Digital Commons Network^™

Adsorption/Desorption Process Of Stimuli-Responsive Polymers On Simulated Natural Surfaces Using Qcm-D & Afm Analysis, Yan Zong

Master's Theses

The stimuli-responsive adsorption of polyelectrolytes to biosurfaces provides an important vehicle for development of protective coatings, delivery of therapeutic agents, and cosmetic applications. Developing a fundamental understanding of the mechanisms and kinetics of adsorption/desorption processes of polymeric systems to biological surfaces is of critical importance in predicting performance and designing new formulations. This study describes quartz crystal microbalance and atomic force microscopy analysis of a poly(methyl vinyl ether-alt-maleic anhydride) copolymer (Gantrez® S97 BF) adsorption on dental mimicking surfaces to determine properties including thickness, morphology, viscoelasticy, and rate of adsorption/desorption of the polymer layer as a function of solution environment. A …

Friction Ridge Dysplasia And Its Preponderance In The Afghanistan Population, Chelsea Elyse Woullard

Master's Theses

Friction Ridge Dysplasia is a rare genetic disorder in which the friction skin ridge units do not fuse together to form continuously flowing friction ridges. The skin affected by Friction Ridge Dysplasia is generally localized to one area and gives a similar appearance of the pebbled state of skin on the snout of a dog. Many authors have briefly discussed Friction Ridge Dysplasia in their publications; however, in-depth reflection as to the cause and rate of occurrence of Friction Ridge Dysplasia has not been documented. The objective of this study is to determine whether or not Friction Ridge Dysplasia is …

Sensitivity And Species Specificity Of Bisulfite Modification And Pyrosequencing Technology Used To Identify Tissue-Specific Dna Methylation Patterns In Common Biofluids Foudn In Crime Scenes, Sitara Christian Shirwani

Master's Theses

In order to convict the guilty or exonerate the innocent in criminal cases, it is crucial to reconstruct the crime scene and/or determine the nature of the crime. Identifying the different biofluids found at a crime scene can help shed light on these aspects of forensic casework. Recently, DNA methylation has been used as a means of identifying biological materials, as opposed to conventional protein/enzyme based methods. DNA methylation is the addition of a methyl group to the 5' carbon on a cytosine base (C), which is directly followed by a guanine base (G) and are called CpG sites. The …

Narratives By Six Year Old And Nine Year Old Boys: Brute: Institutional, And Non-Institutional Mental Facts, Adam Craig Whipple

Master's Theses

Brute facts, institutional facts, and non-institutional mental facts were studied. The philosophy of constructionism and the theory of intent provided a framework for this research. Intentionality provided the basis for social facts. Brute, institutional, and noninstitutional mental facts were operationally defined. This study analyzed the use of these facts in the narratives of 6-year-old boys and 9-year-old boys. There were a total of 19 participants in this research. This research established brute, institutional, and non-institutional mental facts as appropriate operational categories for studying children's narratives. The 6-year-old boys produced more brute facts than the 9-year-old boys. The 9-year-old boys produced …

Assessing Respiration Rates And Nutrient Dynamics Of Aritifical Reef Biofilms And Bacterioplankton In The Mississippi Sound, Lynn Elizabeth Wilking

Master's Theses

Artificial reefs are primarily used to provide a suitable habitat for target fish populations, but the structures can also improve water quality and benefit non-target organisms. Laboratory incubation experiments were conducted in the presence of biofilm on rubble and in its absence to examine bacterial growth, community respiration, and nutrient dynamics at four artificial reef habitats in the Mississippi Sound. Biofilm samples were also collected from settlement plates deployed at each site and were analyzed for 813C and 81sN stable isotope content. Respiration rates were always higher in the presence of biofilm but bacterial abundance often declined over time, and …

Variations Of Vibrio Populations In Pacific White Shrimp, Litopenaeus Vannamei, Aquaculture Raceways, Tracy Rene Berutti

