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LSU Historical Dissertations and Theses

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Environmental Factors And Production Characteristics Affecting The Culture Of Red Drum, Sciaenops Ocellatus., Rowland Glenn Thomas Jan 1991

Environmental Factors And Production Characteristics Affecting The Culture Of Red Drum, Sciaenops Ocellatus., Rowland Glenn Thomas

LSU Historical Dissertations and Theses

Experiments with red drum, Sciaenops ocellatus, were conducted to assess environmental requirements and culture characteristics. Pond trials were used to evaluate the effects of salinity, chloride source, predator control, acclimation procedure, stocking density, and size at stocking on production. Survival in individual ponds ranged from 0-92%; average daily weight gain from 0.46-2.22g; and feed conversion ratio from 2.30-4.90. Survival, growth, and feed conversion ratio did not differ significantly between equivalent treatments in saltwater and freshwater ponds. The minimum chloride concentration necessary for acceptable production of red drum in freshwater ponds (250-300 mg/l) was at least 100% higher than the concentrations …

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The Morphology, Development, And Function Of Experimentally Induced Macrophage Aggregates In Rivulus Marmoratus., Wolfgang Klaus Vogelbein Jan 1991

The Morphology, Development, And Function Of Experimentally Induced Macrophage Aggregates In Rivulus Marmoratus., Wolfgang Klaus Vogelbein

LSU Historical Dissertations and Theses

Calyptospora funduli is a pathogenic coccidium that infects the liver and pancreas of estuarine killifishes belonging to the genus Fundulus. Experimental infection of the atypical fish host Rivulus marmoratus with this intracellular protozoan parasite caused the development of hepatic macrophage aggregates (MA's) and granulomas. The Rivulus/Calyptospora host-parasite system thus represents a new laboratory model for investigations of MA biology. In the present study, this model was used to: (1) examine the morphology and sequential development of the parasite-elicited hepatic MA's in R. marmoratus, (2) define the role that these structures play in the pathogenesis of an infectious disease process, and …

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A Quest For Legitimacy: Impression Management Strategies Used By An Organization In Crisis., Rachel Harris Caillouet Jan 1991

A Quest For Legitimacy: Impression Management Strategies Used By An Organization In Crisis., Rachel Harris Caillouet

LSU Historical Dissertations and Theses

The purpose of this study was to investigate impression management strategies used by an organization in crisis and to develop a typology of impression management strategies used by an organization. The focus of the study was Marine Shale Processors (MSP), an environmental company located in Amelia, Louisiana, which recycles hazardous and non-hazardous materials into a non-toxic aggregate. Impression management strategies used by MSP in statements to stakeholders were examined using multiple methods. Also, the study examined whether or not different strategies were utilized with different stakeholders, whether or not management and non-management relied on the same strategies with stakeholders, and …

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A New Construction Of Subgroups Inducing Isomorphic Representations., Patricia Wright Beaulieu Jan 1991

A New Construction Of Subgroups Inducing Isomorphic Representations., Patricia Wright Beaulieu

LSU Historical Dissertations and Theses

This dissertation provides a method to construct infinite families of triples ($H\sb{i},H\sbsp{i}{\prime},G\sb{i}$) consisting of two non-conjugate subgroups $H\sb{i}$ and $H\sbsp{i}{\prime}$ of a finite group $G\sb{i}$ whose trivial representations induce isomorphic representations of $G\sb{i}$. Such triples are important in number theory, differential geometry, and group theory.

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Distribution And Management Of And Soybean Resistance To Calonectria Crotalariae, The Causal Pathogen Of Red Crown Rot Of Soybean., Dana Kent Berner Jan 1991

Distribution And Management Of And Soybean Resistance To Calonectria Crotalariae, The Causal Pathogen Of Red Crown Rot Of Soybean., Dana Kent Berner

LSU Historical Dissertations and Theses

The national, local, and in-field distribution of Calonectria crotalariae (Loos) Bell and Sobers, as reflected in the distribution of Cylindrocladium black rot (CBR) of peanut, red crown rot (RCR) of soybean and microsclerotia densities in soil, respectively, are described. The current distribution of RCR in Louisiana is probably due to the introduction of the pathogen on indigo (Indigofera tinctoria (L.)) and to subsequent movement by man. RCR has been reported in 17 Louisiana parishes. In field studies, delayed soybean planting was efficacious in reducing RCR incidence. Effects of delayed planting on yield were maturity group dependent and confounded with RCR …

