No One Really Knows You, Andrew Charles Rhodes

Master's Theses

This is a collection of five short stories that I have written during my time in the Center for Writers at the University of Southern Mississippi. The stories were written, revised, and workshopped in graduate classes over the last two years.

Ph-Responsive Polymers For Biosurfaces: An Investigation Of Solution Properties And Surface Modifying Performance, Yuhong Wei

Master's Theses

Gantrez® copolymers are used widely in the personal care industry, but their mechanisms of interaction with dental surfaces are poorly understood. In this study, we determined a number of the solution and polymer properties that control deposition, adsorption and adhesion in Gantrez® polymer aqueous solutions to model dental surfaces. QCM studies specifically showed that polymer adsorption to model hydroxyapatite surfaces occurred only in solutions with polymer concentration above c*. Adsorption and adhesion was greater in solutions of higher pH, where greater charge density along the polymer backbone was expected. For films prepared via spin-coating onto mica surfaces, addition of surfactants …

Investigation Of A Commercial Product (Biowish™) For Nitrogen Management, Eva Lee

Master's Theses

Abstract

Investigation of a Commercial Product (BiOWiSH­­^TM) for Nitrogen Management

Eva Lee

BiOWiSH–Aqua, which has the capability of treating nitrogen from wastewater through bioaugmentation, is a commercial product consisting of a blend of microorganisms developed by BiOWiSH Technologies. A study of the treatment of nitrogen compounds (i.e. , , and ) using Biowish–Aqua was conducted using small scale experiments (flask experiments) and large scale experiments (column reactor experiments). In this work, column reactors were created to test Biowish–Aqua’s nitrogen treating capabilities by providing enough depth to simulate the dissolved oxygen gradient that can be observed in a pond. …

American Pika (Ochotona Princeps): Persistence And Activity Patterns In A Changing Climate, Cody P. Massing

Master's Theses

An increasing amount of evidence suggests that as temperatures increase, montane animals are moving upward in elevation (IPCC 2007, Parmesan and Yohe 2003). As suitable habitats rise in elevation and then disappear altogether, these animals could be pushed to extinction. The American pika, Ochotona princeps, is a montane mammal that lives in western North America, usually at elevations above 1500 m (Smith and Weston 1990). Recent evidence suggests that pika population numbers are dropping in response to rising temperatures (Beever et al. 2010). The pika is a small herbivorous lagomorph, a relative of hares and rabbits. Its habitat is …

Autonomous Tracking And Following Of Sharks With An Autonomous Underwater Vehicle, Esfandiar Manii

Master's Theses

This thesis presents the integration of an acoustic tracking system within an autonomous underwater AUV (AUV) to enable real-time tracking of sharks tagged with artificial acoustic sources. The tracking system consists of two hydrophones and a receiver unit that outputs a measurement of the relative angle to the tagged shark. Since only two hydrophones are used, the sign of the relative angle measurement is unknown. To overcome this ambiguity, a particle filter algorithm was developed to estimate the position of the acoustic source. When combined with an active control system that drives vehicle to obtain different orientations with respect to …

Best Management Practices Effectiveness To Reduce Sediment Transport To Morro Bay, Michael J. Randall

Master's Theses

The Morro Bay Watershed, which is located inSan Luis Obispo County,California, covers more than 48,000 acres of land and discharges intoMorroBaythrough the Morro Bay National Estuary (MBNE). The Chorro Creek Subwatershed consists of approximately 30,000 acres of the overall watershed. The MBNE provides an ecosystem that supports a variety of wildlife from the common sea gull to the endangered sea otter. The estuary is also home to over 200 species of birds. The operational waterfront of theMorroBayHarborwas and continues to be a strong supporter to the local economy of the City of Morro Bay. Numerous studies were conducted since the …

Mechanical Properties Of Bone Due To Sost Expression: A 3-Point Bending Assessment Of Murine Femurs, Kainoa John Peterson

