Digital Commons Network^™

Optimization Of A Gfp Biosensor For The Discovery Of Novel Antibiotics, Drake Heinz

Master's Theses

In today’s world, there is an urgent need to discover novel antibiotics due to the rise of antibiotic resistance and the lack of new ones being approved. Building off of Hanson et al. and their reduction-oxidation sensitive green fluorescent protein (roGFP)(1), that we yeast codon optimized (2); I wanted to determine if I could create a real-time biosensor that has the ability to respond to ROS produced in the stress response triggered by antibiotics as a platform to identify novel antibiotics. To achieve this, I need to know if it will oligomerize in bacterial cytoplasm due to its two cysteines …

Effects Of Exercise On Myogenic Regulatory Factors In Cancer Cachexia-Induced Skeletal Muscle Wasting, Kelsey Daehlin

Master's Theses

Cancer cachexia is a complex multiorgan syndrome that affects bodily systems such as liver, brain, fat tissue, and skeletal muscle. It is a major factor that decreases quality of life in cancer patients and is related to about 20% of cancer deaths, however exercise has shown to be effective in helping combat the muscle wasting effects that cancer cachexia has on the body. Cachexia characterized by skeletal muscle wasting which may be caused by downregulation of positive myogenic regulatory factors such as MyoD and myogenin, and upregulation of negative myogenic regulatory factors such as myostatin and MuRF-1. While it is …

Quality Of Life Among Colorectal Cancer Patients During Chemotherapy, Chau Minh Than

Master's Theses

This study had the general goal of surveying the quality of life (QoL) and related factors affecting the QoL of colorectal cancer (CRC) patients participating in cancer chemotherapy at University Medical Center (UMC) in Ho Chi Minh City. The study also had the following specific objectives: (a) determining the demographic and clinical characteristics of CRC patients and (b) determining health-related quality of life (HRQoL) and related factors affecting HRQoL of CRC patients receiving cancer chemotherapy. A descriptive cross-sectional design was used. Sixty CRC patients being treated at the UMC participated in this study. The research used the European Organization for …

The Role Of Gibberellic Acid In Aphid-Plant-Arbuscular Mycorrhizal Fungus Interactions, Drew Olson

Master's Theses

Arbuscular mycorrhizal (AM) fungi form beneficial associations with the roots of most terrestrial plants, which is mostly characterized by increased plant nutrient acquisition (phosphate and nitrogen) by the fungi in exchange for carbon and lipids from the plants. These associations can also enhance plant coping mechanisms to abiotic and biotic stresses. However, insect herbivores, such as aphids, can benefit from AM fungi-plant symbioses by altering plant defenses that are driven mainly by the phytohormones jasmonic acid (JA) and salicylic acid (SA). Nevertheless, other phytohormones can modulate plant defenses by altering the balance of the JA and SA signaling pathways, such …

Pismo Clams (Tivela Stultorum) In Califorina: Population Status, Habitat Associations, Reproduction, And Growth, Alexandria R. Marquardt

Master's Theses

Marine shellfish play a vital role in intertidal ecosystems and coastal communities, but many of these fisheries are small-scale and lack the necessary monitoring to ensure long-term sustainability. Effective management often requires information on key demographic parameters, such as population status, reproduction and growth. Pismo clams (Tivela stultorum) are a culturally important and iconic species in California, which supported a thriving commercial and recreational fishery throughout much of the 1900’s. However, Pismo clam populations have declined statewide in recent decades and are attributed to human harvest and predation by California sea otters (Enhydra lutris); However, no …

Barriers To Health: Understanding The Barriers Faced By Community Intervention Projects, Vera Landrum

Master's Theses

Health disparities affect significant portions of the population and are most often experienced by marginalized communities (Wilkin, 2013). Health disparities are also impacted by geographical location, and hunger often affects rural areas (Dutta, Anaele, & Jones, 2013; Rural Health Information Hub, 2017). This study aims to understand how nonprofit organizations focused on addressing food insecurity disseminate information to members of the local community and how the input of community members impacted the overall food insecurity campaign. This study was conducted by interviewing five nonprofit directors and conducting three focus groups with clients of the. aforementioned organizations. The author found that …

