Digital Commons Network^™

Efficiency Of Piezotome-Corticision Assisted Orthodontics In Alleviating Mandibular Anterior Crowding - A Randomized Controlled Clinical Trial, Rana Mehr

Master's Theses

ABSTRACT

Objective: The aim of this study was to investigate the duration of mandibular-crowding alleviation with piezotome-corticision orthodontics compared with conventional orthodontics and the accompanying effects on patient’s pain and satisfaction.

Materials and Methods: 14 subjects were selected based on the following inclusion criteria: adult patients 18 or older, single arch or double arch treatment, non-extraction treatment in the mandibular arch, presence of a full complement of dentition from mandibular first molar to first molar, no spaces in the mandibular arch, mandibular anterior irregularity index greater than 5, patient with healthy periodontium and attachment loss of up to 2mm, the …

Talker-Specific Influences On Phonetic Boundaries And Internal Category Structure, Janice Ann Lomibao

Master's Theses

Recent findings indicate that listeners are sensitive to talker differences in phonetic properties of speech, including voice-onset-time (VOT) in word-initial stop consonants. The current work extends our earlier research by examining the degree to which listeners adjust the initial mapping from acoustic signal to segmental representation on a talker-specific basis. Two groups of listeners are exposed to a talker producing “cane.” Word-initial VOTs are manipulated such that one group hears “cane” produced with short VOTs and the other group hears “cane” produced with relatively longer VOTs. Following training, listeners’ voicing boundary for a /g/-/k/ continuum was tested. In addition, listeners …

Escherichia Coli Strain Diversity In Humans: Effects Of Sampling Effort And Methodology, Emily R. Neal

Master's Theses

Studies investigating Escherichia coli strain diversity and demographics in human hosts are frequently inconsistent regarding sampling effort and methodology while current strain typing methods are often expensive or laborious. To rectify these inconsistencies, sampling effort was investigated by comparing the diversity of 15-isolate collections to 100-isolate collections from 3 human subjects. Temporal variation in E. coli strain diversity was also studied by collecting 15 isolates once every 6 months. Additionally, strain identification and diversity collected by different sampling methods (fecal swabs vs. anal swabs collected at different times around defecation) were compared to identify any inherent biases in sampling method. …

Evaluating Usability Evaluations, Allen Leo Dunlea

Master's Theses

We live in an age when consumers can now shop and browse the web using hand-held devices. This means that competitive companies need to have a website to represent their brand and to conduct business. E-commerce sites need to pay special attention to the usability of their sites, since it has such an impact on how potential costumers view their brand.

Jakob Nielsen defines usability as a "quality attribute that assesses how easy user interfaces are to use"; he separates usability into five quality components: learnability, efficiency, memorability, errors and satisfaction. The current standard for testing usability involves having …

Dna Purification Process Optimization At Life Technologies Corporation, Trevor J. Shepherd

Master's Theses

This project focused on optimizing the plasmid DNA purification process at Life Technologies. These plasmids are designed to code for specialized proteins used by research universities, national laboratories, or research companies. Once cultivated and harvested, the plasmids must be analyzed for quality and quantity. The project is divided into improving three aspects of the process: 1) plasmid identification, 2) plasmid purity evaluation, and 3) process yield. Plasmid identification is now simpler, more robust and has zero ambiguity. Plasmid purity evaluation is now measured with computer software, which reduces user error and eliminates subjectivity. Using the nascent metrics provided by the …

Cplop: The Cal Poly Library Of Pyroprints, Jan Lorenz Villaceran Soliman

Master's Theses

To date, microbial source tracking (MST), i.e. determining the source of microbial contamination based on the specific strains observed in the environment, often uses methods that are time-consuming, expensive and not always reliable. The biology department at Cal Poly, San Luis Obispo has developed a new method for MST called pyroprinting. Pyroprints can be used as DNA fingerprints for identifying sources of fecal contamination and studying bacterial populations in host animals. The MST method consists of two parts: the pyroprinting process and a database of pyroprints. The Cal Poly Library of Pyroprints (CPLOP) was developed to provide a database application …

Computational Modeling To Evaluate Helical Electrode Designs For Use In Vagus Nerve Stimulation, Anthony W. Cowley

Master's Theses

An estimated 0.5% of world’s population has been diagnosed with epilepsy. Of these patients 20-30% will be unable to achieve seizure control with anti-epileptic drugs. Vagus nerve stimulation (VNS) may be an appropriate treatment option for some patients with pharmaceutically refractory, partial-onset seizures.

