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Articles 151 - 180 of 7215
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Transformation Efficiency And Gfp Expression In E. Coli, Jessica H. Hobbs
Transformation Efficiency And Gfp Expression In E. Coli, Jessica H. Hobbs
Honors Theses
Genetic transformation of bacteria is a foundational technique in molecular biology and biotechnology, enabling the introduction and expression of foreign genes for research, industrial, and educational applications. This thesis investigates the transformation efficiency and green fluorescent protein (GFP) expression in Escherichia coli using the pGLO plasmid system, with the primary objective of supporting the development of a graduate-level laboratory course focused on bioprocess engineering and molecular biotechnology. The pGLO plasmid contains a GFP reporter gene regulated by an arabinose-inducible promoter and an ampicillin resistance gene that allows for selective growth of transformed cells. Competent E. coli HB101 K-12 cells were …
Toward An Understanding Of Social Marketing’S Influence On Alcohol-Related Behaviors In College Students: Health Promotion Strategies For College Campuses, Lily K. Richards
Toward An Understanding Of Social Marketing’S Influence On Alcohol-Related Behaviors In College Students: Health Promotion Strategies For College Campuses, Lily K. Richards
Honors Theses
Alcohol use among college students remains a persistent public health concern, influenced by a complex interaction of social, environmental, and behavioral factors. This literature review examines the prevalence of alcohol consumption in college populations, the associated short-term and long-term health consequences, and the role of social norms, peer influence, and environmental context in shaping drinking behaviors. Research consistently demonstrates that alcohol use is deeply embedded within college culture, where normative misperceptions and peer dynamics contribute to increased consumption. (Perkins, 2002; Borsari & Carey, 2001; Hingson et al., 2017). In addition, environmental factors such as alcohol availability and targeted marketing further …
Salt-Dependent Purification Of Green Fluorescent Protein Via Hydrophobic Interaction Chromatography, Katie L. Collins
Salt-Dependent Purification Of Green Fluorescent Protein Via Hydrophobic Interaction Chromatography, Katie L. Collins
Honors Theses
Green fluorescent protein, GFP, is a widely used fluorescent reporter, and its purification provides a clear model for studying downstream bioprocessing. This thesis investigates how binding-buffer salt strength affects GFP retention and elution during hydrophobic interaction chromatography, HIC, by comparing three conditions (1.0×, 0.8×, and 0.6×). GFP-expressing E. coli were lysed and clarified, and the lysate was purified on HIC columns; collected fractions were evaluated qualitatively under UV illumination to track fluorescence distribution. Results showed a salt-dependent trend in which higher salt improved retention and shifted fluorescence toward later elution fractions, while reduced salt weakened hydrophobic interactions and increased earlier …
Community Resilience And Response To Salinity Gradients In Streams Flowing Into Biscayne Bay, Florida, Sebastian M. Jimenez
Community Resilience And Response To Salinity Gradients In Streams Flowing Into Biscayne Bay, Florida, Sebastian M. Jimenez
Honors Theses
Salinity is a shaping force of aquatic biodiversity, and ongoing climate change and unsustainable water-management practices are causing salinity-induced stress across coastal ecosystems. Meiofauna are small (45-1000 μm), taxonomically diverse aquatic invertebrates that are vital to ecosystems and highly responsive to environmental disturbances, making them ideal for assessing the effects of changing salinities on community structure. This study investigated how meiofaunal biodiversity (ASV richness, Faith’s phylogenetic diversity, and community composition) responded to natural salinity gradients along streams flowing into Biscayne Bay, South Florida, which is a region particularly vulnerable to salinization due to sea-level rise, altered hydrology, and watershed modification. …
The Effects Of The South Chattanooga Mentorship Program On Elementary School Children, Ana G. Morris
The Effects Of The South Chattanooga Mentorship Program On Elementary School Children, Ana G. Morris
Honors Theses
This research study examines the impact of mentorship within the South Chattanooga Community Center’s after-school program, with a focus on elementary-aged students. The purpose of this study is to evaluate how one-on-one mentorship affects students’ academic development, behavior, and social-emotional learning. The program operates as a cost-free, five-day-a-week initiative designed to provide a safe and supportive environment for children who may lack consistent guidance outside of school. Using observational methods and direct interaction with students (quantitative and qualitative), this study examines mentorship influences key skills, including regulation, communication, relationship-building, and literacy abilities. It suggests that students who participate in the …
