Digital Commons Network^™

Analyzing Multigene Stacking And Genome Editing Strategies In Rice, Bhuvan Pathak

Graduate Theses and Dissertations

Crop improvement through biotechnology is an integrated effort, incorporating multiple approaches like integration of genes, editing of native genes, and removal of selection marker genes. Before streamlining the protocols, the efficiency and feasibility of the individual approach and their components must be tested. This study evaluated following approaches: 1) stacking an array of genes into a single locus by site-specific integration via Cre-lox recombination in rice, 2) determining the efficiency of I-SceI and the CCR5-ZFN in the targeted excisions of gene fragments in rice and Arabidopsis, and 3) determining the efficiency of CRISPR/Cas9 in generating targeted mutations for genome editing …

Schooling And Civic Behavior: A Global Perspective, Ian Kingsbury

Graduate Theses and Dissertations

National governments confront different challenges to the goal of creating model citizens, as well as different ambitions in the type of citizen that they wish to create. The United States government faces a tension in determining the role of education in shaping the social order. As a liberal democracy that extols the virtue of individual liberty, the United States should allow educational pluralism to flourish. Paradoxically, however, a nation of immigrants might require an education system that turns students into “proper Americans” who honor the precepts of liberty, equality, and self-government. I draw from domestic and international studies to inform …

Optimizing Block-Stacking Operations With Relocation, Hueon Lee

Graduate Theses and Dissertations

The focus of the dissertation is developing the optimization problem of finding the minimum-cost operational plan of block stacking with relocation as well as devising a solution procedure to solve practical-sized instances of the problem. Assuming changeable row depth instead of permanent row depth, this research is distinguished from conventional block stacking studies.

The first contribution of the dissertation is the development of the optimization problem under the assumption of deterministic demand. The problem is modeled using integer programming as a variation of the unsplittable multi-commodity flow problem. To find a good feasible solution of practical-sized instances in reasonable time, …

Contributions Of Role-Playing Games: Advantages Of Incorporating Social Media In Disaster Response, Erin Mullin

Graduate Theses and Dissertations

After a disaster, emergency managers need to know who needs help, what type of help they need, and how soon they need it. Traditionally, they have relied on 911 calls and ground assessments to collect this information. Because the needs of a population are not able to be identified in a timely manner by ground assessments and because individuals are often unable to get through to 911, many civilians in distress turn to social media outlets as a last-ditch effort to obtain the services they need. Due to the uncertainty concerning the accuracy of social media posts, responding to disaster …

Cost Effectiveness Of Greenhouse Gas Reductions Through Afforestation Of Agricultural Land In The Arkansas Delta, Karli A. Moore

Graduate Theses and Dissertations

Sequestration of atmospheric carbon in forested lands offsets carbon emissions from other industries. Conversion of private lands, particularly agricultural tracts in marginal areas, to forests can bolster carbon abatement. The United States government agencies administer some voluntary, incentive-based programs to encourage landowners to adopt production practices with positive environmental outcomes. This policy stream can be used to increase transition of marginal agricultural land to forests, thereby creating new carbon sinks. We analyze an eleven-county study area in the Arkansas Delta to determine feasibility for a subsidy focused on carbon abatement through afforestation. This study area is significant for two reasons: …

Production And Purification Of Basic Fibroblast Growth Factor Fused To Two Collagen Binding Domains Expressed In E. Coli Bl21 Using Flask And Fed-Batch, Hazim Aljewari

Graduate Theses and Dissertations

Delivering effective and non-toxic doses of bioactive materials that can aid in activating tissue regeneration to wounded tissue has proven to be an enormous challenge. This study was designed to produce a potential therapeutic recombinant protein by fusing two collagen binding domains to basic fibroblast growth factors (bFGF) through a collagenase cleavage site linker, so it can release the bFGF in a wound site by the action of this enzyme. The novel fusion protein was expressed in Escherichia coli BL-21 (E. coli) using traditional flask shaker and fed-batch cultivation. Cell lysate was purified by FPLC using Immobilized metal affinity chromatography …

