Digital Commons Network^™

The 2013 Boston Marathon Attacks: An Experimental Investigation Of Worldview Change Following Film Re-Exposure To Mass Trauma, Ian A. Gutierrez

Master's Theses

Models of meaning making and post-traumatic growth contend that traumatic events are catalysts for significant and long-lasting change in a person’s worldview—that is, their beliefs about social, metaphysical, theological, and political reality. Nearly all tests of this contention have employed retrospective and cross-sectional methods. However, the limitations inherent in these designs preclude causal conclusions. The current study represents the first longitudinal, laboratory-based experimental examination of belief change following trauma exposure using the trauma film paradigm. Findings reveal that the trauma film paradigm can successfully induce posttraumatic affective responses with live news coverage of a mass trauma (i.e., the 2013 Boston …

The Impact Of A Smarter Lunchrooms Makeover On Children's Food Choice And Consumption, Polina Zhuzhina

Master's Theses

Childhood obesity has tripled over the past three decades and poses a serious public health problem. The Smarter Lunchrooms Movement aims to increase healthy eating by incorporating low-cost to no-cost alterations to the school lunchroom in an effort to increase consumption of healthier foods, such as fruits and vegetables, by making them more attractive and convenient.

Our study implemented Smarter Lunchrooms interventions at two primarily Hispanic elementary schools in Paso Robles, CA. The interventions focused on increasing the appeal of the salad bars, including installation of age-appropriate signage highlighting fruits and vegetables, as well as branding of fruits and vegetables …

The Use Of Lactic Acid Bacteria To Control The Growth Of Foodborne Pathogens On Fresh-Cut Fruits And Sprout Vegetables, Franca Gabriela Rossi

Master's Theses

Growing consumer awareness of the health benefits associated with fruits and vegetables and demand for easy to prepare products has prompted the development of a wide variety of minimally processed fruits and vegetables. Minimally processed fruits and vegetables are often peeled, cut, or diced which compromise the produces’ natural protective barriers, exposing a nutrient rich medium and providing an ideal environment for the growth of microorganisms, including foodborne pathogens. The germination conditions of sprout vegetables consisting of relatively high temperatures and humidity, low light and abundance of nutrients are also conducive to the proliferation of foodborne pathogens. Recent outbreaks and …

Evaluating The Myth Of Allelopathy In California Blue Gum Plantations, Kristen Marie Nelson

Master's Theses

It is widely accepted that allelopathy is not only significant, but more or less singular, in the inhibition of understory vegetation in California Eucalyptus globulus (blue gum) plantations. However, there is no published documentation of allelopathy by blue gums against California native species. Here, we present evidence that germination and early seedling growth of five California native species are not inhibited by chemical extracts of blue gum foliage, either at naturally-occurring or artificially concentrated levels. In the greenhouse, seeds were germinated in field-collected soil from mature blue gum plantations and the adjacent native, coastal scrub communities. In petri plates, seeds …

Regrowth Of Chlorella Sorokiniana On Recycled Media With Replenished Nutrients, William H. Spence

Master's Theses

Growth media recycling during algae cultivation is necessary to increase the efficiency and reduce the cost of biofuel production from algae feedstocks. Without recycling media, the cost of algae based biofuel production would be prohibitively high and large scale algae based biofuel production would not be economically viable. The ratio of media recycled to media wasted assumed for algae farms is generally calculated to maintain salt concentrations below growth inhibitory levels, ignoring the influence of secondary metabolites which might decrease productivity. Secondary metabolites, which include allelopathic or auto-inhibitory biological contaminants, might lead to the accumulation of growth-inhibiting compounds in recycled …

Influence Of Chelating Agents On Proteolysis Of Micellar Casein Slurries, Azucena Becerril Diez

Master's Theses

ABSTRACT

Influence of chelating agents on proteolysis

of micellar casein slurries

Azucena Becerril Díez

The focus of this research was to analyze the effect of Ca chelators on the proteolytic activity of rennet during ripening of a micellar casein slurry. This research was divided in two phases. During the first phase, preliminary studies were performed to understand the behavior of rennet and the milk system in the presence of chelating agents. A solution of reconstituted skim milk at 10% (w/v) was prepared. Individual samples of this solution were divided in two study groups, samples with chelators and rennet and samples …

