Digital Commons Network^™

Effects Of Omega-3 Fatty Acids And Hypoxia On Progesterone Biosynthesis And Mitochondrial Morphology In The Corpus Luteum, Brian Krum

Master's Theses

The corpus luteum is a transient steroidogenic endocrine gland that forms from the remnants of the ovulatory follicle. This gland is responsible for the synthesis and secretion of the hormone progesterone, which is critical for the establishment and maintenance of pregnancy. In a non-pregnant cow, PGF2a is released from the uterus to regress the corpus luteum. However, in the case of pregnancy, the embryo must mitigate PGF2a synthesis to prevent corpus luteum regression. If the embryonic signal is either too late or too weak, uterine PGF2a secretion may result in corpus luteum regression and termination of pregnancy. Omega-3 fatty acids …

Willingness To Seek Professional Help For Suicidal Thoughts: The Role Of Masculine Norms And Self-Stigma In Service Members, Samantha E. Daruwala

Master's Theses

Given the high rate of suicide, it is important to understand what barriers are preventing service members from seeking professional help for suicidal thoughts. Self-stigma and conformity to masculine norms, which are evident in the military culture, have been demonstrated as barriers to care in civilians. This study sought to better understand how these two factors are related to willingness to seek professional help for suicidal thoughts and a personal-emotional problem among service members. Specifically, we (1) identified service members’ willingness to seek help from a variety of sources for suicidal thoughts and a personal-emotional problem, (2) examined how conformity …

The Effects Of Optimism On Anti-Natalism, Faith L. Brown

Master's Theses

People sometimes hold opinions on others’ choices, particularly their reproductive decisions, as these choices are important decisions that impact the lives of multiple people. People can believe that everyone should have children (pro-natalism) or that everyone should refrain from having children (anti-natalism) or they can hold no position on the reproduction of others. The main justification for anti-natalism is that life contains more suffering than pleasure and that it would be better if new people were not born to experience this suffering. This is why some theorists argue that people reject anti-natalism irrespective of how bad …

Sustainable Wastewater Treatment In The American South, Caroline Miles

Master's Theses

Aging infrastructure and changing social and environmental conditions are two factors that are currently pushing wastewater treatment towards more sustainable methods. This thesis investigates what sustainable treatment technologies are being used in the American South through a focused sample of six cities across the region: Austin, Texas; Fayetteville, Arkansas; Hattiesburg, Mississippi; Memphis, Tennessee; Ocean Springs, Mississippi; and Tuscaloosa, Alabama. The study assesses the types of sustainable treatment technology utilized at each facility, the perspectives of wastewater professionals about the technology, and the economic and environmental sustainability of each facility. Data was collected through direct observations on tours of the facilities, …

Thiol-Ene Click Chemistry For Solid State Triplet-Triplet Annihilation Upconversion And Parts-Per-Billion Pyrophosphate Sensing In Artificial Seawater, Abagail K. Williams

Master's Theses

Thiol-ene click chemistry is a robust approach to molecularly engineering polymers for many applications. Within this work, thiol-ene click chemistry is used to fabricate thiol-ene networks for TTA-UC and to synthesize a conjugated polyelectrolyte (CPE) used as a pyrophosphate (PPi) sensor in complex aqueous media. Chapter I focuses on the synthesis and upconversion performance of rubbery networks fabricated using thiol-ene click photopolymerization. The advancement of triplet-triplet annihilation based upconversion (TTA-UC) in emerging technologies necessitates the development of solid-state systems that are readily accessible and broadly applicable. We demonstrate that thiol-ene click chemistry can be used as a facile cure-on-demand synthetic …

An Assessment Of The Use Of Photogrammetry In Cranial Metric And Non-Metric Studies, Amy Hair

Master's Theses

Methods in biological anthropology have made tremendous leaps in recent years and with the increasing rise in technology there is no reason to suspect that this trend will be decreasing. Particularly methods in 3D digitization have not only increased but have also become more accessible in bioarchaeology. One method, photogrammetry, offers bioarcheologists a unique opportunity to easily collect and process cranial metric and non-metric data that can be used to quantify biological relatedness. While these advances are expected to continue, it is ignorant to assume that they represent a fail proof solution. A critical examination is necessary to quantify the …

