Digital Commons Network^™

The Impact Of Cattle Grazing On Water Quality In Stoner Creek, Bourbon County, Ky., Logan Stigall

Honors Theses

This study aims to identify the impact of cattle grazing on the water quality of Stoner Creek, located in North Middletown, KY. Cattle grazing can impact water quality in numerous ways. In this study, water quality was examined using the following parameters: water temperature (°C), pH (SU), dissolved oxygen (mg/L), conductivity (μS/cm), and Escherichia coli (E. coli) colonies per 100 mL of water. Results show that Site 3 (Cattle access point) had the greatest average conductivity at 465 μS/cm. Sites 3 and 4 showed the highest turbidity. There was a strong positive correlation (r²=0.32, p=0.02) between conductivity and …

The Nil Ripple Effect: How Name, Image, And Likeness Reshapes Student-Athletes, Mental Health, And Fan Perception, Jaleah Walker

Honors Theses

The NIL Ripple Effect: How Name, Image, and Likeness Reshapes Student-Athletes, Mental Health, and Fan Perception examines the shifting dynamics of college athletics and how these changes affect student-athletes, fans, and institutions. The study explores the consequences of Name, Image, and Likeness (NIL) deals and its effects on student-athletes' mental health and fans’ perception. Through interviews with athletes and analysis of current policies, this research highlights the mental, financial, and competitive pressures that NIL places on student-athletes. While NIL provides financial opportunities, it also demands brand management, public visibility, and performance consistency, often leading to heightened stress. Additionally, this study …

Developing Novel Radical Protein Footprinting Methods For Application In Living Systems, Lyle Tobin

Honors Theses

Radical protein footprinting (RPF) is a family of structural biology techniques that generate radicals in situ to label solvent-accessible amino acid residues. Coupled with liquid chromatography/mass spectrometry, RPF enables the characterization of protein higher order structure and sites of protein-protein and protein-ligand interactions. While traditionally performed in vitro, recent advancements have extended RPF to ex vivo and in vivo applications, including intact cells, nematodes, and 3D cell cultures. RPF in living systems allows the structural analysis of proteins and protein complexes in their native environment, preserving physiological interactions, dynamic conformational changes, and structural contexts that might otherwise be lost …

Genomic Characterization Of Novel Bacteriophage: Bellis, Emmanauel Z. Gibbs

Honors Theses

Phages are the most abundant life-forms on the planet. Due to their unique ability to infect and lyse specific bacterial strains, phages have been proposed as a potential therapeutic agent to fight antibiotic resistance bacterial infections. Consequently, more research is required to further our understanding of phages. We were provided with an isolated, novel Mycobacterium bacteriophage: Bellis. Utilizing a bioinformatic approach, we successfully annotated Bellis’ genome using a variety of computer programs like GeneMark, Glimmer, HHpred, and PECAAN. We discovered that Bellis has 122 genes, including a RecB-like endonuclease and an AAA-ATPase, as well as 10 tRNAs. Through the genomic …

Genome Annotation Of Stargaze, Madison R. Murray

Honors Theses

Bacteriophages are viruses which solely infect bacteria. They are one of the most abundant and diverse biological entities, yet there is a limited amount of information on these viruses, including their gene functions. Several previously unknown phages have been discovered including Stargaze, whose bacterial host is Mycobacterium Smegmatis. Characterization of phage genes and their functions deepen our understanding of phages and potentially contribute to the broader biomedical effort associated with phages ability to combat bacterial infections. Stargaze is a G5 subcluster phage and it only has one other related phage in its cluster, that phage is from Madagascar. Using a …

Microplastics In Brachyurans: Comparing Contents Of The Atlantic Ghost Crab, Ocypode Quadrata, And Their Surrounding Sediments, Michael See

Honors Theses

The emergence of microplastics as a threat to physiological and environmental health has led to an increase in studies in recent years that aim to identify and quantify potential risks to marine ecosystems. Ocypode quadrata is a species that is a critical component of coastal ecology, leading this study to collect and analyze specimens regarding microplastics and their presence as a pollutant to the marine environment. Samples were collected from beaches along the Grand Strand of South Carolina. Microplastic density and diversity were recorded from investigating fecal pellets from the captive crab specimens to examine microplastic abundance. In addition to …

