Digital Commons Network^™

Literature Replication: Insect Gut Cell Isolation Protocols, Sophie Pickelner

Honors Theses

A large problem in science is the lack of literature replication, especially for subjects in which there is little published research. Literature replication is an integral part of the scientific method and is important for validating methods and results of published studies. A study from Nature found that out of 1,500 scientists interviewed, over 70% have been unable to reproduce literature studies and over half of them have been unable to reproduce even their own studies (Baker, 2016). This problem is even more pronounced in the field of biology, with 80% of scientists reporting that they are unable to replicate …

From Big Farm To Big Pharma: A Differential Equations Model Of Antibiotic-Resistant Salmonella In Industrial Poultry Populations, Rilyn Mckallip

Honors Theses

Antibiotics are used in poultry production as prophylaxis, curative treatment, and growth promotion. The first use is as prophylaxis, or prevention of common bacterial diseases. The crowded conditions in concentrated animal feeding operations necessitate management of infectious disease to ensure overall animal health and the profitability of such operations. In these farms, between 20,000 and 125,000 birds are raised in shed-like enclosures [3], with an average of less than one square foot of space per chicken [34]. Antibiotics are currently used in chicken farms to manage and prevent common bacterial diseases such as respiratory and digestive tract infections, as well …

Reflections: A Meditation On Ballet And Pain, Claire Madeline Silverman

Honors Theses

The defining details of the character of Giselle are that she loves to dance even though she knows it could kill her (for she has a frail heart), and that she is in love with a peasant boy named Loys, though she knows her mother dislikes him. She is defiant, determined to follow her desires.

Giselle wasn’t one of the ballets that stuck out to me when I was younger. I loved the Tchaikovsky ballets — Swan Lake, The Sleeping Beauty, The Nutcracker — and later Romeo and Juliet (Macmillan’s version) became my favorite. Giselle existed at my periphery. It’s …

Effects Of Cannabichromene On Pain And Neuroinflammation In Hiv-1 Tat Transgenic Mice, Sydney Wheeler

Honors Theses

HIV-1 TransActivator of Transcription (Tat) is a vital regulatory protein involved in HIV replication and implicated in development of HIV-associated pain and neurocognitive deficits. Tat is thought to contribute to these pathologies through proinflammatory pathways. Developing mechanisms to curb Tat-mediated inflammation is a key step in improving the quality of life of those living with HIV. Both recreational and medicinal cannabis use is prevalent among many people living with HIV; given this prevalence and the perceived anti-inflammatory benefits of cannabis use, it is important to investigate how chemical constituents of cannabis may impact HIV. In the present study, the effects …

Psychological Influences On Problem-Solving Following Lab-Induced Learned Helplessness, Aqdas Khan

Honors Theses

The purpose of the present study was to examine psychological influences on problem-solving following lab-induced learned helplessness. There are several related psychological constructs in the literature that appear relevant. These have been typically examined in isolation in various sub-fields within psychology. Determining which is most influential, and their unique and overlapping contributions, could provide important conceptual clarity and point to ways to streamline and guide targets of psychological intervention. The constructs of interest were: need for cognition, psychological flexibility, grit, learned helplessness (LH) attributional style, cognitive fusion, and the Big 5 personality trait of neuroticism. A within-subjects correlational research group …

Ivermectin: Covid-19 "Miracle Cure?": A Case Study On Public Health Communication To Combat Disinformation, Ashley Dittmar

Honors Theses

Communicating accurate health information to the public has always been a vital role of public health professionals. This role became especially prominent in light of the COVID-19 pandemic, when public health professionals had the challenge of sharing up-to-date information about a novel coronavirus while other messages about the virus - both true and false - were being spread by other entities. This paper focuses on one case of intentionally incorrect information (disinformation) that was spread during the COVID-19 pandemic, purporting anti-parasitic drug ivermectin as a “miracle cure” for COVID-19. This case study is analyzed through the lens of the Media …

