Digital Commons Network^™

Through The Eyes Of Community Educators: Place-Based Learning, Equitable Access, And Covid-19 Impacts On Outdoor Environmental Education In The San Francisco Bay Area, Alycia Ellington

Master's Theses

Not all children receive adequate education, nor do they have the opportunity to go beyond the four walls of a classroom for learning opportunities. Outdoor environmental education with place-based learning frameworks aims to fully immerse students in nature, while strengthening the connection between schools and communities. This study examines the outdoor environmental education programs of nine agriculture-centered community organizations in the San Francisco Bay Area. Through twenty-one key informant interviews, I assess three essential areas: 1) promotion of environmental literacy and place-based learning, 2) facilitation of equitable access to educational programs, and 3) adaptation of those programs for distance learning …

Options For Low-Cost Manufacturing And Safer Cell Therapies, Melis Keceli

Master's Theses

The proposed work aims to overcome the economical and feasibility-related limitations of the chimeric antigen receptor therapies by developing an artificial cell signaling pathway whose design transforms K562 cells into in vivo living vectors to synthesize therapeutic proteins upon engaging diseased cells in the treatment of ovarian cancer. There are various advantages of using K562 cells throughout this process. First, Food and Drug Administration approves the reinfusion of K562 cells into patients’ bodies. Second, K562 cells are more affordable than T lymphocytes, and finally, these cells can be easily manipulated with any desired genetic material and can keep the expression …

When God Gets Angry: Towards A Theology Of Divine Wrath, Lukas Daeubler

Master's Theses

Topic

One of the most significant concerns regarding the character and love of God is God’s wrath, especially in the Old Testament. For many, it is impossible to believe in a God who punishes and kills rather than a God who shows compassion and love for all people. They neglect either the violent parts of the Bible, since they seem to contradict the image of a loving God displayed in the Gospels. This of course directly questions the integrity of God and His word. As a response, some theologians have published papers on this topic, but many Christians and denominations …

The Removal Of Sin In Second Temple Jewish Literature, Odiaka Walker

Master's Theses

Problem

This thesis explored the Second Temple literary background of the concept that Jesus appeared at the end of the ages to take away sin, in Heb 9:26. As such, the research question which guided the paper was: what did Old Testament and Intertestamental writers believe about the removal of sin at the end of the ages? The answer to this question infers other questions: who would remove sin? From where would sin be removed? How was sin defined? How would sin be removed? When would sin be removed (are there time specifications involved)?

Method

The terms ἀφαιρέω, ἀφίημι, ἀπαλείφω, …

Investigating The Function Of Carboxypeptidase O Gene Paralogs From Xenopus Tropicalis, Ritchie Saint Jean

Master's Theses

Carboxypeptidase O (CPO) is a peptidase that is a member of the A/B subfamily of the metallocarboxypeptidases. This peptidase has been found in the brush border of the intestine and has specificity toward C-terminal acidic residues. CPO has been proposed to be involved in intestinal digestion and the formation of lipid droplets. Four copies of the CPO gene are present in the Xenopus tropicalis (tropical clawed frog) genome and are noted as CPO.1, CPO.2, CPO.3, and CPO.4. Not much is known about these genes regarding their properties and functions. In my research, I sought to investigate the properties and functions …

Characterizing A Novel Cocksackievirus B3 Protease Mutant And Its Response To Polyamine Depletion, Bridget Hulsebosch

Master's Theses

Enteroviruses, including Coxsackievirus B3 (CVB3), are pervasive pathogens that cause significant disease, including cardiomyopathies. Unfortunately, no treatments or vaccines are available for infected individuals. We identified the host polyamine pathway as a potential drug target, as inhibiting polyamine biosynthesis significantly reduces enterovirus replication in vitro and in vivo. Here, we show that CVB3 is sensitive to polyamine depletion through the polyamine analog diethylnorspermidine (DENSpm) which enhances polyamine catabolism through induction of polyamine acetylation. We demonstrate that CVB3 acquires resistance to DENSpm via mutation of the 2A protease, which enhances proteolytic activity in the presence of DENSpm. Resistance to DENSpm occurred …

The Gendered Impact Of Droughts On Household Decision Making: Evidence From Uganda, Fiona Onyango, Andrew Hobbs

Master's Theses

Do female headed households lose/ sell more of their livestock in the event of a drought as compared to households headed by males? We address this question through an analysis of the combination of Living Standards Measurement study Integrated surveys on Agriculture dataset on Uganda using data from the period 2011-2016 and NDVI & Weather data. We carry out both OLS and fixed effects estimations. Our OLS Results are consistent with the theory of Consumption smoothing while we do not find evidence of any gendered effects from both the OLS and IV estimations.

