Digital Commons Network^™

Comparison Study Between Post-Fermentation Stillage And Pre-Fermentation Mash Utilizing Acidification And Steam Explosion Techniques For Cellulose Saccharification, Daniel Mckee

Master's Theses

Pre-fermentation mash fiber and post-distillation stillage fiber were examined and compared using a variety of preparatory techniques to determine the better source for cellulose fiber saccharification. Once screened, dried, and diluted to a 10% solution, mash fiber and stillage fiber were exposed to increasing temperatures for steam explosion techniques as well as increasing acidification techniques. Both underwent enzymatic saccharification to convert the exposed cellulose to glucose and other sugars. Once the optimum steam explosion technique parameters and acidification parameters were determined to be 2.5% sulfuric acid at 127.8°C for 1 hour, a comparison of the saccharification of pre-fermentation mash fiber …

Combined Effects Of Dissolved Oxygen And Temperature On Aerobic Respiration And Respiratory Recovery Responses Of The Spioniform Polychaete, Streblospio Gynobranchiata, In Relation To Body Size, Alyssa Bennett

Master's Theses

Elevated surface temperatures exacerbate the threat of hypoxia within coastal ecosystems. These two primary stressors likely interact as they elicit opposing physiological responses from marine organisms. Metabolic depression is typically associated with hypoxia, while metabolic rates increase with temperature. Moreover, physiological effects of combined stressors may not be additive. In light of increasing pressures from hypoxia, elevated ocean temperatures, and other stressors within coastal regions, studies need to examine effects of multiple stressors on physiology of coastal organisms.

Mass-specific aerobic respiration (V_O2) was characterized as a proxy for metabolic cost of Streblospio gynobranchiata, at combined levels of …

Sex Differences In Collateral Remodeling Following Hindlimb Arterial Occlusion, Laura Burckhardt

Master's Theses

Clinical evidence indicates a higher incidence of peripheral arterial occlusive disease and associated likelihood of critical limb ischemia in women, as well as worse prognosis and decreased survival post myocardial infarction. Therefore, understanding the possible differences in underlying vascular compensation mechanisms is crucial. With arterial occlusions, necrosis and tissue injury can be naturally mitigated by the collateral circulation, improving patient prognosis. Previous sex-comparison studies describing differences in vascular remodeling are inconsistent. Therefore, the aim of this study was to describe the effect of arterial occlusion on collateral remodeling in healthy male and healthy reproductive-stage female mice. At 7 days following …

Influences Of Anthropogenic Noise On Flight Initiation Distance, Foraging Behavior, And Feeder Community Structure Of Wild Birds, Alissa R. Petrelli

Master's Theses

Throughout the world, birds represent the primary type of wildlife that people experience on a daily basis. However, a growing body of evidence suggests that alterations to the acoustic environment can negatively affect birds as well as humans in a variety of ways, and altered acoustics from noise pollution has the potential to influence human interactions with wild birds. In this thesis, I investigated how anthropogenic noise impacts daily behavior as well as community structure of wild birds. In the first component of this thesis, I assessed the distance at which a bird initiates flight or escape behavior (i.e., flight …

Improving Companion Ai Behavior In Mimica, Daniel Toy

Master's Theses

Companion characters are an important aspect of video games and appear in many different genres. Their role is typically to support the player as they progress through the game by helping to complete tasks or assisting in combat. However, oftentimes, these companion characters are limited in their ability to dynamically react to new situations and fail to properly assist the player.

In this paper, we present a solution by improving upon the MimicA framework, which allows companion characters to emulate the human player. The framework takes a learn by observation approach by storing the game state when the player performs …

A Neuroprotective Role For Mir-1017, A Non-Canonical Mirna, Matthew De Cruz

Master's Theses

miRNAs are post-transcriptional regulators of gene expression, with numerous being involved in neurobiology. Within the human genome a quarter of the identified miRNA loci derive from a class of miRNAs termed tailed mirtrons. Despite the identification of this large population of miRNA, no functional studies have been conducted to identify their role. In this study we examined the highly expressed and deeply conserved Drosophila 3’ tail mirtron, miR-1017, as a candidate to elucidate tailed mirtron functionality. We identified acetylcholine receptor transcripts, Da5 and Da2, as bona fide targets for miR-1017. Interestingly, Da2 is also the host transcript for miR-1017. We …

