Digital Commons Network^™

A Sociofunctional Approach To Understanding Transphobia, Linas Mitchell

Master's Theses

Despite widespread evidence of anti-transgender prejudice and discrimination, research has yet to determine the nature of prejudice against transgender people. This study used the sociofunctional threat approach (Cottrell & Neuberg, 2005) to examine threat perceptions (contamination threats, obstacle threats, physical safety threats, and threats to reciprocity relations) and emotional reactions (disgust, anger, fear, pity) to transgender (transgender women, transgender men, and nonbinary people) and cisgender (cisgender women, cisgender men, and bisexual people) targets. Results from an online survey suggest that transgender targets evoke higher threat perceptions and negative emotions than cisgender targets; additionally, this tended to be the case for …

Queer People Navigating Experiences With Health Care Providers And Contraception, Dana Lavergne

Master's Theses

Contemporary views of contraception have intrinsically linked birth control to heterosexual sex and pregnancy prevention. As such, contraception is culturally understood to be exclusively for heterosexual women. Despite this, the little work that has been done on queer people1 and contraception use demonstrates they are also accessing birth control (Chrisler, Gorman, Manion, Murgo, Adams-Clark, Newton and McGrath 2015). This schism between the cultural understanding of contraception as a manifestation of heterosexual womanhood and the everyday use of contraception by both queer and heterosexual people takes root in the medical system. Based in heteronormative ideologies, the medical system fails to take …

Immunobiology Of Adenovirus-Vector Vaccines For Mrsa, Emily Orvis

Master's Theses

Staphylococcus aureus is a gram-positive, extracellular bacterium that has emerged as an

important human pathogen. This bacterium is a leading cause of skin and soft tissue infections

(SSTIs) in humans, often leading to invasive and life-threatening infections. Treatment of S.

aureus infections is becoming more complicated due to the rise of methicillin-resistant S. aureus

(MRSA) strains, which are becoming increasingly resistant to a number of antibiotics. In the

United States, invasive MRSA infections result in more deaths annually than any other infectious

agent.

Despite a dire need, there is currently no vaccine against S. aureus infections. The failure

of past …

Participation In Medical Research: Reasons Provided In Cognitive Interviews Of A Diverse Sample, Silvia Valadez

Master's Theses

Racial and ethnic minority groups are underrepresented in medical and health-related survey research, with implications for the generalizability across diverse populations of evidence gleaned from these studies. However, there is little known about the respondents’ reasons for participating—or not—in medical research studies, and how these reasons might vary across race/ethnicity, age, gender and education. In this thesis, I extend previous research by looking at data collected from cognitive interviewing techniques to examine 1) participants’ reported likelihood of participating in five increasingly invasive types of data collection, including research studies that ask participants to answer questions about themselves or provide samples …

Chironomidae (Diptera) Community Structure In Lakes Of Contrasting Morphometry, Landscape Position, And Water Chemistry, Conrad Stanley Zack

Master's Theses

Chironomidae (Diptera) surface-floating pupal exuviae were collected monthly during the ice-free season in 2010-2011 from six lakes in the North Temperate Lakes Long-Term Ecological Research site in northern Wisconsin. The goal of this study was to determine whether chironomid community structure reflected differences in lake morphometry (i.e., depth, area, shoreline development, etc.), water chemistry, and/or landscape position (i.e., elevation). Forty-six genera were identified from four subfamilies: Chironominae (57%), Orthocladiinae (28%), Tanypodinae (11%), and Diamesinae (4%). Tanytarsus, Ablabesmyia, and Psectrocladius were found in all six study lakes; whereas certain genera, such as Omisus, Protanypus, and Epoicocladius were each observed in only …

Investigating Autophagy, Extracellular Vesicles, And Glycobiology, Benjamin Cook

Master's Theses

Autophagy is a cellular process of degradation which degrades cellular materials in acidic organelles called lysosomes. Impairment of lysosomal degradation can lead to lysosomal storage disorders such as Parkinson's Disease. In Parkinson's Disease neurotoxic α-synuclein can spread from cell-to-cell via extracellular vesicles. We investigated the changes in extra vesicular glycans upon perturbation of autophagy using a class of glycan binding molecules termed lectins.