Sedimentary Dynamics And Water Quality In Floodplains Of The Lower Mississippi River Near Natchez, Mississippi, During The 2018 And 2019 Floods, Jansen Costello

Master's Theses

The Lower Mississippi River (LMR) near Natchez, Mississippi, experienced intervals of major flooding during the 2018 and 2019 water years. While federal agencies sample sediment and water quality in the main LMR channel, little is known about sedimentary dynamics, water quality, and nutrient concentrations of the overbank water column within the embanked LMR floodplain. These data are needed to support ecological floodplain restoration efforts and provide context for downstream concerns including sediment delivery to coastal zones and hypoxia in the Gulf of Mexico.

This study monitored the overbank flood pulses and associated sediment deposition along the LMR embanked floodplain during …

Nearshore Sedimentology Of Eroding Microtidal Estuaries: Bon Secour Bay, Alabama And Perdido Bay, Florida, Jennifer Simpson

Master's Theses

Estuarine shorelines along the northern Gulf of Mexico are dynamic geologic settings that provide numerous ecological and economic benefits. By definition, estuaries are semi-enclosed bodies of water that receive sediment from two sources: (1) fluvial systems feeding into the estuary and (2) sediment transported by wave action and tidal currents from adjacent marine sources. Erosion of estuarine shorelines resulting from rising sea level, storm impact, and anthropogenic influence has been increasingly evident in the microtidal Gulf Coast over recent decades. This study collects quantitative and qualitative data to better understand sedimentary dynamics associated with contemporary estuarine shoreline erosion in Bon …

A Change In Fish Assemblages In The Pascagoula River, Ms, Sara Barrett

Master's Theses

The Pascagoula River in southeastern Mississippi is the largest remaining un-impounded river system in the contiguous United States and have seen a change in fish assemblages over time. Examination of long-term fish assemblage data show that the mainstem Pascagoula River and its two major tributaries (mainstem Leaf and Chickasawhay Rivers) have been dominated by five abundant taxa that comprise 83% of individuals sampled, with Cyprinella venusta in the highest abundance. Beginning in about 2005-2006, a change in the mainstem Pascagoula River (but not mainstem Leaf and Chickasawhay rivers) was seen to an alternate assemblage where five different taxa dominate (89% …

Homotopy Analysis Method For Nonlinear Ordinary Eigenvalue Problems, Subagya Perera

Master's Theses

In this thesis, we solve nonlinear differential equations by the homotopy analysis method (HAM), which is a semi-analytic method first introduced by Shijun Liao in 1992. The modified HAM can be viewed as a more generalized method that encloses many perturbation and non-perturbation methods. It is different from perturbation or other analytical methods in that it allows considerable freedomformanyvariables. Using the modified HAM, especially zero-order and higher-order deformation equations, we solve a nonlinear initial value problem and a nonlinear eigenvalue problem. We adjust the convergence region of a solution by modifying auxiliary parameter values. The results converge in very few …

Mosquito Communities And Insect-Specific Viruses In A Heterogenous, Urban Landscape In Puerto Rico, Nicole Scavo

Master's Theses

The goal of my thesis was to investigate the relationships between mosquito diversity, Aedes aegptyi populations, and socioeconomic factors (SEF) in an urban setting and to characterize the virome of Ae. aegypti in San Juan, Puerto Rico. Mosquitoes were collected in three periods during late 2018 and early 2019 and were identified to the species level. Socioeconomic factors were measured using foot surveys and U.S. Census data. Some Ae. aegypti females were processed for sequencing and sent to Quick Biology for RNA sequencing to identify viruses. Results showed a link between SEF and Ae. aegypti abundance as well as between …

Elucidating The Molecular Pathways Involved In The Fitness And Water Stress Response Of Pseudomonas Synxantha 2-79 In The Plant Rhizosphere, Clint Henry D. Pablo