Institutional Connectedness And Social Support Among College Students: Links To Campus Involvement, Carissa Strum

Honors Theses

Background. College is a pivotal period in the lives of students, and an important aspect of the experience is involvement within the campus. College student wellness is also a growing area of interest in higher education, with increased attention on ways to help students thrive. This study explores which aspects of campus involvement contribute to students’ feelings of connectedness and social support. This study aims to 1) assess the association between campus involvement and social support among college students, 2) assess the association between campus involvement and institutional connectedness among college students, and 3) examine whether the association between campus …

Effects Of Acute Exercise On Cognition: Consideration Of Potential Moderators, Lucy C. Wilson, Elizabeth Carwile, Mara Liston, Elizabeth Gaines, Mckenzie Cotten, Mary Griffin

Honors Theses

The present thesis aims to determine the effects, if any, of six potential moderators on the effects of acute moderate-intensity exercise on cognitive functioning. The modes of exercise undertaken by the participants included treadmill (Experiment 1) and stationary bike (Experiment 2) exercise. Participants in each experiment either exercised or rested for twenty minutes and then participated in either a memory recall task or cognitive functioning (“Stroop”) task. The bouts of either exercise or rest on the stationary bike were followed by a rest period during which the participant would be exposed to either standard air oxygen levels (normoxia) or hypoxic …

A Search For Transcriptional Cofactors Of Gcm/Repo Involved In Determining Glia Cell Fate In Drosophila Melanogaster, Nivedha Kosalram

Honors Theses

In Drosophila melanogaster, the mechanism influencing the differentiation of glia and neurons is directed by a key transcription factor known as glial cells missing (gcm). gcm is a crucial regulator of cell fate decision, functioning as a binary switch to direct glial fate in gcm- positive cells and neuronal fate in gcm-negative cells (Jones, et.al, 1995; Hosoya, et.al, 1995; and Vincent, et.al, 1996). In addition to directing gliogenesis, gcm and its homolog gcm2 facilitate the determination of macrophages and tendon cells, highlighting that gcm acts with supplementary factors to induce various signaling pathways in different contexts (Jones, 2005; Johnson, et …

Economic Development In Cartersville, Ms: A Case Study Of A Non-Profit Organization's Community Development Projects, Mckenzie B. Perkins

Honors Theses

The Mississippi Delta Region has long faced significant economic, educational, and health challenges and a steady population decline since the 1940s. In response, community development projects have emerged as powerful tools to promote health, well-being, and social mobility. This thesis investigates the impact of community development initiatives in Cartersville, MS, focusing on the dental clinic, educational programs, and the economic development center established by a non-profit organization. Through the lens of community development theories, the research explores how community leadership, particularly partnerships between EverHope Ministries and Cartersville leaders, is pivotal in addressing health disparities in historically marginalized populations. By engaging …

The Impacts Of The Transfer Process Through The Eyes Of Stem Majors, Astrid Y. Lopez Matias

Honors Theses

This study explores the experiences of science, technology, engineering, and math (STEM) transfer students through the lens of Lily, a biology major who transferred from a large Midwestern university to a large Southeastern university. Employing a qualitative case study approach, data were collected via a semi-structured interview to delve into the complexities of her transfer journey. The analysis was guided by the Edalgo and High (2022) framework, which examines the interplay of personal, institutional, and social factors in shaping student experiences.

Lily's narrative highlights several challenges common among STEM transfer students, including unexpected personal and financial pressures, difficulties in navigating …

Microplastic Concentrations Within The Winyah Bay River Plume, Daniel C. Haughie

Honors Theses

In the last 20 years, microplastics have become a significant concern for ecosystem and public health. The buoyancy and method of introduction makes river plumes a likely repository for microplastic accumulation. A river plume is a bulge of fresher water that expands radially from the mouth of an estuary, with distinct lines called fronts at the leading edge of the plume. A front is a region of convergence between the fresher plume water and the saltier ocean water. The density differences between the two water masses may create an easily identifiable color and salinity gradient. These fronts often allow for …

Determination Of Accuracy In Forensic Phenotyping With Implementation Of Microspectrophotometry And Snp Testing, Kayla Schuler

