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Studying Milk Coagulation Kinetics With Laser Scanning Confocal Microscopy, Image Processing, And Computational Modeling, Richard Joseph Hennessy

Master's Theses

The kinetics of milk coagulation are complex and still not well understood. A deeper understanding of coagulation and the impact of the relevant factors would aid in both cheese manufacturing and also in determining the nutritional benefits of dairy products. A method using confocal microscopy was developed to follow the movement of milk fat globules and the formation of a milk protein network during the enzyme-induced coagulation of milk. Image processing methods were then used to quantify the rate of coagulation. It was found that the texture of the protein network is an indicator of the current status of the …

Characterization And Implementation Of A Decellularized Porcine Vessel As A Biologic Scaffold For A Blood Vessel Mimic, Aubrey N. Smith

Master's Theses

Every 34 seconds, someone in the United States suffers from a heart attack.  Most heart attacks are caused by atherosclerotic build up in the coronary arteries, occluding normal blood flow.  Balloon angioplasty procedures in combination with a metal stent often result in successful restoration of normal blood flow.  However, bare metal stents often lead to restenosis and other complications.  To compensate for this problem, industry has created drug-eluting stents to promote healing of the artery wall post stenting.  These stents are continually advancing toward better drug-eluting designs and methods, resulting in a need for fast and reliable pre-clinical testing modalities. …

Implementation And Assessment Of Hyperglycemic Conditions For The Creation Of A Diabetic Blood Vessel Mimic, Vikramaditya Mediratta

Master's Theses

Introduction: Diabetes Mellitus is a metabolic disorder that affects a person’s ability to either produce insulin (Type I diabetes mellitus) or properly use insulin (Type II diabetes mellitus) in order to maintain adequate blood glucose levels. The most severe diabetic complications arise due to hyperglycemia – a state of extremely high blood glucose levels – such as, coronary artery disease (CAD), in which coronary stent therapy is a popular method of treatment. However, research has shown a high rate of in-stent restenosis in diabetic patients with CAD, most likely due to activation of cellular adhesion molecules on endothelial cells exposed …

Seawater/Wastewater Production Of Microalgae-Based Biofuels In Closed Loop Tubular Photobioreactors, Joshua Bradley Lowrey

Master's Theses

The push for alternatives to petroleum fuels has forced researchers to look for highly productive, renewable, non-food resources.  The advantages of using microalgae instead of traditional oil crops for biofuel production include high oil yields, rapid reproductive rates, and versatile growing requirements.  In order to reduce the cost of producing microalgae based biofuels, wastewater has been used as a nutrient source instead of specialized plant nutrients.  The purpose of this study was to compare the relative effectiveness of different combinations of microalgae strain and dairy wastewater for increasing biomass.  The methods for monitoring growth included optical density, cell counting, biomass …

Optimization Of Culture Conditions For Equine Bone Marrow Mesenchymal Stem Cells And Their Differentiation Into Osteoblasts, Elizabeth R. Ackell

Master's Theses

The horse industry is a multibillion dollar industry where the health of the animals is of great focus for horse owners.  Bone fractures are an aspect of equine health failure that is continually catastrophic and often fatal.  In addition to discussing fracture, this review will outline the process by which bone tissue is regularly maintained and self-healed.  Also, traditional methods of healing fractures that have limited use and success will be discussed.  The lack of success that is common with the traditional methods has led to the need for additional research to identify more successful and economical ways of healing …

An Examination Of Unwritten Rule Development In Men’S Ice Hockey, Joshua M. Lupinek

Master's Theses

This research study sought to better understand at what point in the player development process did aspiring professional hockey players learn of, as well as consent to, ice hockey’s unwritten rules.  How this process occurred was also under examination.  While some research had been conducted relative to the development of unwritten rules within sport, little research to date had focused on ice hockey’s unwritten rules and none had focused on the factors associated with their development.  Research in other sport areas has indicated that knowledge and consent of unwritten rules is expected in the development of a professional athlete.  As …