Extracting Social Network From Literary Prose, Tarana Tasmin Bipasha

Graduate Theses and Dissertations

This thesis develops an approach to extract social networks from literary prose, namely, Jane Austen’s published novels from eighteenth- and nineteenth- century. Dialogue interaction plays a key role while we derive the networks, thus our technique relies upon our ability to determine when two characters are in conversation. Our process involves encoding plain literary text into the Text Encoding Initiative’s (TEI) XML format, character name identification, conversation and co-occurrence detection, and social network construction. Previous work in social network construction for literature have focused on drama, specifically manually TEI-encoded Shakespearean plays in which character interactions are much easier to track …

Characterizing Hydrothermal System Groundwater Dynamics Using Conservative And Non-Conservative Tracers: Insights And Implications Drawn From Study At The Taupo Volcanic Zone (New Zealand) And Valles Caldera (Usa), Joshua Michael Blackstock

Graduate Theses and Dissertations

Hydrothermal systems constitute an important component of heat and mass transport within Earth’s crust having direct and indirect impacts to society. Investigating contemporary hydrochemical differences and change are critical to evaluating and deconvolving factors that influence geothermal systems through time. In this dissertation, groundwater mixing approaches were used to study hydrochemical differences in two globally significant hydrothermal areas, which included fabrication of a low-cost dissolved CO2 monitoring for dissolved inorganic carbon (DIC). In the Taupo Volcanic Zone (New Zealand), empirical spatial relations were used to derive low-temperature groundwater end-members of Cl and Cl/Br for end-member mixing analysis (EMMA). Using EMMA, …

Interfacial Contact With Noble Metal - Noble Metal And Noble Metal - 2d Semiconductor Nanostructures Enhance Optical Activity, Ricardo Raphael Lopez Romo

Graduate Theses and Dissertations

Noble metal nanoparticles and two-dimensional (2D) transition metal dichalcogenide (TMD) crystals offer unique optical and electronic properties that include strong exciton binding, spin-orbital coupling, and localized surface plasmon resonance. Controlling these properties at high spatiotemporal resolution can support emerging optoelectronic coupling and enhanced optical features. Excitation dynamics of these optical properties on physicochemically bonded mono- and few-layer TMD crystals with metal nanocrystals and two overlapping spherical metal nanocrystals were examined by concurrently (i) DDA simulations and (ii) far-field optical transmission UV-vis spectroscopic measurements. Initially, a novel and scalable method to unsettle van der Waals bonds in bulk TMDs to prepare …

Understanding And Controlling Lithium Microstructure During Electroplating For Energy Applications, Yang Tian

Graduate Theses and Dissertations

Lithium-ion batteries are reaching the specific theoretical capacity limit, while lithium metal batteries are regarded as the ideal energy storage system for the next generation “beyond lithium-ion” battery systems. The lithium metal anode is considered as the “Holy grail” of anodes due to its relatively low electrochemical potential (-3.04 V vs SHE) and high theoretical capacity (3860 mAh g-1). However, the application of lithium metal anodes is hindered because of significant reaction between metallic lithium and electrolytes, as well as uneven electro-plating, which leads to dendrite formation, causing safety problems.

The kinetic parameters of Li ions such as diffusion coefficient …

Surface Modification Of Pvdf Membranes For Treating Produced Waters By Direct Contact Membrane Distillation, Zahra Anari

Graduate Theses and Dissertations

Direct contact membrane distillation is a promising unit operation for treating hydraulic fracturing flow back and produced water. However, while a hydrophobic membrane is essential to prevent the passage of water from the feed to the permeate side, fouling by dissolved organic species can compromise membrane performance and result in wetting of the membrane pores. Here four monomers, hydroxyethylmethacrylate, acrylic acid, 1-vinyl-3-allylimidazolium bromide, and 1-vinyl-3-hexylimidazolium bromide have been grafted from the surface of a PVDF membrane. The modified and base membranes were tested in a direct contact membrane distillation system. All membranes were challenged with real produced water. In addition, …

Fractional Order State Feedback Control For Improved Lateral Stability Of Semi-Autonomous Commercial Heavy Vehicles, Rasheed Mazen Abdulkader

Graduate Theses and Dissertations

With the growing development of autonomous and semi-autonomous large commercial heavy vehicles, the lateral stability control of articulated vehicles have caught the attention of researchers recently. Active vehicle front steering (AFS) can enhance the handling performance and stability of articulated vehicles for an emergency highway maneuver scenario. However, with large vehicles such tractor-trailers, the system becomes more complex to control and there is an increased occurrence of instabilities. This research investigates a new control scheme based on fractional calculus as a technique that ensures lateral stability of articulated large heavy vehicles during evasive highway maneuvering scenarios. The control method is …

