Digital Commons Network^™

Determining The Intracellular Concentrations Of Flavonoids In Mda-Mb-231 Cells Using Hplc-Coupled Mass Spectrometry, Anh Dang Pham

Master's Theses

Flavonoids are polyphenolic compounds found in a wide variety of plants. In recent years, flavonoids have been found to be beneficial to human health. Our lab has investigated the ability of flavonoids to induce cytotoxicity in human breast cancer cell lines. We have discovered that some compounds can induce cytotoxicity very readily, whereas others cannot. We hypothesized that this difference is attributable to a compound's ability to transverse the cell membrane. Therefore, we used LC-MS to measure the amount of each flavonoid that is able to enter into MDA-MB-231 cells, human breast cancer cells. We tested 14 flavonoids. MDA-MB-231 cells …

Profiling Flavonoid Cytotoxicity In Human Breast Cancer Cell Lines, Sina Yadegarynia

Master's Theses

Flavonoids are part of a large family of polyphenols that are found extensively in fruits and vegetables. This class of compounds has been of considerable medical interest due to their anti-inflammatory and anti-cancer activities. Although extensive effort has been made to identify the biological effects responsible for the chemopreventive activity of these compounds, the exact molecular mechanisms involved are not fully understood. In this study, we focused on the cytotoxic effects of fourteen different flavonoids against a series of breast cancer cell lines and evaluated the induction of cell cycle arrest at G1 or G2/M phase as result of such …

Automatic Document Classification In Small Environments, Jonathan David Mcelroy

Master's Theses

Document classification is used to sort and label documents. This gives users quicker access to relevant data. Users that work with large inflow of documents spend time filing and categorizing them to allow for easier procurement. The Automatic Classification and Document Filing (ACDF) system proposed here is designed to allow users working with files or documents to rely on the system to classify and store them with little manual attention. By using a system built on Hidden Markov Models, the documents in a smaller desktop environment are categorized with better results than the traditional Naive Bayes implementation of classification.

The Effect Of Glucose On Transcription At Cpxr- And Ompr-Regulated Promoters, Andrew Charles Cosgrove

Master's Theses

While reversible acetylation of proteins has been well studied in eukaryotes and is now recognized in bacteria, global protein acetylation in bacteria is a recently appreciated phenomenon. Protein acetylation is known to affect almost every aspect of cellular physiology in eukaryotes and there is proteomic evidence that this may also hold true in bacteria. In eukaryotes, lysines are acetylated by acetyltransferases that use acetyl-CoA as the acetyl group source, and de-acetylated by deacetylases. In bacteria, this reversible process uses enzymes homologous to those used by eukaryotes.

Our lab has recently found that acetylation of RNA polymerase (RNAP) can activate transcription …

Characterization Of The Clostridium Difficile Biofilm, Michelle Laning

Master's Theses

Clostridium difficile is a Gram-positive, spore forming bacterium and an opportunistic gastrointestinal pathogen. Clostridium difficile infection (CDI) is a common nosocomial infection. Upon antibiotic treatment, patients can be colonized with C. difficile from ingestion of spores. After colonization, cells produce toxins creating symptoms from diarrhea to pseudomembranous colitis, and many patients develop recurrent infections. Interactions between host epithelium and the bacteria is a critical step in infection, yet the interaction is not well understood. It is not known how C. difficile evades host immune responses. These are important mechanisms to answer in advancing CDI treatment. A biofilm could contribute to …

(Women's) Archival Spaces And Trans Voices? A (Re)Search And Proposal, Jeremy Curtis Main

Master's Theses

Transgender has been silenced, exiled, forgotten, erased, ignored, maltreated, killed, and ultimately, in a major theme of this research project, excluded from histories. Yet, like women, African-Americans, and gay men and lesbians before them, transgender and their allies are working toward inclusion and independence. History, it seems, can no longer ignore them. One of the surest ways to "prove" a history is to have the original items of that history in an archives. So, what representation do we find among various United States' archives concerning transgender people? Unfortunately, like with many other marginalized groups, much work has to be investigated …

An Analysis Of Barriers To And Strategies For Improving Parent Engagement, Candice Renee Shakur

Master's Theses

Research shows that parent engagement can be a useful tool for improving student achievement. But, in many low-income urban schools, encouraging schools and parents to invest in parent engagement is a struggle. This paper explores some of the barriers that exist in low-income urban communities that work to limit parent engagement; it also identifies some of the strategies available to increase the frequency and quality of parent engagement in low-income urban communities.