Evaluation Of The Effects Of Walnut Extract On The Cell Division G2/M Cyclin-Cyclin B1 In Breast Cancer Cells, Savita Pinaki Shah

Master's Theses

Walnuts are rich in polyphenols and have potential for cancer prevention and treatment. Our lab has previously shown that a walnut extract (WE) is able to block the cell cycle at the S and G2 phases of cell division with a corresponding decrease in the amount of the cyclin B1 protein and induce cell death in human breast cancer cells (Le et al., 2014). The aim of the current research was to identify whether this effect on the expression of cyclin B1 was at the transcriptional or the translational level. A cycloheximide (CHX) chase revealed that a WE does not …

A Sedimentological Analysis Of The Siliciclastic Fraction In Pliocene Core Sediments From Bowers Ridge, Bering Sea (Iodp Exp 323), Melinda Tanner

Master's Theses

The analysis and interpretation of sediment cores constitutes an important data record that can be used to evaluate paleoclimatic conditions and changes over time. The causes for the cooling trend that began in the Eocene, and its intensification with the onset of North Hemisphere Glaciation (NHG), remain under debate. Using sediment core samples from Site U1341 in the Bering Sea, the principal goal of this thesis was to examine the record at 1.9-4.2 Ma, using sedimentologic tools to interpret the siliciclastic fraction and ascertain whether and which portion is due to ice-related input. Previous research estimates the onset to begin …

Cultural Influences In Political Ideology: Conservatism And Vertical Individualism, Cyrus Sarrafpour

Master's Theses

After an extensive literature review on authoritarianism and right-wing ideology, Jost, Kruglanski, Glaser, and Sulloway (2003) developed a model of conservatism as motivated social cognition. The model asserts that feelings of uncertainty, fear, and threat stimulate existential, epistemic, and ideological social-cognitive motives, which in turn lead to political conservatism in the forms of resistance to change and endorsement of inequality. The model, however, generally bypassed the notion that situational factors, such as cultural values, might also be influential in the manifestation and shaping of conservative ideologies. This study included situational factors such as the role of a hierarchical cultural dimension …

Evaluation Of The Estrogenic And Osmoregulatory Impacts Of Exposure To 4-Nonylphenol Pollution In The Estuarine Arrow Goby, Clevelandia Ios, Kaitlin Marie Johnson

Master's Theses

Recent evidence indicates that some of California’s coastal estuaries are contaminated with the chemical 4-nonylphenol (4-NP). Tissue burdens of 4-NP detected in the intertidal arrow goby (Clevelandia ios) in California are among the highest recorded worldwide, however, it remains unknown whether these fish are impacted by this 4-NP contamination. 4-NP is an established endocrine disrupting compound with estrogenic properties that can alter reproductive function. Furthermore, evidence that estrogens can modulate iono- and osmo-regulatory function in fishes implies that estuarine fishes exposed to 4-NP may also experience an impaired ability to maintain hydromineral balance. In Chapter 1 of this …

Stress Ecology Of The Pacific Rattlesnakes (Crotalus Oreganus And Crotalus Helleri), Natalie Claunch

Master's Theses

Stress is a physiological state induced by disturbance or adverse environmental conditions and is modulated by the glucocorticoid hormone corticosterone (CORT) in reptiles. Stressors can have various impacts on vertebrate trait expression and may affect survival or reproduction. Little is known about the effects of chronically elevated CORT in free-ranging reptiles, or the effect of disturbance stress on venom composition in captive snakes.

In chapter 1, we investigated the effects of researcher induced disturbance on CORT levels and venom composition in a group of captive Northern Pacific rattlesnakes (Crotalus oreganus). Venom protein concentration and plasma CORT levels were …

Effects Of Bisphenol-A And Styrene On Fertilization And Development Of The Purple Sea Urchin (Strongylocentrotus Purpuratus), Nicole C. Uibel, Nikki L. Adams, Jennifer Carroll, Sean Lema

Master's Theses

Plastic waste and debris have greatly increased in the marine environment during the past 50 years. Not only do these plastics entangle and get ingested by marine mammals, turtles, and sea birds, but they also leach chemicals, such as bisphenol-A (BPA) and styrene into the aquatic environment. While some of these chemicals are known to be toxic, few studies have examined their effects on broadcast spawning marine invertebrates, specifically at environmentally relevant concentrations. Purple sea urchins, Strongylocentrotus purpuratus, from the central coast of California, were utilized to examine effects of BPA and styrene on fertilization success and early development of …