The Public Trust Doctrine: Roman Law Roots And Future In Environmental Litigation, Phoebe Liccardo

Honors Theses

The public trust is an ancient and enduring legal doctrine. With roots in Roman law, the public trust persists through English law, and into the United States. Scholars and jurists in the field of Roman and environmental law do not all agree on the history and continuity of the public trust doctrine (PTD). There are those who argue the Roman law introducing a public trust did not assert the same protections present in the doctrine today. Conversely, there are those who believe the Roman law did enshrine similar protections, and further, that it calls for an expansion of PTD in …

A Review Of Pre-Clinical Studies On Meth-Induced Gut Microbiome Alterations, Payton Brabant

Honors Theses

Substance use disorder (SUD) is a chronic, pervasive disease that affects approximately 40.3 million people aged 12 and over within the United States (SAMHSA, 2021). Recent investigations have emphasized preventive measures targeting the development of SUDs and the occurrence of relapse. However, to date, FDA-approved medications are only available for those with alcohol (AUD) and opioid use disorders (OUD) (Forouzan et al., 2021). Given that about 964,000 people have methamphetamine use disorder (MUD) within the United States, investigation of novel treatment approaches for psychostimulant abuse is warranted (Forouzan, Hoffman, and Kosten, 2020). Several negative effects can occur after using methamphetamine …

Emotional Support Animals On Campus: Relevance, Benefits, And Controversies, Grace Campbell

Honors Theses

This research pertains to Emotional Support Animals (ESAs) on university campuses. It explores the need for ESAs (specifically for college students), differentiates between types of support animals, and discusses benefits and controversies that come along with ESAs on campus. Following this, it sets up a research study that can be used at Western Michigan University relating to student experience with ESA utilization and approval. The study, if performed, would give insight to student experiences, and help the University in supporting the maximum number of students. Emotional support animals have many benefits for college students, including but not limited to, a …

Optimization Of Material Flow Of Woodshop Through Layout Redesign, Madison Wegner

Honors Theses

A local high-design outdoor site furniture company – Landscape Forms – worked with the senior design team to create alternative layouts for the woodshop within the company. This senior project will offer three categories of alternative layouts: short, mid, and long term. By offering multiple time frame layouts, Landscape Forms will be able to implement changes based on time frame and need. A project plan indicates all tasks required to meet these objectives as well as a timeline for accomplishing this plan. Research including worker movement diagrams, stakeholder interviews incorporating woodshop employees and engineers, a man and machine utilization study, …

Facing Famine: Justice And The Case Of Unilateral Intervention, Tanner R. Brooks

Honors Theses

Through the course of this year, 900 thousand people will have to struggle through conditions of famine, and a total of 345.2 million will experience food insecurity of some kind. These concerning figures represent an over twofold increase since 2020.1 This presents a serious problem, as access to food is so plainly vital to every aspect of an individual’s existence. It should therefore be uncontroversial to assert the grave nature of the occurrence of famine and other food emergencies faced by so many today. Food emergencies are not merely a result of insufficient food, but rather the institutional policies enacted …

Early Life Δ9-Tetrahydrocannabinol Exposure Of F0 Zebrafish Causes Hyperactivity In F1 Offspring, Jenna Cripe

Honors Theses

As cannabis increases in its accessibility, potency, and acceptance across the United States, investigation into the multigenerational effects of Δ9-tetrahydrocannabinol (THC) exposure during key stages of development is critical. The aim of this study was to evaluate whether there would be behavioral impacts in the F1 offspring following a dose-response of THC exposure (0.08, 0.4, or 1 µM) during development in the F0 generation. Zebrafish (Danio rerio) were utilized in this study. Behaviors (locomotive activity and anxiety-like behavior) in the F1 generation were evaluated at 120 hours post-fertilization (hpf) with the larval photometer response (LPR) assay, and at 3, 11, …

Inhibiting Effects Of Methyllycaconitine (Mla) On Pnu-282987 Induced Neurogenesis In Blast-Exposed Mouse Retinas, Nastia Zhiyew Chan