Theoretical Investigations Of The Electronic, Magnetic, And Thermoelectric Properties Of Transition-Metal Based Compounds, Haleoot Edaan Raad

Graduate Theses and Dissertations

The electronic, magnetic, and thermoelectric properties of transition-metal based compounds were investigated by using the density functional theory and Boltzmann transport formalism. It was found that the Co-based Heusler compounds and InSe monochalcogenide are among the materials that may be used for future thermoelectric devices. Furthermore, the investigation showed that the quaternary Heusler compounds, such as, CoFeYGe, where Y is Ti or Cr, are half-metallic ferromagnetic materials with full electron spin polarization. The lattice thermal conductivity (κL) was found to decrease for these alloys as the temperature increases. The present investigation indicated that the phonon optical modes have a major …

Exploring Convergence Of Snake Skin-Inspired Texture Designs And Additive Manufacturing For Mechanical Traction, Catherine Sue Tiner

Graduate Theses and Dissertations

This research focuses on the understanding, development, and additive manufacture of a 3D printed snake skin-inspired texture pattern. The design functionalities of snake skin were determined through the study of the snake species Python Regius otherwise known as the ball python. Each scale of a snake has hierarchical texture with hexagonal macro-patterns aligned on the ventral surface of the skin with overriding anisotropic micro textured patterns such as denticulations and fibrils. Using a laser-powder bed fusion (L-PBF) process, 420 stainless steel samples were 3D printed which closely resemble the above described directional texture of natural snake skin. This printed surface …

Influence Of Single And Multiple Histidine Residues And Their Ionization Properties On Transmembrane Helix Dynamics, Orientations And Fraying, Fahmida Afrose

Graduate Theses and Dissertations

Since aromatic and charged residues are often present in various locations of transmembrane helices of integral membrane proteins, their impacts on the molecular properties of transmembrane proteins and their interactions with lipids are of particular interest in many studies. In this work, I used solid-state deuterium NMR spectroscopy in designed model peptide GWALP23 [GGALW(LA)6LWLAGA] with selective deuterium labels to addresses the pH dependence and influence of single and multiple “guest” histidine residues in the orientation and dynamic behaviors of transmembrane proteins. The mutations include Gly to His (G2/22 to H2/22), Trp to His (W5/19 to H5/19) and Leu to His …

Recovered Images: Medieval Echoes In C. S. Lewis’S Space Trilogy, Nathan Earl Houston Fayard

Graduate Theses and Dissertations

C. S. Lewis has begun to garner more scholarly attention in the last few decades, but his first novels, his science fiction or Space trilogy, continue to be largely ignored by academia. Yet, these three novels are deserving of more serious study, as they are pioneering works of literary science fiction, and even more surprisingly, of literary medievalism. Though long derided as mere reactionary attacks on Modernism and science, when properly understood, these strange and wonderful tales actually reveal the complexity and nuance of Lewis’s response to his times. In them, the Inkling author creates a unique combination of the …

Sentiment Analysis, Quantification, And Shift Detection, Kevin Labille

Graduate Theses and Dissertations

This dissertation focuses on event detection within streams of Tweets based on sentiment quantification. Sentiment quantification extends sentiment analysis, the analysis of the sentiment of individual documents, to analyze the sentiment of an aggregated collection of documents. Although the former has been widely researched, the latter has drawn less attention but offers greater potential to enhance current business intelligence systems. Indeed, knowing the proportion of positive and negative Tweets is much more valuable than knowing which individual Tweets are positive or negative. We also extend our sentiment quantification research to analyze the evolution of sentiment over time to automatically detect …

Evaluation And Analysis Of Null Convention Logic Circuits, John Davis Brady

Graduate Theses and Dissertations

Integrated circuit (IC) designers face many challenges in utilizing state-of-the-art technology nodes, such as the increased effects of process variation on timing analysis and heterogeneous multi-die architectures that span across multiple technologies while simultaneously increasing performance and decreasing power consumption. These challenges provide opportunity for utilization of asynchronous design paradigms due to their inherent flexibility and robustness.