VNS therapy uses a helical electrode to interface between the implantable pulse generator and the vagus nerve. While there have been several studies related to the mechanical and electrical safety of such electrodes, little work has been done toward understanding the effectiveness of the helical electrode in nerve stimulation. A better understanding of the voltage field and …

Latent Cysteine Residues From Polymers Prepared Via Free And Controlled Radical Polymerizations, Douglas Vincent Amato

Master's Theses

One less commonly used “click” reaction is thiazolidine chemistry. Thiazolidine chemistry is a commonly used reaction used in biological systems because the reaction requires the presence of both cysteine (a common amino acid) and an aldehyde or ketone. If cysteine residues could be incorporated into a polymer then a variety of applications could be developed. Polymers containing free thiols (aka thiomers) have developed in the last decade to become great mucoadhesives. If there was a facile route to control the amount of free thiols along the polymer then more fine-tuned and potentially stronger adhesives could be made. For these reasons …

Testing Subspecies Limits In Monardella Villosa, Taylor Mclaughlin Crow

Master's Theses

Several subspecies of Monardella villosa (California Coyote Mint) have been distinguished on the basis of leaf thickness, shape, and trichome characteristics, yet many intermediates are known. We investigated morphological differences in natural populations of two subspecies (M. v. subsp. villosa and M. v. subsp. franciscana) in the Scott Creek watershed, north of Davenport, in Santa Cruz County, CA. Monardella villosa subsp. franciscana grows in coastal scrub in gulches and ocean terraces whereas subsp. villosa grows at more protected inland sites. Morphological difference between subspecies may be adaptations to these different habitats. I grew plants in a common garden at Cal …

Dairy Manure Flushwater Treatment By Packed-Bed Anaerobic Digesters, Neal Cary Adler

Master's Theses

Wastewater treatment performance of three pilot-scale packed-bed anaerobic digesters with walnut shell medium was researched for treating dairy freestall barn flushwater. Reciprocation mixing was evaluated as a means to lessen channelization in the media bed and to improve biogas production and organic matter removal at ambient temperatures. Reciprocation has been used in biological nitrogen removal systems to introduce air into the system to repeatedly oxygenate nitrifying biofilm along with mixing (Behrends et al. 2003), but the anaerobic systems benefit from mixing. Two tanks were used in each system, where one was full and one was empty at any given time. …

Nutrient Removal Using Microalgae In Wastewater-Fed High Rate Ponds, Matthew N. Rodrigues

Master's Theses

This thesis discusses the mechanisms associated with the removal of organic matter, nitrogen and phosphorus in wastewater-fed high rate algae ponds (HRAP) designed to operate as triplicates. Research was conducted at the San Luis Obispo Water Reclamation Facility (SLOWRF) as a pilot-scale study of nine 30-square meter ponds one foot in depth. During period of study, triplicates were operated at hydraulic retention times (HRT) of 4, 3 or 2-days. Main objectives for the study were to determine minimum HRTs required to achieve secondary and tertiary treatment. Experimental conditions such as CO₂ supplementation, nighttime aeration and operation of ponds in …

Establishment And Validation Of A Novel Fucci (Fluorescent Ubiquitination-Based Cell Cycle Indicator) In An In Vitro Chronic Myeloid Leukemia Model, Jane Marie Isquith