Honors Theses

Forensic DNA phenotyping and single nucleotide polymorphisms (SNP) testing has been a recent tool practiced in the field of forensic science, especially for identification purposes. DNA phenotyping had drawn debate in terms of its legislation and use, as it is practiced more in private sectors compared to local, state and federal laboratories (Schneider et al., 2019). When looking at specific SNPs, some are better associated in accurately predicating phenotypes for hair and eye color than others. Microspectrophotometry was combined with SNP testing in this study to determine if there was an improved accuracy in determination of genetic hair color vs …

Elemental Humanity: A Hybrid Collection Of Creative Work & A Critical Introduction Exploring Relevant Genres & Themes, Haylee G. Morman

Honors Theses

This project is a creative and critical endeavor with two components: a creative portion and a critical introduction. The creative portion is a hybrid collection of poems and short prose spanning across genres and forms, linked by thematic consistencies, united by their emphasis on nature and family. Furthermore, the collection is rooted in my interest in magic realism and ekphrasis, which will be discussed in the critical introduction. The approximately 30 pages of original, creative writing reveals how delving into natural elements for inspiration spurred the creation of poems and stories that grapple with notions of familial connection, time, place, …

Investigating Preservice Elementary Teachers (Psts’) Views Of Nature Of Science (Vnos) And Science Attitudes Following Engagement In An Integrated Physics And Chemistry Content Course, Anna Stahurski

Honors Theses

Students’ classroom experiences affect their views of science, placing importance on teachers’ instructional approaches. Current reform suggests that inquiry-based science instruction supports positive views of science. However, students and teachers have been shown to hold developing views of nature of science (VNOS), or understanding what science is and how it progresses. Compounding matters, every student has their own personal science experiences that shape their science understandings and attitudes. This project investigates the attitudes and VNOS among preservice elementary teachers (PSTs) enrolled in an integrated physics and chemistry content course (SCI142). Two course sections were surveyed throughout one semester (n = …

Correlation Between Maximal Strength And Standing Power Position In Shot Put Throwers, Ryan E. Joule

Honors Theses

This study determined the correlation between maximal strength to performance in a standing power shot put throw. Collegiate athletes (N = 7, 1 female, 6 males) conducted a one-repetition maximum for the bench press, back squat, and hang power clean, and executed three shot put throws at two different time points (three months apart). For the first time point, there were positive, moderate correlations of shot put distance to bench press (r = 0.41) and back squat (r = 0.45). A positive, strong correlation between distance thrown to hang power clean (r = 0.66) was present. For the second time …

Genomic Analysis Of The Lyme Disease Vector Across Virginia’S Spatial Expansion Front, Amy Jablonski

Honors Theses

Lyme disease is the most common vector-borne disease in the United States and is expanding with rising incidence in Virginia . This study investigates populations of lxodes scapularis across Virginia using RAD-seq to analyze SNPs from ticks collected in seven Virginia counties and four U.S. states as references . Results show a clear genetic split between western and eastern Virginia ticks . These results follow previously noted patterns in regional differences in Lyme disease risk.

Design And Optimization Of Aquaculture Gear: Development Of A Cage-Integrated Reeling Mechanism For Oyster Farming, Felicity Bryant

Honors Theses

This project encompasses the design, fabrication, and testing of a cage-integrated reeling system aimed at reducing the physical labor required in oyster aquaculture at Bama Bay Oyster Farm, the last operational oyster farm in Mobile Bay. The proposed system includes two primary components: lightweight, modular oyster cages and a dual-winch reeling device for automated retrieval. Three cage prototypes—rectangular, oval, and hexagonal—were evaluated for structural performance and fabrication feasibility. The oval design was ultimately excluded due to practical fabrication limitations. The rectangular and hexagonal designs were fabricated using recycled materials and tested under the most extreme environmental conditions derived from years …

Exploring The Role Of The Id2 And Rab11fip3 Pathway In X-Linked Chronic Granulomatous Disease (Xcgd) Through Comparative Gene Expression Analysis, Kaili Coenen

