Digital Commons Network^™

Hypoxic Incubation Chamber, Simone Lisette Helfrich, Makenzie Nicole Jones

Master's Theses

This paper describes the design, manufacturing, and testing of a novel controllable hypoxic incubator with fully functional oxygen gas control and temperature control in a humid environment. On the current market, a majority of the few hypoxic incubators use pre-mixed gas that does not offer precise control over gas concentration. The objective for this project was to create a chamber that allows the user to set the O2 concentration to varying set points of % O2 while maintaining the chamber at a constant body temperature, CO2 level, humidity, and sterility. To start the project, multiple concepts were developed for the …

How Chicago Public Schools Engaged African American Parents In The Closing Of Austin Career Academy High School, Shani Blackwell

Master's Theses

The role of African American parents in education reform initiatives in Chicago is not clearly defined. Local School Councils (LSC) allow parents to make school related decisions in Chicago. But LSCs are removed from decisions about whether or not to place school on or off probation, and whether or not to close schools based on performance or building utilization. In 2013, African American and Latinx parents expressed outrage over the decision by Chicago Public Schools (CPS) to close 49 schools with protests, including a hunger strike by a group of parents, and pleas to the Chicago Board of Education (BOE). …

Exploring The Role Of Lactobacillus Jensenii And Lactobacillus Mulieris In The Urogenital Tract, Adriana Daniela Ene

Master's Theses

Lactobacillus is a predominant species of the urogenital tract and it has been found in females with and without lower urinary tract symptoms. The Lactobacillus genus is thought to be beneficial to the host due to its capability to produce hydrogen peroxide, secondary metabolites, and lactic acid. L. jensenii is an organism frequently isolated from the urogenital tract. In March 2020, a new sister taxon of L. jensenii was described, L. mulieris. Shortly thereafter we produced a genomic analysis of all publicly available genomes (n = 43) which reclassified some L. jensenii as L. mulieris. This motivated the in-depth study …

Youth Culture, Organizational Values, And Participatory Politics In The Digital Spaces Of Youth-Led Anti-Gun Violence Movements, Julie M. Szamocki

Master's Theses

As traditional forms of participating in politics are waning in popularity and younger people are becoming more politically aware, new ways to engage in politics are emerging from the intersection of youth culture and digital spaces. One way of understanding this intersection of activism and youth culture is to analyze how youth-led activist organizations engage in participatory politics in the digital space. I analyze how anti-gun violence organizations March for Our Lives and GoodKids MadCity, led by youth activists, reflect a unique and distinct form of digital engagement and organizational culture. Through content analysis, I will be highlighting organizational comparison …

Connecting School Climate And Trust: A Content Analysis Of Elements Of Trust In Student And Staff School Climate Surveys, Katherine Thyen

Master's Theses

This study aims to make a concrete connection between trust in schools and school climate. Research indicates that both trust and school climate can positively impact school experiences and performance for teachers and students, but no formal connection has been made between these two elements of schools. In order to answer this study’s research questions of “What differences in frequency and context of trust dimensions exist between student surveys and teacher surveys?” and “How are survey items related to trust dispersed across school climate domains?”, a directed qualitative content analysis was performed. School climate surveys administered to students and teachers …

Cataloging And Engineering Temperate Coliphages Of The Human Urinary Microbiome, Elias Crum

Master's Theses

In the US, around 60% of females will be diagnosed with a Urinary Tract Infection (UTI) in their lifetime, and Escherichia coli is the most implicated etiological agent in UTIs. Despite its frequent association with lower urinary tract symptoms, recent studies have found that the urinary microbiome (UMB), the viral, bacterial, and fungal resident members of the urinary tract, of healthy females can also consist of E. coli. While most research has focused on the bacterial constituents of the UMB, bacteriophages, viruses that infect bacteria, are far more abundant. Bacteriophages (phages) of other human microbiomes have been shown to play …