Master's Theses

The deregulation of the cell cycle is important in the leukemic transformation and in the continued discovery of more efficient targeted therapies. After elucidation of the role of the RNA editor ADAR1 in the malignant reprogramming of Chronic Myeloid Leukemia (CML) progenitors to leukemia stem cells (LSCs) during the chronic phase to blast crisis stages of the disease, current research seeks to uncover the mechanism of action of ADAR1 in this process. To study the potential role ADAR1 plays in the changes to cell cycle during this disease progression, a novel Fucci (fluorescent ubiquitination-based cell cycle indicator) system was introduced …

Shark Sim: A Procedural Method Of Animating Leopard Sharks Based On Raw Location Data, Katherine S. Blizard

Master's Theses

Fish such as the Leopard Shark (Triakis semifasciata) can be tagged on their fin, released back into the wild, and their location tracked though technologies such as autonomous robots. Timestamped location data about their target is stored. We present a way to procedurally generate an animated simulation of T. semifasciata using only these timestamped location points.

This simulation utilizes several components. Input timestamps dictate a monotonic time-space curve mapping the simulation clock to the space curve. The space curve connects all the location points as a spline without any sharp folds that are too implausible for shark traversal. We create …

Calcium Carbonate Particle Size Effects On Titanium Dioxide Light Scattering In Coatings, Jason W. Boke

Master's Theses

Titanium dioxide (TiO2) is an essential component in a white paint formulation due to its excellent light scattering ability. TiO2 also happens to make up most of the raw materials cost. Viable replacements to TiO2 do not currently exist in a feasible manner. Rising costs in the recent TiO2 supply shortage have forced coatings formulators to find a balance between cost and performance. One method includes partial substitution of TiO2 with cheaper material such as calcium carbonate (CaCO3), which serves to occupy volume.

The purpose of this study was to compare the coating spread rate of paints with varying TiO2 …

Surfactant Formulations For Water-Based Processing Of A Polythiophene Derivative, Cameron Dean Danesh

Master's Theses

Conjugated polymers are semiconducting materials that are currently being researched for numerous applications from chemical and biological sensors to electronic devices, including photovoltaics and transistors. Much of the novel research on conjugated polymers is performed in academic settings, where scientists are working to prepare conjugated polymers for commercially viable applications. By offering numerous advantages, inherent in macromolecular materials, conjugated polymers may hold the key to cheap and environmentally friendly manufacturing of future electronic devices. Mechanical flexibility, and solvent-based coating processes are two commonly cited advantages. Transitions in the backbone conformation of polythiophenes (PT) in organic solvents have been widely observed …

Algorithms For Library-Based Microbial Source Tracking, Aldrin Montana

Master's Theses

Pyroprinting is a novel, library-based microbial source tracking method developed by the Biology department at Cal Poly, San Luis Obispo. This method consists of two parts: (1) a collection of bacterial fingerprints, called pyroprints, from known host species, and (2) a method for pyroprint comparison. Currently, Cal Poly Library of Pyroprints (CPLOP), a web-based database application, provides storage and analysis of over $10000$ pyroprints. This number is quickly growing as students and researchers continue to use pyroprinting for research. Biologists conducting research using pyroprinting rely on methods for partitioning collected bacterial isolates into bacterial strains. Clustering algorithms are commonly used …

Analysis Of Protein Pre-Coatings And Their Effect On Endothelial Cell Adhesion In A Tissue Engineered Blood Vessel Mimic, Rachel Ann Gohres

Master's Theses

Cardiovascular disease is the leading cause of death in developing countries. Because thousands of people are affected by this disease, medical device companies are constantly developing new intravascular devices in attempt to cure or alleviate the symptoms of the disease. However, before these devices can be brought to market or implanted in to patients, they must complete three stages of testing to receive FDA approval. These testing stages include: in vitro testing, in vivo testing, and clinical trials. Currently, there is a large gap between in vitro and in vivo testing because it is difficult to obtain physiologic information about …

Assessing Physiological Thresholds For Eelgrass (Zostera Marina L.) Survival In The Face Of Climate Change, Carolyn Jane Ewers

