Digital Commons Network^™

Lipid Quantification And Cryopreservation Of In Vitro Produced Jersey Cattle Embryos, Katherine A. Rhodes-Long

Master's Theses

Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro-produced (IVP) bovine embryos have darker cytoplasm than their in vivo-derived counterparts because of higher lipid accumulation. High lipid accumulation is associated with impaired embryo quality. Forskolin is an adenylate cyclase activator that regulates cAMP levels in cells and has been shown to induce lipolysis in IVP embryos. L-carnitine is required for transport of fatty acids from the intermembrane space of …

Containing The Blemmye: Anxiety Towards Congenital Difference In The Old English Wonders Of The East, Jessica L. Carrell

Master's Theses

This thesis aims to illuminate early medieval anxieties about sex, procreation, and congenital physical difference by applying a lens of critical disability theory to the Old English Wonders of the East, primarily as it survives in the eleventh-century manuscript, London, British Library, Cotton MS Tiberius B.v. This thesis focuses on the textual and illustrative representation of one Wonder, the Blemmye—an approximately eight-foot-tall, eight-foot-wide androgynous humanoid, whose eyes and mouth are in their chest and who does not possess a head—as a historic embodiment of what disability meant in relation to the early medieval English worldview. This thesis considers the …

Marketing To The New Generations Of Lgbtq+ Tourists, George Smith

Master's Theses

As social acceptance for LGBTQ+ people grows, it is essential to research and understand more about tourism and its important role in the lives of LGBTQ+ populations. There are tourism communities around the country that may not be familiar with this market segment and are not sure where to start. This can leave the LGBTQ+ market segment under-represented in the tourism industry. This research sets out to answer the question, “Can tourism communities who wish to expand to newer generations of LGBTQ+ travelers market to them successfully?” Mixed research methods include an exploration of city tourism websites, interviews with tourism …

Creative Expression Through The Gift Of God: The Intertwining Of Creativity And Religion Of The Poor Clares In New Orleans, Louisiana, Maureen Tierney

Master's Theses

This thesis looks at the relationship of art and creativity among the Poor Clares in New Orleans, Louisiana. Through the six sisters, the project addresses the way that religion influences the art that each sister produces. While each sister has had a different experience that drew them to religious life and their artwork, they all agree that their creativity is a gift from God, and this shapes how they engage with their art.

The literature gives an understanding to the role of the artist, and how to understand the nuance in an artist’s choice, when “why” something is produced is …

Finding Identity Through Survival: The Impact Of The Hurricanes In Zora Neale Hurston’S Their Eyes Were Watching God And Jesmyn Ward’S Salvage The Bones, Crystal F. Giles

Master's Theses

This paper attempts to convey the importance of the hurricane symbol in Zora Neale Hurston’s Their Eyes Were Watching God and Jesmyn Ward’s Salvage the Bones. In both novels, the authors use the imagery of setting combined with the characterization of poor women of color in order to emphasize both the effects of environmental disaster on vulnerable communities and also the inherent power of their protagonists to overcome systemic racism combined with natural disaster. The climax of Zora Neale Hurston’s novel revolves around the deadly 1928 Okeechobee Hurricane in Florida, and the rising action of Jesmyn Ward’s novel peaks …

The Wakefield Phenomenon: A Rhetorical Examination Of The Resurgence Of The Anti-Vaccination Movement In The 20th & 21st Century, Karen Boger

Master's Theses

This thesis explores the phenomenon of the anti-vaccination movement and existing publications documenting significant points in its resurgence in the late 20^th and early 21^st century following the now redacted publication by the former Dr. Wakefield asserting a correlation between children receiving vaccinations and children exhibiting the onset of developmental disorders, with Autism Spectrum Disorder (ASD) garnering the most public attention. With increasing numbers of parents delaying or forbidding their children from receiving vaccinations, along with the re-emergence of previously eradicated disease outbreaks and casualties, questions about the salience of Wakefield’s anti-vaccination statements arise. Investigation here is key …

