Digital Commons Network^™

Isolation Of Antifungal Lactic Acid Bacteria From Food Sources And Their Use To Inhibit Mold Growth In Cheese, Dan Zhao

Master's Theses

A large amount of cheese is lost every year due to mold contamination. Biopreservation, which is the use of biological entities (microbes) and their metabolites to suppress microbial spoilage instead of chemical preservatives has lately gained increasing interest. Lactic acid bacteria (LAB) have the potential for use in biopreservation, because they are safe to consume and naturally exist in many foods. In this study, fifteen strains of lactobacilli isolated from dairy products, vegetables, and fermented pickles were tested by agar overlay assay for their anti-mold activity. Six strains grown on MRS agar showed strong inhibitory activity against a target mold …

The Effect Of Processing Parameters And Methylcellulose On Texture And Consumer Acceptability Of A Non-Melting Dairy Protein Gel, Joshua Goldman

Master's Theses

The overall goal was to understand the capabilities of a dairy based meat alternative.  This was done in three phases: 1) the production of a dairy protein gel as a base for a dairy based meat alternative, 2) texturization of the dairy protein gel base to produce a nugget like texture, and 3) a consumer test to determine the acceptability of the dairy based nugget compared to meat and meat alternatives on the market.

For phase I, a dairy protein gel base was tested to understand the textural attributes.  Milk to whey percentages and level of acidification were examined to …

Measuring The Nanoindentation Properties Of Alendronate-Time Treated Canine Cortical, Folorunso Ashaolu

Master's Theses

This study examines the nanoindentation (energy inclusive) properties of 0.2 mg/kg alendronate treated ribs at one and three years against a vehicle treated control in a fresh-frozen, non-cold-mounted, condition.  This was to verify if the tissue-level properties for 0.2 ALN treated beagles would increase because of an increased level of mineralization despite a microdamage increase.

A total of twelve (12) skeletally mature (1–2 years old) female beagle dogs were treated daily for three years and one year with oral doses of vehicle (VEH, 1 mL/kg saline) or alendronate (ALN, 0.2 Merck, Rahway, NJ). The 0.2 mg dose corresponds, on a …

Ischemia Impairs Vasodilation In Skeletal Muscle Resistance Artery, Kyle Remington Struthers

Master's Theses

Functional vasodilation in arterioles is impaired with chronic ischemia. We sought to examine the impact of chronic ischemia and age on skeletal muscle resistance artery function. To examine the impact of chronic ischemia, the femoral artery was resected from young (2-3mo) and adult (6-7mo) mice and the profunda femoris artery diameter was measured at rest and following gracilis muscle contraction 14 days later using intravital microscopy. Functional vasodilation was significantly impaired in ischemic mice (14.4±4.6% vs. 137.8±14.3%, p<0.0001 n=8) and non-ischemic adult mice (103.0±9.4% vs. 137.8±14.3%, p=0.05 n=10). In order to analyze the cellular mechanisms of the impairment, a protocol was developed to apply pharmacological agents to the experimental preparation while maintaining tissue homeostasis. Endothelial and smooth muscle dependent vasodilation were impaired with ischemia, 39.6 ± 13.6% vs. 80.5 ± 11.4% and 43.0 ± 11.7% vs. 85.1 ± 10.5%, respectively. From this data, it can be supported that smooth muscle dysfunction is the reason for the observed impairment in arterial vasodilation.

Factors Influencing Ectoparasitism On Western Fence Lizards (Sceloporus Occidentalis): Host Sex, Testosterone, Reproductive Condition, And Behavior, Nicholas B. Pollock

Master's Theses

Host-parasite relationships are one of the most common symbiotic relationships present in a diverse array of ecosystems. There are numerous factors that impact the dynamics of these relationships. Major factors that can influence the degree of parasitism include host sex, hormonal state, reproductive condition, and behavior. It has been observed in several vertebrate taxa that males have higher ectoparasite intensities than females and males with increased testosterone have increased ectoparasite intensities. One potential reason for these observations is that testosterone concentrations are elevated in males, particularly during the breeding season, and when circulating concentrations increase males become more vulnerable to …