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Partial Sequence And Characterization Of A Growth Hormone Gene Of The Red Drum (Sciaenops Ocellatus)., Carol Marie Dorworth Jan 1991

Partial Sequence And Characterization Of A Growth Hormone Gene Of The Red Drum (Sciaenops Ocellatus)., Carol Marie Dorworth

LSU Historical Dissertations and Theses

A growth hormone (GH) gene was isolated from nuclear DNA of the red drum (Sciaenops ocellatus). Synthetic oligonucleotides were synthesized based on published cDNA sequences of other perciform fishes. Polymerase chain reaction (PCR) methodology was employed to amplify a nucleotide sequence extending from the 5$\sp\prime$ end of intron I to 15 bases upstream of the 3$\sp\prime$ end of exon VI of the GH gene. Amplified DNA was cloned into a phagemid sequencing vector. Clones were sequenced. Computer analysis of sequence data was used to determine the internal gene arrangement of exons and introns. Homologies of the nucleotide and deduced amino …

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Effects Of Chronic Administration Of Phenylbutazone On Reproduction In The Mare., Mareth Ellsworth Jan 1991

Effects Of Chronic Administration Of Phenylbutazone On Reproduction In The Mare., Mareth Ellsworth

LSU Historical Dissertations and Theses

Phenylbutazone is a non-steroidal anti-inflammatory drug that acts by inhibiting prostaglandin synthesis. Prostaglandins are associated with crucial aspects of reproduction. In 5 experiments, mares were used to test the effects of phenylbutazone on reproduction. In all experiments, treatment was 2 g phenylbutazone or 10 ml physiological saline intravenously(control). In Experiment I, 9 mares were phenylbutazone-treated for 180 consecutive days and teased. Ovaries were palpated and cyclicity was verified by circulating progesterone concentrations. Thirty-six cycles were evaluated. Mares were then divided into treatment and control groups. Estrous mares were artificially inseminated. Non-surgical embryo collection was performed 7 to 8 days post-ovulation. …

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On Bubble Columns. (Volumes I And Ii)., Nicholas William Geary Jan 1991

On Bubble Columns. (Volumes I And Ii)., Nicholas William Geary

LSU Historical Dissertations and Theses

There exists a narrow operating window in bubble columns where the motion is so gentle that the normally dominating forces of coalescence and breakup no longer define column conditions. This behavior is synonymous with operation in the bubbly flow regime. During experimentation, two distinct hydrodynamic regions were observed; close to the sparger, the liquid was well mixed, whilst in the remainder of the column a dispersive flow model was determined to be appropriate. In the entrance zone, the isotropic turbulence theories of Kolmogoroff adequately represented transport behavior. This finding implies that a churn-turbulent environment, wherein bubbles are continuously reformed, will …

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Taxonomy And Biostratigraphy Of Sabinian Palynomorphs From The Wilcox Group (Paleocene-Eocene Epochs) Of Southwestern Louisiana., William Andrew Gregory Jr Jan 1991

Taxonomy And Biostratigraphy Of Sabinian Palynomorphs From The Wilcox Group (Paleocene-Eocene Epochs) Of Southwestern Louisiana., William Andrew Gregory Jr

LSU Historical Dissertations and Theses

Fifty conventional core samples from the Ragley Lumber #D1 well located in Allen Parish Louisiana (Sec 29, T5S, R7w) were analyzed for palynomorphs. The sediment was deposited during the Sabinian Provincial Stage (upper Paleocene-lower Eocene) in a marine environment as evidenced by the presence of in situ dinocysts and acritarchs in every sample. Terrigenous diversity of 129 taxa is comparable with marine palynomorph diversity of 46 taxa. Single sample diversity among the terrigenous palynomorph component (23-56 taxa) is comparable with single sample marine palynomorph diversity of 7-25 taxa. High terrigenous diversity intervals possibly indicate sourcing from multiple onshore environments or …

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Mechanism And Enhancement Of Phase Segregation In Drilling Fluids., John Marvin Griffin Jan 1991

Mechanism And Enhancement Of Phase Segregation In Drilling Fluids., John Marvin Griffin

LSU Historical Dissertations and Theses

Dewaterability of a compressible suspension can be characterized by two fundamental properties of viscoelastic networks: permeability and bulk modulus of elasticity. Time required for dewatering can be obtained from the former, while the latter can furnish the data required for predicting ultimate compaction. A method has been derived and an apparatus constructed for measurement of these two properties in a centrifugal field. The method was applied to a variety of drilling fluids and mineral suspensions. A floor-mounted laboratory centrifuge was used in this investigation. The methods developed have merit for evaluating pre-treatments prior to centrifugal dewatering. A method for quantifying …

