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The Proteomic Response Of The Carcinus Maenas Y-Organ Over The Course Of The Molt Cycle, Mark S. Hamer

Master's Theses

Molting in arthropods is a complex process governed by regulatory mechanisms that have evolved and adapted over millennia to allow these animals to grow, despite being confined by a hardened exoskeleton. We isolated the molt-regulating Y-organs (YO) from the common shore crab Carcinus maenas at molt stages B, C_1-3, C₄, and D₀ to assess how changes in protein abundances might underline the unique physiology of each of these stages. We found that changes in protein abundance were most notable in the postmolt stages (B and C_1-3), where an increase in energy metabolism and …

Modeling The Effects Of Vertically Variable Soil Parameters And Hillslope Shape On Runoff, Benjamin L. Smith

Master's Theses

Soil parameters used in hydrologic modeling, such as porosity, volumetric water content, and saturated hydraulic conductivity (K_sat) are commonly known to be variable over depth within the soil profile, however these parameters are often modeled as single values, constant over depth. Also, hillslopes found in glacial till features of Connecticut tend to have an S-shaped profile over distance. This study proposes that using variably vertical soil parameters and hillslope shape has effects on runoff modeling results. The effects of vertical soil heterogeneity and hillslope shape on runoff production are evaluated by comparing soil water drainage and runoff …

Embedded Power Active Contact Lens, Benny Ng

Master's Theses

This thesis designed and fabricated an active contact lens that notifies the user during the detection of an external wireless signal. The lens contained a printed antenna to communicate with a 2.4GHz system and provide inductive charging operating at 13.56 MHz. The lens utilizes a CBC005 5µAh thin film battery by Cymbet as a power source. A custom IC was designed using the On Semiconductor CMOS C5 0.6 µm process to manage the battery and drive the display. A printed single element display using electrochromic ink was chosen as it is able to indicate the user when activated while staying …

Experimental Evolution Of Specialism In A Wild Virus, Alexander Kula

Master's Theses

A pathogen's ability to successfully replicate and persist within a new host population is fraught with obstacles. While an expanded host-range allows for a greater chance at successful replication, such generalists are typically outcompeted by species which have specialized and adapted host-specific features. Although the most ubiquitous species on earth, very few bacteria-infecting viral species (bacteriophages) with truly broad host-ranges have been identified; this is partially due to the fact that only a small fraction of bacteria (and thus likely hosts) are amenable to laboratory conditions. Nevertheless, the processes of expanding as well as narrowing host-range are not well understood …

Expression And Characterization Of Ecm14, A Metallocarboxypeptidase From Saccharomyces Cerevisiae, Matthew James Schott

Master's Theses

Metallocarboxypeptidases are found in most organisms and function in the digestion and maturation of proteins. Ecm14 is a putative metallocarboxypeptidase found in the yeast Saccharomyces cerevisiae vacuole. The function of Ecm14 as an enzyme has been unclear due to the presence of active site amino acids not typically found in metallocarboxypeptidases, suggesting either no enzymatic activity or a unique mechanism. In order to investigate the enzymatic mechanism of Ecm14, expression of histidine-tagged Ecm14 protein was attempted in human HEK293T cell culture, S. cerevisiae, and baculovirus expression systems. No expression was detected in HEK293T cells in preliminary experiments. Expression in the …

Shifting The Stress Curve: Using "Stress Inoculation" And Exercise To Promote Resilience, Mikyung Kim

Master's Theses

Problem

Stress influences an organism’s physiological systems via an inverted u-shaped curve: An optimum amount of stress will optimize body functions, but too little stress or too much stress for long periods of time can impair body functions. Researchers have been very interested in exploring the mechanisms that may “delay the tipping point” between the positive and negative effects of stress. A rightward shift in the stress curve would allow one to maintain optimal performance even at higher or more prolonged stress levels. The molecular and cellular mechanisms that underlie this rightward shift could result in resilience, clinically defined as …

Selective Processing By The L3 Auditory Interneuron In Acheta Domesticus : Juvenile Hormone Iii And Protein Kinase C, Ashley Ranae Lynch