Effects Of Temperature On Growth And Molting In Blue Crabs (Callinectes Sapidus) And Lesser Blue Crabs (Callinectes Similis), Abigail Ann Kuhn

Master's Theses

Temperature can exert impacts on many processes in ectotherms. With global temperatures rising due to climate change, many ectothermic species may exhibit changes in growth rates and size at maturity, and these changes can have population-level effects. Predicting responses of species to climate change will require not only knowledge of thermal tolerance limits, but also effects of temperature change on growth rates and other life history parameters. For arthropods that exhibit discontinuous growth (i.e., molting), this includes both intermolt period and growth per molt. Previous laboratory and field experiments suggest that temperature affects both intermolt period (IMP) and growth per …

Impacts Of Oil Exposure During Early Life Development Stages In Sheepshead Minnows (Cyprinodon Variegatus) Under Different Environmental Factors, Danielle Simning

Master's Theses

The release of approximately 5 million barrels of crude oil into the northern Gulf of Mexico during the 2010 Deepwater Horizon oil spill jeopardized estuarine ecosystem health from Texas to Florida. These estuarine habitats, which serve as nurseries for many important fisheries are also prone to rapid fluctuations in environmental stressors such as oxygen concentration, and salinity. The consequence of combined exposure to crude oil and suboptimal environmental factors during early life stage development of fish is still largely unknown. The objective of this project was to investigate the impacts of exposure to crude oil in combination with varying environmental …

Examining Movement Dynamics Of The Gulf Menhaden Fishery Using An Individual-Based Model, Robert D. Trigg Iii

Master's Theses

This study investigates the movement and harvest dynamics of the Gulf Menhaden Brevoortia patronus fishery. The fishery-dependent data collected by NOAA (years 2006-2009 and 2011) describe vessel-specific information on catch locations (latitude and longitude) and magnitude of harvest in metric tons (mt). A series of probability distribution functions (PDFs) were fit to the frequency distributions of number of harvests per day (Poisson), between-harvest distances (gamma), and harvest magnitude (log-normal). These analyses were used to inform an individual-based model (IBM). The IBM was run under several different spatial restriction regimes, including (1) current regulations in Texas, Louisiana, Mississippi, and Alabama; (2) …

The Influence Of Talker Expectations And Acoustic Variability On Speech Perception In Asd, Anders Hogstrom

Master's Theses

Speech perception is dependent upon the ability to map the sensory features of a speech signal onto the perceptual features which make up language (i.e., phonemes). A great deal of research over the past six decades has focused on how variability across talkers influences speech processing. Listeners are required to normalize the acoustic variability across talkers by continuously updating the mapping from the acoustic signal to phonetic representations. As such, processing speech from multiple talkers is cognitively more demanding than listening to a single talker. This processing cost appears to reflect, in part, the influence of listeners’ expectations that speech …

Immunohistochemistry And Electron Microscopy Of Rat Palatine Glands Development, Zaki Hakami

Master's Theses

No abstract provided.

Identifying And Reducing Variability, Improving Scaffold Morphology, And Investigating Alternative Materials For The Blood Vessel Mimic Lab Electrospinning Process, Evan M. Dowey

Master's Theses

The work of the Cal Poly Tissue Engineering Lab is primarily focused on the fabrication, characterization, and improvement of “Blood Vessel Mimics” (BVMs), tissue engineered constructs used to evaluate cellular response to vascular medical devices. Currently, cells are grown onto fibrous, porous tubes made using an in-house electrospinning process from PLGA, a biocompatible co-polymer. The adhesion and proliferation of cells in a BVM is reliant on the micro-scale structure of the PLGA scaffold, and as such it is of great importance for the electrospinning process to consistently produce scaffolds of similar morphologies. Additionally, it has been shown that cell proliferation …