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Comparative Inflorescence And Floral Development In The Asteraceae. (Volumes I And Ii)., Elizabeth M. Harris Jan 1991

Comparative Inflorescence And Floral Development In The Asteraceae. (Volumes I And Ii)., Elizabeth M. Harris

LSU Historical Dissertations and Theses

Floral and inflorescence development of 39 species of Asteraceae and four additional species representing possible outgroups were examined with the scanning electron microscope (SEM). Taxon choice was designed to include representatives of 12 of the 12 to 18 tribes recognized in the large, morphologically diverse family. Although floral ontogeny has provided phylogenetically useful characters in other plant groups, no similar studies have been applied to solve phylogenetic problems in the Asteraceae. The necessary live material was collected in several ways: from natural populations throughout North America, from the worldwide collections of several botanical gardens, grown from seed, or from cultivated …

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Radial Flow Chromatography In Compressed Pancake-Shaped Beds., Brian Keith Heft Jan 1991

Radial Flow Chromatography In Compressed Pancake-Shaped Beds., Brian Keith Heft

LSU Historical Dissertations and Theses

The separation of a methane/n-butane mixture was demonstrated using radial flow through compressed, pancake-shaped beds of fine alumina powder. The compacted stationary phase improved performance relative to an uncompressed sorbent by assuring flow uniformity through the bed, reducing band-broadening and tailing (by decreasing interstitial hold-up) and decreasing effective plate height (through an increase in the capacity ratio of the chromatograph). Dispersion was identified as the key dissipative effect in the prototype radial flow adsorber. An exact analytical solution derived for the transport equations describing such a device compared favorably with experimental data. The dispersion coefficients were found to be strong …

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Isolation And Characterization Of Macrophage Inhibitory Compounds From Mycobacterium Paratuberculosis., Murray Elison Hines Ii Jan 1991

Isolation And Characterization Of Macrophage Inhibitory Compounds From Mycobacterium Paratuberculosis., Murray Elison Hines Ii

LSU Historical Dissertations and Theses

Fractions exhibiting activity detrimental to the killing function of activated bovine monocyte/macrophages towards Candida albicans were obtained from Mycobacterium paratuberculosis. Active fractions were initially derived by ultrasonication of bacterial samples followed by ammonium sulfate precipitation, ion-exchange chromatography, gel filtration chromatography, heat precipitation, acid precipitation and alkali treatment. Two different fractions that exhibited marked inhibition of macrophage killing ability were derived and partially characterized. Novel methodology was developed to accomplish the task of lysis and fractionation of mycobacteria utilizing the Matrix Solid Phase Dispersion (MSPD) technique that appeared to be much more efficient and is certainly less time consuming and less …

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The Effect Of Co-Culture Systems From Non-Reproductive Origins On The Development Of Superovulated And In Vitro Fertilized Bovine Embryos., Hak-Nam Kim Jan 1991

The Effect Of Co-Culture Systems From Non-Reproductive Origins On The Development Of Superovulated And In Vitro Fertilized Bovine Embryos., Hak-Nam Kim

LSU Historical Dissertations and Theses

The objective of this experiment was to compare in vitro development of bovine embryos obtained from superovulation and in vitro fertilization (IVF) in two unique monolayer co-culture systems, bovine fetal spleen cell monolayers (BFS) and chick embryo fibroblast monolayers (CEF). To test the possible harmful effects of these co-culture systems, 225 murine morula-stage embryos were cultured for 72 hours with these monolayers. The percentage of hatched embryos was 62.7% in culture medium alone, 76% in BFS and 57.3% in CEF. BFS and CEF did not induce any detectable harmful effect on these developing murine embryos. When 60 transferable morula-stage bovine …

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Ruminal Distribution And Utilization Of Zinc And Magnesium As Influenced By Dietary Source And Energy Level., Donald Wayne Kennedy Jan 1991

Ruminal Distribution And Utilization Of Zinc And Magnesium As Influenced By Dietary Source And Energy Level., Donald Wayne Kennedy

LSU Historical Dissertations and Theses

Steers fed a conventional diet were utilized to determine the effects of various Zn supplements on ruminal distribution of Zn. Treatments consisted of a basal (B) diet (no Zn supplementation) and B supplemented with equal parts of Zn from either a polysaccharide complex of Zn (PC-Zn) or zinc oxide (ZnO). Samples were fractionated into four fractions: a cell-free fraction, a particulate fraction and two microbial fractions. After PC-Zn or ZnO were dosed intraruminally, relative distribution of Zn from each Zn source within each fraction was evaluated up to 8 h postdosing. Cannulated growing lambs were used to study the effects …