We found that upon induction of autophagy causes EV glycans to increase while others remained consistent. Additionally, we found that specific glycans are increased more than others. Conversely, when lysosomal degradation was impaired, we observed a decrease …

Induction Of Zika Virus-Specific Cd8+ T Cells By Means Of Dna Vaccine, Frank Gambino Jr.

Master's Theses

Zika virus (ZIKV) is a member of the virus family flaviviridae and is transmitted via Aedes aegypti mosquitos. Monoclonal antibodies against dengue virus have been found to cross-react with ZIKV but show no ability to neutralize it. Further studies have shown that Stat2-/- mice given plasma from dengue positive donors exhibit a heightened disease phenotype when challenged with ZIKV. Antibody dependent enhancement is theorized to cause such effect. The goal of this study was to design a plasmid which, when encoded, creates an antigen that will be used to stimulate exclusively CD8+ T cells. The gene sequence of NS3 was …

Cell Intrinsic Characteristics Of Cord Blood Naive Cd4 T Cells, Taylor Keller

Master's Theses

The neonatal immune system is functionally distinct from the adult immune system. Neonatal immune responses are less reactive than their adult counterparts, and as such, have an increased susceptibility to infection, resulting in increased duration and severity of disease. There is evidence that T-cells are partially responsible for the observed immunological differences between neonates and adults. Murine studies on neonatal immunity suggest that Th2 like responses predominate the neonatal immune system, an observation not found in the adult immune system. However, studies on human cord blood revealed that human neonates do not exhibit elevated Th2 like cytokines (with the exception …

Inhibition Of Mutant Egfr In Nsclc Promotes Endothelin-1-Mediated Nsclc Disease Progression, Stephen Ollosi

Master's Theses

Angiogenesis in NSCLC has been identified as important therapeutic target in combination with EGFR TKIs. However, only small incremental advancements have been made for the use of angiogenesis inhibitors in NSCLC and it remains elusive why the inhibition of VEGF-mediated neovascularization is not therapeutically efficacious. I present experimental evidence that a subpopulation of NSCLC cells with EGFR TKI-induced EMT contributes toward the attenuation of the response to EGFR TKI therapy. One of the hallmarks of cancer is heterogeneity and I have previously demonstrated that tumor heterogeneity within NSCLC cells lines harboring EGFR kinase domain mutations gives rise to divergent resistance …

Mechanistic Investigations Of The Iron(Iii)-Catalyzed Carbonyl-Olefin Metathesis Reaction, Susan Phan

Master's Theses

Iron(III)-catalyzed carbonyl-olefin ring-closing metathesis represents a new approach toward the assembly of molecules traditionally generated by olefinˆ’olefin metathesis or olefination. Herein, we report detailed synthetic, spectroscopic, kinetic, and computational studies to determine the mechanistic features imparted by iron(III), substrate, and temperature to the catalytic cycle. These data are consistent with an iron(III)-mediated asynchronous, concerted [2+2]-cycloaddition to form an intermediate oxetane as the turnover-limiting step. Fragmentation of the oxetane via Lewis acid-activation results in the formation of five- and six-membered unsaturated carbocycles.