Honors Theses

Irreversible vision loss can be caused by retinal damage, disease, or aging. In recent years, the prevalence of ocular damage due to blast trauma has been increasing due to the modernization of warfare. Adult mammalian retinal neurons were previously thought to lack the capacity for regeneration to reverse vision loss, which deemed the effects of many ocular damages permanent. However, recent studies from this lab have shown that PNU-282987, an agonist to the alpha 7 subtype of neural nicotinic acetylcholine receptors, will result in neural regeneration in adult mammalian cells. For this research project, I will be using Methyllycaconitine (MLA), …

Connections Through Contrast: The Built Environment Embracing Fine Art Exhibition, Josilyn Welch

Honors Theses

An emotionally immersive experience within a museum setting can foster feelings of belonging, engagement, exploration, understanding and connection. Providing an environment where empathetic immersion can be achieved through physical, psychological, and social enablers lead to transformative experiences for the visitor. To promote physical, psychological, and social enablers within the museum setting to promote empathetic immersion within its visitors, this museum will consider the following strategies:

Physical Enablers: The museum space will address physical enablers by including environmental features such as diverse opportunities for seating, immersive lighting techniques, and curated finish selections for individual exhibit spaces as well as interactive displays …

Membership Probabilities Of White Dwarfs In Open Star Clusters, Sarah Phillips

Honors Theses

This thesis explores the relationship between astronomical position data and star cluster membership. Our research project focuses on the mathematical probability of cluster membership for white dwarf stars (WDs), specifically membership candidates within the open cluster Messier 35. Through Gaussian analysis of proper motion data, we examine the membership probability of each candidate. Establishing cluster membership has broad applications and can allow further determination of a star’s characteristics including stellar age and evolution. Of particular interest are two WD member candidates for Messier 35: LAWDS 4, a DB WD with a helium-dominated atmosphere as opposed to the more common hydrogen-dominated …

Nous Sommes Tous Des « Djadjas » : La Question De L’Identité Dans La Musique Française Et Francophone Au 21^E Siècle, Molly Earle

Honors Theses

Le titre de mon travail fait référence à une chanson qui représente les thèmes de la race,1 du genre, de la transculturalité et de la collaboration musicale : « Djadja », une chanson écrite par la chanteuse franco-malienne Aya Nakamura qui a eu beaucoup de succès en 2018 et puis en 2020 avec le remix en duo avec Maluma, un chanteur colombien. Comme d’autres chansons de Nakamura, « Djadja » utilise des sons africains et urbains, mais l’ajout d’influences latino-américaines a aidé la chanson à toucher une plus grande partie du monde (Nakamura et Maluma). Par exemple, cette chanson est …

The Power Of Citizen Science: Overcoming The Knowledge Gap Of The Marine Mammal Diversity In The Bay Islands Of Honduras, Gloria Joo Ryung Oh

Honors Theses

Preservation of regional biological diversity requires an understanding of the inhabiting species. However, species surveys are often difficult due to a a lack of resources.  This study utilized citizen science, which involves the use of non-scientists for data collection, to summarize the marine mammal sightings  in the Bay Islands of Honduras (BIH). After data filtration, thirteen species of marine mammals were identified in the 509 observations records, of which 92% originated from citizen science sources.  These included four species that did not have a previous record in the BIH, two of which were first reports for the country of Honduras: …

An Examination Of The Vaginal And Endometrial Microbiota Of Heifers And The Effects Of Selenium Supplementation On Microbiome Composition, Kobe S. Perry

Honors Theses

Selenium, an essential trace element, is involved in many biological processes serving catalytic, structural, and regulatory roles through mechanisms that are not entirely understood. Due to its health effects from either deficiency or toxicity, selenium has been well-studied, with an emphasis towards selenium deficiency which has been shown to cause immune and reproductive issues in animals. To address deficiency, different forms of supplementation have been used, yet few studies have examined selenium’s effects on the reproductive microbiome based on the type of supplementation (i.e., inorganic or organic forms). By examining bovine vaginal and endometrial mucosal samples via 16S gene sequencing, …