Master's Theses

Reducing incidence of vibrioses in intensive aquaculture systems is essential to maintaining yields. This study performed real-time qPCR (quantitative polymerase chain reaction) using primers targeting 16s rDNA sequences for total bacteria, the Vibrio genus, and Vibrio parahaemolyticus in aquaculture tanks over the growing season. Total DNA concentrations in each sample were measured with a NanoDrop 2000 Spectrophotometer (Thermo Scientific, Inc. USA). These data were compared to two levels of bio-floc solids removal in addition to physical and chemical tank parameters such as temperature, ammonia, nitrite, nitrate and phosphate. The total bacteria gene copy abundances were higher (p = 0.0081) …

Seasonal And Diurnal Behavioral Patterns Of Bottlenose Dolphins, Tursiops Truncatus, That Exhibit High Site And Low Site Fidelity To Mississippi Sound, Shauna Marisa Mcbride

Master's Theses

This study examined whether bottlenose dolphins (Tursiops truncatus) exhibit site fidelity to the Mississippi Sound and how the seasonal and diurnal behavioral patterns of dolphins that exhibit high site fidelity to the Mississippi Sound differ from those of dolphins with lower site fidelity. Opportunistic surveys conducted from July 2006 to April 2010 were analyzed. Statistical analyses consisted of nonparametric tests (Spearman's correlation, loglinear models, and Pearson's chi-square) to compare behavioral patterns of high site fidelity, mixed, and low site fidelity groups. Behavioral patterns significantly differed between site fidelity groups across seasons and diurnal periods. Feeding behavior was observed significantly more …

Effect Of Antimicrobials And Sodium Replacement Agents On The Survival Of Pathogenic Bacteria In Low Sodium Low-Moisture Part-Skim (Lmps) Mozzarella Cheese, Tiffany M J Taylor

Master's Theses

Recent increases in chronic cardiovascular diseases, such as hypertension, have put pressure on the food industry to reduce sodium levels. Dairy products, though full of vital nutrients, are perceived as being high in sodium. However, the reduction of salt in dairy products could potentially alter the microbial stability, as well as cause unfavorable changes in flavor. In order to reduce the sodium level, while maintaining acceptable flavor and microbial stability, salt replacers and alternative antimicrobial agents may need to be introduced into the food matrix. To identify potential antimicrobials for use in reduced sodium dairy products, this study evaluated the …

Reassessment Of Biowish Activation Procedure For Denitrification, William Wah Yee

Master's Theses

BiOWiSH^TM – Aqua is a blend of preserved multi-bacteria culture with the capability of denitrification. If an anaerobic nitrate rich activation procedure is used instead of the standard aerobic activation procedure, the denitrification rate is increased by 28 percent under the conditions of 30°C, 1C:1N, 200mg/L of carbon, and 200mg/L nitrogen.

Changes In Soil Salinity Levels With The Use Of Recycled Water On Cool Season Vegetables, Dana Cameron Ripley

Master's Theses

Agricultural production in Monterey County, California is a multi-billion dollar industry. Near the coast, seawater intrusion has threatened to degrade the groundwater quality due to over-pumping of the aquifer. The Monterey Regional Water Pollution Control Agency (MRWPCA), in partnership with the Monterey County Water Resources Agency, has provided recycled water since 1998 to over 12,000 acres of prime agricultural farmland in the northern Salinas Valley in an effort to reduce groundwater removal. The dominant soil types in the region are clay loam and clay soils, which are both susceptible to sodium (Na) accumulation and water infiltration problems. Recycled water blended …

Lipid Production By Microalgae Treating Municipal Wastewater, James Edward Kelley

Master's Theses

Microalgae hold much promise as a feedstock in liquid biofuel production. Lipid content of microalgae cells range from 30-80% dry weight of biomass. It is projected that microalgae can produce between 1,000-6,500 gallons/acre/year of oil. Currently, production of industrial algae operates in open raceway ponds that use minimal capital and energy inputs to culture algae. Raceway ponds can also be used to grow microalgae from municipal waste streams. Although high biomass productivity can be achieved in these systems, there remains a large production gap between large volumes of biomass cultivation and high lipid content from microalgae cells. Low lipid content …

Protective Effects Of Sphingomyelin Against Uv Photodamage In Human Keratinocytes, Kathleen De Guzman

Master's Theses

Ultraviolet (UV) radiation has been demonstrated in numerous studies to be a major risk factor for non-melanoma skin cancer development. Despite the emergence of current UV-preventative strategies, such as sunscreens and skin-protective clothing, the incidence of non-melanoma skin cancer has continued to rise. This has encouraged investigations on alternative methods for UV prevention. In particular, bovine milk sphingomyelin has been studied for its potential in protecting human skin against UV photodamage. While the previous studies have suggested that sphingomyelin exhibits UV-protective properties in a human skin equivalent model, the exact mechanisms behind sphingomyelin’s photoprotective effects are yet unknown.