Plasmodium Tlap-2 Is A Microtubule-Associated Protein With Microtubule Stabilization Properties, Grifin Berge

Master's Theses

Microtubules facilitate major cellular and morphological changes in eukaryotic cells, including the protozoan parasite Plasmodium. Microtubule-associated proteins (MAPs), only very few of which have been characterized in the malaria parasite, regulate the highly dynamic microtubules. Thioredoxin-like associated protein 2 (TLAP2) is a MAP unique to the phylum apicomplexa, which in addition to Plasmodium includes the important human pathogen Toxoplasma. Here we report on the cloning and microtubule-binding activity of TLAP2 of the rodent malaria parasite Plasmodium berghei (PbTLAP2). PbTLAP2 is coded by a single exon gene and consists of 598 amino acids. The protein contains a putative C-terminal microtubule-binding domain. …

The Identification Of Lxr-Dependent Pathways In The Dorsal Root Ganglia In Models Of Obesity, Nadia Elshareif

Master's Theses

Peripheral Neuropathy is characterized as injury to the peripheral nervous system. In terms of sensory neuropathy, metabolic syndrome is involved in chronic inflammation and ER stress pathways induced by lipid overload, leading to the structural and physiological damage of afferent sensory nerves. To protect the cell from lipid-induced ER stress, Liver X Receptors (LXRs) are sterol-activated nuclear transcription factors that are of particular interest due to its role in the regulation of lipid homeostasis, membrane phospholipids, and inflammation in metabolic tissues. Their role in the peripheral nervous system remains to be elucidated. LPCAT3, regulated by LXRs, has been shown to …

Elucidating Regulatory Connections Between The Second Messenger Cyclic-Di-Gmp With Biofilm And Motility Phenotypes Of Vibrio Fischeri, Ali Razvi

Master's Theses

The symbiosis between the gram-negative marine bacterium Vibrio fischeri and its host Euprymna Scolopes occurs when the bacteria reaches and colonizes the light organ. This pathway to colonization begins in the outer marine environment and finishes within the deep crypts of the light organ, with states of biofilm formation and dispersal in between. This switch between biofilm formation to dispersal is important as the bacteria transitions from the surface on the light organ into a pore leading to the interior of the light organ. This dispersal is regulated by LAP system which is dependent on the second messenger c-di-GMP. Through …

Advancing Our Understanding Of Mechanosensitive Microtubule Acetylation In Neurons, Margaret Earl Utgaard

Master's Theses

Stiffness is a signaling cue in the brain, and neuronal response to stiffness impacts development, injury and disease. Mechanotransduction is the process of cells interpreting and converting mechanical cues to biochemical signals. Tubulin acetylation increases microtubule stability and could be mechanosensitive. The impact of stiffness on tubulin acetylation and role of septins is the focus of this project.It was hypothesized that stiffer surfaces would increase tubulin acetylation. SH-SY5Y cells were plated on varying stiffness and the ratio of acetylated to alpha tubulin was measured using immunocytochemistry. This study found SH-SY5Y cells had the highest acetylation on hard surfaces. SH-SY5Y cells …

Characterization And Targeting Of Voltage-Gated Ion Channels In Meningioma Cell Lines, Erik White

Master's Theses

Meningiomas (MN) are primary brain tumors originating in the meningeal covering of the central nervous system. These tissues can evolve through familial inheritance (NF2, Cowden Syndrome, or Werner Syndrome) or sporadic mutations. The WHO categorizes MNs into three grades (I-III); Grade I contain benign tumors and Grade III the most aggressive. Increasing grade coincides with an increase in genetic diversity, making universal treatments difficult. MNs are typically treated with hydroxyurea (HU), but this chemotherapy agent has many adverse side effects, leading to research in less toxic options. This study uses ion channel antagonists for T- and L-type calcium channels using …