Master's Theses

Pseudomonas synxantha 2-79 is a biocontrol agent that represents beneficial indigenous rhizobacteria that are broadly distributed in the Pacific Northwest, USA and flourish in the rhizosphere of commercially grown wheat under surprisingly arid conditions. The molecular adaptation of 2-79-like bacteria to plants growing in dry soils is poorly understood. We hypothesized that the ability of 2-79 to colonize and persist in the rhizosphere of water-stressed plants is underpinned by the formation of hydrating biofilms and the utilization of root exudates that contain plant-derived osmoprotectants called quaternary ammonium compounds (QACs). We tested this hypothesis by identifying waters stress response pathways in …

Characterizing Erad And Antioxidant Response In Ixodes Scapularis Ise6 Cells During Borrelia Infection, Latoyia Downs

Master's Theses

Ticks are hematophagous ectoparasites that transmit a multitude of diseases to humans. Borrelia burgdorferi (BB) and Borrelia miyamotoi (BM) are both tick-borne pathogens that cause disease in humans and are transmitted by the black-legged tick (Ixodes scapularis). A byproduct of blood digestion generates reactive oxygen species that are toxic and cause oxidative stress which promotes cellular damage and dysfunction. The endoplasmic reticulum (ER) is especially affected by oxidative stress, resulting in a buildup of improperly folded proteins in the ER lumen called ER stress. To prevent cellular damage, the tick utilizes an antioxidant system to neutralize ROS and mechanisms …

Historical And Contemporary Variables Affecting The Range And Distribution Of Aedes Aegypti, The Yellow Fever Mosquito, In The United States, Nicole Mackey

Master's Theses

Aedes aegypti, the primary mosquito vector of the yellow fever virus, threatens global health by passing on this virus, as well as chikungunya, dengue, and Zika viruses. Through its natural tendency to live in highly urban areas and bite human hosts; understanding the factors that affect the historical and current range of the pest is invaluable (Gubler, 1998). Although these viruses are not normally found in the United States, lack of vaccinations and wide-spread presence of the mosquito could lead to these diseases being reintroduced with potentially devastating effects (Monath, 2001). To determine a partial historical range of A. aegypti …

Malaria Risk On Ancient Roman Roads: A Study And Application To Assessing Travel Decisions In Asia Minor By The Apostle Paul, Daniel C. Browning Jr

Master's Theses

This study models malaria risks for travelers on ancient Roman roads with the goal of providing a tool for historical assessment of travel accounts from antiquity. The project includes: identification of malaria risk factors and associated spatial datasets, malaria risk model construction, verification and validation against available pre-eradication data, overlay of ancient Roman road data, and an initial case-study application to the journeys of the Apostle Paul, as narrated in the New Testament book, Acts of the Apostles (Acts). The project is intentionally cross-disciplinary in bringing the technical capabilities of GIS to the task of evaluating nuanced textual sources for …

Identification Of Saliva Using Dna Methylation Analysis For Forensic Use, Elizabeth Staples

Master's Theses

The identification of biological fluids is a precursor to determine if further human identification is required in a forensic setting. There are four forensically-relevant biological fluids: blood, semen, vaginal epithelial tissue, and saliva. While serological testing can identify these tissue types to some degree of accuracy, there has recently been momentum in research to use DNA methylation for tissue identification.

In the current study, five potential tissue-specific methylation markers were studied in order to identify locations in the genome that would differentiate saliva from other tissue types. Genomic DNA was extracted from each sample, followed by bisulfite modification, polymerase chain …

Dna Methylation Analysis For Tissue And Age Determination For Forensic Human Identification, Lauren Satcher

Master's Theses

Forensic Science is a vital aspect of criminal investigations and new techniques that could be used to advance this field are constantly being investigated. There are current forensic techniques to identify relevant tissues, but tissue specific methylation markers are being examined as a new way for identifying tissue type. In addition, methylation markers are being investigated as a method to determine other relevant information about donors, such as the age of the individual.