Master's Theses

Sclerostin, a protein coded for by the SOST gene, is an osteocyte-expressed negative regulator of bone formation. The absence of SOST in the genome may have an effect on bone formation both during skeletal maturation and full maturity. This study attempts to determine significant differences in the mechanical properties of bone that expresses SOST compared to bone that does not. One hundred femur samples from 6, 8, and 12 month old mice were obtained from Lawrence Livermore National Labs and loaded until failure using three-point bending. Results showed significant differences in treatment group effects for cross sectional area, yield force, …

Detection Of Sk2 Channels On Hippocampal Neurons, Jamie L. Maciaszek

Master's Theses

Calcium-activated small conductance potassium channels (SK) are crucial for synaptic plasticity, sleep, and learning and memory (Hammond, Bond et al. 2006; Cueni, Canepari et al. 2008; Lin, Lujan et al. 2008). Despite the recent progress on SK channel physiology, the precise spatial organization of SK channels in neurons has remained unknown. Such knowledge is critical as the subcellular distribution of SK channels is an important determinant of neuronal excitability. Currently, there are no techniques to image ion channel distribution quantitatively at the nanometer scale in living cells. Here, it is demonstrated that integration of natural toxins with single molecule atomic …

The Impact Of Increased Collateral Flow On Transverse Arteriolar Microvascular Tree Morphology, David Danzeiser

Master's Theses

The goal of this study was to understand how arteriogenesis impacts transverse arteriolar tree structure. The previous process involved a vascular casting technique that utilized black India ink as the casting agent and manual measurement of vessel morphology with ImageJ. This study decreased the complexity of the casted samples by using a casting compound called Microfil. Microfil’s viscosity can be adjusted and through experimentation, a viscosity was found that stopped at the capillary level, allowing only the arterial side of the circulation to be casted. A macro in ImageJ was written to help with morphological measurement by bundling vessel length …

Combustion Simulations Using Graphic Processing Units, Mingjie Wang

Master's Theses

Graphic processing units (GPUs) are powerful graphics engines featuring high levels of parallelism and extreme memory bandwidth, which constitute a powerful computing platform to solve complex problems involving chemically reacting flows. In the present study, computer programs for combustion simulations with detailed chemical kinetic mechanisms were compiled in the Compute Unified Device Architecture (CUDA) language for NVIDIA GPU architecture. Ignition processes were simulated under constant pressure and constant volume conditions using an explicit 4^th order Runge-Kutta algorithm for time integration. Sufficiently small time steps were identified with time scale analysis to ensure the integration stability. The program was validated …

The Proteomic Response Of Sea Squirts (Genus Ciona Congeners) To Hyposalinity Stress, James S. Koman

Master's Theses

The ascidian species Ciona savignyi and C. intestinalis are invasive species but show interspecific differences in their population response to hypo-saline stress associated with heavy winter-run off events that are predicted to become more frequent due to climate change. Despite an almost world-wide distribution, C. intestinalis seems to be more susceptible to hypo-saline stress than the geographically more limited C. savignyi. Given that the genomes of both species are fully sequenced, we were able to compare their proteomic response to both acute and chronic salinity to characterize the mechanisms that are responsible for setting tolerance limits to hyposaline conditions …

Vitamin D Sub-Set Analysis From The Flash Study, Megan Bishop, Laura Hall, Ann Mcdermott, Aydin Nazmi

Master's Theses

Vitamin D is important to the health of college students. The objective of our study was to measure sun exposure, skin pigmentation, vitamin D intake, and serum 25-hydroxyvitamin D (25[OH]D) in a subset of participants from The Following the Longitudinal Aspects of Student Health (FLASH) Study to determine the best predictors of 25(OH)D status. Participants were college-aged freshman who had their blood drawn in spring (Visit 1) and fall 2010 (Visit 2) at California Polytechnic State University (Cal Poly), San Luis Obispo, CA. (35.3°N). Vitamin D intake was measured using a 28-day food frequency recall questionnaire (specific to vitamin D …

Development Of A Standardized Method For Comparing Biomechanical Properties Of Various Sternal Closure Techniques, Ramzi P. Hawit

Master's Theses

Background:

33.6% of all deaths in America are caused by cardiovascular disease. An estimated 82.6 million adults (>1 in 3) in America have some form of cardiovascular disease. There were over 400,000 bypass surgeries requiring open-heart surgery. Sternal dehiscence is associated with a morbidity rate of over 47% if mediastinitis supervenes. A rigid closure is required to avoid healing complications, and wire, plates, and bands are all used in an attempt to make a better closure. The purpose of this study it to compare multiple closures and validate a new testing method.

