Digital Commons Network^™

Photo-Poetics: Photographic Narratives In The New Journalism, John David Hosemann Jr.

Master's Theses

Americans today have come to remember the unrest of the 1960s through the many shocking photographic images that emerged during that time. Images of assassinations, war, murders, and social upheaval have come to characterize this decade as particularly (perhaps perversely) photogenic. Further, these images have revealed the photographic medium as uniquely suited for confirming a violent reality that, at the time, seemed incomprehensible to an increasingly disillusioned public. In this discussion I seek to develop an understanding of photography as a theoretical process that can illuminate how we represent violent subject matter. In doing so, I apply these concepts to …

Synthesis Of Half-Loaded Nitroimidazole Indocyanine Green Dyes Attached To Carbon Nanotubes, Courtney L. Stanford

Master's Theses

Indocyanine green dye is the only FDA approved NIR fluorescent dye that can be used in humans. 2-Nitroimidazoles are very effective radiosensitizers, have low cytotoxicity, and target hypoxic tissue. By coupling the two together, hypoxic tumors can be targeted and imaged. Previous work has been done to couple 2-nitroimidazole to both sides of the indocyanine green dye. This work focuses on expanding that concept to couple 2-nitroimidazole to only one side of the dye, thus allowing for the other end to be available to couple to another compound, generating a half loaded 2-nitroimidazole indocyanine green dye. In theory, this half-loaded …

Dietary Protein Influences Paracellular Calcium Transport: Two Molecular Targets, Belinda M. Kotler

Master's Theses

The effect of dietary protein on bone health remains controversial. We have shown that increasing dietary protein increases calcium absorption in both humans and rats with no change in bone resorption. We used a rat model in which dietary protein increases intestinal calcium absorption and whole body calcium retention, to explore the molecular basis for this effect. Female Sprague-Dawley rats were fed a low (5%) or high (40%) protein diet for 7 days. On day 7, duodenal mucosa was harvested and total RNA isolated. Microarray analyses using these RNA samples identified 208 genes whose expression was at least 1.5 fold …

Phase-Resolved Characterization Of Conical Turbulent Premixed Flames: An Investigation Of Forced Blowoff Dynamics, Sayan Biswas

Master's Theses

Flame dynamics of a bluff body stabilized turbulent premixed flame as it approaches lean blowoff is of interest for practical applications. It is also important to understand the flame behavior under harmonic flow perturbations as it may occur due to acoustically unstable operation of compact combustors. In this study, a harmonically excited conical flame was studied to determine its behavior under strong burning and near blowoff conditions. Chemiluminescence imaging was employed using a Photron high speed camera to characterize the phase resolved flame characteristics for a range of excitation frequencies from 50 to 400 Hz in confined and unconfined geometries. …

"We've Got Something More To Prove Here": Racial-Ethnic And Social Class Identities And The Challenges Facing Latina/O College Students, Gretchen Marin

Master's Theses

Latinas/os are one of the fastest growing minority groups in the United States. In fact, the United States Census Bureau projects that by the year 2050, the numbers of Hispanics will more than double, increasing from the current figure of 50.5 million to 102.6 million. Despite such rapid population growth, the numbers of Latinas/os with at least a Bachelor’s degree remain low. In this study, I explore the intersection of racial-ethnic and social class identities in relation to academic identities. Specifically, I examine the challenges facing Latina/o college students of various socioeconomic backgrounds as they negotiate a predominantly White, middle-class …

Tissue Engineering A Blood Vessel Mimic While Monitoring Contamination Through Sterility Assurance Testing, Navid Djassemi

Master's Theses

Tissue Engineering A Blood Vessel Mimic While Monitoring Contamination Through Sterility Assurance Testing

Navid Djassemi

Tissue engineering blood vessel mimics has been proposed as a method to analyze the endothelial cell response to intravascular devices that are used in today’s clinical settings for the treatment of cardiovascular disease. Thus, the development of in vitro blood vessel mimics (BVMs) in Cal Poly’s Tissue Engineering Lab has introduced the possibility of assessing the characteristics of cellular response to past, present, and future intravascular devices that aim at treating coronary artery disease.