Resveratrol + Ethanol Preconditioning And Neuroprotection, Nastaran Khodaie

Master's Theses

Our lab established that moderate ethanol preconditioning (MEP) for 6 days blocked the Aâ25-35 toxicity in cerebellar mixed cell cultures and It has been shown that MEP cause the up regulation of the pre-survival enzymes such as proxiredoxin2 (PRX2), which suggests MEP can activate the cell survival pathways against upcoming toxins.

This study was performed to verify the possible combinatory effect of ethanol and resveratrol against Aâ25-35 toxicity. Both ethanol and resveratrol sub-protective concentrations were detected against Aâ25-35 in cerebellar mixed cell cultures. The combination of sub-protective levels of ethanol and resveratrol showed the significantly reduction in number of Aâ25-35 …

Determination Of An Interaction Between Nipped B-Like Protein And Mll, Adam Robert Marek

Master's Theses

The Mixed Lineage Leukemia (MLL) protein serves as a positive transcriptional regulator during hematopoietic and embryonic development. The MLL gene can undergo chromosomal translocations producing leukemia-causing fusions that retain the MLL amino-terminus, including the repression domain. A recent yeast two-hybrid screening used the MLL repression domain as bait and yielded nine positive clones of Nipped B-like (NIPBL).

NIPBL is a crucial member of the cohesin complex, which functions in the segregation of sister chromatids during cell division. However, recent evidence suggests the cohesin complex can also function as a transcriptional regulator.

In this study, we wanted to confirm this interaction …

Invisible Margin: Marginalization And Activism Of Adivasi Women In Researches In Bangladesh, Aanmona Priyadarshini

Master's Theses

In the context of historical marginalization of indigenous people from diverse ethnic communities in Bangladesh, this study will investigate how mainstream researches and writings, in books, research reports, newspapers, and blogs on indigenous issues have ignored women's relegation by homogenizing women's distinctive problems with communities' marginalization. The research will explore indigenous women's subordinations in diverse sphere of lives and activism against the power structure that mainstream researches have overlooked. The study will also investigate the possible pathways of solidarity to challenge the systems of power that create control over indigenous people's lives. Thus this research will explore following research questions: …

Gender Inequality And Countries' Responsiveness To Enforcing Human Trafficking Laws: A Cross National Study, Christina Rose Fiorito

Master's Theses

In recent years, government agencies, advocacy groups, and academics have made attempts to address and understand the problem of human trafficking through raising awareness, conducting research and implementing prevention and intervention programs. This study tested whether gender inequality measures, which capture aspects of a country's social and political operations, are related to less governmental efforts to enforce laws against human trafficking, after controlling for other possible explanations for lax enforcement such as poverty, government corruption, political instability and increase of general violence, educational achievement, net migration, and the percent of the country's population living in urban areas.

The data were …

Self-Control As A Determining Factor In Aftercare Compliance And Recidivism Of Sheridan Correctional Center Releasees, Jana R. Krepel

Master's Theses

This study looked to Self–Control Theory to explore relationships between self–control and aftercare completion and recidivism in a cohort of Sheridan Correctional Center releases (N=604). The data set was obtained by Dr. David Olson (Olson & Rozhon, 2011) of Loyola University Chicago. Utilizing an existing inmate evaluation tool, the Client Evaluation of Self and Treatment, a new index of self–control was created, and the scales of this index became the predictor variables. After logistic regression, it was determined that none of the self–control scales were significant predictors of either aftercare compliance or recidivism. In fact, when all variables were considered, …

Muppets Abroad: Globalization Of Multiculturalism And The International Co-Productions Of Sesame Street, Julianna Carlson Palm

Master's Theses

In recent years globalization has been cited as the cause or force changing our world, making it more interdependent and "smaller" through new means of technology and communication. As such a force, globalization is seen as pervasive in all aspects of contemporary life. Sesame Street has been used as an educational tool in the United States since 1969 and since that time, the producers of the program have created a presence in various countries throughout the world, using the technology of television to communicate with preschool audiences. In particular in the last decade, new productions have begun and been funded …

Understanding The Regulation Of Metabolic Enzyme Acetylation In E. Coli, Arti Walker-Peddakotla

Master's Theses

Global protein acetylation is a newly discovered phenomenon in bacteria. Of the more than 250 acetylations reported in E. coli, many are of metabolic enzymes. Thus, acetylation could represent a novel posttranslational mechanism of metabolic control. Yet, almost nothing is known about the regulation of these acetylations or of their metabolic outcomes. Here, we report that the cAMP receptor protein (CRP) regulates protein acetylation in E. coli and provide evidence that protein acetylation modulates the flux of carbon through central metabolism. When we grew cells in mixed amino acids supplemented with glucose and cAMP, global protein acetylation increased in a …