Constructing Planetary Citizens Through Ecopedagogy In U.S. Social Studies Standards, Madeline Anne Rainey

Master's Theses

The world is in the age of the Anthropocene, where humans are impacting the environment to disastrous effects. The capitalist economy, promoting neoliberal policies of mass consumption, has exacerbated the world's environmental deterioration and social inequity. The rights and responsibilities people hold have been rapidly changing with the fourth industrial revolution. Globalization and Information and Communications Technology (ICT) have further expanded notions of citizenship. While there have been numerous attempts to bring the environment into schools, it has not emphasized what this crisis deserves. Ecopedagogy, as a critical theory, explicitly examines the interplay between environmental and social problems and challenges …

Interrogating Antagonistic Differences Among Pseudomonas Aeruginosa Cdia Alleles, Haotian Yang

Master's Theses

Bacteria are complex social organisms that live within mixed communities where microorganisms compete for limited resources. We studied Contact-Dependent Growth Inhibition (CDI), a particular type of competition mediated by a type 5b, or two-partner, secretion system that is widely spread among Gram-negative bacteria. CdiB and CdiA make up the two-partner secretion system that mediates CDI. CdiB translocates CdiA to the cell surface, and CdiA delivers its C-terminal toxin domain to the target cell. The C-terminal toxin (Tox) domain of CdiA is highly diverse. We studied Tox domains of various Pseudomonas aeruginosa clinical isolates. We found different strains that contain unique …

Role Of Tetraspanins In Sars-Cov-2 Fusion And Entry, Marcos Saul Santiago Figueroa

Master's Theses

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the pandemic the world has been facing in recent years. Even as new countermeasures are developed, there is still much we don’t know in terms of its entry into host cells. Tetraspanins are transmembrane proteins that are near ubiquitous amongst cell types. They fulfill numerous roles, including that of a viral co-receptor. Here, we considered whether tetraspanins, specifically CD9, CD63, and CD81 influence SARS-CoV-2 fusion and entry. Using ACE2-LgBit and tetraspanin overexpressing EVs and HeLa cells, we find that the presence of excess tetraspanins inhibit fusion and entry. However, we …

To Be Fair: Colorism And Online Dating Among Young South Asians, Kajal S. Patel

Master's Theses

Online dating is rooted in first impressions through images. Because of this, users of these apps tend to alter their profile pictures to fit a certain criteria that they believe will attract more matches. For people of color, this benchmark is based upon fair skin and European facial features. This has led me to ask, how is colorism reinforced in online dating apps for Indian Americans, and more specifically though, how are the users of these apps portraying themselves in their own profiles as it relates to colorism? Due to various factors Indians with darker skin tend to feel pressure …

Removing Soluble Phosphorus From Tertiary Municipal Wastewater Using Phosphorus- Deprived, Filamentous Microalgae, Aloysia Ahern

Master's Theses

Harmful algal blooms (HABs) can be detrimental to ecosystems, human health, and economies. The low levels of phosphorus remaining in the effluent of municipal wastewater treatment plants can contribute to HAB formation. To achieve more complete phosphorus removal, an effluent treatment method has been proposed that uses phosphorus-deprived, filamentous microalgae to quickly assimilate soluble phosphorus to low concentrations. This study investigated two parameters that influence the feasibility of such a system: (1) the biomass growth productivity of algal cultures during the phosphorus deprivation period and (2) the correlation between the duration of this period and the phosphorus uptake rate by …

An Integrated Approach For Controlling Verticillium Wilt Of Strawberry, Jack T. Koster

Master's Theses

Strawberry (Fragaria x ananassa, Duch.) is an important crop in California, with more than 35,000 acres planted in 2018 resulting in a farm gate value of $3.1 billion. In 2020, California strawberry production accounted for more than 85% of national strawberry production and faces serious threats to production due to various soil-borne diseases. One such disease, Verticillium wilt, is caused by the fungal pathogen Verticillium dahliae and is commonly found in temperate zones around the world where strawberries are grown. Due to the phase-out of efficacious fumigants like methyl bromide, alternative disease management methods have become necessary to …