Legal And Community Input In The Record Of Decision For The Bluegrass Army Depot, Hannah E. Taylor

Honors Theses

The Bluegrass Army Depot of Richmond, Kentucky is owned and operated by the United States Army to demilitarize the chemical weapons stockpile facility. The citizens of Richmond filed a civil suit against the Army for wanting to incinerate the stored chemical weapons. This research compiles an overview of the history of chemical weapons, current laws and legislation regarding the facility, political implications, public opinions surrounding the installation, facility stockpiles and ammunition, chemical weapons dissemination methods, and specific analysis and research dedicated to the Bluegrass Army Depot’s project stages, destruction technologies, and public involvement. The central purpose is to compile a …

Is Lineage C A Distinct Species?, Shiza Khan

Honors Theses

No abstract provided.

Effects Of Actin-Destabilizing Drugs On Motor Function In Drosophila Melanogaster, Caden Sullivan

Honors Theses

Actin dynamics influence many cellular processes including neural connectivity and function. One way to better understand and study the regulation of these dynamics is to manipulate actin dynamics and observe the effects in motor function as a proxy to changes on the neuromuscular junctions in developing neural tissue. On that line, this investigation hypothesized that changing the actin dynamics during development via drug manipulation results in observable effects in locomotion. One of the common actin regulatory drugs used throughout the literature includes Latrunculin A, which was used in this study. Methods included applying this actin destabilizing drug on developing wildtype …

Computational Investigation Of Porphyrin-Based Host Design For Anions And Ion Pairs, Symphony Hill

Honors Theses

Little research has heretofore been devoted to studying the binding interactions between porphyrin-based molecular hosts and anions or ion pairs. Such interactions could be important in synthesizing molecules capable of detecting, binding, extracting, and transporting ions of biological, environmental, and industrial significance. With this in mind, computational studies of nine different porphyrin hosts were performed to examine the structural and binding properties of each molecule and potential ion guests. Using the Spartan computational chemistry software, each porphyrin was modeled and binding was tested with a number of ions of different shapes and sizes. Data was then collected regarding the distances …

Faces Of Faith: Monastic Identity And Protestant Theology In The Swiss Reformation, Kevin O'Gorman

Honors Theses

Religious orders were ever-present in medieval life. Their influence was not limited just to the pulpit and the physical area around monasteries but extended into the daily life of entire kingdoms. Each religious community was unique in the interpretation and expression of its Rule of life, both between and within orders. During the Reformation, religious communities faced pressure from newly converted Protestant authorities alongside theological conversations within their own walls. New Protestant theologies carried with them anti-monastic ideas that challenged religious communities to fundamentally reexamine their lives. Nowhere were these choices as complicated as in Switzerland, where monks and nuns …

Determining The Role Of Propionate And Sigb On Benzalkonium Chloride Resistance In Listeria Monocytogenes, Emma C. Schaefer

Honors Theses

Listeria monocytogenes (L. monocytogenes) is a deadly food borne pathogen that causes listeriosis infection in humans with a high mortality rate. L. monocytogenes can form biofilms in food processing environments and become resistant to antimicrobial agents such as benzalkonium chloride (BC) and other quaternary ammonium chlorides (QACs). BC is used for cleaning and disinfection of food processing areas and is known to disrupt cell membranes of L. monocytogenes, causing cytosol leakage and the degradation of proteins and nucleic acids. Previous research shows that QAC resistance is associated with the upregulation of certain efflux pump genes (mdrL, brcABC, …