Master's Theses

Juvenile Hormone III (JHIII) is a prolific and essential hormone in insects, controlling many aspects of insect physiology such as egg development, nymphal and larval maturation, diapause and metamorphosis. In female crickets, Acheta domesticus, JHIII increases selectivity in phonotactic behavior, narrowing phonotactic choices for Syllable Periods (SPs) of the Calling Songs (CSs) that most closely resemble the natural call of the male. JHIII has been suggested to work through a pathway that activates the protein kinase C (PKC) molecule. Chelerythrine Chloride (CC) is a potent inhibitor of PKC action. This study analyzes the responses of the L3 auditory interneuron, which …

Ranking Interesting Changes In Correlation Coefficient Matrix Results From Varying Data Partitions In Causal Graphic Modeling, Yesica Daniela Bravo Gonzalez

Master's Theses

Problem

In life we need to compare situations in order to select the best solution. The study in this paper is about analyzing data (variables), which is also called data mining. There are situations where it is not enough to compare variables among themselves at one specific moment. Sometimes it is necessary to compare the behavior of variables at different periods of time and know how they behave at different times in order to select the best arrangements for any situation.

Method

To find correlation among variables, traffic intersections were simulated so they could be compared, since the correlation coefficient …

Figurines From Tell Balatah/Shechem, Jacob A. Moody

Master's Theses

Problem

The Drew-­McCormick Expedition conducted six seasons of excavation at the site of Tell Balâtah/Shechem between 1957-­‐1968. During those campaigns they uncovered several anthropomorphic and zoomorphic (clay and stone) figurines. This study represents the initial research for those figurines and their eventual publication and will briefly review the pertinent research done on figurines from the Levant and provide a detailed catalogue of the available figurines from Tell Balâtah/Shechem.

Method

The 38 available figurines were studied and detailed drawings were provided for each. The known data associated with every figurine are specified (provenance, registration number, size, condition, physical description, etc.) and, …

Effects Of Acute Exercise, Mindfulness Meditation, And Mindfulness Meditation Neurofeedback On Stroop Performance, Claire Evelyn Balgemann

Master's Theses

Empirical research has demonstrated that acute aerobic physical exercise, neurofeedback, and mindfulness meditation independently have positive effects on executive performance. Technology has enabled a new intervention that combines neurofeedback and mindfulness meditation; however its effects on executive function are unknown. The aim in the present study was to understand how executive function may be enhanced in healthy individuals. Forty-two participants were randomly assigned to one of three intervention groups: acute physical exercise, mindfulness meditation, or mindfulness meditation neurofeedback delivered via the Muse brain-sensing headband. Participants completed 50 trials of the Stroop task, a well-established measure of executive function. A statistically …

Though My Gross Blood Be Stain'd: Bleeding Bodies And Power Dynamics In Shakespeare's The Tragedy Of Coriolanus And The Rape Of Lucrece, Lindsey Katherine Dee Wedow

Master's Theses

Early modern literature is replete with references to blood. These references appear in the contexts of class and gender distinction, medical information, religious significance, and more. Upon looking into the Galenic model of medicine utilized in early modern England, it becomes clear that blood, while one of the four humors of the body, held a place of special significance amongst the rest. Thus in reading the works of Shakespeare we are able to see how he often handles blood as a substance that holds special qualities and as such plays an important role in human lives. This paper examines two …

Neural Correlates Of Inhibitory Function Following The Implicit Processing Of Emotional Faces, Lorri A. Kais

Master's Theses

Emotion and cognitive function interact to play a central role in determining

human thought and behavior. Attention to emotion can facilitate or hinder cognitive

control efforts based on the given contextual demands of the task at hand. This study used

scalp electroencephalography (EEG) methods to examine the link between valence of

facial stimuli and neural changes associated with emotional face processing and

subsequent inhibitory response. 20 participants completed a gender discrimination stop-

signal task using emotional faces. Facial valence did not differentially modulate the P200

event-related potential (ERP), indicating that happy and sad faces recruit similar neural

resources in the …

The Effects Of Stress And Alcohol On Hiv-1 Latency, Sarah Ilene Talley

Master's Theses

A major barrier to HIV eradication is the persistence of latent viral reservoirs that exist despite antiretroviral therapy. In order to develop effective therapeutics, a comprehensive understanding of latency and factors driving the formation of the latent reservoir is needed. As stress and alcohol are common comorbidities associated with HIV infection, the goal of this research was to determine how stress and alcohol could affect HIV-latency. Specifically, we hypothesized that the ability of latent proviruses to be reactivated by “shock and kill” approaches would be altered by glucocorticoid and ethanol treatments, and prolonged ethanol exposure would affect the size of …

