Digital Commons Network^™

Vaccines At The Intersection Of Religion And Politics, Charles Downey

Master's Theses

Vaccine hesitancy – a delay in acceptance or refusal of vaccines despite their availability – is a rising threat to global health and has increased in recent years in higher income countries. This is a complex phenomenon with the potential to undo decades of progress toward eradicating communicable diseases. The recent COVID-19 pandemic introduced a new wave of vaccine hesitancy, with evangelical Christians as leading actors in the movement away from vaccines in the United States. The present study investigated factors related to vaccine hesitancy and sources of health-related information among members of evangelical Christian churches in and around Hattiesburg, …

Development Of A Flow Cytometry Method For The Identification And Enumeration Of Enterococcus Faecalis, Riza Magbitang

Master's Theses

A multi-color flow cytometry method coupled with fluorescent staining was developed to detect and quantify E. faecalis concentrations in coastal waters as an alternative to the traditional membrane filtration method. The developed method was used to characterize E. faecalis during batch growth and yielded consistent results across the various physiological states of the cells. Additionally, the application of propidium iodide (i.e., a viability stain), afforded the method the ability to differentiate between cells with intact and compromised cell membranes, which raises the potential of quantifying cells that are in viable-but-non-culturable (VBNC) state. Initial field test using natural seawater samples spiked …

"Doing Their Own Thing": The Impact Of Second-Wave Feminism At The University Of Southern Mississippi, Sarah Drivon

Master's Theses

Second-Wave Feminism was largely responsible for transforming American society’s beliefs about gender roles. However, there is very little attention given to the impact that feminism had on cultural transformations in the South. Much of the historiography of feminism and women’s liberation only covers the most radical events and leaders of the 1970s, and it neglects to point out the smaller forms of resistance that ultimately made the movement successful. During this period, the South, and specifically southern campuses, had the reputation of being against women’s liberation because there was not much evidence of resistance comparable to that of northern campuses. …

Remembering, Forgetting, And Artifact Deposition In Late Prehistoric Florida, Andrew Bowen

Master's Theses

Material culture plays a fundamental role in memory processes. Artifacts are an important means of both connecting with and cutting ties with a real or imagined past. They can be reminders or symbols of connection to other people, places, things, powers, or memories, making them key components within networks and relations. The way materials are deposited or destroyed often follows specific customs or responds to environmental factors. Specific situations, like interactions with outside groups or socio-political changes, prompt particular depositional events. During the Mississippi period, such circumstances were prevalent throughout the Southeast. By treating artifacts in specific ways during deposition, …

Investigating The Response Of The Eastern Oyster (Crassostrea Virginica) To Low Dissolved Oxygen Conditions, Abiola Obafemi

Master's Theses

The Eastern oyster (Crassostrea virginica) requires dissolved oxygen (DO) of about 4 mg/L to support proper physiological function. Hypoxia and/or microxia occur when DO decreases to ≤ 2 mg/L. Salinity and temperature changes resulting from large-scale freshwater influx have been identified as key factors influencing the oyster populations in the Mississippi Sound. It is important to investigate the impacts of DO conditions on oyster mortality, biomass, size, and condition during these freshening events. Under controlled laboratory experiments, this thesis investigated 1) the response of oysters to microxic, hypoxic, and normoxic conditions 2) the response of oysters to combined hypoxia and …

Antimicrobials Produced By Ants: Chemical Properties And Microbe Specificity, Katy Chon

Master's Theses

Antibiotic resistance is a growing public health concern worldwide, and for over a decade, we have been witnessing the growth of difficult to treat infectious diseases caused by bacteria that are becoming resistant to antibiotics. This resistance can be mostly related to the misusing and improper use of antibiotics in both humans and animals. To restore this problem, humans have turned to new sources for the production of antibiotics. In this study, we focused on using ants. Social insects, including ants and bees, have faced strong disease pressures during their evolution and have developed a range of methods to fight …

Novel Antivirals Unveiled Against Acute And Persistent Coxsackievirus B3 Infection Via Rapid Screening, Maria Del Mar Villanueva Guzman