Characterization Of The Coca Chemokine Receptor Four Agonist Activity Of Ubiquitin, Daniel M. Staren

Master's Theses

Ubiquitin has previously been identified as another natural agonist of CXC chemokine receptor 4 (CXCR4). In addition, recent evidence suggests that ubiquitin may activate CXCR4 through a binding site on the receptor, which is distinct from the binding site for the cognate ligand stromal cell-derived factor (SDF)-1α. The cellular consequences of ubiquitin induced CXCR4 activation, however, are still poorly defined and a side-by-side comparison of CXCR4 mediated functions after activation with SDF-1α and ubiquitin is lacking. Such information will be instrumental to better understand the physiological function of CXCR4 and to further define its role as a therapeutic target in …

The Influence Of Adult- Versus Child-Directed Television Programs On Distractibility In Preschoolers, Kathryn Joyce O'Toole

Master's Theses

Research in the field on children's attention to television has suggested that discerning between two different types of programming is crucial for understanding how children attend to TV. Child-directed television consists of programs designed with the purpose that children are the intended viewers. In contrast, adult-directed television is not designed for children; these programs are directed toward an older audience. The current study investigated how children divided their attention between cognitive tasks and a distractor. The distractor was either an adult-directed TV program, a child-directed TV program, or there was no distractor. The results revealed that the both distractors reduced …

When Theory Meets Practice: Challenging Racial Inequality In A Post-Civil Rights Era, Victoria Brockett

Master's Theses

Knowledge about racial inequality is important because it can inform racially just practices. To this end, multiple scholars have shown how racial inequality operates and how it can be challenged in various facets of social life. However, what does challenging racism look like when theory meets practice? Building on racial formation theory, this thesis examines a racial justice organization's (RJO) training and consulting services through the lens of a political project that is rearticulating the meaning of race and thus, the role of race in the social structure. Evidenced by observations and interviews with RJO staff and their clients, this …

Neither Butch Nor Barbie: Negotiating Gender In Women's Roller Derby, Kaley Marissa Mullin

Master's Theses

Using ethnographic methods, I began this project with the goal of understanding the full experience of what it means to be a derby girl. This included examining how the sport dictates performances of gender and how derby girls perform gender on and off the rink. Additionally, I paid special attention to how the women negotiate femininity and beauty. I found that roller derby girls maneuver through the world of gender dichotomies skillfully by means of their actions and words in the derby space. Unlike at the inception of derby, current derby girls engage less consciously with the feminist movement. Instead …

A Versatile Group Of Investigative Theater Practitioners: An Examination And Analysis Of "The Civilians", Kimberly Suzanne Peterson

Master's Theses

This thesis analyzes The Civilians, a New York-based theater company that creates interview-based cabaret/theater works, specifically examining the group's organizational structure and creative and administrative processes. The goal of this thesis is to provide insight into how this organization functions, and the lens of organizational structure and processes is used because, as business scholars have noted, structure and process are fundamental elements of any organization. Additionally, this study is framed using Gaétan Morency and Jane Needles's analysis of Cirque du Soleil, François Colbert's analysis of the Piccolo Teatro of Milan, and Celia Wren's article, "Dissolving the Barriers," which investigates eliminating …

Constructing And Deconstructing Border Wars, Damien Chadsworth Wendel

Master's Theses

Discussing a border's location, how it functions, and who is allowed to cross it can quickly give rise to heated arguments that at times escalate to violent disputes. In order to better understand the tension surrounding borders, a mediated-rhetorical critique of National Geographic's television show Border Wars was conducted. Performing this critique enables an understanding of the rhetorical strategies the show employs to construct and reify the Mexico/U.S. border, keep out outlaw discourse from influencing the civic imaginary, and answer the question, "Is there an end in sight?" This thesis will specifically address these questions: What rhetorical strategies does Border …

The Carbon Frame: Lobbying For Renewable Energy In The European Union, Kyle S. Herman

Master's Theses

This paper demonstrates how using the word "carbon" within global warming debates severely impedes lobbyists in favor of building stronger renewable energy policies in the European Union (EU). Within the EU, carbon is widely used to speak about many of the perils of climate change, global warming, energy policy, and contingent subject matters. In political circles, media outlets, and public debates, carbon acts a the pillar for many policies, discussions, and ideas related to fundamental errors of transferring energy from fossil fuels and nuclear sources. At the same time, however, limiting carbon does not necessarily preclude fossil fuels, such as …

Correlation Of Cough Frequency With Treatment Efficacy In Pulmonary Tuberculosis Patients In Lima, Peru, Jesus Gutierrez

Master's Theses

No abstract provided.