Valorization Of Carrot Processing Waste, Alexandra M. Duval

Master's Theses

Commercial carrot processors produce up to 175,000 tons of carrot waste annually. Carrot Mash (CM) is the term referring to the waste by-product of peeled baby carrot processing. Transportation of carrot processing waste is expensive due to its high- water content (approx. 83-95%). High in bioactive compounds (carotenoids) and dietary fibers, it is expected that its conversion into a value-added by-product is of interest to the carrot processing industry. Hemicellulose-rich plant materials have proven to be a source of oligosaccharides, which are known for their beneficial prebiotic activity. The objectives of this research were to: 1) determine the effect of …

Risk Factors For Poor Birth Outcomes In Moderately Malnourished Pregnant Women In Sierra Leone, Candice A. Rodriguez

Master's Theses

Background: Maternal malnutrition in developing countries is associated with adverse pregnancy and birth outcomes. Malnourished mothers are often faced with additional risk factors as a circumstance of poverty. Supplementary nutrition can improve the outcomes of both mother and infant. Identifying maternal nutritional and socioeconomic risk factors is critical for developing effective interventions.

Objective: A secondary analysis to evaluate maternal risk factors associated with poor birth outcomes including pregnancy loss, low birth weight, stunting, and preterm delivery among moderately malnourished pregnant women in Sierra Leone. Maternal risk factors in the analysis are age, education, parity, BMI, MUAC, gestational weight gain, and …

Methods To Increase Algae Biomass Productivity In Raceway Pond Monocultures, Ryan S. Anderson

Master's Theses

The economics of algae biofuels and bioproducts would be improved by increased biomass productivity. Two studies on this potential are described in this thesis – one on a locally isolated filamentous yellow-green alga and the other on a planktonic strain genetically improved via selective enrichment.

Polycultures have been viewed as productive, stable, and, in some cases, harvestable by natural bioflocculation. Local native strains might have higher productivity than culture collection strains because they are already adapted to local outdoor conditions. In this study, the filamentous yellow-green alga Tribonema minus was isolated from a local volunteer polyculture. Its productivity as a …

Use Of Body Composition Imaging To Calculate 3-D Inertial Parameters For Inverse Dynamic Analysis Of Youth Pitching Arm Kinetics, Dalton James Jennings

Master's Theses

The objectives of this study were to 1) calculate participant-specific segment inertial parameters using dual energy X-ray absorptiometry (DXA) data (referred to as full DXA-driven parameters) and compare the pitching arm kinetic predictions using full DXA-driven inverse dynamics vs scaled, DXA mass-driven (using DXA masses but scaled centers of mass and radii of gyration), and DXA scaled inverse dynamics(ID) (using the full DXA-driven inertial parameters averaged across all participants), 2) examine associations between full DXA-driven kinetics and body mass index (BMI) and 3) examine associations between full DXA-driven kinetics and segment mass index (SMI). Eighteen 10- to 11- year-olds pitched …

Inactivation Of Foodborne Pathogens During Cider Fermentation, In A Cider Model System And Commerical Cider, Kathryn K. Yamada

Master's Theses

Hard cider is an alcoholic drink made from fermented crushed fruit, typically apples. The popularity of this fermented alcoholic beverage has been on the rise within the last decade. Historically, hard cider has been deemed safe due to the presence of ethanol and the low pH. Although there is lack of scientific evidence to prove that hard cider will and can be safe from foodborne pathogens. Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes are three predominate foodborne bacterial pathogens of concern in the food and beverage industry. Escherichia coli O157:H7 in particular has been associated with fresh produce and …

The Effect Of Time-Restricted Feeding On Body Weight, Energy, Mood, Sleep, And Hunger Levels Of Adults On Social Media, Rachel Elizabeth Haines

Master's Theses

The purpose of this study was to investigate the effect of time-restricted eighthour feeding on body weight, self-reported energy, mood, and hunger scores, and selfreported sleep patterns of adult men and women on social media. Generally healthy adults ages 18 to 65 were invited on social media to participate in a five-week study which involved a one week of following their normal dietary patterns succeeded by four weeks of practicing time-restricted feeding of eight hours per day, also known as 16 hours of fasting. Daily surveys were created in Survey Monkey and distributed through the messaging platform, Remind, that gathered …