Master's Theses

Enteroviruses cause 10 to 15 million infections annually in the United States, and Coxsackievirus B3 is one of the most commonly reported. Coxsackieviruses may become persistent, characterized as a viral infection that is not cleared from host cells and that generates a continuous infection. Patients who develop persistent CVB3 infection may not respond to the same antivirals as an acute infection, which may be detrimental. Therefore, there is a need for broad-range antiviral drugs to combat acute and persistent CVB3 infection, as there is no well-accepted treatment available. We developed a model system to study persistent CVB3 using a pancreatic …

Novel Antivirals Unveiled Against Acute And Persistent Coxsackievirus B3 Infection Via Rapid Screening, Maria Del Mar Villanueva Guzman

Master's Theses

Enteroviruses cause 10 to 15 million infections annually in the United States, and Coxsackievirus B3 is one of the most commonly reported. Coxsackieviruses may become persistent, characterized as a viral infection that is not cleared from host cells and that generates a continuous infection. Patients who develop persistent CVB3 infection may not respond to the same antivirals as an acute infection, which may be detrimental. Therefore, there is a need for broad-range antiviral drugs to combat acute and persistent CVB3 infection, as there is no well-accepted treatment available. We developed a model system to study persistent CVB3 using a pancreatic …

Automated Concrete Bridge Deck Inspection Using Unmanned Aerial Systems Collected Data: A Machine Learning Approach, Rojal Pokhrel

Master's Theses

Many national infrastructures, particularly bridges, are becoming older and older day by day. Their safe operation and maintenance require routine monitoring and inspection. There are lots of bridge inspection specifications and manuals to guide inspectors to give the proper recommendations about the bridge structure and safety. These manuals and specifications require bridge inspectors to manually inspect the bridge and recommend maintenance and repair tasks referring to the specific criteria available in the manuals. In time, the application of technology makes many Unmanned Aerial Systems (UAS) advancements during the phase of data collection. For the image processing and analysis of collected …

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Exploring The Relationships Between Food Insecurity, Wic Participation, Early Life Feeding Practices, Diversity Of Dietary Exposures, And Diet Quality In The First Two Years Of Life, Vivian M. Drewelow

Master's Theses

Diet quality during early childhood is a strong predictor of mental and physical outcomes, as well as future diet quality. Although many factors determine diet quality during childhood, food insecurity is a significant predictor of lower diet quality. On the other hand, participation in nutrition assistant programs, such as the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC) is a significant predictor of higher diet quality. In addition, specific feeding practices, including timing of complementary food and beverage (CFB) introduction, types of CFB introduced early, and diversity of dietary exposures (DD), in the first year may predict future …

Spatial And Abiotic Effects Of Urbanization On Small Mammal Communities, Bri Casement

Master's Theses

Urbanization, the phenomenon of increasing human population density and artificial structures, is known to decrease the richness, abundance, and diversity of native wildlife populations, fragment natural areas, and increase human and human-commensal animal interactions with wildlife. Dynamics of species persistence in habitat fragments is often understood in the framework of island biogeography theory (IBT), with habitat fragments being analogous to oceanic islands. Despite the conceptual similarities between oceanic islands in IBT and urbanized habitat fragments (fragment size, fragment age, and distance to immigration sources), there are important differences. In urban fragments, the matrix is not completely impassible, habitats within fragments …

Characterization And Evaluation Of Bacteriophages Φpa-C And Φpa-Tc Infecting Pseudomonas Aeruginosa, Joanne Harrison

Master's Theses

Pseudomonas aeruginosa are prevalent opportunistic bacterial pathogens that cause nosocomial infections in at-risk individuals. The rise of multidrug-resistant P. aeruginosa poses an immense threat to hospitals. Additionally, P. aeruginosa are common food spoilage organisms that contribute to food waste. Alternative effective treatment methods are needed to improve public health and reduce food waste. Recent studies have shown that bacteriophages, viruses that only kill host-specific bacteria, are promising alternatives. This study characterizes two lytic P. aeruginosa phages, ΦPa-C and ΦPa-Tc, and evaluates their efficacy against P. aeruginosa in two model food systems. The morphology, genome, structural proteins, host range, growth kinetics, …