Analysis Of Current And Future Flood Hazard In The Sacramento-San Joaquin Delta, Nicholas L. Mcguire

Master's Theses

The primary motivation of this study is to estimate the current and future flood hazard throughout the Sacramento-San Joaquin Delta in the context of sea-level rise. I also analyzed the effects of storm surge and river flow on extreme events to better understand how these events originate and vary spatially and in time. To address this goal, I combined digital water level records (primarily 1983-present) with archival data collected by the California Department of Water Resources (1929-1983) to reevaluate flood hazard in the Delta and investigate the possible sensitivity of the region different sea-level rise projections. Available archival records from …

Determination Of Fungicide Resistance In Botrytis Cinerea On Wine Grapes In California's Central Coast Region, Evelyn Alvarez-Mendoza

Master's Theses

Botrytis bunch rot, caused by Botrytis cinerea, is a fungal disease that primarily affects the fruit of wine grapes. Infection of fruit consequently results in reduced yields and wine quality. These factors lead to significant economic losses for growers which prompts the implementation of management practices to control the disease. One objective of this study was to evaluate the level of resistance that populations of B. cinerea in the Central Coast region showed to various chemicals. A fungicide assay was conducted to determine resistant phenotypes to six fungicide active ingredients (pyrimethanil, iprodione, fenhexamid, fludioxonil, trifloxystrobin, boscalid). Thirty-five (2020) and …

Mechanisms Of Substrate Selectivity And Transport By Escherichia Coli Atp- Binding Cassette (Abc) Transporter Metni, Yuchun Chen

Master's Theses

ABC transporters are central in many cellular functions including nutrient uptake, signal transduction membrane assembly, and cellular detoxification. Both structural and functional studies have revealed insights into the high-affinity uptake mechanism of the MetNI methionine ABC transporter. Using the energy from ATP binding and hydrolysis, the MetNI-Q system can import L-Met, D-Met, and other methionine derivatives against concentration gradients. Many mechanistic studies of ABC transporter propose a model in which cognate binding proteins sequester substrates in the periplasm and deliver them to the transporter. In contrast, recent in vivo and crystallographic studies of MetNI-Q suggest that some substrates may be …

Thermodynamic And Structural Quantification Of Metal Ions Binding To The Antimicrobial Peptide Calcitermin, Sohee Choi

Master's Theses

Over the past several decades the number of antibiotic resistant bacteria has grown dramatically, in part due to the over-prescription of antibiotics. This rise in antibiotic resistance has led to development of novel antibiotics. An attractive alternative to treat bacterial infections may be the use of antimicrobial peptides due their little-known resistance from bacteria. One such peptide is calcitermin (VAIALKAAHYHTHKE), a 15-residue peptide found in human airways. Calcitermin has antimicrobial properties that target Gram-positive bacteria and is most active in acidic buffer (pH 5.4) due to protonation of its three histidine residues, which allows it to interact with the negatively …

Synthesis And Analysis Of Short Antimicrobial Peptides: Rr, Rika Igr, And Hq, Kathleen Pierce

Master's Theses

A significant impending threat to the public health system is the rise of multidrug resistant bacteria. An attractive potential option to combat this is antimicrobial peptides (AMPs). AMPs are peptide chains with lengths that range from 10 to 70 amino acid residues that show antimicrobial activity. AMPs tend to have less toxicity than other antibiotic therapies, have a broader range of activity, and have a decreased resistance development by the target cells. Short AMPs are especially attractive due to their stability as well as their ability to penetrate cell membranes. With a wide range of possible AMPs available, further characterization …

Machine Learning And Software Solutions For Data Quality Assessment In Cern’S Atlas Experiment, Cary Randazzo