The Effect Of Constant Relative Growth Rate Versus Constant Specific Growth Rate On Oocystis Sp, Rachel Kriner

Master's Theses

In this study, a strain of Oocystis sp. (S002) was grown using two different growth strategies at three different temperatures (15°C, 20°C, 25°C). One strategy (GS1) kept a constant specific growth rate (µ) and the other (GS2) kept a constant relative growth rate (µ/µ_max). In both strategies, the cultures were grown following the protocols of a hybrid growth system. The Dilution Phase is an initial semi-continuous growth phase that involves daily dilutions until set growth criteria are met. Followed by a batch culture growth protocol where the cultures were diluted at the onset of the first day and …

Evaluation Of Obturation Extrusion Of Guttacore With Varying Insertion Rates, John Barrett

Master's Theses

Abstract:

Introduction:The aim of this study is to determine whether the insertion rate of the Dentsply/Sirona Guttacore obturation product with Dentsply AH Plus sealer has an effect on 1. the extrusion amount of the obturating materials and 2. the extrusion probability of the obturating materials out of root canal apex.

Methods:Thirty root canal simulating plastic blocks were prepared with Waveone Gold nickel titanium rotary files to a size “primary” file. The prepared blocks were divided into three groups: group 1 was obturated using GuttaCore size primary obturator (analogous to the reciprocating file size) at a rate of 1 …

Assessment Of The Effects Of Malaria And Anemia In Pregnant Malawian Women Before And After Treatment Of Moderate Malnutrition, William Shipley

Master's Theses

ABSTRACT

Assessment of the Effects of Malaria and Anemia in Pregnant Malawian Women Before and After Treatment of Moderate Malnutrition

William Shipley

Background: Moderate acute malnutrition (MAM) can lead to adverse maternal and infant outcomes and possibly further complications. Supplementary foods or treatments with high quality nutrients should be administered to those with MAM in hopes to increase the chance of healthy maternal and infant outcomes. Sometimes supplementary food is not enough to overcome MAM and disease may play a role, particularly in pregnant malnourished women.

Objective: To determine if the effects of malaria and anemia moderated the effect of …

Development And Optimization Of Imaging And Image Quantification Techniques For Tissue-Engineered Blood Vessel Mimics, Ashley Turcott

Master's Theses

Blood vessels mimics (BVMs) are tissue-engineered blood vessels used to test vascular devices in an environment that mimics some simple anatomical factors of native blood vessels. It is important to accurately and consistently assess tissue-engineered blood vessels, although there is currently a lack of standardization in Cal Poly’s Tissue Engineering Lab and in the entirety of the field. The goal of this thesis was to develop and optimize imaging and image quantification techniques for tissue-engineered blood vessels.

The first aim of this thesis optimized and compared imaging and assessment techniques for electrospun scaffolds. Images from different SEMs were compared to …

Feeling Out Your Food: A Histological Analysis Of The Whisker System In Seals, Cameron Cooper

Master's Theses

The vibrissal (whisker) system is present in nearly all mammals and is especially important in deep-diving mammals. Pinnipeds (seals, sea lions, and walruses) have highly sensitive whiskers that are used to follow hydrodynamic trails created by their swimming prey. Each pinniped vibrissa is surrounded by a tripartite blood sinus system composed of an upper cavernous sinus (UCS), a ring sinus (RS), and a lower cavernous sinus (LCS). The UCS has been hypothesized to play a thermoregulatory role, insulating temperature sensitive mechanoreceptors located within the vibrissal follicle. The aim of this study was to examine this hypothesis by measuring and comparing …

Efficacy Of Catalytic Ozonation Advanced Oxidation Processes Compared To Traditional Ozone-Based Advanced Oxidation Processes For Degradation Of Recalcitrant Compounds, Cindy Jocelyn Sevilla Esparza