In this study, methylation markers were investigated to determine tissue specific markers to differentiate sperm from blood, saliva, and vaginal epithelial cells, and age specific markers to …

A Time Integration Method Of Approximate Particular Solutions For Nonlinear Ordinary Differential Equations, Cyril Ocloo

Master's Theses

We consider a time-dependent method which is coupled with the method of approximate particular solutions (MAPS) of Delta-shaped basis functions and the method of fundamental solutions (MFS) to solve nonlinear ordinary differential equations. Firstly, we convert a nonlinear problem into a sequence of time-dependent non-homogeneous boundary value problems through a fictitious time integration method. The superposition principle is applied to split the numerical solution at each time step into an approximate particular solution and a homogeneous solution. Delta-shaped basis functions are used to provide an approximation of the source function at each time step. The purpose of this is to …

An Analysis Of Dredge Efficiency For Surfclam And Ocean Quahog Commercial Dredges, Leanne Poussard

Master's Theses

Between 1997 and 2011, The National Marine Fisheries Service conducted 50 depletion experiments to estimate survey gear efficiency and stock density for Atlantic surfclam (Spisula solidissima) and ocean quahog (Arctica islandica) populations using commercial hydraulic dredges. The Patch Model was formulated to estimate gear efficiency and organism density from the data. The range of efficiencies estimated is substantial, leading to uncertainty in the application of these estimates in stock assessment. Analysis of depletion experiment simulations showed that uncertainty in the estimates of gear efficiency from depletion experiments was reduced by higher numbers of dredge tows per experiment, more tow overlap …

Zebrafish Eye Development: Rac And The Creepy Crawlers Of The Eye, Zvi Murry

Master's Theses

During vertebrate eye development the optic vesicles protrude from either side of the brain and form the optic cups. As an optic cup starts to surround the lens a groove on the ventral side of the eye forms, known as the choroid fissure (CF). Normally, the CF will close around the optic nerve and hyaloid vasculature. If this process does not occur properly it results in a keyhole opening in the eye known as coloboma. This results in blindness and affects nearly 1 in 4-5,000 births. Zebrafish were utilized as a model for eye development to study CF closure (CFC) …

Type 3 T Helper Cell And Myeloid Derived Suppressor Cell Population Dynamics In A Mammary Carcinoma Model, Viva Rase

Master's Theses

Immunotherapies that augment Type I immunity show robust responses in diffuse blood cancers yet remain relatively ineffective in breast and other solid tumor malignancies. Breast tumor resistance to immunotherapies is associated with polarization towards pro-tumor Type 2 immunity, as well as the expansion of a myeloid derived suppressor cell (MDSC) population that inhibits Type 1 T helper (Th) and CD8+ cytotoxic T cells. Does polarization toward Type 3 immunity play a role in mammary tumor formation? This question had not been investigated prior to these studies despite established relationships between MDSCs and Type 3 Th cells in other inflammatory pathologies. …

The Role Of Gibberellic Acid In Aphid-Plant-Arbuscular Mycorrhizal Fungus Interactions, Drew Olson

Master's Theses

Arbuscular mycorrhizal (AM) fungi form beneficial associations with the roots of most terrestrial plants, which is mostly characterized by increased plant nutrient acquisition (phosphate and nitrogen) by the fungi in exchange for carbon and lipids from the plants. These associations can also enhance plant coping mechanisms to abiotic and biotic stresses. However, insect herbivores, such as aphids, can benefit from AM fungi-plant symbioses by altering plant defenses that are driven mainly by the phytohormones jasmonic acid (JA) and salicylic acid (SA). Nevertheless, other phytohormones can modulate plant defenses by altering the balance of the JA and SA signaling pathways, such …

The Synthesis And Characterization Of Gold Nanoparticles Prepared With Novel Thioether-Ionic Liquids, Joie Games

Master's Theses

Gold nanoparticles have found a broad range of applications in the biomedical field including in drug delivery and biosensor development.The latter applications take advantage of a unique optical property called surface plasmon resonance. Easily accessible “bottom-up” synthesis methods are commonly used for the preparation of metal nanoparticles, in which a metal precursor is reduced in the presence of a stabilizing agent to prevent aggregation. Ionic liquids have been used in “bottom up” metal nanoparticle syntheses as stabilizing agents and as a means to functionalize metal nanoparticles’ surface. Due to the strength of gold-sulfur interactions, thiol-stabilized gold nanostructures are of great …

Pismo Clams (Tivela Stultorum) In Califorina: Population Status, Habitat Associations, Reproduction, And Growth, Alexandria R. Marquardt