This thesis aimed at improving the methods and procedures utilized in …

Development Of In Vitro Tissue Engineered Blood Vessel Mimics In Complex Geometries For Coronary Stent Testing, Robert Dalton Chavez

Master's Theses

Coronary heart disease is the leading cause of death in the United States and occurs when plaque occludes coronary arteries. Coronary stents, which may be used to treat coronary occlusions, are small metal tubes that are implanted in coronary arteries to restore blood flow. After stent implantation, endothelial cells grow over the stent so that blood contacts the endothelial cells instead of the stent surface; this event is known as re-endothelialization. Re-endothelialization prevents blood from clotting on the stent surface and is a good predictor of stent success. Blood vessel mimics (BVMs) are in vitro tissue engineered models of human …

Characterization Of Tight Junction Formation In An In-Vitro Model Of The Blood-Brain Barrier, Michael Robert Machado

Master's Theses

Active and passive transport of substances between the microcirculation in the brain and the central nervous system is regulated by the Blood-Brain Barrier (BBB). This barrier allows for chronic and acute modulation of the CNS microenvironment, and protects the brain from potentially noxious compounds carried in the circulatory system. In-vitro modeling of the BBB has become the target of much research over the past decade, as there are many unanswered questions regarding modulations in the permeability of this barrier. Additionally, the development of a practical and inexpensive model of the BBB would facilitate a much more efficient drug development process. …

Development And Characterization Of An In-House Custom Bioreactor For The Cultivation Of A Tissue Engineered Blood-Brain Barrier, Amin Hadi Mirzaaghaeian

Master's Theses

The development of treatments for neurological disorders such as Alzheimer’s and Parkinson’s disease begins by understanding what these diseases affect and the consequences of further manifestation. One particular region where these diseases can produce substantial problems is the blood-brain barrier (BBB). The BBB is the selective diffusion barrier between the circulating blood and the brain. The barrier’s main function is to maintain CNS homeostasis and protect the brain from the extracellular environment. The progression of BBB research has advanced to the point where many have modeled the BBB in vitro with aims of further characterizing and testing the barrier. Particularly, …

Enhanced Hydrocarbon Biodegradation Using Bioaugmentation With Biowish™-Aqua Fog, Michael Robert Lehrer

Master's Theses

This study was done to determine the effectiveness of a commercially available bioaugmentation product, BiOWiSH^TM-Aqua FOG, for remediating petroleum-contaminated sandy soil. Biodegradation enhancement by BiOWiSH^TM-Aqua FOG was evaluated in laboratory microcosms by directly measuring total petroleum hydrocarbon (TPH) and indirectly using respirometry. Attempts were made to enrich hydrocarbon-degrading bacteria in BiOWiSH^TM-Aqua FOG, and the resulting enrichment cultures were screened using respirometry as well. Potential hydrocarbon-degrading bacteria in BiOWiSH^TM-Aqua FOG were isolated. Experiments were performed at bench-scale using microcosm bottles containing sand contaminated with either motor oil or No. 2 diesel fuel. The …

Like Laws And Sausages: The Tale Of A Mere Portion Of The Process To Develop The South Broad Street Corridor Plan, Amy R. Lopez

Master's Theses

The processes to develop community plans share certain standard activities and stages while remaining distinctive and without pre-scripted procedures. This study documents the process that yielded the South Broad Street Corridor Plan June 2012 draft. The objective is to present the decision-making processes and their connections to the final plan document along with the plan document itself.