Changes In Simulated 2,000 Meter Rowing Performance During 4 Years Of Intercollegiate Women’S Rowing, Katherine R. Les

Master's Theses

This study examined the performance gains in annual simulated 2,000 meter (2K) rowing tests of female collegiate rowers over their four years of collegiate-level training and competition.  Data compiled over 14 years on 47 well-trained, collegiate female rowers were used.  Subjects’ PR (personal record) 2K scores for each of their four years with the University team were analyzed.  Results showed significant differences between both Year 1 to Year 2 and Year 2 to Year 3 2K PR scores.  The greatest change in 2K scores occurred between Year 1 and Year 2.  No significant differences were seen between Year 3 and …

Comprehensive Analysis Of Effluent Organic Matter From Five Wastewater Treatment Plants In Connecticut And Comparison To Natural Organic Matter, Matthew L. Quaranta

Master's Theses

Natural organic matter is an important driver of biotic and abiotic processes in aquatic environments. Wastewater treatment plants discharge a substantial amount of organic matter into the environment; however effluent organic matter has not been well studied. In this study, traditional organic geochemical techniques were applied to characterize effluent organic matter. Effluent organic matter was isolated by DAX8 (hydrophobic fraction) and XAD4 (transphilic fraction) Amberlite resins. Extraction efficiencies of effluent organic matter by DAX8 resins ranged from 18 to 42 percent as a result of larger content of hydrophilic organic matter than natural organic matter. Average organic matter molecular weights …

Prevalence Risk For Engaging In Unhealthy Behaviors In Connecticut Adolescents Experiencing Inadequate Sleep Or Low Parental Support, Celeste F. Jorge

Master's Theses

No abstract provided.

The Decline In Baseball Participation Amongst African American Youth, Michael Mudrick

Master's Theses

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The basis for this thesis was to study the decline in baseball participation amongst African American youth through the lens of social learning theory. This was done in conjunction with interviews with 10 young African American non-baseball players, ages 11-13, who resided in an urban setting in a northeastern city. Because of the paucity of African Americans in Major League Baseball today, social learning theory, a philosophy heavily predicated on the influence of role models, was seen …

Characterization And Implementation Of Low Intensity Pulsed Ultrasound As A Tool To Apply Physical Load To Scaffolds And Bone Cells For Fracture Repair, Scott Frazee

Master's Theses

One current challenge in treating bone fractures is the effective treatment of non-unions and delayed unions. Low Intensity Pulsed Ultrasound (LIPUS) has been approved by the FDA to treat fresh fractures since 1994 and non-unions since 2000 and is an attractive treatment option because it is non-invasive. The mechanism by which it works, however, is not well understood; what is known is largely confined to the resultant changes in chemical output of cells. In this thesis several concepts and techniques were brought together to investigate the following hypothesis: LIPUS produces a measurable physical load that results in measurable deformation and …

Expression Analysis Of The Imprinted Gene Transketolase-Like 1 In Mouse And Human, Amy F. Friss

Master's Theses

Genomic imprinting is an epigenetic phenomenon resulting in differential gene expression based on parental origin. Recently, transketolase-like 1 (TKTL1) has been identified as an X-linked imprinted gene. TKTL1 functions in the nonoxidative branch of the pentose phosphate pathway (PPP), which maintains glutathione in a reduced state through the generation of NADPH. Previous studies on transaldolase, the other critical enzyme in the nonoxidative branch of the PPP, suggest that TKTL1 may affect the cell’s ability to reduce glutathione. This study provides evidence that TKTL1 overexpression inhibits glutathione reduction. Intriguingly, aberrant glutathione levels are associated with autism. Additionally, studies involving …

Modulation Of Hypoglossal Motoneurons By Nitric Oxide, Justin Philip Benoit

Master's Theses

Obstructive sleep apnea (OSA)- the occurrence of repetitive episodes of airway obstruction during sleep- is considered a major health problem affecting up to 9% of adults in the United States (Parish & Somers, 2004). The hypoglossal motor nucleus (HMN) controls genioglossus muscle tone and is critically important for maintaining airway patency; loss of excitatory input to the HMN during sleep results in disfacilitation of hypoglossal motoneurons, increased airway resistance and contributes to the development of OSA (Horner R. L., 2007). However, a fundamental question of sleep medicine that remains unresolved is what mechanisms help maintain airway patency during sleep? A …