Master's Theses

The Large Hadron Collider (LHC) is home to multiple particle physics experiments designed to verify the standard model and push our understanding of the universe to its limits. The ATLAS detector is one of the large general-purpose experiments that make use of the LHC and generates a significant amount of data as part of its regular operations. Prior to physics analysis, this data is cleaned through a data assessment process which involves significant operator resources. With the evolution of the field of machine learning and anomaly detection, there is great opportunity to upgrade the ATLAS Data Quality Monitoring Framework to …

No Justice, No Peace: An Examination Of The Protestor Experience, Naya Russell-Coprich

Master's Theses

More than 95% of the protests that took place in the summer of 2020 were deemed peaceful. However, many of these protests ended with violent encounters with police officers (Major Cities Chiefs Association, 2020). The purpose of this research was to explore the experience of protestors in America. This study also sought to identify, if at all, the disparities present when deciding to use excessive force against protestors. The sample for this study included students from the University of Northern Colorado, who were 18 years of age or older, and have attended a protest in their lifetime. The final sample …

Digital Pastoral, Rebecca Holifield

Master's Theses

Digital Pastoral is a collection of original poems and a creative nonfiction essay, accompanied by a critical introduction.

Infidelity And The Bioecological Protective Factors For Mental Health: A Longitudinal Investigation, Lindy Williams

Master's Theses

Infidelity is an important topic of research due to the profound effects on the individual experiencing the trauma. Through the lens of bioecological systems and betrayal trauma, the impact of infidelity on mental health is examined in relation to the most proximal systems to an individual which include personality traits such as agreeableness and neuroticism as well as support from friends, family, and community. This research explores the hypotheses that more support leads to more positive mental health outcomes after the experience of infidelity and the possibility that support from friends, family, and community may buffer specific personality traits and …

Alt-Right Of The_Donald And Authoritarian Communists On Reddit: Internet Memes To Build Community, Joshua Hendricks

Master's Theses

This project infuses Lacanian psychoanalysis further into the rhetorical study by exploring authoritarian discourses on Reddit. I analyze the alt-right community that found a home on the subreddit r/The_Donald and chart their discourse as it shaped from the quarantine to the establishment of an independent forum called TheDonald.win. I selected memes and the comments around them to build the analysis. The second aspect of this thesis examines authoritarian communist discourse on Reddit. The leftist community splintered when r/socialism banned a community member because the person made catgirl art. The ban sent shockwaves through the leftist community on Reddit and generated …

Fabrication Of Lab-Scale Polymeric And Silicon Dioxide Nanoparticle-Enabled Thin Film Composite Reverse Osmosis Membranes For Potable Reuse Applications, Timothy J. Dinh

Master's Theses

Reverse osmosis (RO) is widely used for water reclamation and is one of the most feasible technologies for addressing water scarcity around the world. RO membrane fabrication procedures are continually being optimized and modified to enhance the treatment performance and efficacy of the RO process. A review of the existing literature published on membrane fabrication revealed that a detailed and reproducible methodology consistent among prior studies was not available. Therefore, the primary objective of this study was to utilize techniques from prior research to develop a reliable lab-scale membrane fabrication process for studying the potable reuse applications of TFC RO …

Validation In Vietnam: Motivations And Experiences Of Vietnam Veterans Who Returned To Vietnam As Tourists, Brian Washam Ii

Master's Theses

The current historiography on the memory of the Vietnam War has primarily looked at how the collective memory of the war has been constructed through various factors. Scholars such as Jerry Lembcke, Patrick Hagopian, and Marita Sturken tend to examine monuments, film, and oral histories to establish a basis for how the memory of the Vietnam War was constructed and how these legacies from the war shaped the U.S. as a society going forward. Recently, scholars have begun looking more at the return trips of veterans to Vietnam as a source for understanding how veterans remembered their service.