Pseudomonas Aeruginosa Prophages And Engineered Temperate Phages, Genevieve Johnson

Master's Theses

The rising rate of antibiotic-resistant bacteria is a global health concern, and the Pseudomonas aeruginosa is estimated to be a leader in serious threats. Bacteriophages, or viruses that infect bacteria, have gained renewed interest in Western Medicine as an alternative to antibiotics. Phage therapy of P. aeruginosa infections has primarily focused on obligately lytic phages. However, prophages, or phages in the lysogenic life cycle, are estimated to far outnumber obligately lytic phages. In theory, these prophages can be genetically modified to be obligately lytic and thus ideal candidates for phage therapy. To date there has not been a comprehensive analysis …

Rethinking Intimacy: Liberation Through Decolonial & Queer World-Making, Michelle Mae Mcguire

Master's Theses

Relationships play an important role in both the private and public spheres of our lives. If we understand our bodies to be the vessels through which we interact with all other objects, we come to understand the process of world-making as a summation of our relationships. Intimacy is the prevailing structure that helps assign meaning to these relationships. Intimacy binds together unfixed spatial and social relations that stretch across time and space. This essay examines the three intersecting sets of relations involved in intimacy as a means to deconstruct heteropatriarchal order and highlight the multiplicity of attachments and relationships that …

The Effect Of Gonadal Steroids Following Repeat Mild Traumatic Brain Injury On Anxiety-Like Behaviors In Rats, Trevor Nykamp

Master's Theses

Traumatic brain injury (TBI) affects millions of people in the U.S. each year. This thesis worked to address anxiety, a common emotional symptom following TBI, by utilizing a five-hit repeat mild TBI (rmTBI) experimental model. First, changes in gonadal status following injury and the severity of anxiety symptoms experienced were investigated. Our data revealed that suppressed testosterone levels following rmTBI resulted in significantly more anxiety-like behaviors across several observational variables. Even more, some of these behaviors were reduced by providing testosterone supplementation immediately following injury.Another goal of this work was to investigate the role of mitochondrial dysfunction, measured by COX4i1 …

A Role For Notch Signaling In Drosophila Spermatogenesis, Adrianna Soriano

Master's Theses

The Notch signaling pathway has diverse functions in male and female gonad development. In Drosophila male embryos, Notch specifies hub cell fate in somatic gonadal precursor cells (SGPs), which are important for maintaining germline stem cells and somatic cyst stem cells throughout the lifetime of adult flies. However, a role for Notch signaling in adult males has only started to emerge recently. Nonetheless, we still don’t understand how the Notch pathway functions to regulate spermatogenesis. Notch has been established to be important for the fertility of multiple systems, including mammals, and must be properly regulated for proper spermatogenesis, but the …

Anthropogenic Litter In Illinois Streams: Monitoring, Distribution, And Relationship To Biological Indicators Of Stream Health, Lauren Wisbrock

Master's Theses

Anthropogenic litter (i.e., trash; AL) is increasing in aquatic ecosystems worldwide. AL shows a patchy, uneven distribution in lotic ecosystems due to heterogeneity in its sources, how it moves, and mechanisms of retention. In addition, the diversity of material types, size, and mobility makes developing techniques to measure AL assemblage in rivers a challenge. In rivers, watershed land-use and riparian features likely impact AL abundance and composition, but this impact is not fully understood. Measuring AL can be time consuming and labor intensive, so rapid assessments are needed for ecosystem managers to quantify the level of AL impairment in a …

Detecting Feeding And Estimating The Energetic Costs Of Diving In California Sea Lions (Zalophus Californianus) Using 3-Axis Accelerometers, Mason Russell Cole

Master's Theses

Knowledge of when animals feed and the energetic costs of foraging is key to understanding their foraging ecology and energetic trade-offs. Despite this importance, our ability to collect these data in marine mammals remains limited. In this thesis, I address knowledge gaps in both feeding detection and fine-scale diving energetic costs in a model species, the California sea lion (Zalophus californianus). I first developed and tested an analysis method to accurately detect prey capture using 3-axis accelerometers mounted on the head and back of two trained sea lions. An acceleration signal pattern isolated from a ‘training’ subset of synced video …

