Digital Commons Network^™

Pseudomonas Aeruginosa Exoenzyme Y Enzymatic Activity Inhibits Exoenzyme S-Induced Caspase Activation But Not Cytotoxicity In Pulmonary Microvascular Endothelial Cells, Connor Holm

Honors Theses

Pseudomonas aeruginosa (P. aeruginosa) is the most common cause of ventilator-associated pneumonia in intensive care unit patients. P. aeruginosa utilizes a type III secretion system that injects exoenzymes U, S, T, and/or Y into the host cell cytosol. A majority of clinically isolated P. aeruginosa strains express ExoY often in combination with ExoU and ExoT, or ExoS and ExoT. Host signaling mechanisms in response to intoxication with individual exoenzymes have been extensively studied, yet little is known about host cell response to co-intoxication. In this study, pulmonary microvascular endothelial cells (PMVECs) were infected with P. aeruginosa strains expressing different combinations …

‭Prevalence Of Potentially Pathogenic Bacteria In Showers At A Long-Term Nursing Care Facility, Joseph Caleb Kulon

Honors Theses

Pathogenic bacteria on environmental surfaces in nursing homes may contribute to the‬ growing burden of healthcare-associated infections (HAIs), particularly as the U.S. population‬ ages and more individuals transition into long-term care. In this study, 70 swab samples were‬ collected from five community shower rooms at a nursing home over three sampling trips. Five‬ common shower surfaces were sampled: the floor drain, shower bench, faucet/valves, shower‬ curtain, and grab bars. Samples were processed in the UTC CIDC microbiology lab and‬ inoculated onto four selective and differential media: Hardy CHROM MRSA agar (for‬ methicillin-resistant‬‭ Staphylococcus aureus‬‭ [MRSA]‬‭ and‬‭ S. pseudintermedius‬‭ [SIM]), mannitol‬ …

Bacterial Contamination Of Wheelchairs In A Long-Term Rehabilitation Facility, Storm A. Welch

Honors Theses

The presence of bacterial contamination on wheelchair surfaces in long-term care facilities poses a significant risk to infection control and patient safety. This study aimed to assess the prevalence and diversity of bacterial species, including Staphylococcus aureus (SA), methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis (SE), Pseudomonas spp., and enteric bacteria on five key components of wheelchairs at a local long-term care facility. A total of 320 swabs were collected from wheelchair components, including handles for pushing, armrests, seats, wheel handles, and tires. Samples were analyzed using selective culture media, and bacterial species were identified based on colony morphology. The results …

Highly Pathogenic Avian Influenza Outbreak Preparedness In The United States: A Scoping Review, Emma J. Gurley

Honors Theses

In order to gauge preparedness for an outbreak of highly pathogenic avian influenza (H5N1) in the United States, a scoping review of the current literature was conducted. This process involved (1) identifying the research question, (2) identifying studies relevant to the topic, (3) selecting studies for inclusion (4) charting the data, and (5) analyzing and reporting the final data. Ultimately, seventeen studies were deemed appropriate for examination within this scoping review and several key themes were identified. The results emphasize the importance of adopting a One Health approach, the key role that vaccination may well play in preventing the spread …

Estimating Disease Transmissions With Assortative Mixing By Vaccination Status, Jacob D. Biesecker-Mast

Honors Theses

Many mathematical models of infectious disease assume the population is well-mixed, meaning every pair of individuals is equally likely to contact each other, potentially spreading the disease. In reality, populations are rarely well-mixed, and an important way in which they are not is assortative mixing, that is, when pairs of individuals who are similar are more likely to contact one another than pairs of individuals who are different. Failing to account for assortative mixing by vaccine status leads to biased estimates of important quantities that characterize disease transmission, including reproduction numbers. We expand on this by developing a model that …

Designing An Optimal Thermal Preference Arena For Southern Cricket Frog (Acris Gryllus) Juveniles, Eleanor E. Briggs, David M. Phillips Jr.