Proteomic Analysis Of The Crustacean Molting Gland (Y-Organ) Over The Course Of The Molt Cycle, Talia B. Head

Master's Theses

Molting in crustaceans is a highly complex physiological process involving negative regulation by two paired endocrine glands, the X-organ/sinus gland complex (XO/SG) and the Y-organ (YO). The XO/SG complex is responsible for making molt-inhibiting hormone (MIH) which negatively regulates synthesis of the molting hormones, ecdysteroids, by the YO. Analysis of gene expression in the XOs and YOs has led to the development of a proposed molecular signaling pathway which regulates ecdysteroidogenesis and subsequent molting in crustaceans. In this study, changes in protein abundance in the YO were characterized over the course of a molt cycle (intermolt, early premolt, mid premolt, …

Sex Differences And The Effects Of Exercise Training On Functional Vasodilation Following Arterial Occlusion In The Balb/C Mouse Spinotrapezius, Britta Nelson

Master's Theses

Peripheral arterial occlusive disease (PAOD) often presents as intermittent claudication, which may be caused by impaired vasodilation. Impairment of resistance vessels may contribute to the pathogenesis of PAOD, and explain the poor correlation between resting blood flow and limb function. Collateral function following arterial occlusion is not well defined, however collaterals and arterialized collateral capillaries (ACCs) in male and female animal models exhibit impaired vasodilation following arterial occlusion, which can potentially be improved with exercise training. Furthermore, resistance vessels in the ischemic tree and stem are likely involved in the pathogenesis of PAOD, however the relative importance of each is …

X Chromosome Dosage Compensation And Gene Expression In The Sheep, Kaleigh Flock

Master's Theses

No abstract provided.

Key Differences Between Hepatic And Intestinal Apoc3 Regulation And Expression, And Implications For Cardiovascular Disease Risk, Gabrielle West

Master's Theses

In the intestine, apoC-III delays chylomicron secretion, and causes a decrease in chylomicron size. Once apoC-III is in circulation, apoC-III delays triglyceride-rich remnants clearance by inhibiting lipoprotein lipase and liver low-density lipoprotein receptor. In humans, high levels of plasma apoC-III directly result in hypertriglyceridemia. ApoC-III is a critical cardiovascular risk factor, and humans expressing null mutations in apoC-III are robustly protected from cardiovascular disease. Because of its critical role in elevating plasma lipids and CVD risk, the factors that regulate apoC-III expression in the liver have been determined, and include glucose, insulin (through FoxO1), and dietary fat. Considerably less is …

Investigating The Physiological And Behavioral Responses To Fear In A Virtual Reality Fear Conditioning Paradigm, Emily Errante

Master's Theses

No abstract provided.

Entap: Software To Improve The Quality And Functional Annotation Of De Novo Assembled Non Model Eukaryotic Transcriptomes, Alexander Hart

Master's Theses

EnTAP (Eukaryotic Non-Model Transcriptome Annotation Pipeline) was designed to improve the accuracy, speed, and flexibility of functional gene annotation for de novo assembled transcriptomes in non-model Eukaryotes. This software package addresses the fragmentation and related assembly issues that result in inflated transcript estimates and poor annotation rates. Following filters applied through assessment of true expression and frame selection, open-source tools are leveraged to functionally annotate the translated proteins. Downstream features include fast similarity search across three repositories, protein domain assignment, orthologous gene family assessment, and Gene Ontology term assignment. The final annotation integrates across multiple databases and selects an optimal …

The Role Of Bmp Signaling In Mediating Stimulatory Effects Of Fgf-Fgfr Signaling On Odontoblast Differentiation In Dental Pulp, Karen Sagomonyants

Master's Theses

Odontoblast differentiation is dependent on multiple signaling molecules, including growth factors stored in the extracellular dentin matrix. Our previous studies have demonstrated that Fibroblast Growth Factor 2 (FGF2) exerted stage-specific effects on odontoblast differentiation in primary dental pulp cultures. FGF2 stimulated differentiation of functional odontoblasts expressing high levels of Dmp1 from early progenitors but inhibited the terminal differentiation of functional odontoblasts to fully differentiated odontoblasts expressing high levels of Dspp. These stimulatory effects involved activation of FGFR and ERK1/2, and increased levels of expression of Bmp2, Runx2, and Osx (components of BMP signaling) suggesting the involvement of …