Master's Theses

Seagrasses are well known for the important ecological roles they play in coastal marine waters worldwide. However, the severe rate of decline observed in seagrasses this century is expected to accelerate with climate change. Conservation efforts can be improved by quantifying physiological thresholds of seagrasses and using these estimates in modeling to forecast changes in distribution. This study examines the response of eelgrass (Zostera marina L.) across current temperatures to look for early warning signs of vulnerability and to evaluate the ways we determine critical thresholds for survival. Whole eelgrass ramets, collected from three beds in Morro Bay, California, …

The Effects Of Age On Muscle Loss And Tissue-Specific Levels Of Nf-ĸb And Sirt6 Proteins In Rats, Tiev C. Laguire

Master's Theses

The objective of this study was to examine the influence of age on food intake, tissue and organ mass and NF-ĸB and SIRT6 levels in various tissues. The transcription factor, Nuclear Factor Kappa-B (NF-ĸB), is associated with both catabolic and anabolic pathways of muscle metabolism and may be involved in age-related muscle loss. SIRT6 is a member of the sirtuin family of proteins that function as protein lysine deacetylases and are associated with longevity in a number of organisms. Male Sprague-Dawley rats, aged 6 months (Adult) and 21 months (Old) were fed a commercially available diet for 10-17 days. Old …

Applying Sustainability From A Systems Perspective:, Timothy Brophy

Master's Theses

Becoming sustainable is no longer an option. Current industry methods will not be able to support the necessary changes our growing society demands. In order to keep up with ever increasing demands industry will be aimed toward making major system changes toward a fully sustainable model. As of right now only basic sustainability actions have been put in place. Industry needs to make a strategic change toward resilient systems with an end goal of evolving into fully biomimic systems. This thesis covers a brief history of production leading to the Industrial Revolution which created a need for increased sustainability efforts. …

The Bicycle-Powered Smartphone Charger, Chris Arntzen

Master's Theses

This thesis entails the design and fabrication of a smartphone charger that is powered by a bicycle dynamo hub. In addition to the design and validation of the charger prototype, this thesis involves the testing and characterization of the dynamo hub power source, the design and construction of specialized test equipment, and the design and prototyping of a handlebar-mounted case for the smartphone and charging electronics. With the intention of making the device a commercial product, price, aesthetics, and marketability are of importance to the design. An appropriate description of the charger circuit is a microcontroller-based energy management system, tailored …

A Multiphysics Simulation Of A Coronary Artery, Garry L. Howe

Master's Theses

This thesis hypothesizes that a commercially available multiphysics simulation software package can be easily employed to characterize the fluid flow and mechanical behavior in healthy and diseased arteries. To this end, multiphysics model of a coronary artery —the LAD artery—was created in COMSOL Multiphysics®. Wall shear stresses and distension were examined to validate the model. Such a model can be adapted to guide the design of cardiovascular devices.

Getting Biologists To Drop Acid, Ryan Verdon

Master's Theses

Bioinformatics is the field of science in which biology, computer science, and information technology merge to form a single discipline. The ultimate goal of the field is to enable the discovery of new biological insights as well as to create a global perspective from which unifying principles in biology can be discerned. Bioinformatics involves the analysis of various types of data from multiple sources to create a model of a physical process found in the real world. To do the necessary modeling, data is being piled up in various different biological databases. So far this has worked relatively well. Unfortunately …

Comparison Of Biofilm Media In Reciprocating Biofilters Treating Dairy Flushwater, Kyle Keoki Tatsuo Fooks

Master's Theses

Reciprocating biofilters known as ReCip is a viable technology to manage nutrients, mainly nitrogen, problems at livestock operations such as swine farms and dairies. Past studies have demonstrated that ReCip is more adept at total nitrogen (TN) removal than traditional subsurface flow wetland systems. The traditional substrate used to attach biomass was rock aggregate; this media may be hard to obtain for some agricultural projects, so alternate substrates are tested and compared with the rock aggregate. The purpose of the study was twofold: first, different biofilm media were tested and compared in terms of treatment performance and, second, the long-term …

Settling Performance In Wastewater Fed High Rate Algae Ponds, Elliott Blake Ripley