Methods:

Polyurethane foam blocks will be used, …

Bacterial Source Tracking Of E. Coli In A Constructed Wetland, Rose M. Martin

Master's Theses

Bacterial source tracking was used to identify sources of fecal contamination in a constructed wetland. Nucleotide sequence differences in the Escherichia coli malate dehydrogenase (mdh) gene were used to distinguish between strains isolated from pigeon and cattle feces. Fourteen E. coli isolates were taken from cattle and pigeon fecal samples and sixteen E. coli isolates were taken from wetland water samples. A region of the E. coli mdh gene was amplified via PCR and sequenced. Twelve distinct sequences were obtained. Water samples indicated the presence of both pigeon and cattle fecal contamination in the wetland. Six sequences distinct …

The Multi-Scale Dynamics Of Executive Function, Jason Anastas

Master's Theses

Cognitive control is a central issue in developmental psychology. Traditional theories of psychology solve this problem by positing a top-down central executive, which coordinates cognitive resources in pursuit of goals. We propose an alternative explanation: cognitive control arises from physical interactions across many different timescales within the system. College and preschool aged participants were asked to complete a simple executive function task, card sorting. We found that multi-scale physical interactions differed depending on experimental constraints, and that executive function in these cases was driven primarily by flexibility in multi-scale interactions, rather than the dominance of one scale. This suggests that, …

Desert Hedgehog Is A Mammal-Specific Gene Expressed During Testicular And Ovarian Development In A Marsupial, William A. O'Hara

Master's Theses

Desert hedgehog (DHH) belongs to the hedgehog gene family that act as secreted intercellular signal transducers. DHH is an essential morphogen for normal testicular development and function in both mice and humans but is not present in the avian lineage. Like other hedgehog proteins, DHH signals through the patched (PTCH) receptors 1 and 2. Here we examine the expression and protein distribution of DHH, PTCH1 and PTCH2 in the developing testes of a marsupial mammal (the tammar wallaby) to determine whether DHH signalling is a conserved factor in gonadal development in all therian mammals.

The Hybrid Laboratory: Informal Spaces For Public-Science Interaction, Whitney Ann Ferrin-Rodriguez

Master's Theses

While growing trends in participatory science and public outreach have made science more accessible to publics, science communities and publics have long interpreted science and laboratories as private domains, perpetuating the idea that science is reserved for "experts" and locations for science are deemed "private." Many studies that examine public-science interaction, however, seldom consider the role of space and place. I argue that material spaces shape public-science interactions. My analysis takes place at an institutionalized informal education platform: a hybrid laboratory: a space used by "expert" astronomers to create new visual knowledge and "lay" publics to actively participate in astronomy. …

Determination Of The Molecular Mechanisms Of Hyperglycemia-Induced Changes In Cav3.2 Calcium Channel Properties, Ginny Marie Keeling

Master's Theses

Hyperglycemia can cause altered excitability due to increased CaV3.2 T-type calcium channel function, bestowing diabetics an increased neuropathy risk. The objective of this study was to understand the molecular mechanisms of increased CaV3.2 function during hyperglycemia in vitro, which leads to a 58% increase in current density and increased channel open probability (PO), among other changes. Two major findings are reported in this study:

1) The enzymatic elimination of extracellular N-acetylneuraminic acid (NANA) moieties reversed the increase in current density and the shift in PO, showing that hyper-glycosylation alters channel function in disease.