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Fluorescence Studies Of Phosphofructokinase From Bacillus Stearothermophilus., Soon-Jong Kim Jan 1991

Fluorescence Studies Of Phosphofructokinase From Bacillus Stearothermophilus., Soon-Jong Kim

LSU Historical Dissertations and Theses

Phosphofructokinase catalyzes the phosphorylation of $\beta$-fructose 6-phosphate (Fru-6-P) to $\beta$-fructose 1,6-bisphosphate. The first part of this dissertation shows that phosphofructokinase from Bacillus stearothermophilus (Bs-PFK) is a tetramer in the region pH 4.5-9.5 unlike the enzymes from mammalian sources, which exhibit association-dissociation reactions. Circular dichroism, steady-state fluorescence, and static and dynamic light scattering were used to study the conformational properties of the enzyme. Inactivation of the enzyme below pH 7.0 is not due to dissociation of the tetramer to dimers or monomers. In the second part, the fluorescence of the lone tryptophan was investigated by steady-state and time-resolved techniques. The decay …

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Patterning The Past: History As Ideology In Modern Southern Fiction., Deborah Wilson Jan 1991

Patterning The Past: History As Ideology In Modern Southern Fiction., Deborah Wilson

LSU Historical Dissertations and Theses

In this study, I analyze the modes of historical representation in works by Robert Penn Warren, William Faulkner, Eudora Welty and Ellen Douglas. In the chapter on All the King's Men, a novel that exemplifies the masculine historical perspective of traditional Southern literature, I show how Warren defines history as a process moving toward a predetermined end and then structures the narrative so that the women characters are constantly positioned outside that definition. In the second chapter, I begin with Eudora Welty's The Robber Bridegroom, examining the ways she alters the traditional story line of American history by drawing attention …

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An Examination Of Auditor Changes Following Events Adversely Affecting External Auditor Credibility., Thomas E. Wilson Jr Jan 1991

An Examination Of Auditor Changes Following Events Adversely Affecting External Auditor Credibility., Thomas E. Wilson Jr

LSU Historical Dissertations and Theses

This study examines auditor changes following events adversely affecting auditor credibility. Disciplinary actions against auditors by the Securities and Exchange Commission (SEC) are posited to be events damaging to auditor credibility. Agency theory and prior research are used to generate hypotheses about differences between client firms that switch auditors and client firms that do not switch auditors after an SEC action under Rule 2(e) against their auditor. A series of univariate and multivariate statistical tests are conducted on the sample firms. The sample is divided into a Switch group and a Non-Switch group. The sample as a whole is examined, …

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Methyl Oleate Ozonides In Aqueous Micelles: Formation, Stability, And Initiation Of Lipid Peroxidation, And, Relative Antioxidant Efficiencies In Aqueous Micelles Of Anionic And Cationic Surfactants., Mingdan Wu Jan 1991

Methyl Oleate Ozonides In Aqueous Micelles: Formation, Stability, And Initiation Of Lipid Peroxidation, And, Relative Antioxidant Efficiencies In Aqueous Micelles Of Anionic And Cationic Surfactants., Mingdan Wu

LSU Historical Dissertations and Theses

Ozonation of methyl oleate has been shown for the first time to form ozonides in aqueous micelles of sodium dodecyl sulfate (SDS) and in L-$\alpha$-distearoyl-phosphatidylcholine (DSPC) liposomes. The total yield of ozonides in 0.02 M SDS micelles exposed to 26 ppm ozone at pH 7.4 and 22$\sp\circ$C is 11.1%. This 11.1% consists of 7.5% of the normal ozonide (MOO2), ca. 4.2% of trans-isomer and ca. 3.3% of cis-isomer, and the rest 3.6% is accounted for by the cross ozonides (MOO1 and MOO3). Regarding the hydrolytic stability of the ozonides under physiologically relevant conditions (e.g., pH 7.4 and 37$\sp\circ$C), the half-lives …

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Statistical Sophistication Of Research In Vocational Education., Chi Zhang Jan 1991