A Frequency-Modulated Continuous Wave-Based Boundary Detection System For Determination Of Monitoring Region For An Indoor Ultra-Wideband Short Range Radar-Based Eldercare Monitoring System, Wilson Tang

Master's Theses

Falls are a cause of concern for the elderly since it can render a person immobile. A monitoring system can summon immediate aid by the automatic detection of fall events. The application of ultra-wideband (UWB) signals for a monitoring system was chosen due to its unique characteristics such as wide spectrum, immunity to interference, penetrability, and application for the detection of people. Part of this monitoring system is boundary detection with the purpose of isolating the location of fall events and to function as a mask to define an area of interest. A study of various localizing methods using measurements …

Point-Based Color Bleeding With Volumes, Christopher J. Gibson

Master's Theses

The interaction of light in our world is immensely complex, but with mod-

ern computers and advanced rendering algorithms, we are beginning to reach

the point where photo-realistic renders are truly difficult to separate from real

photographs. Achieving realistic or believable global illumination in scenes with

participating media is exponentially more expensive compared to our traditional

polygonal methods. Light interacts with the particles of a volume, creating com-

plex radiance patterns.

In this thesis, we introduce an extension to the commonly used point-based

color bleeding (PCB) technique, implementing volume scatter contributions. With

the addition of this PCB algorithm extension, we …

Go Farm, Goleta: Urban Agriculture Protection For Eastern Goleta Valley, Eli M. Krispi

Master's Theses

This paper explores two potential land use planning strategies that can be used to preserve and enhance the economic viability of agricultural operations surrounded by suburban development in Santa Barbara County’s Eastern Goleta Valley: buffers between agriculture and other land uses, and agritourism. In the case of buffers, academic literature is examined to determine how effective buffers are at various tasks (filtering runoff, mitigating dust and wind, providing habitat, etc.) and how to construct buffers to maximize their effectiveness. Land use plans and codes from several California jurisdictions are studied to see how buffers are put to use. Academic literature …

The Preparation Of Functional Surfaces, Philip Thomas Dirlam

Master's Theses

Diels-Alder chemistry was utilized to manipulate the surface energy of glass substrates in reversible manner. Glass slides and capillaries were functionalized with hydrophobic dieneophiles resulting in a  non-wetting surface.  A retro Diels-Alder reaction facilitated by the thermal treatment of the surface’s function to cleave the hydrophobic dieneophile and resulted in the fabrication of a hydrophilic surface. Contact angle (CA) measurements were used as preliminary measurements for monitoring the changes in surface energy exhibited during the initial hydrophobic state (CA - 70±3°), after attachment of the dieneophile creating  a hydrophobic state (CA - 101±9°) followed by reestablishment of the hydrophilic state …

Predicting Soil Erosion In The Santa Rosa Creek Watershed Using Rusle2 And Geographic Information Systems, Stacey Carol Smith

Master's Theses

The Santa Rosa Creek watershed is one of the most pristine watersheds on California’s Central Coast.  Preserving this watershed is of great interest because it provides rich soils for agriculture, vast rangelands for cattle, and flowing streams for federally threatened species such as steelhead trout.  Soil erosion could impact these resources.  Using prediction tools, it is possible to study the erosion that could be occurring in a watershed and identify locations which could contribute the highest amounts of sediment.  The objectives of this study were to use RUSLE2 and Geographic Information Systems (GIS) to predict soil erosion rates for each …

Magnetostatic Modeling For Microfluidic Device Design, Kirsten Marie Jackson

Master's Theses

Magnetostatic Modeling for Microfluidic Device Design

Kirsten Marie Jackson

For several years, biologists have used superparamagnetic beads to facilitate biological separations. More recently, researchers have adopted this approach in microfluidic devices [1-3]. This recent development and use of superparamagnetic particles in biomedical and biological applications have resulted in a necessity for methods that enable the understanding and prediction of their properties and actions during use. Typically, such methods would involve simple experimentation prior to in vitro experimentation, animal testing, and finally clinical testing. To better understand and unleash this technology, COMSOL®, which is a finite element analysis and multiphysics simulation …