Honors Theses

X-linked chronic granulomatous disease (XCGD) is a debilitating immunodeficiency disorder primarily affecting men. The disease results from mutations in the CYBB gene on the X chromosome, which encodes the GP91^PHOX protein responsible for electron transfer from NADPH to molecular oxygen. Despite the identification of the CYBB mutation, the pathological mechanism underlying the reduction in reactive oxygen species (ROS) generation in XCGD remains unclear. We hypothesized that comparative gene expression analysis, combined with pathway analysis, could identify genes and signaling pathways contributing to reduced reactive oxygen species (ROS) levels in XCGD. In this study, we analyzed gene expression data from four …

The Relationship Between Sense Of Agency And Perceived Stigma In Those With Cognitive Disabilities, Scarlet S. Pardue

Honors Theses

The focus population of this study is those with cognitive disabilities, which limit in some way a person’s ability to complete mental tasks, such as communication, planning, or remembering. The primary purpose is to focus on the participants' sense of agency and perceived stigma. The Sense of Agency Scale (SoA) measures a participant’s consciously perceived control over their mind, body, and the immediate environment. The Perceived Disability Social Stigma Scale (PDSS) measures to what degree participants believe that people with disabilities are stigmatized in their respective communities. Confirmatory research into these two variables offers an interesting insight into how the …

“How Close To The Edge We Are”: Ecologically Reading The Frontiers Of Joan Didion’S Early Nonfiction, Mia R. Speller

Honors Theses

Joan Didion’s work is heavily regional and rooted in place, whether that be the place about which she writes or, by contrast, the place she leaves in order to do her writing. This place-based context takes root in many American ideologies and mythos, like that of the frontier mentality and golden dream. For this reason, exploring Didion’s use of natural metaphor and description as she discusses various cultural phenomena is essential to understanding the layers of her social critiques of 1960’s and 1970’s American culture, especially as they reflect on the failures of the frontier ideology. Further, looking at her …

Nutrition, Sleep, And Training Habits Enhance Endurance Race Performance, Anna F. Breed

Honors Theses

In recent years, marathon and ultra-marathon participation has grown substantially. Past research has shown that a range of variables influence the performance of endurance athletes. This study included participants of the Forgotten Florida 50-mile and 100-mile race and the Chattanooga Erlanger Half-Marathon and Marathon. These athletes were questioned about their sleep, nutrition, and training habits implemented for the races they completed. The results show the significance of carbohydrate consumption throughout the race for energy maintenance and shortened race times. Also, it was found that hill repeats and tempo runs brought about more successful race times for the runners who implemented …

Synthesis Of Single Chain Polymer Nanoparticle Networks By Reinitiating Raft Polymerization, Dallas Donovan

Honors Theses

Hydrogel materials, which are polymer networks comprised mostly of water, hold significant promise for biomedical applications due to their compatibility with biological systems. Conventional hydrogel materials are often brittle and weak, but most applications in biomedical systems require materials to be strong and tough. The topology of a polymer network dictates its mechanical properties. An important strategy for designing tougher hydrogels is the release of stored length, such as from the unfolding of polyprotein cross-linkers. Inspired by the success of the strategy, we report progress towards a fully synthetic design using single chain nanoparticles (SCNPs) into a hydrogel. SCNPs, which …

Sexism In Syntax: Language And Its Relationship With Female Leadership, Kyle Rains

Honors Theses

Despite a global trend towards more equal gender representation, not every country has improved at the same pace or to the same level. This study investigates language as a potential tool for better understanding the differences between countries regarding the presence of female managers. By using language as a tool, I arrive at two primary results: (i) pronoun drop is significantly and inversely associated with the presence of female managers, but gendered language structures have little association, and (ii) a gender intensity index can produce misleading results. These results suggest that language structures, such as pronoun drop, which are associated …

Perceptions Of Power And Consent: The Role Of Gender, Race, And Sexual Orientation In Assessments Of Victimhood In High School Student-Teacher Relationships, India Campbell

Honors Theses

Sexual abuse, in all of its many forms, has been a controversial topic for centuries. This project looks at a specific subset of sexual abuse: high school student-teacher assaults. Victims of sexual abuse often face barriers to justice. This project addresses several demographic barriers. Research has shown that people of color and members of the queer community are typically reluctant to report due to police and societal perceptions of these groups. Teenagers face additional challenges, such as not know how their peers, parents, school, or community will react. Additionally, their abuser is an authority figure, meaning that these victims could …

