Digital Commons Network^™

Development Of An Attenuated Zika Virus By Editing The 5’ Untranslated Region, E. Ashley Thompson

Master's Theses

Zika Virus (ZIKV) is a mosquito-transmitted flavivirus that usually causes no symptoms to mild febrile in humans, and it has been regarded as an insignificant pathogen to public health. However, recent outbreaks of ZIKV infection have revealed that ZIKV can cause severe neurological effects in adults, such as Guillain-Barre Syndrome (GBS), and in infants whose mothers acquired the virus during pregnancy, causing Congenital Zika Syndrome (CZS). Currently, no approved vaccine is available and there is a critical need to develop an effective and safe vaccine. While most vaccine developmental strategies target the viral prM-E protein of ZIKV, we aimed to …

Neural Representation Of Articulable And Inarticulable Novel Sound Contrasts: The Role Of The Dorsal Stream, David Saltzman

Master's Theses

No abstract provided.

Custodians Of Memory: A History Of American Archival Science With Suggestions For Future Digital Preservation Efforts, Courtney Thompson

Master's Theses

The archive and the historian are symbiotically dependent on one another. The archive relies on the historian to make use of the records it houses, and the historian looks to the archive to reconstruct history. But can a historian responsibly reconstruct history when the archive is fraught with relativity and bias? This thesis serves two purposes; one, pulling from seminal archival science and collections management texts, it chronicles the monumental, intellectual changes to American archival sciences, theories, and institutions, and two, it shows how these early conversations pertaining to archival theories are both not far removed from digital preservation efforts …

Use Of Body Composition Imaging To Calculate 3-D Inertial Parameters For Inverse Dynamic Analysis Of Youth Pitching Arm Kinetics, Dalton James Jennings

Master's Theses

The objectives of this study were to 1) calculate participant-specific segment inertial parameters using dual energy X-ray absorptiometry (DXA) data (referred to as full DXA-driven parameters) and compare the pitching arm kinetic predictions using full DXA-driven inverse dynamics vs scaled, DXA mass-driven (using DXA masses but scaled centers of mass and radii of gyration), and DXA scaled inverse dynamics(ID) (using the full DXA-driven inertial parameters averaged across all participants), 2) examine associations between full DXA-driven kinetics and body mass index (BMI) and 3) examine associations between full DXA-driven kinetics and segment mass index (SMI). Eighteen 10- to 11- year-olds pitched …

Insulative (Direct Current) Dielectrophoretic Foul-Less Filtration In Microfuidic Systems, Matthew A A Whitman

Master's Theses

Filtration is a technology that is used almost ubiquitously in society from uses raging from filtration of macroparticles from water to pharmaceutical grade filtration products to remove anything larger than a protein. However, with such a wide range of uses, most filtration products have the same issue; membrane clogging (fouling) that prevents continuous use and requires frequent maintenance. This thesis hypothesizes that by applying a direct current (DC) to an insulating array of posts, they will create a foul-less insulative dielectrophoretic filter (iDEP) that does not clog since particles will levitate above the insulating array.

This thesis tested an inherited …

Electricity Price Forecasting Using A Convolutional Neural Network, Elliott Winicki

Master's Theses

Many methods have been used to forecast real-time electricity prices in various regions around the world. The problem is difficult because of market volatility affected by a wide range of exogenous variables from weather to natural gas prices, and accurate price forecasting could help both suppliers and consumers plan effective business strategies. Statistical analysis with autoregressive moving average methods and computational intelligence approaches using artificial neural networks dominate the landscape. With the rise in popularity of convolutional neural networks to handle problems with large numbers of inputs, and convolutional neural networks conspicuously lacking from current literature in this field, convolutional …

A System Architecture For Phased Development Of Remote Suas Operation, Eric Ashley