Genes Encoding Flower- And Root-Specific Functions Are More Resistant To Fractionation Than Globally Expressed Genes In Brassica Rapa, Naiyerah F. Kolkailah

Master's Theses

Like many angiosperms, Brassica rapa underwent several rounds of whole genome duplication during its evolutionary history. Brassica rapa is particularly valuable for studying genome evolution because it also experienced whole genome triplication shortly after it diverged from the common ancestor it shares with Arabidopsis thaliana about 17-20 million years ago. While many B. rapa genes appear resistant to paralog retention, close to 50% of B. rapa genes have retained multiple, paralogous loci for millions of years and appear to be multi-copy tolerant. Based on previous studies, gene function may contribute to the selective pressure driving certain genes back to singleton …

The Effects Of Quorum Sensing And Temperature On The Soluble Proteome Of Vibrio Salmonicida, Christopher L. Massey

Master's Theses

Vibrio salmonicida causes cold-water vibriosis in salmon populations around the world and causes financial damage to fisheries designed to farm these salmon. Very little is known about the physiology of how V. salmonicida causes disease and measures to contain vibriosis are restricted to either vaccinating individual fish against disease or administering antibiotics when an outbreak is detected. These procedures are costly and increase the risk for selection of antibiotic-resistant V. salmonicida strains. A recent reoccurrence of outbreaks in Norwegian fisheries provided incentive to better understand the virulence mechanisms of V. salmonicida. In this thesis, a proteomic approach was used to …

The Influence Of Exclusive Breastfeeding On Growth Trajectory From Birth To Year 5 In A Wic Cohort, Lindsey Arevalos

Master's Theses

OBJECTIVE. The impact of exclusively breastfeeding (EBF) on child body mass index (BMI) from birth through 60 months of age was investigated.

METHODS. 60,190 mothers and children attending Women, Infant and Children (WIC) clinics in the Santa Barbara and San Luis Obispo counties of California provided data on breastfeeding duration, maternal pre-pregnancy BMI, and child weight-for-length (WL) was measured at 3 month intervals until 5 years of age. Missing time points were interpolated in determining child BMI/WL z-score trajectories plotted on the Center of Disease Control (CDC) infant and child growth curves. Multivariate analysis of variance was used to contrast …

An Ergonomics Analysis Of Redundancy Effect In Touch Screen Design For The Aged Population, Sarah Riesenberg

Master's Theses

Touch screen technology is rapidly increasing, and at the same time there is a shifting, aging population. As the percentage of the population over the age of 65 increases, adults of the age group are adopting smartphones and tablets more now than ever before. Although older adults are adopting touch screen devices, they face many challenges when interacting with said devices, such as not knowing how to navigate between pages, not knowing where to click for an action to occur, and the touch screen interface is often too sensitive or the buttons are not big enough. Furthermore, the challenges of …

Skewer: Sentiment Knowledge Extraction With Entity Recognition, Christopher James Wu

Master's Theses

The California state legislature introduces approximately 5,000 new bills each legislative session. While the legislative hearings are recorded on video, the recordings are not easily accessible to the public. The lack of official transcripts or summaries also increases the effort required to gain meaningful insight from those recordings. Therefore, the news media and the general population are largely oblivious to what transpires during legislative sessions.

Digital Democracy, a project started by the Cal Poly Institute for Advanced Technology and Public Policy, is an online platform created to bring transparency to the California legislature. It features a searchable database of state …

Rearing Temperature Affects The Expression Of Proteins In The Adhesive Of The Striped Acorn Barnacle, Balanus Amphitrite, Melissa J. Daugherty

Master's Theses

Barnacles are dominant hard–fouling organisms in marine waters. They attach to substrates by secreting a complex proteinaceous adhesive. Understanding the chemical composition of this multi–protein underwater adhesive and how it is affected by environmental variables, such as oceanic temperatures, is critical for developing nontoxic solutions to control biofouling. Previous experiments in our lab revealed an inverse relationship between critical removal stress (CRS) and temperatures at which barnacles were reared. Further investigations showed that this correlation is not attributed to differences in physical properties such as barnacle size or short–term changes in the viscosity of adhesive. Therefore, the observed effects may …