Cryptic Diversification Of Two Widespread Species In Madagascar, Lynika Sharlice Strozier

Master's Theses

Using phylogenetic and phylogeographic tools to uncover hidden diversity within the genus Newtonia in Madagascar

Madagascar is known for its rich biodiversity and high level of endemic species that are found nowhere else. Cryptic diversification, defined as genetically and evolutionarily distinct species that are hard to detect because they are morphologically indistinguishable from their closest relatives, has been hypothesized to occur in many groups on Madagascar. Currently it is unclear to what extent this phenomenon occurs in birds because only a few studies have been conducted. My study examined the phylogenetic and phylogeographic patterns within a songbird genus, Newtonia, that …

International Student Intersectionality In The Trump Era, Sarah Tolman

Master's Theses

This study examines the impact of the current U.S. presidential administration's policies and rhetoric on international student experiences and identities. The researcher approaches this question through the theoretical lens of intersectionality, with the goal of highlighting the complexity and diversity of international student identities in this unique historical moment. Through in-depth narratives collected from interviews with 8 diverse international students, it becomes clear that the 2016 presidential election and the administration's rhetoric and policies have influenced international student experiences and identities in significant ways. Students perceive the statements and actions of the current government to have shifted their perspectives and …

The Characterization Of Rna Content And Biogenesis Pathways Of Extracellular Vesicles That Have Been Implicated In The Pathogenesis Of Hiv-1 Associated Neurocognitive Disorders, Virginia Elizabeth Zwikelmaier

Master's Theses

Patients infected with HIV-1 adhering to Anti-Retroviral Therapy (ART) still suffer from a variety of neurocognitive, behavioral, and motor dysfunctions, collectively referred to has HIV-1 Associated Neurocognitive Disorders (HAND). Current research suggests the manifestations of HAND might in part be due to the dissemination of toxic HIV-1 components throughout the brain via extracellular vesicles (EVs). Our goal is to characterize these pathogenic EVs to begin to gain a better understanding of this EV-mediated pathology.

We took the first steps in developing a technique to assess the microRNA content of individual EVs but it needs to be heavily optimized to eliminate …

Lidocaine Attenuates An Induced Inflammatory Response, Stephanie Zack

Master's Theses

Inflammation is common to the pathogenesis of a variety of diseases. Therapeutics which can regulate and reduce damaging inflammation are therefore valuable in the treatment of inflammation and inflammatory diseases.

One commonly used analgesic, recently identified as limiting inflammation, is lidocaine. Additionally, alternative, less toxic therapeutics like the snake venom peptides, Zep 3 and Zep 4, are thought to have anti-inflammatory effects. Yet a well-defined mechanism or cellular target for lidocaine or Zep peptides' anti-inflammatory effects has not been proposed.

We aimed to determine whether lidocaine and Zep peptides reduce the release of the proinflammatory cytokine, IL-1β, from activated human …

Preparation And In Vitro Characterization Of An Integrated Graft System For Regenerative Engineering, Mohammed Barajaa

Master's Theses

No abstract provided.

Language Abilities In Single- And Dual- Language Learners With Autism Or Other Developmental Disorders, Yael Dai

Master's Theses

Parents and providers worry that exposure to two languages will impair language acquisition in children with autism spectrum disorder (ASD) or other developmental delays (DD). However, limited research to date suggests that language milestones and abilities are unaffected. The current study explored language abilities in toddlers with ASD or DD exposed to one versus multiple languages, prior to any intervention. Results showed slower language acquisition in ASD than DD but no main effect of language exposure group or interaction of language group by diagnosis. This study suggests that bilingual parents can communicate with their children in both languages without harm …

Factors Associated With Violence Against Women In Rural India, Jennifer Lardner

Master's Theses

No abstract provided.

Human Dimensions Of Timber Rattlesnake (Crotalus Horridus) Management In Connecticut, Lindsay Keener-Eck

Master's Theses

No abstract provided.