Network Channel Visualizing Simulator: A Real-Time, 3d, Interactive Network Simulation Platform, Sean Michael Forsberg

Master's Theses

With a focus of always being connected, it's become typical for laptops and mobile devices to include multiple wireless network devices. Though the additional network devices have created mobility and versatility of how a user is connected, it is common for only one to be active at any given time. While likely that new mesh protocols will help maximize connectivity and power consumption by utilizing lower-power multi-hop techniques, it is still difficult to visualize these protocols due to the complexity created by each node's simple choices. Further challenges are presented by the variety of network devices which share frequency ranges …

Getting On The Bus: Marketing San Luis Obispo's Regional Transit Authority, Jenna Higgins

Master's Theses

A new trend is emerging, seeking to recognize the benefits of and encourage the use of public transportation. In the past, public transit agencies have not directed much energy or focus at marketing, seeking to use limited funds elsewhere. “The common perception is that money spent on marketing would be better spent on transit systems themselves…over time, a sustained investment in marketing increases the number of people who use transit. Increased ridership leads to increased revenue, and ideally, an increase in service to match the new demand” (Arpi, 2009).

Even as marketing gains importance in the public transit world, questions …

The Micro Check-In Framework For The Next Generation Of Location-Based Applications, Shraddha Kacha

Master's Theses

Today's mobile computing platforms provide new, convenient possibilities for location-based commercial activity. In particular, the global positioning system (GPS) provides location data for local advertising and retail activity. In this thesis, we take the next step and introduce the concept of micro check-in. Micro check-in is a simple framework that allows users to match their current location to specific objects or places at a resolution much finer than that allowed by GPS based check-in. We show that micro check-in enables customers to reveal their location at a fine level of granularity. By describing the concepts of points of service, service …

Becoming Cittaslow: A City's Journey To Becoming A Cittaslow Member, Megan Alexis Elovich

Master's Theses

The project will explore Cittaslow as an alternative to traditional urban development. Sprawl and consumption of non-local resources are discouraged with Cittaslow and preservation of culture and history become the tangible benchmarks of the community. It will explore the history of Cittaslow as a movement and an organization; as well as its influences on existing member cities and the criteria used to distinguish them from others. The City of San Luis Obispo is used as a case study to determine whether existing conditions measure up to Cittaslow criteria.

A Kinetic Study Of Anti-Vegf-A Polyclonal Antibodies And Anti-Vegf-A Ssdna Aptamers, Heather A. Hedeen

Master's Theses

A new detection reagent that could possibly augment or replace antibodies research and diagnosis methods are aptamers. Aptamers are ssDNA, RNA or polypeptide constructs that function like active antibodies. Antibodies and aptamers both specifically bind to selected target molecules, and as such they enable the detection or targeting of the presence or absence of a specific antigen.

In order to ensure that ssDNA aptamers perform similarly to antibodies, anti-VEGF-A polyclonal antibody and anti-VEGF-A ssDNA aptamer were evaluated against vascular endothelial growth factor A (VEGF-A) using Surface Plasmon Resonance (SPR). It was hypothesized that the anti-VEGF-A aptamer had the same, if …

Hard Drive Command Capture And Sequential Stream Detection, Adam David Miller

Master's Theses

This thesis explores hardware command capture as a viable means of analyzing real world hard drive usage. Hardware command capture provides insight into the IO stack where current tools fail to reach. A software platform is presented which provides trace conversion and analysis capabilities. This platform is written in Python and designed to handle traces of arbitrary size while being easily extensible for future projects to build upon. A novel Sequential Stream Detection algorithm built upon the software platform is then presented. This algorithm detects application level sequential streams and provides interesting insight into the sequential nature of the applications …

Nitrocellulose Paper Based Microfluidic Platform Development And Surface Functionalization With Anti-Ige Aptamers, Jennifer Guerin Ward

Master's Theses

The purpose of this thesis project was to demonstrate the ability to utilize the capabilities of aptamers so that they may act as capture reagents for paper microfluidic devices. Several characterization experiments were conducted as a precursor before the final experimentation was performed. Paper characterization, manufacturing protocols for printing and heating, as well as 3D chip fabrication were all performed and analyzed. The results confirmed that the control of fluid through a 3D microfluidic device based in nitrocellulose is possible.