Characterizing Neurotransmitter Receptor Activation With A Perturbation Based Decomposition Method, Stephen Gregory Jue

Master's Theses

The characterization of postsynaptic potentials, in terms of neurotransmitter receptor activation, is of clinical significance because information associated with receptor activation can be used in the diagnosis and study of neurological disorders. Single-unit recordings provide a method of measuring postsynaptic potentials in neurons using a microelectrode system, but yield no detailed information regarding the neurotransmitter receptors that contribute to the potential. To determine the types of neurotransmitter receptors that result in a compound postsynaptic potential from a microelectrode reading, decomposition of the potential is necessary. In this work, a perturbation-based decomposition method developed by R. Szlavik is evaluated for this …

Lower Scotts Creek Floodplain And Habitat Enhancement Project, Benjmain O. Cook

Master's Theses

Scotts Creek, located in northern Santa Cruz County, maintains the southernmost persistent population of Central California Coast (CCC) Coho Salmon (endangered) in addition to CCC steelhead (threatened). Fisheries biologists believe overwinter mortality due to lack of refuge habitat is the primary factor limiting salmonid production. Instream rearing habitat may also be limiting, especially during drought years. The legacy effects of historic land use practices, including dredging, wood removal, and the construction of levees, continued to limit refuge and rearing opportunities. A restoration project was implemented to improve refuge and rearing opportunities for salmonids along lower Scotts Creek by removing portions …

The Role Of Bed Shear Stress In Sediment Sorting Patterns In A Reconstructed, Gravel Bed River, Samuel Emerson

Master's Theses

The role of bed shear stress in bed surface grain size sorting was investigated on a reconstructed reach of the Merced River in the Central Valley of California. Pebble count data were collected at the inside, middle, and outside of ten bends in April 2015 and compared to data from pebble counts conducted in previous years. Output from a previously developed 2D flow model (FaSTMECH) was compared to critical shear stresses calculated from median grain-size data. Comparison of pebble count results from 2002 through 2015 showed that there was no temporally consistent pattern of coarsening or fining along the study …

Variation In Adrenal And Thyroid Hormones With Life-History Stage In Juvenile Northern Elephant Seals (Mirounga Angustirostris), Jennifer Jelincic

Master's Theses

Interpretation of stress responses in wildlife is inadequate due to the range of natural variation and potential confounds of individual and life-history variables. In marine mammals, endocrine response data are sparse and variable across species. Blood adrenal and thyroid hormones were measured in 144 chemically immobilized yearling elephant seals at Año Nuevo State Reserve to characterize variation between sexes and across semiannual haul-outs. There was no relationship between hormone concentration and time needed for collecting blood or diel pattern, suggesting that concentrations represented baseline values. Serum cortisol concentrations did not vary with gender or across fasts but increased dramatically during …

Intercolony Comparison Of Diets Of Western Gulls In Central California, Anne L. Cassell

Master's Theses

As human populations expand, they force free-ranging animals to adapt to an increasingly urban environment, resulting in changes in diets, reproductive success, and mortality. The diets of two western gull (Larus occidentalis) breeding populations in central California were compared. One colony, Año Nuevo Island (ANI), is 1 km from shore and within 30 km of a municipal landfill. The other colony, Southeast Farallon Island (SEFI), is located 45 km off the shore of San Francisco, CA. Given the proximity of ANI to the shore and the landfill, I predicted that gulls from ANI would have more garbage in their diets. …

Effects Of Global Change On Algal Biomineralization And Benthic Community Interactions On California's Temperate Rocky Reefs, Emily Marie Donham

Master's Theses

Marine ecosystems are threatened by CO2-driven global change, such as ocean warming and acidification (OA). The primary objectives of this study were to: 1) assess the response of the coralline red alga, Calliarthron cheilosporioides, to global change; and 2) investigate the responses of California’s kelp forest communities to OA and sea urchin grazing. Results indicated that C. cheilosporioides growth and calcification were reduced under both increased temperatures and increased pCO2 despite increased photosynthetic rates in high pCO2 conditions. Mineralogy did not differ among treatments as a function of warming or acidification. Differences were observed in Mg incorporation into calcified walls …