Honors Theses

Climate change has been altering ecosystems across the world at a substantial rate, disrupting the delicate ecophysiological relationships between ecosystems and the organisms that live in them. Ectotherms, such as amphibians, rely on external temperatures to behaviorally thermoregulate, making it especially imperative to study their thermal biology. Thermal preference, which assesses the environmental temperature an organism would seek out in the absence of external stimuli, provides valuable information about an amphibian’s thermal biology and can be used to predict the effects of climate change on a species or population. A commonly used method to measure thermal preference is an arena …

Early Detection Of Colon Cancer Through Colonoscopy: Analysis Of Tumor Size And Resection Length, Alyssa Haddock

Honors Theses

Colon cancer remains a leading cause of cancer-related deaths in the United States, with early detection critical for improved outcomes. Colonoscopy is a primary screening tool that can reduce incidence rates through the identification and removal of precancerous polyps. This study examines how undergoing a colonoscopy prior to diagnosis impacts two key surgical outcomes: tumor size and colon resection length. Data were collected from 70 de-identified patients who underwent colon resection for colon cancer between 2019 and 2024 at two regional hospitals in East Texas: Hunt Regional Medical Center and Mother Christus Frances Hospital. Using pathology reports, tumor size and …

A Kid's Guide To Infectious Disease, Abby Carroll

Honors Theses

A Kid’s Guide to Infectious Disease is an educational illustrated book intended for young pediatric patients, ages three to five. This project was inspired by my personal interests, involvement at the University of Mississippi, and experience as a medical assistant. Over the past couple of years, I have had the opportunity to work as a medical assistant, interacting with many pediatric patients and their families at HealthPointe Primary Care. As a pre-physician assistant student studying biology, I was inspired to create a resource for these kids by both my classes at the University of Mississippi and my passion for pediatric …

Natural Medicine Use In Costa Rica, Elizabeth Krol

Honors Theses

This study explores the role of natural medicine in Costa Rican culture, while focusing on its historical, cultural, and clinical significance. With a literature review, ethnobotanical research, and a study abroad experience, this paper highlights the integration of natural, herbal medicine into modern healthcare. Costa Rica’s rich biodiversity, with its varied microclimates and abundant medicinal plants, forms a strong foundation for traditional herbal medicine. Indigenous groups such as the BriBri and Cabécar contributed significantly to this legacy, which has evolved through interactions with European and Afro-Caribbean populations.

Research emphasizes the cultural importance of herbal medicine within the “Pura Vida” lifestyle, …

Perceptions And Attitudes Of Western Michigan University Students On Antibiotic Medications, Emily Boask

Honors Theses

On a global scale, antibiotic resistance is a threat to public health. The causes of antibiotic resistance are multifaceted, including healthcare providers overprescribing antibiotics, antibiotic overuse in farming and food production, and antibiotic misuse or malpractices. The public recognizes antibiotic resistance as a significant problem but there are serious knowledge gaps regarding antibiotic resistance and antibiotic stewardship.

University students are a particularly important subpopulation of the public to understand appropriate antibiotic use because many of these individuals are navigating their health concerns on their own for the first time. Many individuals believe that antibiotics can improve cold symptoms, upper respiratory …

Evaluating The Duckweeds Lemna Minor And Spirodela Polyrhiza As Alternative Aquatic Hosts For The Spread Of The Bacterial Wilt Pathogen, Ralstonia Solanacearum, Irelynn Smith

Honors Theses

Ralstonia solanacearum is a plant pathogen that causes bacterial wilt disease in major crops such as tomatoes and potatoes. Recently, this bacterium was discovered in floating pennywort (Hydrocotyle ranunculoides) and Pennsylvania smartweed (Polygonum pennysylvanicum), two previously unsuspected aquatic plants. Duckweed is an aquatic plant with a high level of resilience and short doubling time. Common duckweed (Lemna minor) has previously demonstrated its capability as a versatile high-yield infection model for other types of pathogenic bacteria. Given this newfound branch of aquatic hosts and the fact that duckweed is a common pond weed previously identified in several ponds located nearby infected …

Geopolitical Shifts In Scandinavia: Analyzing The Diplomatic Trajectories Of Sweden And Finland, Pedro Ruvalcaba

Honors Theses

The geopolitical landscape of Scandinavia has witnessed significant transformations over the past century, bringing nations like Sweden and Finland to the forefront of international relations discourse. This thesis examines the evolving diplomatic strategies of these countries in response to contemporary geopolitical challenges, including the assertiveness of Russia, the rise of global powers such as China and North Korea, and the implications of Iran’s nuclear ambitions. The invasion of Ukraine by Russia in 2022 serves as a critical juncture, prompting Sweden and Finland to reevaluate their long-standing policies of neutrality and non-alignment, ultimately leading to their recent NATO membership.