Role Of Evolutionarily Conserved G4 Structures In Enhancer-Promotor Interactions, Shahem Alqudah

Honors Theses

Gene regulation is governed by complex networks of interactions between enhancers and promoters, regions of the genome that are typically distant from one another yet functionally linked through chromatin looping. Recent research has revealed the significant role of Gquadruplexes (G4s) in facilitating these interactions. This thesis explores the role of long Gquadruplex regions (LG4s) in enhancer–promoter communication, proposing that these regions mediate physical interactions between enhancers and their target promoters through direct G4:G4 interactions. Using a chromatin conformation capture (EQuIP-seq), electrophoretic mobility shift assays (EMSA), and luciferase reporter assays, the study demonstrates that LG4s may drive gene expression by promoting …

Biophysical Characterization Of Thermophilic Branched-Chain Amino Acid Periplasmic Binding Protein From Thermotoga Maritima, Sky Marsicano

Honors Theses

Members of the periplasmic binding protein superfamily are involved in the selective passage of ligands through bacterial cell membranes. The hyperthermophilic eubacterium Thermotoga maritima encodes a putative periplasmic branched chain amino acid binding protein (TM1135). The gene was cloned into pET21a(+), overexpressed in Escherichia coli, purified using Ni-NTA chromatography, and confirmed with SDS-PAGE and MALDI mass spectrometry. Biophysical characterization of the protein was performed with fluorescence spectroscopy, absorbance spectroscopy, and circular dichroism experiments to investigate structural stability and ligand binding ability. Using circular dichroism, we demonstrate that TM1135 binds to the branched chain amino acids isoleucine, leucine, and valine, and …

A Comparative Analysis Of Nf-Κb1 Gene Regulatory Sequence Methylation In Normotensive And Hypertensive Kenyans, Aaryan Barlas Piracha

Honors Theses

Accounting for the majority of deaths worldwide, non-communicable diseases (NCDs) present the greatest health challenge of the twenty-first century. Specifically, cardiovascular diseases (CVDs) exceed all other NCDs in annual deaths and especially affect low- and middle-income countries (LMICs). Hypertension, being the primary risk factor for CVD, affects over 75% of adults in LMICs due to inadequate health care and preventative measures. Additionally, epigenetic modifications of DNA are important mechanisms that regulate gene expression; DNA methylation, in particular, affects cytosine residues in cytosine-phosphate-guanine (CpG) islands on regulatory sequences. Previous research in our laboratory analyzed percent methylation at 8 different CpG islands …

Decoding Bacterial Resilience: Srnas And Sigma Factors In Stress Adaptation And Persistence Of Salmonella, Anita Nguyen

Honors Theses

Small RNAs (sRNAs) are noncoding RNA molecules in bacteria that function primarily to regulate gene expression, including those associated with stress. The Borchert Lab has identified 173 sRNAs and > 500 sRNAs that are responsive to desiccation and carbon starvation (c-starvation), respectively. Prokaryotic transcription involves an RNA Polymerase (RNAP) that transcribes DNA into RNA. RNAP involves sigma factors, which recognize promoters for the initial steps of RNA synthesis. The Borchert Lab has determined that there are significant overlaps between sRNAs expressed in cells subjected to both short-term durations and long-term durations of carbon starvation. Salmonella enterica serovars can have outbreaks associated …

Pseudomonas Aeruginosa Exoenzyme Y Enzymatic Activity Inhibits Exoenzyme S-Induced Caspase Activation But Not Cytotoxicity In Pulmonary Microvascular Endothelial Cells, Connor Holm

Honors Theses

Pseudomonas aeruginosa (P. aeruginosa) is the most common cause of ventilator-associated pneumonia in intensive care unit patients. P. aeruginosa utilizes a type III secretion system that injects exoenzymes U, S, T, and/or Y into the host cell cytosol. A majority of clinically isolated P. aeruginosa strains express ExoY often in combination with ExoU and ExoT, or ExoS and ExoT. Host signaling mechanisms in response to intoxication with individual exoenzymes have been extensively studied, yet little is known about host cell response to co-intoxication. In this study, pulmonary microvascular endothelial cells (PMVECs) were infected with P. aeruginosa strains expressing different combinations …