Effects Of Substrate Warming On Sessile Marine Invertebrate Communities In Monterey Bay, California, Stephen Loiacono

Master's Theses

Increasing average global sea temperatures are one of the most direct and alarming consequences of climate change, yet it is still unclear how sessile marine communities will respond in the near- and long-term. In addition, it is unclear if warming ocean temperatures will facilitate marine invasions or primarily benefit native species. To simulate the effects of increasing ocean temperatures on sessile communities, heated settlement plates (n=7) were used in an in situ field experiment to investigate how an increase in substrate temperature (2.5°C above ambient) would affect recruitment and community development as compared to unheated control plates (n=9). Experimental treatments …

Treatment Of Sk-Mel-28 Cells With R-Moj-Dm Upregulates Pro-Apoptotic Genes P53 And Tnf-B While Downregulating Oncogene C-Myc, Melissa Lombardo

Master's Theses

Cell death by apoptosis is the result of one or multiple signaling pathways that can be initiated inside or outside the cell for a variety of reasons. Integrins, expressing themselves on the cell surface, play a central role in some apoptotic pathways. Cancer cells have been known to bypass this function and upregulate survival factor genes in order to elude apoptosis and thrive in the body, thus creating an environment in which it is possible for them to grow and metastasize. Previously in Dr. Soto’s lab, a recombinant disintegrin mutant, r-Moj-DM, was shown to induce apoptosis in melanoma cell line …

Climate Variability Influences Women’S Attitudes Towards Intimate Partner Violence (Ipv), Jean Lee

Master's Theses

The World Health Organization (WHO) estimates 1 in 3 women worldwide has experienced violence against women. Our study examines the effects of climate conditions on women’s attitudes towards wife beating. The demographic health surveys (DHS) dataset includes over 550,000 women in 38 countries, asked a series of wife beating justification questions in the domestic violence module. Using a linear probability model, we find prior year growing degree days (GDD), prior and current year rainfall variability have statistically significant effects. Most interestingly, we find that a one standard deviation (SD) rise in prior year annual rainfall decreases the likelihood of justifying …

Air Pollution, Temperature And Cognitive Performance In The Short Run: Evidence From Women’S Ability To Recall Dates, Ke Yang

Master's Theses

Abstract: Cognitive performance is important to productivity across many fields and potentially correlated to air pollution and extreme temperatures. We study the effects of daily ambient air pollution and monthly temperature on women’s ability of recalling dates across 42 developing countries from 1997 to 2009. We use an estimated natural air pollution data, and calculate the AQI to get an aggregate effect of air pollution. We find that one standard deviation increase in the AQI leads to a statistical decrease in women’s probability to recall dates such as birthdays, marriage date or children’s birthdays by 0.44 percentage point. Furthermore, there …

Air Pollution And Fetal Loss In The Global Tropics, Shuqiao Cheng

Master's Theses

Abstract: Recent work has shown that in-utero air pollution has negative effects on both contemporaneous birth outcomes and long-term human capital outcomes. However, only a few studies explore the effects of in-utero exposure to air pollution on fetal loss, and none of the studies has been done in developing countries. In this study, we examine the impact of naturally caused CO and PM 2.5 on reproductive outcomes in 40 developing countries from 1997 to 2009. We present childbirths and birth gender as measures for potential fetal losses. The richest model identified using variation in pollution between pregnancies with controlling for …

Dendrimer Modified Silica Nanoparticles As Fluorescent Chemosensors For The Detection Of Copper And Cyanide Ions, Alicia Luhrs

Master's Theses

A series of silica nanoparticle-based fluorescent chemosensors were synthesized for the sensitive and selective detection of Cu²⁺ ions in aqueous solution and subsequent CN^- ion detection. Silica nanoparticles (SNP) were modified with branching polyamidoamine (PAMAM) dendrimers, which function as selective turn-off Cu²⁺ ion chelators and platforms for the attachment of rhodamine isothiocyanate (RITC) or fluorescein isothiocyanate (FITC) fluorescent dyes. In the presence of Cu²⁺ ions, the emission of the fluorescent dye is quenched by Forster resonance energy transfer (FRET) and is dependent upon dendrimer generation, pH, fluorescent dye, and surface charge. The quenching effectiveness was analyzed …