While NULL Convention Logic (NCL) has been implemented in a variety of applications, current literature does not fully encompass the intricacies of NCL power performance across a variety of applications, technology nodes, circuit scale, and voltage scaling, thereby preventing further adoption and utilization …

Nematode Populations As Affected By Residue And Water Management In A Long-Term Wheat-Soybean Double Crop In Eastern Arkansas, Lucia Emperatriz Escalante Ortiz

Graduate Theses and Dissertations

Soybeans (Glycine max) are one of the major row crops in the United States, particularly in Arkansas. Soybean cyst nematode (SCN, Heterodera glycines) and southern root-knot nematode (RKN, Meloidogyne incognita) are two of the most damaging pests that cause major economic losses in soybeans. Little is known concerning the effects of common and alternative agronomic practices on nematodes in fields with nematode population densities below threshold levels. Therefore, the objective of this study was to evaluate the effects of the combination of tillage (conventional tillage and no-tillage), irrigation (irrigated and non-irrigated), wheat (Triticum aestivum) residue burning (burned and no burned), …

Synthesis Of Cellulose Nanocrystal-Gold Nanoparticle Hybrid System For Surface Plasmon-Enhanced Property, Mahshid Iraniparast

Graduate Theses and Dissertations

Gold nanoparticles (AuNPs) have been brought to the forefront of various applications, ranging from theranostics, to organic photovoltaics, to biosensing owing to their localized surface plasmon resonance (LSPR) property. However, this property needs to be improved in order to allow for high sensitivity and quantitative detection of biomolecules. Hybrids of AuNPs with low-dielectric cellulose nanocrystal (CNCs) would yield enhancement of the LSPR property, which is driven by the confinement of electron oscillation at their interfaces. This study proposed a seed-mediated growth method to synthesize hybrids of CNCs-AuNPs. Sulfate groups on the surface of CNCs served as the sites for the …

Women In Stem: Strategies And Recommendations For Academic Women And Institutional Leaders, Sarah Jensen

Graduate Theses and Dissertations

Women still comprise a small number of full professors in STEM disciplines in research universities, which have historically been male dominated. The National Science Foundation (NSF) has recognized the challenge of getting more women to enter the professoriate, earn tenure, and advance to full professor. These women can then encourage more women to enter into STEM as educators and researchers. The purpose of this study was to recognize, explore, and depict strategies used by women full professors to overcome obstacles they faced while advancing in academic STEM fields. The study participants also offered recommendations for women faculty desiring to become …

Mitigating Herbicide Impacts To Soybean, Grant Lawson Priess

Graduate Theses and Dissertations

The rapidity in evolution of herbicide-resistant weeds and the resulting cost to U.S. farmers demonstrate the need to responsibly steward the limited number of herbicides available in agricultural systems. To reduce weed emergence and likewise added selection pressures placed on herbicides, early-season crop canopy formation has been promoted. However, impacts to soybean following a potentially injurious herbicide application have not been thoroughly evaluated. Therefore, field experiments were conducted to determine whether: 1) soybean injury from metribuzin or flumioxazin delayed canopy formation or changed the incidence of pathogen colonization; 2) residual herbicides applied preplant reduced the potential for soybean injury and …

Quantitative Analysis Techniques For Assessing Organelle Organization And Dynamics In Individual Cells, Isaac Vargas

Graduate Theses and Dissertations

In biomedical optics and microscopy, the organization and morphology of organelles have been widely studied. In spite of novel imaging techniques, there is still a lack of quantitative tools to easily measure cellular characteristics from image data. Previous studies have explored multiple approaches to assess organelle organization and alignment, resulting in complicated and extensive algorithms that are both subject to multiple steps of image processing and influenced by non-cellular artifacts. In this thesis, a technique called the Modified Blanket Method (MBM) is introduced to quantify organelle organization through measurements of fractal dimension (FD) on a pixel-by-pixel basis. With the use …

A Geospatial Analysis Of Safety And Risk Perception In The Buffalo National River, Julie Nicole Terhune

Graduate Theses and Dissertations

In 1972, the Buffalo River in Arkansas became America’s First National River. Since then, the Buffalo National River has become a beloved recreational retreat for locals and visitors alike. The Buffalo National River is home to incredibly scenic views and host to many outdoor activities, among them, hiking and floating. As with any confluence of nature and humans, there is great risk involved in all aspects of the park. Between 2011 and 2017, a total of 140 search and rescue missions were carried out. Some were resolved easily, but more serious accidents were not unheard of.