Utilizing Galectin 3 As A Marker Of Ruptured Vesicles In Vivo, Jonathan London

Master's Theses

Ruptured vesicles have been associated with pathological forms of α-synuclein during the development of PD. Galectin 3 (Gal3) is readily recruited to these ruptured vesicles, making it a potentially useful marker of vesicle rupture in PD and other diseases associated with vesicle rupture and lysosomal dysfunction. We were able to visualize and quantify the differences in Gal3 levels during infection with pathological α-synuclein in the rat brain. Having significantly high basal levels of Gal3, we found that the differentiated monocytes showed a nearly100 percent increase in intercellular levels of Gal3 after treatment with pathological α-synuclein. By expressing only the Carbohydrate …

Characterization Of Exopolysaccharide (Eps) Produced By Bacillus Subtilis Mutants, Alexander Argianas

Master's Theses

Bacillus subtilis is a probiotic bacterium that can protect against the murine-

specific attaching and effacing (A/E) pathogen Citrobacter rodentium. C. rodentium displays many features observed with the human pathogen enteropathogenic E. coli (EPEC). Protection by B. subtilis is mediated by exopolysaccharide (EPS), which is encoded by the epsA-O operon that contains 15 genes. Disruption of one of these genes, epsH, which appears to encode a putative glycosyltransferase, leads to the loss of protective capacity by B. subtilis and by EPS. EPS is a major component of the B. subtilis biofilm and the ΔepsH mutant is unable to form a …

Role Of Heat Shock Proteins In Maintenance Of The Gut Barrier Following Burn Injury, Abigail Rhea Cannon

Master's Theses

Introduction: Burn injury remains a prominent clinical problem. Patients suffering from burns often succumb to secondary infectious complications leading to sepsis and widespread tissue injury ending in Multiple Organ Dysfunction. However, the mechanism behind the onset of these extraneous symptoms following burn injury is not fully understood. The integrity of the gut barrier is of critical importance as it harbors the largest bacterial reservoir in the body. Following burn injury, we observed a breakdown of the gut barrier resulting in increased gut leakiness and bacterial translocation. Under homeostatic conditions, heat shock proteins (HSPs) stabilize tight junction proteins. Particularly, HSP72 is …

Separating Autoimmune And Anti-Tumor Responses With Hsp70iq435a, James Mahon

Master's Theses

Vitiligo and melanoma are both melanocyte-derived diseases; vitiligo progresses through the autoimmune-targeted destruction of healthy melanocytes while melanoma results from uncontrolled proliferation of cancerous melanocytes. Treatment of vitiligo with HSP70iQ435A inhibits the DC-mediated auto-immune response against melanocytes in vitiligo mouse models; this raises the concern that treatment with this modified HSP may also inhibit natural anti-tumor responses against melanocyte-derived melanoma tumor cells.

We hypothesized that HSP70iQ435A prevents migration of melanocyte-antigen reactive T cells to the skin in a way that is mechanistically different from anti-tumor responses to melanoma and therefore would not have a negative effect on anti-tumor responses. We …

Anthropogenic Litter And Microplastic In Urban Streams: Abundance, Source, And Fate, Amanda Rae Mccormick

Master's Theses

The accumulation and ecological effects of anthropogenic litter (AL) and microplastic are well-documented in marine ecosystems, but these materials’ abundances in rivers is unknown. AL enters rivers from recreation, runoff, and illegal dumping. Microplastic fibers and pellets are abundant in wastewater treatment plant (WWTP) effluent which enters rivers. Our objectives were to: (1) quantify AL abundance and composition in urban streams, (2) measure AL flux, accumulation, and export, and (3) measure riverine microplastic concentration and analyze its bacterial communities. We collected AL from 5 urban streams and observed greater AL density in riparian habitats and greater mass in benthic habitats. …

The Role Of Central Metabolism And Electron Transport In Biofilm Formation By Vibrio Fischeri, Jakob Michael Ondrey