For the biochemistry portion of this thesis report, antibodies and aptamers were chosen to react with IgE, an antibody that …

A Model Predictive Control Approach To Roll Stability Of A Scaled Crash Avoidance Vehicle, Nikola John Linn Noxon

Master's Theses

In this paper, a roll stability controller (RSC) is presented based on an eight degree of freedom dynamic vehicle model. The controller is designed for and tested on a scaled vehicle performing obstacle avoidance maneuvers on a populated test track. A rapidly-exploring random tree (RRT) algorithm is used for the vehicle to execute a trajectory around an obstacle, and examines the geographic, non-homonymic, and dynamic constraints to maneuver around the obstacle. A model predictive controller (MPC) uses information about the vehicle state and, based on a weighted performance measure, generates an optimal trajectory around the obstacle. The RSC uses the …

Prediction Of Articular Cartilage Remodeling During Dynamic Compression With A Finite Element Model, Kevin Akira Yamauchi

Master's Theses

First, an in vitro growth experiment was performed to test the hypothesis that applying dynamic unconfined compression during culture produces het- erogeneous remodeling in newborn bovine articular cartilage explants. Het- erogeneous measures of cartilage microstructure were obtained by biochemical assays and quantified polarized light microscopy. Significant differences were measured between the GAG content in the inner and outer portions of the sam- ples stimulated with dynamic unconfined compression. The COL fiber network was found to be more highly aligned in the inner portion of the sample than in the peripheral region.

Next, a poroelastic finite element model with a remodeling …

Political Feasibility Of Implementing Smart Growth Development Strategies In The Monterey Bay Area, Kristin Mckee

Master's Theses

Development over the past sixty years has created patterned growth and expansion outward from city centers, separating residences from commercial areas and employment centers. This separation of land uses has increased automobile dependency, which increases vehicle miles traveled and associated greenhouse gas emissions. California Senate Bill 375 mandates the development and implementation of a “Sustainable Communities Strategy” in order to plan regional land use and transportation in a coordinated fashion. In coordination with this effort, the Association of Monterey Bay Area Governments (AMBAG) is developing the Regional Implementation Plan for Smart Growth Development Strategies, which entails the identification of smart …

The Characterization Of Biofilm Attachment To Metal Interfaces: Effects Of Substratum Properties, Marcel D. Mendes

Master's Theses

Bacteria are among the most abundant microorganisms on earth, and can be found in essentially every environment. When a clean surface is exposed to media containing planktonic bacteria, the bacterial cells will attach to the surface and aggregate to form what is known as a biofilm. Biofilms have been shown to negatively affect many industries including medical, industrial, and food science applications. While biofilms have been well characterized from a microbiology perspective, there has been much less research from a materials science standpoint. It is hypothesized that the material properties of the substratum (such as the micro-structure) have a significant …

Preparation And Characterization Of Electrospun Poly(D, L-Lactide-Co-Glycolide) Scaffolds For Vascular Tissue Engineering And The Advancement Of An In Vitro Blood Brain Barrier Model, Deven Chandrakant Patel

Master's Theses

Developing an in vitro Blood Brain Barrier model that will replicate the physiological, anatomical, and functional characteristics of the native BBB has gained significant attention. Such a model would enable prediction of the penetration of CNS targeting drug candidates across the BBB, allow pre-screening and optimization strategies to be developed for new drugs and gene delivery formulations, and permit research groups to further understand how a dysfunctional BBB is involved in the pathogenesis of several neurological diseases.