Effects Of A Standardized, Freeze-Dried Grape Powder In Adults With Metabolic Syndrome, Quinn Duclos

Master's Theses

Diets rich in fruits and vegetables have been correlated to lower disease risk. This is thought to be due to bioactive components such as: vitamin C, vitamin E, carotenoids, and polyphenols. Grapes are a food source of many bioactive components, including polyphenols. The effects standardized, freeze-dried, grape powder (GRAPE) were examined in adults with metabolic syndrome (MetS). Twenty men and women (aged 30-70 y) were instructed to consume 60 g/day of GRAPE (equivalent to 2.5 cups fresh grapes daily) or nutritionally-matched placebo powder in a randomized, placebo-controlled, crossover intervention study for 13 weeks. This included 2-week run-in, 4-week intervention (GRAPE …

Textural Analysis Of Historical Aerial Photography To Determine Change In Coastal Marsh Extent: Site Of The Present-Day Grand Bay National Estuarine Research Reserve (Gbnerr), Mississippi, 1955-2014, Heather Michelle Nicholson

Master's Theses

Coastal marshlands are among the world’s most highly productive ecosystems but they have diminished greatly in the past several decades owing to sea-level rise and direct anthropogenic influences. An effective means of quantifying loss or gain in marsh area is through the use of aerial image data, which offers synoptic views of the landscape at decadal-scale sampling frequencies. However, a potential problem with older panchromatic, or black-and-white, imagery is the absence of multispectral information that might be used otherwise in remote identification of vegetation types. Nevertheless, the analysis of horizontal variability in image brightness values, or image texture, can be …

Solitary And Social Object Play In The Bottlenose Dolphin (Tursiops Truncatus), Briana M. Cappiello

Master's Theses

Many comparative studies have focused on the emergence of solitary and social play in early development. However, few consider observer and parallel forms of solitary play. In this study, use of video analysis revealed that there is a significant difference between the frequency of solitary, observer, parallel, and social object play states, in calf, juvenile, subadult, and adult bottlenose dolphins (Tursiops truncatus). While juveniles engaged in solitary play significantly more than any other age group, both juveniles and calves engaged in observer and social play significantly more than adults and subadults. Within their own age group, calves, juveniles, …

The Differential Expression Of Gene Cohorts Over The Course Of Development In Type I And Type Ii Layer V Pyramidal Neurons Of The Mouse Medial Prefrontal And Primary Somatosensory Cortex, Ryan Shaun Pevey

Master's Theses

Two of the most challenging problems in neuroscience may be the characterization of different neuron types and determining the various decision points over the course of development that defines those different cell types. Part of the problem is that for the clear majority of neuron types, no single gene so far has been shown to act as a type marker. Instead it seems that signatures involving multiple genes are necessary to define each type. The identity of the genes in these signatures remains an important gap in our knowledge. However, recent developments in bioinformatics have produced population level transcript sequencing …

How Did We Get Here? An Inquiry Into Pop Culture, Pop Culture Icons, And America's Support Of The Degradation Of Humanity Through Mass Media Consumption, Thomas Steele

Master's Theses

This thesis focuses on the contemporary human condition associated with the consumption of current entertainment media. Sampling on modes of thought from anthropological, biological, psychological, sociological, and biographical points of view I sought to define what it means to be uniquely human. Furthermore, I sought to compare and contrast contemporary human interactions as described through research studies associated with tabloid news sources, and the entertainment industry. The results of this study were then articulated through four works of art that visually describe that which was found amongst a variety of resources. They describe the rise and fall of the careers …

Biodiversity And Phenology Of The Epibenthic Macroinvertebrate Fauna In A First Order Mississippi Stream, Jamaal Bankhead