Master's Theses

Current airspace regulations require the remote pilot-in-command of an unmanned aircraft systems (UAS) to maintain visual line of sight with the vehicle for situational awareness. The future of UAS will not have these constraints as technology improves and regulations are changed. An operational model for the future of UAS is proposed where a remote operator will monitor remote vehicles with the capability to intervene if needed. One challenge facing this future operational concept is the ability for a flight data system to effectively communicate flight status to the remote operator. A system architecture has been developed to facilitate the implementation …

Patterns In Size Distribution And Catch Of Rockfish (Sebastes Spp.) In Fisheries-Independent And Fisheries- Dependent Hook-And-Line Surveys On The Central Coast Of California, Rose Elizabeth Dodgen

Master's Theses

Stock assessments are statistical models which characterize the state of a population of fish. Data for stock assessment models of West Coast nearshore groundfish come largely from fisheries-dependent sources. Incorporating fisheries-independent data would increase data availability. A potential source of fisheries-independent data which is comparable to existing fisheries-dependent data is the California Collaborative Fisheries Research Program (CCFRP), a Marine Protected Area (MPA) monitoring study. We are interested in understanding the context in which CCFRP could be implemented into assessments of nearshore groundfish, specifically rockfish. To investigate this, we used management-relevant metrics to examine three questions concerning the implementation of CCFRP …

Web Conference Summarization Through A System Of Flags, Annirudh M. Ankola

Master's Theses

In today’s world, we are always trying to find new ways to advance. This era has given rise to a global, distributed workforce since technology has allowed people to access and communicate with individuals all over the world. With the rise of remote workers, the need for quality communication tools has risen significantly. These communication tools come in many forms, and web-conference apps are among the most prominent for the task. Developing a system to automatically summarize the web-conference will save companies time and money, leading to more efficient meetings. Current approaches to summarizing multi-speaker web-conferences tend to yield poor …

Thermal Ecology Of The Federally Endangered Blunt-Nosed Leopard Lizard, Kathleen N. Ivey

Master's Theses

Recognizing how climate change will impact populations can aid in making decisions about approaches for conservation of endangered species. The Blunt-nosed Leopard Lizard (Gambelia sila) is a federally endangered species that, despite protection, remains in extremely arid, hot areas and may be at risk of extirpation due to climate change. We collected data on the field-active body temperatures, preferred body temperatures, and upper thermal tolerance of G. sila. We then described available thermal habitat using biophysical models, which allowed us to (1) describe patterns in lizard body temperatures, microhabitat temperatures, and lizard microhabitat use, (2) quantify the …

Design And Implementation Of Stewart Platform Robot For Robotics Course Laboratory, Trent R. Peterson

Master's Theses

A Stewart Platform robot was designed, constructed, and programmed for use in Cal Poly’s ME 423 Robotics: Fundamentals and Applications laboratory section. A Stewart Platform is a parallel manipulator robot with six prismatic joints that has six degrees of freedom, able to be defined in both position and orientation. Its purpose is to supplement parallel robot material covered in lecture. Learning objectives include applying and verifying the Stewart Platform inverse kinematics and investigating the Stewart Platform’s operation, range of motion, and limitations. The Stewart Platform geometry and inverse kinematics were modeled and animated using MATLAB. The platform was then built …

Residual Strength Of Franciscan-Derived Clay, Yingyi Xu

Master's Theses

In February of 2017 after a period of heavy rainfall, a slope destabilized behind Fremont Hall on the campus of Cal Poly San Luis Obispo. The geology of this slope stability failure is the Franciscan Complex. The Franciscan Complex, when weathered in place, results in clay soil that makes up the typical soil mantle on the hills throughout the region. Peak strength is the typical parameter tested to assess the strength of the soil. For the Franciscan-derived clay, the residual strength is the focus of this study to understand slope failure since the clay is the weakest portion of the …

Dynamic Procedural Music Generation From Npc Attributes, Megan E. Washburn

Master's Theses

Procedural content generation for video games (PCGG) has seen a steep increase in the past decade, aiming to foster emergent gameplay as well as to address the challenge of producing large amounts of engaging content quickly. Most work in PCGG has been focused on generating art and assets such as levels, textures, and models, or on narrative design to generate storylines and progression paths. Given the difficulty of generating harmonically pleasing and interesting music, procedural music generation for games (PMGG) has not seen as much attention during this time.