Master's Theses

Marine shellfish play a vital role in intertidal ecosystems and coastal communities, but many of these fisheries are small-scale and lack the necessary monitoring to ensure long-term sustainability. Effective management often requires information on key demographic parameters, such as population status, reproduction and growth. Pismo clams (Tivela stultorum) are a culturally important and iconic species in California, which supported a thriving commercial and recreational fishery throughout much of the 1900’s. However, Pismo clam populations have declined statewide in recent decades and are attributed to human harvest and predation by California sea otters (Enhydra lutris); However, no …

Evaluating Methods To Reduce Humpback Whale (Megaptera Novaeamgliae) Ship Strike Mortality In Nearshore Feeding Habitats Within The San Francisco Bay, Alisa Keenan

Master's Theses

Across our world’s oceans, whales are perpetually stuck and killed by large ships. Sea-based commercial trade is increasing on a global scale; therefore, more large ships are circumnavigating through biologically important whale habitats. This is of grave concern to marine resource managers, especially in the United States where major shipping corridors overlap critical whale habitat. It is feared that in these areas with high whale density, ship strikes are reducing populations of whales to levels below what is sustainable for threatened and endangered species, thereby hindering the recovery of depleted whale populations. Humpback whales, a species that is federally-listed as …

Educational Equity And Informal Stem Field Trip Programming, Sal Alper

Master's Theses

Informal STEM field trip programming is a large, yet under-researched area of the education landscape. Informal STEM education providers are often serving a more privileged section of society, leading to a risk of perpetuating inequalities seen throughout the education landscape. In an attempt to address the lack of research, this thesis explores the relationship between educational equity and informal STEM field trips. The intention was to collect data using a critical ethnography approach to the methods of qualitative questionnaire and interviews of informal STEM educators. A change in circumstances from the global pandemic of COVID-19 caused a shift in this …

Neural Representation Of Articulable And Inarticulable Novel Sound Contrasts: The Role Of The Dorsal Stream, David Saltzman

Master's Theses

No abstract provided.

Thermal Ecology Of The Federally Endangered Blunt-Nosed Leopard Lizard, Kathleen N. Ivey

Master's Theses

Recognizing how climate change will impact populations can aid in making decisions about approaches for conservation of endangered species. The Blunt-nosed Leopard Lizard (Gambelia sila) is a federally endangered species that, despite protection, remains in extremely arid, hot areas and may be at risk of extirpation due to climate change. We collected data on the field-active body temperatures, preferred body temperatures, and upper thermal tolerance of G. sila. We then described available thermal habitat using biophysical models, which allowed us to (1) describe patterns in lizard body temperatures, microhabitat temperatures, and lizard microhabitat use, (2) quantify the …

Valorization Of Carrot Processing Waste, Alexandra M. Duval

Master's Theses

Commercial carrot processors produce up to 175,000 tons of carrot waste annually. Carrot Mash (CM) is the term referring to the waste by-product of peeled baby carrot processing. Transportation of carrot processing waste is expensive due to its high- water content (approx. 83-95%). High in bioactive compounds (carotenoids) and dietary fibers, it is expected that its conversion into a value-added by-product is of interest to the carrot processing industry. Hemicellulose-rich plant materials have proven to be a source of oligosaccharides, which are known for their beneficial prebiotic activity. The objectives of this research were to: 1) determine the effect of …

Subject-Specific Finite Element Models Of The Human Knee For Transtibial Amputees To Analyze Tibial Cartilage Pressure For Gait, Cycling, And Elliptical Training, Jonathon Stearns

Master's Theses

It is estimated that approximately 10-12% of the adult population suffers from osteoarthritis (OA), with long reaching burdens personally and socioeconomically. OA also causes mild discomfort to severe pain in those suffering from the disease. The incidence rate of OA for individuals with transtibial amputations is much than average in the tibiofemoral joint (TF). It is well understood that abnormal articular cartilage stress, whether that be magnitude or location, increases the risk of developing OA. Finite element (FE) simulations can predict stress in the TF joint, many studies throughout the years have validated the technology used for this purpose. This …