Statistical Sophistication Of Research In Vocational Education., Chi Zhang

LSU Historical Dissertations and Theses

The purpose of this study was to determine the status of, and changes in, the statistical sophistication of research in vocational education in the 1980s. The study also explored relationships among statistical sophistication, problem area studied, and methodological strategy used in vocational education research. Data collected were obtained from 118 quantitative research reports randomly selected from the Journal of Vocational Education Research, the Journal of Agricultural Education, the Journal of Industrial Teacher Education, and the Journal of Vocational Home Economics Education published in the early (1980-1983) and the late 1980s (1986-89). The researcher developed instrument used in this study consisted …

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A Study Of Metacognitive Skill As Influenced By Expressive Writing In College Introductory Algebra Classes., Naomi Barbara R Allen Jan 1991

A Study Of Metacognitive Skill As Influenced By Expressive Writing In College Introductory Algebra Classes., Naomi Barbara R Allen

LSU Historical Dissertations and Theses

The quality of mathematics education has become a major concern to mathematics educators. As a result, increased attention is being given to identifying the abilities that underlie competent performance. An outcome of this effort is an increasing belief that the development of metacognitive skills is an essential component of proficient mathematics performance. Writing, because it promotes reflective thinking, is believed to be the vehicle for this development. Writing in the mathematics classroom has previously received anecdotal support for its benefits to the learner and to the instructor, and limited quantitative benefits in problem-solving ability toward mathematics. This study examined the …

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Novel Alumina-Based Stationary Phases For Liquid Chromatography., Rene V. Arenas Jan 1991

Novel Alumina-Based Stationary Phases For Liquid Chromatography., Rene V. Arenas

LSU Historical Dissertations and Theses

The chromatographic properties of polymer-coated Unisphere aluminas for reversed-phase liquid chromatography were evaluated and compared to those of Si-C$\sb{18}$ phases. A greater difference in solvent strength between acetonitrile and methanol was observed for the Unisphere columns. Approximately equal methylene selectivity was also obtained for both stationary phase types. Evaluation of polar group selectivity showed the absence of hydroxyl group participation in solute retention for the aluminas. In terms of kinetic properties, acceptable reduced plate heights were obtained for the Unisphere columns at optimum velocities. However, the van Deemter curves for the aluminas showed a more rapid loss in efficiency with …

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The Immunoepidemiology Of Lung Cancer And Smoking: Lymphocyte Subsets., Joan Peters Blackmon Jan 1991

The Immunoepidemiology Of Lung Cancer And Smoking: Lymphocyte Subsets., Joan Peters Blackmon

LSU Historical Dissertations and Theses

The relative proportions of lymphocyte subsets in the peripheral blood constitute a measure of immune status. Results of research to establish whether or not individuals with lung cancer differ in their lymphocyte composition have conflicted due to failure to control for factors other than cancer that affect lymphocyte balance. This study was designed to quantitatively estimate the effects of variables such as disease (lung cancer), smoking, age, circadian and circannual rhythms, and dietary factors on lymphocyte subsets. Questionnaires were given to 64 white males with primary lung cancer and 219 cancer-free white males and a blood sample was obtained. Lymphocytes …

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A Multidimensional Career Commitment Measure (Mccm) Gauging Motivation To Work In One's Career Field., Kerry D. Carson Jan 1991

A Multidimensional Career Commitment Measure (Mccm) Gauging Motivation To Work In One's Career Field., Kerry D. Carson

LSU Historical Dissertations and Theses

Past career commitment measures have been redundant with other work commitment measures or have been narrowly defined as professionalism. Blau's (1985) definition of career commitment as one's attitude towards one's vocation seems to most closely represent this construct. However, his measure has several problems including construct overlap with career withdrawal intentions. Because of a developmental lag in the career focus domain due to lack of established measures, the reported research constructed and tested a theoretically based measure of career commitment. Development of a new career commitment measure was based on London's (1983) theory of career motivation consisting of three theoretical …

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An Adaptive Differencing Scheme For Elliptic Flows., Therese Estelle Rhodes Jan 1991

An Adaptive Differencing Scheme For Elliptic Flows., Therese Estelle Rhodes

LSU Historical Dissertations and Theses

This thesis deals with the formulation of a computationally efficient multiple-grid adaptive differencing (MAD) scheme for two-dimensional elliptic flow and heat transfer problems. This algorithm equidistributes a measure of the error by using higher order differencing schemes locally in adaptively determined high error-estimate regions. The third-order accurate QUICK scheme is used in regions of high error estimate which are dynamically flagged on the basis of a preliminary first order upwind solution. Boundary conditions for the flagged regions are taken from the preliminary upwind solution. Multigrid type calculations are performed. Three multiple-grid schemes are developed. In the first scheme, MAD1-WFDS, the …