Music in video games is essential for establishing developers' intended mood …

Using Crispr-Cas9 As A Restriction Enzyme, Zack Crawley

Master's Theses

Restriction digests are a commonly utilized process for cleaving DNA at specific, but relatively common sites. Restriction enzymes have widespread use in DNA manipulation. CRISPR/Cas9 is a recently identified endonuclease which utilizes a customizable guide sequence to recognize and cut specific ~20 bp sites located in a DNA sequence. This preliminary research aimed to exploit the potential benefit of DNA restriction using the CRISPR/Cas9 procedure through alterations of different components involved in that system. We sought to refine existing CRISPR/Cas9 protocols and make a budget friendly, user-selectable CRISPR/Cas9 restriction digest protocol. The motivation for this research was to simplify and …

Molecular Weight And Heating Value Of Lignin Extracted Using Deep Eutectic Solvent., Ekugbere Owen Owhe

Master's Theses

The molecular weight of lignin helps us to determine how lignin can be applied accurately in industry. The molecular weight can predict the behavior of lignin, and how it will function when combined with other materials. This thesis discusses how the molecular weight of lignin can be found with gel permeation chromatography (GPC) after acetylation with acetic anhydride and pyridine. Different lignins gave different number average molecular weight and weight average molecular weight values, which were likely due to the biomass source, type of extraction and solubility in the solvent of choice. How the structure of lignin changed after derivatization …

Pretreatment Of Loblolly Pine Tree Toppings (Needled) Using Dess, Prathima Gujjula

Master's Theses

Contemporary industrial development and swift urbanization require environmentally sustainable energy sources. Ethanol made from biomass provides unique environmental and economic strategic benefits and can be considered a safe and clean liquid fuel alternative to fossil fuels. Ethanol’s significant advantages, such as low cost, biodegradability, and abundance, make the application of biomass for production of biorenewable energy favorable. However, biomass must be subjected to pretreatment processes to liberate the components needed for effective enzymatic hydrolysis that converts cellulose to sugars prior to fermentation to create biofuel. Production of valueadded co-products besides biofuels, through coordinated bio refinery processes, requires selectivity during pretreatment. …

Human Path Prediction Using Auto Encoder Lstms And Single Temporal Encoders, Hayden Hudgins

Master's Theses

Due to automation, the world is changing at a rapid pace. Autonomous agents have become more common over the last several years and, as a result, have created a need for improved software to back them up. The most important aspect of this greater software is path prediction, as robots need to be able to decide where to move in the future. In order to accomplish this, a robot must know how to avoid humans, putting frame prediction at the core of many modern day solutions. A popular way to solve this complex problem of frame prediction is Auto Encoder …

Using Moral Foundations Framing To Influence Partisan Attitudes Toward Latino Immigrants, Salma Moaz

Master's Theses

This study aims to use Moral Foundations Theory to reduce prejudiced attitudes towards Latino immigrants. Research has shown that liberals and conservatives tend to differ in their support of moral foundations. Liberals are more likely to endorse individualizing moral foundations (fairness and caring for others) while conservatives are more likely to endorse binding moral foundations (sanctity, loyalty, and authority). Participants read one of two brief messages in support of Latino immigrants framed in either the binding or individualizing morals. the hypothesis was that conservatives who read the binding message will have more pro-immigrant attitudes, be more supportive of pro-immigrant policies, …

The Optimum Design Of A Vacuum-Compatible Manipulator To Calibrate Space Based Ultraviolet Imagers, Jason L. Grillo