Bottles, Butterflies, And Biomass: Recycled Glass Sand, Microbial Additions, And Herbivory Effects On Milkweed Growth, Kealey Skinner
Bottles, Butterflies, And Biomass: Recycled Glass Sand, Microbial Additions, And Herbivory Effects On Milkweed Growth, Kealey Skinner
Honors Theses
Plant-soil-microbe interactions are integral components of ecological networks and drive ecosystem function from the bottom-up. Whereas herbivores regulate ecosystem function from the top-down, having opposing and potentially complementary effects on ecosystem function. My thesis sought to understand how below-ground microbial communities contribute to herbivore preference and plant biomass across varied soil substrates. My thesis asked: how do substrate and soil microbial communities influence herbivore preference and plant biomass accumulation on swamp milkweed (Asclepias incarnata)? To address this question, seedlings of A. incarnata were sown in 2.5 cm wide × 10 cm deep pots filled with either natural beach sand or …
Comparative Investigation Of Bacterial Adhesion On Medical Device Thermoplastics, Dev V. Mehta
Comparative Investigation Of Bacterial Adhesion On Medical Device Thermoplastics, Dev V. Mehta
Honors Theses
This project will focus on determining if differences in bacterial cell wall composition influences adhesiveness to thermoplastic materials used to construct patient orthotic splints. Bacteria with decreased adhesion are unable to remain bonded to orthotic splints, increasing its ability to transfer to a patient’s wound, leading to an infection. Antibiotic resistant bacteria used in this investigation included gram-positive cocci vancomycin-resistant (VRE) Enterococcus faecalis, methicillin-resistant Staphylococcus aureus (MRSA), and gram-negative rods extended-spectrum beta-lactamase (ESBL) Escherichia coli and Pseudomonas aeruginosa. Each test bacterium is a major pathogen causing healthcare-associated infections (HAIs). Saline, artificial perspiration, and ascites fluids were used to determine the …
Search For Slow-Moving Magnetic Monopoles With An Improved High-Energy Event Removal Algorithm, Reeshi N. Gihosal
Search For Slow-Moving Magnetic Monopoles With An Improved High-Energy Event Removal Algorithm, Reeshi N. Gihosal
Honors Theses
Fermilab’s NOvA (NuMI Off-axis 𝑣𝑒 Appearance) experiment focuses on understanding the behavior of neutrinos and how they affect the cosmos. A sub-focus of the NOvA experiment is the search for magnetic monopoles. These elusive particles have not yet been observed in nature, leaving their behavior to be mysterious. The Far Detector, located in Ash River, MN, is integral in the search for these particles. This project is on simulated magnetic monopoles with speeds thousandths the speed of light, with focus on the role of slicing algorithms in event reconstruction using the NOvA experiment’s reconstruction algorithm. Using sample data, analysis occurred …
Determinants Of Hypertension Awareness And Control In Kenya: A Secondary Analysis Of National Survey Data, Alexander K. Davis
Determinants Of Hypertension Awareness And Control In Kenya: A Secondary Analysis Of National Survey Data, Alexander K. Davis
Honors Theses
In low- and middle-income countries (LMICs), cardiovascular diseases (CVDs) are prevalent and pose a major concern as the current healthcare systems are oriented primarily towards communicable diseases. The largest contributor to CVDs, hypertension, is a significant issue in Kenya, a model LMIC. Based on previous studies in this lab, high rates of hypertension are present in the region of Kasigau, Kenya. Kasigau is a small region within Taita-Taveta County, Kenya. Traditional risk factors are not necessarily predictive of hypertension in all regions of Kenya. This study aims to address the lack of tested risk factors for hypertension in Kenya and …
Utilizing Brain Computer Interfaces That Interact With A Virtual Keyboard, Skye Lilienthal
Utilizing Brain Computer Interfaces That Interact With A Virtual Keyboard, Skye Lilienthal
Honors Theses
A brain-computer interface (BCI) can allow someone to utilize electrical signals in their brain to complete tasks using a computer. BCIs can help people take advantage of technology to type without the need for a traditional keyboard setup. This paper used the OpenBCI Mark IV to test the effectiveness of non-invasive BCIs with dry electrodes within the OpenViBE P300 Speller. This paper shows how to use the P300 speller through a setup pipeline. Results indicate that electrode placement affects P300 accuracy and that areas related to visual processing improve accuracy, suggesting that P300 signals can be detected within OpenBCI Mark …
Finnegan's Got A Secret: An Exercise In Playwriting, Ari Theodore
Finnegan's Got A Secret: An Exercise In Playwriting, Ari Theodore
Honors Theses
No abstract provided.