This thesis …

Development And Validation Of A Human Hip Joint Finite Element Model For Tissue Stress And Strain Predictions During Gait, Jeffrey D. Pyle

Master's Theses

Articular cartilage degeneration, called osteoarthritis, in the hip joint is a serious condition that affects millions of individuals yearly, with limited clinical solutions available to prevent or slow progression of damage. Additionally, the effects of high-risk factors (e.g. obesity, soft and hard tissue injuries, abnormal joint alignment, amputations) on the progression of osteoarthritis are not fully understood. Therefore, the objective of this thesis is to generate a finite element model for predicting osteochondral tissue stress and strain in the human hip joint during gait, with a future goal of using this model in clinically relevant studies aimed at prevention, treatment, …

An Assessment Of Habitat Suitability For Pronghorn Populations Of The Central Valley Region Of California, Virginia Burroughs

Master's Theses

Efforts to reintroduce and maintain populations of pronghorn (Antilocapra americana) to the California Central Valley, specifically the Carrizo Plain National Monument (CPNM) and the Mojave Desert (Antelope Valley) portion of Tejon Ranch, have largely been unsuccessful due to dwindling numbers of translocated animals. The objective of this study was to improve upon previous models for the CPNM using aerial survey data and then apply the model to the Tejon Ranch. Aerial survey data collected from 2000-2010 on the CPNM was used to establish “use” and “non-use” areas in the model. Model variables included vegetation type (forest, shrub, grassland, …

Mechanical Properties Of Bone Due To Sost Expression: Nanoindentation Assessment Of Murine Femurs, Amir Rafie

Master's Theses

In the human genome, the SOST gene codes for a protein sclerostin. Sclerostin is an osteocyte-expressed negative regulator of bone formation. When the SOST gene is not coded, bone formation is reduced in individuals during skeletal maturation. This study utilizes nanoindentation methods to test for the mechanical properties of bones that both express and do not express the SOST gene. 100 transgenic murine femurs were obtained from Lawrence Livermore Labs in the form of 6 and 8 month SOST transgenic mice, 6 and 12 month SOST knockout mice, and wild type control littermates for each of the 4 age groups. …

The Influence Of Hydrogeomorphology, Soil Redox Conditions, And Salinity On The Spatial Zoning Of Saltgrass, Salt Rush, And Cattails In Scotts Creek Marsh, Swanton Pacific Ranch, Ca, Mark D. Gormley

Master's Theses

Scotts Creek Marsh (SCM) is a small coastal wetland ecosystem in Davenport, CA. The vegetation of SCM is dominated by three halophytic zones comprised of saltgrass, salt rush, cattails. The objectives of the study were (i) to investigate the variables that influence the zoning of the three dominant halophyte communities in SCM and (ii) to the test the effectiveness of Indicator of Reduction in Soil (IRIS) tubes to indicate the reduction of S. The study examined the following parameters from April 6 to July 21, 2013: (i) the HGM of Scotts Creek Marsh, (ii) soil oxidation and reduction (redox) conditions, …

Conjugated Linoleic Acids Alter Body Composition Differently According To Physiological Age In Moulard Ducks, Jeff Fesler, Daniel Peterson

Master's Theses

Conjugated linoleic acids (CLA) have been shown to have remarkable yet inconsistent metabolic effects in mice, rats, hamsters, chickens, cattle, and humans. In particular, effects on lipogenesis vary with tissue, physiological state and specie. In this study we tested the hypothesis that CLA would differentially affect ducks of the same genetic background but of differing age. Growing (7 wk) and maintenance (11 wk) Moulard ducks were grouped by age and fed a standard diet supplemented with either 5% soybean oil (control) or 5% CLA isomer mixture. Animals were harvested after 3 weeks or 6 weeks for assessment of body composition …

Determining Parentage And The Effects Of Relatedness On Play Partner Preference In Belding's Ground Squirrels, Jessica Weidenbach