Master's Theses

I used Hester-Dendy multiplate samplers and stick and leaf samples to collect the aquatic insects of Granny Creek, a first-order perennial stream in southern Mississippi, on twelve sampling dates from March 2014 to June 2015. The dominant insect/invertebrate group in Granny Creek was the dipteran family Chironomidae. Forty-two distinct taxa of chironomid larvae were collected over the duration of my study. Two of the dominant chironomid subfamilies in my samples, the Orthocladiinae and the Chironominae, displayed a phenological pattern in which the Orthocladiinae were more prevalent, both in terms of number of species and in abundance of individuals, in the …

Potential Of Ozone And Hydroxyl Radicals To Degrade Emerging Organic Contaminants, Lisa Elaine Vance

Master's Theses

Advanced water treatment processes are necessary for the treatment of emerging organic contaminants. Traditional treatment processes do not filter or treat these contaminants causing humans and animals to come into contact and ingest them, both causing illness. Advanced oxidation processes are a good alternative method, but are cost and time intensive.

Previous research on ozone and hydroxyl radicals leads to the conclusion that they might be a vial option for advanced water treatment processes. To test the effects of ozone and hydroxyl radicals on the destruction of algae, ozonated phosphate buffer was mixed with Anabaena and modified BG11 culture media …

Investigation Of Ammonia And Nitrate Removal From Municipal Wastewater Using Biowish, Emily Holland

Master's Theses

This research entails investigation of ammonia, nitrate, and nitrite removal from wastewater using a proprietary blend of bacteria known as BiOWiSH^TM. The degradation rates of ammonia, nitrate, and nitrite for Aqua were determined using wastewater at the San Luis Obispo Water Resource Recovery Facility (SLO WRRF). Laboratory and field experiments were conducted to test how Aqua compared with natural bacteria for removal of nitrogen compounds. Preliminary data suggested that Aqua performed nitrate removal best in SLO WRRF wastewater at the secondary clarifier. Aqua could perform anoxic and aerobic denitrification in secondary clarifier wastewater. In mineral media, Aqua removed …

Removal Of Nitrates From Stormwater Using Nanoclays, Rubia Siddiqi

Master's Theses

Creeks and rivers are often polluted as a result of stormwater runoff that carries various contaminants in to open water bodies, causing adverse environmental and health effects. Low impact development (LID) techniques are currently employed to treat this runoff prior to discharge. Nitrate, however, is not consistently removed by these LID techniques. This study analyzed the ability of several nanoclays to remove nitrate in runoff and determined the feasibility of using them as a soil supplement for LID implementation. Six different nanoclays and HCl-treated clays were compared (pre-modified trimethyl stearyl ammonium nanoclay, pre-modified dimethyl dialkyl amine nanoclay, unmodified hydrophilic bentonite, …

Effects Of Wave Action On The Structure Of Fish Assemblages Across An Exposure Gradient, Lauren Liddon

Master's Theses

Disturbance affects the function and diversity of ecosystems. Increased wave exposure to salt marsh can disturb sediments and cause a loss of habitat. The purpose of this study was to explore the effects of increased wave exposure on diversity, abundance, and functional ecology of estuarine fishes. If increased wave exposure is acting as a disturbance to these habitats, ecological theory (Intermediate Disturbance Hypothesis) predicts that diversity will peak at intermediate frequencies and intensities of disturbance. Fish were sampled from 10 sites monthly for 6 years. The sites were assigned to different exposure categories (Open, Intermediate, and Sheltered) using an exposure …

Sedimentary Characteristics And Nutrient Sequestration Of Embanked Floodplains Along The Lower Mississippi River, Mississippi And Louisiana, R M Malitha Rathnayake

Master's Theses

The Mississippi River Basin is the largest river basin in North America and the third largest river basin in the world. Most of the corn, soybeans, wheat, cattle, and hogs harvested in the United States come from the Mississippi River Basin and about 58% of the entire drainage basin is croplands. Runoff from these lands carries sediments and nutrients, and the Mississippi River transports these downstream and ultimately deposits them in the Gulf of Mexico. The northern Gulf of Mexico is one of largest human-caused hypoxic zones in the world. Hypoxia is the phenomena where the dissolved oxygen level decreases …