A Silicon Valley Life: A Silicon Valley Love Story, Sharon Simonson

Master's Theses

In the last three decades, Silicon Valley has become one of the world’s most watched and imitated communities. Daily news reporters, long-form journalists, cinema and television-programming producers have crafted a public image, but it represents nothing of the lives of hundreds of thousands of Valley residents. These fabrications obscure and diminish our complex human profile and reduce our uniquely beautiful geography to a place to generate financial profits, with all the damaging disregard such attitudes foster. The essays of A Silicon Valley Life: A Silicon Valley Love Story seek to show our true self: our rich mix of people drawn …

Lstm-Enabled Level Curve Tracking In Scalar Fields Using Multiple Mobile Robots, Kunj J. Parikh

Master's Theses

Autonomous mobile sensor networks are ideal candidates for exploring large-scaleunknown fields with tasks ranging from source seeking, level curve tracking, mapping an unknown field, and many more. In this work, we investigate the problem of level curve tracking in unknown scalar fields using a limited number of mobile sensors. The level curve tracking problem has been studied in many applications such as monitoring the propagation of fire boundaries and the algae blooms. We design and implement a long short term memory (LSTM) enabled control strategy for a mobile sensor network to detect and track the desired level curve. We develop …

Noted Mirrors, Dennis Spears

Master's Theses

Noted Mirrors seeks exploration, it seeks discovery it seeks experience. I think, like most of us, I’ve often had far more wonders than answers, but I often like it that way. Noted Mirrors tries to understand a romance that frightened, a friend taken away too soon that crippled. An unconditional love from grandparents and a mother that rescued. A relationship with an absent father that poisoned. Revelational relationships with familiar strangers that taught so much in the form of freeing. A fascination with Redwoods and Orcas that brought so much life into merely being. And a curiosity of the abstract …

Envrment: Investigating Experience In A Virtual User-Composed Environment, Matthew Key

Master's Theses

Virtual Reality is a technology that has long held society's interest, but has only recently began to reach a critical mass of everyday consumers. The idea of modern VR can be traced back decades, but because of the limitations of the technology (both hardware and software), we are only now exploring its potential. At present, VR can be used for tele-surgery, PTSD therapy, social training, professional meetings, conferences, and much more. It is no longer just an expensive gimmick to go on a momentary field trip; it is a tool, and as with the automobile, personal computer, and smartphone, it …

Real-Time Body Tracking And Projection Mapping In The Interactive Arts, Sydney Baroya

Master's Theses

Projection mapping, a subtopic of augmented reality, displays computer-generated light visualizations from projectors onto the real environment. A challenge for projection mapping in performing interactive arts is dynamic body movements. Accuracy and speed are key components for an immersive application of body projection mapping and dependent on scanning and processing time.

This thesis presents a novel technique to achieve real-time body projection mapping utilizing a state of the art body tracking device, Microsoft’s Azure Kinect DK, by using an array of trackers for error minimization and movement prediction. The device's Sensor and Bodytracking SDKs allow multiple device synchronization. We combine …

Missing In Action: Locating The Body In Interdisciplinary Studies, Ann Lenore Moradian

Master's Theses

This research project relied on the use of interdisciplinary literature, surveys and interviews to explore the prevalence of the human body and its movement across a variety of disciplines other than movement-based practices, and also to explore perspectives of movement experts regarding the knowledge, skills, and capacities they believed might be related to their practice-based work. In other words, what do movement practitioners and movement experts know, and who cares? The materials gathered were analyzed with the aim of understanding the range of possibilities for future work, and identifying areas of potential discourse, engagement, or collaboration across disciplines. The outcomes …

Fostering Resilience: Integrating Positive Character Strengths With Dance Making Skills To Help Young Children Cope With Adversity, Laura Vormwald