Exploring The Relationship Of Social Dominance Orientation, Symbolic Ableism, And Support For Mitigation Implementation With Exposure To A Health Threat, Samantha Chambers

Master's Theses

Social Dominance Theory (Sidanius & Pratto, 1999) proposes that groups of individuals in society are organized in social hierarchies that place dominant groups at the top of the hierarchy and subordinate, disadvantaged groups at the bottom. Research on prejudice, discrimination, and intergroup relations has largely focused on sexism and arbitrary-set hierarchies (e.g., race), leaving the idea of a health hierarchy largely neglected. Recently, Raoul (2023) presented the theoretical framework of a health-based social hierarchy, and the present study is an application of this framework. The aims of this study were to a) replicate previous findings by Raoul (2023) that found …

Digimindready: Enhancing Military Readiness Through Edge Ai-Driven Wellness, Education, And Digital Discipline Via Privacy-First Mhealth Innovation, Md Mehedi Hasan

Master's Theses

Military personnel often find themselves in intense situations that require high focus. Successfully engaging in these dangerous missions means they must efficiently control cognitive load, manage overwhelming stress, and stay focused through distractions to perform at their best. Military training significantly focuses on human performance, which benefits military readiness. The 21st century has introduced unanticipated challenges to all, such as the adverse effects of excessive screen time, external distractions, and over-reliance on technology without being aware of digital discipline. Militaries are no exception. These challenges have become an emerging threat to military personnel's cognitive, emotional, and physical well-being. On top …

Evaluating The Effectiveness Of Substance Abuse Treatment Within The Illinois Prison System And Its Effect On Recidivism, Maria Dimeglio

Master's Theses

The present study aims to evaluate the efficacy of two specific substance use disorder (SUD) treatment programs within the Illinois State prison system between July 1, 2010, and June 30, 2015. This study utilizes a quasi-experimental research design to evaluate the corrections-based Therapeutic Community (TC) programs at the Sheridan and Southwestern Illinois Correctional Centers. These programs are evaluated based on the effects they have on reoffending, specifically highlighting their effect on young individuals. The final sample used in the present study was taken from a population of 72,906 men released from the Illinois state prison system between July 1, 2010, …

Assessment Of Georgia’S Royal Catchfly (Silene Regia) Along A Forest-Edge Gradient In A Remnant Prairie Community, Megan Brooks

Master's Theses

Royal catchfly (Silene regia) is a perennial prairie forb known to grow in rocky, alkaline soils within tallgrass prairies of the eastern United States. S. regia is recorded from twelve states, including Georgia, and is now confined to fragmented habitats due to the loss of 95% of its natural environment. The species is now listed as critically imperiled in the state of Georgia. Attempts at reintroduction via outplanting have only shown limited success, necessitating more research on the environmental conditions in which this plant survives in its southern-most distribution. This study investigated the local ecological factors influencing the …

Fleeing Towards Healing: How Cam Can Heal Refugee Communities, Sarah B. Shaalan

Master's Theses

Alternative methods of healing can be found across the globe and are used by all kinds of people including refugees. This thesis will explore the potential benefits of Complementary and Alternative Medicine otherwise known as “CAM” as a supplementary strategy for helping refugees to deal with stress, Post Traumatic Stress Disorder (PTSD) and other harmful effects sustained on their journey and lives as displaced peoples. I will do so by first situating my project in the literature on Eurocentrism in healing practices and the emergence of CAM as an alternative. Then, using extensive primary and secondary sources, supplemented by an …

Neuroinflammation And Gut Barrier Function Post Traumatic Brain Injury, Anne Sullivan