The Personality Of Place: Physical Geography And The Shaping Of The Nineteenth-Century German Immigrant Ethnic Identity, Abigail Ward
The Personality Of Place: Physical Geography And The Shaping Of The Nineteenth-Century German Immigrant Ethnic Identity, Abigail Ward
Honors Theses
In the nineteenth century alone, approximately five million German-speaking immigrants arrived in America. However, the motivations of these individuals for moving to America, far from identical, were nearly as numerous as the individuals themselves. The German ethnic identity in America was not uniform, but the expression of many factors having to do with both the Vaterland and the new land. These factors ranged from the timing of emigration, which was largely influence by religious, political, and economic upheaval in Europe, to each German’s system of values, which dictated the kind of future he saw for himself and therefore where he …
Choosing Death: The Moral Legitimacy Of Voluntary Active Euthanasia, Emma Rougeou
Choosing Death: The Moral Legitimacy Of Voluntary Active Euthanasia, Emma Rougeou
Honors Theses
During the last three decades, the issue of physician assisted suicide has captivated the interest of private citizens and state legislatures. Thinking about the matter of end-of-life concerns, particularly surrounding euthanasian (practices that hasten the deaths of suffering terminally ill people) have shifted. Views of private citizens and state-level legislation reflects a “slow shift to more permissive attitudes” (Cohen et al., 2012). Meanwhile, as recently as March 2026, state legislators in conjunction with the NY State Governor, passed a bill legalizing medical aid in dying for terminally ill patients. This thesis is intended to thoughtfully contribute to meaningful discourse on …
Urea-Based Boronium Salts As Antimicrobial Agents, Gabriel Allen Merchant
Urea-Based Boronium Salts As Antimicrobial Agents, Gabriel Allen Merchant
Honors Theses
Quaternary ammonium compounds (QACs) are widely used in disinfectants due to their ability to inhibit the growth of and eliminate microbes. The potency, broad-spectrum activity, and low cost of QACs have made them a cornerstone of modem hygiene. In recent years, QACs have garnered many health and safety concerns due to their overuse and subsequent accumulation in the environment. The increased use of QACs has further led to an increase in antimicrobial-resistant bacteria, making the synthesis of novel antibacterial compounds vital to combat this growing threat. Recent advances in boronium salts offer a unique alternative to address these adverse effects. …
Spectral Characterization Of Colorectal Cancer Using Excitation-Scanning Hyperspectral Imaging, Natalie Hadad
Spectral Characterization Of Colorectal Cancer Using Excitation-Scanning Hyperspectral Imaging, Natalie Hadad
Honors Theses
Colorectal cancer is one of the most common and deadly cancers worldwide, with a lifetime risk of approximately one in twenty-three for men and one in twenty-five for women. Although early detection significantly improves survival, current diagnostic approaches such as tissue biopsy are invasive, reliant on subjective interpretation, have extended turnaround times, and risk missing flat or depressed lesions that are more difficult to detect than raised polyps. These limitations highlight the need for objective, minimally invasive technologies capable of detecting early tissue changes. This study investigates excitation-scanning hyperspectral imaging (Ex-HSI) as a label-free optical approach for identifying colorectal cancer–associated …
Developing A Protocol For Non-Cellular Transplantation Of Donor Mitochondria Into Recipient Cells, Andrew Parton
Developing A Protocol For Non-Cellular Transplantation Of Donor Mitochondria Into Recipient Cells, Andrew Parton
Honors Theses
Tumor innervation has emerged as a critical feature of cancer progression, with increased nerve density correlating with enhanced aggressiveness, metastatic dissemination, and poor clinical outcome. However, the functional contribution of neurons to tumor biology remains incompletely defined. We recently identified the intercellular transfer of mitochondria from neurons to cancer cells as a mechanism that promotes tumor progression. We therefore hypothesized that disruption of this transfer could attenuate tumor aggressivity. To directly investigate this process, we developed an approach to isolate mitochondria from donor cells and transplant them into recipient cancer cells independently of canonical cell-cell interactions. Transferred mitochondria were tracked …