Master's Theses

I investigated 1) multiple paternity in Belding’s ground squirrels (Urocitellus beldingi) and 2) social partner preferences in juvenile U. beldingi. Prior work with blood allozymes demonstrated multiple paternity in U. beldingi litters. I evaluated paternity using microsatellite DNA analysis, which is more accurate than examining blood allozymes. My results indicate that multiple paternity in U. beldingi is more extensive than previously shown, occurring in about 90% of litters with more than one juvenile, and averaging 2.95 fathers in multiply sired litters. I also evaluated the hypothesis that play and other social behavior promotes bonding among juvenile female …

Building Women’S Solidarity To Advance Women’S Rights In Bolivia, Luzdary Hammad

Master's Theses

This paper takes a historical look at the deep-seated ethnic and class divisions between women in Bolivia. It also examines the cultural challenges that help explain the status of women in Bolivia and the obstacles women face to become politically active. It provides the theories of decolonization and depatriachalization as practical ways Bolivia can move past their colonial and patriarchal history. It also looks into what feminism means overall in Latin America and what strategies Latin American women have used to make change for women. It then provides a political history of Bolivia from 1994 to the present giving the …

Women And The Second Estate In 16th Century Zambezia: Gendered Powers, A 'Puppet' African Queen And Succession In Vakaranga Society, 1500–1700, George G. Levin

Master's Theses

Women in vaKaranga society of the 15th to 17th centuries have been portrayed as oppressed by an "extremely patriarchal" system, but the reality, while still fitting the simple classification of a 'patriarchal' monarchy, indicates quite a bit more negotiation of gendered powers than women, as a class, experienced in the Mediterranean or East Asia. The vaKaranga were the architects of Great Zimbabwe, the capital of a growing state, colonizing their cousins of the Zambezi river, which their Kusi-Mashariki Bantu forefathers had traversed southward a millennium before. Civil war had (apparently) split one nation into two states, Mutapa (Monomotapa) and Khami …

Coalescence Kinetics Of Lipid Based Bicelles, Andrew Hu

Master's Theses

Uniform nanodisc structures can be self assembled from mixtures of different phospholipids. This study focuses on the theoretical and experimental growth kinetics of phospholipid based nanodiscs. Motivation for this project comes from the nanodisc’s small size and their potential use as a carrier for drug delivery. It was observed that at high total lipid concentration the nanodiscs are stable at approximately 10 nm in radius and can remain stable for a long period of time. However, growth of these nanodiscs are observed at relatively low total lipid concentration. The observed growth mechanism is not well understood. In this thesis dynamic …

Identifying Chromosome Rearrangements In The Allopolyploid Brassica Napus Using Pyrosequencing, Alexandra R. Barbella

Master's Theses

Allopolyploids form through the hybridization of two or more diploid genomes. A challenge to reproduction in allopolyploids is that pairing can occur between homologous chromosomes or homeologous chromosomes (i.e.different subgenomes.). Crossover between homeologous chromosomes can result in chromosome rearrangements that lower fertility and overall fitness. Rearrangements can alter the dosage of either entire chromosomes or just parts of chromosomes. Understanding the frequency and extent of rearrangements will help to explain the evolution and genome stabilization of agriculturally important allopolyploid species. Pyrosequencing is a useful tool in the study dosage changes in allopolyploids because it allows quantification of the relative contribution …

Effects Of Racism And Discrimination On Personality Development Among African American Male Repeat Offenders, Tiffany Nicole Lockett

Master's Theses

ABSTRACT

Effects of Racism and Discrimination on Personality Development among

African American Male Repeat Offenders

Tiffany Nicole Lockett

Throughout history in the United States, the African American community has consistently been the victim of social policies put in place to disenfranchise this population (Mauer & Chesney-Lind, 2002; Painter, 2007; Parham, White & Ajamu, 1999). With a longstanding presence of systemic racism and discrimination, the criminal justice system and the dominant culture continues to pathologize this minority group and advocate for increased penalties which further stigmatize African Americans, particularly males in this group (Reiman, 1996; Russell, 1998). Though most criminology research …

An Examination Of Marine Snow As A Pathway For Mercury Uptake In The Blue Mussel Mytilus Edulis, Veronica L. Ortiz