Master's Theses

Recent discoveries in geospace science have necessitated the design of compact UV imaging instruments to make space-based observations from multiple vantage points. The miniaturized ultraviolet imager (MUVI) instrument from the Space Sciences Laboratory (SSL) at UC Berkeley is under development to facilitate such discoveries on a wider scale. This thesis documents the design, integration, and characterization of a vacuum compatible manipulator to calibrate the MUVI instrument inside the UV thermal vacuum chamber at SSL. Precision linear and rotation stages were implemented with custom mounting plates to achieve four degrees of freedom. Optical components were installed to imitate the MUVI instrument …

Prediction Of Novel Antibiofilm Peptides From Diverse Habitats Using Machine Learning, Bipasa Bose

Master's Theses

Multidrug resistant bacteria often lead to biofilm formation. Biofilm is a colonizedform of pathogens (fungi, bacteria) attached to surfaces like animal or plant tissues, medical devices like catheters, and artificial heart valves. Biofilm formation prolongs the survival of microorganisms in an adaptive environment, leading to the spread of infection in different organs and causing a high morbidity rate. Given the rise of chronic infection and antibiotic resistance due to biofilm, it is essential to find an alternative solution to control biofilm infections. Antibiofilm peptides can interact with these biofilm-creating pathogens to inhibit growth, virulence, and biofilm formation. We hypothesized that …

Mobile Ka-Band Polarimetric Doppler Radar Observations Of Wildfire Smoke Plumes, Taylor Brianna Aydell

Master's Theses

Remote sensing techniques have been more recently used to study and track wildfire smoke plume structure and evolution; however, knowledge gaps remain due to the limited availability of observational datasets aimed at understanding the fine-scale fire-atmosphere interactions and plume microphysics. In this study, we present a new mobile millimeter-wave (Ka-band) Doppler radar system acquired to sample the fine-scale kinematics and microphysical properties of active wildfire smoke plumes from both wildfires and large prescribed fires. Four field deployments were conducted in the fall of 2019 during two wildfires in California and one prescribed burn in Utah. An additional dataset of precipitation …

Investigating The Nightime Departures Of Glaucous-Winged Gulls (Larus Glaucescens) And The Role Of Social Facilitation, Devon Leigh Mcclain

Master's Theses

Daytime behaviors and occupancy patterns of Glaucous-winged Gulls (Larus glaucescens) have been described and can be mathematically predicted based on environmental factors. However, little is known about the nighttime behaviors of Glaucous-winged Gulls. I used trail cameras to study the daytime and nighttime colony occupancy patterns of Glaucous-winged Gulls on a breeding colony on Protection Island, Washington, USA. Early in the breeding season gulls desert the colony en masse during nighttime even after some gulls have initiated clutches. Using acoustic recording units to identify an acoustic cue that signals the onset of the coordinated nightly departures from the colony, I …

Paleoenvironmental Implications Of Small-Animal Fossils From The Black Mountain Turtle Layer And Associated Layers, Eocene Of Wyoming, Jeremy Mclarty

Master's Theses

The Bridger Formation is an Early Middle Eocene deposit in southwestern Wyoming that preserves a rich record of life from North America. Some horizons within the Bridger Formation contain abundant fossil turtle shells, but turtle skulls are rarely found. Previous research focused on one of these fossil-rich horizons, the Black Mountain turtle layer, to develop a model for the abundance and taphonomic condition of the fossil turtles. The proposed model begins with a limestone layer deposited in a lake. Then, an influx of fine-grained volcanic ash (forming the Black Mountain turtle layer) was rapidly deposited into this lake likely causing …

The Use Of Multiple Sensory Modalities By The Antillean Manatee (Trichechus Manatus Manatus) To Locate Food In Their Natural Environments, Amanda Marie Moore

Master's Theses

Manatees are herbivorous aquatic mammals found in the coastal and inland waters of the Atlantic Ocean. All three manatee species are currently listed as vulnerable on the IUCN red list and there still remains much unknown about their ecology. It is currently unknown what sensory modalities manatees use to locate their food in the wild. A literature review of the Paenungulata clade (sirenians, proboscideans, and hyracoideans) was conducted in order to compare and contrast what is known about the sensory modalities of the clade, to better understand the sensory modalities of manatees, particularly the ones they use to locate their …

The Concept Of Truth In The Gospel Of John, Bastian Ogon

Master's Theses

Problem

The term ἀλήθεια, 'truth' is a frequently used term in the Gospel of John. The fundamental question of this paper is to discuss how truth is understood by John and if the Gospel of John conveys a specific concept of truth?