Tracing the …

From Cells To Clinics- The Role Of College Biology Education In Shaping Future Physician Assistants, Monica Manley

Honors Theses

This thesis provides a personal reflection on translating concepts and techniques learned in multiple biology courses (i.e. microbiology, neuroscience, comparative physiology, and genetics) in to clinical practice as an intern in the emergency room of a hospital. Each selected concept contains a description followed by an example of how this knowledge relates to a specified duty of a physician assistant with in an emergency medical setting.

Cell Type-Specific Production Of Amyloid Precursor Protein During Infection Is Dependent On The Strain Of Herpes Simplex Virus 1, Mia Elias

Honors Theses

Cleavage of amyloid precursor protein (APP) generates the antimicrobial peptide amyloid beta (Aβ), the accumulation of which is linked to Alzheimer’s Disease (AD) pathogenesis. Whether processing of APP contributes to anti-viral responses is debated. Herpes Simplex Virus Type 1 (HSV-1), a neurotropic double-stranded deoxyribonucleic acid (DNA) virus, establishes lifelong latency and can cause a range of outcomes, from asymptomatic infection to herpetic encephalitis (HSE). Whether disparate outcomes are dependent on HSV-1 strain is unknown. Using three differentially virulent HSV-1 strains, we compared the regulation of APP-associated proteolytic processing in both a corneal infection murine model and an in vitro model. …

Determining The Antimicrobial Activity And Permeabilizing Effects Of Polyunsaturated Fatty Acids And Piscidins On Vibrio Parahaemolyticus And Vibrio Vulnificus, Christina Harris

Honors Theses

Antimicrobial peptides (AMPs) are found in all organisms and have gained attention for their effectiveness against a variety of pathogens. Piscidins are AMPs that were discovered in mast cells of vertebrates, mostly within marine animals, and are an important part of the innate immune system due to their potent antimicrobial properties. Piscidin 1 (P1) and piscidin 3 (P3) are found within sea bass and have different antimicrobial activities. P1 exhibits strong membrane disruptive properties, while P3 goes through the membrane to the targeted DNA. These piscidins can experience metalation with copper (Cu2+) which enhances their antimicrobial activity in vitro (P1-Cu2+ …

The Role Of Oncomodulin In The Central Nervous System Inflammation, Evan Paltjon

Honors Theses

Acoustic overexposure in mice leads to an excess of calcium influx in outer hair cells (OHCs) and causes permanent hearing loss due to death of OHCs. Oncomodulin is a parvalbumin calcium buffering protein located in OHCs of the cochlea. Early pilot studies indicate that genetic deletion of OCM results in immune-related pathology throughout the cochlea. Why and how this occurs is unknown. Studies in wild type (WT) and OCM knockout (KO) mice demonstrate greater numbers of immune cells are associated with noise exposure in an OCM knockout than in WT. Expression of toll-like receptor 4 (TLR4) leads to the recruitment …

Production Of Mrna Vaccine For Influenza, Christina Miller

Honors Theses

The objective of this project is to design a process to produce an mRNA influenza vaccine that serves as an alternative to conventional influenza vaccines, replacing current options with innovative mRNA technologies. Given that older populations are most likely to follow up on immunizations consistently, and the increasing average age worldwide, the market for this project appears to be expanding in the next 80 years. Additionally, experts predict more frequent mutations and outbreaks of viral disease from a variety of factors including globalization and global temperature trends. Rapid response to these viral outbreaks will continue to be as, or even …

Benefits Of A Community Reentry Intervention For Reentry Self-Efficacy In Homeless Shelter Residents With Past Incarceration, Kathryn E. Hurley

Honors Theses

The U.S. has one of the highest levels of incarceration and recidivism in the world (American Civil Liberties Union, 2022; U.S. Department of Justice, n.d). Several factors contribute to recidivism, including the loss of monetary funds and loss of housing upon incarceration. Homelessness especially predicts recidivism. It has been found to increase the risk of recidivism by almost 50% (Jacobs & Gottlieb, 2020). Within the context of a long-term ongoing participatory community action research Project in homeless shelters (Reeb et al., 2024), this study examined the benefit of reentry support sessions within the homeless shelter environment, with an attempt to …