Master's Theses

Traumatic Brain Injury (TBI) is a major cause of morbidity and mortality that afflicts millions of people in the United States annually. TBI mice had significantly increased expression of the pro-inflammatory cytokines and decreased expression of tight junction proteins CLDN5, CLDN12, and OCLD in brain lesion tissue. TBI mice exhibited a significant accumulation of FITC in the middle and distal part of the small intestine compared to sham mice. TBI mice exhibited an increase in pathogenic bacteria Enterobacteriaceae and a decrease in beneficial bacteria lactobacillus. TBI mice exhibited a trend toward an increase in permeability compared to no treatment. These …

The Role Of Micrornas In Lung Inflammation After Alcohol And Burn Injury, Connor O'Neal Guzior

Master's Theses

Acute lung injury (ALI) and the subsequent development of acute respiratory distress syndrome (ARDS) are among the leading causes of post-burn morbidity and mortality. MiRNAs are small non-coding RNA molecules that are known to regulate gene expression and help regulate lung homeostasis. In this study, we profiled four anti-inflammatory miRNAs (miR-146a, miR-150, miR-194, and miR-671) in lung tissue following an alcohol and burn injury. We found that miR-146a and miR-671 were significantly downregulated in lungs one day after combined injury, leading to increased lung inflammation and pathology. Biological sex did not have an effect on this pathophysiological process. We also …

Fine-Tuning Gene Expression: A Novel Therapeutic Approach For Rett Syndrome, Yewon Rhee

Master's Theses

Rett Syndrome (RTT) is a neurodevelopmental disorder that is characterized by a myriad of debilitating symptoms impacting nearly every aspect of life, including motor and gait impairment, social and cognitive deficits, as well as seizures and breathing abnormalities. RTT is caused by X-linked de novo loss-of-function mutations in the methyl-CpG binding protein 2 gene (MECP2), which is an essential epigenetic modulator. Any cure for RTT must directly target MECP2, a highly dosage-sensitive gene. This thesis developed a novel MECP2-targeted therapeutic approach using site-blocking antisense oligonucleotides to prevent microRNA-induced repression of MECP2, allowing fine-tuned control of gene expression. Here we report …

Investigating Car T Cell Persistence, Functional Phenotype, And The Associations With Clinical Response In Cd19 Car T Cell Treated Patients, Oliwia Bochnacka

Master's Theses

CAR T cell therapy is the adoptive transfer of autologous T cells genetically engineered to express an artificial T cell receptor. In the clinic, CAR T cells have been most effective in treating hematological malignancies and there have been many efforts to continue improving both the safety and efficacy of CAR T cells. Using patient blood samples from the Loyola CD19 CAR T clinical trial, we studied CD19 CAR T persistence and the cell surface phenotype to identify how CD19 CAR T cells change over time in the blood of treated patients and determine whether there were trends associated with …

Development Of An Advanced Step Counting Algorithm With Integrated Activity Detection For Free Living Environments, Paige M. Dolan

Master's Theses

Physical activity plays a crucial role in maintaining overall health and reducing the risk of various chronic diseases. Step counting has emerged as a popular method for assessing physical activity levels, given its simplicity and ease of use. However, accurately measuring step counts in free-living environments presents significant challenges, with most activity trackers exhibiting a percent error above 20%. This study aims to address these challenges by creating a machine learning algorithm that leverages activity labels to improve step count accuracy in real-world conditions. Two approaches to balancing data were used: one employed a simpler oversampling technique, while the other …

Engineering An Injectable Hydrogel With Self-Assembling 3d Vasculature, Kendyl Cohn

Master's Theses

This research developed methods for culturing self-assembling capillaries in an injectable gel as a potential method for vascularizing tissue-on-a-chip models to mimic physiological drug delivery. Additionally, a mathematical model was developed as a tool for understanding nutrient delivery and comparison of potential delivery systems. Organs-on-a-chip provide novel platforms for studying biology and physiology in 3D, allow exploration of tissue engineering on a manageable scale, and serve as models for drug screening and drug-delivery testing.