Master's Theses

This study reviews catalytic ozonation AOPs and traditional ozone-based AOPs to compare their efficiency for degradation of ozone-recalcitrant compounds. With the world’s population increasing and water becoming a scarce resource, it is important to improve current water recycling methods. Recycling water will play a large role in accommodating the increasing demand, but it will also be necessary to be improve the level of treatment in order to account for emerging contaminants (ECs) such as pharmaceuticals and personal care products. Advanced oxidation processes (AOPs) have been developed to degrade ECs that are not effectively removed by conventional wastewater treatment. The goal …

Fast Chirped Signals For A Tdma Ultrasonic Indoor Positioning System, Lauren M. Williams

Master's Theses

In this paper, a new concept for ultrasonic indoor positioning based on instantaneous frequency of ultrasonic signals is presented. Nonlinear phase characteristics of ultrasonic transducers introduce a frequency deviation in ultrasonic signals. By sweeping at very fast rates, a large spike in the deviation is introduced. The artefacts observable in instantaneous frequency estimations are highly localized and present an opportunity for accurate frequency detection. In order to be useful, the artefacts need to take place within the pulse and have sufficient magnitude for accurate processing. The system consists of a transducer transmitter and receiver pair, which have a center frequency …

Attacking Computer Vision Models Using Occlusion Analysis To Create Physically Robust Adversarial Images, Jacobsen Loh

Master's Theses

Self-driving cars rely on their sense of sight to function effectively in chaotic and uncontrolled environments. Thanks to recent developments in computer vision, specifically convolutional neural networks, autonomous vehicles have developed the ability to see at or above human-level capabilities, which in turn has allowed for rapid advances in self-driving cars. Unfortunately, much like humans being confused by simple optical illusions, convolutional neural networks are susceptible to simple adversarial inputs. As there is no overlap between the optical illusions that fool humans and the adversarial examples that threaten convolutional neural networks, little is understood as to why these adversarial examples …

The Strawberry Rhizosphere Microbiome: Role On Plant Health And Nutrition, Eric Michael Boyd

Master's Theses

Microbial-root associations are important to help plants cope with abiotic and biotic stressors. Managing these interactions offers an opportunity for improving the efficiency and sustainability of agricultural production. By characterizing the bacterial and archaeal community (via 16S rRNA sequencing) associated with the bulk and rhizosphere soil of sixteen strawberry cultivars in two controlled field studies, we explored the relationships between the soil microbiome and plant resistance to two soilborne fungal pathogens of strawberry (Verticillium dahliae and Macrophomina phaseolina). Overall, the plants had a distinctive rhizosphere microbiome relative to the bulk soil, with higher abundances of known beneficial bacteria …

Barriers To Success: Sheep And Goat Producers In The Service-Grazing Industry, Erin A. Campbell-Craven

Master's Theses

Service-grazing is a novel term for grazing done on land not owned or rented by a livestock producer or manager, for the purpose of land management, and for which the owner or land manager receiving grazing services pays compensation to the service provider. This research project seeks to gather detailed information about producers in the Western United States, providing grazing services under this project’s definition of “service-grazing”, with a focus on those operating in California, in order to discover the business models or practices necessary to be successful within a service-based grazing operation. To this end, an online survey consisting …

Pier Portal, Alec W. Hardy

Master's Theses

The Pier Portal marine monitoring system is an internet controlled underwater camera that will enable students, researchers, and the general public the ability to monitor underwater marine life and ocean conditions. Installed at Cal Poly’s Center for Coastal Marine Science pier at Avila Beach, California, the camera can be commanded in real-time to raise or lower to any position between the top of the pier and the bottom of the ocean floor, while providing a live-stream video to the operator and general public. This thesis effort focused on the integration of the various subsystem components through software, and the development …

Planning For Pollinators, Encouraging Biodiversity For Agriculture Resiliency, Sebastian Brian Cardoza

Master's Theses

Pollinators are essential to food production globally. Without them, the production of food becomes increasingly difficult, time consuming, and costly. Unfortunately, due to the rapid changes to our earth's physical and climatic structure millions of species are becoming classified as threatened, endangered or extinct. Among these declining species are pollinating insects such as Aphis mellifera (honeybee) and other native species. This is due to a phenomenon known as colony collapse disorder (CCD) and other native bees. The decline indicates that the future habitability of earth may be in jeopardy. The solution to this crisis is to expand the habitat of …