Identifying The Effects Of Environmental Conditions On Listeria Monocytogenes Fitness And Pathogenesis Modified By Transcription Factor Cody, Elizabeth K. Herr

Honors Theses

Listeria monocytogenes is a pathogen with the capability of causing severe illness in individuals who consume contaminated foods. Many foods have been found to harbor the bacterium, but dairy products, produce, and other prepackaged foods are particularly susceptible to contamination. Contaminated foods are exposed to a variety of environmental conditions during packaging, processing, consumption, and digestion, all of which play an essential role in modulating the survival and pathogenesis of L. monocytogenes. Conditions of particular interest include cold storage, presence of food additives, and activity of antimicrobial enzymes such as lysozyme. My honors thesis research has focused on elucidating how …

Impacts Of Climate Change On Vector-Borne Disease Burden In The United States: Exploring The Epidemiological Evidence, Leah V. Loetman

Honors Theses

Climate change models predict shifts in vector-borne diseases (VBDs) around the world, including in the United States. However, to my knowledge there has yet to be a comprehensive descriptive epidemiological study examining prevalence, geographic distribution, and trends for all tick- and mosquito-borne diseases in the United States. I compiled data from the U.S. Centers for Disease Control (CDC), which publishes yearly case numbers for 17 vector-borne diseases. However, these publications depend on a scattered framework of state regulations regarding disease reporting. I compiled data from the CDC to determine the current geographic distribution, prevalence, and trends of tick- and mosquito-borne …

Odiar Es Proteger: La Respuesta Inevitable Al Origen De Una Pandemia, Haowen Huang

Honors Theses

Esta tesis analiza la causa del odio hacia los asiáticos durante la pandemia de COVID-19, enfocándose específicamente en los casos que tuvieron lugar en España. La prevalencia de discriminación dirigida al origen de una pandemia identificado por la narrativa del brote es cultivada por la persistencia de estereotipos. En el caso de COVID-19, la xenofobia hacia los asiáticos fue el resultado de una construcción histórica que ha estigmatizado su identidad persistentemente, la repetición de la cual es definida en esta tesis como racismo pandémico. La imagen de los asiáticos enfermos fue establecida injustificadamente por la medicina occidental en el siglo …

Opioid Policy Solutions: Administrative Law, Legislation, And Constitutional Reform, Trevin Stevens

Honors Theses

I have written three separate policy briefs addressing issues in the pharmaceutical industry that allowed for the opioid epidemic to reach the magnitude that it has in the last three decades. I examined public policy regarding labeling, drug diversion, and misuse, as well as marketing. By studying existing policies, I was able to provide insight into possible reforms, while accounting for potential obstacles to systematic reform, such as constitutional concerns regarding free speech.

Cd46 Is A Protein Receptor For Human Adenovirus Type 64, Alexander Michael Robertson

Honors Theses

Adenoviruses are important gene delivery vectors and causative agents for a variety of human diseases such as the common cold and gastrointestinal infections. Human adenovirus type 64 (Ad64; formerly 19c) and type 37 are associated with epidemic keratoconjunctivitis. Based upon its high homology and similar disease tropism to Ad37, we hypothesized that Ad64 would have the same protein receptor, CD46 (membrane cofactor protein), as Ad37. We show that a recombinant Ad64 containing an enhanced Green Fluorescent Protein transgene (Ad64.eGFP) enters Chinese hamster ovary cells expressing human CD46 (CHO-CD46) on the surface. Entry into human cervical carcinoma (HeLa) cells is increased …

Fighting Aids With No Help: Radical Solidarity And How Community Organizations Overcame A Neglectful Federal Government, Sophia Kennan

Honors Theses

Community organizations have forever shaped the history of the United States. As de Tocqueville noted in a visit to the US in the 19th century, volunteerism and forming associations is a way of life ingrained in American society. The way that these community organizations operate in relation to government action is incredibly important to understanding democratic processes in the US, and one such period that is of note is the AIDS crisis of the 1980s. During this time, the government was infamously silent and neglectful on themes surrounding the epidemic, and this inaction greatly informs how community organizations operated during …