Methods were first developed for co-culture of endothelial cells and fibroblasts (3T3s or HDFs) in 2D, evaluating culture time, seeding density and ratio of HUVECs and …

Thermal Preconditioning Effects On Perforation Propensity Of Transvenous Implantable Cardiac Leads Used For Pacing And Defibrillation, Diane Muff

Master's Theses

Thermal preconditioning to simulate shipping, handling and storage conditions did not affect distal tip stiffness values for permanently implantable cardiac leads used for pacing and defibrillation. Leads that were subjected to extreme temperatures and temperature cycling did not show changes in buckle force values compared with control specimens which were maintained at ambient room conditions. Absolute differences between all measurements were small, generally under 0.05 N and were attributable to measurement variability.

Buckle force values are used to assess the propensity of leads to perforate the heart, a rare but potentially serious complication of implantable pacing and defibrillation systems. Since …

Sustaining Sustainable Farming: An Evaluation Of The Reasoned Action And Comprehensive Action Determination Frameworks For Persistence, Jet J. Tan

Master's Theses

This paper investigates the persistence of agricultural practices funded by the California Department of Food and Agriculture's Office of Environmental Farming and Innovation (CDFA OEFI). The central inquiry revolves around determining the most effective behavior model for analyzing persistence, comparing the Reasoned Action Approach (RAA), Comprehensive Action Determination Model (CADM), and CADM augmented with structural variables. The study's methodology integrates literature review, analysis of OEFI-funded practices, and statistical modeling to assess persistence levels. Contrary to existing literature, our findings reveal significantly higher levels of persistence than anticipated. Moreover, through model comparison, CADM augmented with political economic variables emerges as the …

An Investigation Into Teaching Sports Analytics, Josh Havstad

Master's Theses

Sports analytics arrived in the mainstream media through the novel and film Moneyball. However, its origins date back to operations researchers following World War II. Often considered a subdiscipline of statistics, sports analytics draws from statistics but also includes concepts from data science, communication, and marketing. As a passionate fan of sports, I have pursued statistics in my undergraduate and graduate education with the dream of working in sports for my career. However, educational opportunities in sports analytics are limited nationwide, and more specifically, there is no educational opportunity at my university, California Polytechnic State University in San Luis …

A Comparative Study Of The Npm, Pypi, Maven, And Rubygems Open-Source Communities, Saurav Gupta

Master's Theses

Open-source software (OSS) ecosystems, defined as environments composed of package managers and programming languages (e.g., NPM for JavaScript), are essential for software development and foster collaboration and innovation. Although their significance is acknowledged, understanding what makes OSS communities healthy and sustainable requires further exploration. This thesis quantitatively assesses the health of OSS projects and communities within the NPM, PyPI, Maven, and RubyGems ecosystems. We explore five research questions addressing project standards, community responsiveness, contribution distribution, contributor retention, and newcomer integration strategies. Our analysis shows varied documentation practices, insider engagement levels, and contribution patterns. Our findings highlight both strengths and different …

Design And Implementation Of A Vision-Based Deep-Learning Protocol For Kinematic Feature Extraction With Application To Stroke Rehabilitation, Juan Diego Luna Inga

Master's Theses

Stroke is a leading cause of long-term disability, affecting thousands of individuals annually and significantly impairing their mobility, independence, and quality of life. Traditional methods for assessing motor impairments are often costly and invasive, creating substantial barriers to effective rehabilitation. This thesis explores the use of DeepLabCut (DLC), a deep-learning-based pose estimation tool, to extract clinically meaningful kinematic features from video data of stroke survivors with upper-extremity (UE) impairments.

To conduct this investigation, a specialized protocol was developed to tailor DLC for analyzing movements characteristic of UE impairments in stroke survivors. This protocol was validated through comparative analysis using peak …

Matrix Approximation And Image Compression, Isabella R. Padavana

Master's Theses

This thesis concerns the mathematics and application of various methods for approximating matrices, with a particular eye towards the role that such methods play in image compression. An image is stored as a matrix of values with each entry containing a value recording the intensity of a corresponding pixel, so image compression is essentially equivalent to matrix approximation. First, we look at the singular value decomposition, one of the central tools for analyzing a matrix. We show that, in a sense, the singular value decomposition is the best low-rank approximation of any matrix. However, the singular value decomposition has some …