The Zook Commission:Reassessing World War Ii Veterans' Influence On Higher Education, Jeffrey Scott Aranguena

Master's Theses

The Zook Commission: Reassessing World War II

Veterans Influence on Higher Education

Jeffrey Scott Aranguena

This thesis is an analysis of The Presidential Commission on Higher Education of 1947 and the influence World War II veterans held on the role of the Commission’s recommendations. More specifically, this thesis takes a look at the recommendations presented to President Harry S. Truman in a report titled, Higher Education for American Democracy, and the influence veterans had on the Presidential Commission’s suggestions to President Truman.  Through a major change in higher education ideology, the veterans provided an example for what higher education could …

Sustainability And The Future: A Case Study On Environmental Perspectives Of The Future Generation Of Scientists, Abby Jane Davidson

Master's Theses

In the natural sciences, countless studies have sought to quantify the environmental impacts of varying anthropogenic activities and have predicted and modeled future scenarios for the natural world under the siege of human forces. Faith for the study of and action to ameliorate environmental impacts is often put in the hands of scientists. However, little research has been conducted to look at the attitudes and perceptions future scientists have towards the environment with regard to anthropogenic environmental impacts, sustainability, and the future. Therefore, the purpose of this qualitative case study was to take an intensive look at one such group …

The Effect Of The Chemical Composition Of Low Salinity Water On The Growth, Development, And Survival Of Cultured Juvenile Blue Crabs, Dyan Patricia Gibson

Master's Theses

Cultured juvenile blue crabs ( Callinectes sapidus Rathbun) were held for up to 77 days and monitored for survival, growth and development in low salinity water containing various compositions of ions. ~rabs were isolated in separate containers and placed in thirty gallon aquaria containing 2ppt of ion solution. Temperature, ambient, humidity, and nitrite levels were monitored daily. Molts were measured to determine growth, and molt increments were recorded to determine rate of development. The first part of the study compared ratios of sodium chloride (NaCl) to commercial sea salt (SS) in five treatments: 100% NaCl, 100% SS, 25:75 NaCl/SS, 50:50 …

The New Frontier: An Analysis Of Effective New Media Political Communications And Campaigning, Kaitlin Ann Mueller

Master's Theses

Objectives: In the age of new media communication, politics has taken a swift turn as the Internet lends itself to quicker and less expensive contact. Politicians in all realms have adopted its communicative aspects in many forms as a means of promoting themselves and staying in contact with their constituents. What causes politicians to be more "connected" than others? And secondly, does that "connectivity" lead to successful elections? In this analysis, we will discuss what contributes to a United States Senator's connectivity, and if his or her heightened connectivity led to a higher probability of winning elections. Units of analysis: …

Nitric Oxide Synthesis By Chicken Macrophages Results In Coordinated Changes Of Multiple Arginine Transporters, Michael Moulds

Master's Theses

Arginine transport is primarily mediated by the cationic amino acid transporters (CATs) in mammalian cells, but in aves the y⁺, b^0,+ and B^0,+ transport systems have also been observed. Arginine is the limiting catabolic substrate required for the production of nitric oxide (NO), a highly reactive compound that acts as a signaling molecule or killing compound. NO is synthesized by inducible nitric oxide synthase (iNOS) by macrophages for pathogen clearance. In mammals, CAT-2B is responsible for ARG import in the macrophage for NO synthesis, but the chicken CAT-2B isoform does not transport ARG. Therefore the objective …

Angiostatin Like Peptides In Milk: Potential Development For Dairy Products Capable Of Cancer Prevention, Erin Stefanutti