A Multi-State Evaluation Of The Knowledge Of The Background And Dangers Of Popular Fad Diets Among Cooperative Extension Agents In Family And Consumer Sciences, Abigail Paige Mcalister

Master's Theses

Cooperative extension provides communities with research-based information in several areas including family and consumer sciences (FCS) (Franz & Townson, 2008). Because FCS agents teach communities about dietary guidelines, it is important that they are aware of the latest nutrition research. This study assessed the knowledge of popular fad diets and their potential adverse effects among FCS cooperative extension agents, and its relationship among the variables of location, professional certifications held, years of extension work experience, membership in professional associations, and education level. FCS extension agents in Louisiana, Texas, Arkansas, and Mississippi were emailed an online survey, which collected demographics and …

Disordered Eating In Collegiate Athletes: Prevalence And Relationships With Body Composition, Sofia Apsey

Master's Theses

Performance pressures and emphasis on body weight and shape put collegiate athletes at risk for disordered eating. If not identified early, disordered eating behaviors could develop into a clinical eating disorder. While such behaviors are a known problem, prevalence rates are not well established in collegiate athletes. Disordered eating may also relate to physiological changes in athletes, such as body composition and performance, although this relationship is not well understood. The aims of this research were to 1) determine the prevalence of disordered eating risk in NCAA Division I student-athletes and 2) determine if such behaviors are related to baseline …

Expression And Purification Of Snake Antivenom Peptide In Pichia Pastoris, Israel Juarez Contreras

Master's Theses

It has been shown that a peptide of the first 10-15 N-terminal amino acids of the lethal toxin neutralizing factor (LTNF) protein found in opossums (Didelphis spp.) holds promise as a low-cost therapy for snake envenomation. To date, the 11-mer has been expressed in E. coli and shown to neutralize snake venoms. However, possible endotoxin concerns warrant an investigation into other microbial hosts. The methylotrophic yeast, Pichia pastoris, shows promise as an alternative host. Active LTNF peptide was expressed and purified in P. pastoris. This was accomplished by subcloning a tandem repeat of the first 15 N-terminal amino acids of …

Climate-Driven Insect Herbivory In Mixed Coast Live Oak Woodlands Within The Mt. Hamilton Range, Santa Clara County, Michelle Domocol

Master's Theses

Climate change is expected to dramatically alter and destabilize critical functions of oak woodlands (Quercus spp.) in California. Oak woodlands support hundreds of vertebrate species and thousands of native insect species. Climate-driven changes in annual temperature, annual rainfall, and spatial climatic variability may increase insect herbivore pressures on mixed coast live oak (Quercus agrifolia) woodlands. The Mt. Hamilton Range of Santa Clara County, California offers a unique matrix to study oak-insect herbivore relationship using elevation as a proxy for climate change. This thesis research assessed the relationship between lepidopteran herbivory and coast live oak with insect surveys and the laboratory …

Association Of Food Pharmacy Participation With Type Ii Diabetes Risk Factors, Breanne Vinogradoff

Master's Theses

Food insecurity is defined as a lack of access to enough food for a healthy lifestyle and has been found to lead to higher prevalence of type 2 diabetes (T2DM). In recognition of this relationship, many safety-net clinics and food banks have introduced “food pharmacies.” The term “food pharmacy” may be used to describe a treatment model that focuses on provision of disease-specific, medically appropriate food. This observational study examined data from a food pharmacy in the San Francisco Bay Area to determine if program participation improved clinical measures of T2DM status or risk, namely glycated hemoglobin (HbA1c), fasting blood …

The Effects Of A 6-Week Group Meal Prep Program On The Frequency Of Consumption Of Home-Cooked Meals, Shannon Mendez