The Tissue Engineering laboratory at the California Polytechnic State University, San Luis Obispo is currently in the process of developing a dynamic in …

A Proteomic Analysis Of Differentiation In The Mammary Epithelium, Laura Therese Strand

Master's Theses

While a great deal is known about the changing hormonal environment and the structural development of the mammary gland from pregnancy to lactation, very little is known about the molecular mechanisms governing differentiation of the mammary epithelium into a milk-secreting phenotype. It is important to acknowledge the diversity among the mammary glands of different species in order to better understand applications in human health and the dairy industry. In this study, we examined global protein expression during two states of differentiation in mammary epithelial cells from two species: in vitro proliferating and differentiated MAC-T cells (a bovine immortal cell-line), and …

The Development Of Transparent, Processable, Thermally-Responsive Coatings, Christopher David Roland

Master's Theses

Polymer matrices are commonly used as guest-host systems for organic chromophores for use in non-linear optical materials. The chromophores must be aligned or poled in an electric field in order to impart anisotropy and non-linear activity to the material. This poling process raises several issues, the two largest being the eventual relaxation of the chromophores back into random orientations due to brownian motion, and high molecular weight polymer matrices limiting chromophore mobility during poling. The prevention of this relaxation process is an area of significant interest, especially in applications that require long term coating stability and activity. In this study, …

Development And Characterization Of Phospholipid Encapsulated Quantum Dot Constructs For Biologic Applications, Laura C. Sparks

Master's Theses

DEVELOPMENT AND CHARACTERIZATION OF PHOSPHOLIPID ENCAPSULATED QUANTUM DOT CONSTRUCTS FOR BIOLOGIC APPLICATIONS

The American Cancer Society predicts that 577,190 cancer-related deaths and 1,638,910 newly diagnosed cases of cancer will occur in 2012. As these statistics show, cancer is a prevalent and devastating health issue; determined by the Mayo Clinic to be the second leading cause of death in the United States. Skin cancer is the most common form of cancer in the United States. In 2012 more than 68,000 Americans will be diagnosed with melanoma, 48,000 will be diagnosed with an early form of the disease that has not yet …

Detecting Netflix Service Outages Through Analysis Of Twitter Posts, Cailin Cushing

Master's Theses

Every week there are over a billion new posts to Twitter services and many of those messages contain feedback to companies about their services. One company that has recognized this unused source of information is Netflix. That is why Netflix initiated the development of a system that will let them respond to the millions of Twitter and Netflix users that are acting as sensors and reporting all types of user visible outages. This system will enhance the feedback loop between Netflix and its customers by increasing the amount of customer feedback that is being received by Netflix and reducing the …

Botanic Garden User Outcomes: A Means-End Investigation, Christopher Lee Wassenberg

Master's Theses

ABSTRACT

Botanic Garden User Outcomes: A Means-End Investigation

Christopher Lee Wassenberg

This study explored the outcomes that Leaning Pine Arboretum users experience from visiting the botanic garden. Understanding visitor motivations and benefits has been a focus in the field of outdoor recreation, and the subject of a number of botanic garden and green space visitor studies. Previous studies have found that visiting a botanic garden can serve as a coping strategy for dealing with and reducing life stress (Holbrook, 2010; Kohlleppel, Bradley, & Jacob, 2002; Maller, Townsend, Pryor, Brown, & St Leger, 2005) and that visiting public outdoor green spaces …

Physiological Politics: Stress And Dominance Responses To Political News, Erin Strauts

Master's Theses

No abstract provided.

Nonprofit Continuum: Brazilian-Centered Organizations In The Bay Area Of San Francisco, Caroline Rehill

Master's Theses

Brazil has always been a country of immigration. Since the mid 1980s, Brazil has also become a country of emigration. Brazilians have migrated to the United States in large numbers throughout the past decades. In the host country, Brazilians experience the same issues inherent in any immigrant community, such as immigration status, education, health, and socio-economic issues. To address these issues and ease immigrants’ stay in the host country, nonprofit organizations and community groups are created. This thesis project examines, identifies, categorizes, and contextualizes the Brazilian-centered nonprofit organizations in the Bay Area of San Francisco. Contrary to existing research on …