Substituent Effect Analysis On Halogen Bonding Interactions, Summer Hanson

Honors Theses

This study was used to determine the significance of substituents placed on aromatic molecules and how electron-donating groups (EDG) and electron-withdrawing groups (EWG) affect the strength of the halogen bond. Each study involved the same substituents, each differing in electron-withdrawing and electron-donating capability. Five different systems were created on computational software to evaluate the distance between the halogen bond donor and halogen bond acceptor. In two studies, the goal was to find the best electron-donor on the halogen bond acceptor (donor of electron density) while the halogen bond donor remained constant. For one study, the goal was to determine the …

Competition Versus Choice: Evolution Along A Narrow Path In Drosophila Β2 Tubulin, Olivia B. Parson

Honors Theses

The Drosophila melanogaster β2 protein (Dmβ2) has sustained a long evolutionary stasis for the last 60 million years (Nielsen 2006). Even small changes to the protein’s primary amino acid sequence render it non-functional, suggesting its stasis may be due to stringency in the structure/function relationship (Nielsen 2001). This project seeks to understand what has prevented Dmβ2 from evolving, with the two main hypotheses being that Dmβ2 either exists as an ideal protein configuration that competitively bests all alternates or that Dmβ2 is the only possible configuration that will support spermatogenesis in Drosophila melanogaster. In order to test these hypotheses, the …

Measuring Wetland Restoration Success Through Water Quality And Invertebrate Community Indices, Abbey E. Raison

Honors Theses

Wetland restoration projects are essential to preserving these imperiled ecosystems. While restoring lost or degraded wetlands is the first step, determining the success of these restoration efforts is often difficult or only focuses on one aspect of an ecosystem (e.g., water quality testing). I plan to measure the success of wetland restoration through traditional (i.e., water quality testing) and non-traditional methods (i.e., terrestrial and aquatic insect sampling) in native (i.e., control) and restored (i.e., experimental) wetlands in Ohio. I will use sweep nets for aboveground insect collection and a dip net for aquatic macroinvertebrate collection. These samples will be sorted, …

Identifying The Effects Of Anaerobicity And Propionate On Listeria Monocytogenes Metabolism And Central Nervous System Infection, Rebecca Rudd

Honors Theses

Listeria monocytogenes is a facultative foodborne pathogen that can enter the bloodstream and invades the central nervous system to cause meningitis. As an intracellular pathogen, L. monocytogenes replicates inside the host cell cytosol and avoids extracellular immune defenses as it disseminates throughout the body. L. monocytogenes can also reach and cross the blood brain barrier, resulting in severe or fatal symptoms in immunocompromised and elderly patients. The overarching goal of my research project is to better understand how different environmental factors, anaerobicity and propionate, in the intestinal lumen alter the ability of L. monocytogenes to cause infections. In the first …

Making Good Doctors: The Ama’S Code Of Ethics And A Culture Of Virtue, John Howard Hassmann

Honors Theses

The American Medical Association’s (AMA) Code of Medical Ethics consists of principles that ensure the legal sanctity and professional conduct of practicing physicians. These principles outline imperatives for physicians “primarily for the benefit of the patient. But a physician can be terrible without violating the AMA’s Code of Medical Ethics. Although the AMA’s code ought not be forsaken or replaced, any code of ethics cannot make its adherents good. Physicians cannot become good following a code that neglects to address the delicacy of good habits. Further, a topical, crisis-management approach to ethical training stifles physicians whose ethical goals transcend lawfulness …

Use Of Edna To Determine The Presence Of Cryptosporidium Spp. In Freshwater Sources In Harlan, Kentucky, Madison Hall

Honors Theses

Cryptosporidium spp. oocysts are transmitted via a fecal-oral route, e.g., water sources, and the parasite is a leading cause of waterborne disease in the United States. Research into Cryptosporidiosis in rural areas is lacking. This study utilized eDNA methods to determine if Cryptosporidium spp. was present in Harlan, Kentucky, an underserved area in Appalachia. We used a pump-powered commercially available Smith-Root Citizen Scientist eDNA (Vancouver, WA) system to filter freshwater sources and collect eDNA. Samples were collected from tap and spring water as controls and three times from five locations on the three tributaries of the Cumberland River: Martins Fork …