Master's Theses

Consumption of food away from home is associated with higher caloric intake and poorer diet quality compared to home-cooked food. The advance quantity meal prep (AQMP) program is a group-based program developed to increase the frequency of consumption of home-cooked meals. The AQMP pilot study was a pre-experimental 6-week intervention aimed at analyzing the effects of the program on frequency of consumption of home-cooked meals, cooking attitudes, cooking self-efficacy, and anthropometric measures. Ten study participants met once a week at a commercial kitchen to prepare and package lunches, dinners, and snacks for the work week. A survey was administered and …

Comparing Levels Of Environmental Literacy Between Stars- And Non-Stars-Rated Universities, Analisa Kathleen Campos

Master's Theses

The Sustainability Tracking, Assessment, and Rating System (STARS) is a program that measures university sustainability levels. STARS ranks university campuses based on the number of points received from individual specifications such as green buildings, access to mass transit, number of environmental courses, and more. Therefore, the rank a school receives translates directly to its level of sustainability as an institution. Currently, it is unknown if there is a relationship between a campus’s STARS-rating and individual students’ environmental literacy (EL). The purpose of this project was to analyze the impacts of university’s sustainable features on student EL. Surveys were administered to …

Conceptualizing The Promise Of Life Skills Education For Adolescent Girls In The Global South: A Critical Discourse Analysis, Olivia Casey

Master's Theses

In recent years, international development organizations have increasingly championed life skills education as a crucial tool for empowering girls in the global South. However, while life skills education continues to gain momentum and popularity within the international development landscape, the heterogeneity of life skills interventions remains significant. In this study, I aimed to uncover how key global actors differently conceptualize life skills and the promises they are understood to hold for adolescent girls in the global South at the current moment. Utilizing the methodology of critical discourse analysis, I examined key documents on girls’ life skills education published by central …

Intramuscular Injection Of Plant Derived Antimicrobials: A Potenial Use Against M.Bovis In Cattle, Elizabeth Johnson

Master's Theses

Plant derived antimicrobials (PDAs) such as trans-cinnamaldehyde, eugenol and carvacrol, possess strong antibacterial properties yet are generally regarded as safe. In the current crisis of antibiotic resistance, the use of PDAs as alternatives to prevent and control bacterial infections in livestock has gained momentum. The inhibitory effects of PDAs on common bacteria such as C.difficile, Listeria, Salmonella have been widely studied. PDAs have also been found effective in wall-less bacteria such as Mycoplasma, for which common antibiotics are not effective and vaccines are not always effective. In animal husbandry, PDAs can be potentially added in feed to metaphalactively prevent …

Best Practices For Renovating Golf Course Fairways To More Sustainable Bentgrasses In The Northeastern United States, Zoe Esponda

Master's Theses

No abstract provided.

Nurses' Level Of Comfort, Familiarity, And Knowledge About Diabetes Management In Vinmec Central Park International Hospital, Nguyen Thi Xuan Mai

Master's Theses

Clinical nurses have a key role in supporting and management of diabetes patients during hospitalization. The purpose of this study was to evaluate nurses’ level of comfort, familiarity, and knowledge in diabetes management. In this study, 30 full-time nurses working in Vinmec Central Park International Hospital completed a 34-question assessment that included eight questions on comfort, six questions on familiarity, and 20 questions on nursing knowledge. The findings indicated the level of nursing knowledge regarding diabetes management in the hospital was low. The comfort, familiarity, and knowledge scores were not directly proportional to the age, education, and years of clinical …

Distribution, Abundance, And Trophic Ecology Of Anguilliform Leptocephali In The Northern Gulf Of Mexico, Lillian Collins

Master's Theses

All species of anguilliform eels have pelagic leptocephalus larval stages, which provides a means of monitoring the population dynamics of these otherwise cryptic and hard to study species. The goals of this study were to: 1) describe the distribution and abundance of anguilliform eel leptocephali across the northern Gulf of Mexico, and 2) examine the trophic ecology of leptocephali using stable isotope values of carbon and nitrogen (δ13C and δ15N). A 25-year (1990-2014) ichthyoplankton dataset provided by the National Oceanic and Atmospheric Administration (NOAA) Southeast Area Monitoring and Assessment Program (SEAMAP) was used to examine interannual variations in leptocephalus distribution, …

Archaeobotanical Analyses Of The Winterville Mounds Site (22ws500) And Other Southeastern Ceremonial Complexes, Dana Hauffe