Development And Extrapolation Of An Undergraduate Laboratory Experiment To An Elastomeric Spinal Muscular Atrophy Brace, Richard Sterling Brose

Master's Theses

Ever since the advent of polymer science, polyurethanes have played a huge role in the industrial world. They have been used in endless applications from furniture padding to aircraft coatings, to binders for insensitive munitions. It is therefore important that the chemistry of polyurethanes is well understood as well as the ability to draw relationships between the raw materials selected and the end-use properties of the polymer. Because of the multitude of practical applications, the development of an undergraduate polymer chemistry laboratory focused on polyurethane elastomers is developed and described herein. Polymer chemistry students are exposed to hydroxyterminated polybutadiene (HTPB) …

Developing Digital Field Guides For Plants: A Study From The Perspective Of Users, Emily Roseanne Schwarz

Master's Theses

A field guide is a tool to identify an object of natural history. Field guides
cover a wide range of topics from plants to fungi, birds to mammals, and shells to minerals. Traditionally, field guides are books, usually small enough to be carried outdoors . They enjoy wide popularity in modern life; almost every American home and library owns at least one field guide, and the same is also true for other areas of the world.

At this time, companies, non-profits, and universities are developing computer
technologies to replace printed field guides for identifying plants. This thesis
examines the state …

A Neural Network Receiver For Em-Mwd Communication, Timothy P. Whitacre

Master's Theses

Baseband digital communication in electro-magnetic measurement while drilling (EM-MWD) systems is often corrupted by non-white surface noise. The inability to reliably decode the transmitted signals in a noisy environment limits the depth at which EM-MWD systems can operate. Correlation receivers, which are optimal in the presence of additive white Gaussian noise, can be sub-optimal in the presence of various types of field noise at different drilling sites.

This thesis investigates the application of artificial neural networks (ANN) as communication receivers in EM-MWD baseband digital communication systems. The performances of various ANN architectures and training algorithms are studied and compared with …

Active Pen Input And The Android Input Framework, Andrew S. Hughes

Master's Theses

User input has taken many forms since the conception of computers. In the past ten years, Tablet PCs have provided a natural writing experience for users with the advent of active pen input. Unfortunately, pen based input has yet to be adopted as an input method by any modern mobile operating system. This thesis investigates the addition of active pen based input to the Android mobile operating system.

The Android input framework was evaluated and modified to allow for active pen input events. Since active pens allow for their position to be detected without making contact with the screen, an …

Optimizing Micronutrient Intake Of Lactating Women In Kwazulu-Natal, South Africa, Through Increased Wild Edible Plant Consumption, Kerry Pearson

Master's Theses

OPTIMIZING MICRONUTRIENT INTAKE OF LACTATING WOMEN IN KWAZULU-NATAL, SOUTH AFRICA, THROUGH INCREASED WILD EDIBLE PLANT CONSUMPTION

Kerry Pearson

Micronutrient consumption in KwaZulu-Natal, South Africa, is insufficient to meet the needs of lactating women.  Inadequate intake negatively impacts the health of both the mother and infant.  Increasing consumption of wild edible plants has been recommended to combat these deficiencies, but information has not yet been provided on which types of wild plants should be eaten or in what quantity.  The objective of this study was to determine the optimal mixture of wild edible plants that needs to be consumed to meet …

Assessment And Improvement Of Fire Resiliency For Structures Located In The Wildland-Urban Interface, Allen L. Meskimen

Master's Theses

The purpose of this research was first to study the Wildland-Urban Interface and Wildland-Urban Intermix (WUI) fire problem, and then to design, develop and implement improved fire assessment and fire protection features for structures in the these interface fire-prone areas. The findings included that several areas of the world are prone to devastating fires that claim lives and destroy property, and their fire problems continue to exacerbate. None of these compare to the property loss experienced in Southern California due to its vast development in fire prone areas. It is because of the continuing huge property loss and frequency of …