Design Of A Tetracycline Operon Inducible System For The Control Of Vaccinia Virus Replication: Implications For Vaccine Development, Caitlin J. Hagen

Master's Theses

The use of vaccinia virus (VACV) as a vaccine resulted in the eradication of smallpox in 1979. Characteristics that contribute to the effectiveness of VACV as a vaccine and viral vector include its ability to elicit strong, long-lived humoral and cell-mediated immune responses as a live-replicating virus and to accept large inserts of DNA into its genome. However, adverse events associated with its use as the smallpox vaccine have constrained it from being more widely utilized in vaccines and therapies. We propose to improve the safety of VACV as a live-replicating vector by using elements of the tet operon to …

The Impact Of Immigration Policies & Integration Programs On Multicultural Identity In Germany, Laila Bernice Schneidergossens

Master's Theses

Discussed against the background of current political developments and attitudes in Germany, the following research examines what roles immigration policies and locally implemented integration programs play in the development of immigrant identity in Germany. Further, this thesis illustrates how these policies and programs can effectively lead to a new conscious understanding of multiculturalism. Germany has created a society where the cultures of immigrants and autochthonous Germans do not intersect, thus creating separate entities that exist in a parallel reality. Immigrants face the dilemma of maintaining their ethnic sense of identity while simultaneously adapting to the cultural and political norms in …

Divergent Discourses: Development Knowledge And Malian Family Planning, Savannah A. Thompson

Master's Theses

I propose that in order to approach corrective recommendations for family planning initiatives an examination of initiative fitness is necessary. Using a Foucauldian approach, this analysis identifies the discursive formations (ideologies) at work in centralized family planning in order to elucidate the female normative model for whom initiative programs are constructed. This norm is contrasted with the lived experiences of Malian women in order to determine if the ideology dictating family planning structures is suitable for the local population. When combined with existing analyses of family planning structures and outcomes, this research helps triangulate family planning in a three-dimensional space, …

Violence Against Women In Pakistan, Amina Bath

Master's Theses

No abstract provided.

Child Soldiers: Solutions From A Humanistic Lens, Ursula Wylan

Master's Theses

This paper examines the issue of Child Soldiers using case studies of three conflicts in which children were found fighting. The paper also looks at the CSPA and the Presidential waiver of four countries by President Obama.

Human Rights Education Through Community Radio: The Promotion Of Afro-Colombian Women’S Identity And Rights, Mariana Lopez-Steele

Master's Theses

In 1991, for the first time in history, the Constitution recognized that Colombia is a multiethnic and culturally diverse country. Since then, a number of organizations have emerged to promote and reinforce the right to difference of the Afro-Colombian ethnic community, as well as to claim and defend their rights. This thesis project presents how La Red Nacional de Mujeres Afrocolombianas, Kambirí, a national network of Afro-Colombian women, has used community radio as a tool for imparting Human Rights Education to women through the promotion of their cultural identity. To accomplish this goal, I analyzed the radio programs created by …

The Path To Peace: Conflict Theory And Northern Ireland’S Troubles (1968-1998), Ruairi Wiepking

Master's Theses

This paper is a qualitative historical analysis of Northern Ireland’s Troubles. Over a period of approximately thirty years, sectarian violence in Northern Ireland dominated the headlines of newspapers in both the Republic of Ireland and the United Kingdom. Despite this violent history, Northern Ireland has enjoyed relative peace and stability since the passage of the Belfast Agreement in 1998. This paper aims to better understand why and how Northern Ireland endured a generation of brutal sectarian violence and emerged into a new era of peace and mutual understanding. In doing so, this paper incorporates theories from peace and conflict studies …

Systems At Play: The Construction Of International Systems In Social Impact Games, Jorge Albor

Master's Theses

This thesis explores how game makers conceive of and navigate the intersection between digital systems and real world systems by asking, how can social impact game designers shape procedural rhetoric to effectively address complex real world systems with digital systems? By examining three game case studies, I reach four significant findings regarding player agency, subversive play, design approaches to scale, and game difficulty in regards to systems fluency.