Master's Theses

For the past 40 years, antiangiogenic approaches have been of major interest in the development of methods to cure and prevent cancer. Angiogenesis, the development of blood vessels from pre-existing vascularization, is essential for cancer growth and spread of metastasis through the delivery of nutrients and oxygen essential to sustain the metabolic activity of these malignant cells. Blocking access to blood will cause cancerous cells to assume a dormant state creating inactive micro-tumors innocuous to the host. Angiostatin, the internal fragment of the fibrinolytic zymogen plasminogen, has shown great potential in reducing cancer size and number of metastatic colonies in …

Combining Conventional Tests And Terminal Restriction Fragment Analysis To Evaluate Microbial Quality Of Raw Milk, Haibin Guo

Master's Theses

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Abstract

Combining Conventional Tests and Terminal Restriction Fragment Analysis to Evaluate Microbial Quality of Raw Milk

Haibin Guo

The dairy industry is an important part in the domestic economy in the U.S. and the quality of dairy products hinges on raw milk quality. Microorganisms play a critical role in raw milk quality and they are currently tested and monitored by conventional microbiological tests. …

Effects Of Wildland-Urban Interface Fuel Treatments On Potential Fire Behavior And Ecosystem Services In The Central Sierra Nevada Mountains Of California, Christopher C. Hamma

Master's Theses

ABSTRACT

EFFECTS OF WILDLAND-URBAN INTERFACE FUEL TREATMENTS ON POTENTIAL FIRE BEHAVIOR AND ECOSYSTEM SERVICES IN THE CENTRAL SIERRA NEVADA MOUNTAINS OF CALIFORNIA

Christopher C. Hamma

For the past several decades, the wildland-urban interface (WUI) has been expanding in the low- to mid-elevation mixed-conifer belt of California’s Sierra Nevada mountain range. Concurrently, the effects of fire exclusion and shifting climatic patterns in this region have led to increases in wildfire size and severity, posing an ever-greater risk to life and property. As a result, the need for implementation of fuel treatments to reduce fire hazard is generally recognized to be urgent. …

Characterization Of The Equine Spermadhesin Hsp-7 Found On Stallion Spermatozoa As It Relates To Stallion Fertility And Sperm Capacitation, Melodee Kathleen Heidmiller

Master's Theses

Equine spermadhesin HSP-7 is a 14 kDa protein isolated from stallion seminal plasma and present on the surface of spermatozoa. HSP-7 displays carbohydrate and zona-pellucida binding properties, but the physiological role in equine fertilization is not well defined. HSP-7 has 98% amino acid sequence homology with the well-studied boar spermadhesin, AWN. Currently, these two proteins are considered to have the same reproductive function. Immunofluorescence studies presented here show that the stallion and boar spermadhesins are localized to different segments on spermatozoa. The variation in molecular compartmentalization of spermadhesin molecules in different species suggests that these structurally related proteins could be …

High-Resolution, Non-Contact Angular Measurement System For Psa/Rsa, Ronald D. Sloat

Master's Theses

A non-contact angular measurement system for Pitch Static Attitude (PSA) and Roll Static Attitude (RSA) of hard disk drive sliders is designed and built. Real-time sampling at over 15 KHz is achieved with accuracy of +/- 0.05 degrees over a range of approximately 2-3 degrees. Measuring the PSA and RSA is critical for hard drive manufacturers to control and improve the quality and reliability of hard drives. Although the hard drive industry is able to measure the PSA and RSA at the subassembly level at this time, there is no system available that is able to measure PSA/RSA at the …

Pcr Primers For The Detection Of Propane And Butane-Oxidizing Microorganisms, Brian Jeremy Chan

Master's Theses

In an increasingly energy-hungry world, our capacity to meet the heightened energy demands of the future has become a pressing matter. The most urgent of these concerns are tied to the accessibility of petroleum. Various experts have proselytized both the imminent arrival of peak oil production rates and the ensuing decline of those rates thereafter. And to that end, the development of novel and advanced oil exploration methodologies has become almost as important as finding the sources of oil themselves.