By engaging …

Relationship Among Department Satisfaction, Training, And Police Behavior, Matthew Garvin

Master's Theses

The call for police reform has been sparked by incidents involving the shooting and killing of individuals by the police. Public and political scrutiny of police behavior and procedures has created a social identity of “Us Versus Them” mentality among police officers. Proactive policing strategies have diminished in response to the heightened scrutiny of police procedures and behavior, in turn, initiating a reduction in police protection and an increase in crime. While research has identified the importance of police presence, the literature does not yet answer questions regarding factors which influence police proactivity. To address the gap in understanding, this …

Evolving Deforestation Narratives In Practice: A Study Of The Un-Redd+ Readiness Programme In Nigeria, Stuart Clive Blackwell

Master's Theses

The UN-REDD+ Readiness Programme was conducted between 2009 and 2015. The REDD+ Programme in Nigeria began once the readiness program was completed. This study conducts a document analysis of the program utilizing a political ecology lens to determine the extent to which the program adheres to or strays away from critical narratives surrounding both drivers of deforestation and inclusion and autonomy of federal, state, and local communities. By examining the entirety of the available documentation of the REDD+ Readiness program in Nigeria using a political ecological lens, this study demonstrates both an evolution of the program itself, and an evolution …

"Conserving" The Middle Ground: Tennessee's Unionist Press In The Secession Crisis, 1860-1861, Michael Singleton

Master's Theses

This thesis advances scholars’ understanding of how newspaper editors framed and presented news during the secession crisis of 1860-1861. Methodologically, it draws on the publications of seven Unionist editors from Tennessee who initially resisted secession but later pursued different courses during the Civil War. Through this period, editors balanced their roles as journalists and political actors working to advance an ideological cause. Guided by existing practices and their unique journalistic styles, these editors presented a near unified message—influenced by Whig political culture—that framed their response to outside events. This unanimity fractured in 1861 as local pressures, business interests, and personal …

Legislative Language For Success, Sanjana Gundala

Master's Theses

Legislative committee meetings are an integral part of the lawmaking process for local and state bills. The testimony presented during these meetings is a large factor in the outcome of the proposed bill. This research uses Natural Language Processing and Machine Learning techniques to analyze testimonies from California Legislative committee meetings from 2015-2016 in order to identify what aspects of a testimony makes it successful. A testimony is considered successful if the alignment of the testimony matches the bill outcome (alignment is "For" and the bill passes or alignment is "Against" and the bill fails). The process of finding what …

Impact Of Incorporating Intuitive Eating Principles Into A College Nutrition Course On Eating Behaviors, Kira L. Minot

Master's Theses

Objective: This study aimed to assess the impact of an online introductory college nutrition course that implements evidence-based intuitive eating (IE) concepts and principles on students’ application of IE behaviors. A secondary outcome explored the relationship between student grades and changes in IE behavior implementation. Researchers hypothesized an increase in the average use of IE practices post-intervention.

Methods: This study was a non-randomized pilot intervention using pre- and post-test surveys. Enrolled students completed the intuitive eating scale-2 (IES-2) survey on the first and last days of the 16-week course. Students participated in typical nutrition coursework throughout the semester with the …

Numerical Modelling Of Elastic Wave Propagation In Anisotropic Media Using The Finite-Difference Time Domain (Fdtd) And Elastodynamic Finite Integration Technique (Efit), Seth M. Golembeski

Master's Theses

As ultrasonic testing becomes increasingly widespread, education and simulation in UT becomes increasingly useful. This thesis describes development of two simulations - the first, a Finite Difference Time Domain method that can be deployed to consumer GPUs. This simulation is designed to be user-friendly and provide quick interactivity for pedagogical use, for example, in a classroom or industry training. The second simulation is an anisotropic Elastodynamic Finite Integration Technique implementation, which can be deployed to high performance computers for large-scale parallel simulations. This anisotropic simulation is, to the author’s knowledge, the first open-source implementation of anisotropic EFIT.