2) Treatment with GM1 (a ganglioside highly …

Urinary Antimicrobial Peptides And The Urinary Microbiota In A Uti-Susceptible Population Of Female Pelvic Floor Surgery Patients, Vanessa Nienhouse

Master's Theses

Urinary tract infections (UTI)s are a national priority. Women who undergo surgery for pelvic floor disorders such as pelvic organ prolapse (POP) or urinary incontinence (UI) are at increased risk for UTI, as 10-30% will contract a post-instrumentation UTI (postI-UTI) within six weeks after surgery. Currently, the factors that contribute to the high rate of postI-UTI are unknown, and there is currently no clinical assessment to identify at-risk patients. However, antimicrobial peptides (AMPs) and characteristics of the urinary microbiota have the potential to serve as biomarkers, identifying patients at UTI risk and facilitating clinical prevention studies.

While it was previously …

Binge Ethanol Leads To Decreased Macrophage Accumulation In Infected Cutaneous Wounds, Sara Hlavin

Master's Theses

Trauma patients who consumed alcohol prior to sustaining injuries have higher rates of morbidity and mortality than those with comparable injuries who did not drink. Additionally, those who drank had impaired wound healing and increased susceptibility to infection. Despite these clinical observations, few studies have explored the effect of ethanol on the innate immune cell function in a healing wound or how this may alter resolution of cutaneous infection. A murine model of ethanol and cutaneous wound infection was used to examine bacterial growth and recruitment of innate immune cells. Mice were given either ethanol (2.2 g/kg) or saline 30 …

Climate-Related Floral And Vegetative Size Variation In Leptosiphon Androsaceus (Polemoniaceae), Aggie Morrow

Master's Theses

Phenotypic plasticity in plants is a vital adaptive response to changing environmental conditions. Floral and vegetative morphology often varies as biotic and abiotic factors vary. Variation is the basis of evolution, and natural selection, acting on trait variation, can lead to speciation. For this study, floral and vegetative character traits of false babystars (Leptosiphon androsaceus, Polemoniaceae) were analyzed within four populations at Henry W. Coe State Park in 2011. Traits were measured to assess changes in size along a moisture availability gradient. Stable carbon isotope ratio samples were collected to measure integrated water-use efficiency (WUE) as it related to precipitation …

The Biology And Ecology Of Six Rare Plants From Plumas National Forest, Northern California, Usa, Suzie Woolhouse

Master's Theses

The majority of our knowledge about the natural world has come from the study and observation of common species, yet a significant portion of species in the world are rare. In this study, biological and ecological data was collected for six rare taxa from Plumas National Forest. The taxa were Monardella stebbinsii, Monardella follettii (Lamiaceae), Lewisia cantelovii (Montiaceae), Cypripedium californicum, Cypripedium fasciculatum (Orchidaceae) and Clarkia mildrediae subsp. mildrediae (Onagraceae). The two Monardella's and Cypripedium californicum are serpentine endemics, and the Lewisia is found growing both on and off serpentine. Five sites were visited per species, and soil samples …

Microsatellite And Mitochondrial Dna Analysis Of Dungeness Crab (Cancer Magister) From California To Northern British Columbia, Bryan Thomas Barney

Master's Theses

Genetic variation was assessed using mitochondrial DNA and microsatellite markers from Dungeness crab between Iceberg Bay, British Columbia and San Luis Obispo, California. We found little pattern in overall genetic variation between sites in both marker types, and no significant Isolation by Distance model was fit. Site-specific variation in mitochondrial DNA haplotype frequencies suggested the existence of three subpopulations associated with the Alaska Current, the Puget Sound, and the California Current, but microsatellite DNA evidence did not support it. The ratio between sampling size for microsatellite markers and fragment size polymorphisms was low, limiting the resolving power of microsatellite DNA …

The Gold Mountain Theater Riots: A Social History Of Chinese Theater Riots In San Francisco During The 1870s And 1880s, Morgan Gerard Boyd