Red 40 Riot: An Interdisciplinary Perspective On The Fight For Food Additive Regulation, Margaret Q. Gray
Red 40 Riot: An Interdisciplinary Perspective On The Fight For Food Additive Regulation, Margaret Q. Gray
Honors Theses
This paper explores the chemical, business, and governmental influences that impact the perception and future of Red 40. This inquiry was inspired by the increasing trends towards health-conscious consumer preferences and the 2025 Red 3 ban. This thesis examines foundational research, modern sources, and public-facing articles to create a comprehensive understanding of both sides of the argument in support of and against regulation. The paper concludes that a three-pronged approach would be ideal to target each key perspective included: chemistry, business, government. The approaches include confirming chemical research method validity, encouraging governmental regulation, and corporate accountability and enforcement. The paper …
The Oncoming Light: An Experiential Approach To Psychological Exposition Through Dialogue-Driven Narrative, Benjamin G. Dewitt
The Oncoming Light: An Experiential Approach To Psychological Exposition Through Dialogue-Driven Narrative, Benjamin G. Dewitt
Honors Theses
Narrative is a lens through which we understand ourselves, our histories, our futures, and our ties to those around us. Narrative form has undergone vast transformations over time, and in the case of “postmodern” literature, pushes the boundaries of form into the experimental, questioning the truth of knowledge itself. While these experimental works can give rise to uniquely powerful emotional experiences, some components of narrative, when abandoned, may only hinder the immersion of the reader. This thesis evaluates several experimental, dialogue-driven narrative works against these components: linguistic, literal, emotional, and intellectual significance, in a hierarchy of importance as proposed by …
Synthesis Of Novel 1,2,3-Triarylnaphthalenes Via Intermolecular Dehydro-Diels-Alder Reaction, Ethan C. Dailey
Synthesis Of Novel 1,2,3-Triarylnaphthalenes Via Intermolecular Dehydro-Diels-Alder Reaction, Ethan C. Dailey
Honors Theses
The photo-dehydro-Diels–Alder (PDDA) reaction is a useful method for the construction of substituted naphthalene derivatives from acyclic precursors. While intramolecular variants of this transformation are well established, intermolecular PDDA reactions remain rare due to challenges associated with controlling reactivity under photochemical conditions. Previous studies suggest that electron-withdrawing substituents can promote intermolecular cycloaddition by increasing alkyne polarization and stabilizing reactive intermediates. In this work, the effect of enhanced electron-withdrawing character on intermolecular PDDA reactivity was investigated through the incorporation of a 2,3,5,6-tetrafluoropyridinyl substituent. Corresponding alkyne substrates were synthesized and subjected to photochemical conditions, resulting in the formation of 1,2,3-triarylnaphthalene products. The …
The Privacy Paradox: How Does Concern About Privacy Impact Actions In The Digital Age?, Julia Laduke
The Privacy Paradox: How Does Concern About Privacy Impact Actions In The Digital Age?, Julia Laduke
Honors Theses
The privacy paradox occurs when people claim to care about their digital privacy, but do not take actions to keep their data from being spread across the internet. This study examines college students, being primarily Generation Z, and their concerns and actions regarding digital privacy and security. A survey of 15 college students, 7 in humanities and 8 in STEM, was used to analyze their thoughts and concerns about their digital privacy. This survey also asked whether they took actions concerning their privacy, and if so, what tools they used to protect it. The results show that about 50% of …
Calibrating Human Trust In The Age Of Generative Ai: An Examination Of Ethical And Social Challenges, Abigail M. Mondido
Calibrating Human Trust In The Age Of Generative Ai: An Examination Of Ethical And Social Challenges, Abigail M. Mondido
Honors Theses