Master's Theses

Biofilms protect bacteria from environmental threats, including antibiotics; thus, biofilms formed during infections pose an increasing threat to human health. A natural model used to study biofilm formation in the context of a host is the symbiosis between Vibrio fischeri and its host, the squid Euprymna scolopes. Successful colonization depends on the formation of a biofilm and genes involved in making the polysaccharide matrix component, syp. In culture, biofilm phenotypes, including the formation of wrinkled colonies, similarly depend on syp. However, little is known about other factors that contribute to this phenotype. To expand the utility of currently available genetic …

Malaria Transmission Blocking Vaccines, Lukasz Jacek Sewera

Master's Theses

Malaria affects 198 million people and kills 584,000 each year, predominantly in Sub-Saharan Africa (WHO). The most severe form of malaria is caused by the protozoan parasite Plasmodium falciparum. Development of a vaccine against P. falciparum has been hindered by its complex life cycle with multiple antigenically distinct human and mosquito stages. To effectively prevent disease and reduce the parasite burden in populations, a vaccine will need to target multiple stages, including blocking transmission at the mosquito stage.

Antibodies generated against P. falciparum mosquito stage antigen Pfs25 can prevent parasite transmission from humans to mosquitoes. However, Pfs25 is poorly immunogenic …

The Influence Of Eastern Oyster (Crassostrea Virginica) Reef Restoration On Nitrogen Cycling In A Eutrophic Estuary., Michael Hassett

Master's Theses

Oysters (Crassostrea virginica) are functionally extinct in the urbanized Hudson-Raritan estuary (HRE) in New York City, however, oyster restoration is promoted to mitigate nitrogen (N) pollution via oysters' filtration and excretion. Seasonally, I took 12 sediment cores (45 cm2) adjacent to and 10 m away from a recently constructed reef in the HRE. Cores were incubated in flow-through chambers with site water containing (1) no amendments, (2) 15N-ammonium, or (3) 15N-nitrate, from which I calculated coupled nitrification-denitrification and direct denitrification as isotope-enriched N2. Coupled denitrification was minimal at all sites while direct denitrification was elevated near the reef, suggesting organic …

Investigating The Role Of Cysteine In Biofilm Formation By Vibrio Fischeri, Priyanka Abhishek Singh

Master's Theses

Biofilms are an important area of medical research because bacterial biofilms can form on implants such as catheters and are highly resistant to antibiotics and other antimicrobials. The symbiosis between Vibrio fischeri-Euprymna scolopes, is good model to study biofilm. V. fischeri has 18 gene polysaccharide locus, syp, that is required for biofilm formation and colonization. A recent mutant study suggested the possibility that cysteine biosynthetic genes were involved in biofilm formation by V. fischeri, including cysH, cysJ, cysK, and cysN. My results supported a role for cysK and, to a lesser extent, cysH in biofilm formation. Finally, I have data …

Effects Of Stress On 9-1-1 Call-Takers And Police Dispatchers: A Study At The San Jose Police Department, Kimberly D. Turner

Master's Theses

This research addresses the significant gap in literature related to stress among 9-1-1 call-takers and dispatchers. The relationship between perceived stress and work-life balance, fairness at work, support at home, and work control as well as physiological and psychological outcomes is examined. Survey responses were collected during the summer of 2012 from 89 police 9-1-1 call-takers and radio dispatchers of the San José Police Department Communications Division in San José, California. Using hierarchical multiple regression analysis, I found those who enjoy a balance between work and personal life experience less stress. Also, greater stress is associated with more psychological and …

Tales Of Archaeological Places: Visualizing Mayan Pre-Colonial Settlement Patterns, Angela Ivanov

Master's Theses

In order to visualize ancient Mayan settlement patterns and demography through time, data derived from 26 published archaeological sites were collected and plotted onto three discrete map media. These data were subdivided into six time periods ranging from Pre-Classic to Post-Classic, and were simulated for each map medium. Specific attention was given during the selection of geographical locations, natural landscape features, map annotation, layers and coordinates. Tabulated information were site names, coordinates, site founding dates, site decline/abandonment dates, and estimated population. Combined with existing country base map layers downloaded from online geographical sources and time series animation (an animated map), …