Master's Theses

Although high rate algae ponds (HRAPs) are a proven wastewater treatment technology with numerous environmental, social, and economic benefits, their widespread use has been hindered by inconsistent and unreliable settling performance. Hence, the goal of this thesis is to investigate how specific operational parameters affect the settling performance of HRAPs. Nine HRAPs (30 m² surface area, 0.3 m depth) were operated as three triplicate sets, with each set run on either a 2, 3, or 4 day HRT continuously from January 25, 2012 through April 11, 2013. Settling performance was determined (i) by measuring the TSS of Imhoff cone …

Associations Between Alcohol Consumption And Fasting Blood Glucose In Young Adults, Julie Ann Lucca

Master's Theses

Current research shows moderate alcohol consumption is associated with decreased risk of diabetes and excessive consumption or binge drinking can cause insulin resistance and diabetes. In 2010, diabetes was the seventh leading cause of death in the United Statesand was responsible for significant health complications: blindness, kidney failure, and limb amputations, and is a large national economic burden. Fasting blood glucose (FBG) is a tool used to help diagnose diabetes. Abnormally high FBG, ≥100 mg/dl, is indicative of diabetes and pre-diabetes. Few studies have observed diabetic prevalence among young adults or college students. Studying young adults can help provide added …

High School Athletic Directors Perceptions Of Athletic Trainer's Professional Knowledge And Competence Based On Attire, Raechel N. Cunningham

Master's Theses

Very little research is available to identify how attire in the athletic training field impacts perceptions of professional knowledge and competence. The purpose of this study was to identify the effect of attire on high school athletic directors’ perceptions of an athletic trainer’s professional knowledge and competence. The researcher emailed a questionnaire to ninety-four high school athletic directors from the state of California. The questionnaire measured the effect of attire on the perceptions of skills, knowledge, approachability, experience, education level, overall competence, and representation of an athletic trainer and focused on the difference between khaki attire, professional attire, and workout …

Root Kinematics In Relation To Temperature And Genome Size In Wild And Domesticated Zea., Avery B. Cromwell

Master's Theses

We studied root kinematics in relation to temperature and genome size variation in teosinte (Zea mays subspecies parviglumis) and corn (Zea mays subspecies mays). Corn had significantly faster radicle growth than teosinte when grown at a constant temperature. Both species exhibited variation in seed size and for each species larger seeds had faster root growth. Genome size was not significantly correlated with faster radicle growth rates across multiple land races of corn. To examine temperature dependent growth in corn and teosinte, a germinated seedling was grown at multiple temperatures. Growth rates at these temperatures were used …

Uav Formation Flight Utilizing A Low Cost, Open Source Configuration, Christian W. Lopez

Master's Theses

The control of multiple unmanned aerial vehicles (UAVs) in a swarm or cooperative team scenario has been a topic of great interest for well over a decade, growing steadily with the advancements in UAV technologies. In the academic community, a majority of the studies conducted rely on simulation to test developed control strategies, with only a few institutions known to have nurtured the infrastructure required to propel multiple UAV control studies beyond simulation and into experimental testing. With the Cal Poly UAV FLOC Project, such an infrastructure was created, paving the way for future experimentation with multiple UAV control systems. …

Photoionization Mass Spectrometry And Photoelectron-Photoion Coincidence (Pepico) Spectroscopy Studies Of Select Biofuel Molecules, Joseph G. Czekner

Master's Theses

This thesis is the culmination of numerous experiments, performed on two different continents, investigating the spectroscopic and thermodynamic properties of several biofuels and fuel additives. It will start with an introduction about the motivation behind these experiments. The second chapter will outline the experimental details of the apparatus at the Advanced Light Source (ALS) in Berkeley, CA, followed by the Swiss Light Source (SLS) in Villigen, Switzerland. Third, the theoretical concepts and data analysis methods will be discussed in detail.

Chapter 4 will be the start of the newly obtained data. It presents some photoionization mass spectrometry studies on γ-valerolactone …