Generative AI (GenAI), a set of AI technologies with the ability to generate original, human-like outputs, is beginning to transform the way that information is distributed, composed, published, obtained, analyzed, and consumed. GenAI has seen massive adoption by internet users, businesses, and organizations in recent years despite the persistence of major ethical concerns and social implications. In particular, existing research has identified multiple critical trust-related issues associated with AI in general, including widespread mistrust and distrust, overreliance on AI, and a lack of trustworthiness of AI. There remains a need for a broader understanding of these issues as they relate …
Skill Growth And Development In An Honors Experiential Learning Course, Julia D. Lyashevskiy
Skill Growth And Development In An Honors Experiential Learning Course, Julia D. Lyashevskiy
Honors Theses
Problem-solving skills are crucial for students in both schoolwork and the workforce as they help individuals and groups work through obstacles and come up with solutions to a problem. To help prepare students, the Innovation in Honors Lab at the University of Tennessee at Chattanooga is a hands-on, interdisciplinary course that provides an experiential learning opportunity to practice skills while working directly with community partners to find and solve a local issue. This research tracks the development of six specific skills that students use throughout the class. The skills included communication, creative thinking, decision making, problem solving, conflict resolution, and …
The Effect Of Chronic Aerobic Exercise On Working Memory: A Study On Young Adults, Alexandria J. Morris
The Effect Of Chronic Aerobic Exercise On Working Memory: A Study On Young Adults, Alexandria J. Morris
Honors Theses
Physical activity is essential for optimal health. Cognitive function is a major part of that, and can be evaluated through working memory (WM). This thesis examines the impact of physical activity on WM in young adult college students. It reviews existing research on exercise and WM, highlights gaps in the literature focused on this demographic, and explores the importance of chronic and moderate to high-intensity exercise to WM. There is existing research that examines the correlation of WM and physical activity for both acute and chronic exercise, as well as for children, adults, and older adults. Acute aerobic exercise shows …
Post-Dobbs: Observed Maternal And Infant Health Outcomes, Erin P. Yenawine
Post-Dobbs: Observed Maternal And Infant Health Outcomes, Erin P. Yenawine
Honors Theses
This research investigates how the variance in abortion legislation and access across the United States has impacted infant and maternal health. This builds upon previous research identifying a relationship between abortion policy and quantity of births, (Myers et al., 2025; Bell et al., 2025) abortion policy and infant and maternal health pre-Dobbs (Gardner, 2024), and an emerging body examining the health outcomes post-Dobbs for mothers and infants (Gemmill et al., 2025; Gressler & Lewis, 2025). Scholars indicate that primary contributors to increases in infant mortality may be the continuation of pregnancies with congenital abnormalities and compounded barriers of access to …
“Who Are We?”: Exploring Adolescent Identity And Community Through Collaborative Mural Art, Rachel E. Stacy
“Who Are We?”: Exploring Adolescent Identity And Community Through Collaborative Mural Art, Rachel E. Stacy
Honors Theses
This thesis explores how participation in a high school mural club influences students’ sense of personal identity and community. By creating and leading a mural club, I guided students in designing and painting murals around their school and extending this work into the local community, all focused on how students defined themselves within the context of the school’s motto: “A Star is Rising”. I used qualitative methods including surveys, observational notes, artist statements, and visual documentation to examine and analyze how students experienced identity and collaboration throughout the process. The findings show that the creation of these murals provided students …
Creative Nonfiction Essays, Colby S. Langley
Creative Nonfiction Essays, Colby S. Langley
Honors Theses