Walt Kuhn: From Spotlight To Studio, The Art Of Show Business, Emily May Mcewan-Upright

Master's Theses

Walt Kuhn, an American artist born in New York, is often overlooked for his contribution to Modern American Art. Kuhn is typically recognized for his organizational and curatorial role in the 1913 Armory Show, but he was also internationally known in his time for his paintings of show people and as a creative director for several vaudeville and circus acts over his lifetime. During his career as a creative director, Kuhn’s artwork evolved from superficial renderings of theatre scenes to psychologically intense tronies of performers. Kuhn and other American artists, including the Ashcan group and Edward Hopper, examined the relationship …

"They Going To Throw You In Jackson Where You Belong": Depictions Of Mental Disability In The Works Of William Faulkner And Flannery O'Connor, Marie Elizabeth Lawson

Master's Theses

Mentally disabled characters in the works of William Faulkner and Flannery O’Connor are often subjected to mistreatment, neglect, and violence at the hands of their families and communities. In William Faulkner’s The Sound and the Fury (1929) and The Hamlet (1940) and Flannery O’Connor’s “The Life You Save May Be Your Own” (1955) and The Violent Bear it Away (1960), characters with mental disabilities are depicted in such a way as to evoke sympathy for the characters without the narratives overtly calling for sympathy. This is achieved through the juxtaposition of positive narrative portrayals with negative treatment by the characters’ …

Epigenetic Regulation Of Nuclear Hormone Receptor Dax-1, Michael B. Heskett

Master's Theses

DAX-1 (NR0B1) is an orphan nuclear receptor that plays a key role in the development and maintenance of steroidogenic tissue in mammals. Dax-1 is also expressed in mouse embryonic stem (ES) cells and is required to maintain pluripotency. Duplication of the X-chromosome in the region containing the NR0B1 gene results in sex reversal, and mutations in NR0B1 cause adrenal hypoplasia congenita. DAX-1 has been observed to act as a corepressor of other nuclear receptors including androgen receptor (AR), estrogen receptor (ER), and steroidogenic factor 1 (SF-1). In addition to pluripotent ES cells, DAX-1 is primarily expressed in select tissues of …

The Effects Of Robot-Child Interactions On Interpersonal And Intrapersonal Coordination, Kinga Palatinus

Master's Theses

Coordination is crucial throughout development, from motor development and language acquisition to children’s play and other social interactions. Based on the links between motor and social behavior, proper coordination should help children connect socially with others. Being part of a larger study investigating the effects of a multisystem intervention tool for children with ASD, this work examined the effect of robot-child interaction on interpersonal and intrapersonal coordination in typically developing children. 5-year-olds and 7 year-olds participated in a four-week training protocol, where children had to copy the movements of a small humanoid robot. Measures of cross recurrence quantification analysis revealed …

Bioremediation And Phytoremediation Systems For Breaking Down Total Petroleum Hydrocarbons (Tph) In Contaminated Sandy Soil, Patrick Mcintosh

Master's Theses

Bioremediation and Phytoremediation Systems for Breaking Down Total Petroleum Hydrocarbons (TPH) in Contaminated Sandy Soil

ABSTRACT

The applications of bio- and phytoremediation systems were used to breakdown low-level total petroleum hydrocarbons (TPH) in a contaminated sandy soil. The overall aim was to determine which soil regimes, amendments, and plant species enhance the breakdown of TPH. The first project included planted mixtures of cool and warm-season grasses with different willows, mono and intercropped with various fertilizer and carbon additions. The second project introduced different soil regimes of saturation, intermittent oxygenation, installment of aboveground covers, as well as the addition of switchgrass …

Into The Red: A Look Into The Reasons Why Refugees Decide To Flee, Settle Or Migrate To And From Morocco, Fadeelah E. Holivay

Master's Theses

This research paper explores some of the main reasons why refugees and asylum seekers, particularly from sub-Saharan African countries, embark on a journey and decide to settle, flee or migrate to and from Morocco. Because of this phenomenon, Morocco has seen a 96% increase of refugees migrating to the borders of Morocco each year for the past three years. Many say that this astonishing increase of migrants choosing Morocco is due to such factors as: wars breaking out regionally across central African and Middle Eastern countries causing them to flee; Morocco being a culturaly diverse francophone country whose laws and …