Master's Theses

The purpose of this study was to investigate how involvement in a choreography unit focused on positive character strengths could increase confidence in strengths that foster resilience. Research shows that building resilience is the key to helping children overcome adversity and trauma. Both qualitative and quantitative methods were used to answer the three research questions in this study. Q1 Does involvement in dance making build students’ character strengths? Q2 Which character strengths are best developed through dance making? Q3 Which inspirations for dance making relate most thoroughly to the selected character strengths? The research instruments used were a pre and …

Development And Use Of An Environmental Dna Tool To Monitor Recovery Of The Critically Endangered Smalltooth Sawfish, Pristis Pectinata, In The Northwest Atlantic, Ryan N. Lehman

Master's Theses

The Critically Endangered Smalltooth Sawfish, Pristis pectinata, was once common in the tropical and subtropical waters of the Atlantic Ocean; however, following global declines in range and abundance over the past century, individuals were restricted to the waters of south and southwest Florida (SWFL) by about the 1980’s. Recently, public encounter reports have emerged in historically occupied habitats in United States waters, suggesting individuals are present in, or re-occupying, these areas, although the status of P. pectinata outside of SWFL is not currently well understood. Targeted environmental DNA (eDNA) surveys were chosen to assess the occurrence of P …

Assessment Of Genetic Diversity In Largetooth Sawfish, Pristis Pristis, Populations Over The 20th Century, Ann Fearing

Master's Theses

The Critically Endangered Largetooth Sawfish, Pristis pristis, experienced global declines in range and abundance over the past century and Australia is now their last stronghold. This research aimed to understand whether these declines have been accompanied by a reduction in levels of genetic diversity. Using P. pristis tissue samples sourced from natural history specimens, three fragments (616-bp, 386-bp, 141-bp) in the mitochondrial DNA (mtDNA) control region were PCR-amplified. At each fragment, significant genetic structure was found in P. pristis overall (Φ_ST=0.946, N=9; 0.813, N=54; 0.771, N=99). The Indo-West Pacific and Atlantic/Eastern Pacific each harbor genetically distinct lineages of …

Effects Of Infection Of The Protist Parasite, Dermomycoides Sp., In Dusky Gopher Frog Tadpoles, Jaime Smith

Master's Theses

Infections of the protist parasite, Dermomycoides sp. are thought to have caused several years of low recruitment in the dusky gopher frog (Rana sevosa) populations. I evaluated the effects of density of the infective zoospores, host developmental stage, and tadpoles' ability to acquire resistance to Dermomycoides sp. on dusky gopher frog tadpoles. Tadpoles were exposed to zoospore densities of 0, 250, 500, and 750 zoospores/µL at Gosner stage 25, and we found no significant differences among treatments in tadpole mortality. In evaluating susceptibility by development stage, I exposed R. sevosa to 50 zoospores/µL as eggs, embryos, hatchlings, and …

Assessing The Nursery-Role Function Of Pelagic Sargassum For Juvenile Fishes In The Northern Gulf Of Mexico, Courtney Stachowiak

Master's Theses

Sargassum, a genus of holopelagic brown algae, floats at the ocean’s surface using air-filled bladders and forms a complex comprised of two species, S. natans and S. fluitans. Oceanic processes (e.g., Langmuir circulation, etc.) aggregate Sargassum into mats and weedlines, and primarily distribute the algal complex throughout the North Atlantic Ocean and the Gulf of Mexico (GOM). These floating habitats provide shelter and feeding opportunities for a diverse community of invertebrates and fishes. Sargassum is a presumed nursery habitat for juvenile stages of commercially- and recreationally-targeted fishery species. In this study, I estimated the standardized abundances of juvenile …

Determining The Trophic Role Of Red Snapper (Lutjanus Campechanus) In Mississippi State Waters Using Stomach Content And Stable Isotope Analysis, Branden Kohler

Master's Theses

The goal of this study was to determine the diet composition, trophic position and ecological role of red snapper (Lutjanus campechanus) in Mississippi state waters utilizing stable isotopes (δ¹³C and δ¹⁵N) and stomach content analysis. Stable isotope analysis of fish and their prey can provide information on species-specific basal resource utilization, diet composition and trophic position which can improve food web models and inform fisheries management decisions. Particulate organic matter (POM), the presumed base of the food web, red snapper muscle tissue, and red snapper stomach contents were collected from 25 sites in 2016 and …