Nurses’ Perceptions Of Ppe Compliance Among South Mississippi Medical- Surgical Nurses, Jenna Roush

Honors Theses

Utilizing Personal Protective Equipment (PPE) appropriately is critical in protecting nurses and patients from contracting infectious diseases. Poor compliance with PPE is associated with increased hospital-acquired infections and directly impacts infection control measures. This thesis delves into the perceptions of PPE compliance and awareness among medical-surgical nurses in South Mississippi. A quantitative study utilizing a 28-item survey was implemented. Likert-style and open-ended survey questions were completed by n=28 participants. Univariate analysis was used to determine compliance among each PPE item, awareness and confidence of PPE items, and whether there was a lack of training or a PPE shortage. Based on …

The Effects Of Artificial Sweeteners On The Human Body And Microbiome, Lauren K. Steely

Honors Theses

While artificial sweeteners are becoming a popular sugar alternative, their negative effects remain understudied and obscure to the general public. Whether people purposely incorporate them into their diet, artificial sweeteners are found in thousands of products and are consumed by all ages. The calories and weight gain associated with refined sugar often cause people to turn to zero-calorie options, thinking they are healthier; however, artificial sweeteners serve just as, if not greater, a threat to one’s health. Artificial sweeteners have been associated with weight gain, numerous health risks, type 2 diabetes, and negative effects on the gut microbiome. The goal …

Evaluating The Impact Of Temperature And Artificial Light At Night On The Growth, Swimming Performance, And Corticosterone Levels Of Southern Leopard Frog (Lithobates Sphenocephalus) Tadpoles, Skylar Alexander

Honors Theses

Anthropogenic activities caused by urbanization have considerably altered our world’s ecosystems which has led to a global amphibian population decline. Encroaching and unyielding urbanization by humans has contributed to a rise in artificial light at night due to light pollution as well as increased temperature due to climate change. Both rising temperatures and artificial light at night (ALAN) have been shown to individually have negative effects on amphibian physiology such as increased stress and decreased behavior. However, due to the drastic nature of human imposition, amphibians typically encounter these anthropogenic stressors in combination. Therefore, it is important to understand how …

The Effects Of Aloe Vera On The Human Gut Microbiome, Samantha Leon

Honors Theses

The human gut microbiome is a diverse community of bacteria influenced by multiple factors including host age, geographical location, and diet. This study observed the effects of aloe vera juice on the human gut microbiome. Fecal samples were taken every two weeks over a period of one year; the subject consumed aloe vera juice in alternating cycles of consumption “on” and abstaining “off” with each cycle lasting for eight weeks. The bacterial community in each sample was characterized using 16S rRNA gene sequencing. Sequences were identified and their relative abundances were used to look for patterns distinguishing “on” and “off” …

Botulinum Toxin For Abductor Type Spasmodic Dysphonia: A Systematic Review, Keyona Sutton

Honors Theses

A systematic review was conducted to show that BTX (Botulinum Toxin) is the most effective treatment for Abductor Spasmodic Dysphonia (ABSD). There were a total of 117 records screened for inclusion in this review. After the initial screening, there were seven successive rounds of screening for eligibility. This process produced a total of three eligible records that met the following conditions: patients presented with spastic abduction, electromyography BTX type A injection into the posterior cricoarytenoid muscle, and measurement of results with acoustic dimensions. After surveying all eligible studies, it was concluded that BTX is an effective treatment for ABSD. Every …

A Comprehensive Analysis Of Financial Accounting Through Case Studies, Will Bounds

Honors Theses

This thesis is a collection of accounting case studies. There are eleven cases that comprise this thesis including: 102 Minutes that Changed America, Design Your Life – Nashville, Excel Crash Course, Pfizer Profitability, Business Interview – Hank Bounds, Case Competition – Overview, Case Competition – Audit, Case Competition – Tax, Case Competition – Advisory, Case Competition – Risk, and Case Competition – Presentation. There are a large variety of topics involved in all these cases. All of them have proven to be great learning experiences for myself. Case VI through Case XI deal with the Case Competition that went on …