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Behavioral Characteristics Of Damaged Steel Repaired By Heat Straightening., Paul Franklin Robinson Jan 1991

Behavioral Characteristics Of Damaged Steel Repaired By Heat Straightening., Paul Franklin Robinson

LSU Historical Dissertations and Theses

A comprehensive set of experiments is conducted on damaged steel plates and rolled shapes which have been subjected to heat straightening. Effects of the repair process on the tensile properties and residual stresses in the repaired steel are experimentally determined for the first time. Based on the results, rational decisions can be made concerning the use of heat straightening and its limitations. For the first time in a laboratory setting, heavily damaged plate elements and rolled shapes are repetitively subjected to vee heat applications to produce desired straightening. Movements resulting from each heat application are recorded and provide an adequate …

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Student Thought Processes And Quality Of Practice During Motor Skill Instruction., Melinda Ann Solmon Jan 1991

Student Thought Processes And Quality Of Practice During Motor Skill Instruction., Melinda Ann Solmon

LSU Historical Dissertations and Theses

The focus of this study was the examination of relationships between initial skill level, observer estimates of attention, student thoughts, quality of practice, and achievement during motor skill instruction. Specifically, the following questions were addressed: (1) What is the relationship between observed student behavior to quality of practice and achievement? (2) What is the relationship between student reports of their cognitive processes to quality of practice and achievement? (3) Does written self-report data produce the same information as data collected during stimulated recall interviews? and (4) Are student self-report data about attention consistent with observer estimates of overt student behavior? …

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An Analysis Of Sequential Patterns Of Instruction In Piano Lessons., Donald Ray Speer Jan 1991

An Analysis Of Sequential Patterns Of Instruction In Piano Lessons., Donald Ray Speer

LSU Historical Dissertations and Theses

The purpose of this study was to investigate verbal behaviors of independent piano teachers in the setting of the private piano lesson. Twenty-five piano teachers from southeast Louisiana participated in the study. Teachers recorded the individual lessons of two students on audiotape. Verbatim transcripts were developed from a total of 47 recorded lessons. Transcripts were coded, identifying verbal behaviors with regard to components of sequential patterns established by Yarbrough and Price (1981, 1989). Verbal behaviors were analyzed for time spent in the categories of teacher presentation, student participation, and teacher reinforcement. Frequencies were obtained for the number of complete/correct, complete/incorrect, …

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Metabolic Pathways Associated With Glycogen Storage In Chronically Active And Normal Skeletal Muscle., Robert James Talmadge Jan 1991

Metabolic Pathways Associated With Glycogen Storage In Chronically Active And Normal Skeletal Muscle., Robert James Talmadge

LSU Historical Dissertations and Theses

To determine the pathways used for glycogen synthesis in chronically active and normal muscle, muscles from C57B1/6J dy$\sp{\rm 2J}/$dy$\sp{\rm 2J}$(dy$\sp{\rm 2J}$) mice were evaluated biochemically and histochemically. The gastrocnemius muscle (GAST) of the dy$\sp{\rm 2J}$ mouse, a chronically active muscle, contained twice as much glycogen and lactate as control GAST. The dy$\sp{\rm 2J}$ triceps muscle (TRI), which is not chronically active, showed no elevation in glycogen content. An intraperitoneal injection of $\sp{14}$C-lactate resulted in increased incorporation of $\sp{14}$C into glycogen by dy$\sp{\rm 2J}$ compared to control GAST. Both normal and dy$\sp{\rm 2J}$ GAST incorporated $\sp{14}$C into glycogen in an in …

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Studies In Fructofuranose Chemistry., Ronald Joseph Voll Jan 1991

Studies In Fructofuranose Chemistry., Ronald Joseph Voll

LSU Historical Dissertations and Theses

The glycolytic pathway enzyme phosphofructokinase (E.C. 2.7.1.11) has as its substrate and activator two different carbohydrate phosphate esters. Both are derivatives of D-fructose in the furanose ring form. This dissertation is divided into two chapters: the first deals with the conformational specificity of the active site and the second the structure of the activator. The compound 2,5-anhydro-3,4-O-(1,2-ethanediyl)-D-mannitol was designed to test the active site. The synthesis utilized a phase-transfer cyclo-dialkylation of a vicinal diol to yield a trans-fused 2,5,8-trioxabicyclo (4.3.0) nonane system. The final product was shown to exist in a locked conformation in solution by temperature dependent n.m.r. experiments …

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