Reduction Of Fat Content In Processed Meats Using Hot-Boning And Cold-Batter Mincing Technology, Morgan P. Wonderly

Master's Theses

Processed meats have received negative publicity due to high fat contents that have been linked to adverse effects on human health. Fat is an essential ingredient in many processed meat products, so reducing this all while maintaining the desired characteristics of the product is a challenge. The purpose of this study was to generate low-fat meat products using a combination of hot-boning/crust-freeze-air-chilling (HB-CFAC) and cold-batter mincing (CBM) technologies. Pork hams were subjected to HB-CFAC or chill-boning/crust-freeze-air-chilling (CB-CFAC) prior to 3 min pre-mincing and 6 min mincing for control gels with back-fat addition or low-fat gels with water addition instead of …

The Influence Of The Sensory Environment On Avian Reproductive Success And Human Well-Being, Danielle Marie Ferraro

Master's Theses

Sensory pollutants such as anthropogenic noise and night lighting now expose much of the world to evolutionarily novel sound and night lighting conditions, which can have detrimental effects on humans and wildlife. In my first chapter, we exposed wild Western Bluebird (Sialia mexicana) nestlings to noise, light, and combination (i.e., noise and light) treatments. Nests exposed to noise and light together experienced less predation than control and light-exposed nests, and noise-exposed nests experienced less predation than control nests, yet overall nest success was only higher in noise-exposed nests compared to light-exposed nests. Although exposure to light decreased nestling body condition …

Maturation And Culture Media Effects On In Vitro Bovine Embryo Developmental Competence, Ciara M. Helland

Master's Theses

In vitro produced bovine embryos are critical to the cattle industry. However, these embryos have altered morphology, epigenetics, and metabolism when compared to their in vivo counterparts. The aim of this thesis was to alter maturation and culture media to improve the developmental competence of in vitro bovine embryos. This thesis is comprised of three experiments and one proof of concept study. Each experiment followed the same general layout: oocyte aspiration from Jersey or Holstein ovaries, oocyte maturation for 24 hours, fertilization with bull semen for 24 hours, then embryo culture for 7-8 days in 38.5 °C in 5% O …

Estimating Evapotranspiration Of A Riparian Forest Using Sap Flow Measurements, James R. Solum

Master's Theses

To close the water use budget of irrigated agricultural fields in floodplains with substantial riparian corridors, it is necessary to understand groundwater usage by dominant phreatophytic vegetation, particularly when the primary source of water for irrigation comes from groundwater abstraction. We report here results of estimated evapotranspiration (ET) of a riparian forest, which were based on measurements of sap flow in phreatophytic vegetation within a riparian corridor. The riparian corridor was within a study area 75 to 140 meters wide in the lower portion of the Scotts Creek watershed, which is bounded to the west by the Pacific Ocean in …

Design Of Prototype Prosthesis For A Canine With A Right Front Limb Deformity As An Alternate Approach To Stabilize Gait And Withstand Gait Forces, Tayler R. Kastlunger

Master's Theses

Congenital and developmental limb deformities in canines are rare and can occur as a genetic disorder or be caused by extrinsic factors. Without surgery to correct the deformity, conservative management can be implemented to manage exercise and restrict high-intensity activity of the canine. However, any alteration to the normal gait and locomotive biomechanics of a canine can have significant long-term effects on the musculoskeletal health and quality of life of the canine. To improve quality of life and provide an alternative and more cost-effective approach to surgery, a custom prosthetic was designed and developed for a canine born with a …

Bootstrapping Massively Multiplayer Online Role Playing Games, Mitchell Miller

Master's Theses

Massively Multiplayer Online Role Playing Games (MMORPGs) are a prominent genre in today's video game industry with the most popular MMORPGs generating billions of dollars in revenue and attracting millions of players. As they have grown, they have become a major target for both technological research and sociological research. In such research, it is nearly impossible to reach the same player scale from any self-made technology or sociological experiments. This greatly limits the amount of control and topics that can be explored. In an effort to make up a lacking or non-existent player-base for custom-made MMORPG research scenarios A.I. agents, …

