theses

A general survey of trace metal content in Ulva spp. (Linnaeus) around Moss Landing, California was carried out. The next objective was to evaluate whether tissue nitrogen or media nitrate affects metal uptake (As, Pb, Mn, Zn) by Ulva spp. under eutrophic conditions. Additionally, the role of metal burden in the invertebrate Idotea resecata as a function of metal content in its diet was examined. Mean trace metal concentrations in Ulva differed significantly among sites in Moss Landing, California. Laboratory measurements using samples from 15 sites along the central California coast revealed a significant positive correlation between Ulva spp. tissue …

The Biology And Ecology Of Six Rare Plants From Plumas National Forest, Northern California, Usa, Suzie Woolhouse

Master's Theses

The majority of our knowledge about the natural world has come from the study and observation of common species, yet a significant portion of species in the world are rare. In this study, biological and ecological data was collected for six rare taxa from Plumas National Forest. The taxa were Monardella stebbinsii, Monardella follettii (Lamiaceae), Lewisia cantelovii (Montiaceae), Cypripedium californicum, Cypripedium fasciculatum (Orchidaceae) and Clarkia mildrediae subsp. mildrediae (Onagraceae). The two Monardella's and Cypripedium californicum are serpentine endemics, and the Lewisia is found growing both on and off serpentine. Five sites were visited per species, and soil samples …