Risk perception studies are …

Studies Of Initial Growth Of Gan On Inn, Alaa Alnami

Graduate Theses and Dissertations

III-nitride materials have recently attracted much attention for applications in both the microelectronics and optoelectronics. For optoelectronic devices, III-nitride materials with tunable energy band gaps can be used as the active region of devices to enhance the absorption or emission. A such material is indium nitride (InN), which along with gallium nitride (GaN) and aluminum nitride (AlN) embody the very real promise of forming the basis of a broad spectrum, a high efficiency solar cell. One of the remaining complications in incorporating InN into a solar cell design is the effects of the high temperature growth of the GaN crystal …

Applications Of Ionic Liquids In Membrane Separation, Mohanad Kamaz

Graduate Theses and Dissertations

Ionic liquids represent an emerging attractive material in membrane technology. The central theme of this doctoral dissertation is to develope novel membranes using ionic liquids. Two different approaches were used to prepare ionic liquid membranes including the immobilization of ionic liquid within the membrane pores or the use of pressure assembly method to deposit a selective ionic liquid layer on the top membrane surface.

In chapter 2, imidazolium ionic liquids with three different alkyl halides were successfully synthesized and used to prepare supported ionic liquid membranes (SILMs). SILMs preraper were tested for aqueous and nonaqueous applications. For nonaqueous applications, the …

Improving Earthquake Disaster Models With Post-Event Data: Insights From The 2015 Gorkha, Nepal Earthquake, Bradley Steven Wilson

Graduate Theses and Dissertations

Immense amounts of data are collected following earthquake disasters. Yet, it remains unclear how researchers’ might take full advantage of diverse post-disaster datasets. Using data from the 2015 Gorkha Nepal earthquake, this dissertation explores three ways in which post- disaster survey and assessment datasets can be used to inform models of seismic risk, vulnerability, and recovery processes. The first article presents an empirical analysis of scale issues in disaster vulnerability indices using a novel dataset of 750,000 households. This study finds that using aggregated household data to create social vulnerability indices can produce results that are meaningfully different from equivalent …

Quantitative Study Of The Antimicrobial Effects Of Silver On The Motility Of Escherichia Coli, Benjamin Russell

Graduate Theses and Dissertations

In recent decades, the number of antibiotic-resistant bacterial infections has grown to become a serious global threat. This rise can be attributed to the widespread misuse of antibiotics and the lack of newly developed drugs to fight resistant organisms. Novel bactericidal substances have, therefore, garnered significant research interest. Silver, due to its powerful antimicrobial effects, is one such substance. Silver is typically most effective in cationic form; however, advancements in nanotechnology have paved the way for the controlled fabrication of nano-silver. Silver nanoparticles have been shown to have increased antibacterial potency for a variety of reasons, including the release of …

Assessing The Repeatability Of Clinical Tests In People With And Without Flexion-Induced Neck Pain, Ashton Human

Graduate Theses and Dissertations

The accessibility of mobile technology has improved productivity but can contribute to musculoskeletal disorders due to the posture associated with using such devices. Neck flexion, a posture assumed by smartphone users, has been shown as a risk factor for the development of clinical neck pain if sustained for prolonged periods. Determining the individuals who are naturally more predisposed to develop flexion-induced neck pain can aid in the prevention and treatment of musculoskeletal disorders. The purpose of this thesis is to assess the repeatability of clinical tests between trials over the course of two days in young adults who do and …

The Van Hiele Analysis Of Curricular Materials: A Comparative Study, Vinay Kumar Kalyankar

Graduate Theses and Dissertations

The goal of this study is to investigate K-8 geometry curriculum standards and textbooks for their effectiveness in preparing students for high school geometry. The basis for the study is van Hiele theory pioneered by the Dutch educators and researchers of geometric understanding in adolescents, Dina van Hiele Geldof and Pierre van Hiele. Another driving force for this dissertation is research into mathematics textbooks and curriculum standards of school mathematics as seen in CCSSM and state specific standards documents, both of which influence the mathematics students have the opportunity to learn.

The K-8 geometry curriculum standards and textbook instructional segments …