Factors Influencing Maternal Involvement In Early Childhood: Exploring The Roles Of Maternal Stress And Children’S Narrative Representations Of Mothers, Rahmet Akpolat

Master's Theses

For children from low-income families, parent school involvement can be a crucial protective factor that supports children’s cognitive and emotional development. The present study seeks to explore how maternal stress in early childhood is associated with mothers’ later school involvement and whether children’s internalized positive, disciplinary and negative maternal representations act as potential mediators of this relationship. Currently, little research has been done on the contextual factors influencing parent school involvement in early childhood and this study aims to fill this gap in the literature. The data included 169 children and mothers from low-income families. Mothers completed questionnaires designed to …

Analysis Of A Newly Digitized Long-Term Dataset Of Environmental Observations From Long Island Sound, Jacob Snyder

Master's Theses

Project Oceanology, a non-profit oceanographic educational organization has been collecting data including pH, oxygen, and temperature conditions as well as abundances of benthic invertebrates and near-shore fish catches since 1972 from Eastern Long Island Sound. These data had been stored solely on single-copy paper sheets and were therefore inaccessible to analysis. I digitized more than 100,000 abiotic measurements and 50,000 species abundance and size data collected over the past 45 years, and developed a web-based SQL database housed on the Long Island Sound Integrated Coastal Observing System (LISICOS) server. The database will ultimately become a searchable, downloadable, user-friendly web-based tool …

Effect Of Land Use Types On The Distribution Of Carnivore Species In Santa Clara County, Ca., Logan Thompson

Master's Theses

Anthropogenic pressures such as habitat loss and hunting pressures have caused terrestrial mammalian carnivores to decline by 95–99% in many regions of the world. These species are especially susceptible to fragmented habitats and a diminishing food supply because of their slow reproductive rates and high metabolic demands. In biodiversity hotspots such as California, understanding the range of human impacts to species is critical. This project assessed carnivore species distribution and occupancy by placing remote camera traps in a protected Reserve, agricultural, and developed land use types. Mountain lions were only detected in the protected Reserve, and because of this coyotes …

Community Structure And Zonation Of Antarctic Benthic Invertebrates: Using A Remotely Operated Vehicle Under Ice To Define Biological Patterns, Dorota Szuta

Master's Theses

The Ross Sea, Antarctica is a deep bay of the Southern Ocean that exhibits seasonal sea ice and is adjacent to a permanent ice shelf overlying seawater. In 2008 and 2009, imagery of the seafloor under the McMurdo Ice Shelf and under the seasonal ice in the Ross Sea was collected via remotely operated vehicle (ROV) at depths to 300 m. Distinct differences in Antarctic benthic communities were observed over multiple environmental gradients. Species abundance typically exhibited a unimodal distribution with depth with mid-depth peaks, reflecting a food limitation at the deep end and potentially ice disturbance on the shallow …

Youth Sport Development Pathways And Experiences Of Ncaa Division I Women's College Soccer Players, Emily Hardell

Master's Theses

As youth sport has become increasingly professionalized, many believe that the route to elite level play is through early specialization. Early specialization is a contentious issue, and many risk factors have been associated with high levels and intensities of training in youth. Youth today participate in highly competitive sport in pursuit of elite levels of play, recognition, and financial gain. Early specialization is thought to be a requirement for advancement, yet little is known about the early experiences of team sport athletes who grew up in the US. This is the story of 15 elite female athletes who “made it” …

Eductional Persistence In Latino Male Students Attending University: Their Experiences And Identity Development, Michelle Swartz

Master's Theses

Because Latino male students have lower graduation rates from high school and postsecondary institutions than other demographic groups have, this qualitative research study examined the psychosocial experiences that influence the development of educational persistence. To this end, eight Latino male undergraduate students, who are also low-income and first-generation students at San José State University, participated in semi-structured interviews in which they discussed their identities and school experiences. An analysis of the eight interviews shows how these students made meaning of their education, gender identity, and ethnicity. The constant comparative method for analyzing interview transcripts revealed the following themes: (a) the …

Design And Analysis Of Bio-Inspired Nacelle For Current Energy Turbine, Michael Coe