The Yanks Are Striking: Kern County, The 1921 Oil Strike And The Discourse On Americanism, Peter F. Hussey

Master's Theses

In the fall of 1921 oil workers of the San Joaquin Valley faced a post-war economic slump, wage cuts across the board and an increasingly hostile attitude of oil operators towards consultation with the federal government on labor relations. They voted to strike, and the next day eight thousand workers walked off the fields. Strikers crafted an image of “patriotic unionism,” underpinned by a faith in the federal government and the ideology of the American Legion. The strike did not end in gruesome class warfare like had been seen months earlier in the coal mines of West Virginia, but rather …

An Examination Of Garden-Based Education Impacts On Title 1 Elementary School Students’ Environmental Literacy In San José, California, Alexandra Dahl

Master's Theses

The objective of this research was to assess garden-based pedagogy as an effective strategy to increase environmental literacy in elementary-aged students. With severe environmental issues facing children today and into the future, environmental literacy is a desired outcome of environmental education programs. Such interventions create environmentally responsible citizens who understand how humans impact the natural environment and thereby act sustainably. Currently, little research exists on garden education’s influence on students’ environmental literacy, especially in underrepresented communities. Using a nonequivalent control group study design, this project investigated the impact of a garden education program in Central San José, CA in an …

Whiteness: An Ideology Of Violence And Power As Represented In Chesnutt's The Marrow Of Tradition, Schuyler's Black No More And Larsen's Passing, Keith Giles

Master's Theses

The purpose of this thesis is to investigate the representations of whiteness found in three pieces of literature. The thesis not only teases out the nuances of whiteness, but specifically analyzes the violence that whiteness inflicts upon the black body. To begin, I define what whiteness and whiteness ideology is in relation to race. After introducing the idea and functions of whiteness, I dive into the investigation by analyzing three major novels written by African American authors during the early twentieth century. While analyzing the instances of white violence in one of the three novels, the other two novels will …

Understory Response To Restorative Thinning In Coast Redwood Forests, Alyssa Hanover

Master's Theses

Restoration of late seral features in second-growth Sequoia sempervirens forests is an important management concern, as so little of the original old-growth remains. The abundance of herbaceous species provides a metric for assessing recovery in these forests where associated understory plants are adapted to closed canopy conditions. Data were collected using a stratified random sampling design across three treatments: mechanically thinned second-growth, un-thinned second-growth, and old-growth reference stands. Pairwise PerMANOVA indicated greater abundance and diversity of native herbs in old-growth and un-thinned second-growth compared to thinned stands, as well as higher measures of canopy cover, basal area, and dominance of …

Using Stable Isotopes To Determine Foraging Areas Of Leatherback Sea Turtles: Limitations Of The Isotope Tracking Technique In The Western Atlantic Ocean, Sharon Hsu

Master's Theses

Reproductive output has long been linked to habitat quality and resource availability. Individuals foraging in high-quality habitats with high resource availability will have better body conditions and higher survival rates, as well as greater reproductive output. Post-nesting, Western Caribbean leatherback turtles are known to migrate to at least two foraging regions: the western North Atlantic and Gulf of Mexico. This study had three objectives: [1] measure δ13C and δ15N values in bulk skin tissue of females nesting in Parismina, Costa Rica to reveal prior foraging region; [2] assess influence of foraging region on female body size and reproductive output; and …

All Things Come To Pass, Brandon Luu

Master's Theses

All Things Come to Pass is a poetry collection consisting of 36 poems that revolve around the themes of impermanence, death, and memory. It is divided into three sections: Crisis, Melancholy, and Acceptance, inspired by the five stages of grief by Elisabeth Kübler-Ross. The poems are written primarily in free-verse and include genres such as elegy, pastoral, and narrative. The collection contemplates whether given the finite nature of life, in an existence where nothing lasts forever and everything seems ultimately inconsequential, what is it that should be considered important?