Master's Theses

Bioaccumulation of mercury (Hg), especially as methylmercury (CH₃Hg), in the aquatic food web is a continuing public health concern. Extensive research has been conducted regarding the uptake of Hg by various aquatic animals via three main vectors: sediment, bioseston, and water, but virtually no research exists with respect to uptake of CH₃Hgor inorganic mercury (Hg(II)) associated with marine snow. Marine snow composed of various organic and inorganic materials has been studied as an effective vertical transporter of nutrients and metals scavenged from the euphotic zone to the benthos. As various aquatic species utilize marine aggregates as …

Analysis And Comparison Of Parallel Plate Flow Chambers To Determine Consistency Of Fluid Forces On Cells, Sampat S. Nidadavolu

Master's Theses

Biological cells are constantly exposed to fluid forces inside the body. These fluid forces aid in certain physical and chemical reactions that cells need to maintain physiological function. To observe these forces in vitro, parallel plate flow chambers (PPFC) are used, where cells are placed inside the chamber and a fluid medium runs through the device exposing the cells to fluid forces to initiate a response. This has aided in proving that fluid forces influence cell function and are factors in various disease and physiological processes, such as the development of atherosclerotic plaque in blood vessels or bone growth. Many …

The Effects Of Poor Maternal Nutrition On Postnatal Growth And Development Of Lambs, Kristen N. Peck

Master's Theses

No abstract provided.

Fabrication And Characterization Of A Palladium/Porous Silicon Layer, Nicholas Hong Lui

Master's Theses

When porous silicon is plated with a catalytic metal, the two materials can act together as a single entity whose electrical properties are sensitive to its environment – the sensing component of an electrochemical gas sensor. Etching pores into silicon is an electrochemical process; and which type of doped silicon used is one of its key parameters. For nearly all reported porous silicon gas sensors, the silicon has been of the p-doped variety – because p-doped porous etching is better understood and the layers that result from it are more predictable – despite n-doped silicon having potentially significant benefits in …

An Analysis Of Eliminating Electroosmotic Flow In A Microfluidic Pdms Chip, Cecile D. Redington

Master's Theses

The goal of this project is to eliminate electroosmotic flow (EOF) in a microfluidic chip. EOF is a naturally occurring phenomenon at the fluid-surface interface in microfluidic chips when an electric field is applied across the fluid. When isoelectric focusing (IEF) is carried out to separate proteins based on their surface charge, the analytes must remain in the separation chamber, and not migrate to adjacent features in the microfluidic chip, which happens with EOF.

For this project, a microfluidic chip was designed and commissioned to be photolithographically transferred onto a Si wafer. A PDMS component was then casted on the …

Exploring The Solution Behavior Of The Translational Gtpase Bipa Using Molecular Dynamics, Gilman L. Dionne

Master's Theses

BipA is a conserved prokaryotic GTPase necessary for securing bacterial survival and successful invasion of the host. Structural and biochemical studies indicate that GTP and ppGpp compete for binding to BipA to promote differential association of BipA to either the 70S or 30S ribosomal species. Exactly how guanine nucleotide binding to BipA prompts a change in the association of this protein with the ribosome is not understood. Crystallographic models show local structural rearrangements occur near the nucleotide-binding pocket but unexpectedly the overall domain arrangement, and therefore intramolecular contacts are similar in the various guanine nucleotide bound states. One explanation is …

Comparative Gene Analysis And Prediction Of Innate Immunity And Apoptosis Machinery In Hydrothermal Vent Tubeworms, Corey M. Bunce

Master's Theses

Siboglinid tubeworms are an emerging model system for symbiosis in extreme conditions. At deep-sea hydrothermal vents in the Pacific Ocean, they harbor a chemoautotrophic symbiont in a specialized organ, the trophosome, where the bacteria carry out primary production via oxidation of reduced sulfur compounds. The trophosome of siboglinid worms is suggested to be tightly regulated, symbiont-responsive, and developmentally homeostatic. The purported mechanism involves a cellular proliferative or apoptotic response to bacterial signals, environmental cues, or nutrition. Though these processes are well understood in more amenable model systems, there has been little work uncovering the tubeworm’s genetic potential for sensing and …