Irish Hills Natural Reserve Conservation Plan Update, Bijan N. Riley

Master's Theses

The Irish Hills Natural Reserve Conservation Plan is a unique component of the City
of San Luis Obispo’s green belt that provides a number of hiking and mountain biking
trails within a short distance from the downtown core as well as ensures that valuable
habitat be preserved into the future. As the subject of my thesis project, IHNR presented the opportunity to pursue an aspect of city planning that can often be over-looked and, conservation planning. Conservation planning is an important element of the planning profession in that it incorporates such elements into the future growth of cities. With proper …

Short-Distance Translocation Of The Northern Pacific Rattlesnake (Crotalus O. Oreganus): Effects On Volume And Neurogenesis In The Cortical Forebrain, Steroid Hormone Concentrations, And Behaviors, Matthew L. Holding

Master's Theses

The hippocampus of birds and mammals has been shown to play a crucial role in spatial memory and navigation. The hippocampus exhibits plasticity in adulthood in response to diverse environmental factors associated with spatial demands placed on an animal. The cortical telencephalon of squamate reptiles has been implicated as a functional homologue to the hippocampus. This study sought to experimentally manipulate the navigational demands placed on free-ranging northern Pacific rattlesnakes (Crotalus o. oreganus) to provide direct evidence of the relationship between spatial demands and neuroplasticity in the cortical telencephalon of the squamate brain. Adult male rattlesnakes were radio-tracked …

Impact Of Exercise On Brain Responses To Visual Food Cues: An Fmri Study, Nero Erezi Evero

Master's Theses

On the basis of a strong body of data, the Institute of Medicine currently recommends at least 60 minutes of exercise per day to prevent body weight gain overtime.  Previous studies have shown that there is no compensatory increase in food intake with this dose of exercise. Ultimately, the brain decides whether to alter food intake.  Surprisingly, no published studies have assessed the impact of exercise on brain activation.  Using functional magnetic resonance imaging (fMRI) and an appetite questionnaire, we investigated the effects of a single bout of aerobic exercise on brain responses to visual food cues and subjective appetite …

Studying Milk Coagulation Kinetics With Laser Scanning Confocal Microscopy, Image Processing, And Computational Modeling, Richard Joseph Hennessy

Master's Theses

The kinetics of milk coagulation are complex and still not well understood. A deeper understanding of coagulation and the impact of the relevant factors would aid in both cheese manufacturing and also in determining the nutritional benefits of dairy products. A method using confocal microscopy was developed to follow the movement of milk fat globules and the formation of a milk protein network during the enzyme-induced coagulation of milk. Image processing methods were then used to quantify the rate of coagulation. It was found that the texture of the protein network is an indicator of the current status of the …

Characterization And Implementation Of A Decellularized Porcine Vessel As A Biologic Scaffold For A Blood Vessel Mimic, Aubrey N. Smith

Master's Theses

Every 34 seconds, someone in the United States suffers from a heart attack.  Most heart attacks are caused by atherosclerotic build up in the coronary arteries, occluding normal blood flow.  Balloon angioplasty procedures in combination with a metal stent often result in successful restoration of normal blood flow.  However, bare metal stents often lead to restenosis and other complications.  To compensate for this problem, industry has created drug-eluting stents to promote healing of the artery wall post stenting.  These stents are continually advancing toward better drug-eluting designs and methods, resulting in a need for fast and reliable pre-clinical testing modalities. …

The Ma-10 Cell Line As A Model Of Insl3 Regulation And Leydig Cell Function, Mary E. Strong

Master's Theses

Leydig cells produce testosterone in response to luteinizing hormone (LH) via the cyclic adenosine monophosphate (cAMP)/protein kinase A pathway. Additionally, these cells are responsible for producing insulin-like peptide 3 (INSL3), a peptide hormone that is essential for testicular descent. The insl3 promoter in Leydig cells can be activated by cAMP through the transcription factor Nur77, which has also been shown to regulate the promoters of the steroidogenic enzymes, cyp17 and 3b-hsd. While the mechanism of LH action on testosterone production is well characterized, the effect of LH on insl3 abundance has yet to be shown directly. The MA-10 …

An Analysis Of Technology And Management Gaps For Groundnut Producers In Kenya, Angelista Kihaga Ms

Master's Theses

No abstract provided.