Master's Theses

he world’s oceans contain a substantial amount of energy, stored in ocean currents and waves. Ocean current energy can be extracted using similar principles to existing wind energy platforms. A problem with such extraction methods is that the velocity of the fluid in ocean currents is too low for efficient energy generation. A potential solution to this problem is the use of a concentrator or diffuser to increase fluid velocity to the blades, but this carries with it extra costs. A more cost effective solution to this problem is presented by the use of a down flow current turbine in …

A Novel Model Of “Remedy And Elimination Of Tuberculosis”, Akanksha Mishra

Master's Theses

Tuberculosis (TB), is one of the top ten causes of death worldwide. TB is commonly linked to poverty and creates poverty as it is the single largest cause of death in the 15-49-year-old age group in South-East Asia (WHO, 2017). It is also linked to the HIV population, and its leading cause of death. TB is effected by socio-economic factors, cultural factors, and health policy. My goal of this dissertation is to identify systematic approaches that shall support the mission set by World Health Organization (WHO): to eliminate TB globally by the year 2050. Measures might include addressing social barriers …

Traditional Ecological Knowledge In Collaborative Forest Restoration In The Sierra Nevada, Michael Boero

Master's Theses

Forest restoration in the Sierra Nevada is receiving increased attention due to climate change, increasing populations in the wildland-urban interface, and the historical mismanagement of some forests, evidenced in part by the increasing frequency and severity of wildfires in recent years. In response to an urgency to implement restoration in the absence of consensus on the best ways to proceed, public land managers practice collaborative management approaches to ameliorate antagonism between stakeholders and allow restoration projects to proceed when agreement is difficult to achieve. Stakeholders often include representatives from local community groups, environmental organizations, private industry, and—notably—tribal groups, who hold …

A Full Factorial Analysis Of Polymerization Conditions Of Sodium Alginate Beads To Determine The Effect On Diffusivity And Concentration Limit Of Tryptophan And Glucose, Allison Schulkins

Master's Theses

The purpose of this work is to develop an experimental setup with associated mathematical analysis to analyze the changes in diffusivity through calcium alginate gel beads made under different polymerization conditions. The diffusivity of the solutes glucose and tryptophan was calculated by measuring the concentration change over time as solute diffused out of the alginate spheres and into the bulk. The alginate beads were generated using different polymerization conditions following a two-level, three-factor, full-factorial matrix with alginate concentration, cross-linker concentration, and cross-linking time as the factors. The diffusivities showed variations between different conditions, which were analyzed to determine the main …

Developmental Effects Of Nicotine Exposure In Drosophila Melanogaster, Lisa Janet Wong

Master's Theses

Approximately 12%-20% of pregnant women smoke at some point during pregnancy, and 10% of pregnant women are reported to have smoked during the last 3 months of pregnancy. Smoking during pregnancy leads to developmental health risks for the fetus and child, including increased mortality, low birth weight, and developmental delays. The direct molecular targets of nicotine are nicotinic acetylcholine receptors (nAChRs) due to the similarities in structure between nicotine and acetylcholine. However, in many cases, it remains unclear what molecular events downstream of nAChRs lead to the deleterious effects of nicotine on development. We have established Drosophila melanogaster as a …

Preparation And Characterization Of A (Viologen) Containing Main Chain Peg-Carbon Nanotubes Phase For Hybrid Supercapacitor Application, Kanishka Rana

Master's Theses

Supercapacitors (SCs) and batteries are recognized as the two most important electrical energy storage devices. SCs have some distinct advantages such as high-power density and long cycle life when compared to batteries. However, they have yet to match the energy densities of batteries. In an effort to improve the energy density of SCs, a hybrid supercapacitor was developed using a pair of single-walled carbon nanotube electrodes and two polyethylene glycol (PEG-400) gel-polymer electrolytes (GPEs) containing redox additives of viologen (V) and ferrocene dicarboxylic acid (Fc), respectively. The introduction of V and Fc, was expected to improve the energy performance of …