Method

The first part will briefly consider common philosophical concepts of truth, as well as epistemological issues connected to them. These approaches will be compared with John's truth concept. In the second part the most important passages where ἀλήθεια is used will be carefully analyzed exegetically, in order to understand the concept of ἀλήθεια in Johannine thought. The …

The Afterlife: An Exegetical And Theological Analysis Of Christ's Discourse In Luke 16: 19-31, Pop-Coman Ioan

Master's Theses

Problem

The parable of the rich man and poor Lazarus, in terms of its complexity and construction, is one of the most difficult decipherable parables that Jesus uttered during His time among men. With much tact, we will try to answer a question that has profound implications: Does the narrative in Luke 16: 19-31 support the existence of life after death?

Purpose

We carefully examine the Christ’s discourse in Luke 16: 19-31 in order to identify those suggestive parallels between this account of Jesus and the culture of the time, which would help us to decode more clearly both the …

Zechariah 9-14 In Light Of Allusions To The Sanctuary Motif In The Old Testament, Artur Boldt

Master's Theses

Topic

Zechariah 9-14 sheds additional light to the eschatological relevance of the sanctuary by emphasizing God’s presence among humankind. The sanctuary motif receives a vertical dimension by focusing on God’s original Sanctuary.

Purpose

Firstly, questions of introduction will be answered to give an overview of the material. In this context, the question of genre will be handled with special care. Then it will be defined how the concept of allusions is understood. This concept then will be applied to three different OT sanctuary references. Followed by an evaluation of these references. Afterwards different passages in Zech 9-14 will be examined …

The Influence Of Jesus' Approach To People Through "Teaching And Healing" On The Faith Of People In Jesus Christ And On Their Relationship To The Seventh-Day Adventist Church, Armin Danz

Master's Theses

The problem underlying this study is the slow but steady decline in membership in the Seventh-day Adventist Church in the German state of North Rhine-Westphalia. Numerous missionary efforts in the past have not been able to change this trend. However, an approach that summarizes the work of Jesus in the Gospel of Matthew has not been followed: Namely that Jesus taught people about the Kingdom of God and healed them through prayer. Both church historical sources and modern research suggest that wherever the biblical example of Jesus was followed closely it had a positive influence on the growth of churches. …

The Correlation Between Servant Leadership And Organizational Health Within The North England Conference Of The Seventh-Day Adventist Church, Carlton Hugh Douglas

Master's Theses

Problem

Statistics indicate the SDA church in the North England Conference (NEC) is having little effect on the population in the UK. This may be due to the fact that the leadership approach and the structure of the church have not changed despite globalisation, advances in technology, and increased migration. With the demands of operating within a secular, pluralistic society, the North England Conference faces a real challenge to maintain its integrity while being relevant. This study investigates the correlation between perceived servant leadership principles and organizational health by the leaders, pastoral workers, and members of the NEC.

Method

The …

Layperson's Norms Surrounding Politician Sexual Misconduct And Sexual Harassment, Yelyzaveta Distefano

Master's Theses

While there are legal definitions of what actions and circumstances constitute gender based prejudiced, sexual harassment, sexual assault, and rape, less is known about lay people's norms and perceptions of what behaviors and situations qualify as each of these categories, especially involving the role of context in which ambiguous social-sexual behaviors occur. Additionally, sexual harassment paradigms have not explored the unique power relationships of politicians and those working under them in political office, an especially topical locale of workplace SH in a post #MeToo America. 277 participants completed a survey with a 2 (setting: workplace, office party at the bar) …