The soils above petroleum reservoirs play host to various communities of alkane- oxidizing bacteria that can utilize the natural gas …

Laser Etched Pmma Microfluidic Chip Design And Manufacture With Applications In Capillary Zone Electrophoresis, Evan Allen Barbre

Master's Theses

This thesis encompasses a feasibility study of using low-cost materials to manufacture microfluidic chips that can perform the same functions as chips manufactured using traditional methods within an acceptable range of efficiency of chips created with more exotic methods and materials. The major parts of the project are the selection and characterization of the fabrication methods for creating the channels for fluid flow, the methods for sealing the channels to create a usable chip and the electrophoretic separations of carboxylated microspheres of different potentials. In this work we seek to answer the question if laser-etched PMMA microfluidic chips are comparable …

Sar Map Of Gel Phantom In A 64mhz Mri Birdcage By Fiber-Optic Thermometry And Fdtd Simulation, Chirag Mukesh Patel

Master's Theses

As implantable medical devices are being used more often to treat medical problems for which pharmaceuticals don’t suffice, it is important to understand their interactions with commonly used medical modalities. The interactions between medical implants and Magnetic Resonance Imaging machines have proven to be a risk for patients with implants.

Implanted medical devices with elongated metallic components can create harmful levels of local heating in a Magnetic Resonance Imaging (MRI) environment [1]. The heating of a biological medium under MRI is monitored via the Specific Absorption Rate (SAR). SAR, defined as power absorbed per unit mass (W/kg), can be calculated …

Effects Of Water Conservation On Evapotranspiration In Las Vegas, Nevada, Joseph Belli

Master's Theses

Las Vegas, Nevada, is a rapidly growing arid city that is experiencing significant challenges in water consumption. Most of the water is used outdoors, precluding recycling and reuse of water. With a large increase in population, the Southern Nevada Water Authority created the Water Smart Landscape Program to limit outdoor water usage. This study used remote sensing images and techniques to examine how much water was lost to the atmosphere through evapotranspiration (ET) in the Las Vegas metropolitan region from 1999 to 2009. The results showed that both extreme weather conditions and water policy influence ET rates.

For All Time: An Examination Of Romantic Love Through Curse Tablets, Alicia Deadrick

Master's Theses

This thesis analyzes the Greco-Roman amatory defixiones, or curse tablets, and binding spells from the fourth century BCE to the fourth century CE in order to investigate the existence of romantic love in the Mediterranean Basin. Binding spells provide a unique opportunity for cultural analysis, for they were popular with all levels of society, both genders and all sexualities. Three categories of amatory spells prevalent in ancient society (agoge, separation, and philia) undergo examination to establish romantic love as a sentiment separate from eros and philia and in opposition of the currently prevailing relationship theory of …

Differences In Eubacterial And Archaeal Soil Communities Along A Precipitation Transect In The Mojave Desert, Elaine Pressly Bryant

Master's Theses

Deserts occupy one third of the total land mass of Earth, yet little is known about microbial soil communities under conditions of low precipitation and humidity. The Mojave Desert of the Southwestern United States provides an opportunity to observe microbial communities along a precipitation transect. The diversity of these communities can elucidate which specific groups of Eubacteria and Archaea are best adapted to limited liquid water.

Two methods were utilized to determine patterns and trends of the microbial soil communities under varying precipitation levels. Viable plate counts allowed comparison of culturable microorganisms over a four-year period. Molecular techniques distinguished unculturable …

The Environmental Impact Of The Retail Photoprocessing Industry In Santa Clara County: 1996 Vs. 2006, Benoit Delaveau

Master's Theses

Consumer digital cameras appeared on the market in 1994. Consequently, the photoprocessing industry experienced a downturn beginning in 2000, with a sudden drop in activity. This study focuses on the evolution of the retail photoprocessing industry between 1996 and 2006 and evaluates its four major environmental impacts: (1) energy use, (2) associated greenhouse gas emissions (GHG), (3) silver discharge, and (4) water consumption. The main method used for data analysis is based on the ISO 14040 standard for Life Cycle Assessment. The study utilizes data from: (a) impact quantification for two photoprocessing labs in Santa Clara County, and (b) a …