Master's Theses

During the 1870s and 1880s, San Francisco's Chinese theaters were scenes of tumultuous riots, which this thesis has designated as The Gold Mountain Theater Riots. City petitions and ordinances restricting the performances of Chinese theaters, police raids on Chinese theaters when they did not comply with these ordinances, stampedes caused by panics, and tong and Chinese theater rivalries were all catalysts for violence in America's first Chinese theaters. The Gold Mountain Theater Riots attempts to gain further knowledge of why these extra-theatrical events occurred, through the examination of Chinese audiences and the police involved in these theater riots as reported …

Rhetoric Of Social Change In Documentary Film Scores: An Analysis Of The Cove, Brianna Chanel Hackley

Master's Theses

Documentary films tell the untold stories and tragedies of the world as well as reflect the filmmakers' ideologies. In this way, they are socially conscious media: they give light to the struggles and problems that exist in society, often giving voice to people who might otherwise be silenced. Documentary films are also rhetorically persuasive in that they help to organize public perception. Documentary scores are part of films' persuasiveness and help audiences to make sense of the documentary footage.

Using a form of rhetorical analysis, this thesis aims to study documentary scores to understand how they mobilize audiences around a …

Producing Short And Long Run Projections For The Ecological Footprint, David Gordon Moore

Master's Theses

The Ecological Footprint is a useful tool for public awareness of ecological pressures and for policymakers who aim to reduce them. In order to determine the potential effects of future actions and policies, it is necessary to construct scenarios of future global conditions, both in the short-term and long-term. This study develops two alternative methods for creating Ecological Footprint scenarios: first using asymmetric changes in simple economic output (GDP) to look at short-term projections; then using widely accepted scenarios from international agencies to develop long-term projections.

Changes in GDP were found to be causal in determining changes in the Ecological …

A Morphological Study Of Eriogonum Nortonii (Polygonaceae) And Implications For Its Biogeography, Lisa Deree Morton

Master's Theses

Morphological characters, phenology, and abiotic parameters were measured to determine whether divergence has occurred between populations of Eriogonum nortonii E. Greene on the east and west sides of the Salinas Valley. Morphological variability was demonstrated across all populations but not between populations on opposite sides of the valley. Investigations into present and paleoenvironmental factors as well as dispersal factors suggest recent episodes of long distance dispersal were responsible for the disjunct distribution of E. nortonii populations.

More Myself, Karin Louise Mckie

Master's Theses

More Myself by Karin McKie is about living in the margins of the social network. As an ad hoc anthropologist, I survey my journeys, both internal and external, with a wry eye. I have lived alone since 1993, work mostly from home, no texting on my phone, and no kids ... that I know about. Throughout these essays, I chronicle the differences, as well as the intersections, between loneliness and being alone.

What is the nature of solitude? Am I worth knowing; am I worth loving? How did I get where I've got? From Virginia to Chicago and San José, …

Health Implications Of Mercury, Selenium, And A Red Pelage In Pacific Harbor Seals (Phoca Vitulina Richardii) Off Central California, Elizabeth Alexis Mchuron

Master's Theses

San Francisco Bay (SFB) is the largest estuary on the west coast of the United States. It provides habitat for many species, although is heavily urbanized with a history of selenium (Se) and mercury (Hg) contamination. Harbor seals (Phoca vitulina) are good indicators of the health of SFB because they are long–lived, upper–level trophic consumers, and present in the estuary year–round. The objective of this study was to examine the role of Se and Hg contamination on the health of harbor seals in this region, and the role of Se in the development of a red pelage. Between 2009 and …

Cracking The Coven: Shakespeare, The Supernatural, And The Female Power Base, Doll (Heather) Elizabeth Piccotto

Master's Theses

There has been extensive writing and research into the fairy magic and witchcraft practices of the Early Modern Period in the 400-plus years between when Shakespeare's plays were performed and now--even including a tome on demonology by King James I himself. However, as witchcraft and fairy magic are distinctively female realms, with women making up 90% of accused witches and fairy magic being mainly related to domestic duties, one cannot accurately discuss these phenomena in the plays without addressing how they affect the female characters. This project examines the role of the supernatural in three of Shakespeare's plays, A Midsummer …