Master's Theses

The prehistoric Southeast region of the United States has had very limited archaeobotanical research focused on botanicals’ medicinal or ritualistic characteristics. An analysis of reported botanical remains recovered from Winterville Mounds (22WS500) and seventy- two other Late Woodland and Mississippian sites, from seven states, was conducted to identify their potential medicinal and ritual use of seventy-five botanicals based on reported ethnobotanical evidence. By classifying botanicals into four classes, modeled after the dissertation of Dr. Michele Williams in 2000, taxon frequency and feature ubiquity is configured and utilized to identify the possible ceremonial and medicinal use plants. At Winterville Mounds Site …

Estimating Body Mass Through Bone Mineral Density Studies Using Dexa (Dual-Energy X-Ray Absorptiometry), Kaitlin Harstine

Master's Theses

Identification of a decedent is one of the primary goals of forensic anthropologists. In order to do this, one must build a biological profile based on the remains that are provided. Sex, age, ancestry, and stature are four of the most common, however a fifth addition piece of information that could be beneficial is body mass. The goal of this research is to explore the relationship between bone mineral density (BMD) and body mass, using data collected from dual x-ray absorptiometry (DEXA) scans of 107 collegiate football athletes. Athletes are a good fit for this study because they have achieved …

The Power Of Dance: How Dance Effects Mental And Emotional Health And Self-Confidence In Young Adults, Amber Salo

Master's Theses

The purpose of this study was to investigate the mental and emotional benefits of dance in young adults. The researcher directed and produced a free, one-day dance workshop that served as the format for the research event. The study involved sixty-three dance students, ranging in age from fourteen through twenty-five, including all years of high school, college, and post-graduation. The classes offered as part of this study were geared toward the joys of dance, setting and attaining goals, having an open, growth mindset, and being self-confident. The study incorporated both qualitative and quantitative research as a means to answer three …

Patient Knowledge About Disease Self-Management Of Cirrhosis, Thuy Xuan Trinh

Master's Theses

This study aimed to define the level of understanding of self-management for patients with liver cirrhosis. A descriptive cross-sectional study was conducted. Thirty cirrhotic patients (14 females and 16 males) in the Gastroenterology Department of University Medical Center, Ho Chi Minh City in Vietnam participated in answering the questionnaire to evaluate their knowledge about liver disease self-management. The majority of cirrhotic patients had an unsatisfactory level of knowledge regarding self management of their disease, particularly in terms of recognizing and preventing complications from hepatic encephalopathy, monitoring for liver cancer, and awareness of medications they should not use. Implications of the …

The Relationship Between Nutritional Status And The Quality Of Life For Gastric Cancer Patients, Van Thi Hong Nguyen

Master's Theses

Cancer patients often suffer from malnutrition and low quality of life, especially gastric cancer patients. This is an important factor in the decision to treat cancer. Researchers are interested in the relationship of nutritional status between quality of life through body mass index to the main aspects of quality of life: core healthy days, physical, mental, or emotional problems; physical, functional, cognitive, and social limitations; and side effects such as fatigue; nausea, pain, anorexia, constipation, and diarrhea. The relationship between quality of life and weight loss indicates the importance of nutrition management in cancer patients. Cancer patients have special physical …

The Thiol Specific Antioxidant (Tsa1) Gene Is Required For Survival In Macrophages And Oxidative Stress Resistance In Histoplasma Capsulatum, Lauren Kennedy

Master's Theses

Histoplasma capsulatum(Hc) is a pathogenic fungus that causes one of the most common invasive fungal respiratory diseases, Histoplasmosis. Histoplasmaundergoes a dimorphic shift from mold to yeast which is crucial to pathogenesis of the organism.

The thiol specific antioxidant gene, Tsa1,is strongly upregulated in the yeast (pathogenic) morphotype. This data led to the hypothesis that this gene plays a role in protecting Hcfrom host mediated oxidative attack. To characterize Tsa1 function, a knockdown strain (tsa1-RNAi) was created by RNAi gene silencing. Expression of Tsa1in the tsa1-RNAistrain was reduced to 10% that …