To write about the present with any authority requires a keen eye for the past. To write from our lives into meaningful creative nonfiction requires a firm understanding that experience is the composite of life experience blending into the current moment. The writing is then drawn irreversibly towards emotion: indulgent and oppressive. The extremity of emotion dictates the difficulty of transcribing the experience onto the page, and violence is a source of both extreme emotion and experience. It is important to note that I am primarily considering personal violence for this essay, while societal and institutional violence have their own …
A Narrative Of The Plague Doctor And How Its Historical Context Translates To The Modern View Of The Plague Doctor, Callie P. Johnston
A Narrative Of The Plague Doctor And How Its Historical Context Translates To The Modern View Of The Plague Doctor, Callie P. Johnston
Honors Theses
The Plague Doctor was once a historical figure who practiced medicine primarily on those infected with the bubonic plague. The plague doctor, as a historical figure and physician, has been transformed in modern culture and media. Originally perceived as a harbinger of both hope and death, the Plague Doctor has undergone a historical transformation from a masked physician to a more stylized character that appears in modern media, digital art, toys, costumes, video games, and popular culture. This thesis examines the evolution of the Plague Doctor from its historical role as a plague physician to its current iconography as a …
Evaluating The Effects Of Urbanization On Meiofauna Biodiversity In Chattanooga’S Lentic Ecosystems Through Gis Applications, Keyle M. Bryant
Evaluating The Effects Of Urbanization On Meiofauna Biodiversity In Chattanooga’S Lentic Ecosystems Through Gis Applications, Keyle M. Bryant
Honors Theses
Urbanization is a leading driver of biodiversity loss in freshwater ecosystems, yet its effects on meiofauna—microscopic invertebrates that serve as key bioindicators of ecosystem health—remain poorly understood in lentic environments. This study evaluated the effects of urbanization on meiofaunal biodiversity in ten lentic waterbodies across Hamilton County, Chattanooga, Tennessee, by integrating environmental DNA (eDNA) metabarcoding, in-situ abiotic measurements, and Geographic Information Systems (GIS)-based land use analysis. Water samples were collected between January and April 2023 and processed using 18S rRNA amplicon sequencing to assess biodiversity as richness (amplicon sequence variants or ASVs, phylogenetic diversity or PD, and community composition). Land …
How Do Community Gardens Contribute To A Thriving National Park City: A Case Study Of Chattanooga, Tennessee, Ansley B. Barry
How Do Community Gardens Contribute To A Thriving National Park City: A Case Study Of Chattanooga, Tennessee, Ansley B. Barry
Honors Theses
On April 3, 2025, Chattanooga, Tennessee became the first National Park City (NPC) in North America. With a long, industrial history, Chattanooga has made quite the pivot from being widely renowned for its low air quality and particulate pollution to being recognized as the Best Outdoor City in the US by Outdoor magazine. The goals emphasized by the National Park City campaign are creating a city-wide common interest of community-led sustainability efforts. While the initiative highlights many important initiatives to make the city more sustainable and resilient, community gardens are noticeably left out of the conversation. This paper argues that …
Dissociation, Anxiety, And The Vestibular System: Understanding The Impacts Of Anxiety, Panic, And Dissociation On The Vestibular System, Julian Achata
Honors Theses
Previous research indicates that vestibular dysfunction is associated with symptoms of anxiety, panic, and dissociation. The present study will investigate anxiety, panic, and dissociation symptoms and identify the connections between reported symptom severity and vestibular functionality. This study will focus on three different hypotheses: 1) higher levels of dissociation will correlate with increased vestibular disruption; 2) higher levels of anxiety will correlate with increased vestibular disruption; 3) higher levels of panic will correlate with increased vestibular disruption. Six virtually administered self-report measurements were used to identify the variables: Beck Anxiety Inventory (BAI), Depersonalization Derealization Inventory (DDI), Dissociation Experiences Scale-II (DES-II), …