Optimization Of Culture Conditions For Equine Bone Marrow Mesenchymal Stem Cells And Their Differentiation Into Osteoblasts, Elizabeth R. Ackell

Master's Theses

The horse industry is a multibillion dollar industry where the health of the animals is of great focus for horse owners.  Bone fractures are an aspect of equine health failure that is continually catastrophic and often fatal.  In addition to discussing fracture, this review will outline the process by which bone tissue is regularly maintained and self-healed.  Also, traditional methods of healing fractures that have limited use and success will be discussed.  The lack of success that is common with the traditional methods has led to the need for additional research to identify more successful and economical ways of healing …

The Effect Of Total Work Performed During Acute Heavy Resistance Exercise On Circulating Lymphocytes In Untrained Men, Neil A. Kelly Jr

Master's Theses

Lymphocytes are one of many leukocytes which exert a biphasic response to acute intense resistance exercise. Exercise volume (i.e. total work) has been shown to have an immunomodulatory effect. This study evaluates the effect of total work performed during an intense resistance exercise protocol on lymphocyte concentrations in the circulation. Untrained, college-aged (18-35) males who performed high amounts of work (HW) were compared to those who performed low amounts of work (LW). Resistance exercise testing consisted of 6 sets of 10 repetitions of the squat exercise with 2 minutes rest between sets. Both HW and LW performed the same relative …

Deficits In Auditory, Cognitive, And Motor Processing Following Reversible Mcao In Mice: Understanding The Human Stroke Phenotype, Dongnhu Truong

Master's Theses

Stoke is characterized by a loss or alteration in neurological and/or bodily function resulting from a cerebral vascular accident (interruption of blood flow to the brain).  Acute ischemic stroke is the third leading cause of death in the United States, and the leading cause of long-term functional disability in adults.  Stroke-induced deficits may include various forms of aphasia (language loss), cognitive deficits (including attentional and memory impairments), and motor impairments.  Through the use of animal models, researchers can experimentally induce “stroke-like” injuries comparable to those seen in clinical populations.  Such models allow us to study and understand the neurophysiological, anatomical, …

Distance Judgments For Joint Action: The Perceptual Consequences Of Anticipated Coordination, Benjamin R. Meagher

Master's Theses

Recent perception research has revealed that judgments of distance are influenced by the energetic cost required to perform particular actions, such as walking, across these distances (Proffitt, 2006b). However, this prior research has focused almost exclusively on the perceptual consequences of solo action, despite the fact that individuals regularly become embedded within social units for the purpose of joint action (Richardson, Marsh, & Schmidt, 2005). In two experiments, the current work sought to test the hypothesis that forming a social unit creates a new perception-action system with distinct perceptual attunement of the environment scaled to the unit’s action-potential. Participants, accompanied …

Animation And Visualization Of 3d Underwater Sensor Networks, Matthew T. Tran

Master's Theses

Simulation and visualization are critical for the development of new systems and protocols in the area of computer networking. As real-world field testing is expensive and time-consuming, simulations are often preferred as they can be performed repeatedly and inexpensively while still reflecting the outcome of field tests to an extent. Visualizations of the simulation and field test results often follow to provide researchers with a vivid animation of the events, allowing for a much more intuitive understanding of the system than tediously reading through trace files. While there are currently a multitude of simulators and animators for land-based networks, few …

Comparison Of An Ultrasonic And A Non-Ultrasonic Endodontic Irrigation Protocol: A Clinical Study, Christopher M. Beus

Master's Theses

The use of irrigation as a method of rendering canals bacteria free continues to be a source of investigation. Recent data suggests that an activated irrigation protocol such as passive ultrasonic irrigation can result in decreased intracanal bacteria. In addition, studies have shown that the activation of various irrigants such as NaOCl and Chlorhexidine can increase the sum antimicrobial effect. The purpose of this randomized, double blinded, prospective in-vivo clinical study was to compare the results of a non-activated irrigation protocol (NAI) using only NaOCl 1.5% to a passive ultrasonic irrigation protocol (PUI) using NaOCl 1.5%, EDTA 17% and Chlorhexidine …